{"title":"Restriction Enzymes","description":"\u003ch2 style=\"text-align: center;\"\u003eRestriction Enzymes (Restriction Endonucleases)\u003c\/h2\u003e\n\u003cp\u003eRestriction enzymes, also known as restriction endonucleases, are essential tools in molecular biology. These enzymes recognize and cut DNA at specific nucleotide sequences, enabling researchers to manipulate DNA with high precision. Originally discovered in bacteria as a defense mechanism against viruses, restriction enzymes are now indispensable in cloning, genome editing, forensic analysis, and diagnostics.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eHow Restriction Enzymes Work\u003c\/h2\u003e\n\u003cp\u003eRestriction enzymes identify short, specific DNA sequences (recognition sites), usually 4–8 base pairs long. Upon recognition, they cleave the DNA by hydrolyzing the phosphodiester bonds within or near these sequences. This cleavage generates DNA fragments, either with \u003cstrong\u003eblunt ends\u003c\/strong\u003e or \u003cstrong\u003esticky ends\u003c\/strong\u003e:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBlunt-end cuts:\u003c\/strong\u003e Produce straight cuts through both DNA strands at the recognition site, yielding flat ends.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSticky-end cuts:\u003c\/strong\u003e Generate overhanging single-stranded ends, which can easily anneal with complementary sequences.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eTo prevent self-cleavage, host bacteria use \u003cstrong\u003emethylases\u003c\/strong\u003e to methylate their own DNA at the recognition sites, forming a restriction-modification system that distinguishes foreign DNA from self.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eTypes of Restriction Enzymes\u003c\/h2\u003e\n\u003cp\u003eRestriction enzymes are categorized based on their structure and cleavage patterns:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eType I: \u003c\/strong\u003eLarge multi-subunit complexes that cut DNA far from their recognition sites; require ATP and S-adenosyl methionine. Example: EcoKI.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eType II: \u003c\/strong\u003eMost widely used in labs. Cut DNA at or near the recognition site. Do not require ATP. Examples: EcoRI, HindIII, BamHI.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eType III:\u003c\/strong\u003e Multi-subunit enzymes requiring two recognition sites and ATP. Cut DNA at a defined distance away. Examples: EcoP15, EcoP1.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 style=\"text-align: left;\"\u003eApplications of Restriction Enzymes\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGene cloning \u003c\/strong\u003eand plasmid construction\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDNA fingerprinting\u003c\/strong\u003e in forensics\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDiagnostic assays \u003c\/strong\u003efor genetic disorders\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGenome editing\u003c\/strong\u003e, including CRISPR applications\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLibrary construction\u003c\/strong\u003e and genome mapping\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 style=\"text-align: left;\"\u003eChoosing the Right Enzyme\u003c\/h2\u003e\n\u003cp\u003eWhen selecting a restriction enzyme for DNA digestion, consider:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecognition site: \u003c\/strong\u003eEnsure the enzyme targets the exact DNA sequence of interest.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnd type: \u003c\/strong\u003eChoose between sticky or blunt ends depending on downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBuffer compatibility:\u003c\/strong\u003e Especially important in multi-enzyme reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation sensitivity: \u003c\/strong\u003eSome enzymes can't cleave methylated DNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStar activity: \u003c\/strong\u003eAvoid enzymes prone to off-target cleavage under suboptimal conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTemperature and reaction time: \u003c\/strong\u003eFollow recommended conditions for optimal activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 style=\"text-align: left;\"\u003eTips for Successful Digestion\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMaintain glycerol concentration below 5% in the reaction mix.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse the correct buffer with necessary cofactors (e.g., Mg2+).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOptimize incubation time to avoid incomplete digestion or star activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore enzymes at recommended temperatures to preserve activity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 style=\"text-align: left;\"\u003eBioFargo Restriction Enzymes\u003c\/h2\u003e\n\u003cp\u003eAt BioFargo, we provide a curated selection of high-performance restriction enzymes, rigorously tested for purity, specificity, and lot-to-lot consistency. Our restriction enzyme portfolio is marketed under the \u003cstrong\u003eKleanAB\u003c\/strong\u003e brand, known for its superior quality and performance.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKleanAB™\u003c\/strong\u003e \u003cstrong\u003eRestriction Enzymes\u003c\/strong\u003e are designed to meet the needs of researchers in gene cloning, diagnostics, and high-throughput applications. Our catalog includes commonly used enzymes such as EcoRI, BamHI, HindIII, and unique enzymes for specialized needs.\u003cbr\u003eWe also offer:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFast-digest enzymes \u003c\/strong\u003efor rapid results in under 15 minutes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUniversal buffer systems\u003c\/strong\u003e for simplified reaction setup\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-stability formats \u003c\/strong\u003efor extended shelf life\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cstrong\u003eExamples of specialized enzymes:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 89.9255%; border-collapse: collapse; border-style: none;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003eEnzyme\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eAbsI\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eCC^TCGAGG\u003cbr\u003eGGAGCT^CC\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eArthrobacter sp.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eFaiI\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eYA^TR\u003cbr\u003eRT^AY\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eFlavobacterium aquatile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eBarI\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eComplex site\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eBacillus sphaericus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003ePsrI\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003eComplex site\u003c\/td\u003e\n\u003ctd style=\"width: 33.3726%; text-align: left; vertical-align: middle; border-style: none;\"\u003ePseudomonas stutzeri\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eExplore our full enzyme catalog to find the ideal tool for your molecular biology applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHave questions or need help selecting an enzyme? Contact our technical team—we’re happy to help!\u003c\/strong\u003e\u003c\/p\u003e","products":[{"product_id":"supercut™-xhoⅰ-biosci","title":"XhoI-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…C↓T C G A G…3'\u003cbr\u003e3'…G A G C T↑C…5'\u003cbr\u003eIsoschizomers*: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231206151502_160x160.png?v=1701846927\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ XhoⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™XhoⅠ.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ AvrII was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ XhoⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria- Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be\u003cbr\u003eexpressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eTo 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003eTo 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eTo 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently suck or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃ or add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eimpaired\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"5000 units","offer_id":43541335736501,"sku":"8052441","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052441SuperCut_XhoIFastRestrictionEndonucleasespng.png?v=1732886496"},{"product_id":"supercut™-ndeⅰ-biosci","title":"NdeⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...C A↓T A T G...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...G T A T ↑A C...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: FauNDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206161534_160x160.png?v=1701850606\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NdeⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ NdeⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ NdeⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NdeⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NdeⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NdeⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NdeⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™  NdeⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"2000 units","offer_id":43541352087733,"sku":"8052251","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_91317f02-dc20-4d8e-823b-55878e93d954.jpg?v=1753176201"},{"product_id":"supercut™-nsiⅰ","title":"NsiⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...A T G C A↓T...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...T↑A C G T A...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: EcoT22I, Mph1103I, Zsp2IDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206165333_160x160.png?v=1701852836\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ NsiⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 1 μL of SuperCut™ NsiⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NsiⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NsiⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NsiⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NsiⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e14\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e9\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500 units","offer_id":43541364605109,"sku":"8052291","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052291SuperCut_NsiIpng.png?v=1732878695"},{"product_id":"supercut™-hindⅲ-biosci","title":"HindⅢ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…A↓A G C T T…3'\u003cbr\u003e3'…T T C G A↑A…5'\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206170842_160x160.png?v=1701853741\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ HindⅢ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ HindⅢ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ HindⅢ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ HindⅢ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ HindⅢ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ HindⅢ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e12\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eSome impaired\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"10000 units","offer_id":43541383184565,"sku":"8052181","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052181SuperCut_Hind_FastRestrictionEndonucleasespng.png?v=1732874002"},{"product_id":"speedycut-dpni-kleanab","title":"SpeedyCut DpnI-KleanAB","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eSpecification\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 234px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFull name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSpeedyCut DpnI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFast enzymatic digestion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSummary\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFeatures\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComposition\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecognition Site\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5'…-GA m6↓TC-…3' 3'…-C T↑A m6G-…5'\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eReaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e10X SpeedyOne Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecommended Reaction Temperature\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eUnit Definition\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eMethylation Effects\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eDam: Cannot cleave dam– DNA; Dcm: Methylation does not affect;CpG: Cleavage is blocked.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eThermal Inactivation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFast enzymatic digestion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e-20°C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Sangon","offers":[{"title":"50 RXNS","offer_id":43838990319797,"sku":"P310013-0050","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310013_1.jpg?v=1732883306"},{"product_id":"bsmbi","title":"BsmBI","description":"\u003ch2 class=\"MsoNormal\"\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1000 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBsmBI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eIt is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBsmBI is the isoschizoid enzyme of the SpeedyCut series restriction enzyme Esp3I, but because the reaction buffer is optimized to maximize the activity of BsmBI endonuclease, it becomes an independent system Buffer, which cannot be shared with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eStability is better than that of isoschizozyme Esp3I for a large number of linearized plasmid preparations.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1ul BsmBI,1X BsmBI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CGTCTC(N)1↓…-3' \u003cbr\u003e3'…-GCAGAG(N)5↑…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e1X BsmBI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e55°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOne unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 5 μl reaction system at 5℃ for 1 hour.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences overlap completely, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage may be blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 8°C for 2 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310001_manual_624855c5-e0b8-467f-99a2-ffc3a90fef40.pdf?v=1753412582\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310001_MSDS.pdf?v=1753412513\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1000U","offer_id":52400957751477,"sku":"P310001-1000U","price":141.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_bsmbl.jpg?v=1751472363"},{"product_id":"bspqi","title":"BspQI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 U\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eIt is commonly used in experiments such as Golden Gate assembly, rapid cloning, and preparation of a large number of linearized plasmids.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI is the isoschizome of the SpeedyCut series restriction enzyme SapI, but because the reaction buffer is consistent with the BsmBI enzyme reaction system, the BspQI endonuclease can exert maximum activity, so it can be shared with the B600199 reaction buffer, but not with the general SpeedyOne Buffer, and the recombinant albumin component is added to the reaction buffer, which greatly improves the stability of the enzyme\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eBspQI is a type of Type IIs endonuclease that recognizes non-palindromic sequences and cuts them outside of the recognition sequence\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50ul BspQI, 10X BspQI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GCTCTTC(N)1↓…-3' 3'…-CGAGAAG(N)4↑…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X BspQI Buffer (Dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOne unit of activity refers to the amount of enzyme required to completely digest 1 μg of λDNA in a 50 μl reaction system at 50℃ for 1 hour.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50ul BspQI, 10X BspQI buffer (dedicated)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003e\n\u003cspan\u003e\u003c\/span\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310002_manual.pdf?v=1753412653\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310002_MSDS.pdf?v=1753412727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500U","offer_id":52400957817013,"sku":"P310002-500U","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_bspql.png?v=1751472361"},{"product_id":"speedycut-sfii","title":"SpeedyCut SfiI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SfiI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GGCCNNNN↓NGGCC-…3' 3'…-CCGGN↑NNNNCCGG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 50°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Completely overlapping sequences, cleavage blocked; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310003_manual.pdf?v=1753413208\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310003_MSDS.pdf?v=1753413162\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400957882549,"sku":null,"price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-pspg-i","title":"SpeedyCut PspG I","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PspG I\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-↓CCWGG-…3' 3'…-GGWCC↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e75°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 75°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA（Dcm-）in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Sequences overlap completely, cleavage blocked; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_EN_P.pdf?v=1752658123\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_manual.pdf?v=1753413684\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310004_MSDS.pdf?v=1753413708\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400957948085,"sku":"P310004-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-agei","title":"SpeedyCut AgeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AgeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CCGGT-…3' 3'…-TGGCC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg p615 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences overlap completely, cleavage blocked; EcoKI:Methylation does not affect; EcoBI:Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310005_manual.pdf?v=1753413996\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310005_MSDS.pdf?v=1753413970\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400958013621,"sku":"P310005-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-apalⅰ","title":"SpeedyCut ApaLⅠ","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut ApaLⅠ\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5′-G↓TGCAC-3′ 3'-CACGT↑G-5′\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e150 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310006_manual.pdf?v=1753414295\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310006_MSDS.pdf?v=1753414315\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"150 RXNS","offer_id":44611372908725,"sku":"P310006-150 RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-asci","title":"SpeedyCut AscI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AscI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-GG↓CGCGCC-3' 3'-CCGCGC↑GG-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_manual.pdf?v=1753415117\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_MSDS.pdf?v=1753345000\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310007_MSDS.pdf?v=1753415101\" type=\"application\/pdf\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982327477,"sku":"P310007-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bamhi","title":"SpeedyCut BamHI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BamHI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-G↓GATCC-3' 3'-CCTAG↑G-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eOnly a small fraction can be inactivated by heating at 80°C for 20 minutes, and most of them cannot be thermoinactivated, so use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400982294709,"sku":"P310008-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bcli","title":"SpeedyCut BclI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BclI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-T↓GATCA-3' 3'-ACTAG↑T-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80°C for 20 min to lose activity\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e125 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310009_manual.pdf?v=1753415983\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310009_MSDS.pdf?v=1753416010\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"125RXNS","offer_id":52400982261941,"sku":"P310009-125RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bglii","title":"SpeedyCut BglII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BglII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓GATCT-…3' 3'…-TCTAG↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310010_manual.pdf?v=1753768852\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310010_MSDS.pdf?v=1753768409\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400982229173,"sku":"P310010-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-bsteii","title":"SpeedyCut BstEII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut BstEII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓GTNACC-…3' 3'…-CCANTG↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect;CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Sequences may overlap and cleavage blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocumnets\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100011_manual.pdf?v=1753769169\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100011_MSDS.pdf?v=1753769120\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400982196405,"sku":"P310011-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-clai","title":"SpeedyCut ClaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut ClaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AT↓CGAT-…3' 3'…-TAGC↑TA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P3100012_manual.pdf?v=1753769280\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310012_MSDS.pdf?v=1753769319\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982163637,"sku":"P310012-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-dpnii","title":"SpeedyCut DpnII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut DpnII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-↓GATC-…3' 3'…- CTAG↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocumnets\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310014_manual.pdf?v=1753772780\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310014_MSDS.pdf?v=1753772733\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400982130869,"sku":"P310014-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-eagi","title":"SpeedyCut EagI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EagI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓GGCCG-…3' 3'…-GCCGG↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310015_manual.pdf?v=1753773225\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310015_MSDS.pdf?v=1753773260\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400982098101,"sku":"P310015-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ecori","title":"SpeedyCut EcoRI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EcoRI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'-G↓AATTC-3' 3'-CTTAA↑G-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e600 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"600RXNS","offer_id":52400982065333,"sku":"P310016-600RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ecorv","title":"SpeedyCut EcoRV","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut EcoRV\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GAT↓ATC-…3' 3'…-CTA↑TAG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310017_manual.pdf?v=1753773392\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310017_MSDS.pdf?v=1753773422\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981999797,"sku":"P310017-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-fspi","title":"SpeedyCut FspI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut FspI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TGC↓GCA-…3' 3'…-ACG↑CGT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked when sequences overlap; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310018_manual.pdf?v=1753773505\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310018_MSDS.pdf?v=1753773530\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981934261,"sku":"P310018-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hindiii","title":"SpeedyCut HindIII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HindIII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓AGCTT-…3' 3'…-TTCGA↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310019_MSDS.pdf?v=1753774476\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981901493,"sku":"P310019-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hinfi","title":"SpeedyCut HinfI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HinfI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓ANTC-…3' 3'…-CTNA↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310020_manual.pdf?v=1753773935\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310020_MSDS.pdf?v=1753773927\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981868725,"sku":"P310020-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-hpai","title":"SpeedyCut HpaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut HpaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GTT↓AAC-…3' 3'…-CAA↑TTG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310021_manual.pdf?v=1753773997\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310021_MSDS.pdf?v=1753774015\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981835957,"sku":"P310021-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-kpni","title":"SpeedyCut KpnI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut KpnI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GGTAC↓C-…3' 3'…-C↑CATGG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310022_manual.pdf?v=1753774147\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310022_MSDS.pdf?v=1753774132\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981803189,"sku":"P310022-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-mlui","title":"SpeedyCut MluI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut MluI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CGCGT-…3' 3'…-TGCGC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310023_manual.pdf?v=1753774246\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310023_MSDS.pdf?v=1753774246\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981770421,"sku":"P310023-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-mnli","title":"SpeedyCut MnlI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut MnlI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCTC(N)7↓-…3' 3'…-GGAG(N)6↑-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310024_manual.pdf?v=1753774563\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310024_MSDS.pdf?v=1753774577\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981737653,"sku":"P310024-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ncoi","title":"SpeedyCut NcoI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NcoI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CATGG-…3' 3'…-GGTAC↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310025_manual.pdf?v=1753774687\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310025_MSDS.pdf?v=1753774669\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"30RXNS","offer_id":52400981704885,"sku":"P310025-30RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-ndei","title":"SpeedyCut NdeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NdeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CATGG-…3' 3'…-GGTAC↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310026_manual.pdf?v=1753774909\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310026_MSDS.pdf?v=1753774945\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981672117,"sku":"P310026-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nhei","title":"SpeedyCut NheI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NheI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓CTAGC-…3' 3'…-CGATC↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e30 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310027_manual.pdf?v=1753775119\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310027_MSDS.pdf?v=1753775106\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"30RXNS","offer_id":52400981639349,"sku":"P310027-30RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-noti","title":"SpeedyCut NotI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NotI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GC↓GGCCGC-…3' 3'…-CGCCGG↑CG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg N85 DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981606581,"sku":"P310028-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nspv","title":"SpeedyCut NspV","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NspV\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TT↓CGAA-…3' 3'…-AA↑GCTT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310029_manual.pdf?v=1753775953\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310029_MSDS.pdf?v=1753775954\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981573813,"sku":"P310029-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-nrui","title":"SpeedyCut NruI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut NruI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TCG↓CGA-…3' 3'…-AGC↑GCT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Sequences may overlap and cleavage blocked; Dcm: Methylation does not affect; CpG: Cleavage is blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310030_manual.pdf?v=1753776075\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310030_MSDS.pdf?v=1753776060\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981541045,"sku":"P310030-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-pvuii","title":"SpeedyCut PvuII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PvuII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CAG↓CTG-…3' 3'…-GTC↑GAC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310031_manual.pdf?v=1753776163\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310031_MSDS.pdf?v=1753776176\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981508277,"sku":"P310031-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-paci","title":"SpeedyCut PacI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PacI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-TTAAT↓TAA-…3' 3'…-AAT↑TAATT-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg pPacI DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310032_manual.pdf?v=1753776292\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310032_MSDS.pdf?v=1753776277\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981475509,"sku":"P310032-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-psti","title":"SpeedyCut PstI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut PstI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CTGCA↓G-…3' 3'…-G↑ACGTC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310033_manual.pdf?v=1753776420\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310033_MSDS.pdf?v=1753776417\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981442741,"sku":"P310033-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-saci","title":"SpeedyCut SacI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SacI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GAGCT↓C-…3' 3'…-C↑TCGAG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310034_manual.pdf?v=1753776507\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310034_MSDS.pdf?v=1753776529\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981409973,"sku":"P310034-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sali","title":"SpeedyCut SalI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SalI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-G↓TCGAC-…3' 3'…-CAGCT↑G-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310035_manual.pdf?v=1753776580\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310035_MSDS.pdf?v=1753776646\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981377205,"sku":"P310035-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sacii","title":"SpeedyCut SacII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SacII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCGC↓GG-…3' 3'…-GG↑CGCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg pScaII DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310036_manual.pdf?v=1753776738\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310036_MSDS.pdf?v=1753776740\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"60RXNS","offer_id":52400981344437,"sku":"P310036-60RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-spei","title":"SpeedyCut SpeI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 114px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SpeI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-A↓CTAGT-…3' 3'…-TGATC↑A-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg pUC19-SpeI DNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310037_manual.pdf?v=1753776855\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310037_MSDS.pdf?v=1753776839\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981311669,"sku":"P310037-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sbfi","title":"SpeedyCut SbfI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SbfI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCTGCA↓GG-…3' 3'…-GG↑ACGTCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310038_manual.pdf?v=1753776934\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310038_MSDS.pdf?v=1753776927\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981278901,"sku":"P310038-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-smai","title":"SpeedyCut SmaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SmaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-CCC↓GGG-…3' 3'…-GGG↑CCC-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 25°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage is blocked.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310039_manual.pdf?v=1753777020\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310039_MSDS.pdf?v=1753777015\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981246133,"sku":"P310039-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sphi","title":"SpeedyCut SphI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SphI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-GCATG↓C-…3' 3'…-C↑GTACG-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e50 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310040_manual.pdf?v=1753777116\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310040_MSDS.pdf?v=1753777114\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"50RXNS","offer_id":52400981213365,"sku":"P310040-50RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-sspi","title":"SpeedyCut SspI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut SspI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AAT↓ATT-…3' 3'…-TTA↑TAA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e60 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310041_manual.pdf?v=1753777203\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310041_MSDS.pdf?v=1753777201\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"60RXNS","offer_id":52400981180597,"sku":"P310041-60RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-stui","title":"SpeedyCut StuI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut StuI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-AGG↓CCT-…3' 3'…-TCC↑GGA-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Cleavage is blocked when sequences overlap; CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e100 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310042_manual.pdf?v=1753777284\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310042_MSDS.pdf?v=1753777283\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"100RXNS","offer_id":52400981147829,"sku":"P310042-100RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-taqi","title":"SpeedyCut TaqI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut TaqI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-T↓CGA-…3' 3'…-AGC↑T-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e65°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 65°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect;CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDo not heat inactivate, use phenol chloroform extraction or column purification.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e200 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310043_manual.pdf?v=1753778396\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310043_MSDS.pdf?v=1753778395\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"200RXNS","offer_id":52400981115061,"sku":"P310043-200RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-xbai","title":"SpeedyCut XbaI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut XbaI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-T↓CTAGA-…3' 3'…-AGATC↑T-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Cleavage is blocked when sequences overlap; Dcm: Methylation does not affect;CpG: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310044_manual.pdf?v=1753778727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310044_MSDS.pdf?v=1753778727\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981082293,"sku":"P310044-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-xhoi","title":"SpeedyCut XhoI","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut XhoI\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓TCGAG-…3' 3'…-GAGCT↑C-…5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Cleavage may be blocked when sequences overlap.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e500 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310045_manual.pdf?v=1753778817\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310045_MSDS.pdf?v=1753778819\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"500RXNS","offer_id":52400981049525,"sku":"P310045-500RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"},{"product_id":"speedycut-avrii","title":"SpeedyCut AvrII","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 RXNS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut AvrII\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFast enzymatic digestion\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSpeedyCut family of restriction enzymes is a series of fast enzymes that rapidly cleave DNA in less than 5-15 minutes. The SpeedyCut series of restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA through multiple stringent quality control steps.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAll SpeedyCut restriction enzymes are compatible with the universal SpeedyOne buffer, which can be combined in any combination within a unified reaction system, eliminating the need for multiple single digestions or buffer changes, making it convenient, stable, fast, and accurate to cleave vectors.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecognition Site\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e5'…-C↓CTAGG…-3' 3'…-GGATC↑C…-5'\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10X SpeedyOne Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRecommended Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e37°C\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eAt 37°C, in a 20 μl reaction system, 1 μl enzyme can completely digest 1μg λDNA in 15 minutes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eMethylation Effects\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eDam: Methylation does not affect; Dcm: Methylation does not affect; CpG: Methylation does not affect; EcoKI: Methylation does not affect; EcoBI: Methylation does not affect.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eThermal Inactivation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eHeat at 80 °C for 20 min to lose activity.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e25 ul enzyme, 10X SpeedyOne buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e-20°C\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310046_manual.pdf?v=1753779041\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310046_MSDS.pdf?v=1753779046\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"25RXNS","offer_id":52400981016757,"sku":"P310046-25RXNS","price":53.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_speedycut.png?v=1751472348"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/20250723_-27.jpg?v=1754287264","url":"https:\/\/biofargo.com\/collections\/restriction-enzymes.oembed?page=7","provider":"Biofargo","version":"1.0","type":"link"}