Intended use

The 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates.
The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.

Product Characteristics

The kit is designed for multiplex RT-PCR with TaqMan probes.
For the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.
The RTase is easily heat-inactivated at ≥90°C for 1min.
The RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, ≥1 unit per 20μL of reaction.
The RTase reversely-transcribes single digit copies of target RNA molecules consistently.
The kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.
Up to four pairs of gene-specific primers can be applied in one reaction.
The concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).
The preferable PCR product size is ≤150bp.
The kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.