{"title":"Standard PCR Reagents","description":"","products":[{"product_id":"hieff-clone-universal-one-step-cloning-kit","title":"Hieff Clone™ Universal II One Step Cloning Kit","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eHieff Clone\u003csup\u003e™\u003c\/sup\u003e Universal II One Step Cloning Kit is a new generation homologous recombinant cloning kit. The carefully optimized 2nd generation 2× Hieff Clone\u003csup\u003e™\u003c\/sup\u003e Universal II Enzyme Premix combines the recombinant enzyme and the buffer required for the recombinant reaction with the addition of a unique recombinant enhancer to significantly improve the efficiency of recombinant cloning.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit can be directed to clone PCR products to any site of any vector, compatible with unpurified PCR products, directly recovered PCR products, low concentration of rubber recovered products, this product can reassemble the homologous arm GC content of 30%-70% of the joint fragment. The vector was completely linearized, and homologous sequences of the end of the linearized vector of 15-25 bp were introduced into the 5 'end of the positive and reverse PCR primers of the inserted fragment, so that the 5' and 3 'ends of the PCR products of the inserted fragment had exactly the same sequence corresponding to the two ends of the linearized vector, respectively. Under the action of recombinant enzyme, the recombinant reaction of PCR product and linearized vector can be completed in as little as 5 min at 50℃. The positive rate of cloning can reach more than 95%.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSimple: Seamlessly assemble and clone up to six DNA fragments in a single reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFlexible: Design guidelines allow assembly into any vector of your choice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient: Efficient for ligation of one to six fragments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eRapid Cloning: It allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 5–50 minute isothermal reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eDirected Cloning; Site-Directed Mutagenesis.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 76px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 30.7573%; text-align: center; height: 19px;\"\u003eCloning Process\u003c\/td\u003e\n\u003ctd style=\"width: 69.0881%; text-align: center; height: 19px;\"\u003eSeamless cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 30.7573%; text-align: center; height: 19px;\"\u003eControl\u003c\/td\u003e\n\u003ctd style=\"width: 69.0881%; text-align: center; height: 19px;\"\u003ePositive control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 30.7573%; text-align: center; height: 19px;\"\u003eSegments\u003c\/td\u003e\n\u003ctd style=\"width: 69.0881%; text-align: center; height: 19px;\"\u003eUp to 6 fragments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 30.7573%; text-align: center; height: 19px;\"\u003eProduct Type\u003c\/td\u003e\n\u003ctd style=\"width: 69.0881%; text-align: center; height: 19px;\"\u003eSeamless Cloning and Assembly Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.9054%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 37.1621%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2027%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923ES05\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.5406%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923ES20\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.1892%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923ES50\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.9054%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 37.1621%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e2 \u003cspan\u003e×\u003c\/span\u003e Hieff Clone\u003csup\u003eTM\u003c\/sup\u003e\u003csup\u003e \u003c\/sup\u003eUniversal II Enzyme Premix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2027%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e50 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.5406%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.1892%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.9054%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 37.1621%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e500 bp control insert (25 ng\/\u003cspan\u003eμL\u003c\/span\u003e)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2027%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.5406%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.1892%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 17.9054%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e10923-C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 37.1621%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003epUC 19 control vector, linearized (50 ng\/\u003cspan\u003eμL\u003c\/span\u003e)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2027%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.5406%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.1892%; text-align: center;\"\u003e\n\u003cp style=\"font-weight: inherit;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 class=\"H3_backgro\"\u003eFigures\u003c\/h2\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/109231_600x600_05e72a8e-e177-4dd1-b0b5-46c0a13891a8.webp?v=1728522315\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 1. A total of 10 ng low-input single fragment was cloned into a 10 kb plasmid. The results showed that 10923ES had better performance than competing products, with high connection efficiency, more colonies and a positive rate of 100%. A-B: Colony plate. The molar ratio of carrier (10 kb) to insert fragment (1 kb) was 1:2. C: Electrophoresis analysis of the insertion fragment, M: YEASEN 10505ES. Plasmid input 40 ng\/6 μL, 50°C, 15 mins.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/109232_600x600_d8ec1c24-735b-406c-8125-2323fd74fb77.webp?v=1728522380\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003eFigure 2. Six fragments (a total length of 7.6 kb) was cloned into a 11.6 kb plasmid. The results showed that 10923ES had better performance than competing products, with more colonies and positive rate of 100%. A-B: Colony plate. The molar ratio of carrier (11.6 kb) to insert fragments was 1:2. C: Electrophoresis analysis of the insertion fragment, M: YEASEN 10505ES. Plasmid input：200 ng\/20 μL, 50°C, 50 mins.\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/109233_600x600_d493a7d2-b473-4e43-b953-fca3b4a51334.webp?v=1728522440\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp class=\"H3_backgro\"\u003eFigure 3. A 4.5kb fragment was cloned into a 21 kb plasmid. A: Colony plate. The molar ratio of plasmid to insert fragment was 1:2. B: Electrophoresis analysis of the insertion fragment, M: YEASEN 10505ES. Plasmid input：200 ng\/20 μL, 50°C, 40 mins.\u003c\/p\u003e\n\u003ch2 class=\"H3_backgro\"\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for 1 years.\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasenbio","offers":[{"title":"5T","offer_id":43046797246645,"sku":"10923ES05","price":35.0,"currency_code":"USD","in_stock":true},{"title":"20T","offer_id":43046797279413,"sku":"10923ES20","price":201.0,"currency_code":"USD","in_stock":true},{"title":"50T","offer_id":43046797312181,"sku":"10923ES50","price":338.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HieffClone_UniversalIIOneStepCloningKit.jpg?v=1765186646"},{"product_id":"super-phi29-dna-polymerase-kleanab","title":"Super Phi29 DNA Polymerase-KleanAB","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Table SOP (Embedded)\n     - Wrapper scroll + table min-width\n     - Strong selector weight via .biofargo-wrap prefix\n     - 2-column table default: 640px\n  ========================== *\/\n  .biofargo-wrap .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n    margin-top:15px;\n  }\n  .biofargo-wrap table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:640px; \/* 2-column table *\/\n    background:#fff;\n  }\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td{\n    border:1px solid #e9ecef;\n    padding:15px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  \/* If you ever add a header row later *\/\n  .biofargo-wrap table.biofargo-table thead th{\n    background:#f2f2f2;\n    color:#000;\n    font-weight:700;\n    text-align:left;\n  }\n  \/* Parameter\/Item column *\/\n  .biofargo-wrap table.biofargo-table tbody td:first-child{\n    background:#f8f9fa;\n    font-weight:600;\n    width:30%;\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Description\n    \u003c\/h2\u003e\n    \u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n      Super Phi29 DNA Polymerase is derived from recombinant E. coli carrying the Bacillus subtilis phage Phi29 gene, which was expressed by E. coli and isolated and purified multiple times. Phi29 DNA polymerase has high continuous synthesis capacity and strand replacement activity, and can continuously synthesize up to 70 kb of DNA fragments to achieve efficient isothermal DNA amplification. It has 3'-5' exonuclease (proofreading) activity that preferentially acts on single-stranded DNA or RNA, ensuring high fidelity of amplification reactions, and is commonly used in vitro preparation and genome-wide synthesis of plasmids. It has higher amplification efficiency and sensitivity compared to wild-type Phi29 DNA polymerase, and DNA synthesis can be performed continuously at 42°C (whereas wild-type Phi29 DNA polymerase has low reactivity at this temperature).\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Specification\n    \u003c\/h2\u003e\n\n    \u003c!-- SOP-optimized responsive table wrapper --\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eSuper Phi29 DNA Polymerase\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eReaction Buffer\u003c\/td\u003e\n            \u003ctd\u003e10X Super Phi29 Buffer\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eUnit Definition\u003c\/td\u003e\n            \u003ctd\u003e1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 minutes at 30°C.\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eThermal Inactivation\u003c\/td\u003e\n            \u003ctd\u003eHeat at 65 °C for 10 min to lose activity.\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eConcentration\u003c\/td\u003e\n            \u003ctd\u003e10 U\/μl\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n            \u003ctd style=\"color: #666; font-size: 13px;\"\u003e50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v\/v) NP-40, 0.5% (v\/v) Tween 20, 50% (v\/v) glycerol.\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Features\n    \u003c\/h2\u003e\n    \u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eHighest processability and strand displacement activity of any known DNA polymerase – more than 70 kb of DNA fragments can be synthesized;\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eHigh-precision DNA synthesis;\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eExtremely high yields of amplified DNA can be obtained even from very small amounts of template;\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 0; padding-left: 5px;\"\u003eAmplification products can be used directly for downstream applications (PCR, restriction digestion, SNP genotyping, etc.).\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Applications\n    \u003c\/h2\u003e\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eRolling Ring Amplification (RCA)\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eMultiple Displacement Amplification (MDA)\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eDNA amplification for SNP and STR detection\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eWhole genome amplification of single cells\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003ePathogenic microorganisms and metagenomes\u003c\/li\u003e\n      \u003c\/ul\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eDNA amplification in blood spot samples\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eDNA template preparation for sequencing\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eRNA and protein primers DNA amplification\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eUnbiased amplification of the whole genome\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eCell-free cloning of lethal DNA\u003c\/li\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px;\"\u003e\n    \u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 40px;\"\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eComponents\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background-color: #fff; font-size: 14px;\"\u003e\n          Super Phi29 DNA Polymerase,（10 U\/μl）\u003cbr\u003e\n          10X Super Phi29 Buffer\u003cbr\u003e\n          100 mM DTT\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eStorage\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background-color: #fff5f5; color: #c53030; font-weight: bold; font-size: 18px; border-radius: 4px;\"\u003e\n          -20°C\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"250 U","offer_id":43839043272885,"sku":"P310142-0250","price":56.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310142_1.jpg?v=1732884055"},{"product_id":"2x-taq-pcr-mix-kleanab","title":"2X Taq PCR Mix-KleanAB","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Table SOP (Embedded)\n     - Wrapper scroll + table min-width\n     - Strong selector weight via .biofargo-wrap prefix\n     - 2-column table default: 640px\n  ========================== *\/\n  .biofargo-wrap .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n    margin-top:15px;\n  }\n  .biofargo-wrap table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:640px; \/* 2-column table *\/\n    background:#fff;\n  }\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td{\n    border:1px solid #e9ecef;\n    padding:15px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  \/* If you ever add a header row later *\/\n  .biofargo-wrap table.biofargo-table thead th{\n    background:#f2f2f2;\n    color:#000;\n    font-weight:700;\n    text-align:left;\n  }\n  \/* Parameter\/Item column *\/\n  .biofargo-wrap table.biofargo-table tbody td:first-child{\n    background:#f8f9fa;\n    font-weight:600;\n    width:30%;\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Description\n    \u003c\/h2\u003e\n    \u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n      This reagent kit is fast and convenient to use, and can be used for the rapid establishment of ordinary PCR systems and other PCR related experiments. 2X SanTaq PCR Mix includes MgCl2, dNTP, Taq DNA Polymerase, PCR buffer, loading, and PCR enhancer. The optimized PCR Mix enables most PCR reactions to proceed well. The preparation of the PCR reaction system can be completed within a few minutes by adding primers, template DNA, and an appropriate amount of deionized water.\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Specification\n    \u003c\/h2\u003e\n\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003e2X Taq PCR Mix\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eConcentration\u003c\/td\u003e\n            \u003ctd\u003e25 μg \/ 500 μl\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eColor\u003c\/td\u003e\n            \u003ctd\u003eBlue\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Features\n    \u003c\/h2\u003e\n    \u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThe product contains bromophenol blue dye, and the PCR product can be directly subjected to electrophoresis.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThis product contains excellent stabilizers and can be stored for 3 months at 4 ℃.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 0; padding-left: 5px;\"\u003eThe PCR product has a 3 '- dA protruding end and can be easily cloned into the T vector.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Applications\n    \u003c\/h2\u003e\n\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 0;\"\u003eConventional PCR\u003c\/li\u003e\n      \u003c\/ul\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px;\"\u003e\n    \u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 40px;\"\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eComponents\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background-color: #fff; font-size: 14px;\"\u003e\n          2X SanTaq PCR Mix (with blue dye)\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eStorage\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background-color: #fff5f5; color: #c53030; font-weight: bold; font-size: 18px; border-radius: 4px;\"\u003e\n          -20°C\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Sangon","offers":[{"title":"1ml","offer_id":43839167398069,"sku":"P310149-0100","price":8.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310149_1.jpg?v=1732864843"},{"product_id":"2-phanta-flash-master-mix-dye-plus","title":"2 x Phanta Flash Master Mix (Dye Plus)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e2 × Phanta Flash Master Mix is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec\/kb) while maintaining high fidelity and yield. The amplification error rate of Phanta Flash Super-Fidelity DNA Polymerase is 81-fold lower than that of conventional \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer\/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This product contains tracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHigh fidelity:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e ~81X higher than \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlash amplification: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eExtension speed up to 5 sec\/kb\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eRobust performance: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuperior specificity, sensitivity, and yields\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBroad Compatibility: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuitable for complex samples \u0026amp; GC-rich systems\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eP520-01\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-02\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-03\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e2 × Phanta Flash Master Mix (Dye Plus)\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e5×1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e15×1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExperimental Cases\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2 × Phanta Flash Master Mix (Vazyme#P510), 2 × Phanta Flash Master Mix (Dye Plus) (Vazyme#P520) and other commercially available brands of high-fidelity polymerases were used to perform PCR amplification. The target fragment of 353 bp and 873 bp were amplified at an extension time of 1 sec\/kb, 7140 bp fragment was amplified at an extension time of 4 sec\/kb and 5 sec\/kb, respectively. The results show that all the fragments could be successfully amplified by P510 and P520.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1.png?v=1765791826\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAmplification of fragments ≤10 kb can be achieved within 1 h!\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_2cd7730b-c2f8-4911-b129-4685a4c7e4f7.png?v=1765791869\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Specificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eVazyme#P510, Vazyme#P520 and other commercially available brands of high-fidelity polymerases were used to amplify different length of fragments in accordance to the recommended systems and procedures of respective instructions. The results show that P510 and P520 have higher amplification specificity than other brands of high-fidelity polymerases.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_1d7164bb-8d83-4cfa-8c65-f27b25d4f9f7.png?v=1765791908\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at -30 ~ -15°C and transport at ≤0°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ1: Does the tracking dye in 2 × Phanta Flash Master Mix (Dye Plus) interfere with downstream applications?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA1: The tracking dye (which consists of Bromophenol Blue) is compatible with downstream applications such as fluorescent automated DNA sequencing, ligation, and restriction digestion. For applications that require analysis of PCR products by absorbance or fluorescence excitation, we recommend using colorless versions of the products Phanta Flash Super-Fidelity DNA Polymerase or 2 × Phanta Flash Master Mix or purifying the PCR products prior to analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ2: Can I perform restriction digestion in the Phanta Flash reaction buffer?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA2: Phanta Flash reaction buffer is compatible with restriction digestion directly after PCR. Note that Phanta Flash Super-Fidelity DNA Polymerase is not inactivated during PCR, thus purification of PCR products before restriction digestion is recommended if intact 5'- or 3'- overhangs are needed for cloning.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ3: Can I amplify GC-rich targets with Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA3: Phanta Flash Super-Fidelity DNA Polymerase can amplify targets with high GC content (up to 83% GC) without any additional DNA melting agents. In cases of extremely GC-rich targets (\u0026gt;83% GC), addition of DMSO to final concentration of 5% is recommended.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ4: What are your recommendations when working with long amplicons using Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA4: Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.3 µM may also improve the results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ5: What are your recommendations when calculating annealing temperatures using Phanta Flash Super-Fidelity DNA Polymerase?\u003cbr\u003e\u003c\/strong\u003eA5: Approximately 5°C above Tm of primers, but no more than 68°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Manual_P520_V23.1.pdf?v=1716346848\" type=\"application\/pdf\" target=\"_blank\"\u003eManual_P520 V23.1\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003ePurchase Help\u003c\/h2\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/b8a2a61f1ed241a5ada61e12cf731c35.png?v=1765792300\"\u003e\u003c\/p\u003e\n\u003c!----\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003epcr buffer,master mix\u003c\/div\u003e","brand":"Vazyme","offers":[{"title":"15×1ml","offer_id":44176146596021,"sku":"P520-03","price":897.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml","offer_id":44176146628789,"sku":"P520-02","price":320.0,"currency_code":"USD","in_stock":true},{"title":"1ml","offer_id":44176146661557,"sku":"P520-01","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1780083306473254913.jpg?v=1732864515"},{"product_id":"taq-dna-polymerase","title":"Taq DNA Polymerase","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Description --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cdiv class=\"desc-box\"\u003e\n\u003cp style=\"margin:0;\"\u003e\nThis product was purified from a plasmid cloned with a gene encoding Thermus aquaticus DNA polymerase and induced expression by E. coli. Taq DNA polymerase does not have 3'→5' exonuclease correction activity, but has very low 5'→3' exonuclease activity. Recombinant Taq DNA polymerase is the recommended choice for most PCR reactions, with a half-life of \u0026gt; 40 min at 95°C. The mutation rate of each circulating Taq DNA polymerase \u0026lt; 2.2×10-5. The above-mentioned Taq DNA polymerase is free of contamination by foreign nucleases and bacterial DNA, and has high amplification efficiency and good stability, making it suitable for routine PCR amplification.\n\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table w-640\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e1 KU\/5 KU\/200 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eTaq DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003eTaq DNA polymerase (5 U\/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 .\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd\u003e10X PCR Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eOne unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e5 U\/μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e20 mM Tris-HCl (PH 8.0) ；0.1 mM EDTA,；1 mM DTT；0.5% Tween 20；100 mM KCl；0.5% NP-40；50% glycerin.\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003eTaq DNA polymerase (5 U\/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 .\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Features --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin:0;\"\u003eThere is no contamination of foreign nuclease and bacterial DNA, and the amplification efficiency is high and the stability is good.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin:0;\"\u003eConventional PCR amplification, RT-PCR, and direct TA cloning of the product.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin:0;\"\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n  \u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003ep310138-200 u, p310138-200, sku p310138-200u, sku p310138, sku p310138200u, skup310138-200u\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"200U","offer_id":52400871211189,"sku":"P310138-200U","price":5.0,"currency_code":"USD","in_stock":true},{"title":"1KU","offer_id":52400871145653,"sku":"P310138-1KU","price":18.0,"currency_code":"USD","in_stock":true},{"title":"5KU","offer_id":52400871178421,"sku":"P310138-5KU","price":71.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_taqdnapolymerase.png?v=1751472347"},{"product_id":"pfu-dna-polymerase","title":"Pfu DNA Polymerase","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  \n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cdiv class=\"desc-box\"\u003e\n\u003cp style=\"margin: 0;\"\u003eThis product is a high-fidelity high-temperature resistant DNA polymerase isolated from the pyrophilus furiosis, with a molecular weight of 90 kDa, with 3'→5' exonuclease activity, no 5'→3' exonuclease activity, and its amplification product is blunt-end without protruding A. The elongation velocity of DNA polymerization was 600 bp\/min, and the PCR product was blunt-end. Other high-temperature DNA polymerases, such as Vent, DeepVent, Tli, UlTma, etc., have correction functions, but Pfu has a very low error rate in amplifying DNA among all DNA polymerases that have been discovered so far.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003e \u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eThe following catalog numbers have been updated for improved product management:\u003cbr\u003eP310139-0100 → P310139-100U\u003cbr\u003eP310139-0200 → P310139-200U\u003cbr\u003eP310139-0500 → P310139-500U\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eP310139-5000 → P310139-5KU\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table w-640\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 40%;\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e5 KU\/100 U\/200 U\/500 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003ePfu DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003ePfu DNA polymerase (5 U\/μL), 10X PCR Buffer (20 mM MgSO4).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd\u003e10X PCR Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitates at 74°C for 30 min was defined as 1 activity unit (U).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e5 U\/μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e20 mM Tris-HCl (pH 8.2); 0.1 mM EDTA; 0.1% Tween 20; 0.1% NP-40; 1 mM DTT; 100 mM KCl; 50% glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003ePfu DNA polymerase (5 U\/μL), 10X PCR Buffer (20 mM MgSO4).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Features --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe fidelity effect is 8 times higher than that of Taq enzyme.\u003c\/li\u003e\n\u003cli\u003eIt is highly thermostable, and the viability of incubation at 95°C for 1 h still maintains more than 90%.\u003c\/li\u003e\n\u003cli\u003eProduces blunt-ended PCR products that bind modified nucleotides (e.g., biotin, digoxigenin, fluorescently labeled nucleotides).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin: 0;\"\u003eConventional PCR, high-fidelity PCR, site-directed mutagenesis, clonal expression.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin: 0;\"\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"100U","offer_id":52400871506101,"sku":"P310139-100U","price":6.0,"currency_code":"USD","in_stock":true},{"title":"200U","offer_id":52400871538869,"sku":"P310139-200U","price":8.0,"currency_code":"USD","in_stock":true},{"title":"500U","offer_id":52400871571637,"sku":"P310139-500U","price":14.0,"currency_code":"USD","in_stock":true},{"title":"5KU","offer_id":52400871473333,"sku":"P310139-5KU","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_pfudnapolymerase.png?v=1751472348"},{"product_id":"hotstart-taq-dna-polymerase-chemical-mod","title":"HotStart Taq DNA Polymerase(chemical mod","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Description --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cdiv class=\"desc-box\"\u003e\n\u003cp style=\"margin:0;\"\u003e\nThis product is a chemically modified Taq DNA polymerase for use with HotStart PCR, which inhibits the activity of Taq enzyme due to its chemical modification. Taq DNA polymerase activity was completely inhibited at room temperature, but inhibition failed at 95°C for 10 minutes, Taq enzyme activity was restored, and DNA could be polymerized, which inhibited non-specific amplification due to non-specific primer annealing or primer dimers at low temperatures. In addition, the product did not detect 3' to 5' school exonuclease activity and can be used for qPCR detection.\n\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table w-640\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e1 KU\/200 U\/500 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eHotStart Taq DNA Polymerase（chemical mod\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003eHotStart Taq DNA polymerase (5U\/ul), 5X PCR buffer (containing 10 mM MgCl2).\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd\u003e10X PCR Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eUnit Definition\u003c\/td\u003e\n\u003ctd\u003eThe amount of enzyme required to precipitate 10 nmol of dNTPs into acid-insoluble precipitate at 75°C for 30 min was defined as 1 activity unit (U).\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e5 U\/μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003eHotStart Taq DNA polymerase (5U\/ul), 5X PCR buffer (containing 10 mM MgCl2).\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin:0;\"\u003e\nPCR amplification of complex template DNA, PCR amplification of complex template cDNA (RT-PCR), sequencing of single- or double-stranded DNA, site-directed mutagenesis, real-time PCR.\n\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"sec\"\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"card\"\u003e\n\u003cp style=\"margin:0;\"\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"1KU","offer_id":52400872095925,"sku":"P310140-1KU","price":126.0,"currency_code":"USD","in_stock":true},{"title":"200U","offer_id":52400872128693,"sku":"P310140-200U","price":39.0,"currency_code":"USD","in_stock":true},{"title":"500U","offer_id":52400872161461,"sku":"P310140-500U","price":74.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_hotstarttaqdnapolymerase-2.png?v=1751472361"},{"product_id":"phi29-dna-polymerase","title":"Phi29 DNA Polymerase","description":"\u003ch2 class=\"MsoNormal\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis product is derived from recombinant E. coli carrying Bacillus subtilis phage phi29 clone gene 2, which was expressed by E. coli and isolated and purified multiple times. Phi29 is a DNA polymerase with high sequential synthesis capacity and strand displacement for efficient isothermal DNA amplification with 3'–5' exonuclease (proofreading) activity.\u003c\/p\u003e\n\u003cp\u003eThe catalog number has been updated from P310141-0001 to P310141-1KU. \u003c\/p\u003e\n\u003ch2 class=\"MsoNormal\"\u003eSpecification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%; height: 171px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eFull name\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003ePhi29 DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eComposition\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003ePhi29 DNA Polymerase (10 U\/μL), 10 × Phi29 Buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e10X phi29 Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 min at 30°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eThermal Inactivation\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eHeat at 60°C for 10 min to lose activity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e10 U\/μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v\/v) Nonidet P40, 0.5% (v\/v) Tween 20, 50% (v\/v) glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003ePhi29 DNA Polymerase (10 U\/μL), 10 × Phi29 Buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-precision DNA synthesis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eExtremely high amplified DNA yield can be obtained even from a very small amount of template\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe amplification products can be directly used in downstream applications (PCR, restriction enzyme digestion, SNP genotyping, etc.).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRoutine PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh throughput PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMultiplex PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGenotyping\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRolling circle amplification (RCA)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"MsoNormal\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1KU","offer_id":52400872259765,"sku":"P310141-1KU","price":147.0,"currency_code":"USD","in_stock":true},{"title":"5*1KU","offer_id":52400977182901,"sku":"P310141-5KU","price":664.0,"currency_code":"USD","in_stock":true},{"title":"250U","offer_id":52400872325301,"sku":"P310141-250U","price":46.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_phi29dnapolymerase.webp?v=1751472349"},{"product_id":"super-phi29-dna-polymerase","title":"Super Phi29 DNA Polymerase","description":"\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003ePackage\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e1 KU\/5 KU\/250 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eFull name\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eSuper Phi29 DNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e1. Rolling Ring Amplification (RCA); 2. Multiple Displacement Amplification (MDA); 3. DNA amplification for SNP and STR detection; 4. Whole genome amplification of single cells, pathogenic microorganisms, and metagenomes; 5. DNA amplification in blood spot samples from filter paper; 6. DNA template preparation for sequencing; 7. RNA and protein primers DNA amplification; 8. Unbiased amplification of the whole genome; 9. Cell-free cloning of lethal DNA; 10. High-precision DNA synthesis.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eSummary\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eSuper Phi29 DNA Polymerase is derived from recombinant E. coli carrying the Bacillus subtilis phage Phi29 gene, which was expressed by E. coli and isolated and purified multiple times. Phi29 DNA polymerase has high continuous synthesis capacity and strand replacement activity, and can continuously synthesize up to 70 kb of DNA fragments to achieve efficient isothermal DNA amplification. It has 3'-5' exonuclease (proofreading) activity that preferentially acts on single-stranded DNA or RNA, ensuring high fidelity of amplification reactions, and is commonly used in vitro preparation and genome-wide synthesis of plasmids. It has higher amplification efficiency and sensitivity compared to wild-type Phi29 DNA polymerase, and DNA synthesis can be performed continuously at 42°C (whereas wild-type Phi29 DNA polymerase has low reactivity at this temperature).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eFeatures\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e1. Highest processability and strand displacement activity of any known DNA polymerase – more than 70 kb of DNA fragments can be synthesized; 2. High-precision DNA synthesis; 3. Extremely high yields of amplified DNA can be obtained even from very small amounts of template; 4. Amplification products can be used directly for downstream applications (PCR, restriction digestion, SNP genotyping, etc.).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eComposition\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eSuper Phi29 DNA Polymerase (10 U\/μl), 10X Super Phi29 Buffer, 100 mM DTT.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e10X Super Phi29 Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 minutes at 30°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eThermal Inactivation\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eHeat at 65 °C for 10 min to inactivate the enzyme.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e10 U\/μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v\/v) NP-40, 0.5% (v\/v) Tween 20, 50% (v\/v) glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003eSuper Phi29 DNA Polymerase,（10 U\/μl） 10X Super Phi29 Buffer 100 mM DTT.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1KU","offer_id":52400872390837,"sku":"P310142-1KU","price":192.0,"currency_code":"USD","in_stock":true},{"title":"5KU","offer_id":52400872423605,"sku":"P310142-5KU","price":786.0,"currency_code":"USD","in_stock":true},{"title":"250U","offer_id":52400872456373,"sku":"P310142-250U","price":56.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_phi29dnapolymerase.webp?v=1751472349"},{"product_id":"2x-taq-pcr-mix","title":"2X Taq PCR Master Mix (with Dye)","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n  margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n  background:#f9f9f9;\n  padding:18px;\n  border-radius:10px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\n\/* =========================\n   Table Style\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n\/* =========================\n   Document Button\n========================== *\/\n\n.biofargo-doc-btn{\n  display:inline-block;\n  padding:10px 18px;\n  background:#0056b3;\n  color:#fff !important;\n  border-radius:6px;\n  text-decoration:none;\n  font-weight:600;\n}\n\n.biofargo-doc-btn:hover{\n  background:#003f85;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Background --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eBackground\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\"\u003e\n\n\u003cp\u003eThe 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays.\u003c\/p\u003e\n\n\u003cp\u003eThe added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eItem\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003e2X Taq PCR Master Mix (with Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage Size\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eConventional PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e2X SanTaq PCR Mix (with bromophenol blue dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eTaq Polymerase Source\u003c\/td\u003e\n\u003ctd\u003eRecombinant, E. coli Expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e25 μg \/ 500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReady-to-Use 2X Formulation\u003c\/strong\u003e\u003cbr\u003e\nPre-mixed with Taq DNA polymerase, MgCl₂, dNTPs, PCR buffer, and enhancers for fast PCR setup.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eDirect Gel Loading\u003c\/strong\u003e\u003cbr\u003e\nContains bromophenol blue dye, enabling PCR products to be loaded directly onto agarose gels.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReliable Amplification Performance\u003c\/strong\u003e\u003cbr\u003e\nOptimized buffer system supports stable and efficient amplification in routine PCR assays.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eTA Cloning Compatible\u003c\/strong\u003e\u003cbr\u003e\nGenerates PCR products with 3′-dA overhangs, facilitating efficient ligation into T-vectors.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eEnhanced Stability\u003c\/strong\u003e\u003cbr\u003e\nFormulated with stabilizers; can be stored at 4 °C for up to 3 months and long-term at −20 °C.\n\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eConventional PCR amplification\u003c\/li\u003e\n\u003cli\u003eGene cloning and insert preparation\u003c\/li\u003e\n\u003cli\u003eGenotyping and mutation analysis\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for DNA sequencing\u003c\/li\u003e\n\u003cli\u003eRoutine molecular biology research workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\" style=\"background:#fff;\"\u003e\n\u003cp\u003eStore the 2X master mix at -20°C.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Documents --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n\n\u003ca class=\"biofargo-doc-btn\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2X_Taq_PCR_Master_Mix_with_Dye.pdf?v=1766022843\" target=\"_blank\"\u003e\n2X Taq PCR Master Mix (with Dye)\n\u003c\/a\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003emaster mix\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"1ml","offer_id":52400976887989,"sku":"P310149-1ml","price":21.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_taqpcrmix.jpg?v=1751472348"},{"product_id":"dntp-mixture-10mm-biofargo","title":"dNTP mixture (10mM)","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eOur dNTPs are analytically tested to ensure a purity of \u0026gt;99% by HPLC, guaranteeing optimal performance in demanding PCR applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eParameter\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e ≥ 99% (HPLC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNumber of Containers\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eFreezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e12352208\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNucleic Acid Bases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eDG\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e(-15 to -20)℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eStock\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUsually in Stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Summary --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSummary\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border: 1px solid #eee;\"\u003e\n\u003cp\u003eDNase, RNase: None detected.\u003c\/p\u003e\n\u003cp\u003eSuitable for use in the Polymerase Chain Reaction (PCR).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- PCR Suitability --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePCR Suitability\u003c\/h2\u003e\n\u003cp\u003edNTP Mix is a solution containing each of the four deoxynucleotides as follows:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e10 mM dATP\u003c\/li\u003e\n\u003cli\u003e10 mM dCTP\u003c\/li\u003e\n\u003cli\u003e10 mM dGTP\u003c\/li\u003e\n\u003cli\u003e10 mM dTTP\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003edNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing standard PCR components. A single band of approximately 500 base pairs was visualized following electrophoresis, confirming high performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Quality Tests --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease-Exonuclease\u003c\/strong\u003e\u003cbr\u003eOne μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM TrisHCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease (Nickase)\u003c\/strong\u003e\u003cbr\u003eOne μg of pBR322 DNA was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circularDNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRNase\u003c\/strong\u003e\u003cbr\u003eTwo μg of transfer RNA were incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Documents --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Info.DD0056.pdf?v=1734165227\" target=\"_blank\"\u003e Download Info \u003c\/a\u003e \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.DD0056_9K-003-0006-DNTP_10mM.pdf?v=1734165227\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/technicalsheet.dntp_V2_23.pdf?v=1734165270\" target=\"_blank\"\u003e Download Technical Bulletin \u003c\/a\u003e \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Disclaimer --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDisclaimer\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin: 0; color: #d93025; font-weight: bold;\"\u003eFor laboratory research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eddntp\u003c\/div\u003e","brand":"BioBasic","offers":[{"title":"0.5mL","offer_id":44910007419061,"sku":"DD0056-0.5mL","price":20.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dntp-am-e-v1.png?v=1734511986"},{"product_id":"dntp-mixture-25mm-biofargo","title":"dNTP mixture (25mM)","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eOur dNTPs are analytically tested to ensure a purity of \u0026gt;99% by HPLC, guaranteeing optimal performance in demanding PCR applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eParameter\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e ≥ 99% (HPLC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNumber of Containers\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eFreezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e12352208\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNucleic Acid Bases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eDG\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003e(-15 to -20)℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eStock\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid #e0e0e0; text-align: center;\"\u003eUsually in Stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Summary --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSummary\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border: 1px solid #eee;\"\u003e\n\u003cp\u003eDNase, RNase: None detected.\u003c\/p\u003e\n\u003cp\u003eSuitable for use in the Polymerase Chain Reaction (PCR).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- PCR Suitability --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePCR Suitability\u003c\/h2\u003e\n\u003cp\u003edNTP Mix is a solution containing each of the four deoxynucleotides as follows:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e25 mM dATP \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e25 mM dCTP\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e25 mM dGTP \u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e25 mM dTTP\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003edNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing10 mM Tris-HCl, pH 8.3 at 25 °C, 50 mM KCl, 1.5 mM MgCl2, 0.001% (w\/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng\/100 μl, and Taq DNA polymerase at 2.5 units\/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments, and 72 °C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Quality Tests --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease-Exonuclease\u003c\/strong\u003e\u003cbr\u003eOne μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM TrisHCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease (Nickase)\u003c\/strong\u003e\u003cbr\u003eOne μg of pBR322 DNA was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circular DNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRNase\u003c\/strong\u003e\u003cbr\u003eTwo μg of transfer RNA were incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Documents --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Info.DD0057-v2.pdf?v=1734165557\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Info \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.DD0057-dNTP_mixture_25mM_6ba51bf2-21f8-4537-a14e-f007ebff1feb.pdf?v=1776072655\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/technicalsheet.dNTP_V1_23.pdf?v=1734165607\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download Technical Bulletin \u003c\/a\u003e \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Disclaimer --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDisclaimer\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin: 0; color: #d93025; font-weight: bold;\"\u003eFor laboratory research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eddntp\u003c\/div\u003e","brand":"BioBasic","offers":[{"title":"0.5mL","offer_id":44910007779509,"sku":"DD0057-0.5mL","price":47.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_5179-dntp-edit-whitebackground-compressed-v3.png?v=1734511983"},{"product_id":"dntp-set-100mm-each-biofargo","title":"dNTP Set (100mM each)","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eOur dNTPs are analytically tested to ensure a purity of \u0026gt;99% by HPLC, guaranteeing optimal performance in demanding PCR applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 99.9574%; border-collapse: collapse; border: 1px solid rgb(224, 224, 224);\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid rgb(224, 224, 224); width: 57.9016%;\"\u003eParameter\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid rgb(224, 224, 224); width: 42.0764%;\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e ≥ 99% (HPLC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e0.4 mL; 2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e0.1 mL; 0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eNumber of Containers\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eFreezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e12352208\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eNucleic Acid Bases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eDG\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003e(-15 to -20)℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 57.9016%;\"\u003eStock\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border: 1px solid rgb(224, 224, 224); text-align: center; width: 42.0764%;\"\u003eUsually in Stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Summary --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSummary\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border: 1px solid #eee;\"\u003e\n\u003cp\u003eDNase, RNase: None detected.\u003c\/p\u003e\n\u003cp\u003eSuitable for use in the Polymerase Chain Reaction (PCR).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- PCR Suitability --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePCR Suitability\u003c\/h2\u003e\n\u003cp\u003edNTP set is a solution containing dATP, dCTP, dGTP and dTTP each 100mM. pH 7.0:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e100 mM dATP (pH 7.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e100 mM dCTP (pH 7.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e100 mM dGTP  (pH 7.0)\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e100 mM dTTP (pH 7.0)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003edNTP Set Solution was tested at a final concentration of 200 μM in a reaction mixture containing10 mM Tris-HCl, pH 8.3 at 25 °C, 50 mM KCl, 1.5 mM MgCl2, 0.001% (w\/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng\/100 μl, and Taq DNA polymerase at 2.5 units\/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments, and 72 °C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Quality Tests --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease-Exonuclease\u003c\/strong\u003e\u003cbr\u003eOne μg of λ Hind III fragments was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM TrisHCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable. \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEndonuclease (Nickase)\u003c\/strong\u003e\u003cbr\u003eOne μg of pBR322 DNA was incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circularDNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRNase\u003c\/strong\u003e\u003cbr\u003eTwo μg of transfer RNA were incubated for 16 hours at 37 °C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- Documents --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Info.DD0058_3056a672-408f-4e3b-a7a5-ea53bbec9467.pdf?v=1776156149\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Data Sheet \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.DD0058-DNTP_100mM.pdf?v=1734165951\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/technicalsheet.dntp_V2_23_1.pdf?v=1734165951\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download Technical Bulletin \u003c\/a\u003e \u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Disclaimer --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDisclaimer\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 15px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin: 0; color: #d93025; font-weight: bold;\"\u003eFor laboratory research use only.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eddntp\u003c\/div\u003e","brand":"BioBasic","offers":[{"title":"4*0.1mL","offer_id":44910008074421,"sku":"DD0058-0.4mL","price":58.0,"currency_code":"USD","in_stock":true},{"title":"4*0.5mL","offer_id":44910008107189,"sku":"DD0058-2mL","price":244.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_0364_v3_sm.png?v=1734511982"},{"product_id":"pcr-hero-™","title":"PCR Hero ™","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eThis product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003cp\u003eTaq Hero is a new generation of DNA polymerase modified by Foregene based on Taq enzyme.\u003c\/p\u003e\n\u003ch2\u003eSpecifications:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigher fidelity: 6 times that of ordinary Taq enzyme;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFaster amplification speed: the amplification speed is 5-10sec\/kb, which is 6-12 times that of ordinary Taq enzyme;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMore template adaptability: it can efficiently amplify various complex DNA templates with high GC content and difficult to amplify;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigher Amplification efficiency: the number of amplification cycles is lower than that of ordinary Taq enzyme;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStronger Environmental tolerance: placed at 37°C for a week, maintaining more than 90% activity;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct components\u003c\/h2\u003e\n\u003cp\u003e2× PCR HeroTM Mix include:0.1U Taq Hero\/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8),100mM KCl, 5mM MgCl2,other stabilizers and enhancers.\u003c\/p\u003e\n\u003ch2\u003eKit Series\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01013:2× PCR HeroTM Mix 1.7ml ×3\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01014:2× PCR HeroTM Mix 1.7ml ×6\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01015:2× PCR HeroTM Mix 1.7ml ×24\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cp\u003eThe 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePCR amplification of DNA fragments\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA labeling\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA sequencing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePCR plus A tail\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePractical examples\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.Reaction System\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e2× PCR HeroTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTemplate DNA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003exμl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 1(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 2(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eFill to 20μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTotal volume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e20µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.Reaction conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e3min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e10sec\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e25-40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e55-65°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e10sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle; height: 18px;\"\u003e≥10sec (5-20sec\/kb)\u003csup\u003e1*\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1*: If using plasmid as template, the extension speed is 5sec\/kb, we recommend using 10sec\/kb; if using genomic DNA as the template, the extension speed is 10sec\/kb, we recommend using 20sec\/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec\/kb on the original basis.\u003c\/p\u003e\n\u003ch2\u003eStorage and Shelf life\u003c\/h2\u003e\n\u003cp\u003eIt can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PCR_Hero_Instruction_Manual-V1.0.docx?v=1752830384\"\u003e\u003cstrong\u003ePCR Hero™ Instruction Manual-V1.0\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003epcr buffer,master mix\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"5ml","offer_id":51366216794293,"sku":"PH-01013","price":122.0,"currency_code":"USD","in_stock":true},{"title":"40ml","offer_id":51366216827061,"sku":"PH-01015","price":779.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PCRHero.jpg?v=1752829796"},{"product_id":"pcr-hero-™-with-dye","title":"PCR Hero ™（With Dye)","description":"\u003ch2\u003e Descprition\u003c\/h2\u003e\n\u003cp\u003eThis product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003cp\u003eTaq Hero is a new generation of DNA polymerase modified by Foregene based on Taq enzyme.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01013-B:2× PCR HeroTM Mix (dye) 1.7ml ×3\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01014-B:2× PCR HeroTM Mix (dye) 1.7ml ×6\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePH-01015-B:2× PCR HeroTM Mix (dye) 1.7ml ×24\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cp\u003e2× PCR HeroTM Mix include:0.1U Taq Hero\/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8), 100mM KCl, 5mM MgCl2,other stabilizers and enhancers,DNA Loading Buffer.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cp\u003eThe 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePCR amplification of DNA fragments\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA labeling\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA sequencing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePCR plus A tail\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePractical examples\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.Reaction System\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e2× PCR HeroTM Mix(dye)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTemplate DNA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003exμl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 1(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 2(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eFill to 20μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTotal volume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e20µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.Reaction conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e3min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e25-40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e55-65°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e≥10sec (5-20sec\/kb)1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1*: If using plasmid as template, the extension speed is 5sec\/kb, we recommend using 10sec\/kb; if using genomic DNA as the template, the extension speed is 10sec\/kb, we recommend using 20sec\/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec\/kb on the original basis.\u003c\/p\u003e\n\u003ch2\u003eStorage and Shelf life\u003c\/h2\u003e\n\u003cp\u003eIt can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PCR_Hero_With_Dye_Instruction_Manual-V1.0.docx?v=1752831098\"\u003e\u003cstrong\u003ePCR Hero™（With Dye) Instruction Manual-V1.0\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003epcr buffer,master mix\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"5ml","offer_id":51366249365685,"sku":"PH-01013-B","price":122.0,"currency_code":"USD","in_stock":true},{"title":"40ml","offer_id":51366249398453,"sku":"PH-01015-B","price":779.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PH-01013-BPCRHero_WithDye_4.jpg?v=1752831079"},{"product_id":"2-high-efficiency-pcr-reaction-premix-system-pcr-easytm","title":"2×High-efficiency PCR reaction premix system PCR EasyTM","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eThis product contains our company's unique Foregene Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The unique reaction system and high-efficiency Taq DNA Polymerase make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003cp\u003eDuring PCR amplification, specific amplified bands can be obtained in 5-10 cycles less than similar products at home and abroad, and the target gene can be detected and cloned from trace and trace samples at a lower number of cycles. The optimized reaction system can greatly reduce human error; it is suitable for the amplification and detection of conventional PCR reactions, complex templates, and trace templates.\u003c\/p\u003e\n\u003ch2\u003eProduct components\u003c\/h2\u003e\n\u003cp\u003e2× PCR EasyTM Mix include:0.1U Foregene Taq DNA Polymerase\/μl, 400 μM dNTPs, 20 mM Tris-HCl(pH8.8), 100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cp\u003eThe 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.\u003c\/p\u003e\n\u003ch2\u003eKit Application\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePCR amplification of DNA fragments\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA labeling\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA sequencing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePCR plus A tail\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstructions\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003ePCR EasyTM is convenient and quick to use, and can avoid contamination during PCR operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, just take half the volume of the reaction system (for example, if the reaction system is 50μl, take 25μl 2× PCR EasyTM Mix solution) 2× PCR EasyTM Mix, add template and primers, and add deionized water to make up the volume to make the reaction system. The reaction can be carried out when the concentration of PCR Mix is 1×.\u003c\/p\u003e\n\u003ch2\u003ePractical examples\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.Reaction System\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e2× PCR EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTemplate DNA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003exμl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 1(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePrimer 2(10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.4μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eFill to 20μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTotal volume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e20µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.Reaction conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e3min\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003cbr\u003e25-40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e55°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e20sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2kb\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.\u003c\/p\u003e\n\u003ch2\u003eStorage and Shelf life\u003c\/h2\u003e\n\u003cp\u003eIt can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003epcr buffer,master mix\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"1ml","offer_id":51366687146165,"sku":"DP-20031","price":57.0,"currency_code":"USD","in_stock":true},{"title":"3×1.7ml","offer_id":51366687178933,"sku":"DP-20032","price":245.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DP-200322xHigh-efficiencyPCRreactionpremixsystem_4.jpg?v=1752835949"},{"product_id":"aptamer-screening-experimental-kit","title":"Aptamer Screening Experimental Kit","description":"\u003cdiv class=\"bf-product-container\" style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1100px; margin: 0 auto; padding: 15px; box-sizing: border-box;\"\u003e\n    \n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n        \u003ch2 style=\"color: #0056b3; font-size: 24px; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-bottom: 20px;\"\u003eDescription\u003c\/h2\u003e\n        \u003cdiv style=\"background-color: #f8f9fa;  padding: 20px; border-radius: 4px; box-shadow: 0 2px 4px rgba(0,0,0,0.05);\"\u003e\n            \u003cp style=\"margin-top: 0; font-size: 15px;\"\u003eThe Aptamer Screening Experimental Kit is an integrated laboratory reagent and consumable system designed to support nucleic acid-based affinity screening experiments. The kit consolidates all essential reagents, buffers, amplification mixes, electrophoresis materials, and supporting tools required to perform iterative nucleic acid binding, amplification, separation, and recovery workflows in a single package.\u003c\/p\u003e\n            \u003cp style=\"margin-bottom: 0; font-size: 15px;\"\u003eAll components are provided in ready-to-use formats and are optimized for compatibility with magnetic bead handling, PCR and qPCR amplification, denaturing PAGE separation, and nucleic acid purification procedures. This kit is intended to simplify experimental setup, reduce sourcing complexity, and improve workflow consistency for laboratories conducting nucleic acid screening and enrichment experiments.\u003c\/p\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cstyle\u003e\n        .bf-grid { display: grid; grid-template-columns: repeat(2, 1fr); gap: 25px; margin-bottom: 35px; }\n        .bf-card { background: #fff; border: 1px solid #e1e4e8; border-radius: 8px; padding: 20px; box-shadow: 0 3px 10px rgba(0,0,0,0.05); }\n        .bf-card h3 { color: #0056b3; font-size: 20px; margin-top: 0; margin-bottom: 15px; border-bottom: 1px solid #eee; padding-bottom: 10px; }\n        .bf-list { list-style: none; padding: 0; margin: 0; }\n        .bf-list-item { margin-bottom: 12px; font-size: 14px; position: relative; padding-left: 20px; }\n        .bf-list-item::before { content: \"•\"; color: #0056b3; font-weight: bold; position: absolute; left: 0; }\n        \n        \/* Mobile Optimization *\/\n        @media (max-width: 768px) {\n            .bf-grid { grid-template-columns: 1fr; }\n            .bf-table-wrapper { overflow-x: auto; -webkit-overflow-scrolling: touch; border: 1px solid #ddd; border-radius: 8px; position: relative; }\n            .bf-table-wrapper::after { content: \"← Scroll to view more →\"; display: block; text-align: center; font-size: 12px; color: #999; padding: 5px; background: #f9f9f9; }\n        }\n    \u003c\/style\u003e\n\n    \u003cdiv class=\"bf-grid\"\u003e\n        \u003cdiv class=\"bf-card\"\u003e\n            \u003ch3\u003eFeatures\u003c\/h3\u003e\n            \u003cul class=\"bf-list\"\u003e\n                \u003cli class=\"bf-list-item\"\u003e\n\u003cstrong\u003eComprehensive reagent coverage:\u003c\/strong\u003e All key reagents, buffers, and consumables required for nucleic acid screening workflows are included.\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003e\n\u003cstrong\u003eWorkflow consistency:\u003c\/strong\u003e Reduces variability caused by sourcing reagents from multiple suppliers.\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003e\n\u003cstrong\u003eReady-to-use system:\u003c\/strong\u003e Components are supplied in formats suitable for direct laboratory use.\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003e\n\u003cstrong\u003eCompatibility with standard equipment:\u003c\/strong\u003e Designed to work with commonly available magnetic racks, thermocyclers, electrophoresis systems, and imaging devices.\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003e\n\u003cstrong\u003eModular experimental support:\u003c\/strong\u003e Individual components can be used independently or as part of an integrated workflow.\u003c\/li\u003e\n            \u003c\/ul\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv class=\"bf-card\"\u003e\n            \u003ch3\u003eApplications\u003c\/h3\u003e\n            \u003cp style=\"font-size: 14px; font-weight: 600; margin-bottom: 10px;\"\u003eSuitable for research laboratories performing:\u003c\/p\u003e\n            \u003cul class=\"bf-list\" style=\"color: #555;\"\u003e\n                \u003cli class=\"bf-list-item\"\u003eNucleic acid binding and enrichment experiments\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003eAffinity screening and molecular interaction studies\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003eOptimization of nucleic acid amplification workflows\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003ePAGE-based nucleic acid separation and analysis\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003eExperimental method development and validation\u003c\/li\u003e\n                \u003cli class=\"bf-list-item\"\u003eTraining, teaching, and protocol standardization\u003c\/li\u003e\n            \u003c\/ul\u003e\n            \u003cp style=\"font-size: 12px; color: #888; font-style: italic; margin-top: 15px; border-top: 1px dotted #ccc; padding-top: 10px;\"\u003eApplications may vary depending on user-defined experimental design and target systems.\u003c\/p\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n        \u003ch2 style=\"color: #0056b3; font-size: 24px; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-bottom: 20px;\"\u003eComponents\u003c\/h2\u003e\n        \u003cdiv class=\"bf-table-wrapper\"\u003e\n            \u003ctable style=\"width: 100%; border-collapse: collapse; min-width: 600px; background: #fff;\"\u003e\n                \u003cthead\u003e\n                    \u003ctr style=\"background-color: #0056b3; color: #ffffff;\"\u003e\n                        \u003cth style=\"padding: 12px 15px; text-align: left; border: 1px solid #004a99;\"\u003eComponent\u003c\/th\u003e\n                        \u003cth style=\"padding: 12px 15px; text-align: center; border: 1px solid #004a99; width: 180px;\"\u003eCat. No.\u003c\/th\u003e\n                    \u003c\/tr\u003e\n                \u003c\/thead\u003e\n                \u003ctbody style=\"font-size: 14px;\"\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eCarboxyl Magnetic Beads\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-1\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003essDNA Library (11 bp Fixed Region + 80 nt Random Region)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-2\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eSodium Acetate Buffer (pH 4.0)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-3-1\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eSodium Acetate Buffer (pH 4.5)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-3-2\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eSodium Acetate Buffer (pH 5.0)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-3-3\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eEDC\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-4\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eNHS\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-5\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eEthanolamine, 1 M (pH 8.5)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-6\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eBSA (1 mg\/mL)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSEP-201903-7\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003ePCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eESE-201801-4\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eqPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eESE-201801-5\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003e0.5 mL Centrifuge Tubes\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eESE-201801-6\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eEmulsion PCR Droplet Oil\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eEPO-100\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eDenaturing PAGE Running Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eDDP-100-1\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003e10% APS (Ammonium Persulfate)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eDDP-100-2\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eTEMED\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eDDP-100-3\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003e2× TBE Urea Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eTLB-5\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eFluorescent ssDNA Ladder\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eSDL-100\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eMicro Nucleic Acid Dialysis Membrane\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eMDM-30-1\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eMicro Nucleic Acid Dialysis Device\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; font-family: monospace;\"\u003eMDM-40-2\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003ePreparative Electrophoresis Comb\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eSingle-edge Razor Blade\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eCotton Thread\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eUV Gel Cutting Flashlight\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eFlat-tip Carbon Fiber Tweezers\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eStrong Magnet\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eSmall Scissors\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003eWater Bath Float\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr style=\"background-color: #fcfcfc;\"\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee;\"\u003e1.5 mL Tube Safety Clip\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 15px; border: 1px solid #eee; text-align: center; color: #ccc;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n                \u003c\/tbody\u003e\n            \u003c\/table\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"margin-bottom: 35px; background: linear-gradient(135deg, #f0f7ff 0%, #ffffff 100%); border-radius: 8px; padding: 25px; border: 1px solid #d0e3f7;\"\u003e\n        \u003ch2 style=\"color: #0056b3; font-size: 22px; margin-top: 0; margin-bottom: 15px;\"\u003eExperimental Workflow Compatibility\u003c\/h2\u003e\n        \u003cp style=\"font-size: 14px; margin-bottom: 15px; color: #444;\"\u003eSupports the following experimental operations:\u003c\/p\u003e\n        \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fill, minmax(280px, 1fr)); gap: 10px;\"\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Magnetic bead immobilization\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Chemical coupling reactions\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e PCR and qPCR amplification\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Emulsion PCR workflows\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Denaturing PAGE\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Nucleic acid size analysis\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Gel extraction \u0026amp; recovery\u003c\/div\u003e\n            \u003cdiv style=\"font-size: 13.5px; color: #444; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"color: #28a745; margin-right: 8px;\"\u003e✓\u003c\/span\u003e Dialysis purification\u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"background-color: #fffbe6; border: 1px solid #ffe58f; border-radius: 6px; padding: 15px;\"\u003e\n        \u003ch3 style=\"color: #856404; font-size: 16px; margin-top: 0; margin-bottom: 8px;\"\u003eNotes:\u003c\/h3\u003e\n        \u003cul style=\"margin: 0; padding-left: 20px; color: #856404; font-size: 13px;\"\u003e\n            \u003cli\u003eThis product is supplied for \u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e.\u003c\/li\u003e\n            \u003cli\u003eExperimental outcomes depend on user-defined protocols and conditions.\u003c\/li\u003e\n            \u003cli\u003eThis kit does not include targets or experimental design services.\u003c\/li\u003e\n            \u003cli\u003eFollow standard laboratory safety and handling practices.\u003c\/li\u003e\n        \u003c\/ul\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Aptamy","offers":[{"title":"Default Title","offer_id":52286259364021,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_c5daaaa4-8791-4336-b33f-89fa5971125f.jpg?v=1772086881"}],"url":"https:\/\/biofargo.com\/collections\/standard-pcr-reagents.oembed","provider":"Biofargo","version":"1.0","type":"link"}