{"product_id":"15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation","title":"15 mL \/ 50 mL Lymphocyte Separation Tube(Empty), with Porous Barrier, for PBMC Isolation","description":"\u003cstyle\u003e\n    .bf-container {\n        font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\n        color: #333;\n        line-height: 1.6;\n        max-width: 1200px;\n        margin: 0 auto;\n        padding: 20px;\n    }\n\n    \/* Section Headers *\/\n    .bf-container h2 {\n        \n        padding-left: 15px;\n        background: #f8f9fa;\n        padding-top: 10px;\n        padding-bottom: 10px;\n        margin-top: 30px;\n        font-size: 1.5rem;\n        color: #0056b3;\n        text-transform: uppercase;\n        letter-spacing: 0.5px;\n    }\n \/* Highlight Box for Notes *\/\n    .bf-note-box {\n        background-color: #fff4e5;\n        border: 1px solid #ffcc80;\n        padding: 15px;\n        border-radius: 4px;\n        margin-bottom: 20px;\n    }\n  \n    \/* Table Styling *\/\n    .bf-table-wrapper {\n        overflow-x: auto;\n        margin: 20px 0;\n    }\n\n    .bf-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 1rem;\n        background-color: transparent;\n    }\n\n    .bf-table th, .bf-table td {\n        padding: 12px 15px;\n        border: 1px solid #dee2e6;\n        text-align: left;\n    }\n\n    .bf-table tr:nth-child(even) {\n        background-color: #fcfcfc;\n    }\n\n    \/* Images and Captions *\/\n    .bf-image-container {\n        text-align: center;\n        margin: 30px 0;\n    }\n\n    .bf-image-container img {\n        max-width: 100%;\n        height: auto;\n        border: 1px solid #eee;\n        border-radius: 8px;\n        box-shadow: 0 2px 10px rgba(0,0,0,0.05);\n    }\n\n    .bf-caption {\n        display: block;\n        font-style: italic;\n        color: #666;\n        margin-top: 10px;\n        font-size: 0.9rem;\n    }\n\n    \/* Lists *\/\n    .bf-container ul {\n        padding-left: 20px;\n    }\n\n    .bf-container li {\n        margin-bottom: 10px;\n    }\n\n    @media (max-width: 768px) {\n        .bf-table td:first-child {\n            width: 35%;\n        }\n    }\n\u003c\/style\u003e\n\n\u003cdiv class=\"bf-container\"\u003e\n\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cp\u003eThe cell separation tube is designed for the isolation of lymphocytes and mononuclear cells from whole blood. A porous sieve plate is integrated inside the tube to prevent premature mixing of the blood sample with the separation medium below the barrier, thereby reducing contamination of upper-layer cells by erythrocytes and facilitating easier recovery of the separated cells.\u003c\/p\u003e\n    \u003cp\u003eThis product utilizes the density gradient centrifugation method. Based on differences in cell density, a separation medium and centrifugation are applied to achieve cell separation and purification. The density gradient separation medium forms a defined gradient after loading anticoagulated whole blood into the tube. Following centrifugation, erythrocytes and granulocytes sediment to the bottom of the tube, while PBMCs (peripheral blood mononuclear cells, including lymphocytes and monocytes) remain at the interface above the separation medium. A small fraction of cells may remain suspended within the medium. Lymphocytes can be collected by aspirating the cell layer at the interface.\u003c\/p\u003e\n    \u003cp\u003eThis product offers advantages including ease of operation, absence of cytotoxicity, high cell recovery, and excellent cell viability.\u003c\/p\u003e\n\n    \u003ch2\u003eSpecifications\u003c\/h2\u003e\n    \u003cdiv class=\"bf-table-wrapper\"\u003e\n        \u003ctable class=\"bf-table\"\u003e\n            \u003cthead\u003e\n                \u003ctr style=\"background-color: #0056b3; color: #fff;\"\u003e\n                    \u003cth style=\"text-align:center;\"\u003eProduct Name\u003c\/th\u003e\n                    \u003cth style=\"text-align:center;\"\u003eSKU\u003c\/th\u003e\n                    \u003cth style=\"text-align:center;\"\u003eTube Volume\u003c\/th\u003e\n                    \u003cth style=\"text-align:center;\"\u003ePacking\u003c\/th\u003e\n                \u003c\/tr\u003e\n            \u003c\/thead\u003e\n            \u003ctbody\u003e\n                \u003ctr\u003e\n                    \u003ctd style=\"text-align:center;\"\u003eCell Separation Tube\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e7072031\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e15 mL\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e20 tubes\/box, 10 boxes\/carton\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd style=\"text-align:center;\"\u003eCell Separation Tube\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e7072041\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e50 mL\u003c\/td\u003e\n                    \u003ctd style=\"text-align:center;\"\u003e15 tubes\/box, 10 boxes\/carton\u003c\/td\u003e\n                \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n        \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cp\u003eStore unopened at room temperature. Shelf life is 3 years.\u003c\/p\u003e\n\n    \u003ch2\u003eInstructions for Use\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eLoading of Separation Medium\u003c\/strong\u003e\u003cbr\u003eUnder aseptic conditions in a clean bench, open the cell separation tube and place it on a tube rack. Add separation medium into each tube:\u003c\/p\u003e\n            \u003cul\u003e\n                \u003cli\u003e\u003cp\u003e15 mL tube: 4–4.5 mL\u003c\/p\u003e\u003c\/li\u003e\n                \u003cli\u003e\n\u003cp\u003e50 mL tube: 15–15.5 mL\u003c\/p\u003e\n\u003cp\u003eTighten the cap securely.\u003c\/p\u003e\n\u003c\/li\u003e\n            \u003c\/ul\u003e\n        \u003c\/li\u003e\n\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eCentrifugation\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eCentrifuge at 1000 × g for 1 minute at 20°C.\u003c\/p\u003e\n        \u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cdiv class=\"bf-image-container\"\u003e\n        \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231115101855_480x480_121530f2-1e50-4b06-827e-8d530aa24c65.jpg?v=1776844321\" target=\"_blank\"\u003e\n            \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231115101855_480x480_121530f2-1e50-4b06-827e-8d530aa24c65.jpg?v=1776844321\" alt=\"\" style=\"cursor: zoom-in;\"\u003e\n        \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003cul\u003e\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eInspection\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eCheck whether the separation medium has completely passed through the sieve plate and filled the lower chamber. If not, add additional medium and repeat centrifugation.\u003c\/p\u003e\n        \u003c\/li\u003e\n\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eSample Loading\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eAdd anticoagulated whole blood directly without dilution:\u003c\/p\u003e\n            \u003cul\u003e\n                \u003cli\u003e\u003cp\u003e15 mL tube: 3–6 mL\u003c\/p\u003e\u003c\/li\u003e\n                \u003cli\u003e\u003cp\u003e50 mL tube: 15–25 mL\u003c\/p\u003e\u003c\/li\u003e\n            \u003c\/ul\u003e\n        \u003c\/li\u003e\n\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eCentrifugation\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eCentrifuge at 800 × g for 15 minutes at 20°C.\u003c\/p\u003e\n            \u003cp\u003eUse low acceleration and deceleration settings (e.g., gear 3 if 10 levels are available).\u003c\/p\u003e\n        \u003c\/li\u003e\n\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003ePBMC Collection\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eAfter centrifugation, the PBMC layer is located between the plasma and the separation medium. Carefully aspirate the PBMC layer.\u003c\/p\u003e\n        \u003c\/li\u003e\n\n        \u003cli\u003e\n            \u003cp\u003e\u003cstrong\u003eWashing Step\u003c\/strong\u003e\u003c\/p\u003e\n            \u003cp\u003eWash the collected cells 1–2 times using RPMI-1640 medium (20°C, 250 × g, 10 min). Resuspend lymphocytes in 0.9% (w\/v) saline or appropriate culture medium for downstream use.\u003c\/p\u003e\n        \u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eApplicable Instruments\u003c\/h2\u003e\n    \u003cp\u003eHorizontal rotor centrifuge.\u003c\/p\u003e\n\n    \u003ch2\u003eSample Requirements\u003c\/h2\u003e\n    \u003cp\u003eThe sample must be fresh anticoagulated blood collected under sterile conditions. Freezing or refrigeration during storage, handling, or transportation must be avoided.\u003c\/p\u003e\n\n    \u003ch2\u003eInterpretation of Results\u003c\/h2\u003e\n    \u003cp\u003eVariations in centrifuge performance and environmental temperature conditions may affect separation efficiency. Users may optimize centrifugation speed and time to achieve optimal results according to their laboratory conditions.\u003c\/p\u003e\n\n    \u003ch2\u003eLimitations of the Method\u003c\/h2\u003e\n    \u003cp\u003eThe procedure requires that the temperature of the sample, separation medium, and environment be maintained at (20 ± 5)°C under normal atmospheric conditions\u003c\/p\u003e\n\n    \u003ch2\u003ePrecautions\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003e\u003cp\u003eIf the separation tube is opened but not immediately filled, ensure the cap is tightly closed to prevent contamination.\u003c\/p\u003e\u003c\/li\u003e\n        \u003cli\u003e\u003cp\u003eAfter filling the separation medium and centrifugation, if not used immediately, keep the tube upright, protected from light at room temperature, and use within 3 months.\u003c\/p\u003e\u003c\/li\u003e\n        \u003cli\u003e\u003cp\u003eThe optimal separation temperature is (20 ± 5)°C. Deviation may affect performance.\u003c\/p\u003e\u003c\/li\u003e\n        \u003cli\u003e\u003cp\u003eBlood should be collected and processed at room temperature, stored in an anticoagulant container for no more than 2 hours, and should not be refrigerated at 4°C.\u003c\/p\u003e\u003c\/li\u003e\n        \u003cli\u003e\n            \u003cp\u003eWhole blood can be used directly without dilution; dilution does not significantly affect separation performance.\u003c\/p\u003e\n            \u003cp\u003eRecommended sample volumes:\u003c\/p\u003e\n            \u003cul\u003e\n                \u003cli\u003e\u003cp\u003e15 mL tube: ≥3 mL, recommended ≤6 mL\u003c\/p\u003e\u003c\/li\u003e\n                \u003cli\u003e\n\u003cp\u003e50 mL tube: ≥15 mL, recommended ≤25 mL\u003c\/p\u003e\n\u003cp\u003eInsufficient sample volume may compromise separation efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n            \u003c\/ul\u003e\n        \u003c\/li\u003e\n        \u003cli\u003e\u003cp\u003eBlood cells separated from plasma may be used directly or diluted (1:1) with calcium- and magnesium-free PBS, saline, or culture medium before loading into the tube, provided sufficient erythrocytes are present.\u003c\/p\u003e\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eRelated Product Options:\u003c\/strong\u003e\n\u003cp\u003eSelect the appropriate format for your PBMC isolation workflow. Choose from separation medium, empty tubes, or ready-to-use prefilled tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px; margin-top: 20px;\"\u003e\n\u003c!-- Empty Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eEMPTY TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003ePBMC Separation Tube (Empty)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation?variant=52452569186485\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Human Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eHuman Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/human-lymphocyte-separation-medium?variant=43275893735605\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Mouse Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eMouse Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/mouse-lymphocyte-separation-medium?variant=43275988598965\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Prefilled Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003ePREFILLED TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eLymphocyte Separation Tube (Prefilled)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/lymphocyte-separation-tube-for-human-peripheral-blood?variant=43275829674165\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"15mL \/ 10Boxes\/Carton(200Tubes\/Carton)","offer_id":52452569186485,"sku":"7072031","price":185.0,"currency_code":"USD","in_stock":true},{"title":"50mL \/ 10Boxes\/Carton(150Tubes\/Carton)","offer_id":52452569219253,"sku":"7072041","price":233.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation","provider":"Biofargo","version":"1.0","type":"link"}