{"title":"RNA\/DNA Quantitation \u0026 Analysis","description":"\u003cp\u003eRNA\/DNA Quantitation \u0026amp; Analysis kits provide precise and reliable methods for measuring nucleic acid concentrations and assessing quality. These kits are designed for accurate quantification and analysis of RNA and DNA in various research and diagnostic applications.\u003c\/p\u003e","products":[{"product_id":"taq-probe-dna-polymerase","title":"Taq-Probe Polymerase-Genetic Tools","description":"\u003ch2\u003eIntended use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThis DNA-dependent DNA polymerase is specially engineered for real-time PCR and RT-PCR amplification with \u003c\/span\u003eTaqMan probe, singleplex or multiplex.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eIt is used together with Thermophilic Reverse Transcriptate (Cat 140) for RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe polymerase is specially engineered for TaqMan probe, its increased 5’-3’ exonuclease activity generating S-shaped curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 5x buffer has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/145-UM_Taq-Probe_polymerase_10022023.pdf?v=1704699039\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"250 rxns","offer_id":42920662991029,"sku":"145-250","price":124.0,"currency_code":"USD","in_stock":true},{"title":"1250 rxns","offer_id":42920663285941,"sku":"145-1250","price":581.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/145-500U-C.jpg?v=1732884330"},{"product_id":"genetic-tools-taq-fast-polymerase-148","title":"Taq-Fast Polymerase-Genetic Tools","description":"\u003ch2\u003eIntended use\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThis DNA-dependent DNA polymerase is used real-time PCR and RT-PCR amplification with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eIt is used together with Thermophilic Reverse Transcriptase (Cat 140) for RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast polymerase extends more than 300 bases with short PCR cycling program. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 5x buffer has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-UM_Taq-Fast_polymerase_07282023.pdf?v=1704699580\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"500U","offer_id":42920672854197,"sku":"148-500","price":103.0,"currency_code":"USD","in_stock":true},{"title":"2500U","offer_id":42920672952501,"sku":"148-2500","price":474.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-500U-B.jpg?v=1732884325"},{"product_id":"1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-146","title":"1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes complementary DNA strands on RNA templates from gene-specific primers,\u003cem\u003e ≥1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704700733\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X1mL \/ No ROX","offer_id":43079388856501,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ Low ROX","offer_id":43079388922037,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ High ROX","offer_id":43079388954805,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ No ROX","offer_id":43079388987573,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ Low ROX","offer_id":43079389020341,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ High ROX","offer_id":43079389053109,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079389085877,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079389118645,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079389151413,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-10ml.jpg?v=1732863909"},{"product_id":"1-step-2x-fast-rt-pcr-master-mix-sybr-green-cat-rt-pcr-147","title":"1-Step 2X Fast RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step Fast 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-UM_Fast_Master_Mix_SYBR_Green_07282023.pdf?v=1704701048\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079401439413,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079401472181,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079401504949,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079401537717,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079401570485,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079401603253,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079401636021,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079401668789,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079401701557,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-2x1mL.jpg?v=1732863901"},{"product_id":"1-step-2x-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit is designed for singleplex RT-PCR with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe concentrations of the primers and probe are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe preferable PCR product size is ≤150bp.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-UM_Master_Mix_TaqMan_07282023.pdf?v=1704700000\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079478214837,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079478280373,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079478313141,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079478345909,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079478378677,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079478411445,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079478444213,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079478476981,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079478509749,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-10ml.jpg?v=1732863923"},{"product_id":"1-step-2x-super-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-149","title":"1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eReverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, with ≥1 unit per 20μL of reaction.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase consistently reversely transcribes single-digit copies of target RNA molecules.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains Super\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704701307\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414918299829,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414918332597,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414918365365,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414918398133,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414918430901,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414918463669,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414918496437,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414918529205,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414918561973,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-2x1mL.jpg?v=1732863930"},{"product_id":"1-step-2x-rt-pcr-master-mix-sybr-green-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X \u003cem\u003eFast\u003c\/em\u003e RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA polymerase for PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-UM_Master_Mix_SYBR_Green_10232023.pdf?v=1704700356\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414936191157,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414936223925,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414936256693,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414936289461,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414936322229,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414936354997,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414936387765,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414936420533,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414936453301,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-2x1mL.jpg?v=1732863916"},{"product_id":"2x-qpcr-master-mix-taqman-probe-cat-qpcr-153","title":"2X qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThis kit uses \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-UM_qPCR_TaqMan_02062023.pdf?v=1704701539\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414982525109,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414982557877,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414982590645,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414982623413,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414982656181,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414982688949,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414982721717,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414982754485,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414982787253,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-10ml.jpg?v=1732864822"},{"product_id":"2x-multiplex-qpcr-master-mix-taqman-probe-cat-qpcr-156","title":"2X Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to 4 targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for multiplex qPCR with TaqMan probes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit uses \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eTwo to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/156-UM_qPCR_Multiplex_TaqMan_02062023.pdf?v=1704701718\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415292149941,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415292182709,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415292215477,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415292248245,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415292281013,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415292313781,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415292346549,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415292379317,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415292412085,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/156-10ml.jpg?v=1732864815"},{"product_id":"2x-fast-qpcr-master-mix-sybr-green-cat-qpcr-157","title":"2X Fast qPCR Master Mix SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Fast qPCR Master Mix is used for real-time qualitative and quantitative qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit contains \u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which can extend more than 300 bases with a short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermo-cycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-UM_qPCR_Fast_SYBR_Green_02062023.pdf?v=1704701908\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415301062837,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415301095605,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415301128373,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415301161141,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415301193909,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415301226677,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415301259445,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415301292213,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415301324981,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-10ml.jpg?v=1732864809"},{"product_id":"2x-super-multiplex-qpcr-master-mix-taqman-probe-cat-qpcr-159","title":"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Multiplex Super qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to four targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eProduct Characterizations\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for multiplex qPCR with TaqMan probes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit uses Super \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and  produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eTwo to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250716159-INSTRUCTION-03012023_1_1.pdf?v=1752655287\" title=\"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003emaster mix\u003c\/div\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415318692021,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415318724789,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415318757557,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415318790325,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415318823093,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415318855861,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415318888629,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415318921397,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415318954165,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/159-10ml.jpg?v=1732864829"},{"product_id":"residual-e-coli-dna-quantitation-kit-2g-shentek™","title":"Residual E. coli DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli DNA Quantitation Kit (2G) is a duplex real‑time PCR (qPCR) assay designed to quantitate residual Escherichia coli host cell DNA in biopharmaceutical samples from in‑process material to final products. The kit provides high sensitivity (fg level), rapid and specific absolute quantitation using an internal positive control (IPC) included in the Primer \u0026amp; Probe mix to monitor PCR performance. The kit includes DNA control, qPCR master mix, DNA dilution buffer and Primer \u0026amp; Probe mix (with IPC). For extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Supplied reagents are sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (in‑process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real‑time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReported sensitivity at the fg level (document states fg level performance)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared serial dilutions: ST0 3000 pg\/µL (stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume (program)\u003c\/td\u003e\r\n \u003ctd\u003eThermal cycling reaction volume set to 30 µL (instrument setting referenced)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15, Annealing\/Extension 60 °C 1:00, 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt thresholds \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct used. Thresholds: E.coli‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC sample recovery: 50%–150%. NTC Ct ≥ 35.00. NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. IPC monitored in VIC channel.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under specified conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, LightCycler 480 (and other compatible real‑time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real‑time PCR:\u003c\/strong\u003e Simultaneous detection of E. coli target and IPC in the same reaction for reliable quantitation and internal reaction control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual E. coli DNA down to the fg level using an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use components:\u003c\/strong\u003e Includes DNA control, qPCR Master Mix, Primer \u0026amp; Probe mix (with IPC) and DNA Dilution Buffer for streamlined workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Built‑in criteria for NTC, NCS and ERC recovery (50%–150%) to support routine QC and method validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument support:\u003c\/strong\u003e Validated for common real‑time PCR instruments (ABI 7500, CFX96, LightCycler 480, SHENTEK‑96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical raw materials, intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and in‑process monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of biologics for host cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and CRO testing workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli Primer \u0026amp; Probe MIX (incl. IPC) (NNC115) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDSs) before use; wear appropriate protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNA‑free consumables; perform UV decontamination of tabletop, pipettes and tubes for 30 minutes and disinfect surfaces with 75% ethanol to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and spin briefly. Protect light‑sensitive reagents (Master MIX and Primer \u0026amp; Probe MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C. Unused DNA Dilution Buffer (DDB) may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (standard curve, NTC, NCS, ERC) and run samples in replicates (recommended triplicates).\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument‑specific settings when adapting the qPCR program; provided cycling parameters and analysis thresholds are validated on ABI 7500 SDS v1.4 and may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity \/ limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports assay performance at the fg level (ultra‑sensitive); exact numeric LOD is not specified in the document and should be determined during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the supplied E. coli DNA Control to generate at least five standard points. Example dilutions in the guide: ST1 300 pg\/µL, ST2 30 pg\/µL, ST3 3 pg\/µL, ST4 0.3 pg\/µL, ST5 0.03 pg\/µL. Include standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC Ct should be ≥ 35.00. NCS Ct should be greater than the mean Ct of the lowest standard and show normal amplification in the VIC (IPC) channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186998108341,"sku":"1101107-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit_2G.jpg?v=1742277494"},{"product_id":"residual-cho-dna-quantitation-kit-2g-shentek™","title":"Residual CHO DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit (2G) is intended to quantitate host-cell line residual DNA from CHO cell lines used in biopharmaceutical production. The kit uses duplex real-time PCR (qPCR) technology to perform rapid, specific and reliable quantitation down to the fg level. An Internal Positive Control (IPC) is included in the CHO Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit provides ready-to-use qPCR Master Mix, CHO Primer \u0026amp; Probe MIX with IPC, a quantified CHO DNA Control for standard curve generation, and DNA Dilution Buffer. For DNA extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Product No.: 1101100-1. Reagents for 100 reactions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (label on kit: Reagents for 100 Reactions).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (serial dilutions)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL (stock), ST1 300 pg\/μL, ST2 30 pg\/μL, ST3 3 pg\/μL, ST4 0.3 pg\/μL, ST5 0.03 pg\/μL, ST6 0.003 pg\/μL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e3000 pg\/μL to 0.003 pg\/μL (nominal dynamic range as provided by standard series).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD (document statement)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims quantitation at the fg level; lowest provided standard is 0.003 pg\/μL (3 fg\/μL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX (15 μL qPCR Master MIX + 5 μL CHO Primer\u0026amp;Probe MIX) + 10 μL sample\/standard\/NTC\/NCS = 30 μL final reaction volume.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extension, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels and dyes\u003c\/td\u003e\r\n \u003ctd\u003eCHO-DNA detector: FAM reporter; IPC detector: VIC reporter; Passive reference: ROX.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected. Threshold: CHO-DNA = 0.05, IPC = 0.1. Automatic baseline recommended. Instrument-specific settings may vary.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptability criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35.00 (or laboratory-specific threshold). NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. ERC recovery should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit components storage conditions\u003c\/td\u003e\r\n \u003ctd\u003eAll listed components stored at -20 °C; protect light where indicated. Kit stable up to 24 months (check label expiration).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ version \/ manufacturer\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1101100-1. User Guide Version: A\/1. Effective date: 01 Jul. 2024. Manufacturer: Biofargo, Inc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR (qPCR):\u003c\/strong\u003e Simultaneous detection of CHO target sequence and an internal positive control (IPC) in the same reaction to monitor assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at the femtogram level; includes a dilution series down to 0.003 pg\/μL (3 fg\/μL) standard.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and CHO Primer \u0026amp; Probe MIX (with IPC) minimize assay setup time and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for common real-time PCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Roche LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes quantified CHO DNA Control for standard curve generation, DNA Dilution Buffer (DDB), and IPC in the primer\/probe mix.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceuticals\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and process development for biologics produced in CHO cells (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control assays in research settings where CHO DNA detection is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer \u0026amp; Probe MIX (including IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five standard curve points; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct acceptance: ≥ 35.00 (or lab-specific threshold). NCS Ct should be greater than mean Ct of lowest standard and display normal VIC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction reference control) recovery must be between 50% and 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation at the femtogram level. The lowest supplied standard is 0.003 pg\/μL (3 fg\/μL); actual limit of detection should be confirmed by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction (15 μL qPCR Master MIX + 5 μL CHO Primer \u0026amp; Probe MIX) and add 10 μL sample or standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which fluorescence channels are used for target and control?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCHO target is detected in the FAM channel; the internal positive control (IPC) is detected in the VIC channel; ROX is used as passive reference when applicable.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35.00 (or a lab-defined threshold). NCS Ct should be higher than the mean Ct of the lowest standard and show VIC amplification. ERC recovery should fall between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187351445685,"sku":"1101100-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit_2G.jpg?v=1742278220"},{"product_id":"residual-hek293-dna-quantitation-kit-3g-shentek™","title":"Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle 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40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px 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display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual HEK293 DNA Quantitation Kit (3G) is designed to quantify residual HEK293 host cell DNA in biopharmaceutical samples at various stages (in‑process to final product). The kit uses duplex real‑time PCR (qPCR) for rapid, specific and reliable quantitation at the fg level. An Internal Positive Control (IPC) is included in the HEK293 Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit supports extraction workflows (refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit, Product No. 1104191) and is supplied as ready‑to‑use master mix and primer\/probe reagents for up to 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDuplex real‑time PCR (qPCR) with target (FAM) and IPC (VIC) channels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX + 10 μL sample\/control = 30 μL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD guidance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit claims quantitation at the fg level. Lowest standard: 0.03 pg\/μL (30 fg\/μL). NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific limit based on validation).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery \/ Accuracy guidance\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (example for ABI7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct. Thresholds: HEK293‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, BioRad CFX96, Roche LightCycler 480 and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims quantitation at the femtogram (fg) level with standard curve down to 0.03 pg\/μL (30 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (IPC) in the Primer \u0026amp; Probe MIX to monitor inhibition and reaction performance in each well.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use reagents:\u003c\/strong\u003e Supplied as qPCR Master MIX and premixed Primer \u0026amp; Probe, minimizing setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated on multiple real‑time PCR platforms (e.g., SHENTEK‑96S, ABI7500, CFX96, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharmaceutical testing:\u003c\/strong\u003e Optimized for residual host cell DNA measurement across in‑process and final product samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in upstream and downstream bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn‑process and final product residual DNA testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer \u0026amp; Probe MIX (Includes IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, lab coat, protective eyewear, mask) and work in nuclease‑free conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and consumables by UV irradiation (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge. Protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Perform laboratory‑specific validation to establish LOD, LOQ and acceptance criteria.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure plate layout includes appropriate standards (at least five concentrations), NTC, NCS and ERC samples; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the claimed sensitivity of the kit and what are the lowest standard concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit claims quantitation at the femtogram (fg) level. The provided standard curve points include ST1 = 300 pg\/μL down to ST5 = 0.03 pg\/μL (30 fg\/μL). Laboratories should perform validation to determine actual LOD\/LOQ in their workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I set up reaction volumes and plate layout?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction and add 10 μL of sample\/control to each well for a total reaction volume of 30 μL. Include at least five standard points, one NTC, one NCS and ERCs as described; triplicate wells are recommended for each sample.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended cycling conditions and analysis settings?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample conditions (ABI7500 SDS v1.4): Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 1:00 with fluorescence read during the 60 °C step. Suggested thresholds: HEK293‑DNA = 0.05; IPC = 0.1; use manual Ct and automatic baseline or adapt per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should I use for ERC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific standard based on validation). The Ct of NCS should be larger than the mean Ct of the lowest standard and show IPC amplification in the VIC channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189158633653,"sku":"1101104-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Quantitation_Kit_3G.jpg?v=1742279848"},{"product_id":"residual-plasmid-dna-quantitation-kit-3g-shentek™","title":"Residual Plasmid DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Plasmid DNA Quantitation Kit (3G) is a duplex real-time PCR (qPCR) kit designed to quantify residual plasmid DNA in gene therapy products (for example, plasmid DNA in lentiviral vector preparations used in CAR‑T manufacturing). The assay targets consensual plasmid sequences (replicon sequences of ColE1\/pMB1\/pBR322\/pUC) and uses a FAM-labeled target probe for quantitative detection and a VIC-labeled Internal Positive Control (IPC) included in the Primer\u0026amp;Probe MIX to monitor reaction performance. The kit provides reagents and a lyophilized plasmid linear DNA control for generation of a standard curve, enabling reliable quantitation down to the 10^2 copies\/µL level. The kit is supplied for 100 reactions and is intended for research use only. Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 1×10^2 copies\/µL (sensitivity reported by manufacturer)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitative dynamic range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd\u003eSerial dilution standard curve covers 4.97×10^8 to 4.97×10^1 copies\/µL (prepared from stock A down through ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total (20 µL qPCR MIX + 10 µL sample\/standard\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Master MIX 15 µL + Plasmid Primer\u0026amp;Probe MIX (incl. IPC) 5 µL = 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extend; fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget probe: FAM (Plasmid-DNA); Internal Positive Control (IPC): VIC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold: Plasmid-DNA (FAM) = 0.05; IPC (VIC) = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NTC Ct should be ≥ (ST5 Ct + 2) or lab-specific; NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable for up to 24 months under recommended storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex qPCR with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (VIC) within the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition or reaction failure.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Reported detection sensitivity at the 10^2 copies\/µL level for trace plasmid DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConsensus plasmid sequence targeting:\u003c\/strong\u003e Assay targets consensual replicon sequences (ColE1\/pMB1\/pBR322\/pUC) to detect a broad range of common plasmid backbones.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents and controls:\u003c\/strong\u003e Includes lyophilized plasmid linear DNA control and pre-mixed Primer\u0026amp;Probe and Master MIX reagents to streamline setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480 and similar platforms).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow for regulated testing environments:\u003c\/strong\u003e Provides recommended standard curve design, plate layouts, and acceptance criteria to support method validation and sample testing.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual plasmid DNA in gene therapy products\u003c\/li\u003e\r\n \u003cli\u003eMonitoring plasmid DNA residue in lentiviral vector preparations (CAR‑T production workflows)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for biologics where plasmid backbone contamination must be measured\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for plasmid removal\/clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid linear DNA Control (NNA016), lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid Primer\u0026amp;Probe MIX (Incl. IPC) (NNC120), 500 µL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023), 850 µL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and observe all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves) when handling reagents and samples.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention tubes and tips to avoid contamination and sample loss.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments to minimize nucleic acid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store all kit components at -20 °C. Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; clear cloudy DDB by warming to 37 °C if necessary.\u003c\/p\u003e\r\n \u003cp\u003e• Include recommended controls (NTC, NCS, ERC, and standard curve points) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the provided plate layouts, thermal profile and analysis settings; adjust thresholds\/criteria only after laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect residual plasmid DNA down to approximately 1×10^2 copies\/µL. Actual limit of detection should be confirmed by the user's in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the lyophilized Plasmid linear DNA Control in 55 µL ddH2O, dilute to the stock concentration indicated on the tube, then perform serial 1:10 dilutions to generate standards A → ST5. Recommended standard points in the manual range from 4.97×10^8 to 4.97×10^1 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret ERC recovery and control criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC (extraction reference control) recovery rates should fall between 50% and 150%. NCS Ct should be larger than the mean Ct of the lowest standard and show VIC amplification; NTC Ct should be ≥ ST5 Ct + 2 (or per your lab validation).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore all kit components at -20 °C and protect light-sensitive reagents from light. Components are stable for up to 24 months when stored under recommended conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189159813301,"sku":"1101111-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Plasmid_DNA_Quantitation_Kit_3G.jpg?v=1742288363"},{"product_id":"residual-e-coli-rna-quantitation-kit-2g-shentek™","title":"Residual E.coli RNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli RNA Quantitation Kit (2G) is designed for quantitation of host cell RNA from Escherichia coli in biopharmaceutical products. The kit employs a duplex reverse transcription quantitative PCR (qRT-PCR) format including a target detector (FAM) and an Internal Positive Control (IPC, VIC) in the E. coli RNA Primer \u0026amp; Probe MIX. The assay provides rapid, specific and reliable quantitation with femtogram-level sensitivity for the target gene. The kit includes One Step qPCR Buffer, One Step Enzyme MIX, Primer \u0026amp; Probe MIX (incl. IPC), E. coli RNA Control and RNase-free water, and is compatible with common real-time PCR instruments.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry\u003c\/td\u003e\r\n \u003ctd\u003eDuplex one-step reverse transcription quantitative PCR (one-step RT-qPCR) with FAM-labeled target and VIC-labeled IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ Limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget detectable at femtogram (fg) level (document states target gene can be determined at the femtogram level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared from 2000 pg\/µL (stock) producing working standards: 20, 2, 0.2, 0.02, 0.002 pg\/µL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 20 µL (15 µL qRT-PCR MIX + 5 µL sample\/standard\/controls)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR mix composition per well\u003c\/td\u003e\r\n \u003ctd\u003eOne Step qPCR Buffer 10 µL, One Step Enzyme MIX 1 µL, E. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) 4 µL — total 15 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 0:30 (1 cycle); 45 cycles of 95 °C 0:10 (denature) and 60 °C 0:40 (anneal\/extend, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold 0.05; Automatic baseline; ROX as passive reference (for ABI 7500), set Standard Curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be at least 2 cycles higher than the lowest standard; ERC recovery acceptance 50%–150%; compare sample IPC Ct to NTC\/NCS IPC Ct to evaluate inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase digestion conditions (for plasmid samples)\u003c\/td\u003e\r\n \u003ctd\u003eIncubate samples with DNase I at 37 °C for 30–60 minutes; recommended DNase I final concentration 0.2–2 U\/µL depending on sample; ensure plasmid sample ≤100 ng\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase inactivation\u003c\/td\u003e\r\n \u003ctd\u003eOptions: (1) SHENTEK® Residual Host Cell RNA Sample Preparation Kit purification; (2) heat inactivation at 75 °C for 10 minutes; (3) other validated methods\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability (kit)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at –20 °C; kit components stable up to 24 months (check label expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex one‑step RT-qPCR:\u003c\/strong\u003e Combined reverse transcription and qPCR in a single reaction for speed and reduced handling\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Target detection at femtogram (fg) level enabling quantitation of trace residual E. coli RNA\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included in Primer \u0026amp; Probe MIX (VIC) to monitor PCR performance and potential inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated buffer, enzyme mix and primer\/probe mix to simplify setup and improve reproducibility\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflow compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, Bio-Rad CFX96)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow for biopharmaceutical samples:\u003c\/strong\u003e Protocols and optional sample prep kit provided for plasmid DNA and protein expression products\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual host cell RNA (HCR) from Escherichia coli in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in plasmid DNA preparations\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in protein expression products derived from E. coli\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and in-process or release testing for downstream product quality control\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Control (NNA011) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) (NNC119) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 100 Reactions | Method: qRT-PCR (duplex one-step reverse transcription quantitative PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive components (buffer and enzyme mix) from light; store all kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice and mix gently; avoid repeated freeze-thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Work in RNase-free conditions: use RNase\/DNase-free consumables, decontaminate surfaces and irradiate equipment with UV for 30 minutes, disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Perform DNase I treatment to remove genomic DNA for plasmid-derived samples; incubate at 37 °C for 30–60 minutes and inactivate DNase I (e.g., 75 °C for 10 minutes or purification).\u003c\/p\u003e\r\n \u003cp\u003e• Ensure final plasmid concentration ≤100 ng\/µL when performing DNase digestion; use DNase I final concentration 0.2–2 U\/µL as appropriate.\u003c\/p\u003e\r\n \u003cp\u003e• Include controls: standards (ST1–ST5), NTC, NCS and ERC; ERC recovery should be within 50%–150% to accept sample results.\u003c\/p\u003e\r\n \u003cp\u003e• If sample IPC Ct is significantly higher than NTC\/NCS IPC Ct, sample inhibition is possible; interpret IPC as reference and consider sample recovery.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect the target gene at the femtogram (fg) level. Use the provided standard curve (typical working standards: 20 to 0.002 pg\/µL) to determine assay sensitivity in your system and matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, Applied Biosystems 7500, Roche LightCycler 480 II and Bio-Rad CFX96. Program settings may require adjustment per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples derived from plasmid DNA be prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePlasmid-derived samples require DNase I digestion to remove genomic DNA (37 °C for 30–60 min). After digestion, inactivate or remove DNase I (e.g., heat 75 °C for 10 min or use the SHENTEK Residual Host Cell RNA Sample Preparation Kit) before qRT-PCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a standard curve (ST1–ST5), no template control (NTC), negative control sample (NCS), and extraction recovery control (ERC). NTC Ct should be ≥2 cycles above the lowest standard; ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189194055861,"sku":"1201201-1","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E.coli_RNA_Quantitation_Kit_2G.jpg?v=1742293356"},{"product_id":"dna-marker100-5000-bp-kleanab","title":"DNA Marker(100~5000 bp)","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n \n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n background:#f9f9f9;\n padding:18px;\n border-radius:10px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\n\/* =========================\n Table Style (Biofargo SOP)\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\"\u003e\n\u003cp\u003eThe DNA molecular weight standard (100-5000 bp) is a ready-to-use product, mixed with 9 single DNA bands, namely 100, 250, 500, 750, 1000, 1500, 2000, 3000, 5000 bp. The brightness of the 500 bp band was enhanced.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 40%;\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eDNA Marker (100–5000 bp)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.01% Bromophenol Blue and 5% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mM Tris-HCl (pH 7.6)；10 mM EDTA；0.01% Bromophenol Blue and 5% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe strip brightness is uniform, the strip shape is clear, and the stability is strong. It can be stored at -20 °C for more than 2 years and can be stored at 4 °C or even room temperature when used frequently. The Marker already contains sample buffer and tracer dye, which can be directly electrophoretic and convenient to use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAgarose gel electrophoresis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\" style=\"background: #fff;\"\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"500ul","offer_id":43839148032181,"sku":"P310145-0500","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310145_1.jpg?v=1732870638"},{"product_id":"2x-taq-pcr-mix-kleanab","title":"2X Taq PCR Mix-KleanAB","description":"\u003cstyle\u003e\n \/* =========================\n Biofargo Table SOP (Embedded)\n - Wrapper scroll + table min-width\n - Strong selector weight via .biofargo-wrap prefix\n - 2-column table default: 640px\n ========================== *\/\n .biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n margin-top:15px;\n }\n .biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px; \/* 2-column table *\/\n background:#fff;\n }\n .biofargo-wrap table.biofargo-table th,\n .biofargo-wrap table.biofargo-table td{\n border:1px solid #e9ecef;\n padding:15px;\n vertical-align:middle;\n white-space:normal;\n word-break:break-word;\n }\n \/* If you ever add a header row later *\/\n .biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n color:#000;\n font-weight:700;\n text-align:left;\n }\n \/* Parameter\/Item column *\/\n .biofargo-wrap table.biofargo-table tbody td:first-child{\n background:#f8f9fa;\n font-weight:600;\n width:30%;\n }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\n \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n Description\n \u003c\/h2\u003e\n \u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n This reagent kit is fast and convenient to use, and can be used for the rapid establishment of ordinary PCR systems and other PCR related experiments. 2X SanTaq PCR Mix includes MgCl2, dNTP, Taq DNA Polymerase, PCR buffer, loading, and PCR enhancer. The optimized PCR Mix enables most PCR reactions to proceed well. The preparation of the PCR reaction system can be completed within a few minutes by adding primers, template DNA, and an appropriate amount of deionized water.\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n Specification\n \u003c\/h2\u003e\n\n \u003cdiv class=\"biofargo-table-wrap\"\u003e\n \u003ctable class=\"biofargo-table\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eFull name\u003c\/td\u003e\n \u003ctd\u003e2X Taq PCR Mix\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eConcentration\u003c\/td\u003e\n \u003ctd\u003e25 μg \/ 500 μl\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eColor\u003c\/td\u003e\n \u003ctd\u003eBlue\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n Features\n \u003c\/h2\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThe product contains bromophenol blue dye, and the PCR product can be directly subjected to electrophoresis.\u003c\/li\u003e\n \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThis product contains excellent stabilizers and can be stored for 3 months at 4 ℃.\u003c\/li\u003e\n \u003cli style=\"margin-bottom: 0; padding-left: 5px;\"\u003eThe PCR product has a 3 '- dA protruding end and can be easily cloned into the T vector.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n Applications\n \u003c\/h2\u003e\n\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n \u003cli style=\"margin-bottom: 0;\"\u003eConventional PCR\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"padding: 30px;\"\u003e\n \u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 40px;\"\u003e\n \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eComponents\u003c\/h2\u003e\n \u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background-color: #fff; font-size: 14px;\"\u003e\n 2X SanTaq PCR Mix (with blue dye)\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eStorage\u003c\/h2\u003e\n \u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background-color: #fff5f5; color: #c53030; font-weight: bold; font-size: 18px; border-radius: 4px;\"\u003e\n -20°C\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Sangon","offers":[{"title":"1ml","offer_id":43839167398069,"sku":"P310149-0100","price":8.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310149_1.jpg?v=1732864843"},{"product_id":"2-phanta-flash-master-mix-dye-plus","title":"2 x Phanta Flash Master Mix (Dye Plus)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e2 × Phanta Flash Master Mix is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec\/kb) while maintaining high fidelity and yield. The amplification error rate of Phanta Flash Super-Fidelity DNA Polymerase is 81-fold lower than that of conventional \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer\/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This product contains tracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHigh fidelity:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e ~81X higher than \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlash amplification: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eExtension speed up to 5 sec\/kb\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eRobust performance: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuperior specificity, sensitivity, and yields\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBroad Compatibility: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuitable for complex samples \u0026amp; GC-rich systems\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eP520-01\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-02\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-03\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e2 × Phanta Flash Master Mix (Dye Plus)\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e5×1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e15×1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExperimental Cases\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2 × Phanta Flash Master Mix (Vazyme#P510), 2 × Phanta Flash Master Mix (Dye Plus) (Vazyme#P520) and other commercially available brands of high-fidelity polymerases were used to perform PCR amplification. The target fragment of 353 bp and 873 bp were amplified at an extension time of 1 sec\/kb, 7140 bp fragment was amplified at an extension time of 4 sec\/kb and 5 sec\/kb, respectively. The results show that all the fragments could be successfully amplified by P510 and P520.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1.png?v=1765791826\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAmplification of fragments ≤10 kb can be achieved within 1 h!\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_2cd7730b-c2f8-4911-b129-4685a4c7e4f7.png?v=1765791869\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Specificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eVazyme#P510, Vazyme#P520 and other commercially available brands of high-fidelity polymerases were used to amplify different length of fragments in accordance to the recommended systems and procedures of respective instructions. The results show that P510 and P520 have higher amplification specificity than other brands of high-fidelity polymerases.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_1d7164bb-8d83-4cfa-8c65-f27b25d4f9f7.png?v=1765791908\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at -30 ~ -15°C and transport at ≤0°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ1: Does the tracking dye in 2 × Phanta Flash Master Mix (Dye Plus) interfere with downstream applications?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA1: The tracking dye (which consists of Bromophenol Blue) is compatible with downstream applications such as fluorescent automated DNA sequencing, ligation, and restriction digestion. For applications that require analysis of PCR products by absorbance or fluorescence excitation, we recommend using colorless versions of the products Phanta Flash Super-Fidelity DNA Polymerase or 2 × Phanta Flash Master Mix or purifying the PCR products prior to analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ2: Can I perform restriction digestion in the Phanta Flash reaction buffer?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA2: Phanta Flash reaction buffer is compatible with restriction digestion directly after PCR. Note that Phanta Flash Super-Fidelity DNA Polymerase is not inactivated during PCR, thus purification of PCR products before restriction digestion is recommended if intact 5'- or 3'- overhangs are needed for cloning.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ3: Can I amplify GC-rich targets with Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA3: Phanta Flash Super-Fidelity DNA Polymerase can amplify targets with high GC content (up to 83% GC) without any additional DNA melting agents. In cases of extremely GC-rich targets (\u0026gt;83% GC), addition of DMSO to final concentration of 5% is recommended.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ4: What are your recommendations when working with long amplicons using Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA4: Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.3 µM may also improve the results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ5: What are your recommendations when calculating annealing temperatures using Phanta Flash Super-Fidelity DNA Polymerase?\u003cbr\u003e\u003c\/strong\u003eA5: Approximately 5°C above Tm of primers, but no more than 68°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Manual_P520_V23.1.pdf?v=1716346848\" type=\"application\/pdf\" target=\"_blank\"\u003eManual_P520 V23.1\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003ePurchase Help\u003c\/h2\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/b8a2a61f1ed241a5ada61e12cf731c35.png?v=1765792300\"\u003e\u003c\/p\u003e\n\u003c!----\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003epcr buffer,master mix\u003c\/div\u003e","brand":"Vazyme","offers":[{"title":"15×1ml","offer_id":44176146596021,"sku":"P520-03","price":897.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml","offer_id":44176146628789,"sku":"P520-02","price":320.0,"currency_code":"USD","in_stock":true},{"title":"1ml","offer_id":44176146661557,"sku":"P520-01","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1780083306473254913.jpg?v=1732864515"},{"product_id":"mag-beads-gst-fusion-protein-purification","title":"Mag-Beads GST Fusion Protein Purification","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n border-left:4px solid #0056b3;\n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n background:#f9f9f9;\n padding:20px;\n border-radius:6px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n border:1px solid #e1e1e1;\n padding:20px;\n border-radius:10px;\n background:#fff;\n}\n\n\/* =========================\n Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n white-space:normal;\n text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n min-width:640px;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n margin:0;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n \u003c!-- Description --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eDescription\u003c\/h2\u003e\n \u003cdiv class=\"desc-box\"\u003e\n \u003cp style=\"margin:0;\"\u003eMag-Beads GST fusion protein purification magnetic beads are a new type of purification material developed for purifying GST labeled fusion proteins. They can directly purify high-purity target proteins from biological samples through a unique magnetic separation method, greatly simplifying the purification process and improving purification efficiency. They are suitable for convenient purification of GST fusion proteins in scientific research and industrial fields.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Specification --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eSpecification\u003c\/h2\u003e\n \u003cdiv class=\"biofargo-table-wrap\"\u003e\n \u003ctable class=\"biofargo-table w-640\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n \u003cth\u003eSpecification\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003ePackage\u003c\/td\u003e\n \u003ctd\u003e10 ML（10%V\/V)\/25 ML（10%V\/V)\/100 ML（10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eFull name\u003c\/td\u003e\n \u003ctd\u003eMag-Beads GST Fusion Protein Purification\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eComposition\u003c\/td\u003e\n \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSubstrate\u003c\/td\u003e\n \u003ctd\u003eMagnetic agarose microspheres\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAverage Particle Size\u003c\/td\u003e\n \u003ctd\u003e0\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eParticle Size\u003c\/td\u003e\n \u003ctd\u003e30 μm~150 μm\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eProtein Binding Ability\u003c\/td\u003e\n \u003ctd\u003e6-10 mg GST fusion protein\/ml (pure magnetic beads)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n \u003ctd\u003e20% ethanol\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eMagnetic Bead Ratio\u003c\/td\u003e\n \u003ctd\u003eMagnetic beads suspended in protective solution with a content of 10% (V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eComponent\u003c\/td\u003e\n \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Features --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eFeatures\u003c\/h2\u003e\n \u003cul\u003e\n \u003cli\u003eDirectly purifying crude protein reduces equipment, manpower, and time costs, and is more suitable for large-scale protein purification production.\u003c\/li\u003e\n \u003cli\u003eThe purity and loading of one-step purification are higher than those of ordinary column chromatography.\u003c\/li\u003e\n \u003cli\u003eGood stability, not affected by flow velocity.\u003c\/li\u003e\n \u003cli\u003eReusable.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- Applications --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eApplications\u003c\/h2\u003e\n \u003cdiv class=\"card\"\u003e\n \u003cp style=\"margin:0;\"\u003eSuitable for convenient purification of GST fusion proteins in scientific research and industrial fields.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Storage --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eStorage\u003c\/h2\u003e\n \u003cdiv class=\"card\"\u003e\n \u003cp style=\"margin:0;\"\u003e2~8°C, do not freeze\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ml(10%V\/V)","offer_id":52400869802165,"sku":"P310135-10ml(10%V\/V)","price":148.0,"currency_code":"USD","in_stock":true},{"title":"25ml(10%V\/V)","offer_id":52400869834933,"sku":"P310135-25ml(10%V\/V)","price":302.0,"currency_code":"USD","in_stock":true},{"title":"100ml(10%V\/V)","offer_id":52400869867701,"sku":"P310135-100ml(10%V\/V)","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_magbeads.png?v=1751472361"},{"product_id":"mag-beads-his-tag-protein-purification","title":"Mag-Beads His-Tag Protein Purification","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n border-left:4px solid #0056b3;\n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n background:#f9f9f9;\n padding:20px;\n border-radius:6px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n border:1px solid #e1e1e1;\n padding:20px;\n border-radius:10px;\n background:#fff;\n}\n\n\/* =========================\n Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n white-space:normal;\n text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n min-width:640px;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n margin:0;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n \u003c!-- Description --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eDescription\u003c\/h2\u003e\n \u003cdiv class=\"desc-box\"\u003e\n \u003cp style=\"margin:0;\"\u003eMag-Beads His Tag protein purification magnetic beads are a new type of purification material developed for purifying His-tag proteins. They can directly purify high-purity target proteins from biological samples through a unique magnetic separation method, greatly simplifying the purification process and improving purification efficiency. They are suitable for convenient purification of His-tag proteins in scientific research and industrial fields.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Specification --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eSpecification\u003c\/h2\u003e\n \u003cdiv class=\"biofargo-table-wrap\"\u003e\n \u003ctable class=\"biofargo-table w-640\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n \u003cth\u003eSpecification\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003ePackage\u003c\/td\u003e\n \u003ctd\u003e10 ML（10%V\/V)\/25 ML（10%V\/V)\/100 ML（10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eFull name\u003c\/td\u003e\n \u003ctd\u003eMag-Beads His-Tag Protein Purification\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eComposition\u003c\/td\u003e\n \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSubstrate\u003c\/td\u003e\n \u003ctd\u003eMagnetic agarose microspheres\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAverage Particle Size\u003c\/td\u003e\n \u003ctd\u003e0\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eParticle Size\u003c\/td\u003e\n \u003ctd\u003e30 μm~150 μm\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eProtein Binding Ability\u003c\/td\u003e\n \u003ctd\u003e\u0026gt;10 mg histidine labeled protein\/ml (pure magnetic beads)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n \u003ctd\u003e20% ethanol\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eMagnetic Bead Ratio\u003c\/td\u003e\n \u003ctd\u003eMagnetic beads suspended in protective solution with a content of 10% (V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eComponent\u003c\/td\u003e\n \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Features --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eFeatures\u003c\/h2\u003e\n \u003cul\u003e\n \u003cli\u003eDirectly purifying crude protein reduces equipment, manpower, and time costs, and is more suitable for large-scale protein purification production.\u003c\/li\u003e\n \u003cli\u003eOne step purification can obtain up to 95% purity of the target protein.\u003c\/li\u003e\n \u003cli\u003eGood stability, not affected by flow velocity.\u003c\/li\u003e\n \u003cli\u003eReusable.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- Applications --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eApplications\u003c\/h2\u003e\n \u003cdiv class=\"card\"\u003e\n \u003cp style=\"margin:0;\"\u003eSuitable for convenient purification of His-tag protein in scientific research and industrial fields.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Storage --\u003e\n \u003cdiv class=\"sec\"\u003e\n \u003ch2\u003eStorage\u003c\/h2\u003e\n \u003cdiv class=\"card\"\u003e\n \u003cp style=\"margin:0;\"\u003e2~8°C, do not freeze\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ml(10%V\/V)","offer_id":52400870097077,"sku":"P310137-10ml(10%V\/V)","price":148.0,"currency_code":"USD","in_stock":true},{"title":"25ml(10%V\/V)","offer_id":52400870129845,"sku":"P310137-25ml(10%V\/V)","price":302.0,"currency_code":"USD","in_stock":true},{"title":"100ml(10%V\/V)","offer_id":52400870162613,"sku":"P310137-100ml(10%V\/V)","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_magbeads.png?v=1751472361"},{"product_id":"dna-marker100-3000-bp","title":"DNA Marker(100-3000 bp)","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.7;\n}\n\n.biofargo-sec{\n margin-bottom:32px;\n}\n\n.biofargo-h2{\n border-left:4px solid #0056b3;\n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-card{\n background:#f9f9f9;\n padding:18px;\n border-radius:10px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\n\/* =========================\n Table Style\n========================== *\/\n\n.biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n}\n\n.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px;\n}\n\n.biofargo-table th,\n.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n}\n\n.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Description --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\"\u003e\n\u003cp\u003eThe DNA molecular weight standard Ladder H1-K (100-3000 bp) is a ready-to-use product, mixed with 14 single DNA bands: 100,200,300,400,500,600,700,800,900,1000,1200,1500,2000,3000 bp.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e300μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eDNA Marker(100~3000 bp)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.033% Bromophenol Blue, 0.008% xylene cyanol FF and 10% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mM Tris-HCl (pH 7.6)；10 mM EDTA；0.033% Bromophenol Blue；0.008% xylene cyanol FF and 10% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003ePurple\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eThe strip brightness is uniform, the strip shape is clear, and the stability is strong.\u003c\/li\u003e\n\u003cli\u003eIt can be stored at -20 °C for more than 2 years and can be stored at 4 °C or even room temperature when used frequently.\u003c\/li\u003e\n\u003cli\u003eThe Marker already contains sample buffer and tracer dye, which can be directly electrophoretic and convenient to use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eAgarose gel electrophoresis or non denatured PAGE gel\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\" style=\"background:#fff;\"\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003ep310146, p310146-300\u003c\/div\u003e\n","brand":"KleanAB","offers":[{"title":"300ul","offer_id":52400977117365,"sku":"P310146-300ul","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_dnamarker.jpg?v=1751472363"},{"product_id":"dna-marker-100-2000bp","title":"DNA Marker (100-2000bp)","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n \n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n background:#f9f9f9;\n padding:18px;\n border-radius:10px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\n\/* =========================\n Table Style (Biofargo SOP)\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\"\u003e\n\u003cp\u003eThe 2K Plus DNA Marker is composed of 8 linear double stranded DNA bands, namely: 100, 250, 500, 750, 1000, 1200, 1500, and 2000 bp, with 1 bp bands displayed as bright bands.\u003c\/p\u003e\n\u003cp\u003eThis product was previously listed under catalog number P310147-0500 and is now updated to P310147-500ul for improved product management.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 40%;\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eDNA Marker (100–2000 bp)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e1 mM Tris-HCl (pH 8.), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% xylene cyanol FF, and 5% Glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mM Tris-HCl (pH 8.), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% xylene cyanol FF, and 5% Glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe strip has uniform brightness, strong stability, and can be directly electrophoretic, making it convenient and fast.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThis product contains sample buffer, which is a ready to use product, and is suitable for the analysis of DNA bands in agarose gel electrophoresis experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\" style=\"background: #fff;\"\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"500ul","offer_id":52400977084597,"sku":"P310147-500ul","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_dnamarker.jpg?v=1751472363"},{"product_id":"dna-marker-50-500bp","title":"DNA Marker (50-500bp)","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n \n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n background:#f9f9f9;\n padding:18px;\n border-radius:10px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\n\/* =========================\n Table Style (Biofargo SOP)\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n -webkit-overflow-scrolling:touch;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\"\u003e\n\u003cp\u003eThe 5bp Plus DNA Marker is composed of 8 linear double stranded DNA bands, namely: 50, 100, 150, 200, 250, 300, 400, and 500 bp, with 250 bp bands displayed as bright bands.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 40%;\"\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePackage\u003c\/td\u003e\n\u003ctd\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003eDNA Marker (50–500 bp)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e1 mM Tris-HCl (pH 8.), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% xylene cyanol FF and 5% Glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e25 μg \/ 5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mM Tris-HCl (pH 8.), 1 mM EDTA, 0.2% Bromophenol Blue, 0.1% xylene cyanol FF, and 5% Glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe strip has uniform brightness, strong stability, and can be directly electrophoretic, making it convenient and fast.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThis product contains sample buffer, which is a ready to use product, and is suitable for the analysis of DNA bands in agarose gel electrophoresis experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\" style=\"background: #fff;\"\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"500ul","offer_id":52400977051829,"sku":"P310148-500ul","price":25.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_dnamarker.jpg?v=1751472363"},{"product_id":"2x-taq-pcr-mix","title":"2X Taq PCR Master Mix (with Dye)","description":"\u003cstyle\u003e\n\/* =========================\n Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n max-width:1000px;\n margin:0 auto;\n padding:10px;\n font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color:#333;\n line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n border-left:4px solid #0056b3;\n padding-left:15px;\n color:#0056b3;\n font-size:20px;\n text-transform:uppercase;\n margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n background:#f9f9f9;\n padding:18px;\n border-radius:10px;\n border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n padding-left:22px;\n}\n\n.biofargo-wrap li{\n margin:8px 0;\n}\n\n\/* =========================\n Table Style\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n overflow-x:auto;\n border:1px solid #eee;\n border-radius:10px;\n padding:6px;\n background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n width:100%;\n border-collapse:collapse;\n font-size:14px;\n min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n border:1px solid #ddd;\n padding:12px;\n vertical-align:middle;\n word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n background:#f2f2f2;\n font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n font-weight:700;\n background:#f8f9fa;\n}\n\n\/* =========================\n Document Button\n========================== *\/\n\n.biofargo-doc-btn{\n display:inline-block;\n padding:10px 18px;\n background:#0056b3;\n color:#fff !important;\n border-radius:6px;\n text-decoration:none;\n font-weight:600;\n}\n\n.biofargo-doc-btn:hover{\n background:#003f85;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Background --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eBackground\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\"\u003e\n\n\u003cp\u003eThe 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays.\u003c\/p\u003e\n\n\u003cp\u003eThe added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eItem\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003e2X Taq PCR Master Mix (with Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage Size\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eConventional PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e2X SanTaq PCR Mix (with bromophenol blue dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eTaq Polymerase Source\u003c\/td\u003e\n\u003ctd\u003eRecombinant, E. coli Expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e25 μg \/ 500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReady-to-Use 2X Formulation\u003c\/strong\u003e\u003cbr\u003e\nPre-mixed with Taq DNA polymerase, MgCl₂, dNTPs, PCR buffer, and enhancers for fast PCR setup.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eDirect Gel Loading\u003c\/strong\u003e\u003cbr\u003e\nContains bromophenol blue dye, enabling PCR products to be loaded directly onto agarose gels.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReliable Amplification Performance\u003c\/strong\u003e\u003cbr\u003e\nOptimized buffer system supports stable and efficient amplification in routine PCR assays.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eTA Cloning Compatible\u003c\/strong\u003e\u003cbr\u003e\nGenerates PCR products with 3′-dA overhangs, facilitating efficient ligation into T-vectors.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eEnhanced Stability\u003c\/strong\u003e\u003cbr\u003e\nFormulated with stabilizers; can be stored at 4 °C for up to 3 months and long-term at −20 °C.\n\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eConventional PCR amplification\u003c\/li\u003e\n\u003cli\u003eGene cloning and insert preparation\u003c\/li\u003e\n\u003cli\u003eGenotyping and mutation analysis\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for DNA sequencing\u003c\/li\u003e\n\u003cli\u003eRoutine molecular biology research workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\" style=\"background:#fff;\"\u003e\n\u003cp\u003eStore the 2X master mix at -20°C.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Documents --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n\n\u003ca class=\"biofargo-doc-btn\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2X_Taq_PCR_Master_Mix_with_Dye.pdf?v=1766022843\" target=\"_blank\"\u003e\n2X Taq PCR Master Mix (with Dye)\n\u003c\/a\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003emaster mix\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"1ml","offer_id":52400976887989,"sku":"P310149-1ml","price":21.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_taqpcrmix.jpg?v=1751472348"},{"product_id":"extraction-free-1-step-2x-rt-pcr-master-mix-sybr-green-dye","title":"Extraction-Free 1-Step 2X RT-PCR Master Mix, SYBR Green Dye (#142)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142) enables real-time qualitative and quantitative RT-PCR directly from cultured cells without the need for RNA purification. This 2X premixed solution contains all necessary components for one-step RT-PCR except gene-specific primers and RNA templates.\u003c\/p\u003e\n\u003cp\u003eIt streamlines gene expression analysis by allowing direct amplification from crude lysates, eliminating time-consuming and laborious RNA purification steps.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step RT-qPCR workflow: reverse transcription and PCR amplification combined in a single tube.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRobust performance: supports 10 to 100,000 cells per sample, results comparable to purified RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEconomic and efficient: \u003cbr\u003e2 × 1 mL packaging—200 reactions at 20 μL or 400 reactions at 10 μL final volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSYBR Green detection method.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatible with most real-time PCR instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTry Before You Buy — \u003cstrong\u003eRequest a trial sample.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg alt=\"Extraction-Free 1-Step RT-PCR workflow from cultured cells including cell washing, 5-minute lysis, 10-minute heat treatment, and direct SYBR Green RT-qPCR analysis\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eWhy Choose Biofargo #142?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOur Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142), is designed as a cost-effective alternative to Thermo Fisher's Cells-to-CT™ 1-Step Power SYBR™ Green Kit.\u003c\/p\u003e\n\u003cp\u003eWith comparable performance in gene expression analysis directly from cell lysates, our kit offers reaction costs as low as $4 per reaction—ideal for both research laboratories and high-throughput applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat nucleotides are included in the 2× RT-PCR Master Mix? Does it contain UTP?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003eThe 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44624578314421,"sku":"RT-PCR-142-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44624578347189,"sku":"RT-PCR-142-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44624578379957,"sku":"RT-PCR-142-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44624578412725,"sku":"RT-PCR-142-LowRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44624578445493,"sku":"RT-PCR-142-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44624578478261,"sku":"RT-PCR-142-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44624578511029,"sku":"RT-PCR-142-HighRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44624578543797,"sku":"RT-PCR-142-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44624578576565,"sku":"RT-PCR-142-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133256.jpg?v=1732871896"},{"product_id":"extraction-free-rt-pcr-master-mix-taqman-probe-149","title":"1-Step 2X Super multiplex RT-PCR Master Mix-TaqMan Probe (#149)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eThis one-step 2× multiplex RT-PCR Master Mix is designed for real-time qualitative and quantitative amplification with TaqMan probes directly from extraction-free RNA samples. The 2× premixed solution provides all necessary components except gene-specific primers, TaqMan probes, and RNA templates. RT-PCR 149 is optimized for high-GC templates and stem-loop rich regions, and supports multiplex detection of up to two targets, ensuring reliable reverse transcription and amplification of challenging RNA templates.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eKit Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix contains extra more \u003c\/span\u003eredundant amounts of the RTase and DNA polymerase,\u003cspan\u003e specifically designed to overcome endogenous inhibition in extraction-free RNA samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eHarvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHeat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRun RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091748\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44628592099509,"sku":"RT-PCR-149-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44628592132277,"sku":"RT-PCR-149-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44628592165045,"sku":"RT-PCR-149-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44628592197813,"sku":"RT-PCR-149-LowRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44628592230581,"sku":"RT-PCR-149-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44628592263349,"sku":"RT-PCR-149-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44628592296117,"sku":"RT-PCR-149-HighRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44628592328885,"sku":"RT-PCR-149-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44628592361653,"sku":"RT-PCR-149-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a9625ef0e407146a060016379075c19d.jpg?v=1757990155"},{"product_id":"cat-rt-pcr-146-1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe","title":"Extraction-Free 1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe (#146)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers at ≥1 unit per 20 μL of reaction.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe preferable PCR product size is ≤150bp.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e1.Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2.Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e3.Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e4.Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5.Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091746\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2X 1mL","offer_id":44869815828661,"sku":"RT-PCR-146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5X 1mL","offer_id":44869815861429,"sku":"RT-PCR-146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 10mL","offer_id":44869815894197,"sku":"RT-PCR-146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2X 1mL","offer_id":44869815926965,"sku":"RT-PCR-146-LowRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5X 1mL","offer_id":44869815959733,"sku":"RT-PCR-146-LowRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 10mL","offer_id":44869815992501,"sku":"RT-PCR-146-LowRox-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2X 1mL","offer_id":44869816025269,"sku":"RT-PCR-146-HighRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5X 1mL","offer_id":44869816058037,"sku":"RT-PCR-146-HighRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 10mL","offer_id":44869816090805,"sku":"RT-PCR-146-HighRox-1","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133240_1_1.jpg?v=1733237326"},{"product_id":"residual-vero-dna-quantitation-kit-shentek™","title":"Residual Vero DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Vero DNA Quantitation Kit is designed for quantitative measurement of residual Vero host cell DNA in biopharmaceutical samples across processing stages, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) with a Vero-specific primer \u0026amp; probe mix to deliver rapid, specific and reliable quantitation down to the femtogram (fg) level. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit provides reagents sufficient for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (Standard curve \/ Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at the femtogram (fg) level (document states femtogram-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST0 (3000 pg\/µL prepared stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL; ST6 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL; Sample\/Standard\/NTC\/NCS addition: 10 µL; Total reaction volume: 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + Vero Primer\u0026amp;Probe MIX 3 µL = 20 µL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector \/ Dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: (none specified)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected; Threshold for Vero-DNA set to 0.02; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ controls\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard; ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycles\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments (examples)\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 (other real-time PCR systems likely compatible after instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed for quantitation down to the femtogram (fg) level for detection of trace residual Vero DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Includes qPCR Reaction Buffer and Vero-specific Primer \u0026amp; Probe MIX to simplify setup and standardization.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePredefined standard curve:\u003c\/strong\u003e Provided Vero DNA Control and recommended serial dilutions (ST1–ST6) for absolute quantitation and standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example instrument settings provided for common real-time PCR systems; parameters can be adjusted per instrument\/software.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControl strategy and QC guidance:\u003c\/strong\u003e Includes instructions for NTC, NCS, and Extraction Reference Control (ERC) with recovery acceptance criteria (50%–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing during biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in Vero cell lines\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 µL × 2 tubes); protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX (NNC016)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and maintain a DNA-free workspace (UV decontamination and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light during storage and handling.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run at least five concentrations on the standard curve; include NTC, NCS and ERC controls as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; ERC recovery should be between 50% and 150%; NCS Ct must be greater than mean Ct of lowest standard or less than validated LOQ if LOQ \u0026lt; lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Configure instrument-specific analysis parameters according to your instrument\/software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the kit sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended to quantify residual Vero DNA down to the femtogram (fg) level. The validated limit of quantitation (LOQ) should be determined by laboratory validation; if LOQ is below the lowest standard, ensure NCS Ct is less than the LOQ concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents sufficient for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended storage conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C. The components are stable for up to 24 months; check individual tube labels for expiration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which qPCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems. The user guide provides example settings for SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and LineGene 9600. For other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187363700917,"sku":"SK030204V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Quantitation_Kit.png?v=1742278593"},{"product_id":"residual-sv40-lta-e1a-dna-quantitation-kit-2g","title":"Residual SV40 LTA\/E1A DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n 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margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual SV40LTA \u0026amp; E1A DNA Quantitation Kit (2G) is a duplex real-time PCR (qPCR) kit designed to quantify residual SV40 large T antigen (LTA) and E1A host cell DNA (e.g., from HEK293T). The assay is duplex (simultaneous detection of SV40LTA and E1A) with an internal positive control (IPC) and achieves sensitivity to the level of 10^1 copies\/μL. The kit includes linear and non-linear DNA controls for standard curve generation and is compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96). Reagents are provided for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual SV40LTA and E1A host cell DNA in biological samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real-time PCR (qPCR) with target-specific primer\/probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10^1 copies\/μL (stated detection level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 4.67 × 10^6 to 4.67 × 10^1 copies\/μL (ST1–ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (non-linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 2.80 × 10^6 to 2.80 × 10^2 copies\/μL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl concentrations (as supplied)\u003c\/td\u003e\r\n \u003ctd\u003eLinear DNA Control: SV40LTA 4.67 × 10^9 copies\/μL; E1A 4.97 × 10^9 copies\/μL. Non-linear DNA Control: SV40LTA 2.80 × 10^9 copies\/μL; E1A 2.98 × 10^9 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation using standard curve (user-prepared serial dilutions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup \/ volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL sample or standard). qPCR MIX per reaction: qPCR Reaction Buffer 15.9 μL, Primer \u0026amp; Probe MIX 2.8 μL, IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15; Annealing\/extension 60 °C 1:00; 40 cycles. Fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other real-time PCR instruments supported with appropriate settings)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set Standard Curve (Absolute Quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within ±1.0 of the NCS Ct(IPC); significant increase indicates potential inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance thresholds\u003c\/td\u003e\r\n \u003ctd\u003eSV40LTA detection value of NTC should be ≤ 14.32 copies\/μL; E1A detection value of NTC should be ≤ 17.79 copies\/μL (or use user-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR:\u003c\/strong\u003e Simultaneous quantitation of SV40LTA and E1A targets in the same reaction using target-specific probes and an IPC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects down to 10^1 copies\/μL enabling detection of low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use controls and mixes:\u003c\/strong\u003e Includes linear and non-linear DNA controls, qPCR reaction buffer, primer\/probe mix, and IPC mix to support standard curve generation and assay QC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Validated on common qPCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96) and adaptable to other real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-defined analysis settings:\u003c\/strong\u003e Recommended thermal cycling and analysis parameters (e.g., threshold = 0.02, manual Ct) provided to standardize quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual SV40LTA and E1A host cell DNA in cell substrates (e.g., HEK293T)\u003c\/li\u003e\r\n \u003cli\u003eResidual host cell DNA monitoring for upstream\/downstream bioprocessing and product release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and standard curve generation for absolute quantitation of SV40-derived sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A linear DNA Control (NNA019) — lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A non-linear DNA Control (NNA020) — 50 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A Primer \u0026amp; Probe MIX (NNC030) — 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) — 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after opening per protocol)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect surfaces with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Reconstitute lyophilized control carefully: add 55 μL ddH2O, mix and let stand 10 minutes; perform serial dilutions as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer \u0026amp; Probe MIX, IPC MIX) from light and store at –20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five concentration points for the standard curve; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• DDB: store remaining unused DDB at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant shifts indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: SV40LTA ≤ 14.32 copies\/μL and E1A ≤ 17.79 copies\/μL (or use user-defined validation criteria).\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters per instrument and software version; recommended settings include manual Ct and threshold = 0.02.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit reports sensitivity to the level of 10^1 copies\/μL. Actual LOD\/LOQ should be confirmed during your laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500 (SDS v1.4 example) and Bio-Rad CFX96. Other instruments may be used with appropriate program and analysis adjustments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the qPCR thermal profile and analysis?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended program: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 (read fluorescence during annealing\/extension). Analysis: Manual Ct with threshold = 0.02 and Automatic Baseline; use Absolute Quantitation (standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I include and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude NTC, NCS, and at least five standard curve points (ST1–ST5; ST6 optional for linear control). IPC mean Ct should be within ±1.0 of NCS. NTC detection values should be ≤ 14.32 copies\/μL for SV40LTA and ≤ 17.79 copies\/μL for E1A, or use your validated criteria.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45191440335029,"sku":"1403443","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_SV40_LTA_E1A_DNA_Quantitation_Kit_2G.webp?v=1742280022"},{"product_id":"residual-pichia-pastoris-dna-quantitation-kit-shentek™","title":"Residual Pichia pastoris DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is a fluorescence-based quantitative PCR (FAM) assay designed to quantify residual Pichia pastoris host-cell DNA in biopharmaceutical materials across process stages (from in-process samples to final products). The kit includes a Pichia Pastoris DNA Control as a reference standard and provides rapid, specific quantitation with reliable sensitivity at the fg level. The kit workflow supports DNA sample dilution\/standard curve preparation, qPCR reaction mix setup and absolute quantitation using a standard curve. Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, StepOne Plus). For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Pichia pastoris DNA in biopharmaceutical samples (in-process and final products).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported quantitative range (from supplied standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 – 300 pg\/µL (equivalent to ~30 fg – 300 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReliable quantitation reported at the fg level; lowest supplied standard 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (qPCR mix 20 µL + sample 10 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003ePichia Pastoris qPCR Reaction Buffer 17 µL + Pichia Pastoris Primer\u0026amp;Probe MIX 3 µL -\u0026gt; 20 µL qPCR mix\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eProbe-based qPCR with FAM reporter dye (no quencher specified in kit instructions); use ROX as passive reference dye where applicable\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, CFX96, StepOne Plus, Linegene 9600plus, SHENTEK-96S and similar real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended. NTC Ct should be ≥ 35 cycles (or per internal validation). NCS Ct should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpike recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSpiked ERC recoveries required to be within 50% - 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ storage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stability up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003eExample workflow uses 100 µL test sample for preparation and 10 µL purified sample per qPCR reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Provides reliable quantitation at the fg level (lowest supplied standard 0.03 pg\/µL = 30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProbe-based specific detection:\u003c\/strong\u003e FAM-labeled probe assay offers high specificity for Pichia pastoris DNA fragments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard control:\u003c\/strong\u003e Includes Pichia Pastoris DNA Control for absolute quantitation and serial dilution standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated mix:\u003c\/strong\u003e Supplied reaction buffer and primer\/probe mix simplify qPCR setup and reduce preparation time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated with common real-time PCR platforms (ABI 7500, CFX96, StepOne Plus, Linegene, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow-ready for QC:\u003c\/strong\u003e Designed for in-process and final product host-cell DNA monitoring with recommended plate layouts and analysis settings.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Pichia pastoris host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and final product quality control for recombinant proteins produced in Pichia pastoris\u003c\/li\u003e\r\n \u003cli\u003eRoutine host-cell DNA monitoring during downstream purification and formulation\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike-recovery experiments for DNA residual assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris DNA Control (Part No. NNA007) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris qPCR Reaction Buffer (Part No. NNB005) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris Primer \u0026amp; Probe MIX (Part No. NNC041) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: thaw\/mix at 2–8 °C for use; unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-irradiate tabletop, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; handle primer\/probe and buffers on ice where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice and vortex to mix before use; spin briefly in microcentrifuge.\u003c\/p\u003e\r\n \u003cp\u003e• Check kit expiration date and storage conditions on labels prior to use.\u003c\/p\u003e\r\n \u003cp\u003e• Maintain recommended plate layout, replicate strategy and include NTC, NCS and ERC controls.\u003c\/p\u003e\r\n \u003cp\u003e• Spike recovery acceptance criteria: 50%–150%.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 cycles (or defined by internal validation).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit supports reliable quantitation at the fg level. The supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). Actual LOD in a given matrix should be confirmed by in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the Pichia Pastoris DNA Control and DNA Dilution Buffer (DDB) on ice. Prepare ST0 at 3000 pg\/µL then perform serial 1:10 dilutions to produce ST1–ST5: 300, 30, 3, 0.3 and 0.03 pg\/µL. Include at least five concentrations for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 95 °C for 10:00 (activation, 1 cycle), then 40 cycles of 95 °C for 00:15 (denaturation) and 60 °C for 01:00 (annealing\/extension, fluorescence read). Adjust settings if required by your instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should reagents be stored after thawing?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C per label. DNA Dilution Buffer (DDB) may be kept at 2–8 °C for short-term use; follow the instructions regarding storage of unused DDB and inspect for cloudiness\/precipitate before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171125813429,"sku":"SK030205P100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Pichia_Pastoris_DNA_Quantitation_Kit.jpg?v=1742277610"},{"product_id":"residual-hansenula-polymorpha-dna-quantitation-kit-shentek®","title":"Residual Hansenula polymorpha DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hansenula polymorpha DNA Quantitation Kit is designed to quantitatively detect and measure residual Hansenula polymorpha host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses fluorescent quantitative PCR (real-time qPCR) with a specific primer\/probe mix to provide rapid, specific and reliable quantitation at the femtogram (fg) level. The kit is supplied with DNA control material and reagents for sample dilution and qPCR reaction setup. For DNA extraction\/sample preparation procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Labeling\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ Yield\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (implied)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL to 300 pg\/µL (30 fg\/µL to 300 pg\/µL) — demonstrates femtogram-level detection sensitivity\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denature 95 °C 0:15 (40 cycles); Anneal\/Extend 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL (17 µL qPCR Reaction Buffer + 3 µL Primer\u0026amp;Probe MIX); Final reaction: 30 µL (add 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02; Automatic baseline; NTC Ct ≥ 35 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ recovery criteria\u003c\/td\u003e\r\n \u003ctd\u003eERC recovery acceptable range: 50%–150%; NCS Ct should be larger than mean Ct of lowest standard curve concentration (or less than LOQ if LOQ \u0026lt; lowest standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C; kit components stable for up to 24 months (check expiration on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Mx3000P, StepOne Plus, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual Hansenula polymorpha DNA at femtogram-level sensitivity (standard curve includes down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFluorescent real-time qPCR:\u003c\/strong\u003e Uses a validated primer \u0026amp; probe mix with FAM reporter for specific and rapid quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for quantitation:\u003c\/strong\u003e Includes DNA control, qPCR reaction buffer, primer \u0026amp; probe mix and DNA dilution buffer — sufficient reagents for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Protocols and settings provided for commonly used real-time PCR platforms; assay template provided for absolute quantitation (standard curve).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC and controls included:\u003c\/strong\u003e Guidance and materials for NTC, NCS and ERC controls; recommended acceptance criteria (NTC Ct ≥35; ERC recovery 50–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha DNA in final drug products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC testing for host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eResearch applications involving detection of Hansenula polymorpha DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha DNA Control (NNA008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha qPCR Reaction Buffer (NNB007)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 μL × 2 tubes) — protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha Primer \u0026amp; Probe MIX (NNC040)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate working surfaces, pipettes and tubes (UV irradiation 30 minutes recommended) and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C; check expiration dates.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer is cloudy, heat to 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (NTC, NCS, ERC) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Recommended qPCR analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35 cycles or undetermined; ERC recovery 50%–150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level detection; the provided standard curve covers 0.03 pg\/µL (30 fg\/µL) to 300 pg\/µL. Users should validate LOD\/LOQ in their own laboratory and instrument setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR program and settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 0:15 (denature) and 60 °C 1:00 (anneal\/extend, read fluorescence). Analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline. Adjust for your instrument if required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions of the provided DNA Control in DNA Dilution Buffer to generate at least five concentrations (example: 300, 30, 3, 0.3, 0.03 pg\/µL). Run each standard in triplicate for absolute quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are acceptable QC criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or Ct ≥ 35 cycles. NCS Ct should be larger than the mean Ct of the lowest standard or below the LOQ if LOQ is lower than the lowest standard. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171150553269,"sku":"SK030222HA100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hansenula_Polymorpha_DNA_Quantitation_Kit.jpg?v=1742277725"},{"product_id":"residual-saccharomyces-cerevisiae-dna-quantitation-kit-shentek®","title":"Residual Saccharomyces¬†cerevisiae DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Saccharomyces cerevisiae DNA Quantitation Kit is designed to quantify residual Saccharomyces cerevisiae DNA in biopharmaceutical materials across process stages from in-process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with analytical sensitivity at the femtogram (fg) level. For DNA extraction guidance, refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit supplies reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking standard range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standards prepared from DNA control: 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest standard (example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL (30 fg\/µL) included as lowest standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (document states fg-level sensitivity); practical detection guided by lowest standard and lab validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified; users should determine LOQ by laboratory validation. The lowest provided standard is 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR master mix per reaction 20 µL + 10 µL sample = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMaster mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eSaccharomyces cerevisiae qPCR Reaction Buffer 17 µL + Primer \u0026amp; Probe MIX 3 µL (total 20 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C 10:00 (1 cycle); Denature: 95 °C 00:15 (40 cycles); Anneal\/Extend: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dye \/ probe\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; no quencher specified in instructions (primer \u0026amp; probe mix provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold \/ analysis settings\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended Manual Ct threshold = 0.02; baseline = Automatic. NTC Ct should be ≥ 35.00 cycles or undetermined (or laboratory-specific criteria).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIncludes DNA control for standard curve, Negative Control Sample (NCS), No Template Control (NTC) and Extraction Reference Control (ERC). ERC recovery acceptance: 50%–150%. Ct(NCS) should be larger than mean Ct of lowest standard (or below LOQ if LOQ proven lower).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, samples and controls\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months when stored appropriately (see component-level storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended consumables\u003c\/td\u003e\r\n \u003ctd\u003eDNase-free low-retention tubes and tips; 96-well qPCR plates with sealing film or 8-strip PCR tubes with caps\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to detect Saccharomyces cerevisiae DNA down to the femtogram (fg) level; includes low-concentration standards (down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and reliable:\u003c\/strong\u003e Contains optimized primer \u0026amp; probe mix and qPCR reaction buffer for specific amplification and reproducible quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Supplies DNA control, qPCR reaction buffer and primer \u0026amp; probe mix to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated for use on multiple real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit components are stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eHost cell DNA residue testing during upstream\/downstream process development and release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike\/recovery experiments using ERC samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae DNA Control (Part No. NNA009) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae qPCR Reaction Buffer (Part No. NNB006) — 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae Primer \u0026amp; Probe MIX (Part No. NNC039) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and change tips frequently.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex to mix and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial dilution as instructed; include at least five concentrations for the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Triplicate measurements are recommended for standards, samples and controls.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35 or be undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery should be between 50% and 150%; verify extraction efficiency and assay performance.\u003c\/p\u003e\r\n \u003cp\u003e• Determine LOQ and assay validation parameters within your laboratory according to regulatory guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Agilent Mx3000PTM and StepOne Plus. If using other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the lowest standard concentration provided and does it represent the kit LOQ?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest provided standard is 0.03 pg\/µL (30 fg\/µL). The kit states fg-level sensitivity, but you should determine the limit of quantification (LOQ) for your matrix by laboratory validation; the provided lowest standard can be used as a reference point.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I handle and store unused DNA Dilution Buffer (DDB)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore remaining unused DDB at 2–8 °C. If the solution becomes cloudy or precipitated, warm to 37 °C until clear before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be ≥ 35 cycles or undetermined. Ct of NCS should be larger than the mean Ct of the lowest standard (or below LOQ if the LOQ is proven lower than the lowest standard). ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171176636597,"sku":"1101103","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Saccharomyces_Cerevisiae_DNA_Quantitation_Kit.jpg?v=1742277789"},{"product_id":"residual-cho-dna-quantitation-kit-shentek®","title":"Residual CHO DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit is designed for quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (absolute quantitation with a standard curve) to perform rapid and specific quantitation of residual DNA at the femtogram level. For nucleic acid extraction and sample preparation guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100). The kit is supplied as reagents for 100 reactions and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical products (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by quantitative PCR (standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard concentrations prepared from CHO DNA Control: ST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL; ST6 = 0.003 pg\/μL. (Implied dynamic range: 300 to 0.003 pg\/μL using provided standards.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eStandards include down to 0.003 pg\/μL (ST6). No explicit LOQ\/LOD numeric claim provided; users should validate limit of quantitation (QL) in their own laboratory.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or standard).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample (or 10 μL standard\/controls as described).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix provided\u003c\/td\u003e\r\n \u003ctd\u003eReady-to-use CHO qPCR MIX supplied (mix thoroughly before use); protect from light.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C for 10:00; 40 cycles of Denaturation 95 °C for 0:15 and Annealing\/Extension 60 °C for 1:00 (fluorescence read at 60 °C).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling count\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — ERC recovery\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecovery of Extraction Reference Control (ERC) samples should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — NTC \/ NCS\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct should be Undetermined or not less than 35.00 (or laboratory-specific criteria). NCS Ct should be larger than the mean Ct of the lowest standard; if proven QL is lower than minimum standard concentration, NCS detected value should be less than QL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 Plus, Mx3000P, qTOWER 3 G, StepOnePlus (consult instrument documentation for setup).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components may be stored under recommended conditions for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended analysis settings (example ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set task types for standard, unknown, NTC as described.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of detecting residual CHO DNA down to femtogram-level concentrations (standards provided to 0.003 pg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Provided CHO qPCR MIX formulated for absolute quantitation—minimizes preparation steps and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard curve control:\u003c\/strong\u003e Includes CHO DNA Control and DNA Dilution Buffer to prepare a 6-point standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR platforms; instrument-specific setup instructions included.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow guidance:\u003c\/strong\u003e User guide includes serial dilution protocol, plate layout examples, thermal cycling program, and acceptance criteria (ERC recovery, NTC\/NCS rules).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics production (host cell DNA monitoring)\u003c\/li\u003e\r\n \u003cli\u003eProcess development and purification monitoring for cell-derived products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing in research settings\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (Part No. NNA001)\u003c\/span\u003e\u003cstrong\u003e50 μL × 1 tube; store at –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO qPCR MIX (Part No. NNC001)\u003c\/span\u003e\u003cstrong\u003e1 mL × 2 tubes; store at –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL × 3 tubes; store at –20 °C (remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (absolute quantitation, standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eye protection, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and centrifuge briefly; mix CHO qPCR MIX thoroughly and protect from light.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific setup and analysis parameters; example settings are provided for ABI 7500 SDS v1.4 but may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at recommended temperatures; per USP \u0026lt;659\u0026gt; items with –20 °C recommendation should be stored within ±10 °C (–30 °C to –10 °C).\u003c\/p\u003e\r\n \u003cp\u003e• Validate limit of quantitation (QL) and other performance metrics in your laboratory prior to routine testing.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure NTC (no template control) is undetermined or Ct ≥ 35 and NCS meets acceptance criteria before reporting sample results.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity of the kit and how low can it quantify CHO DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve includes concentrations down to 0.003 pg\/μL (ST6). The kit enables femtogram-level detection, but users should perform laboratory validation to determine the assay’s limit of quantitation (QL) under their specific conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL CHO qPCR MIX + 10 μL sample or standard). Use 10 μL of purified test sample per reaction as described in the user guide.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should reagents be stored and what is kit stability?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore CHO DNA Control, CHO qPCR MIX and DNA Dilution Buffer at –20 °C (protect qPCR mix from light). Kit components are stable for up to 24 months; check expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC shows amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC (no template control) is amplified (Ct \u0026lt; 35 or determined), suspect contamination. Decontaminate workspace and consumables (UV, 75% ethanol), re-prepare master mixes and controls, and repeat the assay. Verify pipetting technique and use fresh aliquots of reagents.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171252527285,"sku":"SK030201C100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit.jpg?v=1742278019"},{"product_id":"residual-ns0-sp2-0-dna-quantitation-kit-shentek®","title":"Residual NS0 \u0026 SP2\/0 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual NS0\u0026amp;SP2\/0 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical matrices (in-process samples to final products). The kit provides NS0\u0026amp;SP2\/0 DNA Control as a reference standard and contains qPCR Reaction Buffer, NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX, and DNA Dilution Buffer. The assay is designed for sensitive, specific and rapid quantitation at the femtogram (fg) level. Reagents in this kit are sufficient for 100 reactions. For DNA extraction procedures refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR using a supplied standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; lowest kit standard = 0.003 pg\/µL (3 fg\/µL). Laboratory validation required to establish LOD\/LOQ.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL (ST0 stock: 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX 3 µL = 20 µL per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (data acquisition during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, Bio-Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥ 35 cycles or undetermined (or per lab validation); Ct of NCS should be \u0026gt; mean Ct of lowest standard or below validated LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable for up to 24 months when stored as instructed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReference \/ related product\u003c\/td\u003e\r\n \u003ctd\u003eFor extraction information see SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at femtogram (fg) level; lowest supplied standard 0.003 pg\/µL\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Supplies DNA control and primer\/probe mix for construction of standard curve and absolute quantitation\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX minimize assay setup variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use on multiple common real-time PCR platforms\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuality controls included:\u003c\/strong\u003e Includes NS0\u0026amp;SP2\/0 DNA Control for standard curve and guidance for NTC, NCS and ERC controls\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual NS0 host cell DNA in biopharmaceutical process intermediates and final drug substance\/formulation\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual SP2\/0 host cell DNA in biopharmaceutical samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA testing in QC and R\u0026amp;D (Research Use Only)\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 DNA Control (Part No. NNA021)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 µL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX (Part No. NNC029)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes (remaining unused DDB store 2–8 °C; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory clothing and gloves\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and dedicated workspace\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate workspace, pipettes and tubes with UV for 30 minutes and 75% ethanol before setup\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly prior to use\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates\/strips and spin briefly before running qPCR\u003c\/p\u003e\r\n \u003cp\u003e• Establish laboratory-specific LOQ\/LOD by validation; NTC Ct acceptance: ≥ 35 cycles or undetermined\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance: 50%–150%\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration standard provided in the kit and what sensitivity can I expect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest supplied standard (ST6) is 0.003 pg\/µL (3 fg\/µL). The kit is designed to enable quantitation at the femtogram level; however, actual limit of detection (LOD) and limit of quantitation (LOQ) should be established by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the NS0\u0026amp;SP2\/0 DNA Control and DNA Dilution Buffer at 2–8 °C. Dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to prepare ST1–ST6 (ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL). Include at least five concentrations on the standard curve for quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What qPCR cycling conditions and reaction setup should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction volume (20 µL qPCR mix + 10 µL sample). Cycling: Activation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 0:15 (40 cycles); Annealing\/Extension 60 °C for 1:00 with fluorescence read during this step. Prepare qPCR mix per reaction: 17 µL qPCR Reaction Buffer + 3 µL Primer \u0026amp; Probe MIX.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I interpret controls and QC criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35 (or per lab validation). NCS Ct should be larger than mean Ct of lowest standard in the curve or below validated LOQ. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171267436725,"sku":"SK030208N100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_NS0_SP2_0_DNA_Quantitation_Kit.jpg?v=1742278378"},{"product_id":"residual-vero-dna-154-quantitation-kit-shentek®","title":"Residual Vero DNA-154 Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA-154 Quantitation Kit is a research-use qPCR kit for quantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process to final products). The kit uses a fluorescent quantitative PCR (FAM reporter) assay with a pre-formulated primer \u0026amp; probe mix and Vero DNA Control standard to enable rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit supplies Vero DNA Control as a reference standard and DNA Dilution Buffer for standard curve preparation. For nucleic acid extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (TaqMan-style probe); absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (documented serial dilutions down to 0.0003 pg\/μL = 0.3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003ePrepared from Vero DNA Control: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction (20 μL qPCR MIX + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + Vero Primer \u0026amp; Probe MIX-154 3 μL = 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReporter \/ Reference dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR instrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus (and similar real-time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTriplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality criteria \/ acceptance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate should be 50%–150%; Ct of NTC should be ≥35 or undetermined; Ct of NCS should be larger than mean Ct of lowest standard (or below validated LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months when stored as instructed (-20 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating Vero host DNA down to the femtogram level using an optimized primer\/probe assay and standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes pre-formulated Vero Primer \u0026amp; Probe MIX-154 for consistent, specific detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSupplied reference standard:\u003c\/strong\u003e Vero DNA Control provided for preparation of an 8-point serial dilution standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with multiple qPCR platforms:\u003c\/strong\u003e Validated workflows and example settings included for several common real-time PCR instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eUser guidance for QC:\u003c\/strong\u003e Includes instructions for NTC, NCS, ERC controls and acceptance criteria (ERC recovery 50%–150%, NTC Ct ≥35).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of drug substance and drug product for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of downstream purification steps\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-154 (NNC018)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 μL × 1 tube; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance range: 50%–150%; ensure NTC Ct ≥35 or undetermined.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample). qPCR MIX per reaction is composed of 17 μL qPCR Reaction Buffer + 3 μL Vero Primer \u0026amp; Probe MIX-154.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve concentrations should I prepare?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the provided Vero DNA Control as follows: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL. Include at least five concentration points in the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be within 50%–150%. The Ct for NTC should be ≥35 or undetermined. The Ct for NCS should be greater than the mean Ct of the lowest standard (or below validated LOQ if LOQ is lower than the lowest standard).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus, and similar instruments. Adjust analysis settings to your instrument\/software as needed.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171280609461,"sku":"SK030227V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA-154_Quantitation_Kit.jpg?v=1742278786"},{"product_id":"residual-mdck-dna-quantitation-kit-shentek®","title":"Residual MDCK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Quantitation Kit is designed to quantitate residual MDCK host cell DNA in biopharmaceutical workflows, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) for rapid, specific and reliable absolute quantitation at the femtogram (fg) level. The kit includes a MDCK DNA control, primer \u0026amp; probe mix, reaction buffer and DNA dilution buffer. For nucleic acid extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical samples (in‑process and final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative PCR (qPCR), absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims detection at femtogram (fg) level; lowest provided standard concentration = 0.003 pg\/μL (3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Linear range (provided)\u003c\/td\u003e\r\n \u003ctd\u003eStandard concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03, 0.003 pg\/μL (ST0–ST6); at least five concentrations recommended for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Quantitation (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eLOQ not explicitly specified; protocol notes use of validated LOQ; if LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal qPCR reaction volume = 30 μL (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + MDCK Primer \u0026amp; Probe MIX 3 μL = 20 μL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real‑time PCR instruments (SHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; Negative Control Sample (NCS) Ct should be larger than mean Ct of lowest standard (or less than LOQ if validated LOQ is lower); ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as labeled Product No.: SK030209M100)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components storable under appropriate conditions up to 24 months; individual components stored at -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay readout \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eRecommended analysis: Manual Ct, threshold for MDCK‑DNA = 0.02, automatic baseline; set tasks (Standard, Unknown, NTC) and verify slope, intercept, R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual MDCK DNA down to the femtogram level (lowest provided standard 0.003 pg\/μL = 3 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Standard‑curve based absolute quantitation using supplied MDCK DNA control and serial dilutions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes validated MDCK Primer \u0026amp; Probe MIX to simplify assay setup and improve reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on multiple common real‑time PCR platforms (ABI 7500, CFX96, StepOne Plus, LineGene, Mx3000P, SHENTEK‑96S, etc.).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eKit format:\u003c\/strong\u003e Provides reagents for 100 reactions with pre‑measured components (control, buffer, primer\/probe mix and dilution buffer).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process development\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support for final biopharmaceutical products (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for MDCK host cell DNA detection\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) — 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX (NNC024) — 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or therapeutic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low‑retention consumables; decontaminate workspace and pipettes with UV (30 min) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly; store unused DNA Dilution Buffer at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light‑sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, ERC and standard curve in each run.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument\/software‑specific parameter settings; example settings provided for ABI 7500 with SDS v1.4.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest standard concentration provided and what is the claimed sensitivity?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided serial dilution scheme includes standards down to 0.003 pg\/μL (3 fg\/μL). The kit is intended to enable quantitation at the femtogram level.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 20 μL qPCR mix per reaction (17 μL qPCR Reaction Buffer + 3 μL Primer \u0026amp; Probe MIX) and add 10 μL sample\/standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample program: Activation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15 (40 cycles); Annealing\/extension 60 °C 1:00 with fluorescence read during this step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should be used for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than the mean Ct of the lowest standard (or below validated LOQ if applicable); ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171293880501,"sku":"SK030209M100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Quantitation_Kit.jpg?v=1742279376"},{"product_id":"residual-sf9-acnpv-dna-quantitation-kit-shentek®","title":"Residual Sf9 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9\u0026amp;AcNPV DNA Quantitation Kit is designed for quantitative measurement of residual Sf9 and AcNPV host cell DNA in biopharmaceutical materials at different processing stages (in-process to final products). The kit uses a multiplex quantitative PCR (qPCR) assay with internal positive control (IPC) to provide rapid, specific and reliable quantitation down to femtogram-level sensitivity. Supplied reagents include DNA control standards for Sf9 and AcNPV, primer\/probe mixes, IPC mix, qPCR reaction buffer and DNA dilution buffer. The kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A). Refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction guidance.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (Sf9)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e300 pg\/µL to 0.003 pg\/µL (standard curve points: 300, 30, 3, 0.3, 0.03, 0.003 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (AcNPV)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5.14×10^6 to 5.14×10^1 copies\/µL (equivalent mass: 20 to 0.0002 pg\/µL; standard curve points provided for both copies\/µL and pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity down to femtogram (fg) level implied by standard curve lowest points (Sf9: 0.003 pg\/µL = 3 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReaction total volume 30 µL per well (20 µL qPCR MIX + 10 µL sample).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL (total 20 µL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C for 10:00 (1 cycle); Denaturation: 95 °C for 0:15; Annealing\/Extension: 60 °C for 1:00 (read fluorescence). 40 cycles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument channels \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eSf9-DNA: FAM; AcNPV-DNA: CY5; IPC: VIC; Passive reference dye: ROX (ABI 7500 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ quantitation settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold 0.02 for Sf9-DNA, AcNPV-DNA and IPC; use Absolute Quantitation (Standard Curve) analysis.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC: Ct(sample) should be within ±1.0 Ct of Ct(NCS) — large deviation indicates inhibition. NTC: Ct ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as shipped).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see component-specific storage: most components -20 °C; protect qPCR Reaction Buffer from light).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ baseline guidance\u003c\/td\u003e\r\n \u003ctd\u003eThreshold set to 0.02; Automatic baseline recommended when using provided settings.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR:\u003c\/strong\u003e Simultaneous detection and quantitation of Sf9-DNA, AcNPV-DNA and IPC in one reaction using distinct fluorescent channels (FAM, CY5, VIC).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay standard curve includes femtogram-level concentrations (Sf9 down to 0.003 pg\/µL) enabling detection at fg scale.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC provided to monitor PCR inhibition and overall reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix, IPC mix and reaction buffer simplify setup and reduce pipetting errors.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated examples and guidance provided for multiple real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, CFX96, FQD-96A).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStandardized quantitation:\u003c\/strong\u003e Supplied Sf9 \u0026amp; AcNPV DNA controls with serial dilution scheme for generating absolute standard curves (copies\/µL and pg\/µL).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host cell DNA in biopharmaceutical process and final drug substance\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Autographa californica multiple nucleopolyhedrovirus (AcNPV) DNA in bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control and release testing for products produced in Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV DNA Control (NNA051), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX (NNC059), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC067), 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused remaining DDB may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free area; use DNase-free, low-retention consumables and tips.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate work surfaces and equipment for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial 10-fold dilutions in DDB as instructed; at least five standard concentrations recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates well, mix and spin down prior to starting qPCR to avoid evaporation and inconsistent Ct values.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC: sample Ct-IPC should be within ±1.0 Ct of NCS; significant increase indicates sample inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35.00 or be undetermined; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents for 100 reactions (stated on the product cover).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and thermal program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 30 µL reactions (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 with fluorescence read during the 60 °C step. Use manual Ct threshold 0.02 as guidance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than Ct-IPC of NCS (beyond ±1.0 Ct), consider sample dilution, re-extraction, or purification to remove inhibitors. Use recovery of spiked reference standards to assess sample processing.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the assay dynamic ranges and units?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSf9 standard curve: 300 to 0.003 pg\/µL. AcNPV standard curve: 5.14×10^6 to 5.14×10^1 copies\/µL (equivalent to 20 to 0.0002 pg\/µL). At least five standard concentrations should be used for curve generation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171362431157,"sku":"1101101","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_AcNPV_DNA_Quantitation_Kit.jpg?v=1742279477"},{"product_id":"residual-cv-1-dna-quantitation-kit-shentek-n","title":"Residual CV-1 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CV-1 DNA Quantitation Kit is designed to quantitate residual CV-1 host cell DNA across stages of biopharmaceutical production, from in-process samples to final products. The kit uses quantitative PCR (qPCR) to deliver rapid, specific and reliable quantitation at the femtogram (fg) level and includes a CV-1 DNA Control reference standard. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST0: 3000 pg\/µL; ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest included standard (indicative sensitivity)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.003 pg\/µL (3 fg\/µL) — kit supports femtogram-level quantitation; validated LOQ must be determined by laboratory validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (20 µL qPCR mix + 10 µL sample\/diluent)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer: 17 µL; CV-1 Primer \u0026amp; Probe MIX: 3 µL; Total qPCR MIX per reaction: 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C, 10:00 (1 cycle); Denaturation: 95 °C, 00:15 (40 cycles); Annealing\/Extension: 60 °C, 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection settings\u003c\/td\u003e\r\n \u003ctd\u003eRecommended reporter dye: FAM; passive reference: ROX (ABI7500 example). Manual Ct threshold example: 0.02 (Automatic baseline recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 cycles or undetermined (or per laboratory validation); NCS Ct should be larger than mean Ct of lowest standard (or below LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months at recommended storage conditions (component-specific temperatures listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims femtogram-level detection; lowest provided standard 0.003 pg\/µL (3 fg\/µL) allowing very low-level residual DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Absolute quantitation using a supplied CV-1 DNA Control standard curve (six dilution points recommended).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX to streamline assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComprehensive workflow support:\u003c\/strong\u003e Includes guidance for standard curve preparation, plate layout recommendations, qPCR program settings and result analysis instructions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in final drug substance\/drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in CV-1 cells\u003c\/li\u003e\r\n \u003cli\u003eProcess development and optimization where host-cell DNA monitoring is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 DNA Control (NNA026)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 Primer \u0026amp; Probe MIX (NNC097)\u003c\/span\u003e\u003cstrong\u003eStore as labeled (provided 300 μL × 1 tube); follow kit storage instructions\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C during use); 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation\u003c\/span\u003e\u003cstrong\u003eUser Guide and instructions included\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for human diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and pipettes with UV (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice (2–8 °C), vortex and spin briefly before use; protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh using serial dilutions; include at least five concentrations for curve fitting.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates with appropriate film, mix and spin briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC Ct ≥35 cycles or undetermined (or per lab validation); NCS Ct must be \u0026gt; mean Ct of lowest standard or below validated LOQ.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific program and detector settings must be configured per instrument\/software documentation (ABI7500 example provided).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity or lowest detectable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides a lowest standard of 0.003 pg\/µL (3 fg\/µL) and is designed to support femtogram-level quantitation. The validated limit of quantitation (LOQ) should be determined by laboratory validation and may be lower or higher depending on sample matrix and extraction recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should reagents be stored and handled?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures (major components at -20 °C). Thaw reagents on ice (2–8 °C), vortex and spin briefly before use. Protect light-sensitive components (e.g., qPCR Reaction Buffer) from light. Follow MSDS guidance and use DNase-free consumables.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should have Ct ≥35 cycles or be undetermined (or per laboratory validation). The NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve or below the validated LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are supported and what detector settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). In the ABI7500 example, create a FAM detector (reporter FAM), set ROX as passive reference, use Manual Ct threshold 0.02 and Automatic baseline; thermal cycling: 95 °C activation 10:00; 40 cycles of 95 °C 00:15 and 60 °C 1:00 (read fluorescence during anneal\/extension). Adjust settings per instrument and software version.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187376840885,"sku":"1101105","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CV-1_DNA_Quantitation_Kit.webp?v=1742279215"},{"product_id":"residual-hi5-acnpv-dna-quantitation-kit-shentek®","title":"Residual Hi5 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n 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8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hi5\u0026amp;AcNPV DNA Quantitation Kit is designed to quantify residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses a multiplex quantitative PCR (qPCR) assay with target-specific primer\/probe mixes for Hi5 DNA and AcNPV DNA plus an Internal Positive Control (IPC) to monitor reaction performance and potential inhibition. The assay supports absolute quantitation using a supplied lyophilized DNA control and DDB-based serial dilutions. The kit provides reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples across process stages\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMultiplex quantitative PCR (qPCR) with three detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 μL total per well (20 μL qPCR mix + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; Hi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eHi5 DNA standard curve (mass)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points: 30 pg\/μL (ST1), 3 pg\/μL (ST2), 0.3 pg\/μL (ST3), 0.03 pg\/μL (ST4), 0.003 pg\/μL (ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcNPV DNA standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points (copies\/μL and pg\/μL): ST1 5.14×10^5 copies\/μL (2 pg\/μL), ST2 5.14×10^4 (0.2 pg\/μL), ST3 5.14×10^3 (0.02 pg\/μL), ST4 5.14×10^2 (0.002 pg\/μL), ST5 5.14×10^1 (0.0002 pg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity claim\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level is supported by the provided standard curve down to 0.003 pg\/μL for Hi5 and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC Ct of sample vs NCS should be within ±1.0 Ct; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger (i.e., later) than mean Ct of lowest standard; NCS should show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample (per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable up to 24 months when stored as directed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided quantity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions (kit contains reagents sized as listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated LOQ is referenced in method validation; the user should determine LOQ during method validation. If LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR format:\u003c\/strong\u003e Simultaneous detection of Hi5 DNA, AcNPV DNA and IPC in a single reaction (FAM, CY5, VIC channels)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Supports quantitation down to femtogram-level concentrations using supplied standards (mass and copy-number options for AcNPV)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify inhibited samples\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer\u0026amp;Probe MIX and IPC MIX reduce assay setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hi5 host-cell DNA in biopharmaceutical process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual AcNPV DNA (baculovirus) in upstream and downstream process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support and method development for biologics produced in Hi5\/AcNPV systems\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV DNA Control (lyophilized powder) ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C (after opening\/for use, store remaining DDB at 2–8 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, mask, protective eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout recommendations and include at least five standard curve points, NTC and NCS in each run.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: Ct(sample IPC) must be within ±1.0 Ct of Ct(NCS) — higher IPC Ct indicates potential inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: Ct ≥ 35 or undetermined; NTC should not show target amplification.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Validated LOQ must be established during method validation; if LOQ is below the lowest standard, ensure NCS value is below LOQ.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration for Hi5 and AcNPV using this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve supports quantitation down to 0.003 pg\/μL for Hi5 DNA and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV DNA. Users should perform method validation to determine assay LOD\/LOQ for their matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample should be added per reaction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 10 μL of purified test sample per 30 μL reaction (20 μL qPCR mix + 10 μL sample).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the instrument settings for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal profile: Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 01:00 with fluorescence read at 60 °C. Set reaction volume to 30 μL. Configure detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC Ct is significantly higher than NCS IPC Ct (outside ±1.0 Ct), suspect inhibition. Consider diluting the sample, re-extracting with improved purification, or including spike-recovery controls to assess sample recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173391065269,"sku":"1101102","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hi5_AcNPV_DNA_Quantitation_Kit.png?v=1742279579"},{"product_id":"residual-human-dna-quantitation-kit-shentek®","title":"Residual Human DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Human DNA Quantitation Kit (2G) is a duplex quantitative PCR (qPCR) kit for quantitation of residual human host-cell DNA in biopharmaceutical samples from in-process to final products. The assay achieves femtogram-level sensitivity and includes an Internal Positive Control (IPC) in the Human Primer \u0026amp; Probe MIX to monitor PCR performance. The kit contains a Human DNA Control for standard curve generation and ready-to-use qPCR Master MIX and Primer\u0026amp;Probe MIX. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual human host-cell DNA in biopharmaceutical samples (in-process to final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex quantitative PCR (qPCR) with Human-DNA detector (FAM) and IPC (VIC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level; NTC detection value acceptance ≤ 6.00 fg\/μL (user may set criteria by method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical linear range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e300 pg\/μL to 0.03 pg\/μL (example standard series provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis thresholds (example)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-DNA = 0.05; IPC = 0.1; Automatic baseline\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate 50%–150%; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification; NTC detection ≤ 6.00 fg\/μL and IPC amplification normal\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePack size \/ yield\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions (product no. 1101108)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored at appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation at femtogram (fg) level\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes Internal Positive Control in the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and Primer\u0026amp;Probe MIX reduce setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR systems (SHENTEK-96S, ABI 7500, CFX96, LightCycler 480)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow convenience:\u003c\/strong\u003e Includes Human DNA Control and DNA Dilution Buffer with recommended serial dilution scheme for standard curve generation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human host-cell DNA in biopharmaceutical products (in-process and final product)\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring and release testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eComparative testing of extraction methods (use with Extraction Reference Control, ERC)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer \u0026amp; Probe MIX (includes IPC) (NNC130) 500 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReagents for 100 reactions (kit pack information)\u003c\/span\u003e\u003cstrong\u003e-20 °C (components stable up to 24 months)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination. UV decontaminate work surfaces and pipettes prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Master MIX from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, and ERC. Run standards (at least five concentrations) to build a standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC ≤ 6.00 fg\/μL (or criteria defined during method validation); NCS Ct larger than mean Ct of lowest standard and shows IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR analysis parameters (e.g., thresholds, baseline) should be configured for the specific instrument and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level quantitation. Example standard points include down to 0.03 pg\/μL (30 fg\/μL). The kit documentation states NTC detection acceptance ≤ 6.00 fg\/μL; true LOD\/LOQ should be determined by the user's method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Roche LightCycler 480. It is compatible with other real-time PCR instruments that support dual-channel detection (FAM and VIC), but instrument-specific setup is required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How many reactions are included and how should reagents be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Store components at -20 °C and protect the qPCR Master MIX from light. Components are stable up to 24 months—confirm expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I run and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRun a standard curve (minimum five points), Non-Template Control (NTC), Negative Control Sample (NCS), and Extraction Reference Control (ERC). ERC recovery should be between 50% and 150%. NTC detection should be ≤ 6.00 fg\/μL (or per user validation). NCS Ct should be larger than the mean Ct of the lowest standard and show IPC amplification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173398765749,"sku":"1101108","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Quantitation_Kit.png?v=1742279705"},{"product_id":"residual-bhk-dna-quantitation-kit-shentek®","title":"Residual BHK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Quantitation Kit is designed to quantitate residual BHK host cell DNA in different stages of biopharmaceutical products, from in‑process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit includes a BHK DNA Control and reagents for preparing standard curves and performing absolute quantitation. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical samples (in‑process to final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by fluorescent qPCR (standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects at the femtogram (fg) level (laboratory validation recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e3000 pg\/µL, 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (ST0–ST5 as prepared per protocol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + BHK Primer\u0026amp;Probe MIX 3 µL → 20 µL qPCR mix; add 10 µL sample\/standard to reach 30 µL total\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: none (per setup instructions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard (or larger than LOQ Ct if LOQ is below lowest standard); ERC recovery rate should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; stable up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reaction count\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents supplied for 100 reactions (kit format)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating residual BHK DNA at the femtogram (fg) level when properly validated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes BHK DNA Control and standard curve workflow for absolute quantitation of target DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Contains qPCR Reaction Buffer and Primer \u0026amp; Probe MIX optimized for the assay to reduce setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated for use on SHENTEK‑96S, ABI 7500, Roche LightCycler 480 II and other real‑time PCR systems (instrument settings may vary).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Intended for testing residual host cell DNA across in‑process samples to final product stages and for assay validation workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing for residual DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003cli\u003eQuality control and stability studies involving host cell DNA measurements\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (Part No. NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX (Part No. NNC076) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low retention consumables and clean work area (UV irradiation and 75% ethanol decontamination recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly spin before use; protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration date on the label.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points for the standard curve and perform triplicates for samples and controls where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery rates should be between 50% and 150%; investigate deviations.\u003c\/p\u003e\r\n \u003cp\u003e• Set instrument analysis parameters (thresholds, baseline, detector settings) according to instrument\/software and laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to enable quantitation at the femtogram (fg) level. The practical limit of detection (LOD) and limit of quantification (LOQ) should be established by laboratory validation. The provided standard curve includes concentrations down to 0.03 pg\/µL (30 fg\/µL as prepared).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real‑time PCR instruments. The user guide provides example settings for SHENTEK‑96S, ABI 7500 (SDS v1.4), and Roche LightCycler 480 II. Adjust instrument and software parameters as needed for other platforms and validate performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the BHK DNA Control and DNA Dilution Buffer at 2–8 °C, dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentration points and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC or NCS show amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC shows amplification (Ct \u0026lt; 35), verify reagent and plate handling to identify contamination sources, replace consumables, re‑prepare reagents, decontaminate work surfaces and equipment (UV\/ethanol), and repeat the assay. If NCS Ct is not larger than the lowest standard Ct or LOQ Ct, review extraction and dilution procedures and revalidate the LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173411610805,"sku":"1101116","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Quantitation_Kit.png?v=1742279915"},{"product_id":"residual-e1a-dna-quantitation-kit-shentek®","title":"Residual E1A DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1A DNA Quantitation Kit is designed for the quantitation of residual E1A DNA derived from host cells (e.g., HEK293 and 293T) across different stages of biopharmaceutical manufacture, from in-process samples to final products. The kit uses fluorescent quantitative PCR (real-time qPCR) for rapid, specific and reliable absolute quantitation. An Internal Positive Control (IPC) is included to monitor PCR performance and potential inhibition. The kit provides both E1A linear DNA Control and E1A non-linear DNA Control to accommodate different user needs. Recommended sample extraction procedures are provided in the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e4.97 × 10^8 copies\/μL (stock) with serial 10-fold dilutions down to 4.97 × 10^1 copies\/μL (ST1–ST6 covering 4.97 × 10^6 to 4.97 × 10^1 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (non-linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e2.98 × 10^7 copies\/μL (stock) with serial 10-fold dilutions down to 2.98 × 10^2 copies\/μL (ST0–ST5 covering 2.98 × 10^6 to 2.98 × 10^2 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; E1A Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample or purified NCS per reaction; recommended starting material for extraction: 100 μL sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection dyes\u003c\/td\u003e\r\n \u003ctd\u003eE1A-DNA detector: CY5; IPC detector: VIC; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for both E1A-DNA and IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt value of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; significant shift indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ \/ LOD\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ) not specified in kit documentation; users should determine proven LOQ during method validation. If proven LOQ is lower than lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended replicates and standard points\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended; at least five concentration points recommended for standard curve (six when using linear DNA control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eValidated instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other compatible real-time PCR systems may be used)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and sensitive absolute quantitation:\u003c\/strong\u003e Fluorescent qPCR assay for quantitative detection of residual E1A DNA with serial standards to create an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify potential sample inhibition; IPC results used to assess recovery when spiked samples are tested.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTwo control formats provided:\u003c\/strong\u003e Both E1A linear DNA Control (lyophilized) and E1A non-linear DNA Control are supplied so users can choose according to validation needs.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX supplied in ready-to-use formats to simplify setup; protect light-sensitive reagents from light.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, ABI 7500, CFX96); thermal and detector settings provided for instrument configuration.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1A DNA from host cells (e.g., HEK293, 293T) in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing and final product testing for residual host-cell E1A DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and QC workflows requiring absolute quantitation of E1A sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A linear DNA Control (lyophilized powder × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A non-linear DNA Control (50 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A Primer \u0026amp; Probe MIX (300 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow laboratory safety procedures; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration dates. Do not use if reagents show precipitate or cloudiness (heat DDB at 37 °C to clarify if necessary).\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, standards and IPC. Use triplicates for samples and standards for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific settings should be adapted to the user's instrument and software version; follow provided detector\/dye assignments and thermal profile as guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitation of residual E1A DNA from host cell-derived samples (for example, HEK293 and 293T) and is applicable to samples collected during biopharmaceutical manufacturing (in-process and final product). Samples must be purified prior to qPCR according to the recommended extraction workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is the standard curve prepared and what dynamic range is covered?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are prepared by serial 10-fold dilutions of the provided DNA controls. The linear DNA control stock is 4.97 × 10^8 copies\/μL (diluted down to 4.97 × 10^1 copies\/μL across ST1–ST6); the non-linear control stock is 2.98 × 10^7 copies\/μL (diluted down to 2.98 × 10^2 copies\/μL across ST0–ST5). At least five standard points (six for linear control) are recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What quality criteria should I use to accept a run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35. IPC Ct for sample should be within ±1.0 Ct of IPC Ct for NCS; significant shifts indicate inhibition. Verify standard curve slope, intercept and R^2 before reporting sample quantities.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the limit of quantification (LOQ) provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eA numeric LOQ is not specified in the kit documentation. Users should determine the proven LOQ during method validation for their specific matrix and instrument. If proven LOQ is below the lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173426094261,"sku":"1101109","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1A_DNA_Quantitation_Kit.png?v=1742280136"},{"product_id":"residual-e1b-dna-quantitation-kit-shentek®","title":"Residual E1B DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1B DNA Quantitation Kit is designed for absolute quantitation of residual E1B DNA derived from host cells (e.g., HEK293 and derivative cell lines such as 293T and 293F) in various stages of biopharmaceutical manufacturing (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (qPCR) assay with an internal positive control (IPC) to monitor PCR performance and includes an E1B linear DNA Control for preparation of standard curves and for detection of linearized samples. The kit is supplied for 100 reactions and is intended for research use only. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by fluorescent qPCR (FAM-labeled target, VIC-labeled IPC, ROX passive reference)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eST1 to ST6: 4.34 × 10^6 to 4.34 × 10^1 copies\/μL (prepared from E1B linear DNA Control; original ST = 4.34 × 10^8 copies\/μL before serial dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL, E1B Primer\u0026amp;Probe MIX 2.8 μL, IPC MIX 1.3 μL (total qPCR MIX = 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00; Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument settings \/ analysis\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 for both E1B-DNA and IPC; Automatic baseline; Standard curve (absolute quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control acceptance\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; a significantly higher Ct(IPC) indicates potential PCR inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input for preparation\u003c\/td\u003e\r\n \u003ctd\u003e100 μL test sample (or 100 μL DDB for negative control sample preparation prior to extraction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under stated conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification \/ limit of detection\u003c\/td\u003e\r\n \u003ctd\u003eNot numerically specified in the manual. Users should establish LOD\/LOQ by validation; standard curve lowest point in user protocol is 4.34 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh specificity and sensitivity:\u003c\/strong\u003e Fluorescent qPCR assay with sequence-specific primers and probe for E1B enabling specific detection of residual E1B DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Included IPC MIX monitors PCR performance and helps identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX minimize setup errors and speed assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eE1B linear DNA Control:\u003c\/strong\u003e Supplied lyophilized linear DNA control for preparation of serial dilutions and construction of an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on multiple real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, qTOWER3G, LightCycler480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFor residual host cell DNA testing:\u003c\/strong\u003e Designed specifically to quantitate residual E1B DNA from HEK293 and derivative cell lines in biopharmaceutical samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1B DNA in biopharmaceutical products (in-process and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in HEK293 and derivative cell lines (e.g., 293T, 293F)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host cell DNA analysis\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA clearance and downstream process effectiveness\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B linear DNA Control (lyophilized powder × 1 tube) — Part No. NNA014\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes) — Part No. NNB002\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B Primer \u0026amp; Probe MIX (300 μL × 1 tube) — Part No. NNC034\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube) — Part No. NNC066\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes) — Part No. NND001\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free consumables and low-retention tips to avoid contamination and loss.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer\u0026amp;Probe MIX, IPC MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup to reduce contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure lyophilized E1B linear DNA Control is fully dissolved (add 55 μL ddH2O and mix as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 or undetermined; check IPC Ct to assess inhibition (sample IPC Ct must be within ±1.0 Ct of NCS IPC Ct).\u003c\/p\u003e\r\n \u003cp\u003e• Establish assay-specific LOD\/LOQ during method validation; lowest manual standard = 4.34 × 10^1 copies\/μL.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses fluorescent quantitative PCR (qPCR) for absolute quantitation of E1B DNA (FAM-labeled target, VIC-labeled IPC, ROX passive reference).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit and what is the recommended reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. The recommended total reaction volume is 30 μL (20 μL qPCR MIX + 10 μL sample\/control).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the standard curve range included in the user protocol and is LOQ specified?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol prepares a standard curve with concentrations from 4.34 × 10^6 down to 4.34 × 10^1 copies\/μL (ST1–ST6). A numeric LOQ\/LOD is not specified in the manual; users should determine assay-specific LOD\/LOQ during validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIPC (internal positive control) monitors PCR performance and sample inhibition. The Ct(IPC) of a sample should be within ±1.0 Ct of the Ct(IPC) of the NCS. The NTC should have Ct ≥ 35 or be undetermined. A significantly higher Ct(IPC) in a sample indicates possible inhibition; consider dilution or purification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173461713077,"sku":"1101110","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1B_DNA_Quantitation_Kit.png?v=1742280232"},{"product_id":"residual-pg13-dna-quantitation-kit-shentek®","title":"Residual PG13 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual PG13 DNA Quantitation Kit is designed to quantify residual PG13 host cell DNA in biopharmaceutical samples at different stages (in-process through final product). The kit uses quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with sensitivity at the femtogram (fg) level. The kit includes a PG13 DNA Control as a reference standard and reagents to prepare standard curves and perform absolute quantitation. For extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide. The kit is supplied for research use only and provides reagents sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (qPCR) using FAM-labeled probe\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to the femtogram (fg) level (analytical sensitivity stated as fg-level in kit documentation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (pg\/μL)\u003c\/td\u003e\r\n \u003ctd\u003e3000 (ST0 stock) and working standards ST1=300, ST2=30, ST3=3, ST4=0.3, ST5=0.03, ST6=0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (demonstrated by standards)\u003c\/td\u003e\r\n \u003ctd\u003eApproximately 300 to 0.003 pg\/μL (prepared standard series provides \u0026gt;5 orders of magnitude coverage from working standards)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePer-reaction reagent volumes (qPCR MIX)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + PG13 Primer\u0026amp;Probe MIX 3 μL (total 20 μL qPCR MIX per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Roche LightCycler 480 II (and other compatible real-time PCR systems supporting FAM detection and absolute quantitation mode)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02 (PG13-DNA detector); Auto baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger than mean Ct of lowest standard (or \u0026lt; QL when QL \u0026lt; lowest standard); verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C storage; kit components stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram (fg) level for detection of trace residual PG13 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA Control and a serial dilution standard curve to perform absolute quantitation (standard curve method).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e qPCR Reaction Buffer and PG13 Primer \u0026amp; Probe mix provided for streamlined plate setup and consistent reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Detailed instructions for serial dilutions, controls (ERC, PCS, NCS, NTC), qPCR mix preparation and recommended analysis parameters.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eResearch use supply:\u003c\/strong\u003e Supplied for Research Use Only (RUO) and includes reagents sufficient for 100 reactions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical process intermediates\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual PG13 DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA assays targeting PG13 cell line\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control monitoring of host cell DNA removal\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer \u0026amp; Probe MIX (NNC101) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C for working use as directed)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards and controls carefully; include at least five concentrations in the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before running the qPCR.\u003c\/p\u003e\r\n \u003cp\u003e• Follow recommended analysis settings (Manual Ct, Threshold 0.02, Auto Baseline) or validate settings per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• QC acceptance: ERC recovery 50%–150%; NTC should be ≥35 cycles or undetermined; NCS Ct must be higher than the lowest standard Ct (or meet QL criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses quantitative PCR (qPCR) with a FAM-labeled probe for absolute quantitation using a standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sensitivity can I expect from the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit documentation reports sensitivity at the femtogram (fg) level. Working standard series are provided down to 0.003 pg\/μL (3 fg\/μL), but laboratories should validate the limit of quantitation (QL) in their own workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reagents be prepared and loaded?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare qPCR MIX (17 μL qPCR Reaction Buffer + 3 μL Primer\u0026amp;Probe MIX per reaction), aliquot 20 μL per well and add 10 μL of sample or standard (total 30 μL reaction). Include NTC, NCS, PCS and ERC controls and run standards in triplicate. Seal plate, mix and centrifuge briefly before starting the run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should be ≥35 cycles or undetermined. NCS Ct should be larger than the mean Ct of the lowest standard or meet QL criteria determined in validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173516992693,"sku":"1101123","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Quantitation_Kit.png?v=1742289744"},{"product_id":"residual-mrc-5-dna-quantitation-kit-shentek®","title":"Residual MRC-5 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MRC-5 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual MRC-5 host cell DNA in biopharmaceutical samples at in-process and final product stages. The kit enables rapid, specific, and reliable absolute quantitation down to the femtogram (fg) level using an included MRC-5 DNA Control as reference standard. The kit is supplied as reagents for 100 reactions (Product No.: 1101124) and is intended for research use only. For sample extraction guidance, refer to the corresponding SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MRC-5 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (FAM reporter)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ analytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level; lowest standard used 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eStandard points: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection value of NTC should be ≤ 6.00 fg\/µL (or laboratory-specific criteria after method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction mix per test\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: qPCR Reaction Buffer 17 µL + MRC-5 Primer \u0026amp; Probe MIX 3 µL (prepare 20 µL qPCR MIX then add 10 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95°C 10:00 (1 cycle); Denaturation: 95°C 0:15 (40 cycles); Annealing\/extension and read: 60°C 1:00 (40 cycles)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 example provided); compatible with standard real-time PCR instruments\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format and size\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions; Product No. 1101124; Version A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (individual components stored at -20 °C as labeled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eResult reporting units\u003c\/td\u003e\r\n \u003ctd\u003eMean Quantity reported in pg\/µL (results exportable from instrument software)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ acceptance\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard; NTC detection value ≤ 6.00 fg\/µL; ERC recovery 50–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to the femtogram level (lowest standard 0.03 pg\/µL = 30 fg\/µL) enabling detection of trace residual host-cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes MRC-5 DNA Control for construction of a standard curve and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe mix simplify setup and reduce preparation variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example settings provided for common real-time PCR platforms (ABI 7500, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Appropriate for in-process and final product testing, and for method validation and routine QC of residual host cell DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MRC-5 host-cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product QC testing for host-cell DNA content\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery assessment (using ERC samples)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 DNA Control (NNA062) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 Primer \u0026amp; Probe MIX (NNC118) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (remaining unused DDB store at 2–8 °C after thaw; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Read Material Safety Data Sheets (MSDS) before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use RNase\/DNase-free consumables and low-retention tips; follow sterile technique.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points; perform method validation to select sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix on microplate shaker and briefly centrifuge before running.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50–150%; NCS Ct should be larger than the mean Ct of the lowest standard; NTC signal ≤ 6.00 fg\/µL (or lab-defined criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to detect residual MRC-5 DNA at the femtogram (fg) level. The lowest standard provided is 0.03 pg\/µL (30 fg\/µL). The NTC acceptance criterion is ≤ 6.00 fg\/µL; final LOD should be established during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are supplied in a single kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supplies reagents for 100 reactions (Product No. 1101124).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What instrument settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings for ABI 7500 (SDS v1.4) are provided: 30 µL reaction volume; Activation 95°C 10:00; 40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C. If using other instruments, adapt thermal profile and settings according to the instrument documentation and validate accordingly.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store kit components and how long are they stable?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C as labeled. The kit components can be stored under appropriate conditions for up to 24 months. After thawing, store unused DNA Dilution Buffer (DDB) at 2–8 °C; if it becomes cloudy, incubate at 37 °C until clear.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175198253237,"sku":"1101124","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MRC-5_DNA_Quantitation_Kit.png?v=1742289936"},{"product_id":"residual-bovine-dna-quantitation-kit-shentek®","title":"Residual Bovine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 b0C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK4 Residual Bovine DNA Quantitation Kit is designed to quantitate residual bovine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The assay provides rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit includes bovine DNA control, primer \u0026amp; probe mix, internal positive control (IPC) mix, qPCR reaction buffer and DNA dilution buffer. Compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500). For sample extraction recommendations refer to the SHENTEK Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). For Research Use Only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1101112\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical principle\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (kit description states fg-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Calibration range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared standards from 3000 pg\/bcl down to 0.03 pg\/bcl (ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 bL total reaction volume (20 bL qPCR MIX + 10 bL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 bL, Bovine primer \u0026amp; probe MIX 2.8 bL, IPC MIX 1.3 bL (total 20 bL); add 10 bL sample or standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG (optional) 25 b0C 10:00; Activation 95 b0C 10:00; 40 cycles of Denaturation 95 b0C 00:15 and Annealing\/Extension 63 b0C 00:40 (fluorescence read during 63 b0C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria (IPC)\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within b10.0 Ct of NCS (if significantly higher, sample inhibition suspected)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction Recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery rate acceptance range: 50% e2 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NCS\/NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NCS should be larger than mean Ct of the lowest standard; NTC Ct should be at least 2 cycles larger than mean Ct of lowest standard (or follow lab-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 settings example provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 b0C, kit components can be stored at appropriate conditions for up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 bL purified test sample or 10 bL standards\/DDB per reaction (per recommended workflow)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram level (fg), enabling detection of trace residual bovine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a standard-curve based absolute quantitation method with serially diluted bovine DNA controls.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control:\u003c\/strong\u003e Includes an IPC mix to monitor PCR inhibition and validate sample results.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR MIX:\u003c\/strong\u003e Pre-formulated reaction buffer and primer\/probe mix minimize setup steps and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (example settings provided for ABI 7500 and SHENTEK-96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual bovine DNA in biomaterials and biological raw materials\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics, bioprocess intermediates and medical device materials\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and lot release testing for products where bovine-derived contaminants must be monitored\u003c\/li\u003e\r\n \u003cli\u003eResearch applications assessing bovine DNA contamination and extraction recovery\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine DNA Control (NNA032) 100 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine Primer \u0026amp; Probe MIX (NNC042) 300 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 bL d7 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL d7 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination (UV-decontaminate surfaces and equipment, 75% ethanol disinfection).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2-8 b0C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light; include appropriate controls (NTC, NCS, ERC) and run at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer becomes cloudy, warm at 37 b0C until clear; store unused DDB at 2-8 b0C.\u003c\/p\u003e\r\n \u003cp\u003e• UNG treatment step is optional and should be included only if UNG enzyme is added to the reaction mix.\u003c\/p\u003e\r\n \u003cp\u003e• Follow IPC and ERC acceptance criteria: IPC Ct within b11.0 of NCS; ERC recovery 50% to 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) or limit of quantitation (LOQ) for the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for fg-level sensitivity. The provided standard curve ranges from 3000 pg\/bcl to 0.03 pg\/bcl; validated LOD\/LOQ should be established by the user in their laboratory during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR instrument settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings are provided for ABI 7500 (SDS v1.4): optional UNG 25 b0C 10:00; activation 95 b0C 10:00; 40 cycles of 95 b0C 00:15 and 63 b0C 00:40 (fluorescence read at 63 b0C). Adjust settings as needed for other platforms and software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the supplied Bovine DNA Control serially in DNA Dilution Buffer to generate standards: ST0 = 30000 pg\/bcl (stock) then ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl. Include at least five concentrations in the curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included in each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), Extraction Reference Control (ERC), and serial standards (ST1-ST6). Run samples and controls in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175224959157,"sku":"1101112","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Bovine_DNA_Quantitation_Kit.png?v=1742290060"},{"product_id":"residual-procine-dna-quantitation-kit-shentek®","title":"Residual Procine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Porcine DNA Quantitation Kit is designed to quantify residual porcine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The kit provides a rapid, specific and reliable absolute quantitation assay with sensitivity down to the femtogram (fg) level. The product is supplied for 100 reactions (Product No. 1101113) and is intended for research use only. For sample extraction instructions, refer to the SHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). The kit includes internal positive control (IPC) reagents and a porcine DNA standard control to build a standard curve for absolute quantitation.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual porcine DNA in biomaterials (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (kit optimized for absolute quantitation down to 0.03 pg\/µL standard point)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared: ST0 30000 pg\/µL (stock), ST1 3000 pg\/µL, ST2 300 pg\/µL, ST3 30 pg\/µL, ST4 3 pg\/µL, ST5 0.3 pg\/µL, ST6 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 µL; Porcine Primer \u0026amp; Probe MIX 2.8 µL; IPC MIX 1.3 µL; (optional UNG enzyme 0.1 U)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eOptional UNG 25 °C 10:00; Activation 95 °C 10:00; 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 00:40 (annealing\/extension, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorophores \/ channels\u003c\/td\u003e\r\n \u003ctd\u003ePorcine-DNA: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for Porcine-DNA and 0.02 for IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of the NCS (negative control) IPC Ct; large shift indicates inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery criteria (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard concentration; NTC should be 2 cycles larger than mean Ct of lowest concentration (or laboratory-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months; key reagents stored at –20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System; other real-time PCR systems supported with appropriate setup\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Optimized qPCR assay capable of femtogram-level detection for low-abundance residual porcine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes porcine DNA control and recommended serial dilutions to generate an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IPC):\u003c\/strong\u003e IPC MIX included to monitor extraction and PCR inhibition for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated AT qPCR Reaction Buffer and Porcine Primer \u0026amp; Probe MIX reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarryover prevention option:\u003c\/strong\u003e Supports optional UNG treatment step when UNG enzyme is included to prevent carryover contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Detailed guidance for serial dilution, plate layout, thermal cycling and result analysis for reproducible assays.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual porcine DNA in biomaterials (e.g., tissue-derived products, implants, scaffolds)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for animal-derived material processing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and extraction recovery assessment using ERC samples\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of porcine DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine DNA Control (NNA031) 100 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 µL × 2\u003c\/span\u003e\u003cstrong\u003e–20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine Primer \u0026amp; Probe MIX (NNC043) 300 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation: User Guide (this manual)\u003c\/span\u003e\u003cstrong\u003eambient\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes by UV irradiation (30 minutes) and 75% ethanol prior to setup to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents at –20 °C; check expiration dates on labels. Protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations when preparing the standard curve; recommended serial dilution series provided (ST1–ST6).\u003c\/p\u003e\r\n \u003cp\u003e• Prepare NTC, NCS and ERC controls as instructed. Test samples and controls in triplicate for reliable results.\u003c\/p\u003e\r\n \u003cp\u003e• If UNG is used, include 25 °C 10:00 UNG step in the thermal program; UNG is optional but recommended to prevent carryover.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC Ct values to assess inhibition; acceptable IPC Ct shift is ±1.0 relative to NCS.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery rate is 50%–150%; if outside this range investigate extraction and inhibition.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively measure residual porcine DNA in biomaterials using qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 µL (20 µL qPCR MIX + 10 µL sample or standard). qPCR MIX per reaction: AT qPCR Reaction Buffer 15.9 µL, Porcine Primer \u0026amp; Probe MIX 2.8 µL, IPC MIX 1.3 µL. Optional UNG 0.1 U per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the provided Porcine DNA Control with DNA Dilution Buffer to produce a serial dilution series: ST0 30000 pg\/µL then ST1 3000, ST2 300, ST3 30, ST4 3, ST5 0.3, ST6 0.03 pg\/µL. Include at least five concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What criteria indicate assay inhibition or failure?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC mean Ct is \u0026gt; ±1.0 Ct from the NCS IPC Ct, the sample may be inhibitory. ERC recovery outside 50%–150% or abnormal NTC\/NCS Ct values also indicate possible issues; troubleshoot extraction, sample dilution, or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175394730165,"sku":"1101113","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dna-Residual_Procine_DNA_Quantitation_Kit.png?v=1742290235"},{"product_id":"residual-293t-rna-quantitation-kit-shentek®","title":"Residual 293T RNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual 293T RNA Quantitation Kit is a one-step reverse transcription and fluorescence quantitative PCR (one-step RT-qPCR) assay designed to quantitate residual total RNA from 293T host cells in biopharmaceutical products at various manufacturing stages (in-process samples to final products). The kit uses specific primers and probes for 293T RNA and contains an Internal Positive Control (IPC) to monitor reaction performance and inhibition. The assay achieves femtogram-level sensitivity and is intended for research use. For nucleic acid extraction guidance, refer to SHENTEK® Residual Host Cell RNA Sample Preparation Kit (Product No. 1201205).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (claimed)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects residual 293T total RNA at femtogram (fg) level; lowest standard in recommended curve = 0.002 pg\/µL (2 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended standard curve concentrations: 200 pg\/µL (ST0) \/ Example curve used in protocol: ST1=20 pg\/µL, ST2=2 pg\/µL, ST3=0.2 pg\/µL, ST4=0.02 pg\/µL, ST5=0.002 pg\/µL — effective linear range demonstrated from 2000 pg\/µL (starting control dilution) down to 0.002 pg\/µL depending on preparation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 µL total reaction volume (15 µL qRT-PCR mix + 5 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e5 µL purified sample (or 5 µL standard\/NTC\/NCS) per 20 µL reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription: 50 °C for 15:00 (1 cycle); Activation: 95 °C for 0:30 (1 cycle); 45 cycles of Denaturation 95 °C 0:10 and Annealing\/Extension 60 °C 0:40 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dyes \/ channels\u003c\/td\u003e\r\n \u003ctd\u003e293T-RNA: FAM reporter; RNA-IPC: VIC reporter; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt-IPC of sample and NCS should be within ±1.0 Ct; significantly higher Ct-IPC in sample indicates possible inhibition. If IPC of NCS or sample fails, evaluate inhibition and recovery.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be 2 cycles greater than mean Ct of the lowest standard; Ct of NCS should be larger than mean Ct of lowest standard and show VIC signal amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePlate layout \/ replicates\u003c\/td\u003e\r\n \u003ctd\u003eRecommended: at least five standard concentrations, NTC, NCS, IPC-NCS and IPC for each test sample; triplicate wells per sample\/standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stable up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus and other real-time PCR systems that support FAM\/VIC\/ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eUnits of report\u003c\/td\u003e\r\n \u003ctd\u003eResults reported as mean quantity in pg\/µL (per instrument result export)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOne-step RT-qPCR:\u003c\/strong\u003e Reverse transcription and qPCR in a single-step workflow to reduce handling and contamination risk.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram level (standards include down to 0.002 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC primer\/probe mix included to monitor reaction performance and detect sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-mixed primers \u0026amp; probes:\u003c\/strong\u003e 293T-specific primer \u0026amp; probe mix and RNA IPC primer \u0026amp; probe mix provided for convenience and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated with multiple real-time PCR instruments (SHENTEK-96S, ABI 7500, CFX96, Linegene 9600plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for standard curve quantitation:\u003c\/strong\u003e Includes 293T RNA Control and recommended serial dilution scheme for absolute quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual 293T host cell total RNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eTesting of in-process and final product samples for host cell RNA residues\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and sample recovery studies for residual host cell RNA\u003c\/li\u003e\r\n \u003cli\u003eQuality control during downstream processing and release testing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e293T RNA Control (NNA052) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e293T RNA Primer \u0026amp; Probe MIX (NNC096) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNA IPC Primer \u0026amp; Probe MIX (NNC053) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: One-step RT-qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent RNase contamination: use DNase\/RNase-free consumables, UV decontaminate work surfaces and tubes as described (30 min), disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (One Step qPCR Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Treat samples with DNase prior to detection to remove genomic DNA influence; optimize DNase digestion conditions for sample type.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five concentrations in the standard curve; run triplicates for standards and samples where possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: Ct-IPC for sample and NCS should be within ±1.0 Ct; significantly higher Ct-IPC indicates inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC and NCS acceptance criteria: NTC Ct should be ≥2 cycles above mean Ct of lowest standard; NCS Ct should be larger than mean Ct of lowest standard and show VIC signal.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201202.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201202.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents sufficient for 100 reactions (as stated on the product packaging).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 20 µL total reaction volume (15 µL qRT-PCR mix + 5 µL sample or control). Prepare qRT-PCR mix according to the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the thermal cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eReverse transcription 50 °C for 15:00, Activation 95 °C for 0:30, then 45 cycles of 95 °C 0:10 (denaturation) and 60 °C 0:40 (anneal\/extend; read fluorescence at 60 °C).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than IPC-NCS (greater than ±1.0 Ct difference), investigate inhibitors in the sample, consider sample dilution, re-extraction, or additional purification; prioritize recovery results if spike-in controls were run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181252337845,"sku":"1201202","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_293T_RNA_Quantitation_Kit.jpg?v=1742293531"},{"product_id":"car-tcr-transgene-copy-number-quantitation-kit","title":"CAR\/TCR Transgene Copy Number Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eSHENTEK® CAR\/TCR Transgene Copy Number Quantitation Kit is designed to quantify the copy number of target genes in human cell products derived from common HIV-1 lentiviral vectors (e.g., CAR or TCR genes integrated in CAR-T and TCR-T cell genomes). The kit uses a multiplex qPCR assay to detect DNA sequences related to the integration\/expression elements on the transfer plasmid and a Single Copy Gene (SCG) in human cells, enabling calculation of average copies of target gene per cell. The kit includes a company-produced reference standard and an internal positive control (IPC). The assay is intended for rapid, specific, reliable and universal lentiviral vector applications. For Research Use Only.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eProduct number\u003c\/td\u003e\n \u003ctd\u003eSK030221CA100\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReference standard initial concentration\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR\/TCR Control initial copy number: 2.33 × 10^8 copies\/μL\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStandard curve serial dilutions (copies\/μL)\u003c\/td\u003e\n \u003ctd\u003eST1: 2.33 × 10^6; ST2: 2.33 × 10^5; ST3: 2.33 × 10^4; ST4: 2.33 × 10^3; ST5: 2.33 × 10^2\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction volume\u003c\/td\u003e\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; CAR\/TCR Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReporter dyes \/ targets\u003c\/td\u003e\n \u003ctd\u003eCAR\/TCR target: FAM; SCG: CY5; IPC: VIC; ROX as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\n \u003ctd\u003eManual Ct threshold 0.02; Automatic baseline; verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria for IPC\u003c\/td\u003e\n \u003ctd\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; significant increase indicates inhibition\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eRecommended DNA input per well\u003c\/td\u003e\n \u003ctd\u003eRecommended 5 ng to 30 ng total DNA per well (adjust based on preliminary experiments)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCalculation formula\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample types\u003c\/td\u003e\n \u003ctd\u003ePurified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eMultiplex absolute quantitation:\u003c\/strong\u003e Simultaneous detection of CAR\/TCR target, single copy gene (SCG) and IPC in the same reaction for absolute copy number determination using a standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReference standard included:\u003c\/strong\u003e Proprietary reference standard supplied and pre-dilution scheme provided for a 5-point standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated CAR\/TCR Primer \u0026amp; Probe MIX and IPC MIX simplify assay setup and reduce pipetting error.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for use on SHENTEK-96S, ABI 7500, LineGene 9600plus, CFX96 and similar real-time PCR instruments.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eDesigned for lentiviral vector applications:\u003c\/strong\u003e Optimized for quantifying transgene copy numbers from HIV-1 derived lentiviral vectors (CAR\/TCR).\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQuantification of CAR or TCR transgene copy number in CAR-T and TCR-T cell products\u003c\/li\u003e\n \u003cli\u003eLentiviral vector integration assessment\u003c\/li\u003e\n \u003cli\u003ePreclinical and research cell therapy product characterization\u003c\/li\u003e\n \u003cli\u003eQuality control and batch testing for gene-modified cell products (research use)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Primer \u0026amp; Probe MIX (NNC032) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eIPC MIX (NNC066) 150 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C (store remaining DDB at 2–8 °C); -20 °C for long term per kit table\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Control (NNA022) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex absolute quantitation, standard curve)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean environment: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol to avoid contamination.\u003c\/p\u003e\n \u003cp\u003e• Avoid assay inhibitors; monitor IPC Ct — if sample IPC Ct is significantly higher than NCS IPC Ct, sample may be inhibitory.\u003c\/p\u003e\n \u003cp\u003e• NTC and NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/p\u003e\n \u003cp\u003e• Prepare and run triplicates for standards, NTC, NCS and test samples where possible.\u003c\/p\u003e\n \u003cp\u003e• Recommended sample input per well: 5 ng–30 ng total DNA (optimize as needed).\u003c\/p\u003e\n \u003cp\u003e• Store kit components as labeled (-20 °C) and protect from light; kit stable up to 24 months. If DDB is cloudy, heat to 37 °C until clear.\u003c\/p\u003e\n \u003cp\u003e• Seal plates\/tubes, mix and briefly centrifuge before running; follow instrument-specific setup and analysis instructions.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample type and amount should I use for the assay?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse purified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration. The recommended total DNA per well is between 5 ng and 30 ng; adjust based on preliminary experiments.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is transgene copies per cell calculated?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eCalculate CAR or TCR copies per cell using the formula: CAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG), where CAR\/TCR and SCG quantities are measured from the standard curve (copies\/μL).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15 (40 cycles); Annealing\/Extension 60 °C for 01:00 with fluorescence read during this step.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and control results?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; a significantly higher IPC Ct indicates possible inhibition. NTC and NCS Ct values should be larger than the mean Ct of the lowest standard.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181277864117,"sku":"SK030221CA100","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CARTCRTransgeneCopyNumberQuantitationKitSHENTEK.jpg?v=1757316229"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/RNA_DNA_Quantitation.png?v=1741714820","url":"https:\/\/biofargo.com\/collections\/rna-dna-quantitation-analysis.oembed?page=5","provider":"Biofargo","version":"1.0","type":"link"}