{"title":"Residual Host Cell Protein Quantitation","description":"\u003cp\u003eResidual Host Cell Protein (HCP) Quantitation kits provide robust tools for detecting trace levels of host cell–derived proteins using validated immunoassay platforms. These assays are widely used for process optimization, purification validation, and batch-to-batch consistency monitoring.\u003c\/p\u003e\n\u003cp\u003eHigh specificity, lot-to-lot consistency, and broad host cell coverage make these kits suitable for both early development and late-stage quality control testing.\u003c\/p\u003e","products":[{"product_id":"e-coli-protein-expression-strains-hcp-elisa-kit-one-step-elisa-shentek®","title":"E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    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30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 3 hours (primary incubation) + 15 minutes substrate incubation\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit); Anti-E.coli HCP-E Microtiter Strips 2-8 °C (stable for 60 days); Reconstituted Calibration Standard 2-8 °C (stable for 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is a one-step, solid-phase, double-antibody sandwich Enzyme-Linked Immunosorbent Assay (ELISA) intended to detect residual host cell proteins (HCPs) from E.coli protein expression strains (originated from BL21) in bioprocess samples. Polyclonal capture antibody (sheep anti-E.coli HCP affinity antibody) is pre-coated on microtiter strips. Calibration standards and samples are simultaneously incubated with HRP-conjugated anti-E.coli HCP detection antibody (one-step format). HRP catalyzes TMB oxidation to produce a colorimetric signal measured at 450 nm (with long-wavelength correction 620–650 nm). Concentrations are calculated by a 4-parameter logistic (4PL) fit to the calibration curve. The kit is supplied for research use only and is not intended for clinical diagnostic use.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat \/ Throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit; 8-well × 12 strips\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA with HRP\/TMB colorimetric detection (read at 450 nm, reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1.5–243 ng\/mL, R2 \u0026gt; 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration curve model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL); example parameters A=2.41626, B=1.09106, C=89.16833, D=0.01118, R2=0.99995 (example data)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, Vero, HEK293T, and Hansenula polymorpha HCPs (as tested)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 100 µL HRP detection reagent + 100 µL sample\/standard per well; incubate on microplate thermoshaker at 600 rpm for 3 hours at room temperature; wash; add 100 µL TMB and incubate 15 minutes at room temperature in dark; stop and read\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReading\u003c\/td\u003e\r\n                        \u003ctd\u003eAbsorbance measured at 450 nm with correction wavelength set at 620–650 nm; subtract NCS (blank) and use 4PL fit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (measurements may be compromised outside this range)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume for dilutions\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume 5 µL recommended for accurate dilutions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReading window after stop\u003c\/td\u003e\r\n                        \u003ctd\u003eComplete plate reading within 30 minutes after termination\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step ELISA format:\u003c\/strong\u003e Simultaneous addition of calibration standard\/sample and HRP-conjugated detection antibody reduces hands-on time and simplifies workflow.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ of 1.5 ng\/mL with a linear range up to 243 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStrain-targeted specificity:\u003c\/strong\u003e Polyclonal antibodies generated against E.coli protein expression strains (BL21 origin) to improve detection of residual HCPs from these expression hosts.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and supplied in sealed bag with desiccant for convenience and reproducibility.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated lack of cross-reactivity:\u003c\/strong\u003e No cross-reactivity observed with CHO, Vero, HEK293T, or Hansenula polymorpha HCPs (as tested).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Includes calibration standard, HRP-conjugated detection antibody, diluent, wash buffer concentrate, substrate, stop solution and sealing films.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in products expressed in E.coli BL21 protein expression strains\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process samples, harvested bulk, drug substance and drug product testing during downstream processing\u003c\/li\u003e\r\n                    \u003cli\u003eAssay development and validation for bioprocess monitoring of E.coli-expressed biologics (e.g., IL, rhIFN, rhGM-CSF, rhTNF, EGF\/FGF\/PDGF)\u003c\/li\u003e\r\n                    \u003cli\u003eSpike\/recovery and method validation studies for residual HCP measurement (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli HCP‑E Calibration Standard (PNB003), lyophilized (2 bottles)\u003c\/span\u003e\u003cstrong\u003eStore lyophilized at 2-8 °C; reconstitute with 500 µL Reconstitution Solution; reconstituted standard stable 2-8 °C for up to 30 days (single use recommended)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑E.coli HCP‑E Microtiter Strips (PNA003) 8‑well × 12 strips\u003c\/span\u003e\u003cstrong\u003eSealed with desiccant at 2-8 °C; store unused strips in bag with desiccant; stable for up to 60 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) 1 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; use for dilution of calibration standards, HRP (100×) and samples\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; dilute 1:10 with ultra-pure water before use; may crystallize at low temps—redissolve at 37 °C if necessary\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑E.coli HCP‑E:HRP (100×) (PNN001) 1 × 120 µL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; centrifuge before use; dilute 100× in Diluent to prepare 1× prior to assay; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND002) 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; equilibrate to room temperature 20 minutes before use; keep protected from light; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2-8 °C; corrosive—handle with eye\/skin protection\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature; keep clean\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step double-antibody sandwich ELISA (colorimetric HRP\/TMB)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (RUO); not intended for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile disposable tips, tubes and reservoirs; recommended to wipe bench and equipment with 75% ethanol before and after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra‑pure water; water temperature should not exceed 37 °C when preparing wash buffer.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted Calibration Standard and prepared 1× Anti‑E.coli HCP‑E:HRP are recommended for single use due to instability; prepare freshly before each experiment.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate should be colorless; discard and contact supplier if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid contact with eyes, skin and clothing; wear appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it will deactivate HRP and lead to underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended sample pH 6.5–8.5; outside this range results may be compromised.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for dilutions: 5 µL to ensure accuracy.\u003c\/p\u003e\r\n                    \u003cp\u003e• Seal microplate immediately after sample loading to prevent evaporation; avoid drying wells before substrate incubation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301301-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301301-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for detection of HCPs from any E.coli strain?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is specifically designed and validated for E.coli protein expression strains originated from BL21. Use with other E.coli strains requires method validation; the kit is not suitable for E.coli cloning strains or samples processed by alkaline lysis.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare and store the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstitute each lyophilized Calibration Standard bottle with 500 µL Reconstitution Solution, gently mix and let stand for ~5 minutes. Use to prepare serial dilutions as described. Reconstituted standard may be stored at 2–8 °C for up to 30 days but single use is recommended due to potential instability.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay LLOQ is 1.5 ng\/mL with a validated linear range of 1.5–243 ng\/mL (R2 \u0026gt; 0.99 in internal testing).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What sample types are compatible and how should they be prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCompatible sample types include in-process samples, harvested bulk, drug substance and drug product. Samples should be clear and transparent; remove particulates by centrifugation or filtration, avoid repeated freeze-thaw cycles, and adjust pH to within 6.5–8.5. Dilute samples into the calibration range and verify suitability using dilution series and spike recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45187324051637,"sku":"1301301-1","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E.coli_ProteinExpressionStrains_HCPELISAKit_One-stepELISA.jpg?v=1742276232"},{"product_id":"e-coli-k-12-alkaline-lysis-hcp-elisa-kit-shentek®","title":"E.coli (K-12 \u0026 Alkaline Lysis) HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store; strips: up to 60 days; reconstituted standard: up to 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA sandwich-format solid-phase ELISA for quantitation of residual host cell proteins (HCPs) derived from E.coli K-12 strains (e.g., DH5α, Top10, JM109) in plasmid DNA (pDNA) intermediates or bulk prepared by alkaline lysis. The kit uses an affinity-purified capture antibody (sheep polyclonal) raised against alkaline-lysis-derived E.coli K-12 HCPs to capture HCPs, a biotinylated detection antibody and streptavidin–HRP for signal generation. Detection is performed with TMB substrate (read at 450 nm after stop), and sample concentrations are calculated against a six-point calibration curve using a 4-parameter logistic (4-PL) regression. The kit is supplied as 96 tests (8 × 12 strips) and is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003e96-well microtiter strips (8 × 12 strips), precoated with sheep anti-E.coli HCP-AC capture antibody\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended sample types\u003c\/td\u003e\r\n                        \u003ctd\u003epDNA intermediates, in-process samples, drug substance and drug product prepared by alkaline lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 - 729 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL) regression. Example parameters: A = 3.94571; B = -0.57096; C = 1732.85755; D = -0.05957; R² = 0.99973\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, Vero, HEK293T, and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eSample pH should be between 6.5 and 8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical incubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 100 µL, 1 hour at RT with shaking (300–500 rpm) or 2 hours standing; Biotinylated antibody: 45 min at RT; Streptavidin-HRP: 15 min at RT; TMB: 15 min at RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ Reactions\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePart \/ Product No.\u003c\/td\u003e\r\n                        \u003ctd\u003eProduct No.: 1301302; Calibration Standard PNB002; Strips PNA002; other reagents PN* as labeled\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAlkaline-lysis‑targeted antibodies:\u003c\/strong\u003e Sheep polyclonal antibodies raised against HCPs derived from alkaline lysis of E.coli K-12, improving detection accuracy for alkalinely prepared pDNA samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSandwich ELISA sensitivity:\u003c\/strong\u003e Double-antibody sandwich format with biotin–streptavidin–HRP amplification enables a lower limit of quantitation of 3 ng\/mL and a wide dynamic range up to 729 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use precoated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and supplied in sealed bags with desiccant for convenient setup and reduced plate-coating variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit for routine testing:\u003c\/strong\u003e Includes calibration standard (lyophilized), reconstitution solution, wash buffer concentrate, biotinylated detection antibody, streptavidin‑HRP, TMB substrate and stop solution.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated for common cell lines:\u003c\/strong\u003e Demonstrated no cross-reactivity with common non-E.coli host cells (CHO, Vero, HEK293T, Hansenula polymorpha).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual E.coli K-12 host cell proteins in plasmid DNA (pDNA) intermediates produced by alkaline lysis\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of HCP clearance during pDNA purification\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product pDNA for residual E.coli HCPs (research use)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation support and spike-recovery experiments for pDNA analytics\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli HCP-AC Calibration Standard (PNB002) — lyophilized (3 bottles)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; reconstituted standard: 2-8 °C up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-AC Microtiter Strips (PNA002) — 8 well × 12 strips\u003c\/span\u003e\u003cstrong\u003eStore sealed in bag with desiccant at 2-8 °C; opened strips: up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) — 2 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) — 3 × 25 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; if crystalline, warm to 37 °C to dissolve before dilution\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-AC Biotinylated Conjugate (20×) (PNG002) — 1 × 600 μL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge briefly before use; prepare fresh 1× dilution before assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002) — 1 × 140 μL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge briefly before use; prepare fresh 1× dilution before assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND002) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003eKeep sealed, protected from light, store at 2-8 °C; equilibrate to RT 20 min before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) — 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; corrosive — handle with appropriate PPE\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 3 pieces\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for clinical or diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Intended for qualified technicians. Users should validate the assay for their sample matrix prior to routine use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples must be clear and free of insoluble material; centrifuge or filter before testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5; samples outside this range may give abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; for long-term storage, keep samples at −70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use ultra-pure water and ensure water temperature does not exceed 37 °C when preparing reagents.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wash wells thoroughly and avoid drying wells prior to substrate addition.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB must be colorless before use; read plate within 30 minutes after adding stop solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is 1 M HCl — avoid contact with skin and eyes; use appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inhibits HRP and will underestimate HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare biotinylated antibody and streptavidin-HRP dilutions fresh for each assay; recommended single-use for some prepared solutions.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301302.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301302.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is optimized for pDNA samples and intermediates prepared by alkaline lysis from E.coli K-12 strains (e.g., DH5α, Top10, JM109). Samples should be clear and within pH 6.5–8.5.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe lower limit of quantitation (LLOQ) is 3 ng\/mL and the assay is linear from 3 to 729 ng\/mL (R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I incubate the plate without a thermoshaker?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — if a microplate thermoshaker is not available, incubate samples standing for 2 hours at room temperature instead of 1 hour with shaking.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I handle reagents after opening?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore opened components at 2–8 °C as specified (e.g., strips in bag with desiccant up to 60 days; reconstituted standard up to 30 days). Prepare certain dilutions fresh and use single-use when recommended.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Not for clinical diagnostic use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45187364716725,"sku":"1301302","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E.coli_K-12_Alkaline_Lysis_HCP_ELISA_Kit.jpg?v=1742278564"},{"product_id":"cho-hcp-elisa-kit-one-step-elisa-shentek®","title":"CHO HCP ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable until expiration date)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eOne-step double-antibody sandwich ELISA for quantification of residual CHO host cell proteins (HCPs) in CHO-expressed biologics. The kit uses affinity-purified sheep anti-CHO HCP capture antibody coated on microtiter strips and an HRP-conjugated polyclonal anti-CHO HCP detection antibody. Calibration standards and HRP-labeled detection antibody are added simultaneously to the coated wells, incubated, washed, then developed with TMB substrate (HRP catalyzes oxidation of TMB). After stopping the reaction with 1 M HCl, absorbance is read at 450 nm (with 620–650 nm reference). OD450 values correlate with HCP concentration and sample concentrations are calculated from a 4-parameter logistic (4PL) standard curve. No special sample pretreatment required; dilute samples into the calibration range as needed.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA, 96-well format\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1–128 ng\/mL, R² \u0026gt; 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL) fit (example: A=5.81069, B=-0.97488, C=200.99833, D=-0.00435, R²=0.99999)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection\/Wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample incubation time\u003c\/td\u003e\r\n                        \u003ctd\u003e3 hours at room temperature (500–600 rpm recommended on thermoshaker)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSubstrate incubation time\u003c\/td\u003e\r\n                        \u003ctd\u003e15 minutes at room temperature (TMB)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with MDCK, Vero, HEK293T, E. coli, P. pastoris and Sf9 cells\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample types\u003c\/td\u003e\r\n                        \u003ctd\u003eCell culture fluid, in-process samples, drug substance and drug product (clear\/transparent samples; remove insolubles by centrifugation\/filtration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards\u003c\/td\u003e\r\n                        \u003ctd\u003e6-point calibration: 128, 64, 16, 4, 2, 1 ng\/mL plus NCS (0 ng\/mL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage of kit\u003c\/td\u003e\r\n                        \u003ctd\u003eStore kit at 2–8 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReconstituted standard stability\u003c\/td\u003e\r\n                        \u003ctd\u003eReconstituted CHO HCP Calibration Standard: store at 2–8 °C up to 30 days or ≤ -20 °C with no more than 3 freeze-thaw cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eOpened strip stability\u003c\/td\u003e\r\n                        \u003ctd\u003eAnti-CHO HCP microtiter strips: store sealed with desiccant at 2–8 °C for up to 60 days\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSheep-derived polyclonal antibodies:\u003c\/strong\u003e Affinity-purified sheep anti-CHO HCP capture antibody and sheep polyclonal detection antibody for broad coverage of CHO HCPs.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step assay workflow:\u003c\/strong\u003e Calibration standards and HRP-conjugated detection antibody are added simultaneously to coated wells to simplify setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity and broad dynamic range:\u003c\/strong\u003e LOQ 1 ng\/mL with linear range up to 128 ng\/mL and strong 4PL curve fit (R² \u0026gt; 0.99).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e No cross-reactivity with several common host cell lines and expression systems (MDCK, Vero, HEK293T, E. coli, P. pastoris, Sf9).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use plate strips:\u003c\/strong\u003e Pre-coated 8-well strips provided in a sealed bag with desiccant to reduce waste and allow flexible assay sizes.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStandardized calibration:\u003c\/strong\u003e Six standardized calibration points and recommended 4PL analysis make quantitation straightforward.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in CHO-expressed biologics (monoclonal antibodies, recombinant proteins, vaccines)\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of upstream and downstream purification steps\u003c\/li\u003e\r\n                    \u003cli\u003eRelease testing and process development research (research use only)\u003c\/li\u003e\r\n                    \u003cli\u003eSample suitability testing and spiking\/recovery studies for method validation\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003eStore sealed with desiccant at 2–8 °C; after opening store strips in bag with desiccant at 2–8 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO HCP Calibration Standard (lyophilized) (PNB005) 2 bottles\u003c\/span\u003e\u003cstrong\u003eReconstitute with 500 µL Reconstitution Solution; reconstituted standard: 2–8 °C up to 30 days or ≤ -20 °C (≤3 freeze-thaw cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) 1×1.5 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C (use to reconstitute calibration standard)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004) 2×25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) 2×25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; dilute 1:10 with ultra-pure water before use; may crystallize at low temperature—dissolve at 37 °C if needed\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO:HRP (100×) (PNN002) 1×120 µL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; dilute 100× with Diluent immediately before use; centrifuge before use; discard after experiment (prepare fresh)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND005) 1×12 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C; equilibrate to room temperature 20 min before use; keep away from light; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) 1×6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C; handle with care (corrosive)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature (for covering plate during incubation)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (One-step double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile disposable tips, tubes and reservoirs. Wipe bench and equipment with 75% ethanol as recommended.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted CHO HCP Calibration Standard and 1× Anti-CHO:HRP are unstable; prepare fresh and discard after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inactivates HRP and will underestimate HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl—avoid direct contact with eyes, skin, and clothing; follow appropriate safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommend sample pH between 6.5 and 8.5; extreme pH may compromise measurements.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples; long-term sample storage recommended at -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate TMB to room temperature for 20 minutes before use and protect from light during storage.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge Anti-CHO:HRP (100×) before dilution to avoid reagent loss; dilute 100× immediately prior to assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition; seal plate to prevent evaporation during incubation.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301304-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301304-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity and dynamic range of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit LLOQ is 1 ng\/mL with a linear quantification range of 1–128 ng\/mL (4PL fit, R² \u0026gt; 0.99).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can this kit detect HCPs from non-CHO host cells?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is specifically developed for CHO-derived HCPs. It has been tested with no cross-reactivity to MDCK, Vero, HEK293T, E. coli, P. pastoris and Sf9, so it is not intended to quantify HCPs from non-CHO hosts.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and standards be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSamples should be stored at ≤ -70 °C for long-term storage to avoid degradation and minimize freeze–thaw cycles. Reconstituted calibration standard: store at 2–8 °C up to 30 days or ≤ -20 °C with no more than 3 freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the key incubation times and temperatures for the assay?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSample\/standard incubation: 3 hours at room temperature on a thermoshaker (500–600 rpm). TMB substrate incubation: 15 minutes at room temperature. Read absorbance at 450 nm (reference 620–650 nm) after adding stop solution.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45188915298485,"sku":"1301304-1","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CHOHCPELISAKit_One-stepELISA_SHENTEK.jpg?v=1757316143"},{"product_id":"anti-cho-hcp-antibody-shentek®","title":"Anti-CHO HCP Antibody SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); -65 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eAffinity-purified polyclonal sheep IgG targeting CHO host cell proteins (HCPs). Immunogen: CHO HCPs produced from CHO K1 \u0026amp; CHO S fed-batch supernatant. Provided as an unconjugated liquid antibody in PBS without preservatives. Supplied at 1 mg size. Intended for HCP coverage evaluations and common immunoassays including Western Blot and ELISA. Maintain at 2–8 °C for short-term use; for long-term storage store below -65 °C. Valid for 12 months. For research use only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eChinese hamster (CHO)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost \/ Isotype\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody (unconjugated)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eConjugate\u003c\/td\u003e\r\n                        \u003ctd\u003eUnconjugated\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCHO HCPs produced by CHO K1 \u0026amp; CHO S fed-batch supernatant\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid (in PBS)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAffinity purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBuffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8 °C for short-term use; for long-term storage, store below -65 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct No.\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1301304-Ab01\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad CHO HCP Coverage:\u003c\/strong\u003e Polyclonal antibody raised against CHO K1 \u0026amp; CHO S fed-batch supernatant to provide broad detection of CHO host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity Purified:\u003c\/strong\u003e Affinity purification improves specificity and reduces background in immunoassays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eVersatile Applications:\u003c\/strong\u003e Validated use in HCP coverage evaluations, Western Blot and ELISA workflows.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use Liquid Format:\u003c\/strong\u003e Provided as a liquid in PBS without preservatives for flexible assay integration.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHost cell protein (HCP) coverage evaluation\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot\u003c\/li\u003e\r\n                    \u003cli\u003eELISA (including kit-based HCP ELISA)\u003c\/li\u003e\r\n                    \u003cli\u003eGeneral immunoassay development and validation\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO HCP Antibody, affinity-purified, unconjugated\u003c\/span\u003e\u003cstrong\u003eMaintain at 2-8 °C short-term; long-term store below -65 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eFormulation buffer: PBS\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProduct information \/ datasheet\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A | Method: Immunoassay (ELISA, Western Blot)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2–8 °C for short-term storage. For long-term storage store below -65 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Contains no preservatives; avoid repeated freeze–thaw cycles and contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use appropriate laboratory personal protective equipment (PPE) and follow institutional safety procedures when handling biological reagents.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate antibody performance (dilution, blocking, detection conditions) in your specific assay and sample matrix prior to use in critical experiments.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301304-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301304-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended storage for this antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMaintain at 2–8 °C for short-term use. For long-term storage, store below -65 °C. Avoid repeated freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Is this antibody conjugated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The antibody is supplied unconjugated (affinity-purified sheep IgG). Conjugation can be performed by the end user if required.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which applications is this antibody suitable for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis antibody is suitable for HCP coverage evaluations, Western Blot and ELISA. Users should optimize assay conditions for their specific workflows.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What was used as the immunogen?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe immunogen was CHO host cell proteins produced from CHO K1 and CHO S fed-batch supernatant.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); Not for diagnostic or therapeutic use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45188923588789,"sku":"1301304-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-CHO_HCP_Antibody_SHENTEK.jpg?v=1744013028"},{"product_id":"cho-k1-hcp-elisa-kit-one-step-elisa-shentek®","title":"CHO K1 HCP ELISA Kit (One-step ELISA)  SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit; opened strips stable for 60 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSolid-phase enzyme-linked immunosorbent assay (one-step double-antibody sandwich ELISA) for quantitative determination of residual host cell proteins (HCPs) originating from the CHO-K1 cell line. The assay uses an affinity-purified sheep polyclonal capture antibody coated on microtiter strips and an HRP-labeled anti-CHO-K1 HCP detection antibody added simultaneously with calibration standards or samples. TMB substrate is used for color development (blue at 655 nm; stopped reaction yields yellow product measured at 450 nm). Sample concentrations are calculated from a 4-parameter logistic dose-response calibration curve.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (pre-coated microtiter strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 - 300 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with Sf9, HEK 293T, E.coli BL21, P. pastoris HCP\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical read wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference\/correction 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/reagent incubation: 3 hours at room temperature on thermoshaker (600 rpm); TMB development: 15 minutes at RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH range\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommended pH 6.5–8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage (opened components)\u003c\/td\u003e\r\n                        \u003ctd\u003eMicrotiter strips in sealed bag with desiccant: 2–8 °C up to 60 days; Reconstituted calibration standard: aliquot and store ≤ -20 °C up to 60 days (avoid freeze-thaw)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic regression\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCHO-K1 specific detection:\u003c\/strong\u003e Sheep polyclonal antibodies raised against CHO-K1 HCPs provide specific capture and detection of residual CHO-K1 host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step ELISA workflow:\u003c\/strong\u003e Simultaneous addition of HRP-labeled detection antibody with standards\/samples simplifies setup and reduces handling steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-coated strips:\u003c\/strong\u003e Microtiter strips come pre-coated with affinity-purified capture antibody and packaged with desiccant for convenience and consistency.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive and wide dynamic range:\u003c\/strong\u003e Validated linear range 3–300 ng\/mL with LLOQ 3 ng\/mL and R² ≥ 0.990.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMinimal cross-reactivity:\u003c\/strong\u003e No detectable cross-reactivity with common alternative expression hosts (Sf9, HEK293T, E.coli BL21, P.pastoris).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual CHO-K1 host cell proteins in process intermediates\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of harvested bulk, drug substance and drug product derived from CHO-K1 cells\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and recovery\/spike studies for HCP measurement\u003c\/li\u003e\r\n                    \u003cli\u003eResearch QC and bioprocess monitoring in development labs\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO-K1 HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003eSealed bag with desiccant, store at 2-8 °C (stable up to 60 days after opening)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO-K1 HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003e2 bottles, reconstitute with 500 µL Reconstitution Solution; aliquot and store ≤ -20 °C (stable up to 60 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e1 × 1.5 mL, room temperature equilibration before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL, store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL, dilute 1:10 with ultra-pure water; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO-K1 HCP:HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL, affinity-purified HRP-conjugated sheep antibody; dilute 100× in Diluent before use; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL, protect from light; equilibrate to RT 20 min before use; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (sulfuric acid)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL, corrosive—avoid contact; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces, store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Intended for qualified laboratory personnel only.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate assay and sample dilution strategy before routine testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use single-use consumables; wipe work surfaces and equipment with 75% ethanol when appropriate.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples and standards; long-term sample storage recommended at ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB substrate should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is sulfuric acid — avoid contact with eyes, skin and clothing; follow chemical safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid sodium azide (NaN3) in samples or reagents (it inactivates HRP).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Wash Buffer fresh from 10× concentrate; ensure solutions are clear (heat to 37 °C to dissolve precipitate if needed).\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for dilutions: 5 µL to reduce error.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301305-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301305-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay range and sensitivity?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe validated linear range is 3–300 ng\/mL with an LLOQ of 3 ng\/mL (R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can this kit detect HCPs from non-CHO-K1 expression hosts?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is specifically designed for CHO-K1-derived HCPs; no cross-reactivity was observed with Sf9, HEK293T, E. coli BL21 or P. pastoris HCPs, so it is not recommended for quantifying HCPs from those hosts.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reconstituted standards be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLong-term sample storage is recommended at ≤ -70 °C to prevent degradation. Reconstituted calibration standards should be aliquoted and stored at ≤ -20 °C and used within 60 days; avoid repeated freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Are there any reagents or sample components to avoid?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAvoid sodium azide in samples or reagents (it will deactivate HRP). Do not use samples outside recommended pH range (6.5–8.5) as this may cause abnormal results.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45188943118517,"sku":"1301305-1","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CHO_K1_HCP_ELISA_Kit_One-step_ELISA.jpg?v=1742281933"},{"product_id":"mdck-hcp-elisa-kit-shentek®","title":"MDCK HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (unopened; opened components: strips 60 days, reconstituted standard 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis SHENTEK MDCK HCP ELISA Kit is a one-step, solid-phase, double-antibody sandwich ELISA for quantitative detection of residual host cell proteins (HCPs) from MDCK (Madin–Darby canine kidney) cells. A sheep polyclonal anti-MDCK HCP affinity-purified capture antibody is coated on microtiter strips and an HRP-conjugated sheep anti-MDCK HCP detection antibody (used simultaneously with standards\/samples) enables colorimetric detection with TMB\/H2O2 substrate. After incubation and washing, HRP catalyzes TMB oxidation (blue, max absorbance ~655 nm) and reaction is stopped to give a yellow product read at 450 nm (with 620–650 nm reference). Sample HCP concentrations are interpolated from a 4‑parameter logistic (4‑PL) calibration curve constructed from supplied calibration standards.\u003c\/p\u003e\n\u003cp\u003eDesigned for research use only (not for clinical use). Supplied as a 96-test kit with pre-coated microtiter strips, lyophilized calibration standard, HRP-conjugated detection antibody (100×), buffers and substrates. Typical application includes in-process testing and final product testing for MDCK-derived biologics including vaccines and influenza virus products.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit format \/ throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit (8 wells × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay principle\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA, colorimetric detection (TMB, read at 450 nm with 620–650 nm reference)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2–200 ng\/mL (4‑PL curve), R2 ≥ 0.990 (typical R2 reported 0.9994)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration\u003c\/td\u003e\r\n                        \u003ctd\u003eEight-point calibration curve (ST1–ST8) with 4‑parameter logistic (4‑PL) regression; example 4‑PL parameters: A=9.57058, B=-1.04748, C=399.32097, D=0.00304, R2=0.9994\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical OD data (reference)\u003c\/td\u003e\r\n                        \u003ctd\u003eExample OD450–620nm averages: 200 ng\/mL = 3.237; 100 ng\/mL = 1.931; 50 ng\/mL = 1.089; 25 ng\/mL = 0.610; 10 ng\/mL = 0.312; 5 ng\/mL = 0.210; 2 ng\/mL = 0.148; 1 ng\/mL = 0.133; 0 ng\/mL = 0.111\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with E. coli, Pichia pastoris, or Sf9 cell HCPs\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product from MDCK-based processes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (outside this range may cause abnormal results)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions (typical)\u003c\/td\u003e\r\n                        \u003ctd\u003eSimultaneous addition of 1×Anti-MDCK:HRP and samples\/standards; incubate on microplate thermoshaker at 600 rpm for 3 hours at room temperature; wash ×5; add TMB and incubate 15 minutes at RT; add stop solution and read\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReadout\u003c\/td\u003e\r\n                        \u003ctd\u003eAbsorbance at 450 nm with reference at 620–650 nm; OD450–reference used for curve fitting\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReagent stability (opened)\u003c\/td\u003e\r\n                        \u003ctd\u003eAnti‑MDCK HCP microtiter strips: store in bag with desiccant at 2–8 °C up to 60 days; reconstituted calibration standard: 2–8 °C up to 30 days\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step simultaneous detection:\u003c\/strong\u003e Detection antibody (HRP-conjugated) is added simultaneously with standards\/samples to simplify workflow and reduce steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 2 ng\/mL with a linear dynamic range up to 200 ng\/mL using 4‑PL fitting.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMDCK-specific antibody coverage:\u003c\/strong\u003e Sheep polyclonal affinity-purified antibodies raised against MDCK HCPs provide broad coverage of host cell proteins from MDCK cells.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with capture antibody in sealed bag with desiccant for ease of use and storage.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMinimal cross-reactivity:\u003c\/strong\u003e Demonstrated no cross-reactivity with common non-MDCK host cells (E. coli, P. pastoris, Sf9).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual host cell proteins (HCPs) in products manufactured with MDCK host cells\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing of vaccines and influenza virus products produced in MDCK cells\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring (harvested bulk, intermediates)\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of drug substance and drug product for MDCK-derived HCP contamination\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003eLyophilized; reconstitute with 500 μL Reconstitution Solution; reconstituted standard store 2–8 °C up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑MDCK HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips in sealed bag with desiccant; store at 2–8 °C; after opening store in bag with desiccant at 2–8 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e2 × 1.5 mL; room temperature equilibration before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultrapure water before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑MDCK:HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 μL HRP‑conjugated affinity‑purified sheep antibody; dilute 100× in Diluent before use; centrifuge before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, protect from light, equilibrate to RT before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; corrosive—avoid contact with skin\/eyes; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces; room temperature storage\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (One-step double-antibody sandwich, HRP\/TMB colorimetric)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit to room temperature for 20 minutes before use; return reagents to 2–8 °C after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh 1×Wash Buffer and 1×Anti‑MDCK:HRP prepared immediately before use; prepare Anti‑MDCK:HRP by diluting 100× concentrate in Diluent and use immediately.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use Wash Buffer Concentrate or Diluent if cloudy or contains precipitates; heat to 37 °C to dissolve if necessary.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles of samples; for long-term storage use ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples should be clear\/transparent; remove particulates by centrifugation or filtration prior to assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5 to avoid abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive. Avoid contact with skin, eyes, and clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid sodium azide (NaN3) in samples or buffers as it will inactivate HRP and lead to underestimated HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 30 minutes after stopping reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended minimum pipetting volume for accurate dilutions: 5 μL.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301308.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301308.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for clinical diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This kit is for Research Use Only (RUO) and is not intended for clinical diagnostic use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I reconstitute the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 500 μL of the provided Reconstitution Solution to the lyophilized MDCK HCP Calibration Standard, gently invert 3–5 times, let stand 5 minutes until clear. Use the reconstituted standard to prepare the ST1–ST8 dilution series as described in the manual.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay LLOQ is 2 ng\/mL and the validated dynamic range is 2–200 ng\/mL using a 4‑parameter logistic (4‑PL) calibration curve.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What precautions should I take for samples and reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eEnsure samples are free of particulates (centrifuge\/filtrate), maintain pH 6.5–8.5, avoid sodium azide, minimize freeze-thaw cycles, prepare 1× reagents fresh, and follow recommended incubation and washing steps carefully.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45188968775861,"sku":"1301308","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MDCK_HCP_ELISA_Kit.jpg?v=1742282635"},{"product_id":"anti-mdck-hcp-antibody-shentek®","title":"Anti-MDCK HCP Antibody SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); \u0026lt; -65 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eAffinity-purified polyclonal sheep IgG directed against host cell proteins (HCPs) from MDCK (Madin-Darby Canine Kidney) cells. Immunogen: MDCK HCPs produced by sonication cell lysis. Provided as an unconjugated liquid antibody in PBS without preservatives. Supplied at 1 mg total protein. Intended for research use applications including HCP coverage evaluations, Western blot and ELISA.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eCanis familiaris (dog)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost \/ Isotype\u003c\/td\u003e\r\n                        \u003ctd\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody (unconjugated)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eConjugate\u003c\/td\u003e\r\n                        \u003ctd\u003eUnconjugated\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMDCK HCPs produced by sonication cell lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAffinity Purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBuffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8 °C for short-term use. For long-term storage store below -65 °C.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad MDCK HCP Recognition:\u003c\/strong\u003e Polyclonal antibody raised against whole MDCK host cell protein lysate for broad coverage of HCP species.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity Purified:\u003c\/strong\u003e Affinity purification improves specificity and reduces background in immunoassays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eUnconjugated Format:\u003c\/strong\u003e Flexible for use with a variety of secondary detection systems (HRP, AP, fluorescent labels) and assay formats.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use Liquid:\u003c\/strong\u003e Supplied in PBS as a liquid preparation for immediate use in assays; no reconstitution required.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eNo Preservatives:\u003c\/strong\u003e Formulation without preservatives suitable for many downstream analytical workflows (store appropriately to prevent contamination).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluations\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot\u003c\/li\u003e\r\n                    \u003cli\u003eELISA\u003c\/li\u003e\r\n                    \u003cli\u003eGeneral research immunoassays against MDCK host cell proteins\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-MDCK HCP Antibody (Affinity-purified, unconjugated)\u003c\/span\u003e\u003cstrong\u003eMaintain at 2-8 °C for short-term; for long-term store below -65 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable (antibody reagent) | Method: Immunoassay (ELISA \/ Western Blot)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8 °C for short-term storage. For long-term storage, keep below -65 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; aliquot if necessary.\u003c\/p\u003e\r\n                    \u003cp\u003e• Contains no preservatives—use aseptic technique to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow institutional safety procedures for handling biological reagents and animal-derived materials.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301308-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301308-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What species was this antibody raised in and what is the isotype?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis antibody is a polyclonal raised in sheep and is of the IgG isotype.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What applications is this antibody validated for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe product is intended for HCP coverage evaluations and is suitable for Western blot and ELISA. Users should optimize conditions for their specific assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the antibody be stored and what is its shelf life?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore at 2-8 °C for short-term use. For long-term storage keep below -65 °C. The validated shelf life is 12 months.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is this product suitable for diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is for research use only (RUO) and is not intended for diagnostic purposes.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45189109874869,"sku":"1301308-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-MDCK_HCP_Antibody.jpg?v=1742285726"},{"product_id":"vero-hcp-elisa-kit-shentek®","title":"Vero HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    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flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (refer to label)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThe SHENTEK Vero HCP ELISA Kit is a one-step, solid-phase double-antibody sandwich ELISA for quantitation of host cell proteins (HCPs) derived from Vero cells in bioproducts (suitable for in-process samples, harvested bulk, drug substance and drug product produced by sonication cell lysis). The assay uses an affinity-purified sheep polyclonal capture antibody coated on microtiter strips and an HRP-conjugated sheep anti-Vero HCP detection antibody added simultaneously with standards or samples. HRP catalyzes TMB oxidation producing a colorimetric signal (read at 450 nm with correction 620–650 nm). HCP concentration is calculated from a 4-parameter logistic (4PL) calibration curve generated from supplied calibration standards.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of Vero cell-derived host cell proteins (HCPs) in bioproducts; Research Use Only (RUO)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size \/ total reactions\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3–243 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards (nominal concentrations)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 243 ng\/mL; ST2 81 ng\/mL; ST3 27 ng\/mL; ST4 9 ng\/mL; ST5 3 ng\/mL; ST6 1 ng\/mL; NCS 0 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity reported with E. coli, P. pastoris and Sf9 cells\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with correction 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation times\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/antibody incubation: 3 hours at room temperature (600 rpm recommended); TMB substrate incubation: 10 minutes at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash conditions\u003c\/td\u003e\r\n                        \u003ctd\u003e1× Wash Buffer, 340 µL per well, 5 washes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWorking volumes\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL 1×Anti-Vero:HRP per well + 100 µL sample or standard per well; TMB 100 µL\/well; Stop solution 50 µL\/well\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStop solution\u003c\/td\u003e\r\n                        \u003ctd\u003e1 M HCl (corrosive)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage (kit \u0026amp; components)\u003c\/td\u003e\r\n                        \u003ctd\u003eKit: 2–8 °C; Microtiter strips stored sealed with desiccant at 2–8 °C (up to 30 days after opening); Reconstituted Calibration Standard: -20 °C, freeze\/thaw ≤3 cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume recommended for accurate pipetting: 5 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eResult calculation\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL) fit of calibration curve; subtract NCS, use dilution factor to get sample concentration\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step assay design:\u003c\/strong\u003e Simultaneous addition of HRP-conjugated detection antibody with standards\/samples simplifies workflow and reduces hands-on steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Lower limit of quantitation (LLOQ) of 3 ng\/mL and linear range up to 243 ng\/mL (R² ≥ 0.990).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh antibody coverage (sheep polyclonal):\u003c\/strong\u003e Affinity-purified sheep polyclonal antibodies provide broad coverage of Vero-derived host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and packaged with desiccant for convenient setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit components:\u003c\/strong\u003e Includes calibration standards, diluent, wash buffer concentrate, HRP-conjugated detection antibody, TMB substrate and stop solution for a full assay.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e Manufacturer reports no cross-reactivity with common alternative host systems (E. coli, P. pastoris, Sf9).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual Vero cell host cell proteins (HCPs) in in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eAnalysis of harvested bulk material\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product for Vero HCP content\u003c\/li\u003e\r\n                    \u003cli\u003eAssay development and validation in bioprocess monitoring and purification workflow optimization (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero HCP Calibration Standard (PNB012) — lyophilized\u003c\/span\u003e\u003cstrong\u003eStore lyophilized; reconstitute with 500 µL Reconstitution Solution; reconstituted standard stored at -20 °C (freeze\/thaw ≤3 cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Vero HCP Microtiter Strips (PNA013) — 8-well × 12 strips\u003c\/span\u003e\u003cstrong\u003ePre-coated strips stored in vacuum bag with desiccant at 2–8 °C; after opening store sealed with desiccant at 2–8 °C (up to 30 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) — 1 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C (used only to dissolve calibration standard)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C (for dilution of standards, Anti-Vero:HRP and samples)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; dilute 10× with ultra-pure water before use; warm to 37 °C if crystallized\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Vero:HRP (100×) (PNN007) — 1 × 120 µL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; centrifuge before use; dilute 100× in Diluent to prepare 1× working solution; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND005) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C, sealed and protected from light; equilibrate to RT before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) — 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; handle as corrosive (avoid contact with eyes\/skin\/clothing)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature; use to cover plate during incubation to prevent evaporation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (one-step double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C; check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) — it will inactivate HRP and cause underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standards and Anti-Vero:HRP working solution are recommended for single use due to instability; prepare fresh before each experiment.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB substrate should be colorless before use; discard and contact provider if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is 1 M HCl — corrosive; avoid contact with eyes, skin, and clothing and follow appropriate safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5; values outside this range may cause abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; for long-term storage of samples, -70 °C recommended.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after termination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use ultra-pure or sterile water for dilutions and ensure temperature does not exceed 37 °C when preparing solutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for accurate dilution: 5 µL.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301309.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301309.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for clinical diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for Research Use Only (RUO) and is not for clinical or diagnostic use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 3–243 ng\/mL with an LLOQ of 3 ng\/mL and reported R² ≥ 0.990.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit and reconstituted standards be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the complete kit at 2–8 °C. Reconstitute calibration standard per instructions and store reconstituted standard at -20 °C (limit freeze\/thaw cycles to ≤3). Keep unused strips sealed with desiccant at 2–8 °C.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended incubation times and reading parameters?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIncubate samples with 1× Anti-Vero:HRP for 3 hours at room temperature (600 rpm recommended). After washing, incubate with TMB for 10 minutes at room temperature. Read absorbance at 450 nm with correction wavelength 620–650 nm.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189061083317,"sku":"1301309","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/VeroHCPELISAKit.jpg?v=1742286379"},{"product_id":"anti-vero-hcp-antibody-shentek®","title":"Anti-Vero HCP Antibody  SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term) (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK Anti-Vero HCP Antibody is a polyclonal sheep IgG raised against Vero host cell proteins (HCPs) produced by sonication cell lysis. The product is provided as an unconjugated, affinity-purified liquid antibody in PBS with no preservatives. It is intended for research applications including HCP coverage evaluations, Western blot and ELISA. Store at 2-8 °C for short-term use and below -65 °C for long-term storage. Validity period: 12 months.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eAfrican green monkey (Vero)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost \/ Isotype\u003c\/td\u003e\r\n                        \u003ctd\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody, unconjugated\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eVero HCPs produced by sonication cell lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAffinity purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage buffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd\u003eHCP coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eMaintain at 2-8 °C for short-term use. For long-term storage store below -65 °C.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity-purified polyclonal IgG:\u003c\/strong\u003e High-specificity sheep polyclonal IgG purified by affinity methods to enrich target-specific antibodies and reduce non-specific background.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eImmunogen derived from Vero HCPs:\u003c\/strong\u003e Antibody raised against Vero host cell proteins produced by sonication cell lysis to provide broad HCP recognition relevant to Vero-derived materials.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady for ELISA and Western blot:\u003c\/strong\u003e Unconjugated format suitable for HCP coverage evaluations, one-step ELISA development and Western blot detection with secondary reagents.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eLiquid formulation, preservative-free:\u003c\/strong\u003e Provided in PBS as a liquid preparation with no preservatives for downstream assay flexibility; requires appropriate cold storage.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStorage stability:\u003c\/strong\u003e Stable short-term at 2-8 °C; long-term storage below -65 °C; 12-month validated shelf life.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluations\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot (immunoblot)\u003c\/li\u003e\r\n                    \u003cli\u003eELISA (including development of HCP ELISA assays)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Vero HCP Antibody, affinity-purified (1 mg)\u003c\/span\u003e\u003cstrong\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStorage buffer: PBS\u003c\/span\u003e\u003cstrong\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A | Method: ELISA \/ Western Blot (antibody reagent)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8 °C for short-term storage. For long-term storage, store below -65 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Product contains no preservatives; use aseptic technique and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate antibody concentration and performance (titer, specificity, cross-reactivity) for each intended application.\u003c\/p\u003e\r\n                    \u003cp\u003e• Handle per institutional biosafety and chemical safety procedures.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301309-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301309-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended storage condition for this antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMaintain at 2-8 °C for short-term use. For long-term storage, store below -65 °C. The product has a 12-month validity period when stored appropriately.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Is the antibody conjugated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The antibody is provided unconjugated and is suitable for use with labeled secondary antibodies or conjugation as required.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which applications is this antibody validated for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis antibody is intended for HCP coverage evaluations, Western Blot and ELISA. Users should validate performance in their specific assay formats.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Does the product contain preservatives?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product contains no preservatives; handle with aseptic technique and aliquot to minimize freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45189111742645,"sku":"1301309-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-VeroHCPAntibody.jpg?v=1742287070"},{"product_id":"sf9-hcp-elisa-kit-shentek®","title":"Sf9 HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: 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font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit; check label for expiration)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eThis is a solid-phase, one-step double-antibody sandwich ELISA kit for quantitative detection of residual host cell proteins (HCPs) from Spodoptera frugiperda (Sf9) insect cells. A sheep polyclonal anti-Sf9 HCP antibody is coated on microtiter strips to capture HCPs; HRP-conjugated anti-Sf9 antibody is used as detection reagent. Calibration Standards and samples are added together with the HRP-conjugated antibody, followed by incubation, washing, TMB substrate development (HRP catalyzes oxidation of TMB producing color change) and stop solution addition. Absorbance is read at 450 nm with long-wavelength correction (620–650 nm). Concentrations are calculated using a 4-parameter logistic (4-PL) fit to the calibration curve. The kit is intended for research use only and is not for clinical diagnostic use.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (capture antibody coated strips; simultaneous addition of HRP-conjugated detection antibody and samples\/standards)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3–243 ng\/mL; 4-PL fit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL); example curve parameters provided (A, B, C, D) with R2≥0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve performance\u003c\/td\u003e\r\n                        \u003ctd\u003eExample R2 = 1.000 (reference data provided); reference standard concentrations: 0, 1, 3, 9, 27, 81, 243 ng\/mL with corresponding OD values\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with correction wavelength 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample + 1×Anti-Sf9:HRP incubation on thermoshaker: 3 hours at room temperature (600 rpm recommended); TMB substrate incubation: 30 minutes at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity reported with MDCK, Vero, HEK293T, CHO, E. coli and P. pastoris\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH range\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (samples outside this range may cause abnormal results)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash buffer\u003c\/td\u003e\r\n                        \u003ctd\u003eWash Buffer Concentrate (10×) supplied; dilute 1:10 with ultra-pure water to prepare 1×Wash Buffer; recommended 5 washes, 340 µL per well\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControl \u0026amp; replicates\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommend 2–3 replicates per sample and inclusion of NCS (negative control) and spiked recovery controls (SRC) for validation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStability of opened components\u003c\/td\u003e\r\n                        \u003ctd\u003eMicrotiter strips: store in bag with desiccant at 2–8 °C up to 30 days; reconstituted calibration standard: short-term 2–8 °C, long-term aliquot and store below -20 °C (avoid \u0026gt;3 freeze-thaw cycles)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSafety\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStop Solution is 1 M HCl (corrosive) — avoid contact with eyes, skin, clothing; avoid samples containing sodium azide (inactivates HRP)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step format:\u003c\/strong\u003e Simultaneous addition of HRP-labeled detection antibody and standards\/samples reduces hands-on steps and assay time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Low limit of quantification (LLOQ) of 3 ng\/mL with linear range up to 243 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad antibody coverage:\u003c\/strong\u003e Sheep polyclonal anti-Sf9 HCP antibody provides broad recognition of Sf9 host cell proteins; coverage assessed by mainstream methods.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified capture antibody in sealed bags with desiccant for convenience and reduced preparation time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e No cross-reactivity observed with common cell lines and microbial expression hosts tested (MDCK, Vero, HEK293T, CHO, E. coli, P. pastoris).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Contains standards, detection antibody (HRP), diluent, wash buffer concentrate, substrate (TMB), stop solution and sealing film for a full ELISA workflow.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual host cell proteins (HCPs) in products manufactured using Sf9 insect cells\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of recombinant proteins produced in Sf9 cells\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control of vaccines produced using baculovirus\/Sf9 systems\u003c\/li\u003e\r\n                    \u003cli\u003eResidual HCP measurement for recombinant AAV vectors produced in BEVS\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process samples, harvested bulk, drug substance and drug product testing during R\u0026amp;D\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 HCP Calibration Standard (PNB011)\u003c\/span\u003e\u003cstrong\u003eLyophilized; reconstitute with Reconstitution Solution; short-term 2–8 °C, aliquot and store \u0026lt; -20 °C for long-term (avoid \u0026gt;3 freeze-thaw cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Sf9 HCP Microtiter Strips (PNA012)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips; store sealed in bag with desiccant at 2–8 °C; use within 30 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002)\u003c\/span\u003e\u003cstrong\u003e2 × 1.5 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C; dilute 1:10 with ultra-pure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Sf9:HRP (100×) (PNN006)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; affinity-purified sheep antibody conjugated to HRP; centrifuge before use; dilute 100× in Diluent immediately before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, keep away from light; equilibrate to RT before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; corrosive — store at 2–8 °C and handle with appropriate PPE\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step sandwich ELISA (solid-phase; HRP\/TMB detection)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2–8 °C. Check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit components to room temperature (≈20 minutes) before use; return to 2–8 °C after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted Sf9 HCP Calibration Standard: short-term 2–8 °C; aliquot and store \u0026lt; -20 °C for long-term; avoid \u0026gt;3 freeze–thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Anti-Sf9:HRP freshly by diluting the 100× stock; use immediately.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless before use; discard if colored. Protect from light during substrate incubation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive; avoid contact with eyes, skin and clothing; use appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it will inactivate HRP and lead to underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh sterile\/ultra-pure water (temperature ≤37 °C) for buffer preparation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition and avoid bubbles when pipetting as they affect OD readings.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate the assay and sample dilution strategy before routine use; recommended for qualified technicians only.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use single-use consumables; wipe benches and equipment with 75% ethanol before and after use to reduce contamination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301312.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301312.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 3–243 ng\/mL with an LLOQ of 3 ng\/mL. Calibration is performed using a 4-parameter logistic (4-PL) curve.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the Sf9 HCP Calibration Standard be stored after reconstitution?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstituted standard can be stored short-term at 2–8 °C. For long-term storage, aliquot and store below -20 °C and avoid more than three freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this kit be used for samples from other host cells (e.g., CHO, HEK293)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is developed specifically for Sf9 host cell proteins. The kit was reported to show no cross-reactivity with MDCK, Vero, HEK293T, CHO, E. coli and P. pastoris, so it is not intended for quantifying HCPs from other host cell lines.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What precautions should be taken with sample buffers or additives?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAvoid samples containing sodium azide as it inactivates HRP. Ensure sample pH is between 6.5 and 8.5. If samples are turbid or contain particulates, clarify by centrifugation or filtration and validate sample dilutions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189111775413,"sku":"1301312","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Sf9_HCP_ELISA_Kit.jpg?v=1742287627"},{"product_id":"anti-sf9-hcp-antibody-shentek®","title":"Anti-Sf9 HCP Antibody SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n   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25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term, stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003ePolyclonal sheep IgG antibody raised against Sf9 host cell proteins (HCPs) produced by sonication cell lysis. Affinity-purified, unconjugated antibody supplied in PBS without preservatives. Supplied as 1 mg vial. Intended for HCP coverage evaluations and detection of Sf9-derived host cell proteins in assays such as ELISA and Western Blot. Maintain at 2-8 °C for short-term use; for long-term storage store below -65 °C. Validity period: 12 months. For research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSpodoptera frugiperda (Sf9)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost\/Isotype\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody (unconjugated)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eConjugate\u003c\/td\u003e\r\n                        \u003ctd\u003eUnconjugated\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSf9 HCPs produced by sonication cell lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAffinity purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBuffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8 °C for short-term use; for long-term storage store below -65 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad HCP Coverage for Sf9:\u003c\/strong\u003e Polyclonal antibody raised against whole Sf9 HCPs to maximize coverage of host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity Purified:\u003c\/strong\u003e Affinity purification reduces non-specific background and improves assay sensitivity.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady for ELISA and Western Blot:\u003c\/strong\u003e Validated applications include HCP coverage evaluations, ELISA, and Western Blot.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eNo Preservatives:\u003c\/strong\u003e Provided in PBS without preservatives to avoid interference in downstream assays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eLong-term Stability:\u003c\/strong\u003e Short-term storage at 2-8 °C; long-term stability when stored below -65 °C (12 months validity).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluations\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot\u003c\/li\u003e\r\n                    \u003cli\u003eELISA\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Sf9 HCP Antibody (Affinity purified, unconjugated)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C short-term; long-term storage below -65 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBuffer: PBS\u003c\/span\u003e\u003cstrong\u003eAmbient to 2-8 °C (as supplied); no preservatives\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A | Method: Antibody for use in ELISA, Western Blot and HCP coverage evaluations\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Not intended for diagnostic purposes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8 °C for short-term use; for long-term storage store below -65 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Product contains no preservatives; handle under aseptic conditions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles to preserve antibody activity.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301312-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this antibody suitable for diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is for research use only and is not intended for diagnostic purposes.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What storage conditions are recommended?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMaintain at 2-8 °C for short-term use. For long-term storage, store below -65 °C. Validity period is 12 months.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What applications is the antibody validated for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe antibody is intended for HCP coverage evaluations and has documented use in Western Blot and ELISA.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Does the product contain preservatives?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The antibody is supplied in PBS and contains no preservatives.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45189119180981,"sku":"1301312-Ab01","price":4017.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-Sf9HCPAntibody.jpg?v=1742288059"},{"product_id":"hek293-hcp-elisa-kit-shentek®","title":"HEK293 HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 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#fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit); -20 °C (reconstituted standard, ≤3 freeze-thaw cycles); 2-8 °C (opened strips, up to 60 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSolid-phase, one-step double-antibody sandwich Enzyme‑Linked Immunosorbent Assay (ELISA) for quantitative determination of residual host cell proteins (HCPs) from HEK293-derived host cells. The assay uses an affinity‑purified polyclonal capture antibody coated on microtiter strips and an HRP‑conjugated anti‑HEK293 HCP polyclonal detection antibody added simultaneously with calibration standards or samples. HRP catalyzes TMB oxidation to produce a colorimetric signal (read at 450 nm with long‑wavelength correction 620–650 nm). Sample HCP concentrations are determined by fitting a 4‑parameter logistic (4‑PL) calibration curve and applying the appropriate dilution factor.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (simultaneous addition of sample\/standard and HRP conjugate)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDetermination of residual host cell proteins (HCPs) in products expressed in HEK293-derived host cells (e.g., recombinant proteins, cell and gene therapy products). For Research Use Only (RUO).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e6–540 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e6 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards\u003c\/td\u003e\r\n                        \u003ctd\u003eConcentrations: 0, 6, 18, 54, 135, 270, 540 ng\/mL (prepared from lyophilized calibration standard)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve (4‑PL)\u003c\/td\u003e\r\n                        \u003ctd\u003eA = 5.71467; B = -1.13651; C = 615.49064; D = 0.01444; R² = 0.99993\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with E. coli HCP, P. pastoris HCP, Sf9 HCP, CHO HCP and Vero HCP\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/standard + 1× Anti‑HEK293:HRP incubate on microplate thermoshaker at room temperature, 500–600 rpm for 3 hours; TMB incubation 15 min at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with correction wavelength 620–650 nm (OD450‑620 recommended)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWashing\u003c\/td\u003e\r\n                        \u003ctd\u003eWash 5 times with 340 µL of 1× Wash Buffer per well\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStop solution\u003c\/td\u003e\r\n                        \u003ctd\u003e1 M HCl (Stop Solution), read within 30 minutes after termination\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample requirements\u003c\/td\u003e\r\n                        \u003ctd\u003eSamples should be clear and transparent; remove insoluble material by centrifugation or filtration. Recommended sample pH 6.5–8.5. Avoid sodium azide in samples.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage (opened components)\u003c\/td\u003e\r\n                        \u003ctd\u003eMicrotiter strips: store in vacuumed bag with desiccant at 2–8 °C up to 60 days; Reconstituted calibration standard: store at -20 °C, limit to ≤3 freeze‑thaw cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 6 ng\/mL enables detection of low levels of residual HEK293 HCPs.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHEK293-specific detection:\u003c\/strong\u003e Affinity‑purified polyclonal antibodies raised against HEK293 HCPs provide specificity with no cross-reactivity to common host cell lines tested (E. coli, P. pastoris, Sf9, CHO, Vero).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step assay workflow:\u003c\/strong\u003e Simultaneous addition of calibration standard\/sample and HRP-conjugated detection antibody simplifies setup and reduces hands-on time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-coated strip format:\u003c\/strong\u003e Microtiter strips pre-coated with capture antibody reduce reagent preparation and ensure lot-to-lot consistency (store with desiccant).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eRobust quantitative analysis:\u003c\/strong\u003e Calibration by 4‑parameter logistic regression with demonstrated high correlation (R² ≥ 0.99).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for bioprocess QC:\u003c\/strong\u003e Applicable to in-process samples, drug substance and drug product testing for HEK293‑derived processes.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in products expressed in HEK293-derived cell lines\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing for recombinant protein production using HEK293 hosts\u003c\/li\u003e\r\n                    \u003cli\u003eAssessment of HCP clearance during downstream purification\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of in-process samples, drug substance and drug product for HEK293-derived manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eAssay development and validation during bioprocess development (method validation required)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 HCP Calibration Standard (PNB008) — lyophilized\u003c\/span\u003e\u003cstrong\u003eReconstitute with 500 µL Reconstitution Solution; reconstituted standard store at -20 °C (≤3 freeze‑thaw cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑HEK293 HCP Microtiter Strips (PNA027) — 8 wells × 12 strips\u003c\/span\u003e\u003cstrong\u003ePre-coated strips in vacuum bag with desiccant; store at 2–8 °C, reseal after use (up to 60 days for opened strips)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) — 2 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003eRoom temperature for reconstitution; store per kit instructions (2–8 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE015) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) — 1 × 25 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C; dilute 1:10 with ultrapure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑HEK293:HRP (100×) (PNN017) — 1 × 120 µL\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C (follow label); dilute 100× with Diluent immediately before use; centrifuge briefly before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003eKeep sealed, protected from light; equilibrate to room temperature 20 min before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) — 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C; avoid contact with skin\/eyes\/clothing\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 3 pieces\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (One-step double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use by qualified technicians only; validate assay before routine sample testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile disposable tips, tubes and reservoirs; avoid cross-contamination (wipe work surfaces with 75% ethanol).\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Wash Buffer by diluting 10× concentrate with ultrapure water; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge Anti‑HEK293:HRP (100×) briefly before dilution to avoid loss of reagent.\u003c\/p\u003e\r\n                    \u003cp\u003e• HEK293 HCP Calibration Standard and freshly prepared 1× Anti‑HEK293:HRP are recommended for single use due to stability concerns.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume is 5 µL; avoid excessive foaming during dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid direct contact with eyes, skin and clothing; follow appropriate safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inactivates HRP and will lead to underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not allow wells to dry before substrate addition; read plate within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store unused microtiter strips sealed with desiccant to prevent contamination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301311Z01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301311Z01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (LLOQ) and working range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay LLOQ is 6 ng\/mL with a linear range of 6–540 ng\/mL (R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store the kit and opened components?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the kit at 2–8 °C. Opened microtiter strips should be kept in the vacuum bag with desiccant at 2–8 °C (up to 60 days). Reconstituted calibration standard should be stored at -20 °C and limited to ≤3 freeze‑thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this kit detect HCPs from non‑HEK293 host cells?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis assay is specific for HEK293 HCPs and shows no cross-reactivity with E. coli, P. pastoris, Sf9, CHO or Vero HCP preparations. It is not intended for quantitation of non‑HEK293 host cell proteins.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What sample conditions or components can interfere with the assay?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSamples should be clear and free of insoluble material (centrifuge or filter as needed). Recommended pH is 6.5–8.5. Avoid sodium azide in samples as it deactivates HRP and causes underestimation of HCP levels.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical use. Users should perform method validation per applicable guidelines (e.g., USP \u0026lt;1132\u0026gt;, EP \u0026lt;2.6.34\u0026gt;, ICH Q2(R2)).\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189125669045,"sku":"1301311Z01","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HEK293_HCP_ELISA_Kit.jpg?v=1742289014"},{"product_id":"anti-hek293-hcp-antibody-shentek®","title":"Anti-HEK293 HCP Antibody SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK Anti-HEK293 HCP Antibody (Product No.: 1301311-Ab01) is an affinity-purified polyclonal sheep IgG supplied as an unconjugated liquid formulation in PBS. Immunogen: HEK293 host cell proteins (HCPs) produced by sonication cell lysis. Supplied size: 1 mg. Intended for HCP coverage evaluations and common immunodetection applications including ELISA and Western blot. No preservatives are included in the formulation. Store short-term at 2-8°C and long-term below -65°C. Validity period: 12 months.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eHuman (HEK293)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost \/ Isotype\u003c\/td\u003e\r\n                        \u003ctd\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody (Unconjugated)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd\u003eHEK293 HCPs produced by sonication cell lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd\u003eAffinity Purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage buffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage conditions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8°C for short-term use. For long-term storage, store below -65°C.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity-purified polyclonal antibody:\u003c\/strong\u003e High sensitivity and broad epitope recognition for HEK293 host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eUnconjugated format:\u003c\/strong\u003e Flexible for use with a variety of secondary reagents and detection systems (ELISA, Western blot, etc.).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated applications:\u003c\/strong\u003e Recommended for HCP coverage evaluations, Western Blot, and ELISA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDefined immunogen:\u003c\/strong\u003e Generated against HEK293 HCPs produced by sonication cell lysis to maximize coverage of host cell impurities.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluation for HEK293-derived biologics\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot detection of HEK293 host cell proteins\u003c\/li\u003e\r\n                    \u003cli\u003eELISA development and detection of host cell proteins\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-HEK293 HCP Antibody, 1 mg (unconjugated, affinity purified)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8°C for short-term use; for long-term storage keep below -65°C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStorage buffer: PBS\u003c\/span\u003e\u003cstrong\u003eAmbient (contained within vial) — follow vial storage instructions\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A | Method: Immunodetection (ELISA \/ Western Blot)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Not for diagnostic or therapeutic purposes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8°C for short-term storage. For long-term storage, store below -65°C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use if contaminated. Avoid repeated freeze-thaw cycles to preserve activity.\u003c\/p\u003e\r\n                    \u003cp\u003e• Product contains no preservatives; handle under aseptic conditions.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301311-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301311-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this antibody suitable for use in ELISA?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The antibody is supplied unconjugated and is indicated for use in ELISA. Use an appropriate secondary antibody conjugate for detection.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store the antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore short-term at 2-8°C. For long-term storage, keep below -65°C. Avoid repeated freeze-thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What was used as the immunogen and what species does it react with?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe immunogen is HEK293 host cell proteins produced by sonication cell lysis. The antibody is reactive to human HEK293 HCPs.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this product be used for diagnostic applications?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is for research use only (RUO) and is not intended for diagnostic purposes.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45189133205685,"sku":"1301311-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-HEK293HCPAntibody.jpg?v=1742289395"},{"product_id":"p-pastoris-hcp-elisa-kit-one-step-elisa-shentek®","title":"P.pastoris HCP ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA 96-well solid-phase, double-antibody sandwich one-step ELISA for quantitative detection of residual host cell proteins (HCPs) from Pichia pastoris (P. pastoris) strains (e.g., GS115, X33). The kit uses an affinity-purified sheep polyclonal anti-P.pastoris HCP capture antibody coated on microtiter strips and an HRP-conjugated sheep anti-P.pastoris HCP detection antibody added simultaneously with calibration standard or sample. TMB\/H2O2 is used as the substrate (blue product at ~655 nm; stopped to yellow product with maximum absorbance at 450 nm). Sample concentrations are calculated from a 4-parameter logistic (4-PL) dose–response calibration curve using OD450 (with 620–650 nm reference subtraction). The kit is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (pre-coated microtiter strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate format \/ Kit size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit (8-well × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinear range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2–200 ng\/mL (4-PL fit, R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve\u003c\/td\u003e\r\n                        \u003ctd\u003eStandards at 0, 2, 10, 20, 50, 100, 200 ng\/mL; example OD450-620nm average: 0 ng\/mL ~0.078; 2 ng\/mL ~0.114; 10 ng\/mL ~0.270; 20 ng\/mL ~0.434; 50 ng\/mL ~0.761; 100 ng\/mL ~1.233; 200 ng\/mL ~1.704\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eNo cross-reactivity with E. coli, CHO and Sf9 cells; \u0026lt;1% cross-reactivity with Saccharomyces cerevisiae and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with reference 620–650 nm (OD450 nm - reference)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation times\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/HRP incubation 3 hours at room temperature (600 rpm recommended); TMB substrate incubation 10 minutes; post-stop incubation\/read within recommended time\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample dilution\u003c\/td\u003e\r\n                        \u003ctd\u003eTypically ≥10-fold dilution recommended; method validation recommended to determine optimal dilutions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRead time window\u003c\/td\u003e\r\n                        \u003ctd\u003eComplete reading within 10 minutes after stopping (acceptable up to 30 minutes)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step simultaneous detection:\u003c\/strong\u003e Calibration standard (or sample) and HRP-conjugated detection antibody are added simultaneously to reduce handling steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Low limit of quantitation (LLOQ) of 2 ng\/mL across a 2–200 ng\/mL linear range with 4-PL fitting (R² ≥ 0.990).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity with common non-P.pastoris hosts (E. coli, CHO, Sf9); minimal cross-reactivity (\u0026lt;1%) with closely related yeasts.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use pre-coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with affinity-purified sheep anti-P.pastoris HCP antibody and packaged with desiccant for convenience.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Includes calibration standard, diluent, wash buffer concentrate, HRP conjugate, TMB substrate, stop solution and sealing film for full assay workflow.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in products manufactured with P. pastoris (e.g., GS115, X33)\u003c\/li\u003e\r\n                    \u003cli\u003eTesting in-process samples, harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eHCP monitoring during downstream purification and process development\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications for bioprocess development and analytical method validation\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eP.pastoris HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003e2 bottles; reconstitute with 500 µL Reconstitution Solution. Reconstituted: 2-8 °C up to 7 days or \u0026lt; -20 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-P.pastoris HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips; store in vacuumed bag with desiccant. Opened: 2-8 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e1 × 1.5 mL; room temperature equilibration for use; used to dissolve calibration standard\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; room temperature; used for dilution of standards, HRP(100×) and samples\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultra-pure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-P.pastoris :HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL HRP-conjugated affinity purified sheep antibody; centrifuge before use; dilute 100× in Diluent immediately prior to use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, keep away from light; equilibrate to room temperature (~20 min) before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; corrosive—avoid contact with eyes, skin and clothing\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C; check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate reagents to room temperature (~20 min) before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare fresh 1× Anti-P.pastoris:HRP by diluting 100× stock in Diluent immediately before use; centrifuge HRP(100×) before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard solutions and anti-P.pastoris HCP antibody solution are recommended for single use due to instability.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inactivates HRP.\u003c\/p\u003e\r\n                    \u003cp\u003e• Sample pH must be between 6.5 and 8.5; adjust or buffer samples accordingly.\u003c\/p\u003e\r\n                    \u003cp\u003e• Inactivate proteolytic enzymes in samples to prevent degradation of HCPs.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition; perform recommended wash steps (5×) and remove residual liquid.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — use appropriate PPE and avoid contact with skin\/eyes\/clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 10 minutes after stopping for most stable results (acceptable up to 30 minutes).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile, ultra-pure water; if Wash Buffer or Diluent is cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301313.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301313.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How do I reconstitute the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 500 µL Reconstitution Solution to a lyophilized calibration standard vial, invert gently and let stand ~5 minutes. Combine vials if multiple are used and mix gently. Prepare serial dilutions as described (ST1 = 200 ng\/mL, ST2 = 100 ng\/mL, etc.).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LLOQ) and linear range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLLOQ is 2 ng\/mL. The validated linear range is 2–200 ng\/mL using a 4-parameter logistic (4-PL) fit with R² ≥ 0.990.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What sample dilution should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTypically start with at least a 10-fold dilution for in-process, harvest or drug substance samples. Conduct method validation and spike-recovery experiments to determine optimal dilutions for your matrix.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store reconstituted standards and opened components?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstituted standard: 2–8 °C for up to 7 days for short-term use; for long-term storage keep below -20 °C for up to 60 days. Opened microtiter strips: store in bag with desiccant at 2–8 °C up to 60 days.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); Not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189143232693,"sku":"1301313","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P.pastoris_HCP_ELISA_Kit_One-step_ELISA.jpg?v=1742290009"}],"url":"https:\/\/biofargo.com\/collections\/residual-host-cell-protein-quantitation.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}