{"title":"Residual Host Cell DNA Quantitation","description":"\u003cp\u003eOur Residual Host Cell DNA Quantitation kits enable highly sensitive detection and quantification of trace host cell DNA using qPCR-based and fluorescence-based methodologies. These assays are optimized for low background, broad dynamic range, and high reproducibility, making them suitable for monitoring DNA clearance during downstream purification and final product testing.\u003c\/p\u003e\n\u003cp\u003eCommon applications include CHO-derived products, Vero cell–based vaccines, HEK293 expression systems, and other mammalian or microbial production platforms.\u003c\/p\u003e","products":[{"product_id":"residual-e-coli-dna-quantitation-kit-shentek®","title":"Residual E. coli DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n 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solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E. coli DNA Quantitation Kit is designed for quantitative measurement of residual E. coli host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (qPCR) to enable rapid and specific quantitation at the femtogram level. It is suitable for a broad range of sample types from in-process samples to final products. The kit includes an E. coli Control DNA traceable to a primary DNA reference standard and a ready-to-use qPCR mix. For nucleic acid extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as supplied with the kit).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (absolute quantitation using standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (standard curve)\u003c\/td\u003e\r\n \u003ctd\u003eStandard curve concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03 pg\/µL (recommended at least five concentration points).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eReported sensitivity: femtogram level (kit statement). Practical lowest standard used: 0.03 pg\/µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eqPCR reaction volume set to 30 µL (instrument setting described uses 30 µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/extension: 60 °C 01:00 (read fluorescence during this step).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended (ABI7500 SDS v1.4 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative and control acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NCS Ct should be larger than mean Ct of the lowest standard; NTC should be undetermined or Ct ≥ 35.00.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component labels).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus, Mx3000P, qTOWER3G, etc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Enables quantitation at the femtogram level; lowest standard shown at 0.03 pg\/µL.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTraceable control:\u003c\/strong\u003e Includes E. coli Control DNA traceable to a primary DNA reference standard for reliable standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Supplied E. coli qPCR MIX is provided in ready-to-use format to simplify reaction setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Validated conceptually for a variety of biopharmaceutical sample types from in-process to final products (recommended to perform method validation for specific matrices).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Protocol and plate layouts provided for a range of common real-time PCR instruments.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of residual host DNA during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing (research use applications)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli qPCR MIX (NNC002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1 mL × 2 tubes (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using a standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use RNase\/DNase-free consumables, low-retention filter tips, UV-decontaminate surfaces and pipettes, and use separate areas for pre- and post-amplification steps.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents as indicated.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations for a reliable standard curve and perform method validation for your sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery range: 50%–150%; verify NTC is undetermined or Ct ≥ 35 and NCS Ct is larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific software and parameter settings for data analysis (example settings provided for ABI 7500 SDS v1.4).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research quantitation of residual E. coli host cell DNA in biopharmaceutical samples using qPCR (Research Use Only).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What are the standard curve concentrations supplied in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes preparing standards at 3000, 300, 30, 3, 0.3 and 0.03 pg\/µL (use at least five concentrations for the standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%; NTC should be undetermined or Ct ≥ 35; NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I set up the qPCR cycling program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended example: 95 °C for 10:00 (activation, 1 cycle); 95 °C for 0:15 (denaturation) and 60 °C for 1:00 (annealing\/extension with fluorescence read) for 40 cycles. Reaction volume example: 30 µL. Adjust program per instrument requirements.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186991784117,"sku":"SK030202E100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit.jpg?v=1742269328"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-e-coli-dna-quantitation-kit-2g-shentek™","title":"Residual E. coli DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli DNA Quantitation Kit (2G) is a duplex real‑time PCR (qPCR) assay designed to quantitate residual Escherichia coli host cell DNA in biopharmaceutical samples from in‑process material to final products. The kit provides high sensitivity (fg level), rapid and specific absolute quantitation using an internal positive control (IPC) included in the Primer \u0026amp; Probe mix to monitor PCR performance. The kit includes DNA control, qPCR master mix, DNA dilution buffer and Primer \u0026amp; Probe mix (with IPC). For extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Supplied reagents are sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (in‑process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real‑time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReported sensitivity at the fg level (document states fg level performance)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared serial dilutions: ST0 3000 pg\/µL (stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume (program)\u003c\/td\u003e\r\n \u003ctd\u003eThermal cycling reaction volume set to 30 µL (instrument setting referenced)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15, Annealing\/Extension 60 °C 1:00, 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt thresholds \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct used. Thresholds: E.coli‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC sample recovery: 50%–150%. NTC Ct ≥ 35.00. NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. IPC monitored in VIC channel.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under specified conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, LightCycler 480 (and other compatible real‑time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real‑time PCR:\u003c\/strong\u003e Simultaneous detection of E. coli target and IPC in the same reaction for reliable quantitation and internal reaction control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual E. coli DNA down to the fg level using an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use components:\u003c\/strong\u003e Includes DNA control, qPCR Master Mix, Primer \u0026amp; Probe mix (with IPC) and DNA Dilution Buffer for streamlined workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Built‑in criteria for NTC, NCS and ERC recovery (50%–150%) to support routine QC and method validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument support:\u003c\/strong\u003e Validated for common real‑time PCR instruments (ABI 7500, CFX96, LightCycler 480, SHENTEK‑96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical raw materials, intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and in‑process monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of biologics for host cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and CRO testing workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli Primer \u0026amp; Probe MIX (incl. IPC) (NNC115) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDSs) before use; wear appropriate protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNA‑free consumables; perform UV decontamination of tabletop, pipettes and tubes for 30 minutes and disinfect surfaces with 75% ethanol to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and spin briefly. Protect light‑sensitive reagents (Master MIX and Primer \u0026amp; Probe MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C. Unused DNA Dilution Buffer (DDB) may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (standard curve, NTC, NCS, ERC) and run samples in replicates (recommended triplicates).\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument‑specific settings when adapting the qPCR program; provided cycling parameters and analysis thresholds are validated on ABI 7500 SDS v1.4 and may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity \/ limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports assay performance at the fg level (ultra‑sensitive); exact numeric LOD is not specified in the document and should be determined during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the supplied E. coli DNA Control to generate at least five standard points. Example dilutions in the guide: ST1 300 pg\/µL, ST2 30 pg\/µL, ST3 3 pg\/µL, ST4 0.3 pg\/µL, ST5 0.03 pg\/µL. Include standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC Ct should be ≥ 35.00. NCS Ct should be greater than the mean Ct of the lowest standard and show normal amplification in the VIC (IPC) channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186998108341,"sku":"1101107-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit_2G.jpg?v=1742277494"},{"product_id":"residual-cho-dna-quantitation-kit-2g-shentek™","title":"Residual CHO DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit (2G) is intended to quantitate host-cell line residual DNA from CHO cell lines used in biopharmaceutical production. The kit uses duplex real-time PCR (qPCR) technology to perform rapid, specific and reliable quantitation down to the fg level. An Internal Positive Control (IPC) is included in the CHO Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit provides ready-to-use qPCR Master Mix, CHO Primer \u0026amp; Probe MIX with IPC, a quantified CHO DNA Control for standard curve generation, and DNA Dilution Buffer. For DNA extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Product No.: 1101100-1. Reagents for 100 reactions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (label on kit: Reagents for 100 Reactions).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (serial dilutions)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL (stock), ST1 300 pg\/μL, ST2 30 pg\/μL, ST3 3 pg\/μL, ST4 0.3 pg\/μL, ST5 0.03 pg\/μL, ST6 0.003 pg\/μL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e3000 pg\/μL to 0.003 pg\/μL (nominal dynamic range as provided by standard series).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD (document statement)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims quantitation at the fg level; lowest provided standard is 0.003 pg\/μL (3 fg\/μL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX (15 μL qPCR Master MIX + 5 μL CHO Primer\u0026amp;Probe MIX) + 10 μL sample\/standard\/NTC\/NCS = 30 μL final reaction volume.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extension, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels and dyes\u003c\/td\u003e\r\n \u003ctd\u003eCHO-DNA detector: FAM reporter; IPC detector: VIC reporter; Passive reference: ROX.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected. Threshold: CHO-DNA = 0.05, IPC = 0.1. Automatic baseline recommended. Instrument-specific settings may vary.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptability criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35.00 (or laboratory-specific threshold). NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. ERC recovery should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit components storage conditions\u003c\/td\u003e\r\n \u003ctd\u003eAll listed components stored at -20 °C; protect light where indicated. Kit stable up to 24 months (check label expiration).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ version \/ manufacturer\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1101100-1. User Guide Version: A\/1. Effective date: 01 Jul. 2024. Manufacturer: Biofargo, Inc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR (qPCR):\u003c\/strong\u003e Simultaneous detection of CHO target sequence and an internal positive control (IPC) in the same reaction to monitor assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at the femtogram level; includes a dilution series down to 0.003 pg\/μL (3 fg\/μL) standard.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and CHO Primer \u0026amp; Probe MIX (with IPC) minimize assay setup time and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for common real-time PCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Roche LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes quantified CHO DNA Control for standard curve generation, DNA Dilution Buffer (DDB), and IPC in the primer\/probe mix.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceuticals\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and process development for biologics produced in CHO cells (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control assays in research settings where CHO DNA detection is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer \u0026amp; Probe MIX (including IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five standard curve points; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct acceptance: ≥ 35.00 (or lab-specific threshold). NCS Ct should be greater than mean Ct of lowest standard and display normal VIC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction reference control) recovery must be between 50% and 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation at the femtogram level. The lowest supplied standard is 0.003 pg\/μL (3 fg\/μL); actual limit of detection should be confirmed by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction (15 μL qPCR Master MIX + 5 μL CHO Primer \u0026amp; Probe MIX) and add 10 μL sample or standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which fluorescence channels are used for target and control?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCHO target is detected in the FAM channel; the internal positive control (IPC) is detected in the VIC channel; ROX is used as passive reference when applicable.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35.00 (or a lab-defined threshold). NCS Ct should be higher than the mean Ct of the lowest standard and show VIC amplification. ERC recovery should fall between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187351445685,"sku":"1101100-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit_2G.jpg?v=1742278220"},{"product_id":"residual-hek293-dna-quantitation-kit-3g-shentek™","title":"Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual HEK293 DNA Quantitation Kit (3G) is designed to quantify residual HEK293 host cell DNA in biopharmaceutical samples at various stages (in‑process to final product). The kit uses duplex real‑time PCR (qPCR) for rapid, specific and reliable quantitation at the fg level. An Internal Positive Control (IPC) is included in the HEK293 Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit supports extraction workflows (refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit, Product No. 1104191) and is supplied as ready‑to‑use master mix and primer\/probe reagents for up to 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDuplex real‑time PCR (qPCR) with target (FAM) and IPC (VIC) channels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX + 10 μL sample\/control = 30 μL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD guidance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit claims quantitation at the fg level. Lowest standard: 0.03 pg\/μL (30 fg\/μL). NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific limit based on validation).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery \/ Accuracy guidance\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (example for ABI7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct. Thresholds: HEK293‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, BioRad CFX96, Roche LightCycler 480 and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims quantitation at the femtogram (fg) level with standard curve down to 0.03 pg\/μL (30 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (IPC) in the Primer \u0026amp; Probe MIX to monitor inhibition and reaction performance in each well.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use reagents:\u003c\/strong\u003e Supplied as qPCR Master MIX and premixed Primer \u0026amp; Probe, minimizing setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated on multiple real‑time PCR platforms (e.g., SHENTEK‑96S, ABI7500, CFX96, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharmaceutical testing:\u003c\/strong\u003e Optimized for residual host cell DNA measurement across in‑process and final product samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in upstream and downstream bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn‑process and final product residual DNA testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer \u0026amp; Probe MIX (Includes IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, lab coat, protective eyewear, mask) and work in nuclease‑free conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and consumables by UV irradiation (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge. Protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Perform laboratory‑specific validation to establish LOD, LOQ and acceptance criteria.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure plate layout includes appropriate standards (at least five concentrations), NTC, NCS and ERC samples; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the claimed sensitivity of the kit and what are the lowest standard concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit claims quantitation at the femtogram (fg) level. The provided standard curve points include ST1 = 300 pg\/μL down to ST5 = 0.03 pg\/μL (30 fg\/μL). Laboratories should perform validation to determine actual LOD\/LOQ in their workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I set up reaction volumes and plate layout?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction and add 10 μL of sample\/control to each well for a total reaction volume of 30 μL. Include at least five standard points, one NTC, one NCS and ERCs as described; triplicate wells are recommended for each sample.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended cycling conditions and analysis settings?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample conditions (ABI7500 SDS v1.4): Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 1:00 with fluorescence read during the 60 °C step. Suggested thresholds: HEK293‑DNA = 0.05; IPC = 0.1; use manual Ct and automatic baseline or adapt per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should I use for ERC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific standard based on validation). The Ct of NCS should be larger than the mean Ct of the lowest standard and show IPC amplification in the VIC channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189158633653,"sku":"1101104-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Quantitation_Kit_3G.jpg?v=1742279848"},{"product_id":"residual-plasmid-dna-quantitation-kit-3g-shentek™","title":"Residual Plasmid DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Plasmid DNA Quantitation Kit (3G) is a duplex real-time PCR (qPCR) kit designed to quantify residual plasmid DNA in gene therapy products (for example, plasmid DNA in lentiviral vector preparations used in CAR‑T manufacturing). The assay targets consensual plasmid sequences (replicon sequences of ColE1\/pMB1\/pBR322\/pUC) and uses a FAM-labeled target probe for quantitative detection and a VIC-labeled Internal Positive Control (IPC) included in the Primer\u0026amp;Probe MIX to monitor reaction performance. The kit provides reagents and a lyophilized plasmid linear DNA control for generation of a standard curve, enabling reliable quantitation down to the 10^2 copies\/µL level. The kit is supplied for 100 reactions and is intended for research use only. Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 1×10^2 copies\/µL (sensitivity reported by manufacturer)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitative dynamic range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd\u003eSerial dilution standard curve covers 4.97×10^8 to 4.97×10^1 copies\/µL (prepared from stock A down through ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total (20 µL qPCR MIX + 10 µL sample\/standard\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Master MIX 15 µL + Plasmid Primer\u0026amp;Probe MIX (incl. IPC) 5 µL = 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extend; fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget probe: FAM (Plasmid-DNA); Internal Positive Control (IPC): VIC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold: Plasmid-DNA (FAM) = 0.05; IPC (VIC) = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NTC Ct should be ≥ (ST5 Ct + 2) or lab-specific; NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable for up to 24 months under recommended storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex qPCR with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (VIC) within the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition or reaction failure.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Reported detection sensitivity at the 10^2 copies\/µL level for trace plasmid DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConsensus plasmid sequence targeting:\u003c\/strong\u003e Assay targets consensual replicon sequences (ColE1\/pMB1\/pBR322\/pUC) to detect a broad range of common plasmid backbones.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents and controls:\u003c\/strong\u003e Includes lyophilized plasmid linear DNA control and pre-mixed Primer\u0026amp;Probe and Master MIX reagents to streamline setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480 and similar platforms).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow for regulated testing environments:\u003c\/strong\u003e Provides recommended standard curve design, plate layouts, and acceptance criteria to support method validation and sample testing.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual plasmid DNA in gene therapy products\u003c\/li\u003e\r\n \u003cli\u003eMonitoring plasmid DNA residue in lentiviral vector preparations (CAR‑T production workflows)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for biologics where plasmid backbone contamination must be measured\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for plasmid removal\/clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid linear DNA Control (NNA016), lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid Primer\u0026amp;Probe MIX (Incl. IPC) (NNC120), 500 µL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023), 850 µL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and observe all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves) when handling reagents and samples.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention tubes and tips to avoid contamination and sample loss.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments to minimize nucleic acid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store all kit components at -20 °C. Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; clear cloudy DDB by warming to 37 °C if necessary.\u003c\/p\u003e\r\n \u003cp\u003e• Include recommended controls (NTC, NCS, ERC, and standard curve points) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the provided plate layouts, thermal profile and analysis settings; adjust thresholds\/criteria only after laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect residual plasmid DNA down to approximately 1×10^2 copies\/µL. Actual limit of detection should be confirmed by the user's in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the lyophilized Plasmid linear DNA Control in 55 µL ddH2O, dilute to the stock concentration indicated on the tube, then perform serial 1:10 dilutions to generate standards A → ST5. Recommended standard points in the manual range from 4.97×10^8 to 4.97×10^1 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret ERC recovery and control criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC (extraction reference control) recovery rates should fall between 50% and 150%. NCS Ct should be larger than the mean Ct of the lowest standard and show VIC amplification; NTC Ct should be ≥ ST5 Ct + 2 (or per your lab validation).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore all kit components at -20 °C and protect light-sensitive reagents from light. Components are stable for up to 24 months when stored under recommended conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189159813301,"sku":"1101111-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Plasmid_DNA_Quantitation_Kit_3G.jpg?v=1742288363"},{"product_id":"mycobacteria-dna-extraction-kit-mycoshentek™","title":"Mycobacteria DNA Extraction Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eApproximately 50 minutes\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature or 2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycobacteria DNA Extraction Kit is designed for extraction of trace Mycobacteria DNA from a variety of biologic sample types. The kit supports direct extraction from sample volumes ≤400 μL or concentration of larger volumes by centrifugation prior to extraction. It is compatible with manual magnetic-particle based workflows and automated extraction using the rHCDpurify\/rDNApurify instrument. The kit workflow includes pretreatment, Proteinase K digestion, magnetic particle binding (with isopropanol), two wash steps (ethanol-based), and heat elution to produce PCR-ready DNA (minimum recommended eluate ≥40 μL). The kit is supplied for research use only and is intended for downstream molecular detection (e.g., real-time PCR) with MycoSHENTEK detection reagents or other PCR systems.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eProduct No.: 1503601; reagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eExtraction of Mycobacteria DNA from cell-free and cell culture samples for downstream molecular detection (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003eRecommended ≤400 μL per sample; if \u0026gt;400 μL concentrate to ≈400 μL by centrifugation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K\u003c\/td\u003e\r\n \u003ctd\u003eAdd 20 μL Proteinase K per sample during digestion\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLysis\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100 μL Lysis buffer; incubate at 55 °C for 60 min (digestion)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 μL Binding solution + 200 μL isopropanol + 50 μL Magnetic particles per sample; shake 5 min for binding\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWashes\u003c\/td\u003e\r\n \u003ctd\u003eWash 1: 700 μL Wash buffer A (ethanol added before use); Wash 2: 700 μL Wash buffer B (70% ethanol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 50 μL Elution buffer; incubate at 70 °C for 7 min; recover at least 40 μL eluate\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomated run time\u003c\/td\u003e\r\n \u003ctd\u003erHCDpurify \/ rDNApurify program: ~50 minutes (plate-based automated run)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCentrifugation speeds \/ times\u003c\/td\u003e\r\n \u003ctd\u003eCell-free sample concentration: 16000×g for 15 min; Cell pelleting: 72×g for 5 min; Debris precipitation: 450×g for 10–15 min; brief spins (3 s) used frequently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particle separation time\u003c\/td\u003e\r\n \u003ctd\u003eSeparation typically 3–5 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAir-dry time (post-wash)\u003c\/td\u003e\r\n \u003ctd\u003e30 s to 3 min depending on environment; avoid over-drying\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components storable under indicated conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTrace DNA extraction:\u003c\/strong\u003e Optimized to extract low-abundance (trace) Mycobacteria DNA from various biologic matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic bead-based purification:\u003c\/strong\u003e Magnetic particle chemistry provides efficient capture and wash-based purification compatible with manual and automated workflows.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Validated for manual tube-based extraction and automated plate-based extraction using rHCDpurify \/ rDNApurify instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePCR-ready eluate:\u003c\/strong\u003e Produces a concentrated eluate (recommended ≥40 μL) suitable for downstream real-time PCR detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRoom-stable major reagents:\u003c\/strong\u003e Most reagents are stored at room temperature; selected reagents (e.g., cell lysis\/pretreatment\/magnetic particles) refrigerated as indicated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow reagents included:\u003c\/strong\u003e Includes lysis, binding, wash, elution buffers, proteinase K, magnetic particles and pretreatment reagents for sample types.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell-free biologics (e.g., serum, plasma, cell-free supernatants)\u003c\/li\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell culture samples and cell pellets\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for downstream molecular assays including real-time PCR\/qPCR\u003c\/li\u003e\r\n \u003cli\u003eResearch applications and in-process quality control testing of biologics (Research Use Only)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) — add 20 mL anhydrous ethanol before use\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 15 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Cell lysis buffer (NND039)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eFollow label; 500 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePretreatment buffer (NND041)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND034)\u003c\/span\u003e\u003cstrong\u003eFollow label (equilibrate to room temp before use); 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND024)\u003c\/span\u003e\u003cstrong\u003eFollow label; 1 mL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle-based DNA extraction (manual or automated)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and use appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Use separate, dedicated laboratory areas and equipment for reagent preparation, sample preparation, amplification, and post-amplification to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure ambient temperature ≥22 °C before starting experiments when recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Balance and vortex magnetic particles before use; centrifuge tubes briefly (3 s) when instructed to collect liquid from caps\/walls.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Wash buffer A by adding 20 mL anhydrous ethanol before first use; prepare Wash buffer B as 70% ethanol separately.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry the magnetic particle pellet after ethanol wash; over-drying may make resuspension difficult.\u003c\/p\u003e\r\n \u003cp\u003e• Perform mycobacterial DNA detection assays the same day as extraction to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• For automated runs: perform UV sterilization of the instrument for at least 15 min and clean internal walls with 75% alcohol wipes before and after runs; minimum interval between extractions is 30 min.\u003c\/p\u003e\r\n \u003cp\u003e• After automated extraction, immediately transfer eluate to a new tube and ensure ≥40 μL is recovered for downstream assays.\u003c\/p\u003e\r\n \u003cp\u003e• Dispose of used tips and liquid waste according to laboratory biohazard and local regulations.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for cell-free samples (e.g., serum, plasma, supernatants) and cell culture samples (cell pellets\/resuspensions). Samples ≤400 μL can be processed directly; larger volumes should be concentrated to ≈400 μL before extraction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended elution volume and expected yield for downstream PCR?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandard elution uses 50 μL Elution buffer with incubation at 70 °C for 7 minutes. Recover at least 40 μL eluate for downstream PCR\/qPCR applications.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I run the kit on an automated instrument?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with rHCDpurify \/ rDNApurify automated plate-based instruments. The automated program run time is approximately 50 minutes for a typical plate protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should magnetic particles be handled before use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eEquilibrate magnetic particles to room temperature for 10 minutes and vortex thoroughly to fully resuspend particles before aliquoting to samples to ensure consistent binding performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — For research purposes; not for diagnostic use\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45189176230069,"sku":"1503601","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Extraction_Kit.jpg?v=1742292295"},{"product_id":"mycobacteria-dna-detection-kit-mycoshentek™","title":"Mycobacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK® Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, and similar materials using real-time qPCR technology. The integrated system has been validated with a limit of detection of 10–100 CFU\/mL and can detect more than 100 different Mycobacteria species. The assay chemistry includes dUTP and is compatible with UNG (uracil-N-glycosylase) treatment to prevent carry-over contamination. An Internal Control (IC) is provided to monitor PCR inhibition and\/or extraction efficiency. The kit is supplied with positive and negative controls and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd\u003e1503602\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit Format \/ Reactions\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 Reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQualitative detection of Mycobacteria contamination in cells and cell culture-derived biologicals\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated at 10–100 CFU\/mL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n \u003ctd\u003eValidated to detect \u0026gt;100 Mycobacteria species\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay type\u003c\/td\u003e\r\n \u003ctd\u003eReal-time qPCR (probe-based)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL (10 µL qPCR MIX + 20 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eMB qPCR Reaction Buffer 8 µL; MB Primer \u0026amp; Probe MIX 1.5 µL; Internal Control (IC) 0.5 µL; total 10 µL. UNG optional (0.1 U per reaction).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG 25 °C 10:00 (1 cycle); Activation 95 °C 10:00 (1 cycle); 45 cycles of Denaturation 95 °C 00:15, Annealing 60 °C 00:30, Extension 72 °C 01:30 (read fluorescence during extension).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls\u003c\/td\u003e\r\n \u003ctd\u003ePositive Control (PC), No Template Control (NTC), Negative Control Sample (NCS), Internal Control (IC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\r\n \u003ctd\u003eTypical interpretation: FAM positive cutoff Ct \u0026lt;40.00 (positive if at least one well Ct\u0026lt;40 and normal amplification); VIC (IC) expected Ct \u0026lt;35.00 for normal amplification; PC Ct \u0026lt;35.00. NTC FAM Ct ≥40.00 or no amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, and other real-time PCR systems supporting probe detection\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Validated limit of detection down to 10–100 CFU\/mL for broad Mycobacteria detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed and validated to detect over 100 different Mycobacteria species.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarry-over contamination control:\u003c\/strong\u003e Chemistry includes dUTP and is compatible with UNG treatment to prevent and eliminate carry-over contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IC):\u003c\/strong\u003e IC monitors PCR inhibition and\/or extraction efficiency; can be added pre-extraction or to the PCR mix.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-validated workflow:\u003c\/strong\u003e Includes positive and negative controls and recommended plate layouts and acceptance criteria for straightforward result interpretation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQualitative detection of Mycobacteria contamination in cell lines\u003c\/li\u003e\r\n \u003cli\u003eScreening of cell culture-derived biologicals for Mycobacteria contamination\u003c\/li\u003e\r\n \u003cli\u003eRoutine mycoplasma\/contaminant monitoring workflows in research laboratories (Mycobacteria-specific)\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of Mycobacteria DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB qPCR Reaction Buffer (NNB011)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light; 400 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Primer \u0026amp; Probe MIX (NNC035)\u003c\/span\u003e\u003cstrong\u003e2–8 °C until use or -20 °C; 75 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Internal Control (IC) (NNA037)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or -20 °C; 600 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Positive Control (PC) (NNA041)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or room temperature per label; 1.5 mL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use UV decontamination (30 minutes) and 75% ethanol to clean surfaces and equipment before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use UNG enzyme optionally to reduce carry-over contamination (check effective amount before use).\u003c\/p\u003e\r\n \u003cp\u003e• If VIC (IC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout and include PC, NTC and NCS as described; use duplicates for samples and controls.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe integrated system has been validated with a limit of detection of approximately 10–100 CFU\/mL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the kit detect multiple Mycobacteria species?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The assay has been validated to detect more than 100 different Mycobacteria species.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is an Internal Control provided and how should it be used?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAn Internal Control (IC) is provided. It can be added during sample extraction to monitor extraction efficiency or added to the PCR mix to monitor PCR inhibition. If IC was added during extraction, replace IC volume with DNA dilution buffer when preparing the qPCR mix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended instruments and reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500). Prepare 30 µL reaction volumes (10 µL qPCR MIX + 20 µL sample\/control) and run the recommended cycling program with UNG treatment if used.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"Default Title","offer_id":43795617874101,"sku":"1503602","price":1525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Detection_Kit.jpg?v=1742292467"},{"product_id":"mycoplasma-dna-extraction-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature \/ 2–8 °C \/ -20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) is a magnetic particle–based DNA extraction kit designed for isolation of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks and complex sample matrices (e.g., up to 10^7 cells culture, 5% human albumin solutions, high-concentration plasmids). The kit is supplied for 50 extractions and is intended to be used in an integrated workflow together with the MycoSHENTEK® Mycoplasma Detection Kit (2G). The method is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection. The kit supports manual extraction and automated extraction using the rHCDpurify system. The validated detection limit is 10 CFU\/mL. The protocol includes sample pretreatment, enzymatic digestion (Proteinase K), magnetic-particle binding, wash steps, and elution to produce purified DNA suitable for downstream real-time PCR assays.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended use \/ scope\u003c\/td\u003e\n \u003ctd\u003eExtraction of trace mycoplasma DNA from cell banks, virus seed banks and complex matrices (cell cultures, 5% human albumin, high-concentration plasmids).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidated standards\u003c\/td\u003e\n \u003ctd\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated when used with MycoSHENTEK® Mycoplasma Detection Kit (2G)).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample input volume\u003c\/td\u003e\n \u003ctd\u003eUp to ~400 µL directly; larger volumes should be concentrated by centrifugation and reduced to ~400 µL prior to extraction.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 50 extractions.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTypical digestion\/incubation conditions\u003c\/td\u003e\n \u003ctd\u003eAdd 20 µL Proteinase K and 100 µL Lysis buffer; incubate 55°C for 60 min for most matrices; for 5% human albumin incubate at 25°C for 60 min.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eBinding step\u003c\/td\u003e\n \u003ctd\u003ePer sample add 200 µL Binding Buffer, 200 µL isopropanol and 30 µL Magnetic particles; vertical mixing ~5 min to bind nucleic acids.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eWash steps\u003c\/td\u003e\n \u003ctd\u003eTwo washes: 700 µL Wash buffer A then 700 µL Wash buffer B; remove residual ethanol and air-dry magnetic pellet briefly (30 s–3 min).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eElution\u003c\/td\u003e\n \u003ctd\u003eElute with 50 µL Elution buffer; incubate at 70°C for 7 min with intermittent vortexing; recover eluate (~40 µL+ recommended for downstream assay).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAutomated run time (rHCDpurify)\u003c\/td\u003e\n \u003ctd\u003eApprox. 43 minutes per plate run (program: Myco-2G).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage stability\u003c\/td\u003e\n \u003ctd\u003eKit components stable up to 24 months when stored under recommended conditions (see component list).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCritical centrifugation speeds\u003c\/td\u003e\n \u003ctd\u003eDebris removal: 7,250×g (10 s); concentration and plasmid\/cell concentration steps: 18,000×g (30 min); microcentrifuge brief spins: 3–10 s for liquid collection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls\u003c\/td\u003e\n \u003ctd\u003eNegative control sample (NCS) procedure provided; Internal Control (IC) (2G) is used during extraction (IC is supplied in the companion detection kit).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated detection limit of 10 CFU\/mL when used with MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad matrix compatibility:\u003c\/strong\u003e Works with cell cultures (up to 10^7 cells), cell culture supernatants, 5% human albumin solutions, high-concentration plasmids and other complex matrices.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports both manual magnetic-particle extraction and automated extraction on the rHCDpurify system.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeial validation:\u003c\/strong\u003e Validated in accordance with USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection workflows.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eComplete extraction chemistry:\u003c\/strong\u003e Includes lysis, binding, wash and elution reagents plus magnetic particles and proteinase K for robust nucleic acid recovery from low-copy targets.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eMycoplasma testing of master cell banks (MCBs) and working cell banks (WCBs)\u003c\/li\u003e\n \u003cli\u003eMycoplasma testing of virus seed banks\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture samples (up to 10^7 cells)\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture supernatants\u003c\/li\u003e\n \u003cli\u003eTesting of samples containing 5% human albumin\u003c\/li\u003e\n \u003cli\u003eExtraction from high-concentration plasmid preparations prior to mycoplasma detection\u003c\/li\u003e\n \u003cli\u003eSample preparation prior to real-time PCR-based mycoplasma detection\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (after adding 20 mL anhydrous ethanol prior to first use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (heat at 37°C if cloudy)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePretreatment buffer (NND002) — 1.25 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMagnetic particles (NND033) — 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003e5 M NaCl (NND040) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution I (NND003) — 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature (dilute 1:99 in DNA Dilution Buffer prior to use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution II (NND004) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eProteinase K (NND023) — 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C \/ according to label (keep frozen until use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based DNA extraction (magnetic bead chemistry) for downstream qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• Read MSDS and use appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\n \u003cp\u003e• Divide lab into separate areas (negative\/preparation, positive\/sample prep, amplification) and use dedicated equipment to prevent cross-contamination.\u003c\/p\u003e\n \u003cp\u003e• Ensure ambient temperature is not lower than 22°C before starting experiments (per manual recommendation).\u003c\/p\u003e\n \u003cp\u003e• Centrifuge tubes before opening to avoid splashing; handle tubes carefully to prevent contamination.\u003c\/p\u003e\n \u003cp\u003e• Change gloves frequently and use different gloves\/lab coats in separate areas.\u003c\/p\u003e\n \u003cp\u003e• Dispose of used tips and liquid waste according to local regulations; do not open post-PCR products in amplification area.\u003c\/p\u003e\n \u003cp\u003e• Avoid over-drying magnetic particle pellet during ethanol removal to prevent incomplete elution.\u003c\/p\u003e\n \u003cp\u003e• Perform DNA detection the same day as extraction to ensure accuracy.\u003c\/p\u003e\n \u003cp\u003e• For automated runs on rHCDpurify: UV sterilize the instrument for ≥15 minutes and clean inner walls with 75% ethanol wipes before and after runs; minimum interval between extractions should be 30 minutes.\u003c\/p\u003e\n \u003cp\u003e• When removing supernatants, avoid aspirating magnetic particles; use magnetic stand and allow full particle separation (3–5 min) before removing supernatant.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection when this extraction kit is used with the MycoSHENTEK® Mycoplasma Detection Kit (2G)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe workflow is validated with a detection limit of 10 CFU\/mL when used with the MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I process sample volumes larger than 400 µL?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. For sample volumes \u0026gt;400 µL the protocol requires concentration by centrifugation to a final volume of approximately 400 µL before extraction.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which samples require a different digestion temperature?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMost sample types are incubated with Proteinase K at 55°C for 60 minutes. Samples containing 5% human albumin and their controls should be incubated at 25°C for 60 minutes.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the kit compatible with automation?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with automated extraction using the rHCDpurify system (example program Myco-2G, ~43 minutes runtime).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45191334133941,"sku":"1509840","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Extraction_Kit_2G.jpg?v=1742362510"},{"product_id":"mycoplasma-dna-detection-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n 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center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20°C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR-based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culture–derived products. The kit is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII, with a stated limit of detection of 10 CFU\/mL. The assay detects \u0026gt;200 species with high specificity and includes Internal Control (IC, 2G) to monitor PCR inhibition and optionally to evaluate extraction efficiency when added prior to extraction. The kit is supplied for 50 reactions and is intended for research use only (RUO).\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eAssay type\u003c\/td\u003e\n \u003ctd\u003eQualitative real-time PCR (qPCR) assay\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\n \u003ctd\u003eDetects \u0026gt;200 species of Mycoplasma, Spiroplasma and Acholeplasma\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls included\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eInternal Control (IC) (2G), Positive Control (PC) (2G)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction format \u0026amp; volume\u003c\/td\u003e\n \u003ctd\u003e30 μL total reaction volume: 10 μL qPCR MIX + 20 μL sample (per well)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR Mix composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eMy qPCR Reaction Buffer (2G) 8 μL; My Primer \u0026amp; Probe MIX (2G) 1.5 μL; Internal Control (IC) (2G) 0.5 μL (total 10 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n \u003ctd\u003eUNG 25°C 10:00 (1); Activation 95°C 10:00 (1); 45 cycles of: Denaturation 95°C 00:15, Annealing 62°C 00:30, Extension 72°C 01:30 (fluorescence read during extension)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection channels \/ dyes\u003c\/td\u003e\n \u003ctd\u003eTarget: FAM; Internal Control: VIC; ROX optional as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, Roche LightCycler 480 and other real-time PCR systems\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended sample types \/ matrices\u003c\/td\u003e\n \u003ctd\u003eMaster cell bank, working cell bank, virus seed stock, cell culture-derived products and similar matrices (validated across a variety of matrices)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n \u003ctd\u003e-20°C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReconstitution instructions (controls)\u003c\/td\u003e\n \u003ctd\u003eReconstitute lyophilized IC (2G) in 600 μL DNA Dilution Buffer; reconstitute lyophilized PC (2G) in 500 μL DNA Dilution Buffer\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\n \u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35; PC: FAM Ct \u0026lt; 35; Unknown positive: FAM Ct \u0026lt; 40 (≥1 well) with normal amplification; If VIC inhibited, repeat extraction or remove inhibitors\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated LOD of 10 CFU\/mL allowing detection of low-level mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed to detect more than 200 Mycoplasma, Spiroplasma and Acholeplasma species with high specificity\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition and extraction monitoring:\u003c\/strong\u003e Internal Control (IC, 2G) can be added to PCR or to the sample prior to extraction to monitor PCR inhibition and evaluate extraction efficiency\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeia standards:\u003c\/strong\u003e Validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Provided My Primer \u0026amp; Probe MIX (2G) simplifies assay setup; lyophilized positive and internal controls included\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (SHENTEK-96S, CFX96, 7500, LightCycler 480, etc.)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQualitative mycoplasma testing of master cell banks and working cell banks\u003c\/li\u003e\n \u003cli\u003eScreening of virus seed stocks for mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture–derived products and biologics for mycoplasma\u003c\/li\u003e\n \u003cli\u003eRoutine cell culture contamination monitoring and QC in R\u0026amp;D and production (research use only)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eInternal Control (IC) (2G) (Part No. NNA035) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 600 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePositive Control (PC) (2G) (Part No. NNA039) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 500 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy qPCR Reaction Buffer (2G) (Part No. NNB004) 400 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy Primer \u0026amp; Probe MIX (2G) (Part No. NNC065) 75 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eUser Guide \/ Instructions for use\u003c\/span\u003e\u003cstrong\u003eRoom temperature (follow handling instructions)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For research use only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean area: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\n \u003cp\u003e• Thaw kit components at 2–8°C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n \u003cp\u003e• Protect My qPCR Reaction Buffer from light during storage and use.\u003c\/p\u003e\n \u003cp\u003e• If Internal Control (IC) was added during extraction, substitute equal volume of DNA Dilution Buffer in qPCR mix instead of IC.\u003c\/p\u003e\n \u003cp\u003e• Follow recommended plate layout and include controls (PC, NTC, NCS) in duplicate.\u003c\/p\u003e\n \u003cp\u003e• If VIC (IC) signal is inhibited (Ct ≥ threshold), repeat extraction or remove inhibitors and repeat assay.\u003c\/p\u003e\n \u003cp\u003e• Dispose of biological and chemical waste according to local regulations.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated with a limit of detection of 10 CFU\/mL.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the Internal Control (IC) be added before extraction?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. IC (2G) may be added to the sample prior to extraction to evaluate extraction efficiency; if IC is added during extraction, omit IC when preparing the qPCR mix and add equal volume of DNA Dilution Buffer instead.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which qPCR instruments are compatible with this kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500 and Roche LightCycler 480. Use instrument software to set FAM for target and VIC for IC.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted (Ct thresholds)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eAcceptance criteria: Positive control (PC) FAM Ct \u0026lt; 35; NTC FAM Ct ≥ 40 or no amplification; Unknown sample: FAM Ct \u0026lt; 40 (at least one well) with normal amplification = Positive. If IC (VIC) is inhibited (VIC Ct ≥ threshold or absent), repeat extraction or remove inhibitors and retest.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45191337345205,"sku":"1509841","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Detection_Kit_2G.jpg?v=1742362689"},{"product_id":"residual-vero-dna-quantitation-kit-shentek™","title":"Residual Vero DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Vero DNA Quantitation Kit is designed for quantitative measurement of residual Vero host cell DNA in biopharmaceutical samples across processing stages, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) with a Vero-specific primer \u0026amp; probe mix to deliver rapid, specific and reliable quantitation down to the femtogram (fg) level. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit provides reagents sufficient for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (Standard curve \/ Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at the femtogram (fg) level (document states femtogram-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST0 (3000 pg\/µL prepared stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL; ST6 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL; Sample\/Standard\/NTC\/NCS addition: 10 µL; Total reaction volume: 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + Vero Primer\u0026amp;Probe MIX 3 µL = 20 µL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector \/ Dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: (none specified)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected; Threshold for Vero-DNA set to 0.02; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ controls\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard; ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycles\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments (examples)\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 (other real-time PCR systems likely compatible after instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed for quantitation down to the femtogram (fg) level for detection of trace residual Vero DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Includes qPCR Reaction Buffer and Vero-specific Primer \u0026amp; Probe MIX to simplify setup and standardization.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePredefined standard curve:\u003c\/strong\u003e Provided Vero DNA Control and recommended serial dilutions (ST1–ST6) for absolute quantitation and standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example instrument settings provided for common real-time PCR systems; parameters can be adjusted per instrument\/software.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControl strategy and QC guidance:\u003c\/strong\u003e Includes instructions for NTC, NCS, and Extraction Reference Control (ERC) with recovery acceptance criteria (50%–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing during biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in Vero cell lines\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 µL × 2 tubes); protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX (NNC016)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and maintain a DNA-free workspace (UV decontamination and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light during storage and handling.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run at least five concentrations on the standard curve; include NTC, NCS and ERC controls as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; ERC recovery should be between 50% and 150%; NCS Ct must be greater than mean Ct of lowest standard or less than validated LOQ if LOQ \u0026lt; lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Configure instrument-specific analysis parameters according to your instrument\/software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the kit sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended to quantify residual Vero DNA down to the femtogram (fg) level. The validated limit of quantitation (LOQ) should be determined by laboratory validation; if LOQ is below the lowest standard, ensure NCS Ct is less than the LOQ concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents sufficient for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended storage conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C. The components are stable for up to 24 months; check individual tube labels for expiration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which qPCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems. The user guide provides example settings for SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and LineGene 9600. For other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187363700917,"sku":"SK030204V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Quantitation_Kit.png?v=1742278593"},{"product_id":"residual-sv40-lta-e1a-dna-quantitation-kit-2g","title":"Residual SV40 LTA\/E1A DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual SV40LTA \u0026amp; E1A DNA Quantitation Kit (2G) is a duplex real-time PCR (qPCR) kit designed to quantify residual SV40 large T antigen (LTA) and E1A host cell DNA (e.g., from HEK293T). The assay is duplex (simultaneous detection of SV40LTA and E1A) with an internal positive control (IPC) and achieves sensitivity to the level of 10^1 copies\/μL. The kit includes linear and non-linear DNA controls for standard curve generation and is compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96). Reagents are provided for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual SV40LTA and E1A host cell DNA in biological samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real-time PCR (qPCR) with target-specific primer\/probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10^1 copies\/μL (stated detection level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 4.67 × 10^6 to 4.67 × 10^1 copies\/μL (ST1–ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (non-linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 2.80 × 10^6 to 2.80 × 10^2 copies\/μL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl concentrations (as supplied)\u003c\/td\u003e\r\n \u003ctd\u003eLinear DNA Control: SV40LTA 4.67 × 10^9 copies\/μL; E1A 4.97 × 10^9 copies\/μL. Non-linear DNA Control: SV40LTA 2.80 × 10^9 copies\/μL; E1A 2.98 × 10^9 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation using standard curve (user-prepared serial dilutions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup \/ volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL sample or standard). qPCR MIX per reaction: qPCR Reaction Buffer 15.9 μL, Primer \u0026amp; Probe MIX 2.8 μL, IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15; Annealing\/extension 60 °C 1:00; 40 cycles. Fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other real-time PCR instruments supported with appropriate settings)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set Standard Curve (Absolute Quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within ±1.0 of the NCS Ct(IPC); significant increase indicates potential inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance thresholds\u003c\/td\u003e\r\n \u003ctd\u003eSV40LTA detection value of NTC should be ≤ 14.32 copies\/μL; E1A detection value of NTC should be ≤ 17.79 copies\/μL (or use user-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR:\u003c\/strong\u003e Simultaneous quantitation of SV40LTA and E1A targets in the same reaction using target-specific probes and an IPC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects down to 10^1 copies\/μL enabling detection of low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use controls and mixes:\u003c\/strong\u003e Includes linear and non-linear DNA controls, qPCR reaction buffer, primer\/probe mix, and IPC mix to support standard curve generation and assay QC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Validated on common qPCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96) and adaptable to other real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-defined analysis settings:\u003c\/strong\u003e Recommended thermal cycling and analysis parameters (e.g., threshold = 0.02, manual Ct) provided to standardize quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual SV40LTA and E1A host cell DNA in cell substrates (e.g., HEK293T)\u003c\/li\u003e\r\n \u003cli\u003eResidual host cell DNA monitoring for upstream\/downstream bioprocessing and product release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and standard curve generation for absolute quantitation of SV40-derived sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A linear DNA Control (NNA019) — lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A non-linear DNA Control (NNA020) — 50 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A Primer \u0026amp; Probe MIX (NNC030) — 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) — 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after opening per protocol)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect surfaces with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Reconstitute lyophilized control carefully: add 55 μL ddH2O, mix and let stand 10 minutes; perform serial dilutions as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer \u0026amp; Probe MIX, IPC MIX) from light and store at –20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five concentration points for the standard curve; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• DDB: store remaining unused DDB at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant shifts indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: SV40LTA ≤ 14.32 copies\/μL and E1A ≤ 17.79 copies\/μL (or use user-defined validation criteria).\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters per instrument and software version; recommended settings include manual Ct and threshold = 0.02.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit reports sensitivity to the level of 10^1 copies\/μL. Actual LOD\/LOQ should be confirmed during your laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500 (SDS v1.4 example) and Bio-Rad CFX96. Other instruments may be used with appropriate program and analysis adjustments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the qPCR thermal profile and analysis?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended program: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 (read fluorescence during annealing\/extension). Analysis: Manual Ct with threshold = 0.02 and Automatic Baseline; use Absolute Quantitation (standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I include and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude NTC, NCS, and at least five standard curve points (ST1–ST5; ST6 optional for linear control). IPC mean Ct should be within ±1.0 of NCS. NTC detection values should be ≤ 14.32 copies\/μL for SV40LTA and ≤ 17.79 copies\/μL for E1A, or use your validated criteria.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45191440335029,"sku":"1403443","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_SV40_LTA_E1A_DNA_Quantitation_Kit_2G.webp?v=1742280022"},{"product_id":"animal-origin-residual-dna-sample-preparation-kit","title":"Animal-origin Residual DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit uses magnetic particle separation technology to extract residual DNA from a broad range of animal-derived biomaterials. The kit is designed for efficient and reproducible recovery of residual DNA from complex sample matrices including extracellular matrix, collagen and bio-absorbable membranes. Extracted DNA is compatible with downstream residual DNA quantitation by qPCR or PicoGreen assays. The kit supports manual sample preparation and automated extraction using the rHCDpurify system. Reagents are supplied for 50 extractions. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ Version\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1104193; User Guide Version: A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — residual DNA extraction from animal-derived biomaterials\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions \/ Extractions per kit\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eOverall kit shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check label for expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles storage\u003c\/td\u003e\r\n \u003ctd\u003e2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCold-stored reagents\u003c\/td\u003e\r\n \u003ctd\u003eGlycogen: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC-treated H2O\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion conditions\u003c\/td\u003e\r\n \u003ctd\u003eIncubate at 55 °C for 60 minutes (dry bath); adjust amount\/time for difficult samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (Do not add Yeast tRNA when using PicoGreen assay)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding step volumes\u003c\/td\u003e\r\n \u003ctd\u003eAfter adding binding buffer, add 200 µL isopropanol and 30 µL magnetic particles per sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL (per wash step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100–150 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 minutes, vortex intermittently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic separation time\u003c\/td\u003e\r\n \u003ctd\u003eParticle separation typically ~3–5 minutes on a magnetic stand\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomation compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with rHCDpurify automated extraction (rHCD-193 program described)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n \u003ctd\u003eNegative control sample (NCS, 1×PBS) and sample extraction recovery control (ERC) recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eEnvironmental conditions for instrument\u003c\/td\u003e\r\n \u003ctd\u003eAmbient temperature should not be lower than 22 °C for automated runs; UV sterilization recommended before\/after runs\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation:\u003c\/strong\u003e Uses magnetic particle technology for efficient nucleic acid binding and purification from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized for animal-derived biomaterials:\u003c\/strong\u003e Validated for complex matrices such as extracellular matrix, collagen and bio-absorbable membranes.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility with downstream assays:\u003c\/strong\u003e Extracted DNA is suitable for qPCR quantitation and PicoGreen analysis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports manual extraction and high-throughput automated extraction on the rHCDpurify system.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eIncluded controls and reagents:\u003c\/strong\u003e Includes reagents and recommended controls (NCS, ERC) to assess recovery and contamination.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eResidual DNA quantitation in animal-derived biomaterials\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of collagen, extracellular matrix products and bio-absorbable membranes\u003c\/li\u003e\r\n \u003cli\u003ePreclinical \/ manufacturing residual DNA assessment\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for qPCR or fluorescence-based DNA assays (PicoGreen)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) 15 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND020) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e1× PBS (NND042) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDEPC-treated H2O (NND045) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND025) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle separation-based DNA extraction (manual or automated with rHCDpurify)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read MSDS and wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 20 mL anhydrous ethanol to Wash buffer A before first use; prepare Wash buffer B as 70% ethanol with ddH2O.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution freshly; incubate samples at 55 °C for 60 min (adjust as needed).\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when using PicoGreen DNA quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly before use; avoid over-drying pellets—dry 30 s to 3 min depending on conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge after vortexing to collect liquid from tube cap and walls before magnetic separation.\u003c\/p\u003e\r\n \u003cp\u003e• Transfer eluate immediately after elution and perform downstream assays the same day to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; cold-stored reagents (e.g., glycogen) should be kept at -20 °C.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Which downstream assays are compatible with the extracted DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted DNA is compatible with qPCR-based residual DNA quantitation and fluorescence-based assays such as PicoGreen. Do not add Yeast tRNA if using PicoGreen.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended digestion protocol for samples?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution (20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC H2O per sample) and incubate at 55 °C for 60 minutes. Adjust enzyme amount and digestion time for difficult-to-digest or cross-linked samples.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should Wash buffer A be prepared before first use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 20 mL of anhydrous ethanol to Wash buffer A (NND015) before first use as instructed in the manual.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I run the kit on an automated platform?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify system (program rHCD-193). Follow the plate layout and column reagent volumes specified in the manual and perform UV sterilization as recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171044843701,"sku":"1104193","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Animal-origin_Residual_DNA_Sample_Preparation_Kit.jpg?v=1742277138"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-vaccines-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e2-8 °C \u0026amp; -20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003e\n Product Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle–based nucleic acid extraction kit specifically optimized for vaccine products. It is designed to stably and efficiently recover trace amounts of residual host-cell DNA from finished vaccines (e.g., Vero cell–derived rabies vaccines). The kit provides an integrated workflow when used with SHENTEK® host cell DNA qPCR detection kits and supports manual extraction or automated extraction using the rHCDpurify® system. Special sample processing methods can be provided for samples containing aluminum adjuvants or dextran.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct number\u003c\/td\u003e\n\u003ctd\u003eSK030206DM50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended reactions\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample input volume\u003c\/td\u003e\n\u003ctd\u003e100 µL test sample (per extraction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion reagents per sample\u003c\/td\u003e\n\u003ctd\u003e10 µL 5M NaCl, 30 µL Sample buffer (NND029), 15 µL Proteinase K (NND023)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion conditions\u003c\/td\u003e\n\u003ctd\u003eIncubate at 55 °C for 60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\n\u003ctd\u003e200 µL Binding solution (NND017) + 200 µL isopropanol + 10 µL Magnetic particles (NND030)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding procedure\u003c\/td\u003e\n\u003ctd\u003eVortex 5 minutes (medium speed); magnetic separation 3–5 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash volumes\u003c\/td\u003e\n\u003ctd\u003e700 µL Wash buffer A (with ethanol added) then 700 µL Wash buffer B (70% ethanol)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume and conditions\u003c\/td\u003e\n\u003ctd\u003e50–100 µL Elution buffer (NND019); incubate at 70 °C for 7 minutes (vortex 2–3 times during incubation)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\n\u003ctd\u003eResuspend magnetic particles before use; allow particles to separate 3–5 minutes on magnet; air-dry 1–3 minutes before elution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible automation\u003c\/td\u003e\n\u003ctd\u003erHCDpurify® automated extraction system (program rHCD-06DM50 \/ HCD-06DM50)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage stability\u003c\/td\u003e\n\u003ctd\u003eKit components stored under indicated conditions are stable up to 24 months (check label expiration)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCritical sample pH range\u003c\/td\u003e\n\u003ctd\u003epH 6.0–8.0 required; adjust with 1M HCl or 1M NaOH if outside range\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended controls\u003c\/td\u003e\n\u003ctd\u003eNegative Control Sample (NCS) and Extraction Recovery Control (ERC) (ERC spike recommended 2–10× amount found in unspiked sample)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use \/ Compliance\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — sample preparation for downstream host cell DNA qPCR assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation technology:\u003c\/strong\u003e Efficient capture and concentration of trace DNA using magnetic particles for high recovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVaccine-optimized workflow:\u003c\/strong\u003e Formulation and protocol optimized for finished vaccine matrices (e.g., Vero cell–derived rabies vaccine). Specific processing available for aluminum adjuvants or dextran-containing samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated with qPCR detection:\u003c\/strong\u003e Designed to work with SHENTEK® host cell DNA qPCR detection kits to provide a complete sample-to-result workflow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eManual and automated compatibility:\u003c\/strong\u003e Protocols for manual extraction and for automated processing on the rHCDpurify® system (96 deep-well format) to support throughput needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow-volume, trace DNA recovery:\u003c\/strong\u003e Optimized to obtain trace amounts of residual host-cell DNA stably and reproducibly from complex vaccine matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eSample pretreatment for residual host cell DNA testing in finished vaccines (e.g., Vero cell–derived rabies vaccine)\u003c\/li\u003e\n\u003cli\u003eQuality control testing during vaccine manufacture\u003c\/li\u003e\n\u003cli\u003eSample preparation prior to SHENTEK® host cell DNA qPCR assays\u003c\/li\u003e\n\u003cli\u003eAutomated high-throughput residual DNA extraction using rHCDpurify® system\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eSample buffer (NND029)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based nucleic acid extraction for downstream qPCR detection (manual or rHCDpurify automated)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Follow Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\n\u003cp\u003e• Use separate laboratory areas for negative reagent preparation, positive\/sample processing, and PCR amplification to prevent cross-contamination.\u003c\/p\u003e\n\u003cp\u003e• Ensure ambient temperature is not lower than 22 °C before starting experiments.\u003c\/p\u003e\n\u003cp\u003e• Centrifuge tubes before opening to avoid contamination; handle carefully to avoid splashing.\u003c\/p\u003e\n\u003cp\u003e• Change gloves regularly and use dedicated lab coats\/masks\/hair covers in different areas.\u003c\/p\u003e\n\u003cp\u003e• Dispose of used tips and liquid waste per regulations; do not open PCR amplification products in non-designated areas.\u003c\/p\u003e\n\u003cp\u003e• During magnetic separation, rotate tubes slowly to accelerate particle aggregation; avoid disturbing particles when removing supernatant.\u003c\/p\u003e\n\u003cp\u003e• Avoid over-drying magnetic particles after wash (over-drying can cause incomplete elution).\u003c\/p\u003e\n\u003cp\u003e• Ensure no magnetic particles or liquid remain on tube caps or walls; if present, briefly centrifuge.\u003c\/p\u003e\n\u003cp\u003e• Perform DNA detection on the same day as extraction to ensure accuracy.\u003c\/p\u003e\n\u003cp\u003e• Before automated runs, confirm 96-well plate and sleeve are secured; UV-sterilize the instrument interior and wipe with 75% ethanol before and after runs.\u003c\/p\u003e\n\u003cp\u003e• Minimum interval between two extractions on rHCDpurify® should be 30 minutes; UV sterilize instrument for at least 15 minutes between runs.\u003c\/p\u003e\n\u003cp\u003e• Collect more than 40 µL of eluate for downstream assay; transfer eluate immediately after automated run to new microcentrifuge tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is required per extraction?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eUse 100 µL test sample per extraction. The protocol specifies addition of 10 µL 5M NaCl and 30 µL Sample buffer prior to Proteinase K digestion.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the magnetic particles and Proteinase K be stored?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Store Proteinase K at -20 °C. Other buffers are stored at room temperature; kit components are stable under indicated conditions for up to 24 months (check label expiration).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit be used with automated extraction instruments?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify® automated system (program: rHCD-06DM50 \/ HCD-06DM50) using a 96 deep-well plate layout; the manual also provides program settings and plate layout guidance.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe low recovery of nucleic acids?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include missing ethanol in Wash buffer A, magnetic particles sticking to tube walls, low ionic strength, out-of-range sample pH, or incorrect storage of magnetic particles. Solutions: add ethanol to Wash buffer A per instructions, vortex\/heat to release particles from tube walls (70 °C for 2 min) and re-vortex, adjust ionic strength with 5M NaCl, adjust sample pH to neutral, and store magnetic particles at 2–8 °C (do not store at -20 °C).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171074105525,"sku":"SK030206DM50","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_Vaccines.jpg?v=1742277270"},{"product_id":"residual-hansenula-polymorpha-dna-quantitation-kit-shentek®","title":"Residual Hansenula polymorpha DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hansenula polymorpha DNA Quantitation Kit is designed to quantitatively detect and measure residual Hansenula polymorpha host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses fluorescent quantitative PCR (real-time qPCR) with a specific primer\/probe mix to provide rapid, specific and reliable quantitation at the femtogram (fg) level. The kit is supplied with DNA control material and reagents for sample dilution and qPCR reaction setup. For DNA extraction\/sample preparation procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Labeling\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ Yield\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (implied)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL to 300 pg\/µL (30 fg\/µL to 300 pg\/µL) — demonstrates femtogram-level detection sensitivity\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denature 95 °C 0:15 (40 cycles); Anneal\/Extend 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL (17 µL qPCR Reaction Buffer + 3 µL Primer\u0026amp;Probe MIX); Final reaction: 30 µL (add 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02; Automatic baseline; NTC Ct ≥ 35 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ recovery criteria\u003c\/td\u003e\r\n \u003ctd\u003eERC recovery acceptable range: 50%–150%; NCS Ct should be larger than mean Ct of lowest standard curve concentration (or less than LOQ if LOQ \u0026lt; lowest standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C; kit components stable for up to 24 months (check expiration on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Mx3000P, StepOne Plus, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual Hansenula polymorpha DNA at femtogram-level sensitivity (standard curve includes down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFluorescent real-time qPCR:\u003c\/strong\u003e Uses a validated primer \u0026amp; probe mix with FAM reporter for specific and rapid quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for quantitation:\u003c\/strong\u003e Includes DNA control, qPCR reaction buffer, primer \u0026amp; probe mix and DNA dilution buffer — sufficient reagents for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Protocols and settings provided for commonly used real-time PCR platforms; assay template provided for absolute quantitation (standard curve).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC and controls included:\u003c\/strong\u003e Guidance and materials for NTC, NCS and ERC controls; recommended acceptance criteria (NTC Ct ≥35; ERC recovery 50–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha DNA in final drug products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC testing for host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eResearch applications involving detection of Hansenula polymorpha DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha DNA Control (NNA008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha qPCR Reaction Buffer (NNB007)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 μL × 2 tubes) — protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha Primer \u0026amp; Probe MIX (NNC040)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate working surfaces, pipettes and tubes (UV irradiation 30 minutes recommended) and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C; check expiration dates.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer is cloudy, heat to 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (NTC, NCS, ERC) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Recommended qPCR analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35 cycles or undetermined; ERC recovery 50%–150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level detection; the provided standard curve covers 0.03 pg\/µL (30 fg\/µL) to 300 pg\/µL. Users should validate LOD\/LOQ in their own laboratory and instrument setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR program and settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 0:15 (denature) and 60 °C 1:00 (anneal\/extend, read fluorescence). Analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline. Adjust for your instrument if required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions of the provided DNA Control in DNA Dilution Buffer to generate at least five concentrations (example: 300, 30, 3, 0.3, 0.03 pg\/µL). Run each standard in triplicate for absolute quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are acceptable QC criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or Ct ≥ 35 cycles. NCS Ct should be larger than the mean Ct of the lowest standard or below the LOQ if LOQ is lower than the lowest standard. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171150553269,"sku":"SK030222HA100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hansenula_Polymorpha_DNA_Quantitation_Kit.jpg?v=1742277725"},{"product_id":"residual-saccharomyces-cerevisiae-dna-quantitation-kit-shentek®","title":"Residual Saccharomyces¬†cerevisiae DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Saccharomyces cerevisiae DNA Quantitation Kit is designed to quantify residual Saccharomyces cerevisiae DNA in biopharmaceutical materials across process stages from in-process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with analytical sensitivity at the femtogram (fg) level. For DNA extraction guidance, refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit supplies reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking standard range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standards prepared from DNA control: 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest standard (example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL (30 fg\/µL) included as lowest standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (document states fg-level sensitivity); practical detection guided by lowest standard and lab validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified; users should determine LOQ by laboratory validation. The lowest provided standard is 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR master mix per reaction 20 µL + 10 µL sample = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMaster mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eSaccharomyces cerevisiae qPCR Reaction Buffer 17 µL + Primer \u0026amp; Probe MIX 3 µL (total 20 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C 10:00 (1 cycle); Denature: 95 °C 00:15 (40 cycles); Anneal\/Extend: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dye \/ probe\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; no quencher specified in instructions (primer \u0026amp; probe mix provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold \/ analysis settings\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended Manual Ct threshold = 0.02; baseline = Automatic. NTC Ct should be ≥ 35.00 cycles or undetermined (or laboratory-specific criteria).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIncludes DNA control for standard curve, Negative Control Sample (NCS), No Template Control (NTC) and Extraction Reference Control (ERC). ERC recovery acceptance: 50%–150%. Ct(NCS) should be larger than mean Ct of lowest standard (or below LOQ if LOQ proven lower).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, samples and controls\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months when stored appropriately (see component-level storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended consumables\u003c\/td\u003e\r\n \u003ctd\u003eDNase-free low-retention tubes and tips; 96-well qPCR plates with sealing film or 8-strip PCR tubes with caps\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to detect Saccharomyces cerevisiae DNA down to the femtogram (fg) level; includes low-concentration standards (down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and reliable:\u003c\/strong\u003e Contains optimized primer \u0026amp; probe mix and qPCR reaction buffer for specific amplification and reproducible quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Supplies DNA control, qPCR reaction buffer and primer \u0026amp; probe mix to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated for use on multiple real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit components are stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eHost cell DNA residue testing during upstream\/downstream process development and release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike\/recovery experiments using ERC samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae DNA Control (Part No. NNA009) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae qPCR Reaction Buffer (Part No. NNB006) — 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae Primer \u0026amp; Probe MIX (Part No. NNC039) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and change tips frequently.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex to mix and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial dilution as instructed; include at least five concentrations for the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Triplicate measurements are recommended for standards, samples and controls.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35 or be undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery should be between 50% and 150%; verify extraction efficiency and assay performance.\u003c\/p\u003e\r\n \u003cp\u003e• Determine LOQ and assay validation parameters within your laboratory according to regulatory guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Agilent Mx3000PTM and StepOne Plus. If using other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the lowest standard concentration provided and does it represent the kit LOQ?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest provided standard is 0.03 pg\/µL (30 fg\/µL). The kit states fg-level sensitivity, but you should determine the limit of quantification (LOQ) for your matrix by laboratory validation; the provided lowest standard can be used as a reference point.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I handle and store unused DNA Dilution Buffer (DDB)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore remaining unused DDB at 2–8 °C. If the solution becomes cloudy or precipitated, warm to 37 °C until clear before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be ≥ 35 cycles or undetermined. Ct of NCS should be larger than the mean Ct of the lowest standard (or below LOQ if the LOQ is proven lower than the lowest standard). ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171176636597,"sku":"1101103","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Saccharomyces_Cerevisiae_DNA_Quantitation_Kit.jpg?v=1742277789"},{"product_id":"residual-cho-dna-quantitation-kit-shentek®","title":"Residual CHO DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit is designed for quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (absolute quantitation with a standard curve) to perform rapid and specific quantitation of residual DNA at the femtogram level. For nucleic acid extraction and sample preparation guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100). The kit is supplied as reagents for 100 reactions and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical products (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by quantitative PCR (standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard concentrations prepared from CHO DNA Control: ST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL; ST6 = 0.003 pg\/μL. (Implied dynamic range: 300 to 0.003 pg\/μL using provided standards.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eStandards include down to 0.003 pg\/μL (ST6). No explicit LOQ\/LOD numeric claim provided; users should validate limit of quantitation (QL) in their own laboratory.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or standard).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample (or 10 μL standard\/controls as described).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix provided\u003c\/td\u003e\r\n \u003ctd\u003eReady-to-use CHO qPCR MIX supplied (mix thoroughly before use); protect from light.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C for 10:00; 40 cycles of Denaturation 95 °C for 0:15 and Annealing\/Extension 60 °C for 1:00 (fluorescence read at 60 °C).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling count\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — ERC recovery\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecovery of Extraction Reference Control (ERC) samples should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — NTC \/ NCS\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct should be Undetermined or not less than 35.00 (or laboratory-specific criteria). NCS Ct should be larger than the mean Ct of the lowest standard; if proven QL is lower than minimum standard concentration, NCS detected value should be less than QL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 Plus, Mx3000P, qTOWER 3 G, StepOnePlus (consult instrument documentation for setup).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components may be stored under recommended conditions for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended analysis settings (example ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set task types for standard, unknown, NTC as described.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of detecting residual CHO DNA down to femtogram-level concentrations (standards provided to 0.003 pg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Provided CHO qPCR MIX formulated for absolute quantitation—minimizes preparation steps and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard curve control:\u003c\/strong\u003e Includes CHO DNA Control and DNA Dilution Buffer to prepare a 6-point standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR platforms; instrument-specific setup instructions included.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow guidance:\u003c\/strong\u003e User guide includes serial dilution protocol, plate layout examples, thermal cycling program, and acceptance criteria (ERC recovery, NTC\/NCS rules).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics production (host cell DNA monitoring)\u003c\/li\u003e\r\n \u003cli\u003eProcess development and purification monitoring for cell-derived products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing in research settings\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (Part No. NNA001)\u003c\/span\u003e\u003cstrong\u003e50 μL × 1 tube; store at –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO qPCR MIX (Part No. NNC001)\u003c\/span\u003e\u003cstrong\u003e1 mL × 2 tubes; store at –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL × 3 tubes; store at –20 °C (remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (absolute quantitation, standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eye protection, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and centrifuge briefly; mix CHO qPCR MIX thoroughly and protect from light.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific setup and analysis parameters; example settings are provided for ABI 7500 SDS v1.4 but may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at recommended temperatures; per USP \u0026lt;659\u0026gt; items with –20 °C recommendation should be stored within ±10 °C (–30 °C to –10 °C).\u003c\/p\u003e\r\n \u003cp\u003e• Validate limit of quantitation (QL) and other performance metrics in your laboratory prior to routine testing.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure NTC (no template control) is undetermined or Ct ≥ 35 and NCS meets acceptance criteria before reporting sample results.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity of the kit and how low can it quantify CHO DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve includes concentrations down to 0.003 pg\/μL (ST6). The kit enables femtogram-level detection, but users should perform laboratory validation to determine the assay’s limit of quantitation (QL) under their specific conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL CHO qPCR MIX + 10 μL sample or standard). Use 10 μL of purified test sample per reaction as described in the user guide.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should reagents be stored and what is kit stability?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore CHO DNA Control, CHO qPCR MIX and DNA Dilution Buffer at –20 °C (protect qPCR mix from light). Kit components are stable for up to 24 months; check expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC shows amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC (no template control) is amplified (Ct \u0026lt; 35 or determined), suspect contamination. Decontaminate workspace and consumables (UV, 75% ethanol), re-prepare master mixes and controls, and repeat the assay. Verify pipetting technique and use fresh aliquots of reagents.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171252527285,"sku":"SK030201C100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit.jpg?v=1742278019"},{"product_id":"residual-ns0-sp2-0-dna-quantitation-kit-shentek®","title":"Residual NS0 \u0026 SP2\/0 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual NS0\u0026amp;SP2\/0 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical matrices (in-process samples to final products). The kit provides NS0\u0026amp;SP2\/0 DNA Control as a reference standard and contains qPCR Reaction Buffer, NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX, and DNA Dilution Buffer. The assay is designed for sensitive, specific and rapid quantitation at the femtogram (fg) level. Reagents in this kit are sufficient for 100 reactions. For DNA extraction procedures refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR using a supplied standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; lowest kit standard = 0.003 pg\/µL (3 fg\/µL). Laboratory validation required to establish LOD\/LOQ.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL (ST0 stock: 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX 3 µL = 20 µL per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (data acquisition during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, Bio-Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥ 35 cycles or undetermined (or per lab validation); Ct of NCS should be \u0026gt; mean Ct of lowest standard or below validated LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable for up to 24 months when stored as instructed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReference \/ related product\u003c\/td\u003e\r\n \u003ctd\u003eFor extraction information see SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at femtogram (fg) level; lowest supplied standard 0.003 pg\/µL\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Supplies DNA control and primer\/probe mix for construction of standard curve and absolute quantitation\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX minimize assay setup variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use on multiple common real-time PCR platforms\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuality controls included:\u003c\/strong\u003e Includes NS0\u0026amp;SP2\/0 DNA Control for standard curve and guidance for NTC, NCS and ERC controls\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual NS0 host cell DNA in biopharmaceutical process intermediates and final drug substance\/formulation\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual SP2\/0 host cell DNA in biopharmaceutical samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA testing in QC and R\u0026amp;D (Research Use Only)\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 DNA Control (Part No. NNA021)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 µL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX (Part No. NNC029)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes (remaining unused DDB store 2–8 °C; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory clothing and gloves\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and dedicated workspace\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate workspace, pipettes and tubes with UV for 30 minutes and 75% ethanol before setup\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly prior to use\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates\/strips and spin briefly before running qPCR\u003c\/p\u003e\r\n \u003cp\u003e• Establish laboratory-specific LOQ\/LOD by validation; NTC Ct acceptance: ≥ 35 cycles or undetermined\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance: 50%–150%\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration standard provided in the kit and what sensitivity can I expect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest supplied standard (ST6) is 0.003 pg\/µL (3 fg\/µL). The kit is designed to enable quantitation at the femtogram level; however, actual limit of detection (LOD) and limit of quantitation (LOQ) should be established by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the NS0\u0026amp;SP2\/0 DNA Control and DNA Dilution Buffer at 2–8 °C. Dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to prepare ST1–ST6 (ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL). Include at least five concentrations on the standard curve for quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What qPCR cycling conditions and reaction setup should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction volume (20 µL qPCR mix + 10 µL sample). Cycling: Activation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 0:15 (40 cycles); Annealing\/Extension 60 °C for 1:00 with fluorescence read during this step. Prepare qPCR mix per reaction: 17 µL qPCR Reaction Buffer + 3 µL Primer \u0026amp; Probe MIX.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I interpret controls and QC criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35 (or per lab validation). NCS Ct should be larger than mean Ct of lowest standard in the curve or below validated LOQ. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171267436725,"sku":"SK030208N100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_NS0_SP2_0_DNA_Quantitation_Kit.jpg?v=1742278378"},{"product_id":"residual-vero-dna-154-quantitation-kit-shentek®","title":"Residual Vero DNA-154 Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA-154 Quantitation Kit is a research-use qPCR kit for quantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process to final products). The kit uses a fluorescent quantitative PCR (FAM reporter) assay with a pre-formulated primer \u0026amp; probe mix and Vero DNA Control standard to enable rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit supplies Vero DNA Control as a reference standard and DNA Dilution Buffer for standard curve preparation. For nucleic acid extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (TaqMan-style probe); absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (documented serial dilutions down to 0.0003 pg\/μL = 0.3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003ePrepared from Vero DNA Control: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction (20 μL qPCR MIX + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + Vero Primer \u0026amp; Probe MIX-154 3 μL = 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReporter \/ Reference dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR instrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus (and similar real-time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTriplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality criteria \/ acceptance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate should be 50%–150%; Ct of NTC should be ≥35 or undetermined; Ct of NCS should be larger than mean Ct of lowest standard (or below validated LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months when stored as instructed (-20 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating Vero host DNA down to the femtogram level using an optimized primer\/probe assay and standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes pre-formulated Vero Primer \u0026amp; Probe MIX-154 for consistent, specific detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSupplied reference standard:\u003c\/strong\u003e Vero DNA Control provided for preparation of an 8-point serial dilution standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with multiple qPCR platforms:\u003c\/strong\u003e Validated workflows and example settings included for several common real-time PCR instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eUser guidance for QC:\u003c\/strong\u003e Includes instructions for NTC, NCS, ERC controls and acceptance criteria (ERC recovery 50%–150%, NTC Ct ≥35).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of drug substance and drug product for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of downstream purification steps\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-154 (NNC018)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 μL × 1 tube; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance range: 50%–150%; ensure NTC Ct ≥35 or undetermined.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample). qPCR MIX per reaction is composed of 17 μL qPCR Reaction Buffer + 3 μL Vero Primer \u0026amp; Probe MIX-154.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve concentrations should I prepare?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the provided Vero DNA Control as follows: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL. Include at least five concentration points in the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be within 50%–150%. The Ct for NTC should be ≥35 or undetermined. The Ct for NCS should be greater than the mean Ct of the lowest standard (or below validated LOQ if LOQ is lower than the lowest standard).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus, and similar instruments. Adjust analysis settings to your instrument\/software as needed.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171280609461,"sku":"SK030227V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA-154_Quantitation_Kit.jpg?v=1742278786"},{"product_id":"residual-mdck-dna-quantitation-kit-shentek®","title":"Residual MDCK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Quantitation Kit is designed to quantitate residual MDCK host cell DNA in biopharmaceutical workflows, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) for rapid, specific and reliable absolute quantitation at the femtogram (fg) level. The kit includes a MDCK DNA control, primer \u0026amp; probe mix, reaction buffer and DNA dilution buffer. For nucleic acid extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical samples (in‑process and final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative PCR (qPCR), absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims detection at femtogram (fg) level; lowest provided standard concentration = 0.003 pg\/μL (3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Linear range (provided)\u003c\/td\u003e\r\n \u003ctd\u003eStandard concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03, 0.003 pg\/μL (ST0–ST6); at least five concentrations recommended for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Quantitation (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eLOQ not explicitly specified; protocol notes use of validated LOQ; if LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal qPCR reaction volume = 30 μL (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + MDCK Primer \u0026amp; Probe MIX 3 μL = 20 μL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real‑time PCR instruments (SHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; Negative Control Sample (NCS) Ct should be larger than mean Ct of lowest standard (or less than LOQ if validated LOQ is lower); ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as labeled Product No.: SK030209M100)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components storable under appropriate conditions up to 24 months; individual components stored at -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay readout \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eRecommended analysis: Manual Ct, threshold for MDCK‑DNA = 0.02, automatic baseline; set tasks (Standard, Unknown, NTC) and verify slope, intercept, R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual MDCK DNA down to the femtogram level (lowest provided standard 0.003 pg\/μL = 3 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Standard‑curve based absolute quantitation using supplied MDCK DNA control and serial dilutions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes validated MDCK Primer \u0026amp; Probe MIX to simplify assay setup and improve reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on multiple common real‑time PCR platforms (ABI 7500, CFX96, StepOne Plus, LineGene, Mx3000P, SHENTEK‑96S, etc.).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eKit format:\u003c\/strong\u003e Provides reagents for 100 reactions with pre‑measured components (control, buffer, primer\/probe mix and dilution buffer).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process development\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support for final biopharmaceutical products (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for MDCK host cell DNA detection\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) — 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX (NNC024) — 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or therapeutic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low‑retention consumables; decontaminate workspace and pipettes with UV (30 min) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly; store unused DNA Dilution Buffer at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light‑sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, ERC and standard curve in each run.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument\/software‑specific parameter settings; example settings provided for ABI 7500 with SDS v1.4.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest standard concentration provided and what is the claimed sensitivity?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided serial dilution scheme includes standards down to 0.003 pg\/μL (3 fg\/μL). The kit is intended to enable quantitation at the femtogram level.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 20 μL qPCR mix per reaction (17 μL qPCR Reaction Buffer + 3 μL Primer \u0026amp; Probe MIX) and add 10 μL sample\/standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample program: Activation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15 (40 cycles); Annealing\/extension 60 °C 1:00 with fluorescence read during this step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should be used for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than the mean Ct of the lowest standard (or below validated LOQ if applicable); ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171293880501,"sku":"SK030209M100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Quantitation_Kit.jpg?v=1742279376"},{"product_id":"residual-sf9-acnpv-dna-quantitation-kit-shentek®","title":"Residual Sf9 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9\u0026amp;AcNPV DNA Quantitation Kit is designed for quantitative measurement of residual Sf9 and AcNPV host cell DNA in biopharmaceutical materials at different processing stages (in-process to final products). The kit uses a multiplex quantitative PCR (qPCR) assay with internal positive control (IPC) to provide rapid, specific and reliable quantitation down to femtogram-level sensitivity. Supplied reagents include DNA control standards for Sf9 and AcNPV, primer\/probe mixes, IPC mix, qPCR reaction buffer and DNA dilution buffer. The kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A). Refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction guidance.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (Sf9)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e300 pg\/µL to 0.003 pg\/µL (standard curve points: 300, 30, 3, 0.3, 0.03, 0.003 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (AcNPV)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5.14×10^6 to 5.14×10^1 copies\/µL (equivalent mass: 20 to 0.0002 pg\/µL; standard curve points provided for both copies\/µL and pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity down to femtogram (fg) level implied by standard curve lowest points (Sf9: 0.003 pg\/µL = 3 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReaction total volume 30 µL per well (20 µL qPCR MIX + 10 µL sample).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL (total 20 µL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C for 10:00 (1 cycle); Denaturation: 95 °C for 0:15; Annealing\/Extension: 60 °C for 1:00 (read fluorescence). 40 cycles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument channels \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eSf9-DNA: FAM; AcNPV-DNA: CY5; IPC: VIC; Passive reference dye: ROX (ABI 7500 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ quantitation settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold 0.02 for Sf9-DNA, AcNPV-DNA and IPC; use Absolute Quantitation (Standard Curve) analysis.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC: Ct(sample) should be within ±1.0 Ct of Ct(NCS) — large deviation indicates inhibition. NTC: Ct ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as shipped).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see component-specific storage: most components -20 °C; protect qPCR Reaction Buffer from light).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ baseline guidance\u003c\/td\u003e\r\n \u003ctd\u003eThreshold set to 0.02; Automatic baseline recommended when using provided settings.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR:\u003c\/strong\u003e Simultaneous detection and quantitation of Sf9-DNA, AcNPV-DNA and IPC in one reaction using distinct fluorescent channels (FAM, CY5, VIC).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay standard curve includes femtogram-level concentrations (Sf9 down to 0.003 pg\/µL) enabling detection at fg scale.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC provided to monitor PCR inhibition and overall reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix, IPC mix and reaction buffer simplify setup and reduce pipetting errors.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated examples and guidance provided for multiple real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, CFX96, FQD-96A).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStandardized quantitation:\u003c\/strong\u003e Supplied Sf9 \u0026amp; AcNPV DNA controls with serial dilution scheme for generating absolute standard curves (copies\/µL and pg\/µL).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host cell DNA in biopharmaceutical process and final drug substance\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Autographa californica multiple nucleopolyhedrovirus (AcNPV) DNA in bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control and release testing for products produced in Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV DNA Control (NNA051), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX (NNC059), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC067), 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused remaining DDB may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free area; use DNase-free, low-retention consumables and tips.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate work surfaces and equipment for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial 10-fold dilutions in DDB as instructed; at least five standard concentrations recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates well, mix and spin down prior to starting qPCR to avoid evaporation and inconsistent Ct values.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC: sample Ct-IPC should be within ±1.0 Ct of NCS; significant increase indicates sample inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35.00 or be undetermined; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents for 100 reactions (stated on the product cover).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and thermal program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 30 µL reactions (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 with fluorescence read during the 60 °C step. Use manual Ct threshold 0.02 as guidance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than Ct-IPC of NCS (beyond ±1.0 Ct), consider sample dilution, re-extraction, or purification to remove inhibitors. Use recovery of spiked reference standards to assess sample processing.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the assay dynamic ranges and units?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSf9 standard curve: 300 to 0.003 pg\/µL. AcNPV standard curve: 5.14×10^6 to 5.14×10^1 copies\/µL (equivalent to 20 to 0.0002 pg\/µL). At least five standard concentrations should be used for curve generation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171362431157,"sku":"1101101","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_AcNPV_DNA_Quantitation_Kit.jpg?v=1742279477"},{"product_id":"residual-cv-1-dna-quantitation-kit-shentek-n","title":"Residual CV-1 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CV-1 DNA Quantitation Kit is designed to quantitate residual CV-1 host cell DNA across stages of biopharmaceutical production, from in-process samples to final products. The kit uses quantitative PCR (qPCR) to deliver rapid, specific and reliable quantitation at the femtogram (fg) level and includes a CV-1 DNA Control reference standard. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST0: 3000 pg\/µL; ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest included standard (indicative sensitivity)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.003 pg\/µL (3 fg\/µL) — kit supports femtogram-level quantitation; validated LOQ must be determined by laboratory validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (20 µL qPCR mix + 10 µL sample\/diluent)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer: 17 µL; CV-1 Primer \u0026amp; Probe MIX: 3 µL; Total qPCR MIX per reaction: 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C, 10:00 (1 cycle); Denaturation: 95 °C, 00:15 (40 cycles); Annealing\/Extension: 60 °C, 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection settings\u003c\/td\u003e\r\n \u003ctd\u003eRecommended reporter dye: FAM; passive reference: ROX (ABI7500 example). Manual Ct threshold example: 0.02 (Automatic baseline recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 cycles or undetermined (or per laboratory validation); NCS Ct should be larger than mean Ct of lowest standard (or below LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months at recommended storage conditions (component-specific temperatures listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims femtogram-level detection; lowest provided standard 0.003 pg\/µL (3 fg\/µL) allowing very low-level residual DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Absolute quantitation using a supplied CV-1 DNA Control standard curve (six dilution points recommended).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX to streamline assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComprehensive workflow support:\u003c\/strong\u003e Includes guidance for standard curve preparation, plate layout recommendations, qPCR program settings and result analysis instructions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in final drug substance\/drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in CV-1 cells\u003c\/li\u003e\r\n \u003cli\u003eProcess development and optimization where host-cell DNA monitoring is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 DNA Control (NNA026)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 Primer \u0026amp; Probe MIX (NNC097)\u003c\/span\u003e\u003cstrong\u003eStore as labeled (provided 300 μL × 1 tube); follow kit storage instructions\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C during use); 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation\u003c\/span\u003e\u003cstrong\u003eUser Guide and instructions included\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for human diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and pipettes with UV (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice (2–8 °C), vortex and spin briefly before use; protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh using serial dilutions; include at least five concentrations for curve fitting.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates with appropriate film, mix and spin briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC Ct ≥35 cycles or undetermined (or per lab validation); NCS Ct must be \u0026gt; mean Ct of lowest standard or below validated LOQ.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific program and detector settings must be configured per instrument\/software documentation (ABI7500 example provided).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity or lowest detectable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides a lowest standard of 0.003 pg\/µL (3 fg\/µL) and is designed to support femtogram-level quantitation. The validated limit of quantitation (LOQ) should be determined by laboratory validation and may be lower or higher depending on sample matrix and extraction recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should reagents be stored and handled?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures (major components at -20 °C). Thaw reagents on ice (2–8 °C), vortex and spin briefly before use. Protect light-sensitive components (e.g., qPCR Reaction Buffer) from light. Follow MSDS guidance and use DNase-free consumables.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should have Ct ≥35 cycles or be undetermined (or per laboratory validation). The NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve or below the validated LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are supported and what detector settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). In the ABI7500 example, create a FAM detector (reporter FAM), set ROX as passive reference, use Manual Ct threshold 0.02 and Automatic baseline; thermal cycling: 95 °C activation 10:00; 40 cycles of 95 °C 00:15 and 60 °C 1:00 (read fluorescence during anneal\/extension). Adjust settings per instrument and software version.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187376840885,"sku":"1101105","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CV-1_DNA_Quantitation_Kit.webp?v=1742279215"},{"product_id":"residual-hi5-acnpv-dna-quantitation-kit-shentek®","title":"Residual Hi5 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n 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8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hi5\u0026amp;AcNPV DNA Quantitation Kit is designed to quantify residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses a multiplex quantitative PCR (qPCR) assay with target-specific primer\/probe mixes for Hi5 DNA and AcNPV DNA plus an Internal Positive Control (IPC) to monitor reaction performance and potential inhibition. The assay supports absolute quantitation using a supplied lyophilized DNA control and DDB-based serial dilutions. The kit provides reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples across process stages\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMultiplex quantitative PCR (qPCR) with three detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 μL total per well (20 μL qPCR mix + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; Hi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eHi5 DNA standard curve (mass)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points: 30 pg\/μL (ST1), 3 pg\/μL (ST2), 0.3 pg\/μL (ST3), 0.03 pg\/μL (ST4), 0.003 pg\/μL (ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcNPV DNA standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points (copies\/μL and pg\/μL): ST1 5.14×10^5 copies\/μL (2 pg\/μL), ST2 5.14×10^4 (0.2 pg\/μL), ST3 5.14×10^3 (0.02 pg\/μL), ST4 5.14×10^2 (0.002 pg\/μL), ST5 5.14×10^1 (0.0002 pg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity claim\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level is supported by the provided standard curve down to 0.003 pg\/μL for Hi5 and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC Ct of sample vs NCS should be within ±1.0 Ct; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger (i.e., later) than mean Ct of lowest standard; NCS should show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample (per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable up to 24 months when stored as directed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided quantity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions (kit contains reagents sized as listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated LOQ is referenced in method validation; the user should determine LOQ during method validation. If LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR format:\u003c\/strong\u003e Simultaneous detection of Hi5 DNA, AcNPV DNA and IPC in a single reaction (FAM, CY5, VIC channels)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Supports quantitation down to femtogram-level concentrations using supplied standards (mass and copy-number options for AcNPV)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify inhibited samples\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer\u0026amp;Probe MIX and IPC MIX reduce assay setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hi5 host-cell DNA in biopharmaceutical process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual AcNPV DNA (baculovirus) in upstream and downstream process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support and method development for biologics produced in Hi5\/AcNPV systems\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV DNA Control (lyophilized powder) ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C (after opening\/for use, store remaining DDB at 2–8 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, mask, protective eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout recommendations and include at least five standard curve points, NTC and NCS in each run.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: Ct(sample IPC) must be within ±1.0 Ct of Ct(NCS) — higher IPC Ct indicates potential inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: Ct ≥ 35 or undetermined; NTC should not show target amplification.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Validated LOQ must be established during method validation; if LOQ is below the lowest standard, ensure NCS value is below LOQ.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration for Hi5 and AcNPV using this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve supports quantitation down to 0.003 pg\/μL for Hi5 DNA and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV DNA. Users should perform method validation to determine assay LOD\/LOQ for their matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample should be added per reaction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 10 μL of purified test sample per 30 μL reaction (20 μL qPCR mix + 10 μL sample).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the instrument settings for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal profile: Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 01:00 with fluorescence read at 60 °C. Set reaction volume to 30 μL. Configure detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC Ct is significantly higher than NCS IPC Ct (outside ±1.0 Ct), suspect inhibition. Consider diluting the sample, re-extracting with improved purification, or including spike-recovery controls to assess sample recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173391065269,"sku":"1101102","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hi5_AcNPV_DNA_Quantitation_Kit.png?v=1742279579"},{"product_id":"residual-human-dna-quantitation-kit-shentek®","title":"Residual Human DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Human DNA Quantitation Kit (2G) is a duplex quantitative PCR (qPCR) kit for quantitation of residual human host-cell DNA in biopharmaceutical samples from in-process to final products. The assay achieves femtogram-level sensitivity and includes an Internal Positive Control (IPC) in the Human Primer \u0026amp; Probe MIX to monitor PCR performance. The kit contains a Human DNA Control for standard curve generation and ready-to-use qPCR Master MIX and Primer\u0026amp;Probe MIX. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual human host-cell DNA in biopharmaceutical samples (in-process to final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex quantitative PCR (qPCR) with Human-DNA detector (FAM) and IPC (VIC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level; NTC detection value acceptance ≤ 6.00 fg\/μL (user may set criteria by method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical linear range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e300 pg\/μL to 0.03 pg\/μL (example standard series provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis thresholds (example)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-DNA = 0.05; IPC = 0.1; Automatic baseline\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate 50%–150%; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification; NTC detection ≤ 6.00 fg\/μL and IPC amplification normal\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePack size \/ yield\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions (product no. 1101108)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored at appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation at femtogram (fg) level\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes Internal Positive Control in the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and Primer\u0026amp;Probe MIX reduce setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR systems (SHENTEK-96S, ABI 7500, CFX96, LightCycler 480)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow convenience:\u003c\/strong\u003e Includes Human DNA Control and DNA Dilution Buffer with recommended serial dilution scheme for standard curve generation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human host-cell DNA in biopharmaceutical products (in-process and final product)\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring and release testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eComparative testing of extraction methods (use with Extraction Reference Control, ERC)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer \u0026amp; Probe MIX (includes IPC) (NNC130) 500 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReagents for 100 reactions (kit pack information)\u003c\/span\u003e\u003cstrong\u003e-20 °C (components stable up to 24 months)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination. UV decontaminate work surfaces and pipettes prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Master MIX from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, and ERC. Run standards (at least five concentrations) to build a standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC ≤ 6.00 fg\/μL (or criteria defined during method validation); NCS Ct larger than mean Ct of lowest standard and shows IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR analysis parameters (e.g., thresholds, baseline) should be configured for the specific instrument and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level quantitation. Example standard points include down to 0.03 pg\/μL (30 fg\/μL). The kit documentation states NTC detection acceptance ≤ 6.00 fg\/μL; true LOD\/LOQ should be determined by the user's method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Roche LightCycler 480. It is compatible with other real-time PCR instruments that support dual-channel detection (FAM and VIC), but instrument-specific setup is required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How many reactions are included and how should reagents be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Store components at -20 °C and protect the qPCR Master MIX from light. Components are stable up to 24 months—confirm expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I run and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRun a standard curve (minimum five points), Non-Template Control (NTC), Negative Control Sample (NCS), and Extraction Reference Control (ERC). ERC recovery should be between 50% and 150%. NTC detection should be ≤ 6.00 fg\/μL (or per user validation). NCS Ct should be larger than the mean Ct of the lowest standard and show IPC amplification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173398765749,"sku":"1101108","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Quantitation_Kit.png?v=1742279705"},{"product_id":"residual-bhk-dna-quantitation-kit-shentek®","title":"Residual BHK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Quantitation Kit is designed to quantitate residual BHK host cell DNA in different stages of biopharmaceutical products, from in‑process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit includes a BHK DNA Control and reagents for preparing standard curves and performing absolute quantitation. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical samples (in‑process to final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by fluorescent qPCR (standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects at the femtogram (fg) level (laboratory validation recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e3000 pg\/µL, 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (ST0–ST5 as prepared per protocol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + BHK Primer\u0026amp;Probe MIX 3 µL → 20 µL qPCR mix; add 10 µL sample\/standard to reach 30 µL total\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: none (per setup instructions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard (or larger than LOQ Ct if LOQ is below lowest standard); ERC recovery rate should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; stable up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reaction count\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents supplied for 100 reactions (kit format)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating residual BHK DNA at the femtogram (fg) level when properly validated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes BHK DNA Control and standard curve workflow for absolute quantitation of target DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Contains qPCR Reaction Buffer and Primer \u0026amp; Probe MIX optimized for the assay to reduce setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated for use on SHENTEK‑96S, ABI 7500, Roche LightCycler 480 II and other real‑time PCR systems (instrument settings may vary).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Intended for testing residual host cell DNA across in‑process samples to final product stages and for assay validation workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing for residual DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003cli\u003eQuality control and stability studies involving host cell DNA measurements\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (Part No. NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX (Part No. NNC076) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low retention consumables and clean work area (UV irradiation and 75% ethanol decontamination recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly spin before use; protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration date on the label.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points for the standard curve and perform triplicates for samples and controls where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery rates should be between 50% and 150%; investigate deviations.\u003c\/p\u003e\r\n \u003cp\u003e• Set instrument analysis parameters (thresholds, baseline, detector settings) according to instrument\/software and laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to enable quantitation at the femtogram (fg) level. The practical limit of detection (LOD) and limit of quantification (LOQ) should be established by laboratory validation. The provided standard curve includes concentrations down to 0.03 pg\/µL (30 fg\/µL as prepared).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real‑time PCR instruments. The user guide provides example settings for SHENTEK‑96S, ABI 7500 (SDS v1.4), and Roche LightCycler 480 II. Adjust instrument and software parameters as needed for other platforms and validate performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the BHK DNA Control and DNA Dilution Buffer at 2–8 °C, dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentration points and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC or NCS show amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC shows amplification (Ct \u0026lt; 35), verify reagent and plate handling to identify contamination sources, replace consumables, re‑prepare reagents, decontaminate work surfaces and equipment (UV\/ethanol), and repeat the assay. If NCS Ct is not larger than the lowest standard Ct or LOQ Ct, review extraction and dilution procedures and revalidate the LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173411610805,"sku":"1101116","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Quantitation_Kit.png?v=1742279915"},{"product_id":"residual-e1a-dna-quantitation-kit-shentek®","title":"Residual E1A DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1A DNA Quantitation Kit is designed for the quantitation of residual E1A DNA derived from host cells (e.g., HEK293 and 293T) across different stages of biopharmaceutical manufacture, from in-process samples to final products. The kit uses fluorescent quantitative PCR (real-time qPCR) for rapid, specific and reliable absolute quantitation. An Internal Positive Control (IPC) is included to monitor PCR performance and potential inhibition. The kit provides both E1A linear DNA Control and E1A non-linear DNA Control to accommodate different user needs. Recommended sample extraction procedures are provided in the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e4.97 × 10^8 copies\/μL (stock) with serial 10-fold dilutions down to 4.97 × 10^1 copies\/μL (ST1–ST6 covering 4.97 × 10^6 to 4.97 × 10^1 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (non-linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e2.98 × 10^7 copies\/μL (stock) with serial 10-fold dilutions down to 2.98 × 10^2 copies\/μL (ST0–ST5 covering 2.98 × 10^6 to 2.98 × 10^2 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; E1A Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample or purified NCS per reaction; recommended starting material for extraction: 100 μL sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection dyes\u003c\/td\u003e\r\n \u003ctd\u003eE1A-DNA detector: CY5; IPC detector: VIC; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for both E1A-DNA and IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt value of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; significant shift indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ \/ LOD\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ) not specified in kit documentation; users should determine proven LOQ during method validation. If proven LOQ is lower than lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended replicates and standard points\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended; at least five concentration points recommended for standard curve (six when using linear DNA control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eValidated instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other compatible real-time PCR systems may be used)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and sensitive absolute quantitation:\u003c\/strong\u003e Fluorescent qPCR assay for quantitative detection of residual E1A DNA with serial standards to create an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify potential sample inhibition; IPC results used to assess recovery when spiked samples are tested.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTwo control formats provided:\u003c\/strong\u003e Both E1A linear DNA Control (lyophilized) and E1A non-linear DNA Control are supplied so users can choose according to validation needs.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX supplied in ready-to-use formats to simplify setup; protect light-sensitive reagents from light.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, ABI 7500, CFX96); thermal and detector settings provided for instrument configuration.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1A DNA from host cells (e.g., HEK293, 293T) in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing and final product testing for residual host-cell E1A DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and QC workflows requiring absolute quantitation of E1A sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A linear DNA Control (lyophilized powder × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A non-linear DNA Control (50 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A Primer \u0026amp; Probe MIX (300 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow laboratory safety procedures; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration dates. Do not use if reagents show precipitate or cloudiness (heat DDB at 37 °C to clarify if necessary).\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, standards and IPC. Use triplicates for samples and standards for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific settings should be adapted to the user's instrument and software version; follow provided detector\/dye assignments and thermal profile as guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitation of residual E1A DNA from host cell-derived samples (for example, HEK293 and 293T) and is applicable to samples collected during biopharmaceutical manufacturing (in-process and final product). Samples must be purified prior to qPCR according to the recommended extraction workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is the standard curve prepared and what dynamic range is covered?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are prepared by serial 10-fold dilutions of the provided DNA controls. The linear DNA control stock is 4.97 × 10^8 copies\/μL (diluted down to 4.97 × 10^1 copies\/μL across ST1–ST6); the non-linear control stock is 2.98 × 10^7 copies\/μL (diluted down to 2.98 × 10^2 copies\/μL across ST0–ST5). At least five standard points (six for linear control) are recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What quality criteria should I use to accept a run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35. IPC Ct for sample should be within ±1.0 Ct of IPC Ct for NCS; significant shifts indicate inhibition. Verify standard curve slope, intercept and R^2 before reporting sample quantities.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the limit of quantification (LOQ) provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eA numeric LOQ is not specified in the kit documentation. Users should determine the proven LOQ during method validation for their specific matrix and instrument. If proven LOQ is below the lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173426094261,"sku":"1101109","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1A_DNA_Quantitation_Kit.png?v=1742280136"},{"product_id":"residual-e1b-dna-quantitation-kit-shentek®","title":"Residual E1B DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1B DNA Quantitation Kit is designed for absolute quantitation of residual E1B DNA derived from host cells (e.g., HEK293 and derivative cell lines such as 293T and 293F) in various stages of biopharmaceutical manufacturing (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (qPCR) assay with an internal positive control (IPC) to monitor PCR performance and includes an E1B linear DNA Control for preparation of standard curves and for detection of linearized samples. The kit is supplied for 100 reactions and is intended for research use only. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by fluorescent qPCR (FAM-labeled target, VIC-labeled IPC, ROX passive reference)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eST1 to ST6: 4.34 × 10^6 to 4.34 × 10^1 copies\/μL (prepared from E1B linear DNA Control; original ST = 4.34 × 10^8 copies\/μL before serial dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL, E1B Primer\u0026amp;Probe MIX 2.8 μL, IPC MIX 1.3 μL (total qPCR MIX = 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00; Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument settings \/ analysis\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 for both E1B-DNA and IPC; Automatic baseline; Standard curve (absolute quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control acceptance\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; a significantly higher Ct(IPC) indicates potential PCR inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input for preparation\u003c\/td\u003e\r\n \u003ctd\u003e100 μL test sample (or 100 μL DDB for negative control sample preparation prior to extraction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under stated conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification \/ limit of detection\u003c\/td\u003e\r\n \u003ctd\u003eNot numerically specified in the manual. Users should establish LOD\/LOQ by validation; standard curve lowest point in user protocol is 4.34 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh specificity and sensitivity:\u003c\/strong\u003e Fluorescent qPCR assay with sequence-specific primers and probe for E1B enabling specific detection of residual E1B DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Included IPC MIX monitors PCR performance and helps identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX minimize setup errors and speed assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eE1B linear DNA Control:\u003c\/strong\u003e Supplied lyophilized linear DNA control for preparation of serial dilutions and construction of an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on multiple real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, qTOWER3G, LightCycler480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFor residual host cell DNA testing:\u003c\/strong\u003e Designed specifically to quantitate residual E1B DNA from HEK293 and derivative cell lines in biopharmaceutical samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1B DNA in biopharmaceutical products (in-process and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in HEK293 and derivative cell lines (e.g., 293T, 293F)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host cell DNA analysis\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA clearance and downstream process effectiveness\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B linear DNA Control (lyophilized powder × 1 tube) — Part No. NNA014\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes) — Part No. NNB002\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B Primer \u0026amp; Probe MIX (300 μL × 1 tube) — Part No. NNC034\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube) — Part No. NNC066\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes) — Part No. NND001\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free consumables and low-retention tips to avoid contamination and loss.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer\u0026amp;Probe MIX, IPC MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup to reduce contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure lyophilized E1B linear DNA Control is fully dissolved (add 55 μL ddH2O and mix as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 or undetermined; check IPC Ct to assess inhibition (sample IPC Ct must be within ±1.0 Ct of NCS IPC Ct).\u003c\/p\u003e\r\n \u003cp\u003e• Establish assay-specific LOD\/LOQ during method validation; lowest manual standard = 4.34 × 10^1 copies\/μL.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses fluorescent quantitative PCR (qPCR) for absolute quantitation of E1B DNA (FAM-labeled target, VIC-labeled IPC, ROX passive reference).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit and what is the recommended reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. The recommended total reaction volume is 30 μL (20 μL qPCR MIX + 10 μL sample\/control).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the standard curve range included in the user protocol and is LOQ specified?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol prepares a standard curve with concentrations from 4.34 × 10^6 down to 4.34 × 10^1 copies\/μL (ST1–ST6). A numeric LOQ\/LOD is not specified in the manual; users should determine assay-specific LOD\/LOQ during validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIPC (internal positive control) monitors PCR performance and sample inhibition. The Ct(IPC) of a sample should be within ±1.0 Ct of the Ct(IPC) of the NCS. The NTC should have Ct ≥ 35 or be undetermined. A significantly higher Ct(IPC) in a sample indicates possible inhibition; consider dilution or purification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173461713077,"sku":"1101110","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1B_DNA_Quantitation_Kit.png?v=1742280232"},{"product_id":"residual-pg13-dna-quantitation-kit-shentek®","title":"Residual PG13 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual PG13 DNA Quantitation Kit is designed to quantify residual PG13 host cell DNA in biopharmaceutical samples at different stages (in-process through final product). The kit uses quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with sensitivity at the femtogram (fg) level. The kit includes a PG13 DNA Control as a reference standard and reagents to prepare standard curves and perform absolute quantitation. For extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide. The kit is supplied for research use only and provides reagents sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (qPCR) using FAM-labeled probe\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to the femtogram (fg) level (analytical sensitivity stated as fg-level in kit documentation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (pg\/μL)\u003c\/td\u003e\r\n \u003ctd\u003e3000 (ST0 stock) and working standards ST1=300, ST2=30, ST3=3, ST4=0.3, ST5=0.03, ST6=0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (demonstrated by standards)\u003c\/td\u003e\r\n \u003ctd\u003eApproximately 300 to 0.003 pg\/μL (prepared standard series provides \u0026gt;5 orders of magnitude coverage from working standards)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePer-reaction reagent volumes (qPCR MIX)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + PG13 Primer\u0026amp;Probe MIX 3 μL (total 20 μL qPCR MIX per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Roche LightCycler 480 II (and other compatible real-time PCR systems supporting FAM detection and absolute quantitation mode)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02 (PG13-DNA detector); Auto baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger than mean Ct of lowest standard (or \u0026lt; QL when QL \u0026lt; lowest standard); verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C storage; kit components stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram (fg) level for detection of trace residual PG13 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA Control and a serial dilution standard curve to perform absolute quantitation (standard curve method).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e qPCR Reaction Buffer and PG13 Primer \u0026amp; Probe mix provided for streamlined plate setup and consistent reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Detailed instructions for serial dilutions, controls (ERC, PCS, NCS, NTC), qPCR mix preparation and recommended analysis parameters.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eResearch use supply:\u003c\/strong\u003e Supplied for Research Use Only (RUO) and includes reagents sufficient for 100 reactions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical process intermediates\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual PG13 DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA assays targeting PG13 cell line\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control monitoring of host cell DNA removal\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer \u0026amp; Probe MIX (NNC101) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C for working use as directed)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards and controls carefully; include at least five concentrations in the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before running the qPCR.\u003c\/p\u003e\r\n \u003cp\u003e• Follow recommended analysis settings (Manual Ct, Threshold 0.02, Auto Baseline) or validate settings per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• QC acceptance: ERC recovery 50%–150%; NTC should be ≥35 cycles or undetermined; NCS Ct must be higher than the lowest standard Ct (or meet QL criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses quantitative PCR (qPCR) with a FAM-labeled probe for absolute quantitation using a standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sensitivity can I expect from the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit documentation reports sensitivity at the femtogram (fg) level. Working standard series are provided down to 0.003 pg\/μL (3 fg\/μL), but laboratories should validate the limit of quantitation (QL) in their own workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reagents be prepared and loaded?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare qPCR MIX (17 μL qPCR Reaction Buffer + 3 μL Primer\u0026amp;Probe MIX per reaction), aliquot 20 μL per well and add 10 μL of sample or standard (total 30 μL reaction). Include NTC, NCS, PCS and ERC controls and run standards in triplicate. Seal plate, mix and centrifuge briefly before starting the run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should be ≥35 cycles or undetermined. NCS Ct should be larger than the mean Ct of the lowest standard or meet QL criteria determined in validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173516992693,"sku":"1101123","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Quantitation_Kit.png?v=1742289744"},{"product_id":"residual-mrc-5-dna-quantitation-kit-shentek®","title":"Residual MRC-5 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MRC-5 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual MRC-5 host cell DNA in biopharmaceutical samples at in-process and final product stages. The kit enables rapid, specific, and reliable absolute quantitation down to the femtogram (fg) level using an included MRC-5 DNA Control as reference standard. The kit is supplied as reagents for 100 reactions (Product No.: 1101124) and is intended for research use only. For sample extraction guidance, refer to the corresponding SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MRC-5 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (FAM reporter)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ analytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level; lowest standard used 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eStandard points: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection value of NTC should be ≤ 6.00 fg\/µL (or laboratory-specific criteria after method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction mix per test\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: qPCR Reaction Buffer 17 µL + MRC-5 Primer \u0026amp; Probe MIX 3 µL (prepare 20 µL qPCR MIX then add 10 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95°C 10:00 (1 cycle); Denaturation: 95°C 0:15 (40 cycles); Annealing\/extension and read: 60°C 1:00 (40 cycles)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 example provided); compatible with standard real-time PCR instruments\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format and size\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions; Product No. 1101124; Version A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (individual components stored at -20 °C as labeled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eResult reporting units\u003c\/td\u003e\r\n \u003ctd\u003eMean Quantity reported in pg\/µL (results exportable from instrument software)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ acceptance\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard; NTC detection value ≤ 6.00 fg\/µL; ERC recovery 50–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to the femtogram level (lowest standard 0.03 pg\/µL = 30 fg\/µL) enabling detection of trace residual host-cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes MRC-5 DNA Control for construction of a standard curve and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe mix simplify setup and reduce preparation variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example settings provided for common real-time PCR platforms (ABI 7500, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Appropriate for in-process and final product testing, and for method validation and routine QC of residual host cell DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MRC-5 host-cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product QC testing for host-cell DNA content\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery assessment (using ERC samples)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 DNA Control (NNA062) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 Primer \u0026amp; Probe MIX (NNC118) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (remaining unused DDB store at 2–8 °C after thaw; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Read Material Safety Data Sheets (MSDS) before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use RNase\/DNase-free consumables and low-retention tips; follow sterile technique.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points; perform method validation to select sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix on microplate shaker and briefly centrifuge before running.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50–150%; NCS Ct should be larger than the mean Ct of the lowest standard; NTC signal ≤ 6.00 fg\/µL (or lab-defined criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to detect residual MRC-5 DNA at the femtogram (fg) level. The lowest standard provided is 0.03 pg\/µL (30 fg\/µL). The NTC acceptance criterion is ≤ 6.00 fg\/µL; final LOD should be established during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are supplied in a single kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supplies reagents for 100 reactions (Product No. 1101124).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What instrument settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings for ABI 7500 (SDS v1.4) are provided: 30 µL reaction volume; Activation 95°C 10:00; 40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C. If using other instruments, adapt thermal profile and settings according to the instrument documentation and validate accordingly.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store kit components and how long are they stable?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C as labeled. The kit components can be stored under appropriate conditions for up to 24 months. After thawing, store unused DNA Dilution Buffer (DDB) at 2–8 °C; if it becomes cloudy, incubate at 37 °C until clear.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175198253237,"sku":"1101124","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MRC-5_DNA_Quantitation_Kit.png?v=1742289936"},{"product_id":"residual-bovine-dna-quantitation-kit-shentek®","title":"Residual Bovine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 b0C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK4 Residual Bovine DNA Quantitation Kit is designed to quantitate residual bovine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The assay provides rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit includes bovine DNA control, primer \u0026amp; probe mix, internal positive control (IPC) mix, qPCR reaction buffer and DNA dilution buffer. Compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500). For sample extraction recommendations refer to the SHENTEK Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). For Research Use Only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1101112\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical principle\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (kit description states fg-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Calibration range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared standards from 3000 pg\/bcl down to 0.03 pg\/bcl (ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 bL total reaction volume (20 bL qPCR MIX + 10 bL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 bL, Bovine primer \u0026amp; probe MIX 2.8 bL, IPC MIX 1.3 bL (total 20 bL); add 10 bL sample or standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG (optional) 25 b0C 10:00; Activation 95 b0C 10:00; 40 cycles of Denaturation 95 b0C 00:15 and Annealing\/Extension 63 b0C 00:40 (fluorescence read during 63 b0C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria (IPC)\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within b10.0 Ct of NCS (if significantly higher, sample inhibition suspected)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction Recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery rate acceptance range: 50% e2 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NCS\/NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NCS should be larger than mean Ct of the lowest standard; NTC Ct should be at least 2 cycles larger than mean Ct of lowest standard (or follow lab-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 settings example provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 b0C, kit components can be stored at appropriate conditions for up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 bL purified test sample or 10 bL standards\/DDB per reaction (per recommended workflow)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram level (fg), enabling detection of trace residual bovine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a standard-curve based absolute quantitation method with serially diluted bovine DNA controls.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control:\u003c\/strong\u003e Includes an IPC mix to monitor PCR inhibition and validate sample results.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR MIX:\u003c\/strong\u003e Pre-formulated reaction buffer and primer\/probe mix minimize setup steps and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (example settings provided for ABI 7500 and SHENTEK-96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual bovine DNA in biomaterials and biological raw materials\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics, bioprocess intermediates and medical device materials\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and lot release testing for products where bovine-derived contaminants must be monitored\u003c\/li\u003e\r\n \u003cli\u003eResearch applications assessing bovine DNA contamination and extraction recovery\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine DNA Control (NNA032) 100 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine Primer \u0026amp; Probe MIX (NNC042) 300 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 bL d7 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL d7 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination (UV-decontaminate surfaces and equipment, 75% ethanol disinfection).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2-8 b0C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light; include appropriate controls (NTC, NCS, ERC) and run at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer becomes cloudy, warm at 37 b0C until clear; store unused DDB at 2-8 b0C.\u003c\/p\u003e\r\n \u003cp\u003e• UNG treatment step is optional and should be included only if UNG enzyme is added to the reaction mix.\u003c\/p\u003e\r\n \u003cp\u003e• Follow IPC and ERC acceptance criteria: IPC Ct within b11.0 of NCS; ERC recovery 50% to 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) or limit of quantitation (LOQ) for the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for fg-level sensitivity. The provided standard curve ranges from 3000 pg\/bcl to 0.03 pg\/bcl; validated LOD\/LOQ should be established by the user in their laboratory during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR instrument settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings are provided for ABI 7500 (SDS v1.4): optional UNG 25 b0C 10:00; activation 95 b0C 10:00; 40 cycles of 95 b0C 00:15 and 63 b0C 00:40 (fluorescence read at 63 b0C). Adjust settings as needed for other platforms and software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the supplied Bovine DNA Control serially in DNA Dilution Buffer to generate standards: ST0 = 30000 pg\/bcl (stock) then ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl. Include at least five concentrations in the curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included in each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), Extraction Reference Control (ERC), and serial standards (ST1-ST6). Run samples and controls in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175224959157,"sku":"1101112","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Bovine_DNA_Quantitation_Kit.png?v=1742290060"},{"product_id":"residual-procine-dna-quantitation-kit-shentek®","title":"Residual Procine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Porcine DNA Quantitation Kit is designed to quantify residual porcine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The kit provides a rapid, specific and reliable absolute quantitation assay with sensitivity down to the femtogram (fg) level. The product is supplied for 100 reactions (Product No. 1101113) and is intended for research use only. For sample extraction instructions, refer to the SHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). The kit includes internal positive control (IPC) reagents and a porcine DNA standard control to build a standard curve for absolute quantitation.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual porcine DNA in biomaterials (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (kit optimized for absolute quantitation down to 0.03 pg\/µL standard point)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared: ST0 30000 pg\/µL (stock), ST1 3000 pg\/µL, ST2 300 pg\/µL, ST3 30 pg\/µL, ST4 3 pg\/µL, ST5 0.3 pg\/µL, ST6 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 µL; Porcine Primer \u0026amp; Probe MIX 2.8 µL; IPC MIX 1.3 µL; (optional UNG enzyme 0.1 U)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eOptional UNG 25 °C 10:00; Activation 95 °C 10:00; 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 00:40 (annealing\/extension, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorophores \/ channels\u003c\/td\u003e\r\n \u003ctd\u003ePorcine-DNA: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for Porcine-DNA and 0.02 for IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of the NCS (negative control) IPC Ct; large shift indicates inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery criteria (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard concentration; NTC should be 2 cycles larger than mean Ct of lowest concentration (or laboratory-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months; key reagents stored at –20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System; other real-time PCR systems supported with appropriate setup\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Optimized qPCR assay capable of femtogram-level detection for low-abundance residual porcine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes porcine DNA control and recommended serial dilutions to generate an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IPC):\u003c\/strong\u003e IPC MIX included to monitor extraction and PCR inhibition for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated AT qPCR Reaction Buffer and Porcine Primer \u0026amp; Probe MIX reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarryover prevention option:\u003c\/strong\u003e Supports optional UNG treatment step when UNG enzyme is included to prevent carryover contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Detailed guidance for serial dilution, plate layout, thermal cycling and result analysis for reproducible assays.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual porcine DNA in biomaterials (e.g., tissue-derived products, implants, scaffolds)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for animal-derived material processing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and extraction recovery assessment using ERC samples\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of porcine DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine DNA Control (NNA031) 100 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 µL × 2\u003c\/span\u003e\u003cstrong\u003e–20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine Primer \u0026amp; Probe MIX (NNC043) 300 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation: User Guide (this manual)\u003c\/span\u003e\u003cstrong\u003eambient\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes by UV irradiation (30 minutes) and 75% ethanol prior to setup to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents at –20 °C; check expiration dates on labels. Protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations when preparing the standard curve; recommended serial dilution series provided (ST1–ST6).\u003c\/p\u003e\r\n \u003cp\u003e• Prepare NTC, NCS and ERC controls as instructed. Test samples and controls in triplicate for reliable results.\u003c\/p\u003e\r\n \u003cp\u003e• If UNG is used, include 25 °C 10:00 UNG step in the thermal program; UNG is optional but recommended to prevent carryover.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC Ct values to assess inhibition; acceptable IPC Ct shift is ±1.0 relative to NCS.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery rate is 50%–150%; if outside this range investigate extraction and inhibition.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively measure residual porcine DNA in biomaterials using qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 µL (20 µL qPCR MIX + 10 µL sample or standard). qPCR MIX per reaction: AT qPCR Reaction Buffer 15.9 µL, Porcine Primer \u0026amp; Probe MIX 2.8 µL, IPC MIX 1.3 µL. Optional UNG 0.1 U per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the provided Porcine DNA Control with DNA Dilution Buffer to produce a serial dilution series: ST0 30000 pg\/µL then ST1 3000, ST2 300, ST3 30, ST4 3, ST5 0.3, ST6 0.03 pg\/µL. Include at least five concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What criteria indicate assay inhibition or failure?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC mean Ct is \u0026gt; ±1.0 Ct from the NCS IPC Ct, the sample may be inhibitory. ERC recovery outside 50%–150% or abnormal NTC\/NCS Ct values also indicate possible issues; troubleshoot extraction, sample dilution, or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175394730165,"sku":"1101113","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dna-Residual_Procine_DNA_Quantitation_Kit.png?v=1742290235"},{"product_id":"car-tcr-transgene-copy-number-quantitation-kit","title":"CAR\/TCR Transgene Copy Number Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eSHENTEK® CAR\/TCR Transgene Copy Number Quantitation Kit is designed to quantify the copy number of target genes in human cell products derived from common HIV-1 lentiviral vectors (e.g., CAR or TCR genes integrated in CAR-T and TCR-T cell genomes). The kit uses a multiplex qPCR assay to detect DNA sequences related to the integration\/expression elements on the transfer plasmid and a Single Copy Gene (SCG) in human cells, enabling calculation of average copies of target gene per cell. The kit includes a company-produced reference standard and an internal positive control (IPC). The assay is intended for rapid, specific, reliable and universal lentiviral vector applications. For Research Use Only.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eProduct number\u003c\/td\u003e\n \u003ctd\u003eSK030221CA100\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReference standard initial concentration\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR\/TCR Control initial copy number: 2.33 × 10^8 copies\/μL\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStandard curve serial dilutions (copies\/μL)\u003c\/td\u003e\n \u003ctd\u003eST1: 2.33 × 10^6; ST2: 2.33 × 10^5; ST3: 2.33 × 10^4; ST4: 2.33 × 10^3; ST5: 2.33 × 10^2\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction volume\u003c\/td\u003e\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; CAR\/TCR Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReporter dyes \/ targets\u003c\/td\u003e\n \u003ctd\u003eCAR\/TCR target: FAM; SCG: CY5; IPC: VIC; ROX as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\n \u003ctd\u003eManual Ct threshold 0.02; Automatic baseline; verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria for IPC\u003c\/td\u003e\n \u003ctd\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; significant increase indicates inhibition\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eRecommended DNA input per well\u003c\/td\u003e\n \u003ctd\u003eRecommended 5 ng to 30 ng total DNA per well (adjust based on preliminary experiments)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCalculation formula\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample types\u003c\/td\u003e\n \u003ctd\u003ePurified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eMultiplex absolute quantitation:\u003c\/strong\u003e Simultaneous detection of CAR\/TCR target, single copy gene (SCG) and IPC in the same reaction for absolute copy number determination using a standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReference standard included:\u003c\/strong\u003e Proprietary reference standard supplied and pre-dilution scheme provided for a 5-point standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated CAR\/TCR Primer \u0026amp; Probe MIX and IPC MIX simplify assay setup and reduce pipetting error.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for use on SHENTEK-96S, ABI 7500, LineGene 9600plus, CFX96 and similar real-time PCR instruments.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eDesigned for lentiviral vector applications:\u003c\/strong\u003e Optimized for quantifying transgene copy numbers from HIV-1 derived lentiviral vectors (CAR\/TCR).\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQuantification of CAR or TCR transgene copy number in CAR-T and TCR-T cell products\u003c\/li\u003e\n \u003cli\u003eLentiviral vector integration assessment\u003c\/li\u003e\n \u003cli\u003ePreclinical and research cell therapy product characterization\u003c\/li\u003e\n \u003cli\u003eQuality control and batch testing for gene-modified cell products (research use)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Primer \u0026amp; Probe MIX (NNC032) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eIPC MIX (NNC066) 150 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C (store remaining DDB at 2–8 °C); -20 °C for long term per kit table\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Control (NNA022) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex absolute quantitation, standard curve)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean environment: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol to avoid contamination.\u003c\/p\u003e\n \u003cp\u003e• Avoid assay inhibitors; monitor IPC Ct — if sample IPC Ct is significantly higher than NCS IPC Ct, sample may be inhibitory.\u003c\/p\u003e\n \u003cp\u003e• NTC and NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/p\u003e\n \u003cp\u003e• Prepare and run triplicates for standards, NTC, NCS and test samples where possible.\u003c\/p\u003e\n \u003cp\u003e• Recommended sample input per well: 5 ng–30 ng total DNA (optimize as needed).\u003c\/p\u003e\n \u003cp\u003e• Store kit components as labeled (-20 °C) and protect from light; kit stable up to 24 months. If DDB is cloudy, heat to 37 °C until clear.\u003c\/p\u003e\n \u003cp\u003e• Seal plates\/tubes, mix and briefly centrifuge before running; follow instrument-specific setup and analysis instructions.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample type and amount should I use for the assay?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse purified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration. The recommended total DNA per well is between 5 ng and 30 ng; adjust based on preliminary experiments.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is transgene copies per cell calculated?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eCalculate CAR or TCR copies per cell using the formula: CAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG), where CAR\/TCR and SCG quantities are measured from the standard curve (copies\/μL).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15 (40 cycles); Annealing\/Extension 60 °C for 01:00 with fluorescence read during this step.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and control results?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; a significantly higher IPC Ct indicates possible inhibition. NTC and NCS Ct values should be larger than the mean Ct of the lowest standard.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181277864117,"sku":"SK030221CA100","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CARTCRTransgeneCopyNumberQuantitationKitSHENTEK.jpg?v=1757316229"},{"product_id":"virus-dna-rna-extraction-kit-shentek®","title":"Virus DNA \u0026 RNA Extraction Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eNucleic acid extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eVarious: 2-8 °C, -20 °C, RT (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Virus DNA \u0026amp; RNA Extraction Kit is a magnetic-particle based kit designed for isolation of viral DNA and RNA from cell samples (up to 10^7 cells) and harvested virus bulk (liquid). The kit supports both manual workflows and automated extraction with the SHENTEK® rHCDpurify system. Two sample workflows are provided: Method 1 (for viruses replicated in host nucleus or unknown viruses) includes an optional nuclease digestion step after lysis to reduce background nucleic acids; Method 2 (for viruses replicated in host cytoplasm) uses lysis followed by centrifugation and extraction of the supernatant. The kit contains protease digestion, binding, wash and elution reagents and magnetic particles to deliver purified nucleic acids suitable for downstream molecular assays (e.g., PCR\/qPCR, sequencing). The kit is supplied for 50 extractions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePack size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number\u003c\/td\u003e\r\n \u003ctd\u003e1506730\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input capacity\u003c\/td\u003e\r\n \u003ctd\u003eUp to 1 × 10^7 cells; maximum sample volume per well up to 500 µL (automated plate format)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eEluate volume\u003c\/td\u003e\r\n \u003ctd\u003e50–100 µL RNase-free H2O (preheated at 70 °C); ensure \u0026gt;40 µL for downstream assay\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\r\n \u003ctd\u003e200 µL binding buffer + 200–400 µL isopropanol + 30 µL magnetic particles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKey incubation temperatures \u0026amp; times\u003c\/td\u003e\r\n \u003ctd\u003eProtease digestion: 55 °C for 30 min; Nuclease digestion (optional): 37 °C for 30 min; Elution: 70 °C for 7 min; Magnetic particles incubation at RT 10 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\r\n \u003ctd\u003eIncubate magnetic particles at RT 10 min and vortex to mix before use; aliquot to avoid repeated temperature shifts\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMaximum sample volume (plate)\u003c\/td\u003e\r\n \u003ctd\u003eMaximum per deep-well plate well: 500 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months (see individual component storage below)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eManual extraction or automated extraction using SHENTEK® rHCDpurify system; downstream compatible with PCR\/qPCR and sequencing workflows\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDual DNA \u0026amp; RNA extraction:\u003c\/strong\u003e Simultaneous isolation of both viral DNA and RNA from the same sample using magnetic-particle chemistry.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTwo-method workflow:\u003c\/strong\u003e Method 1 includes nuclease digestion for nucleus-replicating or unknown viruses to reduce background; Method 2 optimized for cytoplasm-replicating viruses (centrifugation of lysate).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated operation:\u003c\/strong\u003e Validated for manual tube-based workflows and automated high-throughput extraction with the SHENTEK® rHCDpurify system (96-deep well plate layout supported).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle technology:\u003c\/strong\u003e Magnetic beads provide reliable binding, washing and elution for high-quality nucleic acids suitable for downstream molecular assays.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for downstream molecular assays:\u003c\/strong\u003e Eluted nucleic acids are suitable for PCR\/qPCR and sequencing; recommended to perform detection assays the same day for best accuracy.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit reagents stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of viral DNA and RNA from cell culture samples (up to 1×10^7 cells)\u003c\/li\u003e\r\n \u003cli\u003eExtraction of nucleic acids from harvested virus bulk (liquid)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for PCR and qPCR assays\u003c\/li\u003e\r\n \u003cli\u003ePreparation of viral nucleic acids for sequencing or downstream molecular testing\u003c\/li\u003e\r\n \u003cli\u003eProcessing of rcAAV\/rAAV, replication competent lentivirus (RCL), replication competent retrovirus (RCR) and RNA viruses\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (15 mL × 1 bottle)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (10 mL × 1 bottle)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND025)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (5 mL × 1 bottle)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePretreatment buffer (NND002)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (1.25 mL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2–8 °C (750 µL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (500 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePrecipitation solution I (NND003)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (25 µL × 1 tube) — to be diluted 1:99 with RNase-Free H2O before use\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.2 mL × 6 bottles)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003eStore per label (500 µL × 5 tubes) — follow kit label\/insert\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic bead-based nucleic acid extraction (manual or automated with rHCDpurify)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eye protection, masks, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Separate laboratory areas for reagent preparation, sample preparation and amplification to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure ambient temperature ≥ 22 °C at experiment start.\u003c\/p\u003e\r\n \u003cp\u003e• Change gloves regularly and use dedicated protective equipment for different lab areas.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge tubes before opening to avoid aerosols; spin down liquid on tube caps\/walls.\u003c\/p\u003e\r\n \u003cp\u003e• Discard and disinfect liquid waste and used tips according to lab biosafety procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry magnetic particle pellet; over-drying may hinder resuspension during elution.\u003c\/p\u003e\r\n \u003cp\u003e• Place tubes on magnetic stand with pellet oriented toward the magnet and rotate tubes slowly to accelerate bead aggregation.\u003c\/p\u003e\r\n \u003cp\u003e• After extraction, perform DNA\/RNA detection assays preferably the same day for accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• For automated runs with rHCDpurify: perform UV sterilization for at least 15 minutes and wipe interior with 75% ethanol before and after runs; maintain at least 30 min between extractions.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506730.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506730.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Can this kit isolate both viral DNA and RNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit is formulated to extract both viral DNA and RNA from cell samples and harvested virus liquid.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many samples can be processed with one kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe supplied kit contains reagents for 50 extractions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is the kit compatible with automation?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the SHENTEK® rHCDpurify system (96-deep well plate format).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What elution volume and conditions are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL RNase-Free H2O preheated to 70 °C and incubate at 70 °C for 7 minutes; ensure at least ~40 µL final elute for downstream assays.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45181418963125,"sku":"1506730","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SHENTEK_Virus_DNA_RNA_Extraction_Kit.jpg?v=1742346294"},{"product_id":"replication-competent-lentivirus-rcl-quantitation-kit-shentek®","title":"Replication-Competent Lentivirus (RCL) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: 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#c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Replication-Competent Lentivirus (RCL) Quantitation Kit is used for the quantitative detection of replicable lentiviral RCL in cell therapy products and gene therapy products produced with lentiviral vectors, such as virus-producing cell banks, end of production cells, viral vectors and CAR-T cells. This kit is rapid, specific and reliable, and can work in coordination with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit to quantitate the copy number of replicable lentivirus RCL in samples.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent lentivirus (RCL) in cell therapy and gene therapy products (virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eOne-step reverse transcription quantitative PCR (qRT-PCR) with FAM-labeled probe; ROX passive reference recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided standard curve range (recommended)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard dilutions ST2–ST6: 6 × 10^5, 6 × 10^4, 6 × 10^3, 6 × 10^2, 6 × 10^1 copies\/µL (prepared from ST = 6 × 10^8 copies\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqRT-PCR MIX per reaction 10 µL; add 20 µL sample\/standard\/NTC for a total reaction volume of 30 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003e5×RT-qPCR Buffer 6 µL; RT-qPCR Enzyme MIX 1 µL; RCL Primer\u0026amp;Probe MIX 3 µL; total 10 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 0:30 (1 cycle); 40 cycles: 95 °C 0:15 (denature), 60 °C 1:00 (anneal\/extend, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, Applied Biosystems 7500, LightCycler 480 II, CFX96, qTOWER3 G, etc.).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection\/read settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Threshold setting example: 0.02 (manual Ct), automatic baseline.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be \u0026gt;= 35.00 cycles. NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored at -20 °C; stable for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ dynamic range (inferred from standard curve)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard curve points cover at least 6 × 10^5 to 6 × 10^1 copies\/µL (user should perform validation to determine empirical LOD\/LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate wells recommended for each standard, control and test sample.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e20 µL purified sample per 30 µL reaction (per example workflow); samples must be processed\/extracted (e.g., with SHENTEK Virus DNA \u0026amp; RNA Extraction Kit) prior to qRT-PCR.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRapid and specific quantitation:\u003c\/strong\u003e One-step qRT-PCR assay optimized for accurate quantitation of replication-competent lentivirus (RCL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream sample prep.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, buffer and enzyme mixes; standardized control for construction of a quantitative standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh throughput:\u003c\/strong\u003e Reagents provided for up to 100 reactions and plate-based workflows (96-well format) supported.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eEstablished QC criteria:\u003c\/strong\u003e Includes recommended control acceptance criteria (NTC and NCS Ct thresholds) and example standard curve settings for result interpretation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of replication-competent lentivirus (RCL) in virus-producing cell banks\u003c\/li\u003e\r\n \u003cli\u003eTesting end-of-production cells for RCL contamination\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of RCL in viral vector preparations\u003c\/li\u003e\r\n \u003cli\u003eSafety testing of CAR-T cells and other lentiviral-transduced cell therapy products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and release testing workflows for gene and cell therapy manufacturing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCL Control (Part No. NNA044), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCL Primer\u0026amp;Probe MIX (Part No. NNC077), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5×RT-qPCR Buffer (Part No. NNC078), 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRT-qPCR Enzyme MIX (Part No. NNC079), 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (Part No. NND008), 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (Part No. NND010), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; store all components at -20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent RNase contamination: use RNase-free consumables and work areas; thaw reagents at 2–8 °C or on ice.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate benches, micropipettes and tubes for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\r\n \u003cp\u003e• Process Negative Control Sample (NCS) through the same extraction and handling steps as test samples.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard curve points (ST2–ST6) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Verify instrument-specific parameter settings; follow instrument documentation when adapting program parameters.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403441.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403441.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is intended for research use to quantitatively detect replication-competent lentivirus (RCL) in cell therapy and gene therapy related samples (e.g., virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What detection instrument and dye settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96). Use FAM as the reporter dye and ROX as the passive reference (if applicable).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended reaction and cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 30 µL reaction (10 µL qRT-PCR MIX + 20 µL sample). Thermal program: 50 °C 15:00 (RT), 95 °C 0:30 (activation), then 40 cycles of 95 °C 0:15 and 60 °C 1:00 (fluorescence read).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve and what is the dynamic range?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the supplied RCL control and perform serial 10-fold dilutions to generate ST2–ST6. Recommended standard concentrations span 6 × 10^5 to 6 × 10^1 copies\/µL; include at least five points for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181705552053,"sku":"1403441","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Lentivirus_RCL_Quantitation_Kit.jpg?v=1742361933"},{"product_id":"replication-competent-retrovirus-rcr-quantitation-kit-shentek®","title":"Replication-Competent Retrovirus (RCR) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Replication-Competent Retrovirus (RCR) Quantitation Kit is designed for quantitative detection of Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products produced with retrovirus vectors. The kit supports absolute quantitation using a standard-curve qRT-PCR workflow and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream nucleic acid preparation. The kit provides lyophilized RCR control, primer \u0026amp; probe mix, qRT-PCR buffer and enzyme mix, and reagents sufficient for 100 reactions. Intended for rapid, specific and reliable monitoring of RCR in virus-producing cell banks, end-of-production cells, viral vector preparations and CAR-T cells.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by qRT-PCR using a standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended standard curve points\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST2–ST6 (five concentration points) corresponding to 5 × 10^5, 5 × 10^4, 5 × 10^3, 5 × 10^2, 5 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl stock concentration (after reconstitution)\u003c\/td\u003e\r\n \u003ctd\u003eLabelled RCR Control reconstituted to 5 × 10^8 copies\/μL then serially diluted\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSuggested quantitation range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5 × 10^1 – 5 × 10^5 copies\/μL (based on ST6–ST2 standard points)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection \/ quantitation\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified in manual; lowest standard provided is 5 × 10^1 copies\/μL (ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqRT-PCR mix per well 10 μL + sample 20 μL = total 30 μL reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR component volumes (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003e5×RT-qPCR Buffer 6 μL, RT-qPCR Enzyme MIX 1 μL, RCR Primer\u0026amp;Probe MIX 3 μL (total 10 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during 60 °C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96, qTOWER3 G); settings provided for Applied Biosystems 7500 SDS v1.4\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of the lowest standard concentration.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at -20 °C; components stable up to 24 months (check labels for expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for RCR quantitation:\u003c\/strong\u003e Specifically targets Replication-Competent Retrovirus (RCR) in cell and gene therapy products, enabling product safety testing and release control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a serially diluted lyophilized RCR control and a standard-curve qRT-PCR method to provide copy number (copies\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete qRT-PCR reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, 5× RT-qPCR buffer, RT-qPCR enzyme mix, RNase-free water and a lyophilized control; reagents provided for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common real-time PCR platforms:\u003c\/strong\u003e Validated instructions and program settings provided for multiple instruments (e.g., ABI 7500, LightCycler 480 II, CFX96).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRNase-free and protected formulations:\u003c\/strong\u003e RNase-free water and light-protected mixes supplied; some components labeled 'protect from light'.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitative detection of RCR in virus-producing cell banks\u003c\/li\u003e\r\n \u003cli\u003eTesting end-of-production cells for RCR contamination\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of RCR in viral vector preparations\u003c\/li\u003e\r\n \u003cli\u003eScreening CAR‑T or other cell therapy products for RCR\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing during gene\/cell therapy manufacturing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for RCR monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCR Control (lyophilized powder)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCR Primer \u0026amp; Probe MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5× RT-qPCR Buffer\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRT-qPCR Enzyme MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (1.2 mL × 3)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (1 mL × 1)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR (absolute quantitation by standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Work in RNase-free conditions; use RNase-free consumables and reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; store at -20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice. Do not refreeze repeatedly.\u003c\/p\u003e\r\n \u003cp\u003e• Dissolve lyophilized control by adding specified volume of ddH2O and mix thoroughly (vortex\/flick and centrifuge as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and equipment (UV irradiation 30 min and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard curve points (recommended ST2–ST6) and appropriate controls (NTC, NCS).\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35.00 or undetermined; NCS Ct must be larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403442.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403442.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively detect Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products, including virus-producing cell banks, end-of-production cells, viral vectors and CAR‑T cells.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions does the kit support and what is the reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Each reaction is prepared as a 30 μL total volume (10 μL qRT-PCR mix + 20 μL sample or water), with qRT-PCR mix composed of 6 μL 5×RT-qPCR Buffer, 1 μL RT-qPCR Enzyme MIX and 3 μL RCR Primer\u0026amp;Probe MIX per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the quantitation range and lowest standard provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe recommended standard curve uses five points (ST2–ST6) corresponding to 5 × 10^5 to 5 × 10^1 copies\/μL. The lyophilized RCR Control is reconstituted to 5 × 10^8 copies\/μL and serially diluted to prepare standards.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What instrument settings and acceptance criteria should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal program: RT 50 °C 15:00; Activation 95 °C 00:30; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence at 60 °C). Use absolute quantitation (standard curve). NTC Ct should be ≥ 35 or undetermined; NCS Ct should be larger than mean Ct of the lowest standard.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181719576757,"sku":"1403442","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Retrovirus_RCR_Quantitation_Kit.jpg?v=1742362083"},{"product_id":"rcaav-2-n-quantitation-kit-shentek®","title":"rcAAV-2\/N Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® rcAAV Quantitation Kit is a qPCR-based kit for detection and quantitation of replication-competent adeno-associated virus (rcAAV) contamination in recombinant AAV (rAAV) serotypes designated as rAAV-2\/N. The kit is designed to quantify rcAAV-2\/N contamination in rAAV production samples including harvested cell-culture bulk, purified stocks and final products. The kit contains a T\u0026amp;R DNA control for preparation of an absolute quantitation standard curve, separate Target and Reference Primer \u0026amp; Probe mixes, qPCR reaction buffer and supporting reagents. The kit supports standard-curve (absolute) quantitation and is compatible with common qPCR platforms (e.g., ABI 7500, Bio-Rad CFX96, Roche 480, SHENTEK-96S).\u003c\/p\u003e\n\u003cp\u003eImportant: The ITR sequence of the rAAV test sample must match the provided ITR sequence for correct detection. Reagents supplied are sufficient for 2 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent AAV (rcAAV-2\/N) contamination in rAAV-2\/N samples by qPCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions (kit size)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 2 × 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range \/ standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e20 to 2 × 10^6 copies\/µL (standard curve concentrations: ST6=2×10^1 to ST1=2×10^6 copies\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e20 copies\/µL (lowest standard used for quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (10–10.04 µL qPCR mix + 20 µL sample\/standard per well)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling (example ABI 7500)\u003c\/td\u003e\r\n \u003ctd\u003ePre-melt 95 °C 10:00 (1 cycle); Denature 95 °C 00:15; Anneal\/extend 60 °C 00:30; Read at 72 °C 00:30; 40 cycles. (Note: ABI 7500 users set final read step to 72 °C 34 s.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector channels \/ probes\u003c\/td\u003e\r\n \u003ctd\u003eTarget-2 detected in FAM channel; Reference-2 detected in CY5 channel; separate primer\/probe mixes provided\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eReference-2 threshold = 0.06; Target-2 threshold = 0.02; use automatic baseline and manual Ct\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct (Reference and Target) should be \u0026gt;35 or undetermined. NCS Ct values should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eContamination calculation\u003c\/td\u003e\r\n \u003ctd\u003ercAAV contamination rate = (Detection value of Target genes) ÷ (Detection value of Reference genes × dilution factor ÷ 2). Note: each rAAV contains two copies of the Reference gene.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, Bio-Rad CFX96, SHENTEK-96S, Roche 480 and other real-time PCR systems (ROX usage depends on instrument)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for rcAAV-2\/N detection:\u003c\/strong\u003e Specifically optimized to quantify replication-competent AAV contamination in rAAV-2\/N serotypes using ITR-targeted assays.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation by standard curve:\u003c\/strong\u003e Includes T\u0026amp;R DNA Control and dilution scheme to generate a 6-point standard curve (2×10^1 – 2×10^6 copies\/µL) for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete assay components:\u003c\/strong\u003e Provides qPCR reaction buffer, Target and Reference primer\/probe mixes, ROX option, DNA dilution buffer and DNA control for streamlined setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR instruments; ROX addition is optional depending on instrument model.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProcedural controls and guidance:\u003c\/strong\u003e Includes NTC and NCS guidance, recommended plate layouts, Ct thresholds, and sample dilution recommendations to support robust QC workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of rcAAV contamination in recombinant AAV production (rAAV stocks and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing during rAAV manufacturing\u003c\/li\u003e\r\n \u003cli\u003eAnalysis of harvested cell-culture samples for presence of replication-competent AAV\u003c\/li\u003e\r\n \u003cli\u003eLot release and batch testing for rcAAV contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eT\u0026amp;R DNA Control-2 (lyophilized powder, 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ercAAV qPCR Reaction Buffer (400 µL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTarget Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReference Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e100× ROX (20 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (1 mL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e2–8 °C (or -20 °C long-term)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 2 × 100 reactions | Method: qPCR (Absolute Quantitation by Standard Curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, lab coat, eye protection, mask) when handling reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-treat surfaces, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Benzonase nuclease treatment of rAAV stocks prior to extraction is required to remove non-encapsidated nucleic acids and improve direct-qPCR accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit instructions for sample extraction when applicable.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use; protect light-sensitive reagents (reaction buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge the lyophilized DNA control at 12,000 rpm for 1 min and reconstitute precisely with 55 µL ddH2O before preparing dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh (recommended six points) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Add 100× ROX only if required by the qPCR instrument (ABI 7500 requires ROX; SHENTEK-96S and Roche 480 generally do not).\u003c\/p\u003e\r\n \u003cp\u003e• Use recommended Ct threshold settings (Reference = 0.06; Target = 0.02) and check slope\/intercept\/R² of standard curve for assay validity.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403444.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403444.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supports purified rAAV bulk, final product stocks and harvested cell-culture samples. For bulk or final product stocks, perform Benzonase treatment and nucleic acid extraction prior to qPCR. Cell culture samples may be extracted using the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay dynamic range and lowest quantifiable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe standard curve covers 2 × 10^1 to 2 × 10^6 copies\/µL (20 to 2,000,000 copies\/µL). The lowest standard used for quantitation (LOQ) is 20 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How is rcAAV contamination rate calculated?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ercAAV contamination rate = (Measured copies of Target gene) ÷ (Measured copies of Reference gene × dilution factor ÷ 2). Note that each rAAV contains two copies of the Reference gene.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Do I need to add ROX to every instrument?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. Addition of 100× ROX is instrument-dependent. ABI 7500 requires 0.04 µL ROX per reaction for the Target assay; SHENTEK-96S, Roche 480 and some other systems do not require ROX. Follow your instrument documentation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). For Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions x 2","offer_id":45181732618421,"sku":"1403444","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rcAAV-2_N_Quantitation_Kit.jpg?v=1742362203"},{"product_id":"rcaav-5-n-quantitation-kit-shentek®","title":"rcAAV-5\/N Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® rcAAV Quantitation Kit is designed for qPCR detection and absolute quantitation of replication-competent adeno-associated virus (rcAAV) contamination in rAAV-5\/N serotypes. The kit is suitable for testing recombinant AAV bulk, purified end-products and harvested cell-culture samples. The assay targets the ITR sequence of rAAV-5\/N and includes T\u0026amp;R DNA control material, separate Reference and Target primer\/probe mixes, and reagents for construction of a standard curve for absolute quantitation. Benzonase treatment of rAAV prior to extraction is recommended to remove unprotected nucleic acids and improve accuracy of direct qPCR measurements.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (linear range)\u003c\/td\u003e\r\n \u003ctd\u003e20 - 2 × 10^6 copies\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard points (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 2 × 10^6 copies\/µL; ST2 = 2 × 10^5; ST3 = 2 × 10^4; ST4 = 2 × 10^3; ST5 = 2 × 10^2; ST6 = 2 × 10^1\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (10 µL qPCR MIX + 20 µL sample\/standard\/DDB per well)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eReference: 8 µL rcAAV qPCR Reaction Buffer + 2 µL Reference Primer\u0026amp;Probe MIX-5; Target: 8 µL rcAAV qPCR Reaction Buffer + 2 µL Target Primer\u0026amp;Probe MIX-5 (+ 0.04 µL 100×ROX optional for some instruments)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program (example \/ ABI7500)\u003c\/td\u003e\r\n \u003ctd\u003ePre-melt 95 °C 10:00 (1 cycle); Activation 95 °C 0:15; Denature 60 °C 0:30; Anneal\/Extend 72 °C 0:30 (read fluorescence during this step); 40 cycles. (Note: ABI7500 users: change final step timing to 72 °C 34 s.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, Bio-Rad CFX96, SHENTEK-96S, Roche LightCycler 480 (and other real-time PCR instruments with appropriate dye settings)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eReference-5 threshold = 0.06; Target-5 threshold = 0.02; Manual Ct, Automatic baseline\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt (Reference and Target) for NTC should be \u0026gt;35.00 or undetermined. Ct values for NCS should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicates recommended for standards, controls and samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCalculation of rcAAV contamination rate\u003c\/td\u003e\r\n \u003ctd\u003eContamination rate = (Detection value of Target genes) ÷ (Detection value of Reference genes × dilution factor ÷ 2). Note: each rAAV contains two copies of Reference gene.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ercAAV-specific detection:\u003c\/strong\u003e Designed to detect replication-competent AAV contamination (rcAAV) in rAAV-5\/N serotypes by targeting the ITR sequence.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes a T\u0026amp;R DNA control and instructions for preparing a 6-point standard curve enabling absolute quantitation (copies\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Separate Reference and Target Primer\u0026amp;Probe MIX-5 provided to streamline assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (ABI 7500, CFX96, SHENTEK-96S, Roche 480) with guidance for ROX usage per instrument.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuality control guidance:\u003c\/strong\u003e Includes recommended controls (NTC, NCS) and acceptance criteria (Ct thresholds) to support assay validity.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantification of rcAAV contamination in rAAV-5\/N production lots\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of rAAV bulk and purified end-products\u003c\/li\u003e\r\n \u003cli\u003eTesting harvested cell-culture samples for rcAAV following infection\/production\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring during rAAV manufacturing and downstream purification\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eT\u0026amp;R DNA Control-5 (NNA028)\u003c\/span\u003e\u003cstrong\u003e-20 °C (lyophilized)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ercAAV qPCR Reaction Buffer (NNB009) 400 µL × 4 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTarget Primer\u0026amp;Probe MIX-5 (NNC050) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReference Primer\u0026amp;Probe MIX-5 (NNC051) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e100× ROX (NND007) 20 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (NND010) 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 2 × 100 reactions | Method: qPCR (absolute quantitation \/ standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Benzonase nuclease treatment of rAAV stock or final product is recommended prior to nucleic acid extraction to remove unprotected nucleic acids and improve accuracy of direct qPCR.\u003c\/p\u003e\r\n \u003cp\u003e• Work area, pipettes and tubes should be decontaminated (UV 30 min, 75% ethanol) before setup to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge prior to use. Reconstitute lyophilized DNA control with precise volume and ensure complete dissolution (centrifuge and invert as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components under recommended conditions and check expiration date. Unused DDB should be stored at 2–8 °C; if cloudy, warm to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five concentration points in the standard curve; perform triplicates for standards, controls and samples.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure sample dilutions place sample within the standard curve range (20–2 × 10^6 copies\/µL) for Reference gene. Target gene may be tested undiluted for high-titer samples; dilute Reference measurements as needed.\u003c\/p\u003e\r\n \u003cp\u003e• Set instrument-specific ROX usage according to instructions (e.g., ABI7500: add 0.04 µL ROX per reaction; other instruments may not require ROX).\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct values should be \u0026gt;35 or undetermined; NCS Ct must be larger than mean Ct of lowest standard. Multiply measured concentration by sample dilution factors for final copies\/mL reporting.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403445.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403445.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the quantitation range of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe validated standard curve range is 20 to 2 × 10^6 copies\/µL (use ST1–ST6 standards as described).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store the kit and reagents?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures; frozen components are stored at -20 °C. Check individual component labels and the user guide for storage and stability (kit stable for up to 24 months).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be included with each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a no-template control (NTC), a negative quality control (NCS), and a standard curve (minimum five concentrations recommended). Test samples and controls should be run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How is rcAAV contamination rate calculated?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eContamination rate = (Target gene copies) ÷ (Reference gene copies × dilution factor ÷ 2). Note that each rAAV contains two copies of the Reference gene.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions x 2","offer_id":45181756375221,"sku":"1403445","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rcAAV-5_N_Quantitation_Kit.jpg?v=1742362372"},{"product_id":"mycoplasma-orale-sensitivity-standard-10-cfu-shentek™","title":"Mycoplasma Orale Sensitivity Standard (10 CFU) MycoSHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n 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font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strains); -20 °C (DNA Dilution Buffer) (shelf life 6 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycoplasma Sensitivity Standards are non-infectious controls designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT), such as real-time qPCR, for mycoplasma detection. They provide defined mycoplasma target loads to support method validation and replacement of traditional culture-based assays. Prepared by adding the specified volume of sample matrix to the provided standards to produce a test solution (final concentration 10 CFU\/mL when filled to 1 mL). Intended for use with appropriate DNA extraction procedures and compatible MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit. Not intended for culture; research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal concentration per tube\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU per tube (when prepared to 1 mL final volume yields 10 CFU\/mL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended detection sensitivity (regulatory guidance)\u003c\/td\u003e\r\n \u003ctd\u003ePer EP\/USP\/JP guidance, NAT methods should reach 10 CFU\/mL to replace culture methods and 100 CFU\/mL to replace indicator cell culture methods\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMycoplasma strains: -65 °C; DNA Dilution Buffer (DDB): -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e6 months (check expiration date on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample preparation\u003c\/td\u003e\r\n \u003ctd\u003eAdd sample matrix to each tube to 1 mL final volume to achieve 10 CFU\/mL; vortex 10 s; centrifuge 30 min at 16,000 × g, 2–8 °C; carefully remove 500 µL supernatant leaving pellet-containing 500 µL for DNA extraction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended workflow\u003c\/td\u003e\r\n \u003ctd\u003eEquilibrate to room temperature, perform appropriate DNA extraction, then real-time PCR (qPCR)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eHandling warnings\u003c\/td\u003e\r\n \u003ctd\u003eDo not dilute strains; avoid repeated freeze–thaw; do not mix reagents from different batches\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eDesigned to be used with MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDefined low-level sensitivity standards:\u003c\/strong\u003e Each tube contains a defined 10 CFU nominal mycoplasma amount to validate assay sensitivity down to regulatory targets.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious, non-culture standards:\u003c\/strong\u003e Standards are non-infectious and not intended for culture-based assays — optimized for NAT\/qPCR workflows.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use format:\u003c\/strong\u003e Supplied in pre-aliquoted tubes; prepare test solution by adding sample matrix to the labeled volume to obtain the target concentration.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRegulatory-aligned:\u003c\/strong\u003e Prepared to support method validation according to EP\/USP\/JP recommendations for replacing culture methods with NAT.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of nucleic acid amplification tests (qPCR) for mycoplasma detection\u003c\/li\u003e\r\n \u003cli\u003eMethod sensitivity verification during assay development and qualification\u003c\/li\u003e\r\n \u003cli\u003eQuality control and lot release testing workflows for cell therapy and biologics\u003c\/li\u003e\r\n \u003cli\u003eTraining and proficiency testing for laboratory staff performing mycoplasma NAT assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma orale Sensitivity Standard (10 CFU)\u003c\/span\u003e\u003cstrong\u003e-65 °C (10 CFU × 5 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma pneumoniae Sensitivity Standard (10 CFU)\u003c\/span\u003e\u003cstrong\u003e-65 °C (10 CFU × 5 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma hyorhinis Sensitivity Standard (10 CFU)\u003c\/span\u003e\u003cstrong\u003e-65 °C (10 CFU × 5 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 5 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 15 tubes total (3 species × 5 tubes); each tube contains 10 CFU (prepare to 1 mL to yield 10 CFU\/mL) | Method: qPCR (real-time PCR) after DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date; check labels.\u003c\/p\u003e\r\n \u003cp\u003e• Each strain must not be diluted — dilution may cause uneven distribution and risk undetected contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate reagents to room temperature and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control per test sample; include a negative control using the sample matrix to monitor inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• Appropriate sample DNA extraction is strongly recommended before PCR to reduce inhibition and maximize sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• This product is for research use only; not for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate laboratory clothing and disposable gloves for safety.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1501501.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1501501.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the nominal mycoplasma concentration in each tube and how do I prepare the test sample?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eEach tube contains a nominal 10 CFU. To prepare the test solution, add the indicated volume of sample matrix to each tube to bring the final volume to 1 mL (resulting in 10 CFU\/mL). Vortex 10 seconds, centrifuge 30 minutes at 16,000 × g (2–8 °C), carefully remove 500 µL supernatant, and use the remaining 500 µL for DNA extraction followed by qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the standards be stored and what is the shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycoplasma strain tubes at -65 °C and DNA Dilution Buffer (DDB) at -20 °C. The kit shelf life is 6 months — always check the expiration date on the labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can these standards be cultured or are they safe to handle without BSL precautions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe standards are non-infectious and are not intended for culture. They are intended for use as NAT\/qPCR controls. Follow standard laboratory safety practices (gloves, lab coat) when handling.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I include in my PCR assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude at least one negative control for each test sample and a negative control using the sample matrix to monitor for inhibition. Follow your assay validation protocol and manufacturer recommendations for positive and internal controls.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"10 CFU\/tube x 5 tubes","offer_id":45181758734517,"sku":"1501501","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Orale_Sensitivity_Standard_10_CFU.jpg?v=1742363011"},{"product_id":"mycoplasma-pneumoniae-sensitivity-standard-10-cfu-mycoshentek®","title":"Mycoplasma pneumoniae Sensitivity Standard (10 CFU) MycoSHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strains); -20 °C (DDB) (shelf life 6 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThe MycoSHENTEK® Mycoplasma Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT), specifically qPCR\/real-time PCR, for mycoplasma detection. These standards contain defined nominal amounts of mycoplasma (10 CFU per tube) and are intended to be mixed with sample matrix to produce a test solution (e.g., 10 CFU\/mL) for downstream DNA extraction and qPCR detection. The standards support method validation and performance verification in accordance with pharmacopeial guidance (EP\/USP\/JP), where NAT sensitivity targets include 10 CFU\/mL to replace culture methods and 100 CFU\/mL to replace indicator cell culture methods. The product is not for culture and is intended for research use only. Compatible with MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit for complete workflow validation.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal concentration\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU per vial (when reconstituted to 1 mL final volume → 10 CFU\/mL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAvailable strains \/ catalog\u003c\/td\u003e\r\n \u003ctd\u003eMycoplasma orale (Product No. 1501501), Mycoplasma pneumoniae (Product No. 1501503), Mycoplasma hyorhinis (Product No. 1501505)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ quantity\u003c\/td\u003e\r\n \u003ctd\u003eEach strain: 10 CFU × 5 tubes; DNA Dilution Buffer (DDB): 1.5 mL × 5 tubes\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStrains: -65 °C; DNA Dilution Buffer: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e6 months (check expiration date on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePreparation guidance\u003c\/td\u003e\r\n \u003ctd\u003eThaw at 2–8 °C. Add sample matrix to bring tube volume to 1 mL to achieve 10 CFU\/mL. Vortex 10 s and centrifuge 30 min at 16,000 × g, 2–8 °C. Remove 500 µL supernatant carefully and mix remaining 500 µL for DNA extraction.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended analytical sensitivity (pharmacopeial target)\u003c\/td\u003e\r\n \u003ctd\u003ePer EP\/USP\/JP guidance: demonstrate method sensitivity to 10 CFU\/mL to replace culture methods; 100 CFU\/mL to replace indicator cell culture methods\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious reference standards:\u003c\/strong\u003e Standards are non-infectious and not intended for culture—safer and convenient for laboratory use.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDefined nominal CFU:\u003c\/strong\u003e Each vial contains a nominal 10 CFU to enable validation of low-level assay sensitivity.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePrepared for NAT\/qPCR validation:\u003c\/strong\u003e Specifically designed to validate nucleic acid amplification techniques such as qPCR\/real-time PCR according to pharmacopeial guidance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSimple preparation workflow:\u003c\/strong\u003e Add sample matrix to 1 mL, vortex, centrifuge, remove supernatant and extract DNA for downstream qPCR.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with extraction and detection kits:\u003c\/strong\u003e Recommended use with MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit for complete workflow verification.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of qPCR\/NAT mycoplasma detection assays\u003c\/li\u003e\r\n \u003cli\u003eMethod sensitivity verification to meet EP\/USP\/JP requirements\u003c\/li\u003e\r\n \u003cli\u003eQuality control and assay performance checks in cell therapy and biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eWorkflow verification for DNA extraction and qPCR detection kits\u003c\/li\u003e\r\n \u003cli\u003eLaboratory training and proficiency testing for low-level mycoplasma detection\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma orale Sensitivity Standard (10 CFU × 5 tubes) (Product No. 1501501)\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma pneumoniae Sensitivity Standard (10 CFU × 5 tubes) (Product No. 1501503)\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma hyorhinis Sensitivity Standard (10 CFU × 5 tubes) (Product No. 1501505)\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 5 tubes) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable (standards: 10 CFU × 5 tubes per strain) | Method: qPCR (real-time PCR) for downstream detection after DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Do not dilute the strain vials; dilution may cause uneven distribution and undetected contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Perform appropriate sample DNA extraction prior to PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• PCR inhibition may be caused by sample matrix—include a negative control prepared in the same sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control for each test run\/sample set.\u003c\/p\u003e\r\n \u003cp\u003e• This product is intended for research use only and must not be used for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate lab clothing and disposable gloves for safety.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1501503.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1501503.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the MycoSHENTEK® Mycoplasma Sensitivity Standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThese standards are intended for research use only to validate and verify the sensitivity and robustness of NAT\/qPCR mycoplasma detection workflows. They are non-infectious and not for clinical diagnosis or culture.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare a test solution from the standard vial?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the vial at 2–8 °C. Add sample matrix to bring the tube volume to 1 mL to achieve a final concentration of 10 CFU\/mL. Vortex 10 seconds, centrifuge 30 minutes at 16,000 × g (2–8 °C), carefully remove 500 µL supernatant, and mix the remaining 500 µL for DNA extraction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the storage conditions and shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStrain vials should be stored at -65 °C, DNA Dilution Buffer (DDB) at -20 °C. The kit has a shelf life of 6 months—check the expiration date on the label.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can these standards be used for culture or as infectious controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The standards are non-infectious and are not intended for culture; they are designed solely as molecular sensitivity standards for NAT\/qPCR validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Not for clinical diagnosis or treatment. Non-infectious standards; not intended for culture.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"10 CFU\/tube x 5 tubes","offer_id":45181769187509,"sku":"1501503","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Pneumoniae_Sensitivity_Standard_10_CFU.jpg?v=1742363076"},{"product_id":"mycoplasma-hyorhinis-sensitivity-standard-10-cfu-mycoshentek®","title":"Mycoplasma hyorhinis Sensitivity Standard (10 CFU) MycoSHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strain); -20 °C (DNA Dilution Buffer, DDB); shelf life 6 months\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycoplasma Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT), such as qPCR, for detection of mycoplasma contamination. The standards provide defined quantities (10 CFU) of specific Mycoplasma strains for use as spike-in or positive controls to assess assay sensitivity and workflow performance. They are intended to support replacement of traditional culture methods with validated NAT approaches in accordance with pharmacopeial guidance (EP, USP, JP). The standards are not intended for culture. The product is supplied with DNA Dilution Buffer (DDB) to prepare test solutions and is compatible with MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit for downstream extraction and real-time PCR analysis.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (prepared by adding sample matrix to 1 mL to achieve final concentration of 10 CFU\/mL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided concentration per tube\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU per tube (each strain: 10 CFU × 5 tubes)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIncluded strains\u003c\/td\u003e\r\n \u003ctd\u003eMycoplasma orale (NNE006), Mycoplasma pneumoniae (NNE009), Mycoplasma hyorhinis (NNE008)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStrains: -65 °C; DNA Dilution Buffer (DDB, NND001): -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e6 months (check expiration on label)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample preparation volume\u003c\/td\u003e\r\n \u003ctd\u003eAdd sample matrix to each tube to a final volume of 1 mL to reach 10 CFU\/mL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended processing conditions\u003c\/td\u003e\r\n \u003ctd\u003eThaw at 2–8 °C; vortex 10 seconds; centrifuge 30 min at 16,000 × g at 2–8 °C; remove 500 µL supernatant and retain 500 µL for DNA extraction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidation and sensitivity verification of NAT\/qPCR assays for mycoplasma; positive control for assay performance\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRegulatory sensitivity reference\u003c\/td\u003e\r\n \u003ctd\u003eMeets pharmacopeial sensitivity targets referenced in EP\/USP\/JP (EP 2.6.7: 10 CFU\/mL to replace culture methods; 100 CFU\/mL to replace indicator cell culture methods)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInfectivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNon-infectious; not intended for culture\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDefined low-copy mycoplasma standards:\u003c\/strong\u003e Supplied as 10 CFU per tube to permit sensitive limit-of-detection and sensitivity verification of qPCR-based mycoplasma NAT workflows.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious, ready-to-use format:\u003c\/strong\u003e Standards are non-infectious and designed for direct use as spike-in controls after addition of sample matrix—no culture required.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeial alignment:\u003c\/strong\u003e Designed to support validation against EP\/USP\/JP guidance for NAT replacement of culture methods (e.g., EP 2.6.7).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow compatibility:\u003c\/strong\u003e Compatible with MycoSHENTEK® Mycoplasma DNA Extraction Kit and MycoSHENTEK® Mycoplasma DNA Detection Kit for streamlined extraction and qPCR analysis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStable packaged format:\u003c\/strong\u003e Supplied in multiple tubes (5 tubes per strain) with DNA Dilution Buffer for reproducible preparation of test solutions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of NAT\/qPCR mycoplasma detection assays\u003c\/li\u003e\r\n \u003cli\u003eLimit-of-detection (LOD) verification and sensitivity qualification\u003c\/li\u003e\r\n \u003cli\u003ePositive control \/ spike-in for mycoplasma testing in cell therapy and biologics workflows\u003c\/li\u003e\r\n \u003cli\u003eAssay development and performance monitoring\u003c\/li\u003e\r\n \u003cli\u003eProficiency testing and laboratory quality control\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma orale Sensitivity Standard (10 CFU) — Part No. NNE006\u003c\/span\u003e\u003cstrong\u003e-65 °C; 10 CFU × 5 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma pneumoniae Sensitivity Standard (10 CFU) — Part No. NNE009\u003c\/span\u003e\u003cstrong\u003e-65 °C; 10 CFU × 5 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycoplasma hyorhinis Sensitivity Standard (10 CFU) — Part No. NNE008\u003c\/span\u003e\u003cstrong\u003e-65 °C; 10 CFU × 5 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) — Part No. NND001\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 5 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 5 tubes per strain; three strains provided (total 15 sensitivity standard tubes). Each tube prepared to 1 mL yields one 10 CFU\/mL test solution. | Method: qPCR (nucleic acid amplification technique, NAT)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date; kit shelf life is 6 months.\u003c\/p\u003e\r\n \u003cp\u003e• Each strain must not be diluted prior to preparing the 1 mL test solution; dilution may cause uneven distribution and risk of undetected contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Use only after equilibrating to room temperature and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Appropriate sample DNA extraction is strongly recommended prior to PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control using the same sample matrix for each test sample; PCR inhibition may be caused by the sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• This product is intended for research use only and should not be used for clinical diagnosis or treatment; follow appropriate laboratory safety practices (lab coat, disposable gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Any operational deviations from the user guide may affect results.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1501505.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1501505.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the MycoSHENTEK® Mycoplasma Sensitivity Standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThey are intended as non-infectious reference standards to validate and verify the sensitivity and robustness of NAT\/qPCR mycoplasma detection workflows, and as positive controls or spike-in materials for assay performance assessment.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare a test solution at 10 CFU\/mL?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the strain at 2–8 °C. Based on the volume indicated on the tube label, add appropriate sample matrix to each tube to a final volume of 1 mL to achieve 10 CFU\/mL. Vortex 10 seconds, centrifuge 30 minutes at 16,000 × g at 2–8 °C, carefully remove 500 µL supernatant (avoid disturbing pellet), and use the remaining 500 µL for DNA extraction and subsequent qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the standards and buffer be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycoplasma strain tubes at -65 °C and DNA Dilution Buffer (DDB, NND001) at -20 °C. Do not repeat freeze–thaw cycles and use within the kit shelf life (6 months).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can these standards be used for culture-based mycoplasma testing?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The standards are non-infectious and not intended for culture. They are designed for NAT\/qPCR validation and verification only.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Non-infectious standards; not for clinical diagnosis or treatment; not for culture.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"10 CFU\/tube x 5 tubes","offer_id":45181780951221,"sku":"1501505","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Hyorhinis_Sensitivity_Standard_10_CFU.jpg?v=1742363179"},{"product_id":"mycoplasma-orale-dna-standard-1-10-copies-µl-mycoshentek®","title":"Mycoplasma orale DNA Standard (1x10⁸ copies\/µL) MycoSHENTEK®","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMycoplasma Orale DNA Standard serves as a positive control for Mycoplasma detection assay.\u003c\/p\u003e\n\u003cp\u003eThe MycoSHENTEK® Mycoplasma orale DNA Standard (1×108 copies\/μL) is purified genomic DNA used as a positive and sensitivity control for PCR assay.\u003c\/p\u003e\n\u003cp\u003eFor extraction and detection information, please refer to the MycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) User Guide (Product No. 1509840) and MycoSHENTEK® Mycoplasma DNA Detection Kit (2G) User Guide (Product No. 1509841).\u003c\/p\u003e\n\u003cp\u003eSize: 100 μL x 1 tube\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-extraction-kit-2g-mycoshentek%E2%84%A2?variant=45191334133941\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-detection-kit-2g-mycoshentek%E2%84%A2?variant=45191337345205\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cdiv style=\"background-color: #fffbeb; border: 1px solid #fde68a; border-radius: 8px; padding: 20px; margin-top: 30px; margin-bottom: 30px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: center; gap: 8px; margin-bottom: 12px;\"\u003e\r\n \u003csvg style=\"width: 20px; height: 20px; color: #d97706; flex-shrink: 0;\" fill=\"currentColor\" viewbox=\"0 0 20 20\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 style=\"font-weight: bold; color: #78350f; margin: 0; font-size: 16px;\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"margin-left: 28px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px; margin-bottom: 10px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n \u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 μL x 1 tube","offer_id":45186764341429,"sku":"1502550","price":1030.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Orale_DNA_Standard_1_10_copies_L.jpg?v=1742363234"},{"product_id":"mycoplasma-pneumoniae-dna-standard-1-10-copies-µl-mycoshentek®","title":"Mycoplasma Pneumoniae DNA Standard (1x10‚Å∏ copies\/µL) MycoSHENTEK®","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMycoplasma Pneumoniae DNA Standard serves as a positive control for Mycoplasma detection assay.\u003c\/p\u003e\n\u003cp\u003eThe MycoSHENTEK® Mycoplasma pneumoniae DNA Standard (1×10⁸ copies\/μL) is purified genomic DNA used as a positive and sensitivity control for PCR assay.\u003c\/p\u003e\n\u003cp\u003eFor extraction and detection information, please refer to the MycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) User Guide (Product No. 1509840) and MycoSHENTEK® Mycoplasma DNA Detection Kit (2G) User Guide (Product No. 1509841).\u003c\/p\u003e\n\u003cp\u003eSize: 100 μL x 1 tube\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-extraction-kit-2g-mycoshentek%E2%84%A2?variant=43795724009653\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-detection-kit-2g-mycoshentek%E2%84%A2\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cdiv style=\"background-color: #fffbeb; border: 1px solid #fde68a; border-radius: 8px; padding: 20px; margin-top: 30px; margin-bottom: 30px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: center; gap: 8px; margin-bottom: 12px;\"\u003e\r\n \u003csvg style=\"width: 20px; height: 20px; color: #d97706; flex-shrink: 0;\" fill=\"currentColor\" viewbox=\"0 0 20 20\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 style=\"font-weight: bold; color: #78350f; margin: 0; font-size: 16px;\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"margin-left: 28px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px; margin-bottom: 10px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n \u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 μL x 1 tube","offer_id":45186781774005,"sku":"1502551","price":1030.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Pneumoniae_DNA_Standard_1_10_copies_L.jpg?v=1742363302"},{"product_id":"mycoplasma-hyorhinis-dna-standard-1-10-copies-µl","title":"Mycoplasma Hyorhinis DNA Standard (1x10‚Å∏ copies\/µL) MycoSHENTEK™","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMycoplasma Hyorhinis DNA Standard serve as a positive control for Mycoplasma detection assay.\u003c\/p\u003e\n\u003cp\u003eThe MycoSHENTEK® Mycoplasma hyorhinis DNA Standard (1×10⁸ copies\/μL) is purified genomic DNA used as a positive and sensitivity control for PCR assay.\u003c\/p\u003e\n\u003cp\u003eFor extraction and detection information, please refer to the MycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) User Guide (Product No. 1509840) and MycoSHENTEK® Mycoplasma DNA Detection Kit (2G) User Guide (Product No. 1509841).\u003c\/p\u003e\n\u003cp\u003eSize: 100 μL x 1 tube\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-extraction-kit-2g-mycoshentek%E2%84%A2?variant=43795724009653\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G)\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/mycoplasma-dna-detection-kit-2g-mycoshentek%E2%84%A2?variant=43795742916789\"\u003e\u003cstrong\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\u003cdiv style=\"background-color: #fffbeb; border: 1px solid #fde68a; border-radius: 8px; padding: 20px; margin-top: 30px; margin-bottom: 30px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: center; gap: 8px; margin-bottom: 12px;\"\u003e\r\n \u003csvg style=\"width: 20px; height: 20px; color: #d97706; flex-shrink: 0;\" fill=\"currentColor\" viewbox=\"0 0 20 20\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 style=\"font-weight: bold; color: #78350f; margin: 0; font-size: 16px;\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"margin-left: 28px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px; margin-bottom: 10px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n \u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 μL x 1 tube","offer_id":45186789736629,"sku":"1502552","price":1030.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Hyorhinis_DNA_Standard_1_10_copies_L.jpg?v=1742363409"},{"product_id":"mycobacterium-phlei-sensitivity-standard-10-cfu-mycoshentek™","title":"Mycobacterium Phlei Sensitivity Standard (10 CFU) MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strain); -20 °C (DDB) (stable for 12 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycobacterium Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT) for detection of mycobacteria. Available as defined loads (10 CFU or 100 CFU per tube), the standards are not intended for culture and are used by adding a specified volume of sample matrix to each tube to prepare test solutions for downstream DNA extraction and real-time PCR (qPCR) analysis. These standards support method validation and routine QC for mycobacterial DNA extraction and qPCR assays and are aligned with recommendations from the European Pharmacopoeia (EP), United States Pharmacopoeia (USP) and Chinese Pharmacopoeia (ChP). The product is supplied with a DNA Dilution Buffer (DDB) for preparation of test solutions. Appropriate sample DNA extraction prior to PCR is recommended to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal load per tube\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU or 100 CFU per tube (provided as separate catalogue items)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct formats \/ pack size\u003c\/td\u003e\r\n \u003ctd\u003eEach format supplied as 3 tubes per pack: 10 CFU × 3 tubes (NNE002) or 100 CFU × 3 tubes (NNE001); DNA Dilution Buffer (NND001) 1.5 mL × 3 tubes\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReference standard for validation and sensitivity assessment of NAT\/qPCR assays for mycobacteria; not for culture, not infectious\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage temperatures\u003c\/td\u003e\r\n \u003ctd\u003eMycobacterium strains: -65 °C; DNA Dilution Buffer (DDB): -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eStable for 12 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended preparation volume\u003c\/td\u003e\r\n \u003ctd\u003eFor 10 CFU tubes, supplement sample matrix to a total volume of 400 µL per tube (follow label instructions for other formats)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eDesigned for use with MycoSHENTEK® Mycobacteria DNA Extraction Kit and MycoSHENTEK® Mycobacteria DNA Detection Kit and other validated qPCR workflows\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSafety \/ infectivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNon-infectious material; not intended for culture\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWarnings on dilution\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eEach strain must not be diluted — dilution may cause uneven distribution and risk of undetected contamination\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDefined CFU reference standards:\u003c\/strong\u003e Provided at fixed nominal loads (10 CFU and 100 CFU per tube) for sensitivity assessment and limit-of-detection verification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious, culture-free:\u003c\/strong\u003e Standards are non-infectious and not intended for culture, reducing biosafety concerns while enabling NAT validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-compatible:\u003c\/strong\u003e Optimized for downstream DNA extraction and qPCR-based detection workflows; suitable as positive controls and sensitivity standards.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRegulatory-aligned:\u003c\/strong\u003e Supports validation approaches recommended by EP, USP and ChP to replace lengthy culture-based methods.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConvenient format:\u003c\/strong\u003e Supplied as multiple tubes with matching DNA Dilution Buffer to simplify test solution preparation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of NAT \/ qPCR methods for mycobacteria detection\u003c\/li\u003e\r\n \u003cli\u003eLimit of detection (LOD) and sensitivity verification of qPCR assays\u003c\/li\u003e\r\n \u003cli\u003eRoutine quality control and proficiency testing of mycobacterial molecular assays\u003c\/li\u003e\r\n \u003cli\u003eMethod transfer and regulatory method validation in accordance with EP\/USP\/ChP recommendations\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei Sensitivity Standard (10 CFU) (NNE002) — 10 CFU × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei Sensitivity Standard (100 CFU) (NNE001) — 100 CFU × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 3 tubes per concentration format (each tube contains the stated CFU); use one tube per control\/test as required | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Each strain must not be diluted; dilution may cause uneven distribution and risk of undetected contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate reagents to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate DNA extraction prior to PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control (matrix negative control) for each test; sample matrix may cause PCR inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• This product is intended for research use only and must not be used for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• For safety, use appropriate laboratory PPE (lab coat, disposable gloves) and follow biosafety cabinet procedures when handling.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503603.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503603.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of MycoSHENTEK Mycobacterium Sensitivity Standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThey are non-infectious reference standards intended to validate NAT\/qPCR methods, assess assay sensitivity and serve as positive controls; they are not for culture or clinical diagnosis.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the standards be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycobacterial strain tubes at -65 °C and the DNA Dilution Buffer (DDB) at -20 °C. The kit shelf life is 12 months; check the expiration date on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I dilute the strain tubes to prepare lower concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. Each strain must not be diluted because dilution may cause uneven distribution of mycobacteria and risk undetected contamination. Instead use the provided nominal formats or validated dilution procedures if supported by your internal validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Do I need to extract DNA before qPCR?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. Appropriate sample DNA extraction is strongly recommended before PCR to reduce inhibition and maximize assay sensitivity. Follow the instructions of your DNA extraction kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"10 CFU\/tube x 3 tubes","offer_id":45186793537717,"sku":"1503603","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacterium_Phlei_Sensitivity_Standard_100_CFU.jpg?v=1742263201"},{"product_id":"10-species-cell-line-authentification-kit-shentek®","title":"10-Species Cell Line Authentification Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003ePCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe SHENTEK 10‑Species Cell Line Authentication Kit is a multiplex PCR‑based assay for rapid species verification and cross‑contamination detection of commonly used cell lines in biologics manufacturing. The kit targets mitochondrial genes (Cytochrome Oxidase I (COI), Cytochrome B (CytB), and Cytochrome Oxidase II (COII)) using species‑specific primers that generate distinct amplicon sizes for ten species in a single reaction. Results are analyzed by horizontal agarose gel electrophoresis; species identification and mixed‑species contamination are inferred from the number and sizes of amplicons. The assay has been validated to detect contamination down to ≤1% and includes reagents sufficient for 50 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Compliance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO); for cell line species identification and contamination detection\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection limit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated to detect cross‑contamination at ≤1% (by proportion of species in a mixed sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget genes\u003c\/td\u003e\r\n \u003ctd\u003eMitochondrial genes: Cytochrome Oxidase I (COI), Cytochrome B (CytB), Cytochrome Oxidase II (COII)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpecies detected (amplicon sizes)\u003c\/td\u003e\r\n \u003ctd\u003eBos taurus (Cow) 93 bp; Mus musculus (Mouse) 147 bp; Canis familiaris (Dog) 172 bp; Rattus norvegicus (Rat) 200 bp; Cercopithecus aethiops (African green monkey) 256 bp; Macaca mulatta (Rhesus monkey) 287 bp; Cricetulus griseus (Chinese hamster) 315 bp; Felis catus (Cat) 355 bp; Homo sapiens (Human) 394 bp; Sus scrofa (Pig) 464 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003eRecommended starting material: 5×10^5 freshly cultured cells per extraction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample extraction conditions\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100 µL Cell Extraction Buffer; incubate 60 °C for 1 hour, then 95 °C for 10 minutes; extracts may be stored at -20 °C for up to 1 month\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format and volumes\u003c\/td\u003e\r\n \u003ctd\u003e25 µL total reaction: 17 µL PCR MIX (12.5 µL 2× Multiplex PCR Buffer + 4.5 µL DNA Sizing Primers MIX) + 8 µL template (extract\/controls)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions (as supplied)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003e1) 95 °C 5 min (1 cycle); 2) [95 °C 30 s; 62 °C 3 min; 68 °C 30 s] for 25–30 cycles (28 cycles recommended for pilot study); 3) 68 °C 30 min; 4) 4 °C hold\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAgarose electrophoresis conditions\u003c\/td\u003e\r\n \u003ctd\u003eUse 2% agarose gel in 1× TBE; run at constant 80–110 V until dye front ~60 mm; recommended loading 6–10 µL per well; include DL500 DNA marker\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNA Sizing Ladder\u003c\/td\u003e\r\n \u003ctd\u003eLane 11 (DNA sizing ladder) contains 10 distinguishable bands corresponding to the 10 species; ensure clear visualization for reliable species calling\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage of kit components\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (individual reagent storage listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended replicates\u003c\/td\u003e\r\n \u003ctd\u003eAnalyze each test sample in two independent replicates to ensure reliability\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex PCR for simultaneous detection:\u003c\/strong\u003e Detects and differentiates 10 species in a single PCR reaction using species‑specific primers that produce distinct amplicon sizes.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMitochondrial gene targets:\u003c\/strong\u003e Targets mitochondrial genes (COI, CytB, COII) for robust species discrimination across common cell lines.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity for contamination detection:\u003c\/strong\u003e Validated to detect contaminating species present at or below 1% of the sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eGel‑based readout:\u003c\/strong\u003e Simple analysis by horizontal agarose electrophoresis — no sequencing required; identification based on band size and pattern.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized workflow:\u003c\/strong\u003e Includes cell extraction buffer and pre‑mixed primer and template reagents; protocol provides recommended PCR cycling and gel conditions for reproducible results.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eCell line species verification\u003c\/li\u003e\r\n \u003cli\u003eCross‑contamination detection in cell cultures\u003c\/li\u003e\r\n \u003cli\u003eQuality control for biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eResearch use for cell authentication in academic and industrial labs\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Sizing Templates MIX (NNA048) 25 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCell Extraction Buffer (NNB015) 1.25 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003eStore as labeled; kit components stable up to 24 months\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e2× Multiplex PCR Buffer (NNB016) 320 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eStore as labeled; kit components stable up to 24 months\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Sizing Primers MIX (NNC082) 120 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eStore as labeled; kit components stable up to 24 months\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDEPC‑treated H2O (NND052) 1 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eStore as labeled; kit components stable up to 24 months\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDL500 DNA Marker, 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at 2–8 °C after thawing; aliquot to avoid repeated freeze–thaw\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eComplementary reagents (sold separately): 6× Loading Buffer (Product No. 1801942) and 10× TBE Buffer (Product No. 1801943)\u003c\/span\u003e\u003cstrong\u003eRefer to individual product labels\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: Multiplex PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; use appropriate PPE (gloves, mask, eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Avoid cross‑contamination: use physically separated areas for sample preparation, reagent setup (negative area), positive control handling, and amplification; if separate rooms are not available, clearly demarcate areas.\u003c\/p\u003e\r\n \u003cp\u003e• Perform a pilot study to verify PCR program settings and electrophoresis conditions before routine use.\u003c\/p\u003e\r\n \u003cp\u003e• Open PCR tubes\/caps only in the electrophoresis area; reseal opened amplification products if not immediately loaded to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid repeated freeze–thaw cycles of reagents and extracts; DNA extracts may be stored at -20 °C for up to 1 month.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: No Template Control (NTC), Negative Control Sample (NCS), and DNA sizing ladder; NTC and NCS should show no amplification.\u003c\/p\u003e\r\n \u003cp\u003e• Analyze each test sample in two independent replicates to ensure reliability.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1801940.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1801940.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum proportion of contaminating species that can be detected?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has been validated to detect contaminating species at or below 1% of the total sample.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample is required for extraction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe recommended starting material is 5×10^5 freshly cultured cells per extraction; follow the extraction protocol (100 µL Cell Extraction Buffer, 60 °C for 1 hour, 95 °C for 10 minutes).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are species identified from the gel?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSpecies are identified by comparing amplicon band sizes to the DNA sizing ladder. Distinct band sizes (provided in the kit documentation) correspond to specific species; multiple bands indicate mixed‑species contamination.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How many reactions are included in the kit and what is the reaction format?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 50 reactions. Standard reaction volume is 25 µL (17 µL PCR MIX + 8 µL template).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"25 Reactions × 2","offer_id":45186794127541,"sku":"1801940","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10-species-cell-line-authentification-kit.jpg?v=1742262356"},{"product_id":"mycobacterium-phlei-sensitivity-standard-100-cfu-mycoshentek™","title":"Mycobacterium Phlei Sensitivity Standard (100 CFU) MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strain storage); -20 °C (DNA Dilution Buffer), shelf life 12 months\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycobacterium Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT), specifically qPCR, for detection of Mycobacterium. Available as defined nominal concentrations (10 CFU\/tube and 100 CFU\/tube), the standards are not intended for culture. To prepare test samples, add the specified volume of sample matrix to the standards to reach the desired final concentration (for 10 CFU tubes supplement to a total volume of 400 µL), then perform DNA extraction and real-time PCR. The product is intended as a positive control\/validation standard and is compatible with the MycoSHENTEK® Mycobacteria DNA Extraction Kit and MycoSHENTEK® Mycobacteria DNA Detection Kit. Compliant with recommendations from EP, USP and ChP for replacing culture methods with validated NAT.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal concentration (format)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU per tube (NNE002) and 100 CFU per tube (NNE001)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTube count per kit\u003c\/td\u003e\r\n \u003ctd\u003e3 tubes of mycobacterium standard + 3 tubes DNA Dilution Buffer per kit\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNA Dilution Buffer (DDB)\u003c\/td\u003e\r\n \u003ctd\u003e1.5 mL × 3 tubes (NND001)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage temperature\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStrains: -65 °C; DNA Dilution Buffer: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e12 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidation of NAT\/qPCR sensitivity and robustness; positive control standards (non-infectious)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePreparation guidance\u003c\/td\u003e\r\n \u003ctd\u003eThaw at 2-8 °C; add specified sample matrix volume (for 10 CFU tubes supplement to 400 µL), vortex 10 s, centrifuge briefly, extract DNA, perform qPCR\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRestriction\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNot intended for culture; do not dilute strain tubes\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use sensitivity standards:\u003c\/strong\u003e Provided at defined nominal concentrations (10 CFU and 100 CFU) to support assay sensitivity verification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious materials:\u003c\/strong\u003e Standards are non-infectious and supplied for NAT\/qPCR validation without culture handling risk.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeia-aligned:\u003c\/strong\u003e Designed to support validated NAT approaches recommended by EP, USP and ChP as alternatives to lengthy culture methods.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with MycoSHENTEK kits:\u003c\/strong\u003e Validated workflow includes MycoSHENTEK Mycobacteria DNA Extraction Kit and Mycobacteria DNA Detection Kit for full assay validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConvenient format:\u003c\/strong\u003e Supplied in multiple tubes with accompanying DNA dilution buffer for sample preparation and reproducible use.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of NAT\/qPCR methods for Mycobacterium detection\u003c\/li\u003e\r\n \u003cli\u003eAssay sensitivity verification and limit-of-detection checks\u003c\/li\u003e\r\n \u003cli\u003eMethod qualification and performance verification per EP\/USP\/ChP\u003c\/li\u003e\r\n \u003cli\u003eQuality control and proficiency testing in R\u0026amp;D and QC laboratories\u003c\/li\u003e\r\n \u003cli\u003eOperator training for sample processing, extraction and qPCR workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei (10 CFU) NNE002\u003c\/span\u003e\u003cstrong\u003e-65 °C (store strain tubes frozen)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) NND001 — 1.5 mL × 3 tubes (supplied with 10 CFU kit)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei (100 CFU) NNE001\u003c\/span\u003e\u003cstrong\u003e-65 °C (store strain tubes frozen)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) NND001 — 1.5 mL × 3 tubes (supplied with 100 CFU kit)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable — supplied as positive control standards (3 tubes per concentration) | Method: qPCR (NAT)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Each strain tube must not be diluted; dilution causes uneven distribution and may create undetected contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate reagents to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate sample DNA extraction before PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date; check label expiration.\u003c\/p\u003e\r\n \u003cp\u003e• Operational deviations from the user guide may affect results.\u003c\/p\u003e\r\n \u003cp\u003e• PCR inhibition may be caused by the sample matrix; include a negative control sample using the sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control for each test run.\u003c\/p\u003e\r\n \u003cp\u003e• Intended for research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate laboratory clothing and disposable gloves for safety.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503604.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503604.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What are the available concentrations of the MycoSHENTEK sensitivity standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are provided as Mycobacterium phlei at nominal concentrations of 10 CFU per tube and 100 CFU per tube, each supplied as 3 tubes per kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the standards be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycobacterium strain tubes at -65 °C. Store DNA Dilution Buffer (DDB) at -20 °C. The kit shelf life is 12 months; check the expiration date on the label.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can these standards be used for culture?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. These standards are non-infectious and not intended for culture; they are supplied for NAT\/qPCR validation only.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included when using these standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude at least one negative control with the same sample matrix for each test. Also follow DNA extraction and qPCR kit recommendations to monitor inhibition and assay performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical diagnostic or therapeutic use\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 CFU\/tube x 3 tubes","offer_id":45186799599797,"sku":"1503604","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycobacterium_phlei_Sensitivity_Standard_100_CFU.jpg?v=1742363473"},{"product_id":"telomerase-assay-kit-shentek®","title":"Telomerase Assay kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 18 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Telomerase Assay Kit uses a Real-time Quantitative TRAP method (RQ-TRAP) implemented as a dual-fluorescence qPCR system. The assay includes an internal reference gene (IC, CY5) to control for inhibitors and false negatives, and uses TSR8 as a quantitative reference standard for accurate telomerase activity quantification (TPG Units). The kit is provided as a closed-cap qPCR assay (no gel electrophoresis or ELISA required) to improve throughput and reduce contamination risk. The kit includes reagents for cell lysis, RNase inhibition, TRAP qPCR reagents, TSR8 standard and telomerase positive cell controls and is designed for measuring telomerase activity in cell extracts.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDual-fluorescence real-time quantitative TRAP (RQ-TRAP) qPCR\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 200 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e25 μL total per reaction (23 μL qPCR MIX + 2 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTemplate volume per reaction\u003c\/td\u003e\r\n \u003ctd\u003e2 μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX per reaction\u003c\/td\u003e\r\n \u003ctd\u003e2× TRAP qPCR Reaction Buffer 12.5 μL; TRAP qPCR Reaction Enzyme 1 μL; TRAP Primer\u0026amp;Control MIX 3 μL; TRAP qPCR Reaction Replenisher 6.5 μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR program (ABI7500 example)\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 30 °C 30:00 (1 cycle); Activation: 95 °C 02:00 (1 cycle); 35 cycles of 95 °C 00:15 (denaturation), 50 °C 01:00 (anneal), 68 °C 00:30 (extension, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve (TSR8)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1: 20 amol\/μL (40000 TPG Units); ST2: 2 amol\/μL (4000 TPG Units); ST3: 0.2 amol\/μL (400 TPG Units); ST4: 0.02 amol\/μL (40 TPG Units); ST5: 0.002 amol\/μL (4 TPG Units); ST6: 0.0002 amol\/μL (0.4 TPG Units). Note: ST0 = 200 amol\/μL (400000 TPG Units). 1 amol TSR8 = 1000 TPG Units.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic \/ linear range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.4 to 400,000 TPG Units (based on ST6 - ST0 standard series)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria for standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAmplification efficiency 83.3%–110%; R² ≥ 0.990\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection limit (lowest standard)\u003c\/td\u003e\r\n \u003ctd\u003e0.4 TPG Units (corresponding to 0.0002 amol\/μL TSR8, ST6) — use instrument and software settings to confirm detection limit in your system\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input recommendations\u003c\/td\u003e\r\n \u003ctd\u003eStart from freshly harvested 10^6 cells (viability ≥80%) for extraction; for high-activity samples a 10-fold dilution or extraction from 10^5 cells recommended; for low-activity samples test undiluted extracts. Heat-inactivated extract (85 °C, 10 min) is required as negative control.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTelomerase positive control\u003c\/td\u003e\r\n \u003ctd\u003eTelomerase positive cells Pellet (NNA060) supplied; recommended processing described in protocol\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability (kit)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 18 months; recommended storage –20 °C (short-term below –30 °C acceptable per USP \u0026lt;659\u0026gt;)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility (examples)\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, SHENTEK-96S, LightCycler 480 II (others may be used with appropriate programming\/software adjustments)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDual-fluorescence qPCR (RQ-TRAP):\u003c\/strong\u003e Combines telomeric repeat amplification (TRAP) with real-time dual-channel detection (FAM for telomerase product, CY5 for internal control) for quantitative measurement and inhibition monitoring.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal reference (IC):\u003c\/strong\u003e Internal reference gene (CY5) included to detect sample inhibition and reduce false negatives.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuantitative reference standard (TSR8):\u003c\/strong\u003e TSR8 ssDNA standard with defined amol\/μL to convert qPCR signal to TPG Units for absolute quantification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eClosed-cap workflow:\u003c\/strong\u003e No post-PCR gel electrophoresis or ELISA steps required, reducing hands-on time and contamination risk.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity and wide dynamic range:\u003c\/strong\u003e Standard curve covers 0.4 to 400,000 TPG Units (ST6–ST0), enabling detection from very low to high telomerase activities.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitative measurement of telomerase activity in cultured cells\u003c\/li\u003e\r\n \u003cli\u003eComparative telomerase activity analysis in tumor cell lines, immortalized cells and stem cells (e.g., hiPSCs)\u003c\/li\u003e\r\n \u003cli\u003eTelomerase research in primary cells and mesenchymal stem cells (hMSCs)\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation of telomerase activity assays in research settings\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCell Lysis Buffer (NND058) — 1.5 mL × 5 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase inhibitor (NND060) — 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTelomerase positive cells Pellet (NNA060) — 10^5 cells × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e2× TRAP qPCR Reaction Buffer (NNB022) — 650 μL × 4 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTRAP qPCR Reaction Enzyme (NNC108) — 100 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTSR8 (NNA059) — 50 μL × 1 tube (ssDNA quantitative reference)\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTRAP Primer \u0026amp; Control MIX (NNC109) — 300 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTRAP qPCR Reaction Replenisher (NND059) — 650 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 200 reactions | Method: qPCR (Real-time Quantitative TRAP, RQ-TRAP)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only — read Material Safety Data Sheets (MSDS) and follow laboratory safety procedures; wear PPE.\u003c\/p\u003e\r\n \u003cp\u003e• Maintain physically separated areas for sample preparation, positive area, negative area and PCR amplification to avoid cross-contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Use designated pipettes and aerosol-resistant RNase-free tips for TSR8 and standards; do not reuse those pipettes in other steps.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Cell Lysis Buffer Working Solution by adding 5 μL RNase inhibitor to 1 mL Cell Lysis Buffer and keep on ice; use on ice to preserve telomerase activity.\u003c\/p\u003e\r\n \u003cp\u003e• Heat inactivate each sample extract at 85 °C for 10 minutes to generate negative control (required).\u003c\/p\u003e\r\n \u003cp\u003e• Avoid repeated freeze–thaw of cell pellets and extracts; cell pellets may be stored at –70 °C up to one year; extracts aliquoted and stored at –70 °C up to 6 months.\u003c\/p\u003e\r\n \u003cp\u003e• Clean PCR tube racks with 10% bleach and UV irradiation after each use; prevent TSR8 and PCR product contamination of pre-PCR areas.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific settings and analysis parameters; default threshold recommended is 0.1 and use Manual Ct with Automatic Baseline for analysis (as example).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1802950.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1802950.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the required sample input and preparation?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStart from freshly harvested 10^6 cells with viability ≥80% for extraction. Rinse cells with PBS, lyse with Cell Lysis Buffer Working Solution, incubate on ice 30 minutes, centrifuge and collect supernatant as extract. For high-activity samples consider 10-fold dilution or extracting from 10^5 cells.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay dynamic range and detection limit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe standard series covers 0.4 to 400,000 TPG Units (ST6–ST0). The lowest standard provided corresponds to 0.4 TPG Units (0.0002 amol\/μL TSR8). Confirm instrument-specific LOD\/LOQ during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reagents be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at –20 °C (protect from light where indicated). Cell pellets can be stored at –70 °C up to one year; prepared extracts should be aliquoted and kept at –70 °C (no more than 6 months) to avoid freeze–thaw.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls are required in each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Heat-treated negative quality control (HT sample), Positive quality control (telomerase positive cell extract) and a TSR8 standard curve (at least five concentrations). Internal control (CY5) monitors inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"200 Reactions","offer_id":45186824011957,"sku":"1802950","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/telomerase-assay-kit.jpg?v=1742264631"},{"product_id":"reverse-transcriptase-assay-kit-shentek®","title":"Reverse Transcriptase Assay kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px 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display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 12 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Reverse Transcriptase Assay Kit is a research-use kit for detecting reverse transcriptase (RT) activity using MS2 RNA as template, followed by reverse transcription and fluorescent qPCR detection. The assay follows the reverse transcriptase activity test method referenced in the Chinese Pharmacopoeia. The kit is designed for testing animal cell matrices used during production and verification of biological products. Note: certain cell types (e.g., chicken embryonic fibroblasts, other avian-origin cells, and some rodent-derived cell lines) may show endogenous RT activity and can test positive; users should follow applicable regulatory requirements when interpreting results.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eDetection of reverse transcriptase activity in cell matrices and related samples using MS2 RNA template, reverse transcription, and fluorescent qPCR\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eValidated sensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated that all 10 replicate RT samples of ST8 (10^4 pU\/μL) are detected (10\/10)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (typical)\u003c\/td\u003e\r\n \u003ctd\u003eTypical recommended standard points ST3–ST8 corresponding to a 10-fold serial dilution series ending at ST8 = 10^4 pU\/μL (implied range approximately 10^9 to 10^4 pU\/μL depending on ST0 preparation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard curve R² ≥ 0.96; Positive control sample (PCS) Ct ≤ 28; sensitivity sample Ct ≤ 38\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eResult interpretation thresholds\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNegative: no Ct or Ct ≥ 40 without amplification curve. Positive: Ct \u0026lt; 40 with clear amplification curve. Borderline: Ct between 38–40 -\u0026gt; repeat test; if repeated Ct \u0026lt; 40 with clear curve then positive.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRT incubation\u003c\/td\u003e\r\n \u003ctd\u003e37 °C for 4 hours (to produce reverse transcription product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total volume per qPCR well (25 μL qPCR mix + 5 μL RT product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRT reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e25 μL total per RT reaction (20 μL RT-MIX + 5 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling program (example, ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eRNase digestion 37 °C 07:00 (1 cycle); Activation 95 °C 05:00 (1 cycle); 50 cycles: Denature 95 °C 00:20, Anneal\/Read 57 °C 01:00 (fluorescence read), Extend 72 °C 00:10; Final extend 72 °C 02:00 (1 cycle)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reagents composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003e2×qPCR SHENmix 15 μL; MS2 Primer \u0026amp; Probe MIX 3 μL; RNase A (10 mg\/mL, user-supplied) 1 μL; 100×ROX 0–0.3 μL (instrument dependent); ddH2O to 25 μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRNase handling\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR Reaction MIX contains RNase A (user-supplied addition). Prepare\/spike RNase A separate from reverse transcription workspace to avoid contamination.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMS2 RNA template-based assay:\u003c\/strong\u003e Uses MS2 RNA as an external template for sensitive detection of reverse transcriptase activity.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReverse transcription followed by fluorescent qPCR:\u003c\/strong\u003e Assay couples reverse transcription with fluorescent qPCR for quantitative detection and standard curve absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplies with referenced pharmacopoeia method:\u003c\/strong\u003e Protocol is aligned with the reverse transcriptase activity test method described in the Chinese Pharmacopoeia.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated controls and mixes:\u003c\/strong\u003e Includes M-MLVRT control, MS2 Primer \u0026amp; Probe mix, and 2×qPCR SHENmix to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated sensitivity procedure:\u003c\/strong\u003e Includes recommended sensitivity validation (10 replicates of lowest standard ST8) to confirm assay performance on first use.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for cell matrix testing:\u003c\/strong\u003e Intended for screening reverse transcriptase activity in animal cell matrices used in biological product development and verification.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eDetection of reverse transcriptase activity in cell culture supernatants or cell matrix preparations\u003c\/li\u003e\r\n \u003cli\u003eScreening of cell substrates for retroviral reverse transcriptase activity during biological product production and release testing\u003c\/li\u003e\r\n \u003cli\u003eVerification testing of biological raw materials and process intermediates for RT contamination\u003c\/li\u003e\r\n \u003cli\u003eResearch applications investigating retroviral-like activity in samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eM-MLVRT Control (NNA024), 6 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (NND010), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBuffer A (NND012), 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBuffer B (NND013), 750 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMS2 RNA (NND011), 15 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReverse Transcription Buffer (NNB010), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMS2 Primer \u0026amp; Probe MIX (NNC036), 150 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C (or per label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e2×qPCR SHENmix (NNC045), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e100×ROX (NND007), 20 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: Reverse transcription followed by fluorescent qPCR (absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use RNase-free consumables and work in RNase-controlled environment; UV-irradiate surfaces and equipment and disinfect with 75% ethanol as recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and spike RNase A into qPCR mix in an area separate from reverse transcription operations to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (e.g., ROX, reverse transcription buffer) from light and store as indicated.\u003c\/p\u003e\r\n \u003cp\u003e• Positive controls should be aliquoted and stored at −65 °C; contact manufacturer to order PC if needed.\u003c\/p\u003e\r\n \u003cp\u003e• Certain cell types (e.g., chicken embryonic fibroblasts, avian-origin cells, and some rodent cell lines) may contain endogenous retroviral sequences and commonly test positive; interpret according to regulatory guidance.\u003c\/p\u003e\r\n \u003cp\u003e• If test sample Ct is between 38–40, repeat the test. Confirm positive only if repeat Ct \u0026lt; 40 with clear amplification curve.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1505700.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1505700.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the kit and its components?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore frozen components (e.g., M-MLVRT Control, MS2 RNA, Reverse Transcription Buffer) at −20 °C. Other reagents such as primers\/probe mix and 2×qPCR SHENmix should be stored per label (typically 2–8 °C). Protect light-sensitive reagents (e.g., ROX, RT buffer) from light. The kit is stable for up to 12 months under appropriate storage.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the validated detection sensitivity of the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit validation procedure requires that all 10 replicate RT samples of ST8 (10^4 pU\/μL) are detected (10\/10). ST8 is used as the sensitivity check; users should perform the sensitivity test on first use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted (positive\/negative)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNegative: no Ct or Ct ≥ 40 without an amplification curve. Positive: Ct \u0026lt; 40 with a clear amplification curve. If Ct is between 38–40, repeat the test; if the repeat Ct \u0026lt; 40 with a clear curve, consider the sample positive.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria must be met for a valid run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandard curve R² ≥ 0.96; Positive control (PCS) Ct ≤ 28; sensitivity sample Ct ≤ 38. If these criteria are not met, the run should be considered invalid and repeated.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Method aligned with reverse transcriptase activity test method referenced in the Chinese Pharmacopoeia.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45186868412597,"sku":"1505700","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Reverse_Transcriptase_Assay_kit.jpg?v=1742265588"},{"product_id":"reverse-transcriptase-assay-control-shentek®","title":"Reverse Transcriptase Assay Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 18 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eReverse Transcriptase Positive Control (PC) is provided to ensure the accuracy and reliability of reverse transcriptase activity assays. The control is prepared in accordance with the reverse transcriptase activity test method in the Chinese Pharmacopoeia. SP2\/0 cell culture supernatant is collected, supplemented with protective agent and frozen at -65 °C or below. The control is intended to be diluted and used alongside test samples and standard curve in reverse transcription\/qPCR workflows. For detailed assay procedures refer to the Reverse Transcriptase Assay Kit User Guide (Product No. 1505700). For Research Use Only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd\u003e1505701; Part No. NNA025\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFormulation \/ Source\u003c\/td\u003e\r\n \u003ctd\u003eSP2\/0 cell culture supernatant with protective agent\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eVolume per vial\u003c\/td\u003e\r\n \u003ctd\u003e25 μL × 1 tube\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage temperature\u003c\/td\u003e\r\n \u003ctd\u003e-65 °C or below\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ Stability\u003c\/td\u003e\r\n \u003ctd\u003eUp to 18 months when stored at -65 °C or below\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control acceptance criterion\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClear amplification curve and qPCR Ct ≤ 28\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended working dilution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 5 μL PC to 45 μL Buffer A (1:10 dilution) prior to use\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003ePositive control for reverse transcriptase activity assays and RT-qPCR workflows\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePharmacopoeia‑aligned preparation:\u003c\/strong\u003e Prepared in accordance with the reverse transcriptase activity test method in the Chinese Pharmacopoeia to provide a standardized control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCell culture supernatant matrix:\u003c\/strong\u003e Derived from SP2\/0 cell culture supernatant and supplemented with protective agent to mimic assay matrix.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-dilute format:\u003c\/strong\u003e Supplied as a concentrated positive control (25 μL) that is diluted 1:10 prior to assay, facilitating parallel processing with samples and standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Clear amplification curve with Ct ≤ 28 required to qualify the control run.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong-term frozen stability:\u003c\/strong\u003e Stable when stored at -65 °C or below for up to 18 months.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003ePositive control for reverse transcriptase activity assays\u003c\/li\u003e\r\n \u003cli\u003eQuality control in RT-qPCR workflows\u003c\/li\u003e\r\n \u003cli\u003eAssay validation and performance monitoring\u003c\/li\u003e\r\n \u003cli\u003eRoutine laboratory QC for reverse transcription and amplification steps\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReverse Transcriptase Positive Control (NNA025)\u003c\/span\u003e\u003cstrong\u003e-65 °C (25 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not specified | Method: Reverse transcriptase activity assay (used with RT-qPCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\r\n \u003cp\u003e• Read the Material Safety Data Sheet (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate personal protective equipment (gloves, eyewear, lab coat, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Store product at -65 °C or below. Do not refreeze repeatedly; aliquot into single‑use volumes on first thaw in nuclease‑free, protease‑free cryotubes.\u003c\/p\u003e\r\n \u003cp\u003e• Use nuclease-free reagents and consumables. Prepare working dilution by adding 5 μL PC to 45 μL Buffer A and mix thoroughly (pipette mixing, vortex, brief spin).\u003c\/p\u003e\r\n \u003cp\u003e• Perform reverse transcription reactions simultaneously with test samples and the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• QC acceptance: run is qualified if PC produces a clear amplification curve and Ct ≤ 28. If Ct \u0026gt; 28, verify aliquoting and storage conditions; repeat the test to exclude operator error.\u003c\/p\u003e\r\n \u003cp\u003e• Kit components are stable for up to 18 months when stored as recommended.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1505701.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1505701.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the Reverse Transcriptase Positive Control?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore at -65 °C or below. On first use, aliquot into single-use nuclease-free, protease-free cryotubes to avoid repeated freeze-thaw cycles.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare the working control for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the control by adding 5 μL of PC to 45 μL of Buffer A (1:10). Mix by pipetting 10 times, vortex briefly and quick spin as described in the user guide.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should the control meet?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe positive control should produce a clear amplification curve and a qPCR Ct value ≤ 28. If Ct \u0026gt; 28, check storage\/handling and repeat the test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is this product suitable for clinical diagnostics?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for Research Use Only (RUO) and is not for diagnostic or clinical use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); prepared in accordance with the reverse transcriptase activity test method in the Chinese Pharmacopoeia; Not for diagnostic or clinical use.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"25μL × 1 tube","offer_id":45186896691381,"sku":"1505701","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Telomerase_Assay_kit.jpg?v=1742266013"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-manual-operation-shentek®","title":"Residual Host Cell DNA Sample Preparation Kit (For manual operation) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic-particle based sample preparation kit designed for efficient and reproducible recovery of trace residual host-cell DNA from a wide range of biological products and complex sample matrices across multiple manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended to be used with SHENTEK® host cell DNA quantitation and size analysis kits. The kit supports multiple host cell types (CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA, etc.), and includes reagents for Proteinase K digestion, DNA binding, washing and elution, with magnetic particles for nucleic acid capture.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input (typical)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (procedure described: add 100 µL sample + 10 µL 5M NaCl before digestion)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepare Proteinase K digestion solution in Proteinase K Buffer. For samples with 0–100 mg\/mL protein add 10 µL Proteinase K per 100 µL buffer; for 100–200 mg\/mL protein add 20 µL Proteinase K per 100 µL buffer. Incubate digestion at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA. Note: Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles usage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAdd 10 µL magnetic particles per sample. Vortex particles 5 seconds to fully resuspend before use. Store magnetic particles at 2–8 °C.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAlcohol addition for binding\u003c\/td\u003e\r\n \u003ctd\u003eAfter binding buffer, add 200 µL isopropanol per sample to facilitate binding.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding incubation\u003c\/td\u003e\r\n \u003ctd\u003eVortex tubes vertically at medium speed for 5 minutes to bind nucleic acids to magnetic particles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash once with 700 µL Wash buffer A (ethanol to be added as instructed), then wash with 700 µL Wash buffer B (70% ethanol prepared separately). Spin 10 seconds between steps and use magnetic stand to separate beads.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBead drying\u003c\/td\u003e\r\n \u003ctd\u003eAir-dry magnetic pellet at room temperature for 30 seconds to 3 minutes (environment dependent) to remove residual ethanol; avoid over-drying which hinders resuspension.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 50–100 µL pre-warmed (70 °C) Elution buffer, vortex 5 seconds, incubate at 70 °C for 7 minutes with intermittent vortexing. Centrifuge 1 min, separate beads on magnetic stand and transfer eluate.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical centrifugation\u003c\/td\u003e\r\n \u003ctd\u003eQuick spins of 10 seconds are used throughout to collect liquids from caps\/walls; final centrifuge after elution 1 minute.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReagent storage conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eWash Buffer A: room temperature (add 40 mL anhydrous ethanol before use); Binding solution \/ Elution buffer \/ Dilution buffer \/ Proteinase K Buffer: room temperature (as labeled); Magnetic particles: 2–8 °C; Proteinase K: (no explicit temperature on label in table) - recommended follow label; Glycogen: -20 °C; Yeast tRNA: per label.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under their appropriate conditions for up to 24 months (check label expiration dates).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH requirement\u003c\/td\u003e\r\n \u003ctd\u003eAdjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction if sample pH \u0026lt;5 or \u0026gt;9.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n \u003ctd\u003eUse Negative Control Sample (NCS) processed in parallel and Sample Extraction Recovery Control (ERC) spiked at 2–10× the amount quantified in the unspiked sample to monitor extraction recovery and accuracy.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic-particle based extraction:\u003c\/strong\u003e Magnetic particle separation technology enables efficient capture and reproducible recovery of trace residual DNA from complex biological matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad host-cell compatibility:\u003c\/strong\u003e Validated for multiple host cells including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Compatible with manual preparation or automated extraction using the rHCDpurify instrument.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized for downstream quantitation:\u003c\/strong\u003e Extracted DNA is suitable for SHENTEK® host cell DNA quantitation and size analysis kits and analysis on real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-configured reagents for 100 extractions:\u003c\/strong\u003e Complete reagent set supplied for 100 DNA extractions, with specific buffers and additives (Glycogen, tRNA) for improved recovery of low-abundance DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eResidual host cell DNA extraction and purification from biologics and process intermediates\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for host-cell DNA quantitation by qPCR\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring for biologics and vaccine manufacturing\u003c\/li\u003e\r\n \u003cli\u003eSize analysis and downstream molecular assays of residual DNA\u003c\/li\u003e\r\n \u003cli\u003eExtraction from diverse matrices including upstream and downstream process samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash Buffer A (NND014) 30 mL × 1 bottle (add 40 mL anhydrous ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding Solution (NND016) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution Buffer (NND018) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution Buffer (NND021) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND031) 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label); prepare fresh digestion solution as instructed\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle separation (sample extraction)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, clothing, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash Buffer A before first use; prepare 70% Wash Buffer B separately and label.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and use 100% isopropanol for binding step as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure reagents are clear before use; if cloudy, heat at 37 °C until clear and mix well.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) if outside range to avoid affecting extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid over-drying magnetic pellet; air-dry 30 seconds to 3 minutes depending on environment.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays on the same day after nucleic acid extraction for accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storage below -18 °C may degrade performance.\u003c\/p\u003e\r\n \u003cp\u003e• Use low-retention filter tips and regularly calibrate pipettes to ensure accurate spiking and aspiration.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is recommended per extraction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes using 100 µL of sample per extraction. Adjustments may be made with proportional reagent preparation; follow kit guidelines for dilution and recovery controls.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I use this kit for E. coli and yeast DNA extraction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit supports E. coli and yeast DNA extraction; note that Yeast tRNA should not be added to the Binding buffer when extracting E. coli or yeast DNA.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended incubation conditions for Proteinase K digestion?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution according to sample protein concentration and incubate at 55 °C for 60 minutes as described in the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store the magnetic particles and other reagents?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Glycogen is stored at -20 °C. Other liquid buffers are stored at room temperature as labeled. The kit components can be stored under appropriate conditions for up to 24 months; always check expiration dates on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186936340661,"sku":"SK030203D100","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ResidualHostCellDNASamplePreparationKit_Formanualoperation_-SHENTEK.jpg?v=1742276761"}],"url":"https:\/\/biofargo.com\/collections\/residual-host-cell-dna-quantitation.oembed?page=3","provider":"Biofargo","version":"1.0","type":"link"}