{"title":"qRT-PCR Kit","description":"\u003ch2\u003eLooking for a Custom qPCR or RT-PCR Kit?\u003c\/h2\u003e\n\u003cp\u003eWhether you're detecting rare organisms, using special sample types, or running multiplex reactions, Biofargo offers custom qPCR kit development tailored to your research or diagnostic needs.\u003c\/p\u003e\n\u003ch3\u003eCustomizable Options:\u003c\/h3\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜\u003c\/span\u003e \u003c\/strong\u003e\u003c\/span\u003ePathogen target(s): any virus, bacterium, fungus, or parasite\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜ \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003eDetection chemistry: SYBR Green or TaqMan probe\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜ \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003eFormat: singleplex or multiplex\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜ \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003eSample types: blood, swabs, food, water, plant, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜ \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003eMachine compatibility: ABI, Bio-Rad, Roche, and more\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e➜ \u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003ePackaging: liquid or lyophilized, custom volumes or labeling\u003c\/p\u003e\n\u003ch3\u003eRequest Your Custom Kit\u003c\/h3\u003e\n\u003cp\u003eFill out the short form below and our team will get back to you within 24–48 hours.\u003c\/p\u003e\n\u003cp\u003eEmail us directly at: \u003cstrong\u003econtact@biofargo.com\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eNot sure what you need? Just describe your goals — we’ll help you design the right solution.\u003c\/p\u003e\n\u003ch3\u003eStill Searching?\u003c\/h3\u003e\n\u003cp\u003eOur database includes over 4,700 validated targets. If you can’t find a specific product, use the site search or contact us for assistance.\u003c\/p\u003e","products":[{"product_id":"skygen-chlamydia-spp-universal-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia spp.Universal Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11300 and is now updated to BF-34377554 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11300.pdf\"\u003e\u003cstrong\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11300.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097646261,"sku":"BF-34377554","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d57509db-6b33-4ab9-8fa0-48ac7597d6d9.jpg?v=1774176078"},{"product_id":"skygen-chlamydia-muridarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia muridarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia muridarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11340 and is now updated to BF-59544059 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11340.pdf\"\u003e\u003cstrong\u003eChlamydia muridarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11340.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097711797,"sku":"BF-59544059","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_856da674-f3d3-403b-a937-de0fbb07fe22.jpg?v=1774204110"},{"product_id":"skygen-chlamydia-caviae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia caviae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia caviae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11350 and is now updated to BF-43373408 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11350.pdf\"\u003e\u003cstrong\u003eChlamydia caviae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11350.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097810101,"sku":"BF-43373408","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_801f5bde-c74e-4130-bb25-0904a40b2d6e.jpg?v=1774157739"},{"product_id":"skygen-aspergillus-campestris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Aspergillus campestris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eAspergillus campestris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26780 and is now updated to BF-47481291 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26780.pdf\"\u003e\u003cstrong\u003eAspergillus campestris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26780.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151287477,"sku":"BF-47481291","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0226ce1c-921c-4fc0-829f-8d02d108d827.jpg?v=1774190208"},{"product_id":"skygen-cryptosporidium-galli-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium galli Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium galli Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26811 and is now updated to BF-66087926 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26811.pdf\"\u003e\u003cstrong\u003eCryptosporidium galli Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26811.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151910069,"sku":"BF-66087926","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0dacbbc6-fad2-40fd-90cd-32262c9bb1a9.jpg?v=1774186735"},{"product_id":"skygen-cryptosporidium-hominis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium hominis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26812 and is now updated to BF-76159985 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26812.pdf\"\u003e\u003cstrong\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26812.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152008373,"sku":"BF-76159985","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_57c29d05-f6fd-4774-9ab4-93002125db01.jpg?v=1774191062"},{"product_id":"skygen-cryptosporidium-molnari-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium molnari Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26814 and is now updated to BF-26550785 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26814.pdf\"\u003e\u003cstrong\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26814.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152041141,"sku":"BF-26550785","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b81128eb-9cce-4ccd-9f38-df246ccb93fa.jpg?v=1774160359"},{"product_id":"skygen-cryptosporidium-meleagridis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium meleagridis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26815 and is now updated to BF-60951899 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26815.pdf\"\u003e\u003cstrong\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26815.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152073909,"sku":"BF-60951899","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4d6e1dfc-516b-4784-bab8-0a2514d20233.jpg?v=1774177384"},{"product_id":"skygen-cryptosporidium-muris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium muris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium muris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26816 and is now updated to BF-33047002 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26816.pdf\"\u003e\u003cstrong\u003eCryptosporidium muris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26816.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152106677,"sku":"BF-33047002","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bfbcfe8a-2f8d-48ee-ae51-212d38b61faf.jpg?v=1774202367"},{"product_id":"skygen-cryptosporidium-nasorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium nasorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26817 and is now updated to BF-79876578 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26817.pdf\"\u003e\u003cstrong\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26817.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152139445,"sku":"BF-79876578","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_c17b4c37-67b8-4a1e-b8af-8877a07ce02c.jpg?v=1774192338"},{"product_id":"skygen-cryptosporidium-occultus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium occultus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26818 and is now updated to BF-83455011 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26818.pdf\"\u003e\u003cstrong\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26818.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152172213,"sku":"BF-83455011","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4e7d04d4-9142-4465-8f00-690b68a2fe2f.jpg?v=1774201908"},{"product_id":"skygen-cryptosporidium-ryanae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ryanae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26819 and is now updated to BF-61866087 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26819.pdf\"\u003e\u003cstrong\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26819.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152204981,"sku":"BF-61866087","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8d59fbe0-0b16-4251-ba27-c36dda80c31e.jpg?v=1774206225"},{"product_id":"skygen-cryptosporidium-suis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium suis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium suis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26821 and is now updated to BF-19266672 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26821.pdf\"\u003e\u003cstrong\u003eCryptosporidium suis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26821.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152303285,"sku":"BF-19266672","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d7482826-c68f-4b4e-87e2-a698eb209f6f.jpg?v=1774201916"},{"product_id":"skygen-cryptosporidium-scophthalmi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scophthalmi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26822 and is now updated to BF-94766909 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26822.pdf\"\u003e\u003cstrong\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26822.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152336053,"sku":"BF-94766909","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_61cc6c72-cb76-4a84-8f36-5b1f7a1103db.jpg?v=1774177268"},{"product_id":"skygen-cryptosporidium-saurophilum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium saurophilum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26823 and is now updated to BF-46075436 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26823.pdf\"\u003e\u003cstrong\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26823.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152368821,"sku":"BF-46075436","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e47a134c-3ffb-48db-838c-75102c21767c.jpg?v=1774206816"},{"product_id":"skygen-cryptosporidium-scrofarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scrofarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26824 and is now updated to BF-91078992 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26824.pdf\"\u003e\u003cstrong\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26824.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152401589,"sku":"BF-91078992","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ccebb0e2-16d8-4627-96c1-ad2d2c35701d.jpg?v=1774200598"},{"product_id":"skygen-cryptosporidium-ubiquitum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ubiquitum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26825 and is now updated to BF-22799761 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26825.pdf\"\u003e\u003cstrong\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26825.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152434357,"sku":"BF-22799761","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_59b61532-0e3b-4f22-b1cc-33fe450bbd97.jpg?v=1774191859"},{"product_id":"skygen-cryptosporidium-varaii-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium varaii Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26826 and is now updated to BF-20436497 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26826.pdf\"\u003e\u003cstrong\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26826.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152467125,"sku":"BF-20436497","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_90a4f4a7-c3e5-4bdb-8a21-742ef231a111.jpg?v=1774191497"},{"product_id":"skygen-cryptosporidium-viatorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium viatorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26827 and is now updated to BF-51393006 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26827.pdf\"\u003e\u003cstrong\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26827.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152696501,"sku":"BF-51393006","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_00593ebd-f248-45a2-9826-dd3e74f20d53.jpg?v=1774205906"},{"product_id":"skygen-cryptosporidium-wrairi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium wrairi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26828 and is now updated to BF-42857399 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26828.pdf\"\u003e\u003cstrong\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26828.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152794805,"sku":"BF-42857399","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_aa5a97d3-4f64-4c17-8fc4-bab8947c969b.jpg?v=1774171625"},{"product_id":"skygen-cryptosporidium-andersoni-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium andersoni Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26850 and is now updated to BF-56975258 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26850.pdf\"\u003e\u003cstrong\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26850.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153024181,"sku":"BF-56975258","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6cc007a5-0583-4244-997d-9fc1d510e6e2.jpg?v=1774204766"},{"product_id":"skygen-cryptosporidium-bovis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium bovis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26860 and is now updated to BF-59618136 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26860.pdf\"\u003e\u003cstrong\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26860.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153089717,"sku":"BF-59618136","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_769c899e-1b3d-45c4-af3e-7be8b290204e.jpg?v=1774189072"},{"product_id":"skygen-cryptosporidium-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26870 and is now updated to BF-53887329 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26870.pdf\"\u003e\u003cstrong\u003eCryptosporidium canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26870.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153188021,"sku":"BF-53887329","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_50324e85-c3cf-44c6-85d4-4aa1c0906d50.jpg?v=1774189063"},{"product_id":"skygen-cryptosporidium-felis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium felis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium felis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26880 and is now updated to BF-39439124 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26880.pdf\"\u003e\u003cstrong\u003eCryptosporidium felis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26880.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153220789,"sku":"BF-39439124","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_da2b8561-2924-43d9-8c04-d20f6aa0ac11.jpg?v=1774192285"},{"product_id":"skygen-cryptosporidium-fayeri-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium fayeri Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26890 and is now updated to BF-60900951 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26890.pdf\"\u003e\u003cstrong\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26890.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153253557,"sku":"BF-60900951","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_930ae5ab-4847-4abc-8cd5-ae507e7a812b.jpg?v=1774193639"},{"product_id":"skygen-hepatozoon-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Hepatozoon canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHepatozoon canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58000 and is now updated to BF-87699077 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58000.pdf\"\u003e\u003cstrong\u003eHepatozoon canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282355445941,"sku":"BF-87699077","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_96b39454-3724-4d36-82f1-fa76dc84ab28.jpg?v=1774204757"},{"product_id":"skygen-pichia-pastoris-probe-rt-pcr-kit-without-internal-control","title":"Pichia pastoris Probe RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003ePichia pastoris Probe RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58900 and is now updated to BF-75676982 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58900.pdf\"\u003e\u003cstrong\u003ePichia pastoris Probe RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58900.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357051573,"sku":"BF-75676982","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b17d638d-769d-44aa-bbb8-b1e2239b1410.jpg?v=1774187579"},{"product_id":"skygen-saccharomyces-cerevisiae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Saccharomyces cerevisiae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSaccharomyces cerevisiae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58910 and is now updated to BF-72442609 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58910.pdf\"\u003e\u003cstrong\u003eSaccharomyces cerevisiae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58910.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357739701,"sku":"BF-72442609","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b1bacb46-efd0-4dcf-9656-374d20887039.jpg?v=1774205451"},{"product_id":"skygen-schizosaccharomyces-pombe-probe-realtime-rt-pcr-kit-without-internal-control","title":"Schizosaccharomyces pombe Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSchizosaccharomyces pombe Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58920 and is now updated to BF-78477582 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58920.pdf\"\u003e\u003cstrong\u003eSchizosaccharomyces pombe Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58920.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357969077,"sku":"BF-78477582","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b8ed6fd4-f912-4280-9561-0d7065b8de48.jpg?v=1774188414"},{"product_id":"skygen-brucella-inopinata-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella inopinata Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella inopinata Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61211 and is now updated to BF-71626667 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61211.pdf\"\u003e\u003cstrong\u003eBrucella inopinata Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61211.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370388149,"sku":"BF-71626667","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6c77bc7d-7830-4bb1-9c8d-fd73e65464d8.jpg?v=1774164498"},{"product_id":"skygen-brucella-vulpis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella vulpis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella vulpis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61212 and is now updated to BF-11064734 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61212.pdf\"\u003e\u003cstrong\u003eBrucella vulpis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61212.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370420917,"sku":"BF-11064734","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_20c53d37-2c7f-4234-b07a-831bb1a5bf11.jpg?v=1774187674"},{"product_id":"skygen-brucella-papionis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella papionis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella papionis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61213 and is now updated to BF-21408354 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61213.pdf\"\u003e\u003cstrong\u003eBrucella papionis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61213.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370519221,"sku":"BF-21408354","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_521fcf88-9fdb-42f4-b61d-bb3a9a6c20a3.jpg?v=1774187639"},{"product_id":"skygen-brucella-melitensis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella melitensis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella melitensis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61260 and is now updated to BF-20115717 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61260.pdf\"\u003e\u003cstrong\u003eBrucella melitensis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61260.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370912437,"sku":"BF-20115717","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_66ac9a80-e377-42e4-ac15-5b66fe056fa8.jpg?v=1774176980"},{"product_id":"skygen-brucella-maris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella maris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella maris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61270 and is now updated to BF-28670061 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61270.pdf\"\u003e\u003cstrong\u003eBrucella maris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61270.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371109045,"sku":"BF-28670061","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5ce8460e-55f1-4ed1-922b-f4f1d82d7819.jpg?v=1774163972"},{"product_id":"skygen-brucella-ceti-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella ceti Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella ceti Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61280 and is now updated to BF-52942801 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61280.pdf\"\u003e\u003cstrong\u003eBrucella ceti Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61280.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371207349,"sku":"BF-52942801","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_593f4d34-6180-48b9-a558-00daaad0cf12.jpg?v=1774194504"},{"product_id":"skygen-brucella-pinnipedialis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella pinnipedialis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella pinnipedialis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61290 and is now updated to BF-71183683 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61290.pdf\"\u003e\u003cstrong\u003eBrucella pinnipedialis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61290.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371272885,"sku":"BF-71183683","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_9e92dc55-4ce7-48bd-836a-3cc43c8e940c.jpg?v=1774193016"},{"product_id":"skygen-spirillum-minus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Spirillum minus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSpirillum minus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-62005 and is now updated to BF-14937533 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-62005.pdf\"\u003e\u003cstrong\u003eSpirillum minus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-62005.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282373632181,"sku":"BF-14937533","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_f48abe93-b00b-47ed-8219-0e4813e2e876.jpg?v=1774202142"},{"product_id":"skygen-trypanosoma-lewisi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Trypanosoma lewisi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eTrypanosoma lewisi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-66011 and is now updated to BF-23536660 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-66011.pdf\"\u003e\u003cstrong\u003eTrypanosoma lewisi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-66011.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282382545077,"sku":"BF-23536660","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4c136fa2-f9aa-4d2f-9e49-779db452005d.jpg?v=1774166591"},{"product_id":"skygen-nontuberculous-mycobacteria-probe-realtime-rt-pcr-kit-without-internal-control","title":"Nontuberculous mycobacteria Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eNontuberculous mycobacteria Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-68805 and is now updated to BF-74731677 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-68805.pdf\"\u003e\u003cstrong\u003eNontuberculous mycobacteria Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-68805.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282393817269,"sku":"BF-74731677","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_1a2a902e-54c7-49df-a8a9-4f17aafe6302.jpg?v=1774193497"},{"product_id":"skygen-toxocara-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Toxocara canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eToxocara canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-75900 and is now updated to BF-23579083 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-75900.pdf\"\u003e\u003cstrong\u003eToxocara canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-75900.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282409218229,"sku":"BF-23579083","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b248119d-14bd-45f7-811c-3d2553ac1ca6.jpg?v=1774196480"},{"product_id":"skygen-human-coronavirus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Human Coronavirus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHuman Coronavirus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-88100 and is now updated to BF-30480550 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-88100.pdf\"\u003e\u003cstrong\u003eHuman Coronavirus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-88100.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282423406773,"sku":"BF-30480550","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_f97eaeed-ee95-4514-a6f5-119ee8078e73.jpg?v=1774154510"},{"product_id":"sars-cov-2-s-n-e-gene-triplex-probe-rt-pcr-kit","title":"SARS-CoV-2 S\/N\/E Gene Triplex Probe RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSARS-CoV-2 S\/N\/E Gene Triplex Probe RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-81902 and is now updated to BF-95506507 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-81902.pdf\"\u003e\u003cstrong\u003eSARS-CoV-2 S\/N\/E Gene Triplex Probe RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-81902.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51243965874357,"sku":"BF-95506507","price":955.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8b6839b1-0460-41ec-98fe-e806a222f6bd.jpg?v=1774198763"},{"product_id":"nervous-necrosis-virusnnv-probe-realtime-rt-pcr-kit-without-internal-control","title":"Nervous Necrosis Virus(NNV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eNervous Necrosis Virus(NNV) Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-55240 and is now updated to BF-28723324 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-55240.pdf\"\u003e\u003cstrong\u003eNervous Necrosis Virus(NNV) Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-55240.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51243969052853,"sku":"BF-28723324","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ef9ce5da-e215-4d21-8f1e-16eac3626caf.jpg?v=1774177937"},{"product_id":"enterovirus-91-ev91probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 91 (EV91)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 91 (EV91)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15595 and is now updated to BF-18570944 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15595.pdf\"\u003e\u003cstrong\u003eEnterovirus 91 (EV91)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15595.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244025741493,"sku":"BF-18570944","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_28ca27b7-5e47-44fc-95f5-fce15f6a3215.jpg?v=1774168282"},{"product_id":"enterovirus-90-ev90probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 90 (EV90)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 90 (EV90)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15594 and is now updated to BF-83974999 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15594.pdf\"\u003e\u003cstrong\u003eEnterovirus 90 (EV90)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15594.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244026003637,"sku":"BF-83974999","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_c8bd6672-cf0f-458b-920d-c58d15538d54.jpg?v=1774156015"},{"product_id":"enterovirus-89-ev89probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 89 (EV89)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 89 (EV89)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15593 and is now updated to BF-34503885 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15593.pdf\"\u003e\u003cstrong\u003eEnterovirus 89 (EV89)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15593.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244026691765,"sku":"BF-34503885","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d91e0436-3abc-443f-8a40-65067e06c767.jpg?v=1774175044"},{"product_id":"enterovirus-88-ev88probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 88 (EV88)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 88 (EV88)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15592 and is now updated to BF-49091815 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15592.pdf\"\u003e\u003cstrong\u003eEnterovirus 88 (EV88)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15592.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244026822837,"sku":"BF-49091815","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_a52bf2c1-3fec-4f82-a93e-4dd841a88fcb.jpg?v=1774161556"},{"product_id":"enterovirus-87-ev87probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 87 (EV87)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 87 (EV87)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15591 and is now updated to BF-63883915 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15591.pdf\"\u003e\u003cstrong\u003eEnterovirus 87 (EV87)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15591.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244026855605,"sku":"BF-63883915","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_168b73f1-2007-46f4-b96c-7bb960755f19.jpg?v=1774199127"},{"product_id":"enterovirus-86-ev86probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 86 (EV86)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 86 (EV86)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15590 and is now updated to BF-29614848 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15590.pdf\"\u003e\u003cstrong\u003eEnterovirus 86 (EV86)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15590.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244026888373,"sku":"BF-29614848","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5b7bb06e-d82a-4032-b344-1989cdbbd566.jpg?v=1774175017"},{"product_id":"enterovirus-85-ev85probe-realtime-rt-pcr-kit-without-internal-control","title":"Enterovirus 85 (EV85)Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEnterovirus 85 (EV85)Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-15589 and is now updated to BF-48261801 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized  target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-15589.pdf\"\u003e\u003cstrong\u003eEnterovirus 85 (EV85)Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-15589.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244027871413,"sku":"BF-48261801","price":661.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_508bbc28-42c2-4329-afff-35da5e6de061.jpg?v=1774206666"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/IMG_2500_04c89d9f-fdc4-48fd-a641-168f81bd6af8.png?v=1757010061","url":"https:\/\/biofargo.com\/collections\/qrt-pcr-kit.oembed?page=22","provider":"Biofargo","version":"1.0","type":"link"}