The 1-Step 2X RT-PCR Master Mix-HP (High Processibility) is used for qualitative and quantitative RT-PCR amplifications with SYBR Green dye.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.
For the reverse transcription step, this kit uses a highly efficient thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase with optimal temperatures of 60-62°C, and it can be heat-inactivated at ≥90°C.
The thermophilic enzyme highly efficiently synthesizes a complementary DNA strand on RNA template, one unit per 20 μL of reaction.
The kit can detect single digit copies of target RNA.

1-Step 2X RT-PCR Master Mix-SYBR Green Dye-HY, Cat RT-PCR 141