{"title":"RT-PCR \u0026 cDNA Reagents","description":"","products":[{"product_id":"thermophilic-reverse-transcriptase-cat-rt-pcr-140","title":"Thermophilic Reverse Transcriptase-Genetic Tools","description":"\u003ch2\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis reverse transcriptase or RNA-dependent DNA polymerase is used for real-time fluorescent RT-PCR with either SYBR Green dye or \u003c\/span\u003e\u003cspan\u003eTaqMan probe\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe reverse transcriptase is a thermophilic type A polymerase \u003cspan\u003e(US patent pending) with optimal temperatures at 60-62°C (User Manual Tables 1-2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe enzyme efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤\u003cem\u003e1 unit per 20μL of singleplex reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe enzyme reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 4)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eIt is particularly suitable to one-step\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ereal-time qualitative and quantitative RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/140-UM_RTase_combined_07282023.pdf?v=1704698048\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"200U \/ Taq-Standard-SYBR-Green","offer_id":42917497995445,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"200U \/ Taq-Fast-SYBR-Green","offer_id":42918688784565,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"200U \/ Taq-Probe","offer_id":42918688817333,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Standard-SYBR-Green","offer_id":42917498093749,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Fast-SYBR-Green","offer_id":42918688981173,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Probe","offer_id":42918689013941,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Standard-SYBR-Green","offer_id":42917498192053,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Fast-SYBR-Green","offer_id":42918689177781,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Probe","offer_id":42918689210549,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/140-2500U-B.jpg?v=1732884633"},{"product_id":"copy-of-taq-probe-polymerase-cat-rt-pcr-145","title":"Taq-Fast Polymerase-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e \u003cstrong\u003eIntended use\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThis DNA-dependent DNA polymerase is used real-time PCR and RT-PCR amplification with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eIt is used together with Thermophilic Reverse Transcriptase (Cat 140) for RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast polymerase extends more than 300 bases with short PCR cycling program. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 5x buffer has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e  \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-UM_Taq-Fast_polymerase_07282023.pdf?v=1704699580\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"500U","offer_id":42920672854197,"sku":"148-500","price":103.0,"currency_code":"USD","in_stock":true},{"title":"2500U","offer_id":42920672952501,"sku":"148-2500","price":474.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-500U-B.jpg?v=1732884325"},{"product_id":"1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-146","title":"1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes complementary DNA strands on RNA templates from gene-specific primers,\u003cem\u003e ≥1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704700733\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X1mL \/ No ROX","offer_id":43079388856501,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ Low ROX","offer_id":43079388922037,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ High ROX","offer_id":43079388954805,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ No ROX","offer_id":43079388987573,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ Low ROX","offer_id":43079389020341,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ High ROX","offer_id":43079389053109,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079389085877,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079389118645,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079389151413,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-10ml.jpg?v=1732863909"},{"product_id":"1-step-2x-fast-rt-pcr-master-mix-sybr-green-cat-rt-pcr-147","title":"1-Step 2X Fast RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step Fast 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-UM_Fast_Master_Mix_SYBR_Green_07282023.pdf?v=1704701048\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079401439413,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079401472181,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079401504949,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079401537717,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079401570485,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079401603253,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079401636021,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079401668789,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079401701557,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-2x1mL.jpg?v=1732863901"},{"product_id":"1-step-2x-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit is designed for singleplex RT-PCR with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe concentrations of the primers and probe are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe preferable PCR product size is ≤150bp.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-UM_Master_Mix_TaqMan_07282023.pdf?v=1704700000\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079478214837,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079478280373,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079478313141,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079478345909,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079478378677,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079478411445,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079478444213,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079478476981,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079478509749,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-10ml.jpg?v=1732863923"},{"product_id":"1-step-2x-super-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-149","title":"1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eReverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, with ≥1 unit per 20μL of reaction.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase consistently reversely transcribes single-digit copies of target RNA molecules.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains Super\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704701307\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414918299829,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414918332597,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414918365365,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414918398133,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414918430901,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414918463669,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414918496437,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414918529205,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414918561973,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-2x1mL.jpg?v=1732863930"},{"product_id":"1-step-2x-rt-pcr-master-mix-sybr-green-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X \u003cem\u003eFast\u003c\/em\u003e RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA polymerase for PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-UM_Master_Mix_SYBR_Green_10232023.pdf?v=1704700356\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414936191157,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414936223925,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414936256693,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414936289461,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414936322229,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414936354997,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414936387765,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414936420533,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414936453301,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-2x1mL.jpg?v=1732863916"},{"product_id":"m-mulv-reverse-transcriptase-kleanab","title":"M-MuLV Reverse Transcriptase-KleanAB","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Table SOP (Embedded)\n     ========================== *\/\n  .biofargo-wrap .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n    margin-top:15px;\n  }\n  .biofargo-wrap table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:640px;\n    background:#fff;\n  }\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td{\n    border:1px solid #e9ecef;\n    padding:15px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  .biofargo-wrap table.biofargo-table thead th{\n    background:#f2f2f2;\n    color:#000;\n    font-weight:700;\n    text-align:left;\n  }\n  .biofargo-wrap table.biofargo-table tbody td:first-child{\n    background:#f8f9fa;\n    font-weight:600;\n    width:30%;\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Description\n    \u003c\/h2\u003e\n    \u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n      M-MuLV reverse transcriptase (RT) is a recombinant M-MuLV RT. It differs from M-MuLV RT in terms of structure and catalytic performance. The enzyme has RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of avian myeloblastomavirus (AMV) reverse transcriptase RNase H Minus and acts in a wide range of temperatures (42~55°C).\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Specification\n    \u003c\/h2\u003e\n\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eM-MuLV Reverse Transcriptase\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eReaction Buffer\u003c\/td\u003e\n            \u003ctd\u003e5X Reaction Buffer\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eUnit Definition\u003c\/td\u003e\n            \u003ctd\u003eOne unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol dTMP into a polynucleotide fraction in 10 min at 37°C.\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eThermal Inactivation\u003c\/td\u003e\n            \u003ctd\u003eHeat at 70 °C for 10 min to lose activity.\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eConcentration\u003c\/td\u003e\n            \u003ctd\u003e200 U\/μl\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n            \u003ctd style=\"color:#666;font-size:13px;\"\u003e50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v\/v) Triton X-100, 50% (v\/v) glycerin.\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Features\n    \u003c\/h2\u003e\n    \u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n      \u003cli style=\"margin-bottom: 0; padding-left: 5px;\"\u003eEfficient synthesis of full-length first-strand cDNA up to 13 kb, with activity up to 50°C at 42°C and addition of modified nucleotides\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Applications\n    \u003c\/h2\u003e\n\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eFirst-strand cDNA synthesis for RT-PCR and real-time RT-PCR\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003esynthesis of cDNA for cloning and expression studies\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003ePreparation of labeled cDNA probes for microarray research\u003c\/li\u003e\n      \u003c\/ul\u003e\n\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003eDNA marking\u003c\/li\u003e\n        \u003cli style=\"margin-bottom: 8px;\"\u003ePrimer extension method for RNA analysis\u003c\/li\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px;\"\u003e\n    \u003cdiv style=\"display:flex; flex-wrap:wrap; gap:40px;\"\u003e\n\n      \u003cdiv style=\"flex:1; min-width:280px;\"\u003e\n        \u003ch2 style=\"font-size:20px; color:#0056b3; margin-top:0; margin-bottom:15px;\"\u003eComponents\u003c\/h2\u003e\n        \u003cdiv style=\"padding:15px; border:1px solid #e0e0e0; background-color:#fff; font-size:14px;\"\u003e\n          M-MuLV Reverse Transcriptase (200U\/ul), 5X Reaction Buffer\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv style=\"flex:1; min-width:280px;\"\u003e\n        \u003ch2 style=\"font-size:20px; color:#0056b3; margin-top:0; margin-bottom:15px;\"\u003eStorage\u003c\/h2\u003e\n        \u003cdiv style=\"padding:15px; border:1px solid #ffccd5; background-color:#fff5f5; color:#c53030; font-weight:bold; font-size:18px; border-radius:4px;\"\u003e\n          -20°C\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Sangon","offers":[{"title":"2 KU","offer_id":43839074795701,"sku":"P310143-0002","price":26.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310143_1.jpg?v=1732876483"},{"product_id":"m-mulv-reverse-transcriptase","title":"M-MuLV Reverse Transcriptase","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e2 KU\/10 KU\/5 X 10 KU\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eM-MuLV Reverse Transcriptase\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFirst-strand cDNA synthesis for RT-PCR and real-time RT-PCR; synthesis of cDNA for cloning and expression studies; Preparation of labeled cDNA probes for microarray research; DNA marking; Primer extension method for RNA analysis\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eM-MuLV reverse transcriptase (RT) is a recombinant M-MuLV RT. It differs from M-MuLV RT in terms of structure and catalytic performance. The enzyme has RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of avian myeloblastomavirus (AMV) reverse transcriptase RNase H Minus and acts in a wide range of temperatures (42~55°C).\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eEfficient synthesis of full-length first-strand cDNA up to 13 kb, with activity up to 50°C at 42°C and addition of modified nucleotides\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eM-MuLV Reverse Transcriptase (200U\/ul), 5X Reaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5X Reaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOne unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol dTMP into a polynucleotide fraction in 10 min at 37°C.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 70 °C for 10 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 U\/μl\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v\/v) Triton X-100, 50% (v\/v) glycerin.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eM-MuLV Reverse Transcriptase (200U\/ul), 5X Reaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"2KU","offer_id":52400872521909,"sku":"P310143-2KU","price":26.0,"currency_code":"USD","in_stock":true},{"title":"10KU","offer_id":52400872554677,"sku":"P310143-10KU","price":94.0,"currency_code":"USD","in_stock":true},{"title":"5*10KU","offer_id":52400977150133,"sku":"P310143-50KU","price":401.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_reversetranscriptase.jpg?v=1751472349"},{"product_id":"amv-reverse-transcriptase","title":"AMV Reverse Transcriptase - KleanAB","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant avian myeloblastoma reverse transcriptase expressed by Escherichia coli. AMV RT is a heterodimer consisting of two distinct subunits (alpha 65 KDa and beta 94 KDa subunits). AMV RT has a variety of activities, including RNA and DNA-dependent DNA polymerase activity, DNA-RNA unwinding activity, sequence-specific Mn2+-dependent endonuclease activity, and RNase H (45~60°C) retention activity, so it can reverse transcribe RNA containing a large number of secondary complex structures.\u003c\/p\u003e\n\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003e1 KU\/200 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003eAMV Reverse Transcriptase\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003eAMV RT (10 U\/ul) and 5X AMV RT Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003e5X AMV RT buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003eOne unit is defined as the amount ofenzyme required to catalyze the transfer of 1 nmol of Deoxynucleotidein to acid-precipitable material in 10 minutes at 37°C.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003eHeat at 85 °C for 5 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003e10 U\/μl\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003e200 mM potassium phosphate (pH 7.2 ,4°C), 2 mM DTT, 0.2% (V\/V) Triton X-100 and 50% (V\/V) glycerol.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width:50%; text-align:center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align:center;\"\u003e\u003cstrong\u003eAMV RT (10 U\/ul) and 5X AMV RT Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe product uses all imported raw materials, the properties are more stable.\u003c\/li\u003e\n\u003cli\u003eThe active temperature is 37~58 °C.\u003c\/li\u003e\n\u003cli\u003eThe reverse transcription temperature can reach 60 °C.\u003c\/li\u003e\n\u003cli\u003eThe full-length first-strand cDNA can be efficiently prepared, up to 13kb.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis for RT-PCR and real-time RT-PCR.\u003c\/li\u003e\n\u003cli\u003eIt can increase the reaction temperature and reduce the influence of secondary structure on reverse transcription.\u003c\/li\u003e\n\u003cli\u003eSynthesis of cDNA for cloning and expression research.\u003c\/li\u003e\n\u003cli\u003eSynthetic labeled cDNA probe.\u003c\/li\u003e\n\u003cli\u003ePrimer extension method for RNA analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp style=\"margin-bottom:50px\"\u003e\n\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/P310144_EN_P.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\nhttps:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/P310144_EN_P.pdf\n\u003c\/a\u003e\n\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1KU","offer_id":52400872652981,"sku":"P310144-1KU","price":386.0,"currency_code":"USD","in_stock":true},{"title":"200U","offer_id":52400872685749,"sku":"P310144-200U","price":85.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310144_1_eae1d812-8012-4671-9bd6-8e45ef626322.jpg?v=1732865831"},{"product_id":"extraction-free-1-step-2x-rt-pcr-master-mix-sybr-green-dye","title":"Extraction-Free 1-Step 2X RT-PCR Master Mix, SYBR Green Dye (#142)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142) enables real-time qualitative and quantitative RT-PCR directly from cultured cells without the need for RNA purification. This 2X premixed solution contains all necessary components for one-step RT-PCR except gene-specific primers and RNA templates.\u003c\/p\u003e\n\u003cp\u003eIt streamlines gene expression analysis by allowing direct amplification from crude lysates, eliminating time-consuming and laborious RNA purification steps.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step RT-qPCR workflow: reverse transcription and PCR amplification combined in a single tube.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRobust performance: supports 10 to 100,000 cells per sample, results comparable to purified RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEconomic and efficient: \u003cbr\u003e2 × 1 mL packaging—200 reactions at 20 μL or 400 reactions at 10 μL final volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSYBR Green detection method.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatible with most real-time PCR instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTry Before You Buy — \u003cstrong\u003eRequest a trial sample.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eWhy Choose Biofargo #142?\u003c\/h3\u003e\n\u003cp\u003eOur Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142), is designed as a cost-effective alternative to Thermo Fisher's Cells-to-CT™ 1-Step Power SYBR™ Green Kit.\u003c\/p\u003e\n\u003cp\u003eWith comparable performance in gene expression analysis directly from cell lysates, our kit offers reaction costs as low as $4 per reaction—ideal for both research laboratories and high-throughput applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003eThe 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44624578314421,"sku":"RT-PCR-142-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44624578347189,"sku":"RT-PCR-142-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44624578379957,"sku":"RT-PCR-142-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44624578412725,"sku":"RT-PCR-142-LowRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44624578445493,"sku":"RT-PCR-142-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44624578478261,"sku":"RT-PCR-142-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44624578511029,"sku":"RT-PCR-142-HighRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44624578543797,"sku":"RT-PCR-142-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44624578576565,"sku":"RT-PCR-142-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133256.jpg?v=1732871896"},{"product_id":"extraction-free-rt-pcr-master-mix-taqman-probe-149","title":"1-Step 2X Super multiplex RT-PCR Master Mix-TaqMan Probe (#149)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eThis one-step 2× multiplex RT-PCR Master Mix is designed for real-time qualitative and quantitative amplification with TaqMan probes directly from extraction-free RNA samples. The 2× premixed solution provides all necessary components except gene-specific primers, TaqMan probes, and RNA templates. RT-PCR 149 is optimized for high-GC templates and stem-loop rich regions, and supports multiplex detection of up to two targets, ensuring reliable reverse transcription and amplification of challenging RNA templates.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eKit Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix contains extra more \u003c\/span\u003eredundant amounts of the RTase and DNA polymerase,\u003cspan\u003e specifically designed to overcome endogenous inhibition in extraction-free RNA samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eHarvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHeat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRun RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091748\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44628592099509,"sku":"RT-PCR-149-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44628592132277,"sku":"RT-PCR-149-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44628592165045,"sku":"RT-PCR-149-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44628592197813,"sku":"RT-PCR-149-LowRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44628592230581,"sku":"RT-PCR-149-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44628592263349,"sku":"RT-PCR-149-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44628592296117,"sku":"RT-PCR-149-HighRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44628592328885,"sku":"RT-PCR-149-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44628592361653,"sku":"RT-PCR-149-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a9625ef0e407146a060016379075c19d.jpg?v=1757990155"},{"product_id":"cat-rt-pcr-146-1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe","title":"Extraction-Free 1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe (#146)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers at ≥1 unit per 20 μL of reaction.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe preferable PCR product size is ≤150bp.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e1.Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2.Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e3.Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e4.Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5.Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091746\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2X 1mL","offer_id":44869815828661,"sku":"RT-PCR-146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5X 1mL","offer_id":44869815861429,"sku":"RT-PCR-146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 10mL","offer_id":44869815894197,"sku":"RT-PCR-146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2X 1mL","offer_id":44869815926965,"sku":"RT-PCR-146-LowRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5X 1mL","offer_id":44869815959733,"sku":"RT-PCR-146-LowRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 10mL","offer_id":44869815992501,"sku":"RT-PCR-146-LowRox-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2X 1mL","offer_id":44869816025269,"sku":"RT-PCR-146-HighRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5X 1mL","offer_id":44869816058037,"sku":"RT-PCR-146-HighRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 10mL","offer_id":44869816090805,"sku":"RT-PCR-146-HighRox-1","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133240_1_1.jpg?v=1733237326"},{"product_id":"rt-easytm-ii-master-premix-for-first-strand-cdna-synthesis-for-real-time-pcr","title":"RT EasyTM II (Master Premix for first-strand cDNA synthesis for Real Time PCR)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe company's 2× RT OR-EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.\u003c\/p\u003e\n\u003cp\u003eThe RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. Foregene 2× RT Mix shows extremely high tolerance to alcohol and guanidine salt. The highest tolerance to alcohol in RNA samples is 60%, and the highest tolerance to guanidine salt is 750mM. Even impure RNA can be used for reverse transcription reaction with Foregene 2× RT OR-EasyTM Mix.\u003c\/p\u003e\n\u003cp\u003e2× RT OR-EasyTM Mix is a rapid reverse transcription reagent specially developed for Real Time PCR. This system has high reverse transcription efficiency and does not need to add any primers, and can perform good reverse transcription reactions on a small amount of RNA templates. The unique reaction system makes the RT reaction easier, faster and more efficient. The first strand cDNA synthesis can be completed in 20 minutes. This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003eKit components\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-01022\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-01023\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 T(10μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e800 T (10μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT OR-EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQuick and efficient\u003cbr\u003e\u003c\/strong\u003eEfficient reverse transcription system, it only takes 20 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-sensitivity \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh thermal stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComprehensive\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEven impure RNA samples (alcohol up to 60%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2× RT OR-EasyTM Mix has added optimized reverse transcription primers (Random Primer, Oligo(dT)18 Primer).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReal Time PCR\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUsed directly in Real Time PCR to quantitatively analyze gene expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAnalyze small amounts of RNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt can quickly and accurately analyze small amounts of RNA such as RNA viruses\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComplex system\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReverse transcription of RNA templates with high GC content or complex secondary structure\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTransportation conditions\u003cbr\u003e\u003c\/strong\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4°C.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStorage conditions\u003cbr\u003e\u003c\/strong\u003eRT EasyTM II is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_II_Instruction_Manual-V1.0-EN.docx?v=1752836168\"\u003e\u003cstrong\u003eRT Easy™ II Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200×10μl Reactions","offer_id":51360747716789,"sku":"RT-01022","price":278.0,"currency_code":"USD","in_stock":true},{"title":"800×10μl Reactions","offer_id":51360747749557,"sku":"RT-01023","price":1047.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-010_1_f10f54a7-b7ad-4690-98be-b18fb34f6273.jpg?v=1752810994"},{"product_id":"master-premix-for-first-strand-cdna-synthesis-for-real-time-pcr-with-gdnase","title":"Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRT Easy II(with gDNase) Master Premix system of this kit can also maintain good activity at 50°C. Therefore, it is more advantageous for complex RNA templates and can easily transcribe RNA templates with high GC content and complex secondary structure.\u003c\/p\u003e\n\u003cp\u003eRT EasyTM II (With gDNase) is a reverse transcription system specially developed for Real Time PCR to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.\u003c\/p\u003e\n\u003cp\u003e5× RO-EasyTM Mix contains Foregene Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase with stronger RNA affinity. The optimized system makes the reverse transcription rate faster, and the first strand cDNA synthesis can be completed at 42°C in 15 minutes\u003c\/p\u003e\n\u003ch2\u003eKit components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e5×gDNase Mix\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5×RO-EasyTM Mix\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRNase-Free ddH2O\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInstructions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eComplex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eUsed directly in Real Time PCR to quantitatively analyze gene expression.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIt can quickly and accurately analyze trace RNA such as RNA virus.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReverse transcription of RNA templates with high GC content or complex secondary structure.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThe kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_II_With_gDNase_Instruction_Manual-V1.0.docx?v=1752835294\"\u003e\u003cstrong\u003eRT Easy™ II (With gDNase) Instruction Manual-V1.0\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"100×20μl Reactions","offer_id":51360760627381,"sku":"RT-01032","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-010_4_40340e9a-2557-4ad4-9741-6b1d6ea540c8.jpg?v=1752812181"},{"product_id":"rt-pcr-easytm-ione-step","title":"RT-PCR EasyTM I(One Step)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eForegene One-Step RT-PCR EasyTM series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, which simplifies the experimental steps, optimizes the experimental program, and improves the experiment efficiency.\u003c\/p\u003e\n\u003cp\u003eRT-PCR EasyTM I(One Step) is a one-step RT-PCR kit specially developed by Foregene, which realizes continuous operation from RT to PCR in the same tube. The operation is simple and fast, which can effectively reduce the pollution during the experiment. 2×RT-PCR EasyTM Mix has strong tolerance, thermal stability, high efficiency and specificity for amplification.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe one-step kit enables reverse transcription and PCR to be carried out in the same tube. It only needs to add template RNA, specific PCR primers and RNase-Free ddH2O.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReal-time quantitative analysis of RNA can be carried out quickly and accurately.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003eKit components\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02011\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02012\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100T (20μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500T (20μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT-PCR EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit component information\u003c\/h2\u003e\n\u003cp\u003e2× RT-PCR EasyTM Mix:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase,dNTPs, Mg2+,reaction buffer, optimizer and stabilizer, etc.\u003c\/p\u003e\n\u003ch2\u003ePrecautions: (Please read the precautions carefully before using the kit)\u003c\/h2\u003e\n\u003cp\u003eFor templates, it is recommended to use RNA extracted from fresh samples or stored at -80℃ (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003cp\u003eIn order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn. Before use, put the 2×RT-PCR EasyTM Mix on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and the specificity of PCR amplification.\u003c\/p\u003e\n\u003ch2\u003eTransport and storage conditions\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.Transportation conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Storage conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003ch2\u003eTemplate RNA concentration\u003c\/h2\u003e\n\u003cp\u003eRT-PCR EasyTM I(One Step):(0.1pg-1μg total RNA)\/20μl system\u003c\/p\u003e\n\u003ch2\u003eOperation guide\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eA: Preparation of materials and reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Prepare the prepared RNA template (it is recommended to use Foregene Total RNA Isolation Kit series kits to extract and purify RNA), specific primers (10μM) and related consumables and instruments.\u003c\/p\u003e\n\u003cp\u003eNote: Please ensure the integrity of RNA and try to use RNA extracted from fresh samples.\u003c\/p\u003e\n\u003cp\u003e2. Place 2× RT-PCR EasyTM Mix and RNase-Free ddH2O on an ice bath to let it melt naturally, and flick the tube wall to mix well for later use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB: RT-PCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2×RT-PCR EasyTM Mix is convenient and quick to use, avoiding pollution during operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2×RT-PCR EasyTM Mix solution), add appropriate amount of RNA template and specific primers, and add RNase-Free ddH2O to make up the volume. Refer to Table 1 below for the specific RT-PCR reaction system preparation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 1:RT-PCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-PCR system additions\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAmount\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFinal Concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT-PCR EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eForward Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eReverse Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTemplate(RNA)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eXμl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.1pg-1μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNase-FreeddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e(9-X)μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e20μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1*: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.\u003c\/p\u003e\n\u003cp\u003eNote: Forward Primer and Reverse Primer are the specific primers for the target gene; 50μl system, please refer to the 20μl system to adjust the reagent dosage proportionally.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eC: RT-PCR reaction program setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAfter preparing the RT-PCR system with reference to the above table, mix gently (you can use pipette tip to blow gently; you can also mix on a vortexer and centrifuge briefly to collect the liquid scattered on the tube wall or tube cap, and place on ice box for later use).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e2. Refer to the RT-PCR reaction program settings (Table 2) to set the temperature and time of the reaction.\u003c\/p\u003e\n\u003cp\u003eNote: In order to ensure the activity of 2×RT-PCR EasyTM Mix and improve its amplification efficiency, it is best to prepare the RT-PCR reaction system after setting the PCR instrument program, so that the reaction program can be entered immediately after the system is prepared.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 2:RT-PCR reaction system setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e42℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10-30min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePredenaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"3\"\u003e30-40\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eDenaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e55-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAnnealing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eXmin (2kb\/min)\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eExtension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eFinal extension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: The above procedure is for reference only. The actual reaction conditions vary depending on the structural conditions of the template, primers, etc. For RNA templates with complex secondary structures, the reaction temperature for the first step of reverse transcription is recommended to be 50°C. In the specific operation, it is necessary to design the optimal reaction conditions, including annealing temperature, extension time, etc. according to the specific conditions of the target fragment size, the base sequence of the amplified fragment, and the GC content and length of the primer.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-PCR_EasyTM_I_One_Step_work_600x600.jpg?v=1752813444\"\u003e\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"100 rxns","offer_id":51360856309941,"sku":"RT-02011","price":643.0,"currency_code":"USD","in_stock":true},{"title":"500 rxns","offer_id":51360856342709,"sku":"RT-02012","price":2680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-02011RT-PCREasyTMI_OneStep_3.jpg?v=1752812635"},{"product_id":"rt-pcr-easytm-iitwo-step","title":"RT-PCR EasyTM II(Two Step)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRT-PCR EasyTM II(Two Step) contains all the reagents for two-step RT-PCR, combining RT reaction and PCR reaction in the same kit, providing optimized RT Mix and PCR Mix. The unique RT Mix can synthesize high-quality first-strand cDNA easily and efficiently. 2× PCR EasyTM Mix Plus has high-efficiency and specific amplification. The organic combination of the two makes the detection of the target gene more sensitive.\u003c\/p\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003eKit components\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02021\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02022\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100T (20μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500T (20μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× PCR EasyTM Mix Plus\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRandom Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eOligo(dT)18 Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReal-time quantitative analysis of RNA can be carried out quickly and accurately.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit component information\u003c\/h2\u003e\n\u003cp\u003e2× RT EasyTM Mix:Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, reaction buffer, optimizer and stabilizer, etc.\u003c\/p\u003e\n\u003cp\u003e2× PCR EasyTM Mix Plus :Foregene Taq DNA Polymerase, dNTPs, Mg2+,reaction buffer, optimizer and stabilizer.\u003c\/p\u003e\n\u003cp\u003ePrecautions: (Please read the precautions carefully before using the kit)\u003c\/p\u003e\n\u003cp\u003eReagents should avoid repeated freezing and thawing, otherwise the performance of the reagents will decrease or become invalid.\u003c\/p\u003e\n\u003cp\u003eFor templates, it is recommended to use RNA extracted from fresh samples or stored at -80℃ (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003cp\u003eIn order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn.\u003c\/p\u003e\n\u003cp\u003eThis kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.\u003c\/p\u003e\n\u003cp\u003eBefore use, put 2×RT EasyTM Mix and 2×PCR EasyTM Mix Plus on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and improve Specificity of PCR amplification.\u003c\/p\u003e\n\u003ch2\u003ePrecautions: (Please read the precautions carefully before using the kit)\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReagents should avoid repeated freezing and thawing, otherwise the performance of the reagents will decrease or become invalid.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor templates, it is recommended to use RNA extracted from fresh samples or stored at -80℃ (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIn order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBefore use, put 2×RT EasyTM Mix and 2×PCR EasyTM Mix Plus on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and improve Specificity of PCR amplification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eTransport and storage conditions\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTransportation conditions\u003cbr\u003e\u003c\/strong\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4 °C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStorage conditions\u003cbr\u003e\u003c\/strong\u003eStored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eTemplate RNA concentration\u003c\/h2\u003e\n\u003cp\u003eRT-PCR EasyTM II(Two Step):(0.1pg-5μg total RNA)\/20μl system\u003c\/p\u003e\n\u003ch2\u003eOperation guide\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eA-1:Preparation of materials and reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Prepare the prepared RNA template (it is recommended to use Foregene Total RNA Isolation Kit series kits to extract and purify RNA), specific primers (10μM) and related consumables and instruments.\u003c\/p\u003e\n\u003cp\u003eNote: Please ensure the integrity of RNA and try to use RNA extracted from fresh samples.\u003c\/p\u003e\n\u003cp\u003e2. Put 2×RT EasyTM Mix and RNase-Free ddH2O on an ice bath to let it melt naturally, and flick the tube wall to mix well for later use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eA-2:RT system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2× RT EasyTM Mix is convenient and quick to use, avoiding pollution during operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, just take half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2×RT EasyTM Mix solution), add RNA template and reverse transcription primers, and add RNase-Free ddH2O to make up the volume to 20μl. The specific RT reaction system preparation can refer to Table 1 below.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 1:RT system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eRT-PCR system additions\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e2× RT EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eRandom Primer(50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e1μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eor Oligo(dT)18 Primer(50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e1μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eor Specific Primer(2μM)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e1μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTemplate(RNA)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\n\u003cp\u003eXμl\u003c\/p\u003e\n\u003cp\u003e(Total RNA:\u0026lt;5μg \/ mRNA:\u0026lt;0.5μg)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eRNase-FreeddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e(9-X)μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e20μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eA-3:RT reaction program setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. After preparing the RT system with reference to the above table, mix gently (you can use a pipette tip to blow gently; you can also mix it on the vortexer and centrifuge it to collect the liquid scattered on the tube wall or tube cap, and place it in Ready to use on ice box).\u003c\/p\u003e\n\u003cp\u003e2. Refer to the RT reaction program settings (Table 2) to set the reaction temperature, time, etc.\u003c\/p\u003e\n\u003cp\u003eNote: In order to ensure the activity of Mix and improve its amplification efficiency, it is best to prepare the RT reaction system after the metal bath reaches the set reaction temperature (reverse transcription temperature 42℃ or 50℃), so that the system can be prepared immediately after the completion of the system preparation. Enter the reaction program. The RT reaction can also be performed on a PCR machine.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 2:RT reaction program setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e42℃ or 50℃\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e20 min\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eRenaturation\u0026amp; RT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e85℃\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eDeactivation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: When using Random primer, the reaction should be preheated at 25℃ for 10min before the first step of the reaction. The above procedure is for reference only. The actual reaction conditions vary depending on the structural conditions of the template, primers, etc. For RNA templates with complex secondary results, the first reaction temperature is recommended to be 50℃.\u003c\/p\u003e\n\u003cp\u003e1. The reaction product can be directly used in subsequent tests, or stored at -20°C for up to a week. For long-term storage, it is recommended to avoid repeated freezing and thawing at -80°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB: PCR identification reaction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB-1:PCR reaction system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUse the cDNA synthesized in step A as a template, and perform the PCR reaction with the 2×PCR EasyTM Mix Plus provided in the kit. Place 2×PCR EasyTM Mix Plus, cDNA, and RNase-Free ddH2O on an ice bath to let it melt naturally, and flick the tube wall to mix until use. For the specific PCR system preparation, refer to Table 3 below.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 3:PCR reaction system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-PCR system additions\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eAmount\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eFinal concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2× PCR EasyTM Mix Plus\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eForward Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eReverse Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemplate(cDNA)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eXμl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1-2μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRNase-FreeddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e(9-X)μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e20μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1*: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB-2:PCR reaction condition setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. After preparing the PCR system with reference to the above table, mix gently (you can use a pipette tip to blow gently; you can also mix it on the vortexer and centrifuge it to collect the liquid scattered on the tube wall or tube cap, and place it in Ready to use on ice box).\u003c\/p\u003e\n\u003cp\u003e2. Refer to the PCR reaction program settings (Table 4) to set the temperature and time of the reaction.\u003c\/p\u003e\n\u003cp\u003eNote: In order to ensure the activity of the PCR Mix and improve its amplification efficiency, it is best to set the PCR conditions on the PCR machine before preparing the PCR system.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 4:PCR reaction condition setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePredenaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"3\"\u003e30-40\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eDenaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e55-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAnnealing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eXmin (2kb\/min) 1*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eExtension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eFinal extension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-PCR_EasyTM_II_Two_Step_work_flow_600x600.jpg?v=1752814820\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"100 rxns","offer_id":51360951697589,"sku":"RT-02021","price":643.0,"currency_code":"USD","in_stock":true},{"title":"500 rxns","offer_id":51360951730357,"sku":"RT-02022","price":2680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-02021RT-PCREasyTMII_TwoStep_3.jpg?v=1752813672"},{"product_id":"rt-qpcr-easytm-i-one-step-sybr-green-i","title":"RT-qPCR EasyTM  I (One Step)-SYBR Green I","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRT-qPCR EasyTM (One Step)-SYBR Green Iuses a unique system to effectively combine RT and Real Time PCR reactions, which can quickly complete the quantitative detection of genes. The product has strong tolerance, high specificity and stability. The product contains the company's unique Foregene HotStar Taq DNA Polymerase, which has the advantages of faster amplification (2Kb\/min), high amplification efficiency, strong specific amplification ability, and low mismatch rate compared with ordinary Taq enzymes. It is used here for Real Time RT-PCR to reduce non-specific amplification and improve the accuracy of PCR.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step kit makes reverse transcription and qPCR two reactions in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH2O.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit can quickly and efficiently quantitatively analyze viral RNA or trace RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRT-qPCR EasyTM (One Step)-SYBR Green I kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for customers to use in different models of quantitative PCR instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Contents\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eKit components\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02111\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-02112\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100T (20μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500T (20μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT-qPCR EasyTM Mix-SYBR\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50× ROX Reference Dye\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml×3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit component information\u003c\/h2\u003e\n\u003cp\u003e2× RT-qPCR EasyTM Mix-SYBR:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase, optimized ratio of dNTPs, SYBR GreenI, Mg2+, stabilizers, enhancers, and optimizers.\u003c\/p\u003e\n\u003cp\u003eROX Reference Dye:it is generally used on Real Time PCR amplifiers of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. The concentration of ROX Reference Dye required by different instruments is different, and the user can add it according to the recommended concentration of the instrument.\u003c\/p\u003e\n\u003cp\u003ePrecautions: (Please read the precautions carefully before using the kit)\u003c\/p\u003e\n\u003cp\u003eReagents should avoid repeated freezing and thawing, otherwise the performance of the reagents will decrease or become invalid.\u003c\/p\u003e\n\u003cp\u003eThe reagents in the kit should be protected from light and stored at -20°C.\u003c\/p\u003e\n\u003cp\u003eIt is recommended to use fresh sample extraction or template RNA stored at -80°C (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003cp\u003eIn order to avoid RNase contamination, please perform the experiment in the RNase-Free space; the pipette tips and PCR tubes used must be RNase-Free; and wear disposable gloves and masks.\u003c\/p\u003e\n\u003cp\u003eThis kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.\u003c\/p\u003e\n\u003cp\u003eBefore use, put 2×RT-qPCR EasyTM Mix-SYBR I on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and increase PCR amplification The specificity.\u003c\/p\u003e\n\u003cp\u003e2×RT-qPCR EasyTM Mix-SYBR contains SYBR Green I, please avoid strong light.\u003c\/p\u003e\n\u003ch2\u003eTransport and storage conditions\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1.Transportation conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4 °C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Storage conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit is stored at -20℃. Store the product in a constant temperature refrigerator at -20℃ immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003cbr\u003e2×RT-qPCR EasyTM Mix-SYBR component contains SYBR Green I, please keep it away from light.\u003c\/p\u003e\n\u003ch2\u003eTemplate RNA concentration\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eRT-qPCR EasyTM (One Step)-SYBR Green I:(0.1pg-100ng total RNA)\/20μl system.\u003c\/p\u003e\n\u003ch2\u003eOperation guide\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eA: Preparation of materials and reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Prepare the prepared RNA template (it is recommended to use Foregene Total RNA Isolation Kit series kits to extract and purify RNA), specific primers (10μM) and related consumables and instruments.\u003c\/p\u003e\n\u003cp\u003eNote: Please ensure the integrity of RNA and try to use RNA extracted from fresh samples.\u003c\/p\u003e\n\u003cp\u003e2. Put 2×RT-qPCR EasyTM Mix-SYBR, RNase-Free ddH2O, and 50×ROX Reference Dye (if necessary) on ice to let it melt naturally, and flick the tube wall to mix until use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB:RT-qPCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2× RT-qPCR EasyTM Mix-SYBR is convenient and quick to use, and avoids pollution during operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2×RT-qPCR EasyTM Mix-SYBR solution), add RNA template and specific primers, and add RNase-Free ddH2O to make up the volume. Refer to Table 1 below for the specific RT-qPCR reaction system preparation\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 1:RT-qPCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-qPCR system additions\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eAmount\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eFinal concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2× RT-qPCR EasyTM Mix-SYBR\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eForward Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eReverse Primer (10μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.5μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.2-0.25μM 1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemplate(RNA)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eXμl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.1pg-100ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50× ROX Reference Dye\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRNase-FreeddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e(9-X)μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e20μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.\u003c\/p\u003e\n\u003cp\u003eNote: Forward Primer and Reverse Primer are specific primers for the target gene.\u003c\/p\u003e\n\u003cp\u003e2: Choose the appropriate final concentration of ROX Reference Dye according to different quantitative PCR instruments. The optimal concentration of ROX Reference Dye for common quantitative PCR machines is shown in the following table:\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eQuantitative PCR instrument\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eROX Reference Dye final concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eABI PRISM 7000\/7300\/7700\/7900HT\/Step One etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e5× (i.e.20μl system, add 2μl 50×ROX Reference Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eABI 7500,7500 Fast, Stratagene Mx3000P, Mx3005P, and Mx4000, etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e1× (i.e.20μl system, add 0.4μl 50×ROX Reference Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eRoche PCR instrument, Bio-Rad PCR instrument, Eppendorf quantitative PCR instrument, etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eNo need to add ROX Reference Dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eC:RT-qPCR reaction system setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAfter preparing the RT-qPCR system with reference to the above table, mix gently (you can use a pipette tip to gently pipette; you can also mix on a vortexer and centrifuge briefly to collect the liquid scattered on the tube wall or tube cap, and place on ice box for later use).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e2. Refer to the RT-qPCR reaction program settings (Table 2) to set the temperature and time of the reaction.\u003c\/p\u003e\n\u003cp\u003eNote: In order to ensure the activity of 2×RT-qPCR EasyTM Mix-SYBR and improve its amplification efficiency, it is best to prepare the RT-qPCR reaction system after setting up the PCR instrument program, so that the reaction program can be entered immediately after the system preparation is completed .\u003c\/p\u003e\n\u003cp\u003e3. In order to get the best qPCR effect, gradient PCR can be used to optimize the reaction conditions for different templates and different primers.\u003c\/p\u003e\n\u003cp\u003eNote: The extension temperature range of Foregene Hotstar Taq DNA Polymerase provided in this kit is: 60-72℃, and the best extension temperature is 72℃.\u003c\/p\u003e\n\u003cp\u003eThe following is an example of RT-qPCR reaction conditions. It is recommended to use a two-step method for PCR reaction. When the template concentration is too low to cause non-specific amplification, low primer Tm value leads to low amplification efficiency or poor amplification curve repeatability, etc., it is recommended to try the three-step method for PCR reaction. Refer to Table 2-1 (two-step method) and Table 2-2 (three-step method) for the setting of RT-qPCR reaction conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 2-1:RT-qPCR reaction program setting(two step)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e42℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10-30min 1*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eReverse Transcription\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePre-denaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003e30-45\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemplate denaturation during cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e60-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20sec 2*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAnnealing\/Extension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm 2-2:RT-qPCR reaction program setting( three step)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e42℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10-30min 1*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eReverse Transcription\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePre-denaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10sec\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e30-45\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemplate denaturation during cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e55-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePrimer annealing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20-30sec 2*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eExtension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1: Reverse transcription time can be adjusted according to experimental needs. General endogenous genes such as β-Actin only need 10 minutes; to detect specific expressed genes, reverse transcription time can be appropriately extended as needed.\u003c\/p\u003e\n\u003cp\u003e2: Set a specific time according to the length of the amplified target fragment. The amplification speed of Foregene HotStar Taq DNA Polymerase is 2kb\/min.\u003c\/p\u003e\n\u003cp\u003eNote: PCR reaction conditions vary depending on the structural conditions of the template, primers, etc. For RNA templates with complex secondary structures, it is recommended to use 42℃ for the first step of reverse transcription. In the specific operation, it is necessary to design the optimal reaction conditions according to the specific conditions such as the size of the target fragment, the base sequence of the amplified fragment and the GC content and length of the primer, including annealing temperature, extension time, etc.\u003c\/p\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-qPCR_EasyTM_I_One_Step_-SYBR_Green_I_work_flow_600x600.jpg?v=1752817236\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003ch2\u003ePrecautions: (Please read the precautions carefully before using the kit)\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReagents should avoid repeated freezing and thawing, otherwise the performance of the reagents will decrease or become invalid.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe reagents in the kit should be protected from light and stored at -20°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIt is recommended to use fresh sample extraction or template RNA stored at -80°C (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIn order to avoid RNase contamination, please perform the experiment in the RNase-Free space; the pipette tips and PCR tubes used must be RNase-Free; and wear disposable gloves and masks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBefore use, put 2×RT-qPCR EasyTM Mix-SYBR I on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and increase PCR amplification The specificity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2×RT-qPCR EasyTM Mix-SYBR contains SYBR Green I, please avoid strong ligh\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-qPCR_Easy_I_One_Step_-SYBR_Green_I_Instruction_Manual-V1.0-EN.docx?v=1752836237\"\u003e\u003cstrong\u003eRT-qPCR Easy™ I (One Step)-SYBR Green I Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"100 rxns","offer_id":51362514993333,"sku":"RT-02111","price":536.0,"currency_code":"USD","in_stock":true},{"title":"500 rxns","offer_id":51362515026101,"sku":"RT-02112","price":2143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-02111RT-qPCREasyTMI_OneStep_-SYBRGreenI_1.jpg?v=1752815007"},{"product_id":"rt-qpcr-easytm-ii-one-step-taqman","title":"RT-qPCR EasyTM  II (One Step)-Taqman","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eForegene One-Step RT-PCR Easy series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, thus the experimental steps simplified, the experimental steps are simplified, the experimental plan is optimized, and the experimental efficiency is improved.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eRT-qPCR EasyTM (One Step)-Taqman uses Foregene HotStar Taq DNA Polymerase. The optimized Taqman qPCR system can quickly and specifically perform real-time RT-qPCR quantitative detection of trace RNA templates.\u003c\/p\u003e\n\u003ch2\u003eTransport and storage conditions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTransport and storage conditions: The entire process is carried out in a low-temperature ice box to ensure that the kit is kept below 4°C.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStorage conditions: The kit is stored at -20℃. Store the product in a constant temperature refrigerator at -20℃ immediately after receipt. If stored properly, the product will not lose any performance during the 1-year validity period.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit component information\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e2× RT-qPCR EasyTM Mix-Taqman: Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase, optimized ratio of dNTPs, Mg2+, stabilizer, enhancer, and optimizer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eROX Reference Dye: Generally used on Real Time PCR thermal cyclers of companies such as ABI, Stratagene, etc., to adjust the difference among PCR tubes caused by PCR sample loading errors. The concentration of ROX Reference Dye required by different instruments is different, and the user can add it according to the recommended concentration of the instrument.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReagents should avoid repeated freezing and thawing; otherwise, their performance will decrease or become invalid.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eu The reagents in the kit should be protected from light and stored at -20°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eu It is recommended to use fresh sample extraction or template RNA stored at -80°C (RNA should avoid repeated freezing and thawing).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eu In order to avoid RNase contamination, please perform the experiment in the RNase-Free space; the pipette tips and PCR tubes used must be RNase-Free; and wear disposable gloves and masks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eu This kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eu Before use, put 2× RT-qPCR EasyTM Mix-Taqman on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and increase PCR amplification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eTemplate RNA concentration\u003c\/h2\u003e\n\u003cp\u003eRT-qPCR EasyTM (One Step)-Taqman:(1 pg-100 ng total RNA)\/20 μl system.\u003c\/p\u003e\n\u003ch2\u003eOperation guide\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eA:Preparation of materials and reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePrepare the prepared RNA template (it is recommended to use Foregene Total RNA Isolation Kit series kits to extract and purify RNA), specific primers (10 μM) and related consumables and instruments.\u003cbr\u003eNote: Please ensure the integrity of RNA and try to use RNA extracted from fresh samples.\u003c\/p\u003e\n\u003cp\u003ePut 2× RT-qPCR EasyTM Mix-Taqman, RNase-Free ddH2O, and 20× ROX Reference Dye (if necessary) on ice to thaw naturally, and flick the tube wall to mix before use.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eB:RT-qPCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2× RT-qPCR EasyTM Mix-Taqman is convenient and quick to use, and avoids pollution during operation and experimental errors caused by multiple preparations of the reaction system to the greatest extent. When using, only half the volume of the reaction system (for example, if the reaction system is 20 μl, take 10 μl 2× RT-qPCR EasyTM Mix-Taqman solution), add appropriate amount of RNA template and specific primers, and add RNase-Free ddH2O to make up the volume. The specific RT-qPCR reaction system preparation can refer to Table 1 below.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1: RT-qPCR system preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-qPCR system additions\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eAmount\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eFinal concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2× Real PCR EasyTM Mix-Taqman\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e10 μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eForward Primer (10 μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.8 μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50-900nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eReverse Primer (10 μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.8 μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50-900nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eProbe (4 μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemplate (RNA)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eX μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.1pg-100ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e20× ROX Reference Dye\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eDNase-Free ddH2O\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e(7.4-X) μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTotal Volume\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e20 μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: Forward Primer and Reverse Primer are specific primers for the target gene. The qPCR system can be adjusted according to experimental needs and PCR model. For the final concentration of most primers, we recommend 400nM. Please adjust the dosage of the specific primer and Probe according to the prepared concentration according to our recommended final concentration. For qPCR with 50μl system, please refer to the 20μl system to adjust the amount of reagents proportionally.\u003c\/p\u003e\n\u003cp\u003e1*:Choose the appropriate final concentration of ROX Reference Dye according to different quantitative PCR instruments. The optimal concentration of ROX Reference Dye for common quantitative PCR machines is shown in the following table:\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eQuantitative PCR instrument\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eROX Reference Dye final concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eABI PRISM7000\/7300\/7700\/7900HT\/Step One, etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e1× (eg. 20 μl system, add 1 μl 20× ROX Reference Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eABI 7500\/7500 Fast and Stratagene Mx3000P\/Mx3005P\/Mx4000, etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e0.5× (eg. 20 μl system, add 0.5 μl 20× ROX Reference Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eRoche PCR instrument, Bio-Rad PCR instrument, Eppendorf quantitative PCR instrument, etc.\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eNo need to add ROX Reference Dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eC:RT-qPCR reaction system setting\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAfter preparing the RT-qPCR system with reference to the above table, mix gently (you can use a pipette tip to gently pipette; you can also mix on a vortexer and centrifuge briefly to collect the liquid scattered on the tube wall or tube cap, and place on ice box for later use).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRefer to the RT-qPCR reaction program settings (Table 2) to set the temperature and time of the reaction.\u003c\/p\u003e\n\u003cp\u003eNote: In order to ensure the activity of 2× RT-qPCR EasyTM Mix-Taqman and improve its amplification efficiency, it is best to prepare the RT-qPCR reaction system after setting up the PCR instrument program, so that the reaction program can be entered immediately after the system preparation is completed.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIn order to get the best qPCR effect, gradient PCR can be used to optimize the reaction conditions for different templates and different primers.\u003c\/p\u003e\n\u003cp\u003eNote: The extension temperature range of Foregene Hotstar Taq DNA Polymerase provided in this kit is: 60-72℃, and the best extension temperature is 72℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe following is an example of RT-qPCR reaction conditions. It is recommended to use a two-step method for PCR reaction. When the template concentration is too low to cause non-specific amplification, low primer Tm value leads to low amplification efficiency or poor amplification curve repeatability, etc., it is recommended to try the three-step method for PCR reaction. Refer to Table 2-1 (two-step method) and Table 2-2 (three-step method) for the setting of RT-qPCR reaction conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2-1: RT-qPCR reaction program setting (two-step method)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e42℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10-30 min 1*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eReverse Transcription\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5 min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePre-denaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10 sec\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e30-45\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemplate denaturation during cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e60-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20 sec 2*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAnnealing\/Extension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2-2: RT-qPCR reaction program setting (three-step method)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eStep\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eContent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e42℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10-30 min 1*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eReverse Transcription\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5 min\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePre-denaturation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"3\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e94-95℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e10 sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\" rowspan=\"3\"\u003e30-45\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eTemplate denaturation during cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e55-65℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20 sec\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePrimer annealing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e72℃\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e20-30 sec 2*\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eExtension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e1*: Reverse transcription time can be adjusted according to experimental needs. General endogenous genes such as β-Actin only need 10 minutes; to detect specific expressed genes, reverse transcription time can be appropriately extended as needed.\u003c\/p\u003e\n\u003cp\u003e2*: Set a specific time according to the length of the amplified target fragment. The amplification speed of Foregene HotStar Taq DNA Polymerase is 2kb\/min\u003c\/p\u003e\n\u003cp\u003eNote: PCR reaction conditions vary depending on the structural conditions of the template, primers, etc. For RNA templates with complex secondary structures, it is recommended to use 42℃ for the first step of reverse transcription. In the specific operation, it is necessary to design the optimal reaction conditions according to the specific conditions such as the size of the target fragment, the base sequence of the amplified fragment and the GC content and length of the primer, including annealing temperature, extension time, etc.\u003c\/p\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-qPCR_EasyTM_II_One_Step_-Taqman_work_flow.jpg?v=1752821589\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2 style=\"text-align: start;\"\u003eDocuments\u003c\/h2\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-qPCR_Easy_II_One_Step_-Taqman_Instruction_Manual-V1.0-EN.docx?v=1752836293\"\u003e\u003cstrong\u003eRT-qPCR Easy™ II (One Step)-Taqman Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"100 rxns","offer_id":51363227238581,"sku":"RT-02131","price":536.0,"currency_code":"USD","in_stock":true},{"title":"500 rxns","offer_id":51363227271349,"sku":"RT-02132","price":2143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-02131RT-qPCREasyTMII_OneStep_-Taqman_3.jpg?v=1752819384"},{"product_id":"rt-easytm-i-master-premix-for-first-strand-cdna-synthesis","title":"RT EasyTM I (Master Premix for first-strand cDNA synthesis)","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eFOREGENE’s 2× RT EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.\u003c\/p\u003e\n\u003cp\u003eThe RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT EasyTM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.\u003c\/p\u003e\n\u003cp\u003eThis product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.\u003c\/p\u003e\n\u003ch2\u003eProduct components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eKit contents\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-01011\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-01012\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e25 times (20μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100 times (20μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2× RT EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.25ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRandom Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eOligo(dT)18 Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eInstructions Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient reverse transcription system, it only takes 25 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEven impure RNA samples (alcohol up to 45%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eConventional RT-PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSynthesis of cDNA for cloning and expression research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAnalysis of the primer extension method of the transcription initiation site\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRACE (rapid amplification of cDNA ends)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLinear RNA amplification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChip mark\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePreparations before operation\u003c\/h2\u003e\n\u003cp\u003eIt is strongly recommended that users read the instructions carefully before using this kit. The RT Easy series kits are simple, convenient and fast to operate. The instructions provide the correct use of the entire kit. Please prepare necessary experimental materials and equipment before use.\u003c\/p\u003e\n\u003ch2\u003eStorage and Shelf life\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4 ℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_I_Instruction_Manual-V1.0-EN.docx?v=1752834757\"\u003e\u003cstrong\u003eRT Easy™ I Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"25× 20μl Reactions","offer_id":51366533857461,"sku":"RT-01011","price":188.0,"currency_code":"USD","in_stock":true},{"title":"100 × 20μl Reactions","offer_id":51366533890229,"sku":"RT-01012","price":643.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RTEasyTMI_MasterPremixforfirst-strandcDNAsynthesis_01.jpg?v=1752835213"}],"url":"https:\/\/biofargo.com\/collections\/rt-pcr-cdna-reagents.oembed?page=3","provider":"Biofargo","version":"1.0","type":"link"}