{"title":"Residual Host Cell DNA Size Analysis","description":"\u003cp\u003eResidual Host Cell DNA Size Analysis kits are designed to evaluate the fragment size distribution of residual DNA, a key parameter in regulatory risk assessment for biopharmaceutical products. These assays provide reliable measurement of DNA integrity and fragmentation profiles, supporting compliance with guidelines that emphasize both DNA quantity and size.\u003c\/p\u003e\n\u003cp\u003eDNA size analysis is commonly used alongside quantitative assays to deliver a more complete impurity characterization strategy.\u003c\/p\u003e","products":[{"product_id":"residual-vero-dna-size-analysis-kit-2g-shentek™","title":"Residual Vero DNA Size Analysis Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n 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solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) based assay for rapid and specific quantitation of residual Vero (host cell) DNA fragments across multiple size ranges in biopharmaceutical samples (in-process to final product). The kit is designed to amplify four discrete fragment lengths (85 bp, 134 bp, 229 bp and 552 bp) and determine size distribution and quantity down to the femtogram (fg) level. Includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, DNA dilution buffer (DDB) and a Vero DNA control for generating standard curves. Reagents are provided for 4 × 100 reactions. For sample extraction and preparation refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 134 bp, 229 bp, 552 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReal-time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects and quantifies Vero DNA down to the femtogram (fg) level (standard curve includes concentrations down to 0.03 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit dilution scheme)\u003c\/td\u003e\r\n \u003ctd\u003eST0 (prepared) 3000 pg\/µL; ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL template\/standard\/NTC (total 30 µL reaction volume). qPCR MIX composition per reaction: qPCR Reaction Buffer 15.9 µL; Vero Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL; total qPCR MIX 20 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control (IPC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eSample Ct(IPC) should be within ±1.0 Ct of NCS Ct(IPC). Significant increase indicates potential inhibition; evaluate recovery with ERC samples.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 2 Ct higher than mean Ct of the lowest standard concentration, or laboratory-specific acceptance criteria established during validation.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months; check label expiration date.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation and size-distribution analysis of residual Vero DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-fragment size analysis:\u003c\/strong\u003e Four specific primer\/probe assays amplify 85 bp, 134 bp, 229 bp and 552 bp fragments to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual DNA at femtogram-level sensitivity using absolute quantitation and serial standard curves.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBuilt-in internal control:\u003c\/strong\u003e IPC mix included to monitor inhibition and reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR reaction buffer and primer\/probe mixes minimize setup variability and speed workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus) and adjustable to other qPCR instruments following instrument software guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero (host cell) DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA fragment size distribution during downstream processing and in final product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and release testing in research and development (RUO) workflows\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery studies for host cell DNA assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-85 (NNC020) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-134 (NNC021) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-229 (NNC022) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-552 (NNC023) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProvided documentation\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions total) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read MSDS and user guide before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-decontaminate workspace, use DNase-free consumables, aliquot reagents and change tips between samples.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents as labeled; check expiration date on labels.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific settings and analysis parameters; adjust thresholds\/baselines per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: standards (ST1–ST5), NTC, NCS and IPC for each run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed for femtogram-level sensitivity. The supplied standard curve includes concentrations down to 0.03 pg\/µL (ST5). Exact LOD\/LOQ should be established during laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500 (with ROX), Bio-Rad CFX96 and LineGene 9600plus. Other real-time PCR instruments are supported; follow instrument-specific setup and dye assignments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted and what are acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSet up an absolute quantitation standard curve (ST1–ST5). IPC Ct for samples should be within ±1.0 of the NCS IPC Ct; significant deviation indicates inhibition. NTC Ct should be at least 2 Ct higher than the mean Ct of the lowest standard (or per laboratory acceptance criteria).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw Vero DNA Control and DDB on ice. Prepare ST0 at 3000 pg\/µL using DDB, then perform serial 1:10 dilutions to obtain ST1=300 pg\/µL, ST2=30 pg\/µL, ST3=3 pg\/µL, ST4=0.3 pg\/µL and ST5=0.03 pg\/µL. Include at least five standard points (suggested ST1–ST5) in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189155881141,"sku":"1103174","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Size_Analysis_Kit_2G.jpg?v=1742290678"},{"product_id":"residual-hek293-dna-size-analysis-kit-shentek™","title":"Residual HEK293 DNA Size Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HEK293 DNA Size Analysis Kit is designed to quantify residual HEK293 host cell DNA fragments of different sizes across biopharmaceutical sample types (in-process samples to final products) using real-time PCR (qPCR). The kit amplifies four fragment sizes (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer\/probe mixes for each fragment, internal positive control (IPC), DNA dilution buffer and a HEK293 DNA control for standard curve preparation. For extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eReal-time PCR (qPCR) with TaqMan-style primer \u0026amp; probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned for quantitation at the femtogram (fg) level (lowest standard in kit workflow corresponds to 0.03 pg\/µL = 30 fg)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (prepared from supplied HEK293 DNA Control)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock prior to dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear \/ working range (as provided by recommended standards)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard points from 0.03 pg\/µL to 300 pg\/µL (prepare standard curve using ST1–ST5); ST0 (3000 pg\/µL) used as stock\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR master mix + 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; HEK293 Primer\u0026amp;Probe MIX (appropriate fragment) 2.8 μL; IPC MIX 1.3 μL; total 20 μL (then add 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/read: 72 °C 01:30; 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, FQD-96A and similar real-time PCR instruments (user must configure detectors\/channels accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC \/ quality control criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. Ct of NTC and NCS should be larger than the mean Ct of the lowest concentration standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability \/ shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Simultaneous assays for four discrete fragment lengths (75, 122, 244, 562 bp) allow determination of residual HEK293 DNA size distribution.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify DNA down to the femtogram range (lowest recommended standard 0.03 pg\/µL = 30 fg).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated HEK293 Primer\u0026amp;Probe MIXes for each fragment size reduce setup time and minimize variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control (IPC):\u003c\/strong\u003e IPC MIX included to monitor inhibition and assay performance; acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible instrument compatibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (instructions include detector\/channel setup and reference dye settings).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA dilution buffer and HEK293 DNA control for standard curve preparation; refers to complementary sample prep kit for extraction.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical process and final product testing\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and process development for biologics produced in HEK293 cells\u003c\/li\u003e\r\n \u003cli\u003eRelease testing and quality control (research-use contexts)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-75 (NNC012), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-122 (NNC013), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-244 (NNC014), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-562 (NNC015), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after thaw; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control (NNA005), 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment-specific primer\/probe mix (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points (recommended ST1–ST5) and run samples in triplicate where possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR run setup: reaction volume 30 μL (20 μL master mix + 10 μL template); thermal cycling and detector settings must be configured per instrument.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: sample Ct-IPC should be within ±1.0 of NCS Ct-IPC — higher Ct-IPC indicates potential inhibition; consider recovery controls.\u003c\/p\u003e\r\n \u003cp\u003e• Negative controls (NTC) and NCS should show Ct values greater (later) than the mean Ct of the lowest standard point.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity \/ limit of detection (LOD) of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to quantify HEK293 DNA at the femtogram level. The supplied standard curve includes points down to 0.03 pg\/µL (30 fg); actual LOD\/LOQ should be established during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which fragment sizes are measured and how are they assayed?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFour fragment sizes are assayed separately using fragment-specific primer \u0026amp; probe mixes: 75 bp, 122 bp, 244 bp and 562 bp. Prepare four separate standard curves (one per fragment) and run each assay on the qPCR instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems such as SHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, and FQD-96A. Users must configure detectors\/channels, passive reference dye (e.g., ROX if required), and cycling conditions according to their instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibited?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (internal positive control). The sample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. A significantly higher Ct-IPC in the sample suggests inhibition; dilute the sample or perform recovery controls and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189170954421,"sku":"1103176","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Size_Analysis_Kit.jpg?v=1742291951"},{"product_id":"residual-pichia-pastoris-dna-quantitation-kit-shentek™","title":"Residual Pichia pastoris DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is a fluorescence-based quantitative PCR (FAM) assay designed to quantify residual Pichia pastoris host-cell DNA in biopharmaceutical materials across process stages (from in-process samples to final products). The kit includes a Pichia Pastoris DNA Control as a reference standard and provides rapid, specific quantitation with reliable sensitivity at the fg level. The kit workflow supports DNA sample dilution\/standard curve preparation, qPCR reaction mix setup and absolute quantitation using a standard curve. Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, StepOne Plus). For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Pichia pastoris DNA in biopharmaceutical samples (in-process and final products).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported quantitative range (from supplied standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 – 300 pg\/µL (equivalent to ~30 fg – 300 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReliable quantitation reported at the fg level; lowest supplied standard 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (qPCR mix 20 µL + sample 10 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003ePichia Pastoris qPCR Reaction Buffer 17 µL + Pichia Pastoris Primer\u0026amp;Probe MIX 3 µL -\u0026gt; 20 µL qPCR mix\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eProbe-based qPCR with FAM reporter dye (no quencher specified in kit instructions); use ROX as passive reference dye where applicable\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, CFX96, StepOne Plus, Linegene 9600plus, SHENTEK-96S and similar real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended. NTC Ct should be ≥ 35 cycles (or per internal validation). NCS Ct should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpike recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSpiked ERC recoveries required to be within 50% - 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ storage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stability up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003eExample workflow uses 100 µL test sample for preparation and 10 µL purified sample per qPCR reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Provides reliable quantitation at the fg level (lowest supplied standard 0.03 pg\/µL = 30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProbe-based specific detection:\u003c\/strong\u003e FAM-labeled probe assay offers high specificity for Pichia pastoris DNA fragments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard control:\u003c\/strong\u003e Includes Pichia Pastoris DNA Control for absolute quantitation and serial dilution standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated mix:\u003c\/strong\u003e Supplied reaction buffer and primer\/probe mix simplify qPCR setup and reduce preparation time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated with common real-time PCR platforms (ABI 7500, CFX96, StepOne Plus, Linegene, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow-ready for QC:\u003c\/strong\u003e Designed for in-process and final product host-cell DNA monitoring with recommended plate layouts and analysis settings.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Pichia pastoris host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and final product quality control for recombinant proteins produced in Pichia pastoris\u003c\/li\u003e\r\n \u003cli\u003eRoutine host-cell DNA monitoring during downstream purification and formulation\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike-recovery experiments for DNA residual assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris DNA Control (Part No. NNA007) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris qPCR Reaction Buffer (Part No. NNB005) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris Primer \u0026amp; Probe MIX (Part No. NNC041) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: thaw\/mix at 2–8 °C for use; unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-irradiate tabletop, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; handle primer\/probe and buffers on ice where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice and vortex to mix before use; spin briefly in microcentrifuge.\u003c\/p\u003e\r\n \u003cp\u003e• Check kit expiration date and storage conditions on labels prior to use.\u003c\/p\u003e\r\n \u003cp\u003e• Maintain recommended plate layout, replicate strategy and include NTC, NCS and ERC controls.\u003c\/p\u003e\r\n \u003cp\u003e• Spike recovery acceptance criteria: 50%–150%.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 cycles (or defined by internal validation).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit supports reliable quantitation at the fg level. The supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). Actual LOD in a given matrix should be confirmed by in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the Pichia Pastoris DNA Control and DNA Dilution Buffer (DDB) on ice. Prepare ST0 at 3000 pg\/µL then perform serial 1:10 dilutions to produce ST1–ST5: 300, 30, 3, 0.3 and 0.03 pg\/µL. Include at least five concentrations for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 95 °C for 10:00 (activation, 1 cycle), then 40 cycles of 95 °C for 00:15 (denaturation) and 60 °C for 01:00 (annealing\/extension, fluorescence read). Adjust settings if required by your instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should reagents be stored after thawing?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C per label. DNA Dilution Buffer (DDB) may be kept at 2–8 °C for short-term use; follow the instructions regarding storage of unused DDB and inspect for cloudiness\/precipitate before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171125813429,"sku":"SK030205P100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Pichia_Pastoris_DNA_Quantitation_Kit.jpg?v=1742277610"},{"product_id":"residual-cho-dna-size-analysis-kit-2g-shentek®","title":"Residual CHO DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Size Analysis Kit (2G) is a fluorescent quantitative PCR (FAM) based kit for rapid and specific quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA fragments across multiple size ranges in biopharmaceutical samples (from in-process to final products). The assay amplifies four discrete fragment sizes (95 bp, 110 bp, 215 bp and 523 bp) to produce an accurate size distribution profile. The kit includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, CHO DNA control for standard curves and DNA dilution buffer (DDB). The assay sensitivity reaches femtogram (fg) level. Refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction procedures. Product No.: 1103170-2. Reagents sufficient for 4 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e95 bp, 110 bp, 215 bp, 523 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eTarget probes: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection limit reaches femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (examples)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSuggested linear range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 0.03 – 300 pg\/µL (based on provided standard curve points)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL + CHO Primer\u0026amp;Probe MIX (per fragment) 2.8 µL + IPC MIX 1.3 µL = 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 µL (standard\/ST1–ST5, DDB for NCS, or purified sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/Read: 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selection; Threshold = 0.02; Automatic baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (kit components support ~400 reactions distributed across four fragment assays)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-size profiling:\u003c\/strong\u003e Simultaneous measurement strategy (four separate assays) for size distribution of CHO residual DNA (95, 110, 215, 523 bp) to characterize fragmentation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit at femtogram (fg) level enables quantitation of very low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated CHO Primer \u0026amp; Probe MIXes and qPCR Reaction Buffer simplify setup and reduce pipetting variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC mix included (VIC-labeled) to monitor inhibition and reaction performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600 plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA control and DNA dilution buffer for standard curves plus protocol for plate layouts and analysis.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation and size-distribution analysis of residual CHO DNA in final drug substance\/product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eRoutine QC testing of downstream purification and final product release (research and development settings)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB002)\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light (850 μL × 8 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-95 (NNC087)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-110 (NNC088)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-215 (NNC089)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-523 (NNC090)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069)\u003c\/span\u003e\u003cstrong\u003e–20 °C (550 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (NNA001)\u003c\/span\u003e\u003cstrong\u003e–20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C recommended after thaw; (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 Reactions | Method: qPCR (Fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free environment; use DNase-free consumables and low-retention tips to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% alcohol before use.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at –20 °C and check expiration dates. Kit stable up to 24 months under recommended storage.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (95, 110, 215, 523 bp). Include at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitates, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use NTC (no template control), NCS (negative control sample) and ERC (extraction reference control) as directed to monitor contamination and extraction recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Mix qPCR reactions thoroughly, seal plate, spin briefly and avoid bubble formation prior to run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit sensitivity reaches the femtogram (fg) level. Standard curve points provided include concentrations down to 0.03 pg\/µL (30 fg\/µL) and a stock at 3000 pg\/µL; actual LOD may depend on instrument and sample matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which real-time PCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Linegene 9600 plus. Other instruments supporting FAM\/VIC detection and ROX (if needed) may be used — consult instrument documentation for detector\/dye settings.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at –20 °C, protect light-sensitive reagents from light. Under recommended storage the kit is stable for up to 24 months; always verify the expiration date on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How many reactions does the kit support and how are reactions prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 4 × 100 reactions. Typical reaction setup is 20 µL qPCR MIX + 10 µL sample = 30 µL total. Prepare separate qPCR mixes for each fragment assay (95\/110\/215\/523) as described in the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175424188597,"sku":"1103170-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Size_Analysis_Kit_2G.png?v=1742290344"},{"product_id":"residual-e-coli-dna-size-analysis-kit-2g-shentek®","title":"Residual E. coli DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E.coli DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) kit for rapid and specific quantitation of residual Escherichia coli (E.coli) host-cell DNA across different fragment sizes during biopharmaceutical development and manufacturing. The kit amplifies four target fragment lengths (85 bp, 103 bp, 220 bp and 550 bp) using FAM-labeled probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. It supports absolute quantitation via user-prepared standard curves prepared from the supplied E.coli DNA Control and DNA Dilution Buffer (DDB). The kit is supplied as ready-to-use primer\/probe mixes and reaction buffer for high-throughput testing and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). For extraction\/preparation of host-cell DNA prior to qPCR, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 103 bp, 220 bp, 550 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards provided)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standard curve points prepared from E.coli DNA Control: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eImplied sensitivity \/ lowest standard\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eLowest standard = 0.03 pg\/µL (30 fg\/µL); kit described as detecting at the femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR MIX per well: 20 µL; add 10 µL sample\/standard\/NTC\/NCS for a total reaction volume of 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Instrument reads fluorescence during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescent channels \/ detectors\u003c\/td\u003e\r\n \u003ctd\u003eTargets (E.coli fragments) - FAM; IPC - VIC; Passive reference - ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eUse Absolute Quantitation (Standard Curve). Analysis settings example: Manual Ct; Threshold = 0.02; Automatic baseline. Verify slope, intercept and R2 values.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of NCS Ct-IPC. Significant increase indicates possible inhibition.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC and NCS should be larger than mean Ct of lowest standard or show no significant amplification in FAM channel while showing normal VIC signal.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components: up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 and other real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment-size analysis (four assays):\u003c\/strong\u003e Designed to quantify four discrete E.coli DNA fragment lengths (85, 103, 220 and 550 bp) to determine size distribution of residual host DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection capability down to femtogram (fg) level; lowest provided standard 0.03 pg\/µL (30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Primer \u0026amp; Probe MIX for each fragment (85, 103, 220, 550) simplifies setup and reduces handling error.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC (VIC) included to monitor inhibition and sample integrity; IPC acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility and throughput:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms and formatted for 96-well plates; reagents sufficient for 4 × 100 reactions (high-throughput).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Supplied E.coli DNA Control and dilution scheme enables generation of standard curves for absolute quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E.coli host-cell DNA in biopharmaceutical process and final products\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during process development\u003c\/li\u003e\r\n \u003cli\u003eQuality control and characterization of DNA fragment size distribution in purified biologics\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA assessment (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli DNA Control (NNA002) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-85 (NNC110) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-103 (NNC111) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-220 (NNC112) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-550 (NNC113) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (after thaw, store at 2–8 °C; if cloudy heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 400 reactions (4 × 100) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate personal protective equipment: eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use DNase-free, low-retention consumables; irradiate work surfaces and pipettes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (four assays).\u003c\/p\u003e\r\n \u003cp\u003e• qPCR reaction setup: prepare qPCR MIX (20 µL) and add 10 µL sample\/standard for 30 µL total reaction volume.\u003c\/p\u003e\r\n \u003cp\u003e• Analysis guidance: use Manual Ct, Threshold = 0.02 (example); verify slope, intercept and R2 of standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: mean Ct-IPC for sample should be within ±1.0 Ct of NCS Ct-IPC; elevated IPC Ct suggests inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC\/NCS should show no significant amplification in FAM channel or Ct greater than lowest standard; VIC signal should be present for IPC.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use to quantify residual E.coli host-cell DNA and determine fragment size distribution in biopharmaceutical samples (in-process and final products). It is RUO and not for clinical diagnostic use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instrument channels should I use for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse FAM channel(s) for E.coli target fragment detection, VIC for the internal positive control (IPC), and ROX as the passive reference according to your instrument configuration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the lowest concentration the kit can detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). The kit description indicates detection at the femtogram level; actual limit of detection should be confirmed during in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standards and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw E.coli DNA Control and DDB at 2–8 °C or on ice. Dilute DNA Control to ST0 = 3000 pg\/µL then prepare serial dilutions to produce ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentrations, 1 NTC and 1 NCS per run; run samples in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175438213301,"sku":"1103171-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Size_Analysis_Kit_2G.png?v=1742290413"},{"product_id":"residual-mdck-dna-size-analysis-kit-shentek®","title":"Residual MDCK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Size Analysis Kit is designed to quantify fragment sizes of residual MDCK host cell DNA in biopharmaceutical samples across process stages (in‑process to final products). The kit utilizes a real‑time PCR (qPCR) approach to rapidly and specifically quantify MDCK DNA fragments using FAM‑labeled probes. It amplifies four discrete fragment lengths (84 bp, 142 bp, 204 bp and 504 bp) to determine size distribution with a limit of detection in the femtogram (fg) range. An internal positive control (IPC, VIC) is included to monitor inhibition and assay performance. For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). Reagents supplied support 4 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e84 bp, 142 bp, 204 bp, 504 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003efemtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume and template input\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; MDCK Primer \u0026amp; Probe MIX 2.8 μL (per target); IPC MIX 1.3 μL; total 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions (example \/ ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30 (read fluorescence); 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC. If significantly higher, sample may be inhibitory. NTC Ct should be ≥ 35 or undetermined.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions total)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFour discrete fragment assays:\u003c\/strong\u003e Simultaneous\/parallel assays for 84 bp, 142 bp, 204 bp and 504 bp fragments for size distribution profiling.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection at the femtogram level for detection of low‑abundance residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR based, rapid and specific:\u003c\/strong\u003e Real‑time PCR with FAM probe detection provides rapid and specific quantitation of MDCK residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal inhibition control (IPC):\u003c\/strong\u003e VIC‑labeled IPC included to monitor sample inhibition and assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use primer\/probe mixes:\u003c\/strong\u003e Preformulated MDCK Primer \u0026amp; Probe MIXes for each fragment length reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for SHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution for process development and validation\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for residual host cell DNA in vaccine and biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and sample inhibition assessment using IPC\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑84 (NNC025) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑142 (NNC026) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑204 (NNC027) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑504 (NNC028) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (not for diagnostic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (84, 142, 204, 504 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five concentrations for the standard curve and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC; if higher, sample may be inhibitory — consider sample recovery testing.\u003c\/p\u003e\r\n \u003cp\u003e• NTC criterion: Ct for NTC should be ≥ 35 cycles or undetermined.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and spin samples before running.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components as labeled and check expiration dates before use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103175.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103175.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit contains primer\/probe mixes for four fragment sizes: 84 bp, 142 bp, 204 bp and 504 bp.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL qPCR MIX + 10 μL template\/sample). Prepare qPCR MIX according to the protocol tables for each fragment assay.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How sensitive is the assay and what standards should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has a limit of detection at the femtogram level. Prepare the supplied MDCK DNA Control serial dilutions (examples: ST0 3000 pg\/μL down to ST6 0.003 pg\/μL) and include at least five standard concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibitory?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eMonitor the IPC (VIC) signal. The mean Ct‑IPC for a sample should be within ±1.0 of the NCS Ct‑IPC. A significantly higher Ct‑IPC indicates potential inhibition and you should evaluate sample recovery or dilution.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175564304565,"sku":"1103175","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Size_Analysis_Kit.png?v=1742291658"},{"product_id":"residual-sf9-residual-dna-size-analysis-kit-shentek®","title":"Residual Sf9 Residual DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9 DNA Size Analysis Kit is a qPCR-based kit for rapid, specific quantitation and size distribution analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (from in‑process to final products). The assay amplifies four target fragment lengths (87 bp, 118 bp, 225 bp and 500 bp) using FAM-labeled detection and includes an internal positive control (IPC, VIC). The kit achieves sensitivity at the level of femtograms (fg). This package contains reagents to perform 4 × 100 reactions (primer\/probe mixes for four fragment sizes, qPCR reaction buffer, Sf9 DNA control, IPC mix and DNA dilution buffer). For DNA extraction\/sample preparation refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e87 bp, 118 bp, 225 bp, 500 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity at femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (recommended)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, ST6 = 0.003 pg\/µL (Note: MIX-87 and MIX-118: ST1–ST6; MIX-225 and MIX-500: ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per well = 20 µL; template\/sample = 10 µL; total reaction volume = 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9 Primer\u0026amp;Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling profile\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles (instrument reads fluorescence during extension step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt and analysis thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold = 0.02 (FAM); VIC (IPC) threshold = 0.1; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification; sample Ct-IPC should be within ±1.0 Ct of NCS Ct-IPC (IPC used to detect inhibition)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eRecommend triplicates for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility \/ instruments\u003c\/td\u003e\r\n \u003ctd\u003eValidated on SHENTEK-96S, ABI 7500, and LightCycler 480 II; compatible with other real-time PCR systems (follow instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable for up to 24 months when stored as indicated on labels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative size analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (research applications)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFour-size multiplexed analysis (separate assays):\u003c\/strong\u003e Designed to determine size distribution by amplifying four discrete fragment lengths (87, 118, 225, 500 bp) for accurate fragment-size profiling.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LOD at the femtogram level enables detection and quantitation of trace residual Sf9 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Standard-curve (absolute quantitation) workflow with serial dilutions and recommended concentration points for accurate quantification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC (VIC channel) included to monitor reaction integrity and identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Sf9 Primer \u0026amp; Probe MIXes for each fragment length reduce setup variability and save time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, LightCycler 480 II); protocol includes instrument-specific guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eResidual DNA analysis in final biologic drug products derived from Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eSize distribution profiling of host-cell DNA fragments (87–500 bp range)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC for host DNA clearance processes (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 DNA Control (NNA033), 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB002), 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-87 (NNC060), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-118 (NNC061), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-225 (NNC062), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-500 (NNC063), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB store at 2–8 °C after thawing)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surface, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at temperatures indicated on labels; kit stable up to 24 months.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear; store unused DDB at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length; include at least five standard points.\u003c\/p\u003e\r\n \u003cp\u003e• Run technical replicates (triplicates recommended) and include NTC and NCS controls.\u003c\/p\u003e\r\n \u003cp\u003e• Interpret IPC Ct: sample mean Ct-IPC should be within ±1.0 of NCS Ct-IPC; higher IPC Ct indicates inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103177.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103177.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume and reaction setup should I use for each well?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 20 µL qPCR MIX plus 10 µL of template (purified sample, NCS or standard) for a total reaction volume of 30 µL per well.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which standard concentrations are recommended for the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse the recommended standard series: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, and ST6 = 0.003 pg\/µL. Note: use ST1–ST6 for MIX-87 and MIX-118; use ST1–ST5 for MIX-225 and MIX-500.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I know if my sample contains PCR inhibitors?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (VIC) Ct value: the sample mean Ct-IPC should be within ±1.0 Ct of the NCS Ct-IPC. A significantly higher IPC Ct in the sample indicates possible inhibition; consider dilution or additional purification and retest.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15; Annealing 60 °C for 00:30; Extension 72 °C for 01:30; 40 cycles. Instrument reads fluorescence during the extension step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175577018549,"sku":"1103177","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_Residual_DNA_Size_Analysis_Kit.png?v=1742292573"},{"product_id":"residual-human-dna-size-analysis-kit-2g-shentek®","title":"Residual Human DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Human DNA Size Analysis Kit (2G) is designed for the rapid and specific quantitation of residual human DNA fragments of different sizes in biopharmaceutical samples (from in‑process to final products) using real‑time PCR (qPCR). The assay amplifies four distinct fragment lengths (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer \u0026amp; probe mixes for each fragment, an internal positive control (IPC), qPCR Reaction Buffer, DNA dilution buffer and a Human DNA Control for standard curve generation. For DNA extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). This product is intended for Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFragment lengths amplified\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; standard curve down to 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (mix + template)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR mix per reaction = 20 µL (15.9 µL qPCR Reaction Buffer + 2.8 µL Primer\u0026amp;Probe Mix + 1.3 µL IPC MIX) + 10 µL template = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold guidance\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-75 = 0.04; Human-122\/244\/562 = 0.02. IPC Ct of sample should be within ±1.0 of NCS.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable up to 24 months (check label expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A and other real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment analysis:\u003c\/strong\u003e Assays four discrete human DNA fragment sizes (75, 122, 244 and 562 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram range with standard curve points to 0.03 pg\/µL.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment and IPC MIX to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC-focused workflow:\u003c\/strong\u003e Includes Human DNA Control for standard curves, NTC and NCS recommendations, and IPC monitoring for inhibition\/recovery.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms and configurable for different software\/instruments.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human DNA in biopharmaceutical products (in-process to final product)\u003c\/li\u003e\r\n \u003cli\u003eHost cell \/ residual DNA monitoring for process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing workflows for biologics\u003c\/li\u003e\r\n \u003cli\u003eComparative fragment size distribution analysis of residual DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-75 (NNC008) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-122 (NNC009) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-244 (NNC010) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-562 (NNC011) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL ×3\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points, NTC and NCS; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC Ct of sample should be within ±1.0 of NCS; significantly higher IPC Ct suggests assay inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC and NCS Ct values should be larger than mean Ct of the lowest standard concentration in the FAM channel.\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters and thresholds according to instrument model and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103173.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103173.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the kit and reagents?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore primer\/probe mixes, IPC MIX, Human DNA Control and qPCR Reaction Buffer at -20 °C. Store DNA Dilution Buffer (DDB) at 2–8 °C. The kit components are stable up to 24 months; verify expiration dates on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve should I use for quantitation?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 5‑point standard curve for each fragment using the provided Human DNA Control serial dilutions: 300, 30, 3, 0.3 and 0.03 pg\/µL (ST1–ST5). Include NTC and NCS controls and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions and reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR mix + 10 µL template). Thermal cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30 with fluorescence read during extension.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the IPC Ct indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf IPC Ct for a sample is significantly higher than the NCS (outside ±1.0), suspect inhibition. Consider re‑extracting or diluting the sample, check sample recovery (if ERC included), and reassess prior to reporting.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175607951541,"sku":"1103173","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Size_Analysis_Kit_2G.jpg?v=1742292680"},{"product_id":"residual-bhk-dna-size-analysis-kit-shentek®","title":"Residual BHK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Size Analysis Kit is designed for quantitative measurement of residual BHK host-cell DNA fragments across different fragment sizes in biopharmaceutical samples (from in-process samples to final products). The assay uses quantitative PCR with FAM-labeled probes to amplify four discrete fragment sizes (81 bp, 134 bp, 216 bp and 589 bp) to determine size distribution and quantity. The kit provides high sensitivity with a reported limit of detection (LOD) at the femtogram (fg) level. An internal positive control (IPC, VIC channel) is included to monitor inhibition and extraction\/reaction performance. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e81 bp, 134 bp, 216 bp, 589 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFemtogram (fg) level sensitivity (LOD stated as fg level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit instructions)\u003c\/td\u003e\r\n \u003ctd\u003eStandards used (ST1-ST5): 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (serial dilutions prepared from ST0 source)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR reaction volume: 30 µL total (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; BHK Primer \u0026amp; Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Fluorescence is read during the extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd\u003eTarget probes: FAM reporter for each BHK fragment assay; IPC: VIC reporter. ROX recommended as passive reference (ABI instruments).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles; NCS Ct should be greater than mean Ct of the lowest standard and show normal VIC amplification. IPC Ct of sample should be within ±1.0 of NCS IPC Ct; deviations indicate inhibition.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSample recovery rate (ERC-based) should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (stated in product label \/ packaging)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantitation (LOQ) not numerically specified in the user guide; users should refer to assay validation for LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti‑size quantitation:\u003c\/strong\u003e Simultaneous assessment of residual BHK DNA across four discrete fragment sizes (81, 134, 216, 589 bp) to provide size distribution information.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity reaches femtogram (fg) level LOD for detection of low-abundance residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based specificity:\u003c\/strong\u003e TaqMan-style primer\/probe assays (FAM) deliver rapid and specific quantitation; IPC (VIC) monitors inhibition and reaction integrity.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe MIXes simplify assay setup and reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (examples: SHENTEK-96S, ABI 7500, Roche LightCycler 480) and configurable for other real-time PCR systems.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eSize-distribution analysis of residual DNA in in-process and final drug product samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing during upstream\/downstream processing and release testing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-81 (NNC072) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-134 (NNC073) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-216 (NNC074) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-589 (NNC075) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 total reactions, as stated on product label) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (see compliance). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Avoid nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C (on ice), vortex and briefly centrifuge before use; protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curves (minimum five concentrations recommended) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR reaction setup: 20 µL qPCR MIX + 10 µL sample\/standard = 30 µL total per well; seal plates and centrifuge briefly prior to run.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35; IPC Ct of sample should be within ±1.0 of NCS IPC Ct; recovery rates should be between 50% and 150%.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear and store remaining DDB at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Limit of quantitation (LOQ) must be established\/validated by user; if LOQ is lower than lowest standard, NCS Ct should be below LOQ concentration.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103179.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103179.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect and quantify?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit amplifies and quantifies four discrete BHK host‑cell DNA fragment sizes: 81 bp, 134 bp, 216 bp and 589 bp.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports a limit of detection (LOD) at the femtogram (fg) level. Exact LOD\/LOQ values should be confirmed during in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What reaction volume and thermal cycling program should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30. Fluorescence is read during the extension step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC, NTC and NCS results?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles. IPC Ct of a sample should be within ±1.0 of the NCS IPC Ct; a higher IPC Ct indicates potential inhibition. NCS Ct should be greater than the mean Ct of the lowest standard and exhibit normal VIC amplification. Recovery rate (ERC-based) should be 50–150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175635017909,"sku":"1103179","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Size_Analysis_Kit.jpg?v=1742292816"},{"product_id":"residual-pg13-dna-size-analysis-kit-shentek®","title":"Residual PG13 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual PG13 DNA Size Analysis Kit is designed to quantify residual PG13 host-cell DNA across different fragment sizes during biopharmaceutical production (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (FAM) assay to rapidly and specifically measure PG13 DNA fragments. The assay amplifies three discrete target fragment sizes (118 bp, 212 bp and 502 bp) to determine size distribution. The kit includes an internal positive control (IPC) and a PG13 DNA control for generation of absolute-quantitation standard curves. Limit of detection (LOD) reaches the femtogram (fg) level. For DNA extraction recommendations, refer to the companion SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e118 bp, 212 bp, 502 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eLOD reaches femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per 1 reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; PG13 Primer\u0026amp;Probe MIX (per target) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30 (read fluorescence) — 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument\/readout channels\u003c\/td\u003e\r\n \u003ctd\u003eFAM for PG13 targets; VIC for IPC; ROX used as passive reference (ABI7500 example)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) for sample should be within ±1.0 of NCS Ct(IPC); a significantly higher Ct indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery rate (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExpected ERC recovery between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC and NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n \u003ctd\u003eComponents stored at –20 °C; stable for up to 24 months (check label expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reagent format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 3 × 100 reactions (stated on product cover)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Three separate primer\/probe assays (118 bp, 212 bp, 502 bp) enable determination of residual DNA size distribution, not just total DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay LOD reaches femtogram (fg) levels for detection of trace residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA control and recommended serial dilution scheme to build standard curves for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition monitoring:\u003c\/strong\u003e IPC (VIC) included to monitor PCR inhibition and assay performance on a per-sample basis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe mixes reduce setup time and variability.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA fragmentation during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product quality control and release testing for residual host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for gene therapy\/viral vector production using PG13-derived material\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 µL × 6 tubes (protect from light)\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-118 (NNC083) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-212 (NNC084) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-502 (NNC085) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) — 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 3 × 100 reactions (300 reactions) | Method: qPCR (fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Work in a clean, DNase-free environment; decontaminate surfaces and equipment (UV 30 minutes and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and brief-spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store components at –20 °C; check expiration dates on labels.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitate, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: at least 5 standard points, NTC, and NCS; recommended technical triplicates for samples.\u003c\/p\u003e\r\n \u003cp\u003e• IPC performance: sample mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant deviation may indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction recovery control) recovery should be 50%–150%; investigate out-of-range recoveries.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥35 cycles or undetermined; NCS should be greater than lowest standard Ct and show VIC amplification.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103178.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103178.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms, including SHENTEK-96S, ABI 7500 series, and LightCycler 480. If using a different instrument, follow the instrument\/software documentation for detector and plate setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw PG13 DNA Control and DDB at 2–8 °C. Dilute the DNA Control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five standards in the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are acceptable IPC and recovery criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct(IPC) for a sample should be within ±1.0 of the NCS Ct(IPC). ERC recovery rates should fall between 50% and 150%; values outside this range warrant investigation for extraction efficiency or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if my DNA Dilution Buffer is cloudy?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf DDB is cloudy or contains precipitate, heat the buffer at 37 °C until it becomes clear, then cool and use. Store DDB at 2–8 °C after opening or per kit instructions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"3×100 Reactions","offer_id":45175694983349,"sku":"1103178","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Size_Analysis_Kit.jpg?v=1742292887"},{"product_id":"residual-hpv18-e6-e7-dna-size-analysis-kit-shentek®","title":"Residual HPV18 E6\/E7 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eApproximately 1 hour\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HPV18 E6\/E7 DNA Size Analysis Kit is a real-time PCR (qPCR) kit designed for rapid and specific quantitation of residual HPV18 E6 and E7 DNA fragments of different sizes (from HeLa cell origin) across biopharmaceutical sample types (in-process to final products). The kit amplifies four fragment lengths (E6: 100 bp and 288 bp; E7: 110 bp and 240 bp) using FAM-labeled target probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. The kit includes HPV E6\/E7 DNA Control for standard curve generation and is intended for research use only. For sample extraction instructions refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget genes \/ fragments\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eHPV18 E6 (100 bp, 288 bp) and E7 (110 bp, 240 bp)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ channels\u003c\/td\u003e\r\n \u003ctd\u003eFAM-labeled probes for target fragments; VIC-labeled probe for IPC; ROX as passive reference (recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample or standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + primer\u0026amp;probe mix 3 μL (per target assay); IPC qPCR mix: qPCR Reaction Buffer 15.9 μL, IPC MIX 1.3 μL, ddH2O 2.8 μL (per 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during annealing\/extension)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ dynamic range (provided example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard curve points shown: 1×10^8 to 1×10^2 copies\/μL (ST0 = 1×10^8, ST1 = 1×10^7 ... ST6 = 1×10^2); at least five concentrations recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicates recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls included \/ required\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eHPV E6\/E7 DNA Control (quantified) for standard curve; NTC (no template control); NCS (negative control sample); IPC for inhibition monitoring\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInput\/sample volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample or 10 μL standard per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (as labeled on product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eUp to 24 months when stored at –20 °C (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNot specified in the user guide; recommended to determine during in-house validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInhibition acceptance criterion\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMean Ct of IPC for sample should be within ±1.0 of the NCS Ct-IPC; significant increase suggests inhibition and sample recovery testing is recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus and other real-time PCR systems (user must configure detectors\/filters accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFragment-size specific quantitation:\u003c\/strong\u003e Designed to amplify and quantify four distinct HPV18 E6\/E7 fragment sizes (E6:100 bp \u0026amp; 288 bp; E7:110 bp \u0026amp; 240 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReal-time qPCR with internal control:\u003c\/strong\u003e Uses FAM-labeled probes for targets and VIC-labeled IPC to monitor inhibition and assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated primer \u0026amp; probe mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment simplify setup and reduce pipetting errors; probes are light-sensitive (protect from light).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use standards:\u003c\/strong\u003e Includes quantified HPV E6\/E7 DNA Control and detailed serial dilution protocol for building standard curves and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Provides recommended plate layouts, qPCR conditions, and data analysis settings for ABI7500 (can be adapted to other instruments).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HPV18 E6\/E7 DNA (HeLa origin) in biopharmaceutical production\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and final product quality control for residual host cell DNA (HPV18-specific)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual DNA size-distribution analysis\u003c\/li\u003e\r\n \u003cli\u003eSample inhibition assessment and recovery evaluation using IPC\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX\u003c\/span\u003e\u003cstrong\u003e150 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE6-100 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE6-288 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE7-110 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE7-240 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e850 μL × multiple tubes (as supplied); –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB, NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL ×2 tubes; store at 2–8 °C (if cloudy, heat to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHPV E6\/E7 DNA Control (NNA030)\u003c\/span\u003e\u003cstrong\u003e50 μL ×1 tube; –20 °C (check label for concentration before dilution)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR (real-time PCR, absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mixes and IPC MIX from light; store kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes (UV 30 minutes recommended) and use dedicated pre- and post-PCR areas to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run NTC, NCS and IPC controls as instructed. NCS must be processed the same as test samples.\u003c\/p\u003e\r\n \u003cp\u003e• If IPC Ct for a sample is \u0026gt; ±1.0 compared to NCS IPC Ct, the sample may contain inhibitors; perform recovery testing or further sample cleanup.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific software settings and thresholds; example settings provided for ABI 7500 (Manual Ct, threshold 0.02, automatic baseline).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the expected assay runtime?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe qPCR program provided runs activation 95 °C for 10 min plus 40 cycles of 95 °C for 15 s and 60 °C for 60 s, resulting in an approximate total runtime of ~1 hour per run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the supplied HPV E6\/E7 DNA Control, dilute to prepare ST0 at 1×10^8 copies\/μL, then perform serial 1:10 dilutions to generate ST1–ST6 (1×10^7 to 1×10^2 copies\/μL). Include at least five concentrations across the dynamic range and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if the IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample mean Ct-IPC is significantly higher than NCS Ct-IPC (\u0026gt;±1.0), the sample may be inhibitory. Perform recovery testing (ERC) in the same assay, consider additional sample cleanup or dilution, and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What is the assay sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eLOD\/LOQ are not specified in the user guide. Determine LOD\/LOQ, precision and accuracy during in-house validation using the provided standard curve and appropriate validation procedures.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Products are intended for research use only.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175697965237,"sku":"SK030307S-P","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HPV18_E6_E7_DNA_Size_Analysis_Kit.jpg?v=1742292960"}],"url":"https:\/\/biofargo.com\/collections\/residual-host-cell-dna-size-analysis.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}