{"title":"Western Blot Buffers","description":"\u003cp\u003eWestern blot buffers are critical reagents used to optimize protein transfer, blocking, and detection in Western blotting applications. Designed for reliable and reproducible results, these buffers support efficient protein analysis, ensuring accurate identification and quantification in research and diagnostics.\u003c\/p\u003e","products":[{"product_id":"sds-page-protein-loading-buffer-5-dakewe","title":"SDS-PAGE Protein Loading Buffer 5x - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eModel \u0026amp; Size\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-17-34_600x600.png?v=1717654671\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSDS-PAGE Protein Loading Buffer 5× kit is a 5-fold concentrated protein loading buffer. The main \u003c\/span\u003e\u003cspan\u003ecomponents of this buffer are SDS, DTT, bromophenol blue and salt solution buffer. This product is \u003c\/span\u003e\u003cspan\u003ediluted to 1× when used, and the sample will sink easily and the color will be clear.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Dissolve SDS-PAGE Protein Loading Buffer 5× at room temperature or water bath \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(37℃~40℃). \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Mix protein sample, buffer, and sterilized ddH\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003eO to a final concentration of 1×. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Heat at 100°C or boiling water bath 5-10 minutes to fully denature the protein.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Store at room temperature immediately after dissolving in the water bath to avoid prolonged \u003c\/span\u003e\u003cspan\u003ewater baths. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. This product contains a small amount of DTT, please take precautions when handling it. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Avoid repeated freezing and thawing, and repack the solution into small portions for storage. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. This product is only used for scientific research by professionals, for your safety and health, \u003c\/span\u003e\u003cspan\u003eplease wear a lab coat and disposable gloves.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-20-08_600x600.png?v=1717654825\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at -25°C~-15°C, valid for 12 months. Avoid repeated freezing and thawing. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on blue ice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015011_801501X_SDS-PAGE_Protein_Loading_Buffer_5x-_-C0-A4.pdf?v=1717654892\" type=\"application\/pdf\" target=\"_blank\"\u003eSDS-PAGE Protein Loading Buffer 5×\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015011_801501X_SDS-PAGE_Protein_Loading_Buffer_5x_MSDS.pdf?v=1717654892\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSDS-PAGE Protein Loading Buffer 5×-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"1 mL","offer_id":44250323288245,"sku":"8015011","price":5.56,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015011_SuperDrop_SDS-PAGE_Protein_Loading_Buffer_5x_2cb956ce-9272-4ef1-8606-4ff8c574f988.png?v=1732882257"},{"product_id":"tris-glycine-sds-running-buffer-10-dakewe","title":"Tris-Glycine SDS Running Buffer 10× - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eTris-Glycine SDS Running Buffer 10× is an electrophoresis buffer reagent used in denaturing \u003c\/span\u003e\u003cspan\u003eprotein polyacrylamide gel electrophoresis experiments (SDS-PAGE). This product is prepared \u003c\/span\u003e\u003cspan\u003eusing conventional methods and is 10 × concentrated solution, easy to store and operate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eComponent concentration\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e0.25M Tris, 1.92 M Glycine, 1%\u003c\/span\u003e\u003cspan\u003e（\u003c\/span\u003e\u003cspan\u003eW\/V\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003cspan\u003eSDS, pH8.5.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product is a 10× concentrated solution. Dilute it to 1× working concentration before use: take \u003c\/span\u003e\u003cspan\u003e100 mL of Tris-Glycine SDS Running Buffer (10×), add distilled or deionized water to adjust the \u003c\/span\u003e\u003cspan\u003evolume to 1000 mL, and mix well.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. The prepared electrophoresis solution should not be used for more than two weeks. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. The recovered electrophoresis solution can be reused 1-2 times, but in order to obtain the best \u003c\/span\u003e\u003cspan\u003eelectrophoresis effect, the electrophoresis solution should be prepared for immediate use. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. This product is only used for scientific research by professionals, for your safety and health, \u003c\/span\u003e\u003cspan\u003eplease wear a lab coat and disposable gloves.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at 2°C~8°C, valid for 12 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015021_8015023_801502X_Tris-Glycine_SDS_Running_Buffer_10x-_-C0-A4.pdf?v=1717655591\" type=\"application\/pdf\" target=\"_blank\"\u003eTris-Glycine SDS Running Buffer 10×\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015021_8015023_Tris-Glycine_SDS_Running_Buffer_10_X_MSDS_Ver_1.0.pdf?v=1717655591\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eTris-Glycine SDS Running Buffer 10×-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"500ml","offer_id":44250336166069,"sku":"8015021","price":44.0,"currency_code":"USD","in_stock":true},{"title":"5 L","offer_id":44250342129845,"sku":"8015023","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015021_10x_60a8da41-db3f-4f16-86fd-98c52183bb57.png?v=1732885372"},{"product_id":"fast-western-blot-transfer-buffer-10-dakewe","title":"Fast Western Blot Transfer Buffer 10x - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eFast Western Blot Transfer Buffer 10× is a transfer buffer used in Western blot. This buffer \u003c\/span\u003e\u003cspan\u003egenerates low heat, has a fast transfer rate, and has a good transfer effect on macromolecular \u003c\/span\u003e\u003cspan\u003eproteins that are difficult to transfer. At the same time, this buffer can shorten the time difference \u003c\/span\u003e\u003cspan\u003ebetween small molecule proteins and large molecule proteins for membrane transfer, and achieve \u003c\/span\u003e\u003cspan\u003eefficient transfer of proteins of different molecular weights on the same membrane.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. According to the buffer volume required in the transfer tank, prepare 1× Transfer Buffer in the \u003c\/span\u003e\u003cspan\u003eratio of Transfer Buffer 10× : methanol\/ethanol: water = 1:2:7, mix well and set aside. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Prepare the equipment, transfer membrane and gel required for transfer. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Add 1× Fast Western Blot Transfer Buffer to the transfer tank, set the electrophoresis apparatus \u003c\/span\u003e\u003cspan\u003eto a constant current of 400 mA (voltage is approximately in the range of 75 V~85 V), and start \u003c\/span\u003e\u003cspan\u003etransfer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. This product is suitable for transfer of NC membrane and PVDF membrane. The transfer time \u003c\/span\u003e\u003cspan\u003erequired for NC membrane is about 10-20 minutes, and the transfer time required for PVDF \u003c\/span\u003e\u003cspan\u003emembrane is about 20-30 minutes. The above data is for reference only. Please adjust the transfer \u003c\/span\u003e\u003cspan\u003etime appropriately according to the actual situation during the experiment to achieve the best \u003c\/span\u003e\u003cspan\u003eresults. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. It is suggested that the transferred PVDF membrane be soaked in methanol for a few seconds \u003c\/span\u003e\u003cspan\u003ebefore being dyed with Ponceau red to achieve better results. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. The prepared transfer solution can be reused 2-3 times. It is recommended to increase the \u003c\/span\u003e\u003cspan\u003etransfer time by 2-3 minutes for each reuse. The time required for different electrophoresis \u003c\/span\u003e\u003cspan\u003einstruments is different, please refer to the electrophoresis instrument used in the experiment. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. To prevent the temperature from being too high, please transfer the membrane in an ice bath. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. This product is only used for scientific research by professionals, for your safety and health, \u003c\/span\u003e\u003cspan\u003eplease wear a lab coat and disposable gloves.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\"\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at 2℃~30℃ for 24 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNormal temperate transportation.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015071_8015072_Fast_Western_Blot_Transfer_Buffer_10x-_-C0-A4.pdf?v=1717656792\" type=\"application\/pdf\" target=\"_blank\"\u003eFast Western Blot Transfer Buffer 10×\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015071_8015072_Fast_Western_Blot_Transfer_Buffer_10x_MSDS_Ver_1.0.pdf?v=1717656792\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eFast Western Blot Transfer Buffer 10×-MSDS\u003c\/span\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003etransfer buffer\u003c\/div\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500ml","offer_id":44250359955637,"sku":"8015071","price":52.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015071_10x_9e09ca97-fcaf-4fb8-9245-5473dcfb0726.png?v=1732872072"},{"product_id":"superdissol-instant-mops-buffer-dakewe","title":"SuperDissol Instant MOPS Buffer - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eInstant MOPS Buffer is white to off-white instant granules, and each pack of this product can \u003c\/span\u003e\u003cspan\u003eproduce 1 L of 1 ×MOPS Buffer, which is easy to operate and use. The main components of MOPS \u003c\/span\u003e\u003cspan\u003eare Tris, MOPS, EDTA and SDS. The concentration of Tris, MOPS and EDTA in 1 × MOPS Buffer \u003c\/span\u003e\u003cspan\u003eis 50 mM, 50 mM and 1 mM respectively, and the content of SDS is 0.1%. \u003c\/span\u003e\u003cspan\u003eInstant MOPS Buffer is a commonly used protein gel eletrophoresis buffer in biological \u003c\/span\u003e\u003cspan\u003eexperiments. It is suitable for general SDS-PAGE protein electrophoresis, especially PAGE gel for \u003c\/span\u003e\u003cspan\u003ethe Bis-Tris system. It is matched with Bis-Tris protein gel and suitable for electrophoresis of \u003c\/span\u003e\u003cspan\u003emedium to large molecular weight proteins.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The pH value of 1× solution is 7.0±0.2@25°C.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instruction\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant MOPS Buffer. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar \u003c\/span\u003e\u003cspan\u003euntil it is completely dissolved. (This step can also be performed with magnetic stirrer) \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Transfer the MOPS solution in Step 2 into a constant volume container, and add water to dilute to \u003c\/span\u003e\u003cspan\u003e1 L, which is 1 × working solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_15-11-11_1024x1024.png?v=1717657884\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\" alt=\"\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature of 10°C~30°C, protected from light, with a shelf life of 36 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015091_8015092_SuperDissol_Instant_MOPS_Buffer_-C2-A4.pdf?v=1717658078\" type=\"application\/pdf\" target=\"_blank\"\u003eSuperDissol Instant MOPS Buffer\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015091_SuperDissol_Instant_MOPS_buffer_MSDS.pdf?v=1717658078\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSuperDissol Instant MOPS Buffer-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c!----\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display: none;\"\u003emops-01\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"10 packs\/kit","offer_id":44250429522101,"sku":"8015091","price":134.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015091_SuperDissol_Instant_MOPS_Buffer_1.png?v=1732884067"},{"product_id":"superdissol-instant-tgs-buffer-dakewe","title":"SuperDissol Instant TGS Buffer - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSuperDissol Instant TGS Buffer is white to off-white instant granules, and each pack of this product \u003c\/span\u003e\u003cspan\u003ecan produce 1 L of 1 ×TGS Buffer, which is easy to operate and use. The main components of TGS \u003c\/span\u003e\u003cspan\u003eare Tris, glycine and SDS. The concentration of Tris and glycine in 1 ×TGS Buffer is 25 mM and \u003c\/span\u003e\u003cspan\u003e192 mM, and the content of SDS is 0.1%. Instant TGS Buffer is commonly used as electrophoresis \u003c\/span\u003e\u003cspan\u003ebuffer for SDS-PAGE.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The pH value of 1× solution is 8.3±0.2@25℃.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instruction\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant TGS Buffer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar \u003c\/span\u003e\u003cspan\u003euntil it is completely dissolved. (This step can also be performed with magnetic stirrer) \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Transfer the TGS solution in Step 2 into a constant volume container, and add water to dilute to 1 \u003c\/span\u003e\u003cspan\u003eL, which is 1 × working solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_15-22-30_1024x1024.png?v=1717658571\" alt=\"\" style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_15-22-30_1024x1024.png?v=1717658571\" data-mce-style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\" alt=\"\" style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\" data-mce-style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature of 10°C~30°C, protected from light, with a shelf life of 36 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015101_8015102_SuperDissol_Instant_TGS_Buffer_-C2-A4.pdf?v=1717658722\" type=\"application\/pdf\" target=\"_blank\"\u003eSuperDissol Instant TGS Buffer\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015101_SuperDissol_Instant_TGS_buffer_MSDS.pdf?v=1717658723\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSuperDissol Instant TGS Buffer-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"10 packs\/kit","offer_id":44250512228533,"sku":"8015101","price":82.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015101_SuperDissol_Instant_TGS_Buffer_1.png?v=1732884072"},{"product_id":"superdissol-instant-hepes-buffer-dakewe","title":"SuperDissol Instant HEPES Buffer - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eInstant HEPES Buffer is white to off-white instant granules, and each pack of this product can \u003c\/span\u003e\u003cspan\u003eproduce 1 L of 1×HEPES Buffer, which is easy to operate and use. The main components of \u003c\/span\u003e\u003cspan\u003eHEPES are Tris, HEPES, EDTA and SDS. The concentration of Tris, MES and EDTA in 1×HEPES \u003c\/span\u003e\u003cspan\u003eBuffer is 50 mM, 50 mM and 2 mM respectively, and the content of SDS is 0.1%. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eInstant HEPES Buffer is a kind of protein gel electrophoresis buffer commonly used in biological \u003c\/span\u003e\u003cspan\u003eexperiments, and is often used in biochemical diagnostic kits, DNA\/RNA extraction kits and PCR \u003c\/span\u003e\u003cspan\u003ediagnostic kits. No toxic effects on cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The pH value of 1 × solution is 7.7±0.2@25°C.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instruction\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant HEPES \u003c\/span\u003e\u003cspan\u003eBuffer. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar \u003c\/span\u003e\u003cspan\u003euntil it is completely dissolved (This step can also be performed with magnetic stirrer). \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Transfer the HEPES solution in Step 2 into a constant volume container, and add water to dilute \u003c\/span\u003e\u003cspan\u003eto 1 L, which is 1 × working solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_15-31-18_1024x1024.png?v=1717659094\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature of 10°C~30°C, protected from light, with a shelf life of 36 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015171_8015172_SuperDissol_Instant_HEPES_buffer_-C2-A4.pdf?v=1717659213\" type=\"application\/pdf\" target=\"_blank\"\u003eSuperDissol Instant HEPES Buffer\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015171_SuperDissolInstant_HEPES_buffer_MSDS.pdf?v=1717659213\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSuperDissol Instant HEPES Buffer-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"10 packs\/kit","offer_id":44250542604469,"sku":"8015171","price":134.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015171_SuperDissol_Instant_HEPES_Buffer_1.png?v=1732884061"},{"product_id":"spectblue-protein-staining-reagent-dakewe","title":"SpectBlue Protein Staining Reagent - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSpectBlue Protein Staining Reagent is a fast, highly sensitive, low-background, low-toxic \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCoomassie Brilliant Blue replacement solution. This product does not contain methanol, acetic acid \u003c\/span\u003e\u003cspan\u003eand trichloroacetic acid. The formula is environmentally friendly and has no irritating smell. There \u003c\/span\u003e\u003cspan\u003eis no need to use harmful solvents during decolorization. Can be used for rapid staining of proteins \u003c\/span\u003e\u003cspan\u003ein SDS-PAGE gels without the need for toxic destaining solutions. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis product has high sensitivity and it only takes at least 15 minutes to observe a 10 ng protein \u003c\/span\u003e\u003cspan\u003eband. Appropriate extension of the staining time can further improve the sensitivity. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eClear protein bands can be observed immediately after staining, and destaining with pure water can \u003c\/span\u003e\u003cspan\u003efurther reduce the background and improve the signal-to-noise ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv\u003e\u003cstrong\u003eGel staining steps \u003c\/strong\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e1. Gel cleaning before staining \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlace the SDS-PAGE gel in a clean container, add 300 mL~400 mL purified water, shake and wash \u003c\/span\u003e\u003cspan\u003efor 5 minutes, repeat 3 times. If performing Native PAGE, rinse with purified water for 5 minutes. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNote\u003c\/strong\u003e: \u003c\/span\u003e\u003cspan\u003eSDS inhibits the binding of the staining solution to the protein, so be sure to rinse the gel with sufficient \u003c\/span\u003e\u003cspan\u003epurified water. If you use 200 mL purified water for cleaning, it is recommended to clean it for 10 minutes × 3 \u003c\/span\u003e\u003cspan\u003etimes. If the gel thickness is \u0026gt;1 mm or the concentration is \u0026gt;15%, it is recommended to clean it for 10 minutes × 3 \u003c\/span\u003e\u003cspan\u003etimes. If using large pieces of gel, please clean it with 5mL of purified water per square centimeter. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Staining \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eShake SpectBlue staining solution evenly before use. Discard the purified water and add an \u003c\/span\u003e\u003cspan\u003eappropriate volume of SpectBlue staining solution (appropriate to cover the gel). Place on a shaking \u003c\/span\u003e\u003cspan\u003etable for staining for 15 to 30 minutes. After the staining is completed, discard the staining solution \u003c\/span\u003e\u003cspan\u003eand add purified water to wash to remove the residual staining solution, and the results can be \u003c\/span\u003e\u003cspan\u003eobserved. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: \u003c\/span\u003e\u003cspan\u003eIf the protein content is low, the staining time can be appropriately extended. The actual staining time can \u003c\/span\u003e\u003cspan\u003ebe determined based on the degree of band visualization. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Decolorization \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAdd 200 mL of purified water for decolorization. The purified water can be replaced every 20 \u003c\/span\u003e\u003cspan\u003eminutes for rinsing. Repeat 3 to 4 times to obtain a gel with extremely low background. Soaking the \u003c\/span\u003e\u003cspan\u003estained gel in purified water overnight can also achieve a good background removal effect and \u003c\/span\u003e\u003cspan\u003eobtain clear protein bands. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: \u003c\/span\u003e\u003cspan\u003eMicrowave oven or water preheated to 50℃~60℃ can be used for decolorization to shorten the \u003c\/span\u003e\u003cspan\u003edecolorization time. If large gels are used, please wash with 5 mL of purified water per square centimeter. \u003c\/span\u003e\u003cspan\u003eDecolorization steps in microwave oven: add 100 mL purified water to each mini gel, after microwave for 30 \u003c\/span\u003e\u003cspan\u003eseconds, gently shake it on a shaker for 5 minutes. This step can be repeated 2-3 times. Preheated water: add 100 \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003emL of purified water preheated at 50℃ ~60℃ to a container with colloid and shake it gently for 5 minutes. This \u003c\/span\u003e\u003cspan\u003estep can be repeated 2-3 times to completely clean the background. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGel preservation after decolorization \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe gel can be placed in a sealed bag containing pure water and stored at 4℃ for several weeks. Do \u003c\/span\u003e\u003cspan\u003enot leave the gel at room temperature. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDecolorization followed by MS analysis \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePlease refer to the following steps and consult the MS instrument service personnel for details. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Cut the protein band to be analyzed with a tissue knife and destain it with 10%~30% ethanol or \u003c\/span\u003e\u003cspan\u003e20%~30% acetonitrile for 10 to 15 minutes. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Wash with purified water and perform MS analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. This product is a ready-to-use liquid. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. This product is only used for scientific research by professionals, for your safety and health, \u003c\/span\u003e\u003cspan\u003eplease wear a lab coat and disposable gloves.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\"\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at 2℃~8℃ ,valid for 12 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on room temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8019011_SpectBlue_Protein_Staining_Reagent-_-C0-A4.pdf?v=1717660060\" type=\"application\/pdf\" target=\"_blank\"\u003eSpectBlue Protein Staining Reagent\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8019011_SpectBlue_-MSDS.pdf?v=1717660060\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSpectBlue Protein Staining Reagent-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"250 mL","offer_id":44250580648117,"sku":"8019011","price":33.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8019011SpectBlue.png?v=1732883265"},{"product_id":"superlimit-ecl-chemiluminescent-substrate-dakewe","title":"SuperLimit ECL Chemiluminescent Substrate - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eModel \u0026amp; Size\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_15-54-59_600x600.png?v=1717660514\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSuperlimit ECL chemiluminescent substrate can be catalysed by horseradish peroxidase labeled on \u003c\/span\u003e\u003cspan\u003ethe secondary antibody to produce chemiluminescence reaction and sensitively detect the presence \u003c\/span\u003e\u003cspan\u003eof target protein. The product is developed based on a new generation of enhanced \u003c\/span\u003e\u003cspan\u003echemiluminescence substrate, and the composition is optimized. This product has low background \u003c\/span\u003e\u003cspan\u003eand good stability, and is ten times more sensitive than ordinary ECL reagent. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis product can be used for Western blot of HRP labeled antibody and nucleic acid hybridization \u003c\/span\u003e\u003cspan\u003edetection of HRP labeled probe. It can expose X-ray film, or directly carry out luminometer \u003c\/span\u003e\u003cspan\u003edetection or fluorescent CCD scanning. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThis product has extremely high sensitivity, can detect (10-100) FG antigen, and can use higher \u003c\/span\u003e\u003cspan\u003eantibody dilution ratio (1:2000-1:10000) to save antibody extremely; It can be operated under \u003c\/span\u003e\u003cspan\u003enatural light without entering the darkroom; It emits rapidly and lasts for a long time.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. This product is commonly used for Western Blot detection of HRP-labeled antibodies. Note the \u003c\/span\u003e\u003cspan\u003euse of HRP-labeled IgG or primary antibody-streptavidin-biotin-HRP. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Western Blot secondary antibody incubation. After the last wash of the membrane is completed, \u003c\/span\u003e\u003cspan\u003edrain it on filter paper (do not completely dry it). Mix equal volumes of A-Luminol Solution and \u003c\/span\u003e\u003cspan\u003eB-Peroxide Buffe and drop them evenly on the membrane (0.125 mL solution\/cm\u003c\/span\u003e\u003cspan\u003e2 \u003c\/span\u003e\u003cspan\u003emembrane), \u003c\/span\u003e\u003cspan\u003eincubate at room temperature 1-2 minuets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Use filter paper to absorb excess luminescent liquid, place the membrane on a clean and flat \u003c\/span\u003e\u003cspan\u003eplastic wrap, and detect it on a chemiluminescence image.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. For antibodies of different brands, it is recommended to optimize the amount of antibody for the \u003c\/span\u003e\u003cspan\u003efirst time to obtain the best effect. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. When preparing ECL working fluid, the pipette tip must be replaced when absorbing liquid A \u003c\/span\u003e\u003cspan\u003eand liquid B. Mutual contamination will cause the gradual failure of liquid A and liquid B. The \u003c\/span\u003e\u003cspan\u003eworking liquid should be prepared and used immediately. The sensitivity will decrease after being \u003c\/span\u003e\u003cspan\u003eleft at room temperature for several hours. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. After preparing the Superlimit ECL Chemiluminescent Substrate, please tighten the bottle cap. \u003c\/span\u003e\u003cspan\u003eDo not expose the kit to sunlight or strong light, otherwise it will become inactivated.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cspan\u003e4. The fluorescence of SuperLimit ECL Chemiluminescent Substrate will last for a long time, but \u003c\/span\u003e\u003cspan\u003ethe fluorescence will be strongest within 30 minutes after the reaction, and then the fluorescence \u003c\/span\u003e\u003cspan\u003ewill gradually weaken. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Metal oxide particles may cause granular spots on the membrane. Avoid using rusty scissors and \u003c\/span\u003e\u003cspan\u003etweezers. It is recommended to use plastic flat-tipped tweezers. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. Both Liquid A and Liquid B are harmful to the human body, please pay attention to protection \u003c\/span\u003e\u003cspan\u003ewhen using them. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7. \u003c\/span\u003e\u003cspan\u003eThis product is only used for scientific research by professionals, for your safety and health, \u003c\/span\u003e\u003cspan\u003eplease wear a lab coat and disposable gloves.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-31-33_600x600.png?v=1717655509\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStored at 2℃~8℃ protected from light, with a shelf life of 12 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIf not used for a long time, it can be stored at -25℃~-15℃. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on blue ice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8061011_SuperLimit_ECL_Chemiluminescent_Substrate-_-C0-A4.pdf?v=1717660773\" type=\"application\/pdf\" target=\"_blank\"\u003eSuperLimit ECL Chemiluminescent Substrate\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8061011_SuperLimit_ECL_-MSDS.pdf?v=1717660773\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cspan\u003eSuperLimit ECL Chemiluminescent Substrate-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c!----\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003esubstrates\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"100 mL\/Kit","offer_id":44250619871413,"sku":"8061011","price":480.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8061011_806101T_SuperLimit_ECL_Chemiluminescent_Substrate.png?v=1732884079"},{"product_id":"stripping-protocol-stripsure™-western-blot-stripping-buffer","title":"Western Blot Stripping Buffer (StripSure™)","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cmeta name=\"viewport\" content=\"width=device-width, initial-scale=1\"\u003e\u003cmeta name=\"description\" content=\"Mild, ready-to-use Western blot stripping buffer. Complete antibody removal in 20 minutes at room temperature — no heat, no harsh chemicals. PVDF, nitrocellulose \u0026amp; nylon. 500 mL.\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n    :root{--ink:#183042;--muted:#587080;--line:#dbe5e9;--tint:#f3f9f8;--accent:#0d776b;--warm:#f3bf4d}*{box-sizing:border-box}body{margin:0;background:#f5f7f8;color:var(--ink);font:16px\/1.6 Arial,sans-serif}.page{max-width:1020px;margin:32px auto;background:#fff;padding:54px 70px;box-shadow:0 5px 24px #18304218}.eyebrow{margin:0 0 10px;color:var(--accent);font-weight:700;letter-spacing:.08em;text-transform:uppercase;font-size:.78rem}h1{font-size:2.35rem;line-height:1.18;margin:0 0 15px}h2{font-size:1.5rem;line-height:1.28;margin:46px 0 17px;padding-top:8px}h3{font-size:1.12rem;margin:25px 0 8px}.lead{font-size:1.14rem;color:#29485a}.callout{margin:25px 0;padding:20px 23px;border-left:5px solid var(--accent);background:var(--tint)}.facts{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:28px 0}.fact{padding:17px;border:1px solid var(--line);border-radius:5px}.fact strong{display:block;color:var(--accent);font-size:1.25rem}.table-wrap{overflow-x:auto}table{border-collapse:collapse;width:100%;margin:16px 0 4px}th,td{border:1px solid var(--line);padding:12px 13px;text-align:left;vertical-align:top}th{background:#eaf5f3;font-weight:700}tr:nth-child(even) td{background:#fafcfc}li{margin:7px 0}.images{display:grid;grid-template-columns:repeat(2,1fr);gap:18px}.images figure{margin:0;border:1px solid var(--line);padding:10px}.images img{display:block;width:100%;height:auto}.images figcaption{font-size:.9rem;color:var(--muted);padding-top:8px}.note{color:var(--muted);font-size:.9rem}.download{display:inline-block;margin-top:28px;padding:11px 17px;background:var(--accent);color:white;text-decoration:none;border-radius:4px;font-weight:700}details{border-top:1px solid var(--line);padding:13px 0}summary{cursor:pointer;font-weight:700}@media(max-width:700px){.page{margin:0;padding:30px 22px}.facts,.images{grid-template-columns:1fr}h1{font-size:1.9rem}}\n  \u003c\/style\u003e\n\u003cp\u003e\u003cscript type=\"application\/ld+json\"\u003e{\"@context\":\"https:\/\/schema.org\",\"@type\":\"HowTo\",\"name\":\"Western Blot Stripping Protocol (20 Minutes, Room Temperature)\",\"totalTime\":\"PT20M\",\"step\":[{\"@type\":\"HowToStep\",\"name\":\"Initial wash\",\"text\":\"Rinse the membrane once with deionized water to remove residual detection reagents.\"},{\"@type\":\"HowToStep\",\"name\":\"Stripping\",\"text\":\"Gently mix ready-to-use StripSure without diluting. Completely immerse the membrane.\"},{\"@type\":\"HowToStep\",\"name\":\"Incubation\",\"text\":\"Incubate for 20 minutes at room temperature (20–25°C) with gentle agitation.\"},{\"@type\":\"HowToStep\",\"name\":\"Rinsing\",\"text\":\"Rinse three times with deionized water, or three times with TBST for sensitive applications.\"},{\"@type\":\"HowToStep\",\"name\":\"Re-equilibration\",\"text\":\"Equilibrate the membrane in TBST for 5 minutes.\"},{\"@type\":\"HowToStep\",\"name\":\"Re-probing\",\"text\":\"Proceed with the standard Western blot workflow.\"}]}\u003c\/script\u003e \u003cscript type=\"application\/ld+json\"\u003e{\"@context\":\"https:\/\/schema.org\",\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\":\"Question\",\"name\":\"How long does Western blot stripping take?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"StripSure requires a 20-minute incubation at room temperature with gentle agitation.\"}},{\"@type\":\"Question\",\"name\":\"Can you strip and re-probe a phospho-specific Western blot?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Yes. StripSure is a mild, room-temperature, non-reducing buffer designed for re-probing, including phospho-specific workflows.\"}},{\"@type\":\"Question\",\"name\":\"How many times can you strip the same membrane?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"A membrane can typically be stripped no more than two to three times; re-run the blot after that.\"}},{\"@type\":\"Question\",\"name\":\"Is StripSure a mild or harsh stripping buffer?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"StripSure is a mild stripping buffer: it works at room temperature without heat, beta-mercaptoethanol, or reducing agents.\"}},{\"@type\":\"Question\",\"name\":\"Does it work with nitrocellulose and PVDF?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Yes. StripSure is compatible with PVDF, nitrocellulose, and nylon membranes.\"}},{\"@type\":\"Question\",\"name\":\"Do I need to re-block after stripping?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Yes. After stripping and washing, re-block the membrane for at least one hour before re-probing.\"}},{\"@type\":\"Question\",\"name\":\"What is the difference between mild and harsh stripping buffers?\",\"acceptedAnswer\":{\"@type\":\"Answer\",\"text\":\"Harsh buffers use SDS and beta-mercaptoethanol at elevated temperatures, whereas mild buffers use low pH and mild detergent at room temperature to help preserve membrane-bound target protein.\"}}]}\u003c\/script\u003e\u003c\/p\u003e\n\u003cmain class=\"page\"\u003e\n\u003cp class=\"eyebrow\"\u003eBioFargo Inc. · Ready-to-use · 500 mL\u003c\/p\u003e\n\u003ch1\u003eWestern Blot Stripping Buffer (StripSure™)\u003c\/h1\u003e\n\u003cp class=\"lead\"\u003e\u003cstrong\u003eMild, ready-to-use antibody stripping buffer for complete removal in 20 minutes at room temperature—without heat or harsh chemicals.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStripSure™ efficiently removes primary and secondary antibodies from Western blot membranes while preserving membrane-bound target proteins for reliable re-probing. It is compatible with PVDF, nitrocellulose, and nylon membranes; simply immerse, incubate, rinse, and re-probe.\u003c\/p\u003e\n\u003cp\u003ePerformance testing confirmed removal of detectable signal from previously probed antibodies (anti-ATG7), while re-probing generated strong, specific signal with β-actin and phospho-STAT3 (Tyr705). Its gentle conditions support sensitive phospho-specific detection workflows.\u003c\/p\u003e\n\u003csection class=\"facts\"\u003e\n\u003cdiv class=\"fact\"\u003e\n\u003cstrong\u003e20 min\u003c\/strong\u003eRoom temperature protocol\u003c\/div\u003e\n\u003cdiv class=\"fact\"\u003e\n\u003cstrong\u003eMild\u003c\/strong\u003eNo heat or reducing agents\u003c\/div\u003e\n\u003cdiv class=\"fact\"\u003e\n\u003cstrong\u003e3 membranes\u003c\/strong\u003ePVDF, nitrocellulose \u0026amp; nylon\u003c\/div\u003e\n\u003c\/section\u003e\n\u003ch2\u003eKey Features \u0026amp; Benefits\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEfficient removal of primary and secondary antibodies\u003c\/li\u003e\n\u003cli\u003eCompatible with phospho-specific antibody re-probing\u003c\/li\u003e\n\u003cli\u003eHelps preserve membrane-bound target proteins across stripping cycles\u003c\/li\u003e\n\u003cli\u003eReady-to-use liquid formulation—do not dilute\u003c\/li\u003e\n\u003cli\u003e20-minute protocol at room temperature (20–25°C) with gentle agitation\u003c\/li\u003e\n\u003cli\u003eSuitable for chemiluminescent and fluorescent detection systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eMild vs. Harsh Stripping: Which Do You Need?\u003c\/h2\u003e\n\u003cp\u003eStripping buffers fall into two categories, and choosing the wrong one is a common reason a re-probe fails.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHarsh stripping\u003c\/strong\u003e uses SDS and β-mercaptoethanol at 50–70°C. It removes essentially any antibody, including high-affinity antibodies, but can also strip target protein from the membrane. Signal loss on a second probe can be measurable, particularly for low-abundance targets.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMild stripping\u003c\/strong\u003e uses low pH and mild detergent at room temperature. It removes antibody without displacing membrane-bound protein, supporting sequential probing that is quantitative rather than qualitative.\u003c\/p\u003e\n\u003cdiv class=\"callout\"\u003e\n\u003cstrong\u003eStripSure is a mild stripping buffer.\u003c\/strong\u003e It requires no heat, β-mercaptoethanol, or reducing agents: 20 minutes at room temperature. Use it when the second blot must be comparable to the first, when re-probing phospho-epitopes, or when a low-abundance target cannot tolerate signal loss.\u003c\/div\u003e\n\u003cp\u003eIf you are stripping a very high-affinity antibody and preserving the target is not important, a harsh protocol may still be faster. For other applications, mild stripping is the appropriate default.\u003c\/p\u003e\n\u003ch2\u003eWestern Blot Stripping Protocol (20 Minutes, Room Temperature)\u003c\/h2\u003e\n\u003ch3\u003eStep-by-Step\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cstrong\u003eInitial wash — \u003c\/strong\u003eRinse the membrane once with deionized water to remove residual detection reagents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStripping — \u003c\/strong\u003eGently mix StripSure™ until homogeneous (do not dilute), then completely immerse the membrane.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncubation — \u003c\/strong\u003eIncubate for 20 minutes at room temperature (20–25°C) with gentle agitation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRinsing — \u003c\/strong\u003eRinse three times with deionized water, or three times with TBST for sensitive applications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRe-equilibration — \u003c\/strong\u003eEquilibrate the membrane in TBST for 5 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRe-probing — \u003c\/strong\u003eProceed with primary antibody incubation, wash, secondary antibody incubation, wash, and detection.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp class=\"note\"\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e Keep the membrane fully submerged throughout stripping for consistent, even results.\u003c\/p\u003e\n\u003ch2\u003eStripping and Re-Probing Phospho-Specific Blots\u003c\/h2\u003e\n\u003cp\u003ePhospho-specific targets are particularly vulnerable to signal loss under heated or reducing stripping conditions. StripSure’s room-temperature, non-reducing mild stripping protocol is intended to retain membrane-bound protein for subsequent phospho-specific re-probing.\u003c\/p\u003e\n\u003ch2\u003eTroubleshooting: When Stripping Doesn’t Work\u003c\/h2\u003e\n\u003cdiv class=\"table-wrap\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eObservation\u003c\/th\u003e\n\u003cth\u003eMost likely cause\u003c\/th\u003e\n\u003cth\u003eWhat to do\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eOriginal signal is still visible\u003c\/td\u003e\n\u003ctd\u003eHigh-affinity antibody or membrane not fully immersed\u003c\/td\u003e\n\u003ctd\u003eRun another 20-minute cycle; ensure full immersion and continuous gentle agitation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSecond-probe signal is much weaker\u003c\/td\u003e\n\u003ctd\u003eTarget protein was eluted, often after a prior harsh protocol\u003c\/td\u003e\n\u003ctd\u003eUse a mild protocol; for low-abundance targets, consider re-running the blot.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHigh background on re-probe\u003c\/td\u003e\n\u003ctd\u003eInsufficient washing or residual stripping buffer\u003c\/td\u003e\n\u003ctd\u003eWash with TBST three times for 5 minutes each; re-block for at least 1 hour.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBlotchy or uneven signal\u003c\/td\u003e\n\u003ctd\u003eMembrane dried during incubation\u003c\/td\u003e\n\u003ctd\u003eKeep it wet throughout; move directly to washing after stripping.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhospho signal is lost\u003c\/td\u003e\n\u003ctd\u003eA heated or reducing protocol was used\u003c\/td\u003e\n\u003ctd\u003eUse a room-temperature, non-reducing mild protocol for phospho-epitopes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNothing works after two cycles\u003c\/td\u003e\n\u003ctd\u003eThe membrane reached its stripping limit\u003c\/td\u003e\n\u003ctd\u003eDo not strip the same membrane more than 2–3 times; re-run the blot.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eStripSure™ vs. Restore™ PLUS\u003c\/h2\u003e\n\u003cdiv class=\"table-wrap\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003cth\u003eStripSure™\u003c\/th\u003e\n\u003cth\u003eRestore™ PLUS (46430)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e500 mL price\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e$120\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e$189.65\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIncubation conditions\u003c\/td\u003e\n\u003ctd\u003e20 min, room temperature\u003c\/td\u003e\n\u003ctd\u003e5–15 min, room temperature; 37°C for high-affinity antibodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeat required\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003e37°C for difficult-to-strip antibodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMembrane compatibility\u003c\/td\u003e\n\u003ctd\u003ePVDF, nitrocellulose, \u003cstrong\u003enylon\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd\u003eNitrocellulose, PVDF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReady to use\u003c\/td\u003e\n\u003ctd\u003eYes; no dilution\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"note\"\u003e\u003cem\u003ePricing and specifications as published by the manufacturer, August 2026.\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"table-wrap\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003eStripSure™ Western Blot Stripping Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBrand\u003c\/th\u003e\n\u003ctd\u003eBioFargo Inc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eReady-to-use liquid (do not dilute)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible Membranes\u003c\/th\u003e\n\u003ctd\u003ePVDF, nitrocellulose, nylon\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Systems\u003c\/th\u003e\n\u003ctd\u003eChemiluminescent and fluorescent imaging\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIncubation\u003c\/th\u003e\n\u003ctd\u003e20 minutes at room temperature (20–25°C), gentle agitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Compatibility\u003c\/th\u003e\n\u003ctd\u003ePrimary and secondary antibodies, including phospho-specific antibodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRinse Options\u003c\/th\u003e\n\u003ctd\u003eDeionized water or TBST (recommended for sensitive applications)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize \/ Volume\u003c\/th\u003e\n\u003ctd\u003e500 mL bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003e1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eValidation Data\u003c\/h2\u003e\n\u003cp\u003eStripSure™ was validated for antibody removal using anti-ATG7 and for re-probing using β-actin and phospho-STAT3 (Tyr705).\u003c\/p\u003e\n\u003cdiv class=\"images\"\u003e\n\u003cfigure\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Screenshot_2026-08-17_at_11.50.05_AM.png?v=1786985452\" alt=\"Western blot membrane before stripping with StripSure\"\u003e\n\u003cfigcaption\u003eWestern blot validation data\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003cfigure\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Screenshot_2026-08-17_at_11.50.16_AM.png?v=1786985452\" alt=\"Western blot membrane after StripSure stripping and re-probing with anti-phospho-STAT3\"\u003e\n\u003cfigcaption\u003eStripping and re-probing validation data\u003c\/figcaption\u003e\n\u003c\/figure\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does Western blot stripping take?\u003c\/summary\u003e\nStripSure requires a 20-minute incubation at room temperature with gentle agitation.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan you strip and re-probe a phospho-specific Western blot?\u003c\/summary\u003e\nYes. StripSure is a mild, room-temperature, non-reducing buffer designed for re-probing, including phospho-specific workflows.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow many times can you strip the same membrane?\u003c\/summary\u003e\nTypically no more than two to three times. Re-run the blot after that.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs StripSure a mild or harsh stripping buffer?\u003c\/summary\u003e\nStripSure is a mild stripping buffer; it works at room temperature without heat, β-mercaptoethanol, or reducing agents.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes it work with nitrocellulose and PVDF?\u003c\/summary\u003e\nYes. It is compatible with PVDF, nitrocellulose, and nylon membranes.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDo I need to re-block after stripping?\u003c\/summary\u003e\nYes. After stripping and washing, re-block the membrane for at least one hour before re-probing.\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between mild and harsh stripping buffers?\u003c\/summary\u003e\nHarsh buffers use SDS and β-mercaptoethanol at elevated temperatures. Mild buffers use low pH and mild detergent at room temperature to help preserve membrane-bound protein.\u003c\/details\u003e\n\u003ca class=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/StripSure_-_BioFargo_Inc.-072126.docx?v=1786985291\"\u003eDownload Product User Manual\u003c\/a\u003e\u003c\/main\u003e","brand":"Biofargo","offers":[{"title":"Default Title","offer_id":52979990364341,"sku":"BF-SS-500","price":120.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20260817-114118.jpg?v=1786984894"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/Western_Blot_Buffers.png?v=1741787869","url":"https:\/\/biofargo.com\/collections\/western-blot-buffers.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}