Description

Traditional SDS-PAGE gel preparation involves multiple manual procedures, including accurate acrylamide concentration adjustment, separate preparation of stacking and resolving gels, polymerization reagent handling, and repeated optimization for different protein samples. These steps may increase preparation time and introduce variation in gel performance, particularly when researchers require consistent resolution of lower molecular weight proteins and routine protein analysis results.

The 12% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0251) is a complete SDS-PAGE gel preparation kit designed for efficient preparation of medium-to-high percentage polyacrylamide gels used in protein electrophoresis applications. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complicated reagent calculations.

Designed for the Tris-glycine SDS-PAGE system, the RP0251 One-Step PAGE Gel Kit provides a 12% polyacrylamide gel formulation optimized for routine protein separation requiring improved resolution of lower molecular weight proteins compared with lower percentage gels. The balanced pore structure makes it suitable for common Western blot workflows, protein expression analysis, and molecular weight characterization.

The colored concentrating gel included in this protein gel casting kit improves visualization of sample loading wells and supports accurate sample application during electrophoresis. Compared with conventional hand-cast SDS-PAGE methods, the TEMED-free SDS-PAGE gel preparation workflow reduces preparation complexity and improves consistency between experiments.

The RP0251 kit is intended for research use only and is suitable for applications including Western blot gel preparation, protein expression analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis.

Specification

Product Name	12% One-Step SDS-PAGE Color Gel Fast Preparation Kit
SKU	RP0251
Gel Type	Polyacrylamide SDS-PAGE Gel
Gel Concentration	12%
Electrophoresis System	Tris-Glycine SDS-PAGE System
Recommended Protein Range	Approximately 10–100 kDa proteins
Application	Protein electrophoresis, Western blotting, protein separation
Gel Preparation Method	One-step gel casting workflow
Polymerization Time	Approximately 15 minutes
Storage Condition	4 °C; Novel Coagulant long-term storage at −20 °C
Shelf Life	18 months
Research Use	For Research Use Only (RUO)

Kit Components

Component	Volume
Concentrating Gel Solution	80 mL
Concentrating Gel Buffer	80 mL
Separation Gel Solution	250 mL
Separation Gel Buffer	250 mL
Novel Coagulant	8 mL

Features

Optimized 12% SDS-PAGE gel formulation: Provides enhanced separation resolution for routine protein analysis and proteins requiring smaller pore sizes.
Improved resolution for lower molecular weight proteins: The 12% polyacrylamide gel structure supports clearer separation of proteins commonly analyzed in Western blot experiments.
One-step SDS-PAGE gel preparation: Pre-formulated gel solutions simplify PAGE gel casting by reducing manual calculations and preparation steps.
TEMED-free gel preparation: The optimized coagulant system simplifies polymerization procedures without additional TEMED addition.
Rapid PAGE gel preparation: Gel polymerization can be completed in approximately 15 minutes, improving laboratory workflow efficiency.
Consistent protein separation: The pre-optimized formulation helps reduce variability caused by conventional manual SDS-PAGE gel preparation.

Applications

The 12% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for laboratories requiring reliable protein separation with improved resolution for routine polyacrylamide gel electrophoresis applications.

Western blot analysis: Preparation of SDS-PAGE gels for protein transfer and antibody-based detection workflows.
Routine protein separation: Suitable for resolving commonly studied proteins requiring higher resolution than lower percentage SDS-PAGE gels.
Protein expression analysis: Evaluation of recombinant protein expression and cellular protein profiles.
Protein characterization: Analysis of purified proteins, protein mixtures, and molecular weight distribution.
Cell lysate analysis: Separation of proteins from biological samples before downstream detection.
Biotechnology and academic research: Convenient SDS-PAGE gel preparation for routine molecular biology workflows.

Choosing the Right SDS-PAGE Gel Percentage

The selection of SDS-PAGE gel concentration directly affects protein migration and separation resolution. Lower percentage polyacrylamide gels provide larger pores for high molecular weight proteins, while higher percentage gels provide improved resolution for smaller proteins. The 12% SDS-PAGE gel offers a smaller pore structure compared with 8% and 10% gels, making it suitable for routine protein separation where enhanced resolution of lower molecular weight proteins is required.

Gel Concentration	Recommended Application	Typical Protein Range
6% SDS-PAGE Gel	High molecular weight proteins and large protein complexes	>100 kDa proteins
8% SDS-PAGE Gel	Medium to high molecular weight protein separation	Approximately 50–200 kDa proteins
10% SDS-PAGE Gel	General protein electrophoresis and routine Western blot workflows	Approximately 20–150 kDa proteins
12% SDS-PAGE Gel	Routine protein separation with improved resolution for smaller proteins	Approximately 10–100 kDa proteins
15% SDS-PAGE Gel	Small protein, peptide, and low molecular weight protein analysis	<50 kDa proteins

Preparation Workflow

The RP0251 One-Step SDS-PAGE Gel Preparation Kit provides a simplified gel casting workflow compared with traditional SDS-PAGE preparation procedures. The pre-optimized formulation reduces manual preparation steps while maintaining compatibility with standard Tris-glycine protein electrophoresis systems.

Mix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.
Add Novel Coagulant to the separation gel mixture and gently mix to initiate gel polymerization.
Transfer the prepared separation gel solution into the gel cassette.
Prepare the stacking gel solution by mixing Concentrating Gel Solution and Concentrating Gel Buffer.
Add Novel Coagulant and directly cast the concentrating gel above the separation gel layer without waiting for complete resolving gel polymerization.
Insert the comb and allow approximately 15 minutes for gel polymerization before SDS-PAGE electrophoresis.

This rapid PAGE gel preparation workflow helps laboratories improve efficiency and reproducibility for routine protein separation experiments.

Technical Notes

The RP0251 kit is designed for denaturing SDS-PAGE electrophoresis. Both separation and concentrating gel buffers contain SDS and are not intended for native PAGE workflows.
The 12% polyacrylamide formulation provides increased resolving capability for lower molecular weight proteins compared with lower percentage SDS-PAGE gels.
The colored concentrating gel improves visualization of sample loading wells and supports more accurate sample application during electrophoresis.
Polymerization speed may vary depending on environmental temperature. Lower temperatures may require additional polymerization time.
For optimal protein separation performance, freshly prepared gels are recommended before electrophoresis.

Documents

Manual

MSDS

FAQ

What is a 12% SDS-PAGE Gel Preparation Kit used for?

A 12% SDS-PAGE Gel Preparation Kit is designed for preparing polyacrylamide gels with improved resolution for routine protein separation. RP0251 is commonly used for Western blotting, protein expression analysis, and molecular weight characterization of proteins in the approximate 10–100 kDa range.

Why choose a 12% SDS-PAGE gel instead of a 10% gel?

A 12% SDS-PAGE gel contains a higher acrylamide concentration and smaller pore size compared with a 10% gel. This provides improved resolution for lower molecular weight proteins and samples requiring clearer separation of closely sized protein bands.

Can RP0251 be used for Western blot experiments?

Yes. RP0251 is suitable for Western blot workflows requiring SDS-PAGE protein separation before membrane transfer and antibody-based detection. The 12% gel concentration is commonly selected for routine protein analysis with enhanced band resolution.

Does RP0251 require additional TEMED during gel preparation?

No. RP0251 contains optimized gel preparation reagents and a Novel Coagulant system that simplifies polymerization without requiring additional TEMED addition during PAGE gel casting.

What proteins are suitable for a 12% SDS-PAGE gel?

The RP0251 12% SDS-PAGE gel is suitable for many routine protein analysis applications involving proteins approximately 10–100 kDa. Actual separation performance depends on electrophoresis conditions and sample characteristics.

What are the advantages of a One-Step PAGE Gel Kit?

A One-Step PAGE Gel Kit simplifies SDS-PAGE gel preparation by providing pre-optimized solutions that reduce manual calculations, reagent preparation steps, and variability between experiments.

Is RP0251 compatible with standard SDS-PAGE electrophoresis systems?

Yes. RP0251 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.

What is the difference between RP0251 12% gel and RP0241 10% gel?

RP0241 10% SDS-PAGE gel provides a broad and balanced separation range for general protein analysis, while RP0251 12% SDS-PAGE gel offers improved resolution for smaller proteins and routine protein separation requiring higher gel concentration.

12% One-Step PAGE Color Gel Quick Preparation Kit