{"title":"Speedycut","description":"","products":[{"product_id":"supercut™-xhoⅰ-biosci","title":"XhoI-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…C↓T C G A G…3'\u003cbr\u003e3'…G A G C T↑C…5'\u003cbr\u003eIsoschizomers*: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231206151502_160x160.png?v=1701846927\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ XhoⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™XhoⅠ.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ AvrII was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ XhoⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria- Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be\u003cbr\u003eexpressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eTo 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003eTo 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eTo 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently suck or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃ or add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eimpaired\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"5000 units","offer_id":43541335736501,"sku":"8052441","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052441SuperCut_XhoIFastRestrictionEndonucleasespng.png?v=1732886496"},{"product_id":"supercut™-ndeⅰ-biosci","title":"NdeⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...C A↓T A T G...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...G T A T ↑A C...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: FauNDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206161534_160x160.png?v=1701850606\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NdeⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ NdeⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ NdeⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NdeⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NdeⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NdeⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NdeⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™  NdeⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"2000 units","offer_id":43541352087733,"sku":"8052251","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_91317f02-dc20-4d8e-823b-55878e93d954.jpg?v=1753176201"},{"product_id":"supercut™-nsiⅰ","title":"NsiⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...A T G C A↓T...3'\u003cbr\u003e3'...T↑A C G T A...5'\u003cbr\u003eIsoschizomers*: EcoT22I, Mph1103I, Zsp2IDI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206165333_160x160.png?v=1701852836\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ NsiⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 1 μL of SuperCut™ NsiⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NsiⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NsiⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NsiⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NsiⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e14\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e9\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500 units","offer_id":43541364605109,"sku":"8052291","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052291SuperCut_NsiIpng.png?v=1732878695"},{"product_id":"supercut™-hindⅲ-biosci","title":"HindⅢ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…A↓A G C T T…3'\u003cbr\u003e3'…T T C G A↑A…5'\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206170842_160x160.png?v=1701853741\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ HindⅢ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ HindⅢ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ HindⅢ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ HindⅢ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ HindⅢ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ HindⅢ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e12\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eSome impaired\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"10000 units","offer_id":43541383184565,"sku":"8052181","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052181SuperCut_Hind_FastRestrictionEndonucleasespng.png?v=1732874002"},{"product_id":"speedycut-dpni-kleanab","title":"SpeedyCut DpnI-KleanAB","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eSpecification\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 234px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFull name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSpeedyCut DpnI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFast enzymatic digestion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSummary\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFeatures\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComposition\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecognition Site\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5'…-GA m6↓TC-…3' 3'…-C T↑A m6G-…5'\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e10X SpeedyOne Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecommended Reaction Temperature\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eMethylation Effects\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eDam: Cannot cleave dam– DNA; Dcm: Methylation does not affect;CpG: Cleavage is blocked.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eThermal Inactivation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFast enzymatic digestion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e-20°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Sangon","offers":[{"title":"50 RXNS","offer_id":43838990319797,"sku":"P310013-0050","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310013_1.jpg?v=1732883306"},{"product_id":"bsmbi","title":"BsmBI","description":"\u003ch2 class=\"MsoNormal\"\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1000 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBsmBI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eIt is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBsmBI is the isoschizoid enzyme of the SpeedyCut series restriction enzyme Esp3I, but because the reaction buffer is optimized to maximize the activity of BsmBI endonuclease, it becomes an independent system Buffer, which cannot be shared with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eStability is better than that of isoschizozyme Esp3I for a large number of linearized plasmid preparations.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1ul BsmBI,1X BsmBI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CGTCTC(N)1↓…-3' \u003cbr\u003e3'…-GCAGAG(N)5↑…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1X BsmBI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e55°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOne unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 5 μl reaction system at 5℃ for 1 hour.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences overlap completely, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage may be blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 8°C for 2 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310001_manual_624855c5-e0b8-467f-99a2-ffc3a90fef40.pdf?v=1753412582\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310001_MSDS.pdf?v=1753412513\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1000U","offer_id":52400957751477,"sku":"P310001-1000U","price":141.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_bsmbl.jpg?v=1751472363"},{"product_id":"bspqi","title":"BspQI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eIt is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI is the isoschizome of the SpeedyCut series restriction enzyme SapI, but because the reaction buffer is consistent with the BsmBI enzyme reaction system, the BspQI endonuclease can exert maximum activity, so it can be shared with the B600199 reaction buffer, but not with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI is a type of Type IIs endonuclease that recognizes non-palindromic sequences and cuts them outside of the recognition sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50ul BspQI, 10X BspQI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GCTCTTC(N)1↓…-3' 3'…-CGAGAAG(N)4↑…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X BspQI Buffer (Dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOne unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 50 μl reaction system at 50℃ for 1 hour.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50ul BspQI, 10X BspQI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003e\n\u003cspan\u003e\u003c\/span\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310002_manual.pdf?v=1753412653\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310002_MSDS.pdf?v=1753412727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500U","offer_id":52400957817013,"sku":"P310002-500U","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_bspql.png?v=1751472361"},{"product_id":"speedycut-sfii","title":"SpeedyCut SfiI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SfiI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GGCCNNNN↓NGGCC-…3' 3'…-CCGGN↑NNNNCCGG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 50°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Completely overlapping sequences, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310003_manual.pdf?v=1753413208\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310003_MSDS.pdf?v=1753413162\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400957882549,"sku":null,"price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-pspg-i","title":"SpeedyCut PspG I","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PspG I\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-↓CCWGG-…3' 3'…-GGWCC↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e75°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 75°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA（Dcm-）in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_EN_P.pdf?v=1752658123\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_manual.pdf?v=1753413684\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_MSDS.pdf?v=1753413708\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400957948085,"sku":"P310004-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-agei","title":"SpeedyCut AgeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AgeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CCGGT-…3' 3'…-TGGCC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences overlap completely, cleavage blocked; EcoKI:Methylation does not affect; EcoBI:Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310005_manual.pdf?v=1753413996\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310005_MSDS.pdf?v=1753413970\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400958013621,"sku":"P310005-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-apalⅰ","title":"SpeedyCut ApaLⅠ","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut ApaLⅠ\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5′-G↓TGCAC-3′ 3'-CACGT↑G-5′\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310006_manual.pdf?v=1753414295\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310006_MSDS.pdf?v=1753414315\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"150 RXNS","offer_id":44611372908725,"sku":"P310006-150 RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-asci","title":"SpeedyCut AscI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AscI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-GG↓CGCGCC-3' 3'-CCGCGC↑GG-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_manual.pdf?v=1753415117\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_MSDS.pdf?v=1753345000\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_MSDS.pdf?v=1753415101\" type=\"application\/pdf\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982327477,"sku":"P310007-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bamhi","title":"SpeedyCut BamHI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BamHI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-G↓GATCC-3' 3'-CCTAG↑G-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOnly a small fraction can be inactivated by heating at 80°C for 20 minutes, and most of them cannot be thermoinactivated, so use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400982294709,"sku":"P310008-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bcli","title":"SpeedyCut BclI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BclI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-T↓GATCA-3' 3'-ACTAG↑T-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310009_manual.pdf?v=1753415983\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310009_MSDS.pdf?v=1753416010\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"125RXNS","offer_id":52400982261941,"sku":"P310009-125RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bglii","title":"SpeedyCut BglII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BglII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓GATCT-…3' 3'…-TCTAG↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310010_manual.pdf?v=1753768852\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310010_MSDS.pdf?v=1753768409\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400982229173,"sku":"P310010-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bsteii","title":"SpeedyCut BstEII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BstEII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓GTNACC-…3' 3'…-CCANTG↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect;CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocumnets\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100011_manual.pdf?v=1753769169\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100011_MSDS.pdf?v=1753769120\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400982196405,"sku":"P310011-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-clai","title":"SpeedyCut ClaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut ClaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AT↓CGAT-…3' 3'…-TAGC↑TA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100012_manual.pdf?v=1753769280\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310012_MSDS.pdf?v=1753769319\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982163637,"sku":"P310012-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-dpnii","title":"SpeedyCut DpnII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut DpnII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-↓GATC-…3' 3'…- CTAG↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocumnets\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310014_manual.pdf?v=1753772780\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310014_MSDS.pdf?v=1753772733\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982130869,"sku":"P310014-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-eagi","title":"SpeedyCut EagI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EagI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓GGCCG-…3' 3'…-GCCGG↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310015_manual.pdf?v=1753773225\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310015_MSDS.pdf?v=1753773260\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400982098101,"sku":"P310015-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ecori","title":"SpeedyCut EcoRI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EcoRI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-G↓AATTC-3' 3'-CTTAA↑G-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"600RXNS","offer_id":52400982065333,"sku":"P310016-600RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ecorv","title":"SpeedyCut EcoRV","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EcoRV\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GAT↓ATC-…3' 3'…-CTA↑TAG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310017_manual.pdf?v=1753773392\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310017_MSDS.pdf?v=1753773422\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981999797,"sku":"P310017-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-fspi","title":"SpeedyCut FspI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut FspI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TGC↓GCA-…3' 3'…-ACG↑CGT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked when sequences overlap; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310018_manual.pdf?v=1753773505\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310018_MSDS.pdf?v=1753773530\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981934261,"sku":"P310018-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hindiii","title":"SpeedyCut HindIII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HindIII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓AGCTT-…3' 3'…-TTCGA↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310019_MSDS.pdf?v=1753774476\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981901493,"sku":"P310019-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hinfi","title":"SpeedyCut HinfI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HinfI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓ANTC-…3' 3'…-CTNA↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310020_manual.pdf?v=1753773935\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310020_MSDS.pdf?v=1753773927\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981868725,"sku":"P310020-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hpai","title":"SpeedyCut HpaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HpaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GTT↓AAC-…3' 3'…-CAA↑TTG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310021_manual.pdf?v=1753773997\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310021_MSDS.pdf?v=1753774015\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981835957,"sku":"P310021-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-kpni","title":"SpeedyCut KpnI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut KpnI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GGTAC↓C-…3' 3'…-C↑CATGG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310022_manual.pdf?v=1753774147\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310022_MSDS.pdf?v=1753774132\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981803189,"sku":"P310022-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-mlui","title":"SpeedyCut MluI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut MluI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CGCGT-…3' 3'…-TGCGC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310023_manual.pdf?v=1753774246\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310023_MSDS.pdf?v=1753774246\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981770421,"sku":"P310023-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-mnli","title":"SpeedyCut MnlI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut MnlI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCTC(N)7↓-…3' 3'…-GGAG(N)6↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310024_manual.pdf?v=1753774563\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310024_MSDS.pdf?v=1753774577\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981737653,"sku":"P310024-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ncoi","title":"SpeedyCut NcoI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NcoI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CATGG-…3' 3'…-GGTAC↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310025_manual.pdf?v=1753774687\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310025_MSDS.pdf?v=1753774669\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"30RXNS","offer_id":52400981704885,"sku":"P310025-30RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ndei","title":"SpeedyCut NdeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NdeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CATGG-…3' 3'…-GGTAC↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310026_manual.pdf?v=1753774909\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310026_MSDS.pdf?v=1753774945\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981672117,"sku":"P310026-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nhei","title":"SpeedyCut NheI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NheI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓CTAGC-…3' 3'…-CGATC↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310027_manual.pdf?v=1753775119\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310027_MSDS.pdf?v=1753775106\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"30RXNS","offer_id":52400981639349,"sku":"P310027-30RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-noti","title":"SpeedyCut NotI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NotI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GC↓GGCCGC-…3' 3'…-CGCCGG↑CG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg N85 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981606581,"sku":"P310028-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nspv","title":"SpeedyCut NspV","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NspV\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TT↓CGAA-…3' 3'…-AA↑GCTT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310029_manual.pdf?v=1753775953\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310029_MSDS.pdf?v=1753775954\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981573813,"sku":"P310029-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nrui","title":"SpeedyCut NruI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NruI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TCG↓CGA-…3' 3'…-AGC↑GCT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Sequences may overlap and cleavage blocked; Dcm: Methylation does not affect; CpG: Cleavage is blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310030_manual.pdf?v=1753776075\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310030_MSDS.pdf?v=1753776060\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981541045,"sku":"P310030-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-pvuii","title":"SpeedyCut PvuII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PvuII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CAG↓CTG-…3' 3'…-GTC↑GAC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310031_manual.pdf?v=1753776163\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310031_MSDS.pdf?v=1753776176\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981508277,"sku":"P310031-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-paci","title":"SpeedyCut PacI","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e \u003cmeta name=\"viewport\" content=\"width=device-width, initial-scale=1.0\"\u003e\u003cmeta name=\"description\" content=\"SpeedyCut PacI restriction enzyme for rapid digestion of plasmid DNA, PCR products and genomic DNA using universal SpeedyOne Buffer at 37°C.\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n.speedycut-product-page,\n.speedycut-product-page * {\n  box-sizing: border-box;\n}\n\n.speedycut-product-page {\n  font-family: Arial, Helvetica, sans-serif;\n  font-size: 16px;\n  line-height: 1.65;\n  color: #222222;\n}\n\n.speedycut-product-page section {\n  margin-bottom: 36px;\n}\n\n.speedycut-product-page h2 {\n  margin: 0 0 16px;\n  font-size: 24px;\n  line-height: 1.3;\n  font-weight: 600;\n}\n\n.speedycut-product-page h3 {\n  margin: 24px 0 10px;\n  font-size: 18px;\n  line-height: 1.4;\n  font-weight: 600;\n}\n\n.speedycut-product-page p {\n  margin: 0 0 14px;\n}\n\n.speedycut-product-page ul,\n.speedycut-product-page ol {\n  margin: 0 0 16px;\n  padding-left: 24px;\n}\n\n.speedycut-product-page li {\n  margin-bottom: 8px;\n}\n\n.speedycut-table-wrapper {\n  width: 100%;\n  overflow-x: auto;\n  margin: 16px 0;\n  -webkit-overflow-scrolling: touch;\n}\n\n.speedycut-product-page table {\n  width: 100%;\n  min-width: 620px;\n  border-collapse: collapse;\n}\n\n.speedycut-product-page th,\n.speedycut-product-page td {\n  border: 1px solid #cccccc;\n  padding: 11px 12px;\n  text-align: left;\n  vertical-align: top;\n}\n\n.speedycut-product-page th {\n  font-weight: 600;\n}\n\n.speedycut-product-page pre {\n  margin: 12px 0;\n  padding: 14px;\n  border: 1px solid #cccccc;\n  overflow-x: auto;\n  font-family: Consolas, Monaco, monospace;\n  font-size: 15px;\n  line-height: 1.6;\n  white-space: pre-wrap;\n}\n\n.speedycut-product-page details {\n  border-top: 1px solid #cccccc;\n  padding: 14px 0;\n}\n\n.speedycut-product-page details:last-child {\n  border-bottom: 1px solid #cccccc;\n}\n\n.speedycut-product-page summary {\n  cursor: pointer;\n  font-weight: 600;\n  line-height: 1.5;\n}\n\n.speedycut-product-page details p {\n  margin: 12px 0 0;\n}\n\n.speedycut-product-page a {\n  color: inherit;\n  text-decoration: underline;\n}\n\n.speedycut-note {\n  font-size: 14px;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"speedycut-product-page\"\u003e\n\u003c!-- ========================= DESCRIPTION ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRestriction digestion workflows can become time-consuming when different enzymes require separate reaction buffers, sequential digestion steps, or repeated buffer exchange. These additional steps can complicate plasmid DNA preparation, PCR product processing, genomic DNA digestion, and other molecular cloning workflows.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpeedyCut PacI\u003c\/strong\u003e is a fast PacI restriction enzyme designed to cleave DNA using the universal \u003cstrong\u003eSpeedyOne Buffer\u003c\/strong\u003e. The SpeedyCut restriction enzyme system supports rapid DNA digestion while allowing compatible enzymes from the series to be combined in a unified reaction system.\u003c\/p\u003e\n\u003cp\u003eSpeedyCut PacI recognizes the sequence \u003cstrong\u003e5′-TTAATTAA-3′\u003c\/strong\u003e and is suitable for restriction digestion of plasmid DNA, PCR products, and genomic DNA. Under the stated unit-definition conditions, 1 μL of enzyme completely digests 1 μg of pPacI DNA in a 20 μL reaction at 37°C within 15 minutes.\u003c\/p\u003e\n\u003cp\u003eThe enzyme can also be used for direct digestion of PCR reaction mixtures after amplification. Following digestion, SpeedyCut PacI may be heat-inactivated at 80°C for 20 minutes when enzyme inactivation is required.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= SPECIFICATIONS ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"speedycut-table-wrapper\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003eSpeedyCut PacI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eCatalog Number\u003c\/th\u003e\n\u003ctd\u003eP310032\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003ePacI Restriction Enzyme \/ Restriction Endonuclease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePackage\u003c\/th\u003e\n\u003ctd\u003e25 μL enzyme, 25 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eSupplied Component\u003c\/th\u003e\n\u003ctd\u003e25 μL SpeedyCut PacI enzyme and 10X SpeedyOne Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePrimary Application\u003c\/th\u003e\n\u003ctd\u003eFast restriction digestion of DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eRecognition Sequence\u003c\/th\u003e\n\u003ctd\u003e5′-TTAAT↓TAA-3′\u003cbr\u003e3′-AAT↑TAATT-5′\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eReaction Buffer\u003c\/th\u003e\n\u003ctd\u003e1X SpeedyOne Buffer prepared from the supplied 10X SpeedyOne Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eRecommended Reaction Temperature\u003c\/th\u003e\n\u003ctd\u003e37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eUnit Definition\u003c\/th\u003e\n\u003ctd\u003eAt 37°C in a 20 μL reaction system, 1 μL of enzyme completely digests 1 μg of pPacI DNA in 15 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePlasmid DNA Digestion Time\u003c\/th\u003e\n\u003ctd\u003e5–15 minutes at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePCR Product Digestion Time\u003c\/th\u003e\n\u003ctd\u003e15–30 minutes at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eGenomic DNA Digestion Time\u003c\/th\u003e\n\u003ctd\u003e30–60 minutes at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eThermal Inactivation\u003c\/th\u003e\n\u003ctd\u003e80°C for 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eDam Methylation Effect\u003c\/th\u003e\n\u003ctd\u003eNo effect; the recognition site does not overlap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eDcm Methylation Effect\u003c\/th\u003e\n\u003ctd\u003eNo effect; the recognition site does not overlap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eCpG Methylation Effect\u003c\/th\u003e\n\u003ctd\u003eNo effect; the recognition site does not overlap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eIntended Use\u003c\/th\u003e\n\u003ctd\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= FEATURES ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRapid PacI restriction digestion:\u003c\/strong\u003e supports complete digestion under the stated unit-definition conditions within 15 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUniversal SpeedyOne Buffer:\u003c\/strong\u003e SpeedyCut restriction enzymes use a common reaction buffer to simplify restriction enzyme workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMultiple DNA substrates:\u003c\/strong\u003e suitable for digestion of plasmid DNA, PCR products, and genomic DNA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDirect PCR product digestion:\u003c\/strong\u003e PCR reaction mixtures can be digested directly after amplification using the recommended protocol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDouble- and multiple-digestion support:\u003c\/strong\u003e compatible SpeedyCut enzymes can be combined in an appropriately adjusted reaction system.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHeat inactivation:\u003c\/strong\u003e enzyme activity can be stopped by incubation at 80°C for 20 minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuality-controlled enzyme performance:\u003c\/strong\u003e evaluated using long-incubation, ligation, recleavage, exonuclease, nonspecific endonuclease, and blue\/white cloning assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= APPLICATIONS ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003ch3\u003ePlasmid DNA Digestion\u003c\/h3\u003e\n\u003cp\u003eSpeedyCut PacI can be used for rapid restriction digestion of plasmid DNA. The recommended incubation time for plasmid substrates is 15 minutes at 37°C in 1X SpeedyOne Buffer.\u003c\/p\u003e\n\u003ch3\u003ePCR Product Digestion\u003c\/h3\u003e\n\u003cp\u003eThe enzyme is suitable for restriction digestion of purified PCR products. A reaction time of 15–30 minutes at 37°C is recommended for PCR product digestion.\u003c\/p\u003e\n\u003ch3\u003eDirect Digestion After PCR Amplification\u003c\/h3\u003e\n\u003cp\u003eSpeedyCut PacI may be added directly to a PCR reaction mixture after amplification. This workflow allows PCR product digestion without first preparing a separate purified DNA substrate, using the reaction composition described in the product manual.\u003c\/p\u003e\n\u003ch3\u003eGenomic DNA Digestion\u003c\/h3\u003e\n\u003cp\u003eSpeedyCut PacI can be used for restriction digestion of genomic DNA. Because genomic DNA may require a longer incubation period, the recommended digestion time is 30–60 minutes at 37°C.\u003c\/p\u003e\n\u003ch3\u003eRestriction Digestion in Molecular Cloning Workflows\u003c\/h3\u003e\n\u003cp\u003ePacI-cleaved DNA may be used in workflows that include DNA digestion, recovery, ligation, transformation, and subsequent analysis. Product quality control includes cleavage, ligation, recleavage, and blue\/white cloning assays.\u003c\/p\u003e\n\u003ch3\u003eDouble and Multiple Restriction Digestion\u003c\/h3\u003e\n\u003cp\u003eSpeedyCut PacI may be used with other compatible SpeedyCut restriction enzymes in a shared SpeedyOne Buffer reaction system. Reaction volume and enzyme volume should be adjusted according to the product instructions.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= RECOMMENDED DIGESTION PROTOCOL ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eRecommended DNA Digestion Protocol\u003c\/h2\u003e\n\u003cp\u003eThe following reaction is based on the 20 μL digestion system described in the product manual.\u003c\/p\u003e\n\u003cdiv class=\"speedycut-table-wrapper\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eVolume\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd\u003e16 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X SpeedyOne Buffer\u003c\/td\u003e\n\u003ctd\u003e2 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNA, 0.5–1 μg\/μL\u003c\/td\u003e\n\u003ctd\u003e1 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpeedyCut PacI\u003c\/td\u003e\n\u003ctd\u003e0.5–1 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eTotal Reaction Volume\u003c\/th\u003e\n\u003ctd\u003e20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003eAdd the reaction components in the amounts shown above.\u003c\/li\u003e\n\u003cli\u003eMix gently and briefly spin down the reaction tube.\u003c\/li\u003e\n\u003cli\u003eIncubate at 37°C for 15 minutes for plasmid DNA, 15–30 minutes for PCR products, or 30–60 minutes for genomic DNA.\u003c\/li\u003e\n\u003cli\u003eWhen required, incubate at 80°C for 20 minutes to heat-inactivate the enzyme and stop the reaction.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= DIRECT PCR DIGESTION ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDirect Digestion of PCR Products After Amplification\u003c\/h2\u003e\n\u003cdiv class=\"speedycut-table-wrapper\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eVolume\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePCR reaction mixture\u003c\/td\u003e\n\u003ctd\u003e10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd\u003e7 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10X SpeedyOne Buffer\u003c\/td\u003e\n\u003ctd\u003e2 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpeedyCut PacI\u003c\/td\u003e\n\u003ctd\u003e1–2 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eTotal Reaction Volume\u003c\/th\u003e\n\u003ctd\u003e20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003eCombine the PCR reaction mixture with nuclease-free water, SpeedyOne Buffer, and SpeedyCut PacI.\u003c\/li\u003e\n\u003cli\u003eMix gently and briefly spin down.\u003c\/li\u003e\n\u003cli\u003eIncubate at 37°C for 15–30 minutes.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= DOUBLE DIGESTION ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDouble and Multiple Digestion Guidelines\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse 1 μL of each selected enzyme and enlarge the total reaction volume as needed.\u003c\/li\u003e\n\u003cli\u003eThe combined volume of all enzymes must not exceed one-tenth of the total reaction volume.\u003c\/li\u003e\n\u003cli\u003eWhen the selected restriction enzymes have different optimal reaction temperatures, first incubate with the enzyme that has the lower optimal temperature. Then add the enzyme requiring the higher temperature and continue incubation at the higher temperature.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= QUALITY CONTROL ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003ch3\u003eLong-Incubation and Star Activity Testing\u003c\/h3\u003e\n\u003cp\u003eNo nonspecific degradation caused by contaminating nucleases or star activity was detected after 1 μL of SpeedyCut PacI was incubated with 1 μg of pPacI DNA for 3 hours. The manual notes that star activity may occur with longer incubation.\u003c\/p\u003e\n\u003ch3\u003eCleavage, Ligation, and Recleavage Assay\u003c\/h3\u003e\n\u003cp\u003eDNA substrate was digested using a tenfold enzyme amount and the digested product was recovered. More than 95% of the digested product could be religated using an appropriate amount of T4 DNA ligase at 22°C. After recovery of the ligation product, more than 95% could be cleaved again using the same restriction endonuclease.\u003c\/p\u003e\n\u003ch3\u003eExonuclease Activity Assay\u003c\/h3\u003e\n\u003cp\u003eIn a 50 μL reaction system, less than 0.1% radioactivity was released after a tenfold amount of enzyme was incubated with 1 μg of ultrasonically fragmented, radiolabeled DNA at 37°C for 4 hours.\u003c\/p\u003e\n\u003ch3\u003eNonspecific Endonuclease Activity Assay\u003c\/h3\u003e\n\u003cp\u003eAfter a tenfold amount of enzyme was incubated with 1 μg of supercoiled DNA for 4 hours, the plasmid DNA remained in a supercoiled state when evaluated by agarose gel electrophoresis.\u003c\/p\u003e\n\u003ch3\u003eBlue\/White Cloning Assay\u003c\/h3\u003e\n\u003cp\u003eThe SpeedyCut restriction enzyme series is tested using a blue\/white cloning assay involving digestion, religation, transformation into competent \u003cem\u003eE. coli\u003c\/em\u003e, and colony evaluation. The specified white-colony ratio is less than 1%.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= ENZYME PROPERTIES ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eEnzyme Properties\u003c\/h2\u003e\n\u003cdiv class=\"speedycut-table-wrapper\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePacI Recognition Site\u003c\/th\u003e\n\u003ctd\u003e5′-TTAAT↓TAA-3′\u003cbr\u003e3′-AAT↑TAATT-5′\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eDam Methylation\u003c\/th\u003e\n\u003ctd\u003eThe recognition site does not overlap; no effect is stated.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eDcm Methylation\u003c\/th\u003e\n\u003ctd\u003eThe recognition site does not overlap; no effect is stated.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eCpG Methylation\u003c\/th\u003e\n\u003ctd\u003eThe recognition site does not overlap; no effect is stated.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eThermal Inactivation\u003c\/th\u003e\n\u003ctd\u003e80°C for 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3\u003eNumber of PacI Recognition Sites in Reference DNA\u003c\/h3\u003e\n\u003cdiv class=\"speedycut-table-wrapper\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eDNA\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eNumber of PacI Sites\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eλ DNA\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eφX174\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epBR322\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epUC57\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epUC18\/19\u003c\/td\u003e\n\u003ctd\u003e0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= STORAGE ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore SpeedyCut PacI at \u003cstrong\u003e−20°C\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n  \u003c!-- ========================= DOCUMENTS ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310032_manual.pdf?v=1753776292\" target=\"_blank\"\u003e \u003cstrong\u003eSpeedyCut PacI Product Manual\u003c\/strong\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310032_MSDS.pdf?v=1753776277\" target=\"_blank\"\u003e \u003cstrong\u003eSpeedyCut PacI Safety Data Sheet\u003c\/strong\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= FAQ ========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is SpeedyCut PacI?\u003c\/summary\u003e\n\u003cp\u003eSpeedyCut PacI is a fast PacI restriction enzyme used for DNA restriction digestion. It recognizes the sequence 5′-TTAATTAA-3′ and is supplied with 10X SpeedyOne Buffer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the PacI recognition sequence?\u003c\/summary\u003e\n\u003cp\u003eThe PacI recognition sequence is 5′-TTAATTAA-3′. SpeedyCut PacI cleaves the sequence as 5′-TTAAT↓TAA-3′ and 3′-AAT↑TAATT-5′.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does SpeedyCut PacI digestion take?\u003c\/summary\u003e\n\u003cp\u003eThe recommended digestion time is 15 minutes for plasmid DNA, 15–30 minutes for PCR products, and 30–60 minutes for genomic DNA at 37°C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat reaction buffer is used with SpeedyCut PacI?\u003c\/summary\u003e\n\u003cp\u003eSpeedyCut PacI is used with 1X SpeedyOne Buffer prepared from the supplied 10X SpeedyOne Buffer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan SpeedyCut PacI digest PCR products directly after amplification?\u003c\/summary\u003e\n\u003cp\u003eYes. The recommended 20 μL direct-digestion reaction contains 10 μL PCR reaction mixture, 7 μL nuclease-free water, 2 μL 10X SpeedyOne Buffer, and 1–2 μL SpeedyCut PacI. The reaction is incubated at 37°C for 15–30 minutes.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan SpeedyCut PacI be used for plasmid DNA digestion?\u003c\/summary\u003e\n\u003cp\u003eYes. SpeedyCut PacI is suitable for plasmid DNA digestion. The recommended plasmid digestion time is 15 minutes at 37°C in 1X SpeedyOne Buffer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan SpeedyCut PacI be used for genomic DNA digestion?\u003c\/summary\u003e\n\u003cp\u003eYes. SpeedyCut PacI is suitable for genomic DNA digestion. The recommended incubation time for genomic DNA is 30–60 minutes at 37°C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan SpeedyCut PacI be used in a double digestion?\u003c\/summary\u003e\n\u003cp\u003eYes. SpeedyCut PacI may be combined with other compatible SpeedyCut enzymes in the SpeedyOne Buffer system. The combined enzyme volume must not exceed one-tenth of the total reaction volume.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow is SpeedyCut PacI heat-inactivated?\u003c\/summary\u003e\n\u003cp\u003eIncubate the completed digestion reaction at 80°C for 20 minutes to inactivate SpeedyCut PacI.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes Dam, Dcm, or CpG methylation affect PacI digestion?\u003c\/summary\u003e\n\u003cp\u003eAccording to the product manual, the PacI recognition site does not overlap with Dam, Dcm, or CpG methylation sites, and no effect is stated for these methylation types.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat quality control assays are performed for SpeedyCut PacI?\u003c\/summary\u003e\n\u003cp\u003eQuality control includes long-incubation and star activity testing, cleavage-ligation-recleavage testing, exonuclease activity testing, nonspecific endonuclease activity testing, and a blue\/white cloning assay.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should SpeedyCut PacI be stored?\u003c\/summary\u003e\n\u003cp\u003eSpeedyCut PacI should be stored at −20°C.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs SpeedyCut PacI intended for diagnostic use?\u003c\/summary\u003e\n\u003cp\u003eNo. This product is intended for scientific research and in vitro research use only. It is not tested or intended for diagnostic, therapeutic, food, cosmetic, household, human, or animal administration applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- ========================= RUO ========================= --\u003e\n\u003csection\u003e\n\u003cp class=\"speedycut-note\"\u003e\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e This product is designed for scientific research and in vitro use only. It is not tested or intended for diagnostic or therapeutic procedures, administration to humans or animals, or use as food, cosmetics, or household products.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c!-- ========================= PRODUCT SCHEMA ========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- ========================= FAQ SCHEMA ========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981475509,"sku":"P310032-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-psti","title":"SpeedyCut PstI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PstI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CTGCA↓G-…3' 3'…-G↑ACGTC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310033_manual.pdf?v=1753776420\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310033_MSDS.pdf?v=1753776417\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981442741,"sku":"P310033-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-saci","title":"SpeedyCut SacI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SacI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GAGCT↓C-…3' 3'…-C↑TCGAG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310034_manual.pdf?v=1753776507\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310034_MSDS.pdf?v=1753776529\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981409973,"sku":"P310034-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sali","title":"SpeedyCut SalI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SalI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓TCGAC-…3' 3'…-CAGCT↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310035_manual.pdf?v=1753776580\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310035_MSDS.pdf?v=1753776646\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981377205,"sku":"P310035-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sacii","title":"SpeedyCut SacII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SacII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCGC↓GG-…3' 3'…-GG↑CGCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg pScaII DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310036_manual.pdf?v=1753776738\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310036_MSDS.pdf?v=1753776740\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"60RXNS","offer_id":52400981344437,"sku":"P310036-60RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-spei","title":"SpeedyCut SpeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SpeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CTAGT-…3' 3'…-TGATC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg pUC19-SpeI DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310037_manual.pdf?v=1753776855\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310037_MSDS.pdf?v=1753776839\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981311669,"sku":"P310037-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sbfi","title":"SpeedyCut SbfI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SbfI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCTGCA↓GG-…3' 3'…-GG↑ACGTCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310038_manual.pdf?v=1753776934\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310038_MSDS.pdf?v=1753776927\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981278901,"sku":"P310038-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-smai","title":"SpeedyCut SmaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SmaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCC↓GGG-…3' 3'…-GGG↑CCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 25°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310039_manual.pdf?v=1753777020\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310039_MSDS.pdf?v=1753777015\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981246133,"sku":"P310039-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sphi","title":"SpeedyCut SphI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SphI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GCATG↓C-…3' 3'…-C↑GTACG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310040_manual.pdf?v=1753777116\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310040_MSDS.pdf?v=1753777114\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981213365,"sku":"P310040-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sspi","title":"SpeedyCut SspI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SspI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AAT↓ATT-…3' 3'…-TTA↑TAA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310041_manual.pdf?v=1753777203\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310041_MSDS.pdf?v=1753777201\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"60RXNS","offer_id":52400981180597,"sku":"P310041-60RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-stui","title":"SpeedyCut StuI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut StuI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AGG↓CCT-…3' 3'…-TCC↑GGA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Cleavage is blocked when sequences overlap; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310042_manual.pdf?v=1753777284\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310042_MSDS.pdf?v=1753777283\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981147829,"sku":"P310042-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-taqi","title":"SpeedyCut TaqI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut TaqI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-T↓CGA-…3' 3'…-AGC↑T-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e65°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 65°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect;CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310043_manual.pdf?v=1753778396\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310043_MSDS.pdf?v=1753778395\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981115061,"sku":"P310043-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-xbai","title":"SpeedyCut XbaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut XbaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-T↓CTAGA-…3' 3'…-AGATC↑T-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect;CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310044_manual.pdf?v=1753778727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310044_MSDS.pdf?v=1753778727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981082293,"sku":"P310044-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-xhoi","title":"SpeedyCut XhoI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut XhoI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓TCGAG-…3' 3'…-GAGCT↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310045_manual.pdf?v=1753778817\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310045_MSDS.pdf?v=1753778819\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981049525,"sku":"P310045-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-avrii","title":"SpeedyCut AvrII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AvrII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CTAGG…-3' 3'…-GGATC↑C…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310046_manual.pdf?v=1753779041\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310046_MSDS.pdf?v=1753779046\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981016757,"sku":"P310046-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/20250723_-27_0609105e-7366-4faf-b3f7-359ee13275e2.jpg?v=1754287296","url":"https:\/\/biofargo.com\/collections\/speedycut.oembed?page=6","provider":"Biofargo","version":"1.0","type":"link"}