{"product_id":"15-sds-page-gel-preparation-kit","title":"15% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation requires multiple manual procedures, including precise acrylamide concentration adjustment, separate preparation of resolving and stacking gels, polymerization reagent handling, and repeated optimization for different protein sample types. These steps can increase preparation time and introduce variation, especially when high-resolution separation of small proteins or peptides is required.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0261)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for high-resolution separation of low molecular weight proteins and peptide samples using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complex reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, the RP0261 \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e contains a 15% polyacrylamide gel formulation with smaller pore sizes for improved resolution of small proteins compared with lower percentage SDS-PAGE gels. It is particularly suitable for applications requiring enhanced separation of proteins below approximately 50 kDa, including detailed protein characterization and high-resolution Western blot analysis.\u003c\/p\u003e\n\u003cp\u003eThe colored concentrating gel included in this \u003cstrong\u003eprotein gel casting kit\u003c\/strong\u003e improves visualization of sample loading wells and supports accurate sample application during electrophoresis. Compared with conventional hand-cast SDS-PAGE methods, the \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation workflow\u003c\/strong\u003e reduces preparation complexity and improves consistency for laboratories performing routine and advanced protein analysis.\u003c\/p\u003e\n\u003cp\u003eThe RP0261 kit is intended for research use only and is suitable for applications including \u003cstrong\u003esmall protein separation, peptide electrophoresis, Western blot gel preparation, protein expression analysis, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0261\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, small protein separation, Western blotting\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized 15% SDS-PAGE gel formulation:\u003c\/strong\u003e Provides a high-percentage polyacrylamide matrix with smaller pores for improved separation of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-resolution protein separation:\u003c\/strong\u003e The 15% SDS-PAGE gel improves band resolution for small proteins and peptide samples compared with lower percentage gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-formulated solutions simplify gel casting by reducing manual calculations and preparation procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free gel preparation:\u003c\/strong\u003e The optimized coagulant system simplifies polymerization without requiring additional TEMED addition during gel preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid PAGE gel preparation:\u003c\/strong\u003e Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein resolution:\u003c\/strong\u003e The pre-optimized formulation helps reduce variability associated with conventional hand-cast SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/strong\u003e is designed for laboratories requiring high-resolution separation of small proteins and peptide samples using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSmall protein separation:\u003c\/strong\u003e Suitable for resolving low molecular weight proteins that require higher percentage SDS-PAGE gels for improved separation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePeptide electrophoresis:\u003c\/strong\u003e Provides enhanced resolution for peptide samples and very small protein fragments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for detection of low molecular weight proteins before membrane transfer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein characterization:\u003c\/strong\u003e Analysis of purified proteins, protein fragments, and molecular weight differences.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein expression analysis:\u003c\/strong\u003e Evaluation of recombinant proteins and engineered protein products with lower molecular weights.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiotechnology and academic research:\u003c\/strong\u003e Convenient high-resolution SDS-PAGE gel preparation for molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eThe optimal SDS-PAGE gel concentration depends on protein molecular weight and the required separation resolution. Lower percentage polyacrylamide gels provide larger pores for high molecular weight proteins, while higher percentage gels provide improved resolution for smaller proteins. The \u003cstrong\u003e15% SDS-PAGE gel\u003c\/strong\u003e contains a higher acrylamide concentration and smaller pore structure, making it suitable for high-resolution separation of small proteins, protein fragments, and peptide samples.\u003c\/p\u003e\n\u003ctable cellpadding=\"8\" cellspacing=\"0\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/6-one-step-page-color-gel-quick-preparation-kit?variant=52775633911989\"\u003e6% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/8-sds-page-gel-preparation-kit?variant=52775642595509\"\u003e8% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/10-sds-page-gel-preparation-kit?variant=52775673626805\"\u003e10% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/12-sds-page-gel-preparation-kit?variant=52775704723637\"\u003e12% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e15% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPREPARATION WORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0261 \u003cstrong\u003eOne-Step SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e provides a simplified workflow for preparing high-resolution protein electrophoresis gels. The pre-optimized formulation reduces manual preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel mixture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the separation gel solution and gently mix to initiate polymerization while avoiding bubble formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the prepared resolving gel solution into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix Concentrating Gel Solution and Concentrating Gel Buffer to prepare the stacking gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant and directly cast the concentrating gel above the separation gel layer without waiting for complete resolving gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow approximately 15 minutes for gel polymerization before performing SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe simplified casting procedure reduces preparation complexity and improves reproducibility for laboratories performing high-resolution separation of small proteins and peptide samples.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0261 kit is designed for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e. Separation and concentrating gel buffers contain SDS and are not intended for native PAGE applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe 15% polyacrylamide formulation provides smaller pore sizes than lower percentage SDS-PAGE gels, making it suitable for enhanced resolution of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe colored concentrating gel improves visualization of sample loading wells and supports accurate sample application during electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-percentage SDS-PAGE gels may provide improved separation of small proteins but may require optimization of electrophoresis conditions depending on sample properties.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGel polymerization speed is temperature dependent. Lower temperatures may extend polymerization time, while suitable room temperature conditions support efficient gel formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor optimal electrophoresis performance, freshly prepared gels are recommended for use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e \u003cstrong\u003eManual\u003c\/strong\u003e \u003c\/a\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/15_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783474528\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003ca href=\"#\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a 15% SDS-PAGE Gel Preparation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eA 15% SDS-PAGE Gel Preparation Kit is designed for high-resolution separation of small proteins, protein fragments, and peptide samples. The RP0261 kit provides a high-percentage polyacrylamide gel formulation suitable for applications requiring improved resolution of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy choose a 15% SDS-PAGE gel instead of a 12% gel?\u003c\/summary\u003e\n\u003cp\u003eA 15% SDS-PAGE gel contains a higher acrylamide concentration and smaller pores compared with a 12% gel. This provides improved separation capability for smaller proteins and peptide samples that may not be sufficiently resolved using lower percentage gels.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0261 be used for Western blot analysis?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0261 can be used for Western blot workflows requiring separation of low molecular weight proteins before membrane transfer and antibody-based detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for a 15% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0261 15% SDS-PAGE gel is recommended for small proteins, protein fragments, and peptide samples generally below approximately 50 kDa. Actual separation performance depends on electrophoresis conditions and sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RP0261 require additional TEMED during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eNo. RP0261 uses an optimized coagulant system that simplifies SDS-PAGE gel preparation without requiring additional TEMED addition during the casting process.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a high-percentage SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eHigh-percentage SDS-PAGE gels provide smaller pore sizes, allowing improved separation resolution for lower molecular weight proteins and peptides compared with lower percentage polyacrylamide gels.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0261 compatible with standard SDS-PAGE electrophoresis equipment?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0261 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between RP0261 15% gel and RP0251 12% gel?\u003c\/summary\u003e\n\u003cp\u003eRP0251 12% SDS-PAGE gel provides routine protein separation with improved resolution for many commonly analyzed proteins, while RP0261 15% SDS-PAGE gel is optimized for smaller proteins and peptide samples requiring higher resolution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775732707509,"sku":"RP0261","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/15_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783475951","url":"https:\/\/biofargo.com\/products\/15-sds-page-gel-preparation-kit","provider":"Biofargo","version":"1.0","type":"link"}