Description

Glycerophosphoric acid disodium salt hydrate, also known as BGP, is a versatile compound with applications across various research domains, including cell biology, biochemical research, and metabolomics. Recognized as an endogenous metabolite and a serine-threonine phosphatase inhibitor, BGP is extensively utilized in chitosan hydrogel formulations for drug delivery research. It serves as a phosphate source for cell growth and recombinant protein production, offering advantages in media formulations by reducing precipitation at higher pH levels. Additionally, BGP finds applications in micro-arc oxidation electrolyte solutions, contributing to the creation of photocatalytic coatings.

In cell biology, β-Glycerophosphate disodium salt plays a pivotal role as a phosphate group donor in matrix mineralization studies and accelerates calcification in vascular smooth muscle cells. It also promotes bone matrix mineralization when delivered to osteoblasts, providing a crucial source of phosphate ions. In the realm of biochemical research, BGP functions as a classical serine-threonine phosphatase inhibitor in kinase reaction buffers, offering broad-spectrum inhibition. Its role as an organic phosphate donor is particularly valuable in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. Moreover, BGP is utilized to buffer M17 media for Lactococcus culture in recombinant protein expression. The comprehensive utility of BGP spans multiple areas of research, making it an essential component in studies encompassing cell biology, biochemical research, and metabolomics.

Specifications

Synonym	BGP; Glycerol 2-phosphate disodium salt hydrate
CAS Number	154804-51-0
Empirical Formula (Hill Notation)	C₃H₇Na₂O₆P · xH₂O
Molecular Weight	216.04 (anhydrous basis)
EC Number	212-464-3
MDL Number	MFCD00149082
Linear Formula	(HOCH₂)₂CHOP(O)(ONa)₂ · xH₂O
Product Type	Chemical
Purity	≥99% (titration)
mp	102-104 °C (lit.)
UNSPSC	12352201

Features

Versatile and adaptable for a wide variety of laboratory and research applications
Suitable for Cell culture and Plant cell culture
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
BioUltra Grade for your Cell Biology and Biochemical research

Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Properties

Anion Traces	chloride (Cl-): ≤0.05%
Anion Traces	sulfate (SO₄^2-): ≤0.05%
Assay	≥99% (titration)
Biological Source	synthetic (chemical)
Cation Traces	Al: ≤0.001%
	Ca: ≤0.001%
	Cu: ≤0.0005%
	Fe: ≤0.001%
	K: ≤0.005%
	Mg: ≤0.0005%
	Pb: ≤0.001%
	Zn: ≤0.0005%
Color	white
Form	powder
Impurities	≤0.1% Insoluble matter
Impurities	≤0.5 mol % L-α-isomer
InChI	1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
InChI Key	ROPZSVKNEIIIDE-UHFFFAOYSA-L
mp	102-104 °C (lit.)
Product Line	BioUltra
Quality Level	400
SMILES String	O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
Solubility	water: soluble
Technique(s)	cell culture \| mammalian: suitable
Technique(s)	cell culture \| plant: suitable

Safety Info.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

β-Glycerophosphate Disodium Salt Hydrate