{"title":"qPCR Quantitation \u0026 Analysis Kits","description":"\u003cp\u003eDiscover high-performance qPCR kits for rapid detection of bacteria, fungi, adventitious viruses, and replication-competent viruses. Our kits also include advanced assays for residual host cell protein quantitation and process-related residue analysis to ensure precise and reliable results.\u003c\/p\u003e","products":[{"product_id":"qbtesttm-x-green-ii-transparent-tubes-100t-uelandy-q2038s","title":"Quantitative Fluorescence Reagent-100T-Q2038S","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II dsDNA Quantitation Kit Plus is sensitive fluorescence detection method which can be used to quantify the dsDNA. In molecular biology, it is commonly used in the construction of cDNA library and the purification and application of subcloned DNA fragments, such as DNA quantification, product amplification and further detection of primers. The conventional DNA content detection method is to measure its absorbance at 260 nm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe main disadvantage of this method is that nucleotides, single-stranded nucleic acids and proteins have a great influence on the signal, and they are also interfered by for Research Use only contaminants in the nucleic acid preparation process.Unable to distinguish between DNA and RNA, and the sensitivity is low (5 μg\/mL dsDNAA260=0.1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II emits fluorescence only after binding dsDNA, and the fluorescence intensity is proportional to the DNA concentration. QbtestTM X-Green II dsDNA Quantification Kit Plus can detect dsDNA in the range of 10 pg\/μL-100 ng\/μL, and the linear relationship is good (R2\u0026gt;0.99). The kit can be used for Qubit and can replace imported similar products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePlease try to avoid light to slow down the quenching of fluorescence.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e1× QbtestTM X-Green II working solution is prepared when used to ensure the best results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C and protect from light, Long-term storage can be stored at -20ºC. Expiration date marked on the outer packing.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Q2038.pdf?v=1626785861\" target=\"_blank\"\u003eQ2038\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS_Q2038_Qbtest_X-Green_II_dsDNA_Quantitation_Kit_Plus.pdf.pdf?v=1760163949\"\u003eQ2038 MSDS\u003c\/a\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Uelandy","offers":[{"title":"A Group 250 µL","offer_id":40550227574965,"sku":"Q2038S-A","price":127.0,"currency_code":"USD","in_stock":true},{"title":"B Group 50 mL","offer_id":40550227607733,"sku":"Q2038S-B","price":127.0,"currency_code":"USD","in_stock":true},{"title":"C Group 1 mL","offer_id":40550227640501,"sku":"Q2038S-C","price":127.0,"currency_code":"USD","in_stock":true},{"title":"D Group 1 mL","offer_id":40550227673269,"sku":"Q2038S-D","price":127.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/biofargo.jpg?v=1761012618"},{"product_id":"qbtesttm-x-green-ii-white-tubes-500t-uelandy-q2038l","title":"Quantitative Fluorescence Reagent-500T-Q2038L","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II dsDNA Quantitation Kit Plus is sensitive fluorescence detection method which can be used to quantify the dsDNA. In molecular biology, it is commonly used in the construction of cDNA library and the purification and application of subcloned DNA fragments, such as DNA quantification, product amplification and further detection of primers. The conventional DNA content detection method is to measure its absorbance at 260 nm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe main disadvantage of this method is that nucleotides, single-stranded nucleic acids and proteins have a great influence on the signal, and they are also interfered by for Research Use only contaminants in the nucleic acid preparation process.Unable to distinguish between DNA and RNA, and the sensitivity is low (5 μg\/mL dsDNAA260=0.1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II emits fluorescence only after binding dsDNA, and the fluorescence intensity is proportional to the DNA concentration. QbtestTM X-Green II dsDNA Quantification Kit Plus can detect dsDNA in the range of 10 pg\/μL-100 ng\/μL, and the linear relationship is good (R2\u0026gt;0.99). The kit can be used for Qubit and can replace imported similar products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePlease try to avoid light to slow down the quenching of fluorescence.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e1× QbtestTM X-Green II working solution is prepared when used to ensure the best results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C and protect from light, Long-term storage can be stored at -20ºC. Expiration date marked on the outer packing.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Q2038.pdf?v=1626785861\" target=\"_blank\"\u003eQ2038\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS_Q2038_Qbtest_X-Green_II_dsDNA_Quantitation_Kit_Plus.pdf.pdf?v=1760163949\"\u003e\u003cstrong\u003eQ2038 MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Uelandy","offers":[{"title":"A Group 1.25 mL","offer_id":40550251331765,"sku":"Q2038L-A","price":412.0,"currency_code":"USD","in_stock":true},{"title":"B Group 250 mL","offer_id":40550251364533,"sku":"Q2038L-B","price":412.0,"currency_code":"USD","in_stock":true},{"title":"C Group 5 mL","offer_id":40550251397301,"sku":"Q2038L-C","price":412.0,"currency_code":"USD","in_stock":true},{"title":"D Group 5 mL","offer_id":40550251430069,"sku":"Q2038L-D","price":412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/biofargo.jpg?v=1761012618"},{"product_id":"residual-cho-dna-size-analysis-kit-2g-shentek®","title":"Residual CHO DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: 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{\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Size Analysis Kit (2G) is a fluorescent quantitative PCR (FAM) based kit for rapid and specific quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA fragments across multiple size ranges in biopharmaceutical samples (from in-process to final products). The assay amplifies four discrete fragment sizes (95 bp, 110 bp, 215 bp and 523 bp) to produce an accurate size distribution profile. The kit includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, CHO DNA control for standard curves and DNA dilution buffer (DDB). The assay sensitivity reaches femtogram (fg) level. Refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction procedures. Product No.: 1103170-2. Reagents sufficient for 4 × 100 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e95 bp, 110 bp, 215 bp, 523 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n                        \u003ctd\u003eTarget probes: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDetection limit reaches femtogram (fg) level\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (examples)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSuggested linear range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eApproximately 0.03 – 300 pg\/µL (based on provided standard curve points)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL total reaction volume (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 µL + CHO Primer\u0026amp;Probe MIX (per fragment) 2.8 µL + IPC MIX 1.3 µL = 20 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n                        \u003ctd\u003e10 µL (standard\/ST1–ST5, DDB for NCS, or purified sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/Read: 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct selection; Threshold = 0.02; Automatic baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (kit components support ~400 reactions distributed across four fragment assays)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMulti-size profiling:\u003c\/strong\u003e Simultaneous measurement strategy (four separate assays) for size distribution of CHO residual DNA (95, 110, 215, 523 bp) to characterize fragmentation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit at femtogram (fg) level enables quantitation of very low-level residual host cell DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated CHO Primer \u0026amp; Probe MIXes and qPCR Reaction Buffer simplify setup and reduce pipetting variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC mix included (VIC-labeled) to monitor inhibition and reaction performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600 plus).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA control and DNA dilution buffer for standard curves plus protocol for plate layouts and analysis.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eQuantitation and size-distribution analysis of residual CHO DNA in final drug substance\/product\u003c\/li\u003e\r\n                    \u003cli\u003eMethod development and validation for host cell DNA monitoring\u003c\/li\u003e\r\n                    \u003cli\u003eRoutine QC testing of downstream purification and final product release (research and development settings)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB002)\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light (850 μL × 8 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-95 (NNC087)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-110 (NNC088)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-215 (NNC089)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-523 (NNC090)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069)\u003c\/span\u003e\u003cstrong\u003e–20 °C (550 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO DNA Control (NNA001)\u003c\/span\u003e\u003cstrong\u003e–20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C recommended after thaw; (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 Reactions | Method: qPCR (Fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n                    \u003cp\u003e• Work in DNase-free environment; use DNase-free consumables and low-retention tips to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% alcohol before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; protect light-sensitive reagents from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at –20 °C and check expiration dates. Kit stable up to 24 months under recommended storage.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment length (95, 110, 215, 523 bp). Include at least five standard concentrations.\u003c\/p\u003e\r\n                    \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitates, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use NTC (no template control), NCS (negative control sample) and ERC (extraction reference control) as directed to monitor contamination and extraction recovery.\u003c\/p\u003e\r\n                    \u003cp\u003e• Mix qPCR reactions thoroughly, seal plate, spin briefly and avoid bubble formation prior to run.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit sensitivity reaches the femtogram (fg) level. Standard curve points provided include concentrations down to 0.03 pg\/µL (30 fg\/µL) and a stock at 3000 pg\/µL; actual LOD may depend on instrument and sample matrix.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which real-time PCR instruments are compatible?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Linegene 9600 plus. Other instruments supporting FAM\/VIC detection and ROX (if needed) may be used — consult instrument documentation for detector\/dye settings.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at –20 °C, protect light-sensitive reagents from light. Under recommended storage the kit is stable for up to 24 months; always verify the expiration date on labels.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How many reactions does the kit support and how are reactions prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 4 × 100 reactions. Typical reaction setup is 20 µL qPCR MIX + 10 µL sample = 30 µL total. Prepare separate qPCR mixes for each fragment assay (95\/110\/215\/523) as described in the protocol.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175424188597,"sku":"1103170-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Size_Analysis_Kit_2G.png?v=1742290344"},{"product_id":"residual-e-coli-dna-size-analysis-kit-2g-shentek®","title":"Residual E. coli DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n   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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E.coli DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) kit for rapid and specific quantitation of residual Escherichia coli (E.coli) host-cell DNA across different fragment sizes during biopharmaceutical development and manufacturing. The kit amplifies four target fragment lengths (85 bp, 103 bp, 220 bp and 550 bp) using FAM-labeled probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. It supports absolute quantitation via user-prepared standard curves prepared from the supplied E.coli DNA Control and DNA Dilution Buffer (DDB). The kit is supplied as ready-to-use primer\/probe mixes and reaction buffer for high-throughput testing and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). For extraction\/preparation of host-cell DNA prior to qPCR, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 103 bp, 220 bp, 550 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eQuantitation range (standards provided)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSerial standard curve points prepared from E.coli DNA Control: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImplied sensitivity \/ lowest standard\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eLowest standard = 0.03 pg\/µL (30 fg\/µL); kit described as detecting at the femtogram (fg) level\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eqPCR MIX per well: 20 µL; add 10 µL sample\/standard\/NTC\/NCS for a total reaction volume of 30 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Instrument reads fluorescence during extension step.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFluorescent channels \/ detectors\u003c\/td\u003e\r\n                        \u003ctd\u003eTargets (E.coli fragments) - FAM; IPC - VIC; Passive reference - ROX\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve \/ analysis settings (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eUse Absolute Quantitation (Standard Curve). Analysis settings example: Manual Ct; Threshold = 0.02; Automatic baseline. Verify slope, intercept and R2 values.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of NCS Ct-IPC. Significant increase indicates possible inhibition.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eCt of NTC and NCS should be larger than mean Ct of lowest standard or show no significant amplification in FAM channel while showing normal VIC signal.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eShelf life\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components: up to 24 months (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 and other real-time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment-size analysis (four assays):\u003c\/strong\u003e Designed to quantify four discrete E.coli DNA fragment lengths (85, 103, 220 and 550 bp) to determine size distribution of residual host DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection capability down to femtogram (fg) level; lowest provided standard 0.03 pg\/µL (30 fg\/µL).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Primer \u0026amp; Probe MIX for each fragment (85, 103, 220, 550) simplifies setup and reduces handling error.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC (VIC) included to monitor inhibition and sample integrity; IPC acceptance criteria provided.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatibility and throughput:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms and formatted for 96-well plates; reagents sufficient for 4 × 100 reactions (high-throughput).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Supplied E.coli DNA Control and dilution scheme enables generation of standard curves for absolute quantitation.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual E.coli host-cell DNA in biopharmaceutical process and final products\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of host DNA clearance during process development\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and characterization of DNA fragment size distribution in purified biologics\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and assay development for host-cell DNA assessment (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli DNA Control (NNA002) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-85 (NNC110) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-103 (NNC111) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-220 (NNC112) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-550 (NNC113) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (after thaw, store at 2–8 °C; if cloudy heat to 37 °C until clear)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 400 reactions (4 × 100) | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate personal protective equipment: eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent contamination: use DNase-free, low-retention consumables; irradiate work surfaces and pipettes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment length (four assays).\u003c\/p\u003e\r\n                    \u003cp\u003e• qPCR reaction setup: prepare qPCR MIX (20 µL) and add 10 µL sample\/standard for 30 µL total reaction volume.\u003c\/p\u003e\r\n                    \u003cp\u003e• Analysis guidance: use Manual Ct, Threshold = 0.02 (example); verify slope, intercept and R2 of standard curve.\u003c\/p\u003e\r\n                    \u003cp\u003e• IPC acceptance: mean Ct-IPC for sample should be within ±1.0 Ct of NCS Ct-IPC; elevated IPC Ct suggests inhibition.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC\/NCS should show no significant amplification in FAM channel or Ct greater than lowest standard; VIC signal should be present for IPC.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use to quantify residual E.coli host-cell DNA and determine fragment size distribution in biopharmaceutical samples (in-process and final products). It is RUO and not for clinical diagnostic use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instrument channels should I use for the assay?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse FAM channel(s) for E.coli target fragment detection, VIC for the internal positive control (IPC), and ROX as the passive reference according to your instrument configuration.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the lowest concentration the kit can detect?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). The kit description indicates detection at the femtogram level; actual limit of detection should be confirmed during in-house validation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standards and controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThaw E.coli DNA Control and DDB at 2–8 °C or on ice. Dilute DNA Control to ST0 = 3000 pg\/µL then prepare serial dilutions to produce ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentrations, 1 NTC and 1 NCS per run; run samples in triplicate when possible.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175438213301,"sku":"1103171-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Size_Analysis_Kit_2G.png?v=1742290413"},{"product_id":"residual-mdck-dna-size-analysis-kit-shentek®","title":"Residual MDCK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Size Analysis Kit is designed to quantify fragment sizes of residual MDCK host cell DNA in biopharmaceutical samples across process stages (in‑process to final products). The kit utilizes a real‑time PCR (qPCR) approach to rapidly and specifically quantify MDCK DNA fragments using FAM‑labeled probes. It amplifies four discrete fragment lengths (84 bp, 142 bp, 204 bp and 504 bp) to determine size distribution with a limit of detection in the femtogram (fg) range. An internal positive control (IPC, VIC) is included to monitor inhibition and assay performance. For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). Reagents supplied support 4 × 100 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e84 bp, 142 bp, 204 bp, 504 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003efemtogram (fg) level\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR reaction volume and template input\u003c\/td\u003e\r\n                        \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template\/sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; MDCK Primer \u0026amp; Probe MIX 2.8 μL (per target); IPC MIX 1.3 μL; total 20 μL qPCR MIX\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions (example \/ ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30 (read fluorescence); 40 cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eMean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC. If significantly higher, sample may be inhibitory. NTC Ct should be ≥ 35 or undetermined.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ total reactions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions total)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eFour discrete fragment assays:\u003c\/strong\u003e Simultaneous\/parallel assays for 84 bp, 142 bp, 204 bp and 504 bp fragments for size distribution profiling.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection at the femtogram level for detection of low‑abundance residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eqPCR based, rapid and specific:\u003c\/strong\u003e Real‑time PCR with FAM probe detection provides rapid and specific quantitation of MDCK residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal inhibition control (IPC):\u003c\/strong\u003e VIC‑labeled IPC included to monitor sample inhibition and assay performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use primer\/probe mixes:\u003c\/strong\u003e Preformulated MDCK Primer \u0026amp; Probe MIXes for each fragment length reduce setup time and variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for SHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process intermediates and final products\u003c\/li\u003e\r\n                    \u003cli\u003eDetermination of host cell DNA fragment size distribution for process development and validation\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing for residual host cell DNA in vaccine and biologics manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and sample inhibition assessment using IPC\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK DNA Control (NNA006) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑84 (NNC025) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑142 (NNC026) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑204 (NNC027) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑504 (NNC028) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC070) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment length (84, 142, 204, 504 bp).\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five concentrations for the standard curve and run samples in triplicate when possible.\u003c\/p\u003e\r\n                    \u003cp\u003e• IPC criteria: mean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC; if higher, sample may be inhibitory — consider sample recovery testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC criterion: Ct for NTC should be ≥ 35 cycles or undetermined.\u003c\/p\u003e\r\n                    \u003cp\u003e• Seal plates properly, mix and spin samples before running.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components as labeled and check expiration dates before use.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103175.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103175.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit contains primer\/probe mixes for four fragment sizes: 84 bp, 142 bp, 204 bp and 504 bp.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL qPCR MIX + 10 μL template\/sample). Prepare qPCR MIX according to the protocol tables for each fragment assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How sensitive is the assay and what standards should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has a limit of detection at the femtogram level. Prepare the supplied MDCK DNA Control serial dilutions (examples: ST0 3000 pg\/μL down to ST6 0.003 pg\/μL) and include at least five standard concentrations for the standard curve.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibitory?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMonitor the IPC (VIC) signal. The mean Ct‑IPC for a sample should be within ±1.0 of the NCS Ct‑IPC. A significantly higher Ct‑IPC indicates potential inhibition and you should evaluate sample recovery or dilution.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175564304565,"sku":"1103175","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Size_Analysis_Kit.png?v=1742291658"},{"product_id":"residual-sf9-residual-dna-size-analysis-kit-shentek®","title":"Residual Sf9 Residual DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9 DNA Size Analysis Kit is a qPCR-based kit for rapid, specific quantitation and size distribution analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (from in‑process to final products). The assay amplifies four target fragment lengths (87 bp, 118 bp, 225 bp and 500 bp) using FAM-labeled detection and includes an internal positive control (IPC, VIC). The kit achieves sensitivity at the level of femtograms (fg). This package contains reagents to perform 4 × 100 reactions (primer\/probe mixes for four fragment sizes, qPCR reaction buffer, Sf9 DNA control, IPC mix and DNA dilution buffer). For DNA extraction\/sample preparation refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e87 bp, 118 bp, 225 bp, 500 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSensitivity at femtogram (fg) level\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (recommended)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, ST6 = 0.003 pg\/µL (Note: MIX-87 and MIX-118: ST1–ST6; MIX-225 and MIX-500: ST1–ST5)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR MIX per well = 20 µL; template\/sample = 10 µL; total reaction volume = 30 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9 Primer\u0026amp;Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling profile\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles (instrument reads fluorescence during extension step)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCt and analysis thresholds\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct threshold = 0.02 (FAM); VIC (IPC) threshold = 0.1; Automatic baseline recommended\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification; sample Ct-IPC should be within ±1.0 Ct of NCS Ct-IPC (IPC used to detect inhibition)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReplicates\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommend triplicates for standards, controls and test samples\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility \/ instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eValidated on SHENTEK-96S, ABI 7500, and LightCycler 480 II; compatible with other real-time PCR systems (follow instrument-specific setup)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eComponents stable for up to 24 months when stored as indicated on labels\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitative size analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (research applications)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eFour-size multiplexed analysis (separate assays):\u003c\/strong\u003e Designed to determine size distribution by amplifying four discrete fragment lengths (87, 118, 225, 500 bp) for accurate fragment-size profiling.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LOD at the femtogram level enables detection and quantitation of trace residual Sf9 DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Standard-curve (absolute quantitation) workflow with serial dilutions and recommended concentration points for accurate quantification.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC (VIC channel) included to monitor reaction integrity and identify sample inhibition.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Sf9 Primer \u0026amp; Probe MIXes for each fragment length reduce setup variability and save time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, LightCycler 480 II); protocol includes instrument-specific guidance.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual Sf9 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eResidual DNA analysis in final biologic drug products derived from Sf9\/baculovirus systems\u003c\/li\u003e\r\n                    \u003cli\u003eSize distribution profiling of host-cell DNA fragments (87–500 bp range)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and routine QC for host DNA clearance processes (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 DNA Control (NNA033), 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB002), 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-87 (NNC060), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-118 (NNC061), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-225 (NNC062), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-500 (NNC063), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069), 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB store at 2–8 °C after thawing)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat, and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surface, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at temperatures indicated on labels; kit stable up to 24 months.\u003c\/p\u003e\r\n                    \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear; store unused DDB at 2–8 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment length; include at least five standard points.\u003c\/p\u003e\r\n                    \u003cp\u003e• Run technical replicates (triplicates recommended) and include NTC and NCS controls.\u003c\/p\u003e\r\n                    \u003cp\u003e• Interpret IPC Ct: sample mean Ct-IPC should be within ±1.0 of NCS Ct-IPC; higher IPC Ct indicates inhibition.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103177.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103177.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume and reaction setup should I use for each well?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse 20 µL qPCR MIX plus 10 µL of template (purified sample, NCS or standard) for a total reaction volume of 30 µL per well.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which standard concentrations are recommended for the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse the recommended standard series: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, and ST6 = 0.003 pg\/µL. Note: use ST1–ST6 for MIX-87 and MIX-118; use ST1–ST5 for MIX-225 and MIX-500.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I know if my sample contains PCR inhibitors?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (VIC) Ct value: the sample mean Ct-IPC should be within ±1.0 Ct of the NCS Ct-IPC. A significantly higher IPC Ct in the sample indicates possible inhibition; consider dilution or additional purification and retest.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15; Annealing 60 °C for 00:30; Extension 72 °C for 01:30; 40 cycles. Instrument reads fluorescence during the extension step.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175577018549,"sku":"1103177","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_Residual_DNA_Size_Analysis_Kit.png?v=1742292573"},{"product_id":"residual-human-dna-size-analysis-kit-2g-shentek®","title":"Residual Human DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n  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14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Human DNA Size Analysis Kit (2G) is designed for the rapid and specific quantitation of residual human DNA fragments of different sizes in biopharmaceutical samples (from in‑process to final products) using real‑time PCR (qPCR). The assay amplifies four distinct fragment lengths (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer \u0026amp; probe mixes for each fragment, an internal positive control (IPC), qPCR Reaction Buffer, DNA dilution buffer and a Human DNA Control for standard curve generation. For DNA extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). This product is intended for Research Use Only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFragment lengths amplified\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; standard curve down to 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction setup (mix + template)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR mix per reaction = 20 µL (15.9 µL qPCR Reaction Buffer + 2.8 µL Primer\u0026amp;Probe Mix + 1.3 µL IPC MIX) + 10 µL template = 30 µL total reaction volume\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCt threshold guidance\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct thresholds: Human-75 = 0.04; Human-122\/244\/562 = 0.02. IPC Ct of sample should be within ±1.0 of NCS.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stable up to 24 months (check label expiration dates)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A and other real-time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment analysis:\u003c\/strong\u003e Assays four discrete human DNA fragment sizes (75, 122, 244 and 562 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram range with standard curve points to 0.03 pg\/µL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment and IPC MIX to simplify assay setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQC-focused workflow:\u003c\/strong\u003e Includes Human DNA Control for standard curves, NTC and NCS recommendations, and IPC monitoring for inhibition\/recovery.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms and configurable for different software\/instruments.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual human DNA in biopharmaceutical products (in-process to final product)\u003c\/li\u003e\r\n                    \u003cli\u003eHost cell \/ residual DNA monitoring for process development and quality control\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and release testing workflows for biologics\u003c\/li\u003e\r\n                    \u003cli\u003eComparative fragment size distribution analysis of residual DNA\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-75 (NNC008) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-122 (NNC009) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-244 (NNC010) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-562 (NNC011) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069) 550 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHuman DNA Control (NNA003) 50 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL ×3\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n                    \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment length (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five standard points, NTC and NCS; run samples in triplicate when possible.\u003c\/p\u003e\r\n                    \u003cp\u003e• IPC Ct of sample should be within ±1.0 of NCS; significantly higher IPC Ct suggests assay inhibition.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC and NCS Ct values should be larger than mean Ct of the lowest standard concentration in the FAM channel.\u003c\/p\u003e\r\n                    \u003cp\u003e• Configure analysis parameters and thresholds according to instrument model and software version.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103173.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103173.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the kit and reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore primer\/probe mixes, IPC MIX, Human DNA Control and qPCR Reaction Buffer at -20 °C. Store DNA Dilution Buffer (DDB) at 2–8 °C. The kit components are stable up to 24 months; verify expiration dates on labels.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve should I use for quantitation?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 5‑point standard curve for each fragment using the provided Human DNA Control serial dilutions: 300, 30, 3, 0.3 and 0.03 pg\/µL (ST1–ST5). Include NTC and NCS controls and run standards in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions and reaction volume?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR mix + 10 µL template). Thermal cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30 with fluorescence read during extension.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the IPC Ct indicates inhibition?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIf IPC Ct for a sample is significantly higher than the NCS (outside ±1.0), suspect inhibition. Consider re‑extracting or diluting the sample, check sample recovery (if ERC included), and reassess prior to reporting.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175607951541,"sku":"1103173","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Size_Analysis_Kit_2G.jpg?v=1742292680"},{"product_id":"residual-bhk-dna-size-analysis-kit-shentek®","title":"Residual BHK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Size Analysis Kit is designed for quantitative measurement of residual BHK host-cell DNA fragments across different fragment sizes in biopharmaceutical samples (from in-process samples to final products). The assay uses quantitative PCR with FAM-labeled probes to amplify four discrete fragment sizes (81 bp, 134 bp, 216 bp and 589 bp) to determine size distribution and quantity. The kit provides high sensitivity with a reported limit of detection (LOD) at the femtogram (fg) level. An internal positive control (IPC, VIC channel) is included to monitor inhibition and extraction\/reaction performance. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e81 bp, 134 bp, 216 bp, 589 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eFemtogram (fg) level sensitivity (LOD stated as fg level)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (per kit instructions)\u003c\/td\u003e\r\n                        \u003ctd\u003eStandards used (ST1-ST5): 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (serial dilutions prepared from ST0 source)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR reaction volume: 30 µL total (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; BHK Primer \u0026amp; Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL qPCR MIX\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Fluorescence is read during the extension step.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n                        \u003ctd\u003eTarget probes: FAM reporter for each BHK fragment assay; IPC: VIC reporter. ROX recommended as passive reference (ABI instruments).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNTC \/ NCS acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be ≥ 35 cycles; NCS Ct should be greater than mean Ct of the lowest standard and show normal VIC amplification. IPC Ct of sample should be within ±1.0 of NCS IPC Ct; deviations indicate inhibition.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecovery acceptance\u003c\/td\u003e\r\n                        \u003ctd\u003eSample recovery rate (ERC-based) should be between 50% and 150%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (stated in product label \/ packaging)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLOQ\u003c\/td\u003e\r\n                        \u003ctd\u003eLimit of quantitation (LOQ) not numerically specified in the user guide; users should refer to assay validation for LOQ\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMulti‑size quantitation:\u003c\/strong\u003e Simultaneous assessment of residual BHK DNA across four discrete fragment sizes (81, 134, 216, 589 bp) to provide size distribution information.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity reaches femtogram (fg) level LOD for detection of low-abundance residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eqPCR-based specificity:\u003c\/strong\u003e TaqMan-style primer\/probe assays (FAM) deliver rapid and specific quantitation; IPC (VIC) monitors inhibition and reaction integrity.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe MIXes simplify assay setup and reproducibility.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (examples: SHENTEK-96S, ABI 7500, Roche LightCycler 480) and configurable for other real-time PCR systems.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual BHK host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n                    \u003cli\u003eSize-distribution analysis of residual DNA in in-process and final drug product samples\u003c\/li\u003e\r\n                    \u003cli\u003eMethod development and validation for host-cell DNA monitoring\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing during upstream\/downstream processing and release testing (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBHK DNA Control (NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-81 (NNC072) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-134 (NNC073) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-216 (NNC074) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-589 (NNC075) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC070) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 total reactions, as stated on product label) | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (see compliance). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C (on ice), vortex and briefly centrifuge before use; protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare standard curves (minimum five concentrations recommended) and run samples in triplicate when possible.\u003c\/p\u003e\r\n                    \u003cp\u003e• qPCR reaction setup: 20 µL qPCR MIX + 10 µL sample\/standard = 30 µL total per well; seal plates and centrifuge briefly prior to run.\u003c\/p\u003e\r\n                    \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35; IPC Ct of sample should be within ±1.0 of NCS IPC Ct; recovery rates should be between 50% and 150%.\u003c\/p\u003e\r\n                    \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear and store remaining DDB at 2–8 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Limit of quantitation (LOQ) must be established\/validated by user; if LOQ is lower than lowest standard, NCS Ct should be below LOQ concentration.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103179.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103179.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect and quantify?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit amplifies and quantifies four discrete BHK host‑cell DNA fragment sizes: 81 bp, 134 bp, 216 bp and 589 bp.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LOD) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports a limit of detection (LOD) at the femtogram (fg) level. Exact LOD\/LOQ values should be confirmed during in-house validation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What reaction volume and thermal cycling program should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30. Fluorescence is read during the extension step.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC, NTC and NCS results?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles. IPC Ct of a sample should be within ±1.0 of the NCS IPC Ct; a higher IPC Ct indicates potential inhibition. NCS Ct should be greater than the mean Ct of the lowest standard and exhibit normal VIC amplification. Recovery rate (ERC-based) should be 50–150%.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175635017909,"sku":"1103179","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Size_Analysis_Kit.jpg?v=1742292816"},{"product_id":"residual-pg13-dna-size-analysis-kit-shentek®","title":"Residual PG13 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual PG13 DNA Size Analysis Kit is designed to quantify residual PG13 host-cell DNA across different fragment sizes during biopharmaceutical production (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (FAM) assay to rapidly and specifically measure PG13 DNA fragments. The assay amplifies three discrete target fragment sizes (118 bp, 212 bp and 502 bp) to determine size distribution. The kit includes an internal positive control (IPC) and a PG13 DNA control for generation of absolute-quantitation standard curves. Limit of detection (LOD) reaches the femtogram (fg) level. For DNA extraction recommendations, refer to the companion SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e118 bp, 212 bp, 502 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eLOD reaches femtogram (fg) level\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR MIX composition (per 1 reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; PG13 Primer\u0026amp;Probe MIX (per target) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30 (read fluorescence) — 40 cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument\/readout channels\u003c\/td\u003e\r\n                        \u003ctd\u003eFAM for PG13 targets; VIC for IPC; ROX used as passive reference (ABI7500 example)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eMean Ct(IPC) for sample should be within ±1.0 of NCS Ct(IPC); a significantly higher Ct indicates possible inhibition\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecovery rate (ERC)\u003c\/td\u003e\r\n                        \u003ctd\u003eExpected ERC recovery between 50% and 150%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNTC and NCS criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be ≥35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n                        \u003ctd\u003eComponents stored at –20 °C; stable for up to 24 months (check label expiration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProvided reagent format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 3 × 100 reactions (stated on product cover)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Three separate primer\/probe assays (118 bp, 212 bp, 502 bp) enable determination of residual DNA size distribution, not just total DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay LOD reaches femtogram (fg) levels for detection of trace residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA control and recommended serial dilution scheme to build standard curves for absolute quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition monitoring:\u003c\/strong\u003e IPC (VIC) included to monitor PCR inhibition and assay performance on a per-sample basis.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, LightCycler 480).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe mixes reduce setup time and variability.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual PG13 host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of DNA fragmentation during downstream processing\u003c\/li\u003e\r\n                    \u003cli\u003eFinal product quality control and release testing for residual host-cell DNA\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and process development for gene therapy\/viral vector production using PG13-derived material\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePG13 DNA Control (NNA049) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 µL × 6 tubes (protect from light)\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-118 (NNC083) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-212 (NNC084) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-502 (NNC085) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC070) — 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 3 × 100 reactions (300 reactions) | Method: qPCR (fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n                    \u003cp\u003e• Work in a clean, DNase-free environment; decontaminate surfaces and equipment (UV 30 minutes and 75% ethanol recommended).\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and brief-spin before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store components at –20 °C; check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitate, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include appropriate controls: at least 5 standard points, NTC, and NCS; recommended technical triplicates for samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• IPC performance: sample mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant deviation may indicate inhibition.\u003c\/p\u003e\r\n                    \u003cp\u003e• ERC (extraction recovery control) recovery should be 50%–150%; investigate out-of-range recoveries.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC Ct should be ≥35 cycles or undetermined; NCS should be greater than lowest standard Ct and show VIC amplification.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103178.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103178.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms, including SHENTEK-96S, ABI 7500 series, and LightCycler 480. If using a different instrument, follow the instrument\/software documentation for detector and plate setup.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThaw PG13 DNA Control and DDB at 2–8 °C. Dilute the DNA Control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five standards in the standard curve and run in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are acceptable IPC and recovery criteria?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct(IPC) for a sample should be within ±1.0 of the NCS Ct(IPC). ERC recovery rates should fall between 50% and 150%; values outside this range warrant investigation for extraction efficiency or inhibition.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if my DNA Dilution Buffer is cloudy?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIf DDB is cloudy or contains precipitate, heat the buffer at 37 °C until it becomes clear, then cool and use. Store DDB at 2–8 °C after opening or per kit instructions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"3×100 Reactions","offer_id":45175694983349,"sku":"1103178","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Size_Analysis_Kit.jpg?v=1742292887"},{"product_id":"residual-hpv18-e6-e7-dna-size-analysis-kit-shentek®","title":"Residual HPV18 E6\/E7 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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#c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 1 hour\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HPV18 E6\/E7 DNA Size Analysis Kit is a real-time PCR (qPCR) kit designed for rapid and specific quantitation of residual HPV18 E6 and E7 DNA fragments of different sizes (from HeLa cell origin) across biopharmaceutical sample types (in-process to final products). The kit amplifies four fragment lengths (E6: 100 bp and 288 bp; E7: 110 bp and 240 bp) using FAM-labeled target probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. The kit includes HPV E6\/E7 DNA Control for standard curve generation and is intended for research use only. For sample extraction instructions refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget genes \/ fragments\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHPV18 E6 (100 bp, 288 bp) and E7 (110 bp, 240 bp)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection chemistry \/ channels\u003c\/td\u003e\r\n                        \u003ctd\u003eFAM-labeled probes for target fragments; VIC-labeled probe for IPC; ROX as passive reference (recommended)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample or standard)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 17 μL + primer\u0026amp;probe mix 3 μL (per target assay); IPC qPCR mix: qPCR Reaction Buffer 15.9 μL, IPC MIX 1.3 μL, ddH2O 2.8 μL (per 20 μL qPCR MIX)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during annealing\/extension)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve \/ dynamic range (provided example)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStandard curve points shown: 1×10^8 to 1×10^2 copies\/μL (ST0 = 1×10^8, ST1 = 1×10^7 ... ST6 = 1×10^2); at least five concentrations recommended\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReplicates\u003c\/td\u003e\r\n                        \u003ctd\u003eTriplicates recommended for standards, controls and test samples\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls included \/ required\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHPV E6\/E7 DNA Control (quantified) for standard curve; NTC (no template control); NCS (negative control sample); IPC for inhibition monitoring\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInput\/sample volume\u003c\/td\u003e\r\n                        \u003ctd\u003e10 μL purified sample or 10 μL standard per reaction\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (as labeled on product)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eShelf life\u003c\/td\u003e\r\n                        \u003ctd\u003eUp to 24 months when stored at –20 °C (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNot specified in the user guide; recommended to determine during in-house validation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInhibition acceptance criterion\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMean Ct of IPC for sample should be within ±1.0 of the NCS Ct-IPC; significant increase suggests inhibition and sample recovery testing is recommended\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus and other real-time PCR systems (user must configure detectors\/filters accordingly)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eFragment-size specific quantitation:\u003c\/strong\u003e Designed to amplify and quantify four distinct HPV18 E6\/E7 fragment sizes (E6:100 bp \u0026amp; 288 bp; E7:110 bp \u0026amp; 240 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReal-time qPCR with internal control:\u003c\/strong\u003e Uses FAM-labeled probes for targets and VIC-labeled IPC to monitor inhibition and assay performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-formulated primer \u0026amp; probe mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment simplify setup and reduce pipetting errors; probes are light-sensitive (protect from light).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use standards:\u003c\/strong\u003e Includes quantified HPV E6\/E7 DNA Control and detailed serial dilution protocol for building standard curves and absolute quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Provides recommended plate layouts, qPCR conditions, and data analysis settings for ABI7500 (can be adapted to other instruments).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual HPV18 E6\/E7 DNA (HeLa origin) in biopharmaceutical production\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring and final product quality control for residual host cell DNA (HPV18-specific)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and assay development for residual DNA size-distribution analysis\u003c\/li\u003e\r\n                    \u003cli\u003eSample inhibition assessment and recovery evaluation using IPC\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX\u003c\/span\u003e\u003cstrong\u003e150 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE6-100 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE6-288 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE7-110 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE7-240 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e850 μL × multiple tubes (as supplied); –20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB, NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL ×2 tubes; store at 2–8 °C (if cloudy, heat to 37 °C to clarify)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHPV E6\/E7 DNA Control (NNA030)\u003c\/span\u003e\u003cstrong\u003e50 μL ×1 tube; –20 °C (check label for concentration before dilution)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR (real-time PCR, absolute quantitation using standard curve)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect primer \u0026amp; probe mixes and IPC MIX from light; store kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes (UV 30 minutes recommended) and use dedicated pre- and post-PCR areas to minimize contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare and run NTC, NCS and IPC controls as instructed. NCS must be processed the same as test samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• If IPC Ct for a sample is \u0026gt; ±1.0 compared to NCS IPC Ct, the sample may contain inhibitors; perform recovery testing or further sample cleanup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow instrument-specific software settings and thresholds; example settings provided for ABI 7500 (Manual Ct, threshold 0.02, automatic baseline).\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the expected assay runtime?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe qPCR program provided runs activation 95 °C for 10 min plus 40 cycles of 95 °C for 15 s and 60 °C for 60 s, resulting in an approximate total runtime of ~1 hour per run.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the supplied HPV E6\/E7 DNA Control, dilute to prepare ST0 at 1×10^8 copies\/μL, then perform serial 1:10 dilutions to generate ST1–ST6 (1×10^7 to 1×10^2 copies\/μL). Include at least five concentrations across the dynamic range and run in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if the IPC indicates inhibition?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample mean Ct-IPC is significantly higher than NCS Ct-IPC (\u0026gt;±1.0), the sample may be inhibitory. Perform recovery testing (ERC) in the same assay, consider additional sample cleanup or dilution, and re-test.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What is the assay sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLOD\/LOQ are not specified in the user guide. Determine LOD\/LOQ, precision and accuracy during in-house validation using the provided standard curve and appropriate validation procedures.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Products are intended for research use only.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175697965237,"sku":"SK030307S-P","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HPV18_E6_E7_DNA_Size_Analysis_Kit.jpg?v=1742292960"},{"product_id":"virus-dna-rna-extraction-kit-shentek®","title":"Virus DNA \u0026 RNA Extraction Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eNucleic acid extraction\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eVarious: 2-8 °C, -20 °C, RT (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Virus DNA \u0026amp; RNA Extraction Kit is a magnetic-particle based kit designed for isolation of viral DNA and RNA from cell samples (up to 10^7 cells) and harvested virus bulk (liquid). The kit supports both manual workflows and automated extraction with the SHENTEK® rHCDpurify system. Two sample workflows are provided: Method 1 (for viruses replicated in host nucleus or unknown viruses) includes an optional nuclease digestion step after lysis to reduce background nucleic acids; Method 2 (for viruses replicated in host cytoplasm) uses lysis followed by centrifugation and extraction of the supernatant. The kit contains protease digestion, binding, wash and elution reagents and magnetic particles to deliver purified nucleic acids suitable for downstream molecular assays (e.g., PCR\/qPCR, sequencing). The kit is supplied for 50 extractions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePack size\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number\u003c\/td\u003e\r\n                        \u003ctd\u003e1506730\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input capacity\u003c\/td\u003e\r\n                        \u003ctd\u003eUp to 1 × 10^7 cells; maximum sample volume per well up to 500 µL (automated plate format)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eEluate volume\u003c\/td\u003e\r\n                        \u003ctd\u003e50–100 µL RNase-free H2O (preheated at 70 °C); ensure \u0026gt;40 µL for downstream assay\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\r\n                        \u003ctd\u003e200 µL binding buffer + 200–400 µL isopropanol + 30 µL magnetic particles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n                        \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKey incubation temperatures \u0026amp; times\u003c\/td\u003e\r\n                        \u003ctd\u003eProtease digestion: 55 °C for 30 min; Nuclease digestion (optional): 37 °C for 30 min; Elution: 70 °C for 7 min; Magnetic particles incubation at RT 10 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\r\n                        \u003ctd\u003eIncubate magnetic particles at RT 10 min and vortex to mix before use; aliquot to avoid repeated temperature shifts\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMaximum sample volume (plate)\u003c\/td\u003e\r\n                        \u003ctd\u003eMaximum per deep-well plate well: 500 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months (see individual component storage below)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eManual extraction or automated extraction using SHENTEK® rHCDpurify system; downstream compatible with PCR\/qPCR and sequencing workflows\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDual DNA \u0026amp; RNA extraction:\u003c\/strong\u003e Simultaneous isolation of both viral DNA and RNA from the same sample using magnetic-particle chemistry.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eTwo-method workflow:\u003c\/strong\u003e Method 1 includes nuclease digestion for nucleus-replicating or unknown viruses to reduce background; Method 2 optimized for cytoplasm-replicating viruses (centrifugation of lysate).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and automated operation:\u003c\/strong\u003e Validated for manual tube-based workflows and automated high-throughput extraction with the SHENTEK® rHCDpurify system (96-deep well plate layout supported).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic particle technology:\u003c\/strong\u003e Magnetic beads provide reliable binding, washing and elution for high-quality nucleic acids suitable for downstream molecular assays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady for downstream molecular assays:\u003c\/strong\u003e Eluted nucleic acids are suitable for PCR\/qPCR and sequencing; recommended to perform detection assays the same day for best accuracy.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit reagents stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of viral DNA and RNA from cell culture samples (up to 1×10^7 cells)\u003c\/li\u003e\r\n                    \u003cli\u003eExtraction of nucleic acids from harvested virus bulk (liquid)\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for PCR and qPCR assays\u003c\/li\u003e\r\n                    \u003cli\u003ePreparation of viral nucleic acids for sequencing or downstream molecular testing\u003c\/li\u003e\r\n                    \u003cli\u003eProcessing of rcAAV\/rAAV, replication competent lentivirus (RCL), replication competent retrovirus (RCR) and RNA viruses\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (15 mL × 1 bottle)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (10 mL × 1 bottle)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND025)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (5 mL × 1 bottle)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePretreatment buffer (NND002)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (1.25 mL × 2 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2–8 °C (750 µL × 2 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (500 µL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePrecipitation solution I (NND003)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (25 µL × 1 tube) — to be diluted 1:99 with RNase-Free H2O before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.2 mL × 6 bottles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003eStore per label (500 µL × 5 tubes) — follow kit label\/insert\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic bead-based nucleic acid extraction (manual or automated with rHCDpurify)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eye protection, masks, gloves, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Separate laboratory areas for reagent preparation, sample preparation and amplification to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure ambient temperature ≥ 22 °C at experiment start.\u003c\/p\u003e\r\n                    \u003cp\u003e• Change gloves regularly and use dedicated protective equipment for different lab areas.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge tubes before opening to avoid aerosols; spin down liquid on tube caps\/walls.\u003c\/p\u003e\r\n                    \u003cp\u003e• Discard and disinfect liquid waste and used tips according to lab biosafety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not over-dry magnetic particle pellet; over-drying may hinder resuspension during elution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Place tubes on magnetic stand with pellet oriented toward the magnet and rotate tubes slowly to accelerate bead aggregation.\u003c\/p\u003e\r\n                    \u003cp\u003e• After extraction, perform DNA\/RNA detection assays preferably the same day for accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• For automated runs with rHCDpurify: perform UV sterilization for at least 15 minutes and wipe interior with 75% ethanol before and after runs; maintain at least 30 min between extractions.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506730.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506730.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Can this kit isolate both viral DNA and RNA?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit is formulated to extract both viral DNA and RNA from cell samples and harvested virus liquid.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many samples can be processed with one kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe supplied kit contains reagents for 50 extractions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is the kit compatible with automation?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the SHENTEK® rHCDpurify system (96-deep well plate format).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What elution volume and conditions are recommended?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL RNase-Free H2O preheated to 70 °C and incubate at 70 °C for 7 minutes; ensure at least ~40 µL final elute for downstream assays.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45181418963125,"sku":"1506730","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SHENTEK_Virus_DNA_RNA_Extraction_Kit.jpg?v=1742346294"},{"product_id":"replication-competent-lentivirus-rcl-quantitation-kit-shentek®","title":"Replication-Competent Lentivirus (RCL) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Replication-Competent Lentivirus (RCL) Quantitation Kit is used for the quantitative detection of replicable lentiviral RCL in cell therapy products and gene therapy products produced with lentiviral vectors, such as virus-producing cell banks, end of production cells, viral vectors and CAR-T cells. This kit is rapid, specific and reliable, and can work in coordination with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit to quantitate the copy number of replicable lentivirus RCL in samples.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent lentivirus (RCL) in cell therapy and gene therapy products (virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eOne-step reverse transcription quantitative PCR (qRT-PCR) with FAM-labeled probe; ROX passive reference recommended.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProvided standard curve range (recommended)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStandard dilutions ST2–ST6: 6 × 10^5, 6 × 10^4, 6 × 10^3, 6 × 10^2, 6 × 10^1 copies\/µL (prepared from ST = 6 × 10^8 copies\/µL).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended reaction volumes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eqRT-PCR MIX per reaction 10 µL; add 20 µL sample\/standard\/NTC for a total reaction volume of 30 µL.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqRT-PCR master mix composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003e5×RT-qPCR Buffer 6 µL; RT-qPCR Enzyme MIX 1 µL; RCL Primer\u0026amp;Probe MIX 3 µL; total 10 µL.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 0:30 (1 cycle); 40 cycles: 95 °C 0:15 (denature), 60 °C 1:00 (anneal\/extend, fluorescence read).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, Applied Biosystems 7500, LightCycler 480 II, CFX96, qTOWER3 G, etc.).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection\/read settings\u003c\/td\u003e\r\n                        \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Threshold setting example: 0.02 (manual Ct), automatic baseline.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be \u0026gt;= 35.00 cycles. NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored at -20 °C; stable for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSensitivity \/ dynamic range (inferred from standard curve)\u003c\/td\u003e\r\n                        \u003ctd\u003eDemonstrated standard curve points cover at least 6 × 10^5 to 6 × 10^1 copies\/µL (user should perform validation to determine empirical LOD\/LOQ).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReplicates recommended\u003c\/td\u003e\r\n                        \u003ctd\u003eTriplicate wells recommended for each standard, control and test sample.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input\u003c\/td\u003e\r\n                        \u003ctd\u003e20 µL purified sample per 30 µL reaction (per example workflow); samples must be processed\/extracted (e.g., with SHENTEK Virus DNA \u0026amp; RNA Extraction Kit) prior to qRT-PCR.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eRapid and specific quantitation:\u003c\/strong\u003e One-step qRT-PCR assay optimized for accurate quantitation of replication-competent lentivirus (RCL).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream sample prep.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, buffer and enzyme mixes; standardized control for construction of a quantitative standard curve.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh throughput:\u003c\/strong\u003e Reagents provided for up to 100 reactions and plate-based workflows (96-well format) supported.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eEstablished QC criteria:\u003c\/strong\u003e Includes recommended control acceptance criteria (NTC and NCS Ct thresholds) and example standard curve settings for result interpretation.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of replication-competent lentivirus (RCL) in virus-producing cell banks\u003c\/li\u003e\r\n                    \u003cli\u003eTesting end-of-production cells for RCL contamination\u003c\/li\u003e\r\n                    \u003cli\u003eQuantitation of RCL in viral vector preparations\u003c\/li\u003e\r\n                    \u003cli\u003eSafety testing of CAR-T cells and other lentiviral-transduced cell therapy products\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and release testing workflows for gene and cell therapy manufacturing (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRCL Control (Part No. NNA044), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRCL Primer\u0026amp;Probe MIX (Part No. NNC077), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5×RT-qPCR Buffer (Part No. NNC078), 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRT-qPCR Enzyme MIX (Part No. NNC079), 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase-Free H2O (Part No. NND008), 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eddH2O (Part No. NND010), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents from light; store all components at -20 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent RNase contamination: use RNase-free consumables and work areas; thaw reagents at 2–8 °C or on ice.\u003c\/p\u003e\r\n                    \u003cp\u003e• UV-decontaminate benches, micropipettes and tubes for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Process Negative Control Sample (NCS) through the same extraction and handling steps as test samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five standard curve points (ST2–ST6) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Verify instrument-specific parameter settings; follow instrument documentation when adapting program parameters.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403441.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403441.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is intended for research use to quantitatively detect replication-competent lentivirus (RCL) in cell therapy and gene therapy related samples (e.g., virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What detection instrument and dye settings should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96). Use FAM as the reporter dye and ROX as the passive reference (if applicable).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended reaction and cycling conditions?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 30 µL reaction (10 µL qRT-PCR MIX + 20 µL sample). Thermal program: 50 °C 15:00 (RT), 95 °C 0:30 (activation), then 40 cycles of 95 °C 0:15 and 60 °C 1:00 (fluorescence read).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve and what is the dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the supplied RCL control and perform serial 10-fold dilutions to generate ST2–ST6. Recommended standard concentrations span 6 × 10^5 to 6 × 10^1 copies\/µL; include at least five points for the standard curve and run in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181705552053,"sku":"1403441","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Lentivirus_RCL_Quantitation_Kit.jpg?v=1742361933"},{"product_id":"replication-competent-retrovirus-rcr-quantitation-kit-shentek®","title":"Replication-Competent Retrovirus (RCR) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    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#cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Replication-Competent Retrovirus (RCR) Quantitation Kit is designed for quantitative detection of Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products produced with retrovirus vectors. The kit supports absolute quantitation using a standard-curve qRT-PCR workflow and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream nucleic acid preparation. The kit provides lyophilized RCR control, primer \u0026amp; probe mix, qRT-PCR buffer and enzyme mix, and reagents sufficient for 100 reactions. Intended for rapid, specific and reliable monitoring of RCR in virus-producing cell banks, end-of-production cells, viral vector preparations and CAR-T cells.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by qRT-PCR using a standard curve\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended standard curve points\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eST2–ST6 (five concentration points) corresponding to 5 × 10^5, 5 × 10^4, 5 × 10^3, 5 × 10^2, 5 × 10^1 copies\/μL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControl stock concentration (after reconstitution)\u003c\/td\u003e\r\n                        \u003ctd\u003eLabelled RCR Control reconstituted to 5 × 10^8 copies\/μL then serially diluted\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSuggested quantitation range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e5 × 10^1 – 5 × 10^5 copies\/μL (based on ST6–ST2 standard points)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection \/ quantitation\u003c\/td\u003e\r\n                        \u003ctd\u003eNot explicitly specified in manual; lowest standard provided is 5 × 10^1 copies\/μL (ST6)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n                        \u003ctd\u003eqRT-PCR mix per well 10 μL + sample 20 μL = total 30 μL reaction volume\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqRT-PCR component volumes (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003e5×RT-qPCR Buffer 6 μL, RT-qPCR Enzyme MIX 1 μL, RCR Primer\u0026amp;Probe MIX 3 μL (total 10 μL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n                        \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during 60 °C step)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96, qTOWER3 G); settings provided for Applied Biosystems 7500 SDS v1.4\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of the lowest standard concentration.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at -20 °C; components stable up to 24 months (check labels for expiration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for RCR quantitation:\u003c\/strong\u003e Specifically targets Replication-Competent Retrovirus (RCR) in cell and gene therapy products, enabling product safety testing and release control.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a serially diluted lyophilized RCR control and a standard-curve qRT-PCR method to provide copy number (copies\/μL).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete qRT-PCR reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, 5× RT-qPCR buffer, RT-qPCR enzyme mix, RNase-free water and a lyophilized control; reagents provided for 100 reactions.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatible with common real-time PCR platforms:\u003c\/strong\u003e Validated instructions and program settings provided for multiple instruments (e.g., ABI 7500, LightCycler 480 II, CFX96).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eRNase-free and protected formulations:\u003c\/strong\u003e RNase-free water and light-protected mixes supplied; some components labeled 'protect from light'.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitative detection of RCR in virus-producing cell banks\u003c\/li\u003e\r\n                    \u003cli\u003eTesting end-of-production cells for RCR contamination\u003c\/li\u003e\r\n                    \u003cli\u003eQuantitation of RCR in viral vector preparations\u003c\/li\u003e\r\n                    \u003cli\u003eScreening CAR‑T or other cell therapy products for RCR\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and release testing during gene\/cell therapy manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and assay development for RCR monitoring\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRCR Control (lyophilized powder)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRCR Primer \u0026amp; Probe MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5× RT-qPCR Buffer\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRT-qPCR Enzyme MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase-Free H2O (1.2 mL × 3)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eddH2O (1 mL × 1)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR (absolute quantitation by standard curve)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Work in RNase-free conditions; use RNase-free consumables and reagents.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents from light; store at -20 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice. Do not refreeze repeatedly.\u003c\/p\u003e\r\n                    \u003cp\u003e• Dissolve lyophilized control by adding specified volume of ddH2O and mix thoroughly (vortex\/flick and centrifuge as instructed).\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surfaces and equipment (UV irradiation 30 min and 75% ethanol recommended).\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five standard curve points (recommended ST2–ST6) and appropriate controls (NTC, NCS).\u003c\/p\u003e\r\n                    \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35.00 or undetermined; NCS Ct must be larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403442.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403442.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively detect Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products, including virus-producing cell banks, end-of-production cells, viral vectors and CAR‑T cells.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions does the kit support and what is the reaction setup?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Each reaction is prepared as a 30 μL total volume (10 μL qRT-PCR mix + 20 μL sample or water), with qRT-PCR mix composed of 6 μL 5×RT-qPCR Buffer, 1 μL RT-qPCR Enzyme MIX and 3 μL RCR Primer\u0026amp;Probe MIX per reaction.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the quantitation range and lowest standard provided?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe recommended standard curve uses five points (ST2–ST6) corresponding to 5 × 10^5 to 5 × 10^1 copies\/μL. The lyophilized RCR Control is reconstituted to 5 × 10^8 copies\/μL and serially diluted to prepare standards.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What instrument settings and acceptance criteria should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThermal program: RT 50 °C 15:00; Activation 95 °C 00:30; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence at 60 °C). Use absolute quantitation (standard curve). NTC Ct should be ≥ 35 or undetermined; NCS Ct should be larger than mean Ct of the lowest standard.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181719576757,"sku":"1403442","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Retrovirus_RCR_Quantitation_Kit.jpg?v=1742362083"},{"product_id":"rcaav-2-n-quantitation-kit-shentek®","title":"rcAAV-2\/N Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® rcAAV Quantitation Kit is a qPCR-based kit for detection and quantitation of replication-competent adeno-associated virus (rcAAV) contamination in recombinant AAV (rAAV) serotypes designated as rAAV-2\/N. The kit is designed to quantify rcAAV-2\/N contamination in rAAV production samples including harvested cell-culture bulk, purified stocks and final products. The kit contains a T\u0026amp;R DNA control for preparation of an absolute quantitation standard curve, separate Target and Reference Primer \u0026amp; Probe mixes, qPCR reaction buffer and supporting reagents. The kit supports standard-curve (absolute) quantitation and is compatible with common qPCR platforms (e.g., ABI 7500, Bio-Rad CFX96, Roche 480, SHENTEK-96S).\u003c\/p\u003e\n\u003cp\u003eImportant: The ITR sequence of the rAAV test sample must match the provided ITR sequence for correct detection. Reagents supplied are sufficient for 2 × 100 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent AAV (rcAAV-2\/N) contamination in rAAV-2\/N samples by qPCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNumber of reactions (kit size)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 2 × 100 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDynamic range \/ standard curve\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e20 to 2 × 10^6 copies\/µL (standard curve concentrations: ST6=2×10^1 to ST1=2×10^6 copies\/µL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e20 copies\/µL (lowest standard used for quantitation)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL total reaction volume (10–10.04 µL qPCR mix + 20 µL sample\/standard per well)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling (example ABI 7500)\u003c\/td\u003e\r\n                        \u003ctd\u003ePre-melt 95 °C 10:00 (1 cycle); Denature 95 °C 00:15; Anneal\/extend 60 °C 00:30; Read at 72 °C 00:30; 40 cycles. (Note: ABI 7500 users set final read step to 72 °C 34 s.)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetector channels \/ probes\u003c\/td\u003e\r\n                        \u003ctd\u003eTarget-2 detected in FAM channel; Reference-2 detected in CY5 channel; separate primer\/probe mixes provided\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCt threshold settings (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eReference-2 threshold = 0.06; Target-2 threshold = 0.02; use automatic baseline and manual Ct\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCt acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct (Reference and Target) should be \u0026gt;35 or undetermined. NCS Ct values should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContamination calculation\u003c\/td\u003e\r\n                        \u003ctd\u003ercAAV contamination rate = (Detection value of Target genes) ÷ (Detection value of Reference genes × dilution factor ÷ 2). Note: each rAAV contains two copies of the Reference gene.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eABI 7500, Bio-Rad CFX96, SHENTEK-96S, Roche 480 and other real-time PCR systems (ROX usage depends on instrument)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for rcAAV-2\/N detection:\u003c\/strong\u003e Specifically optimized to quantify replication-competent AAV contamination in rAAV-2\/N serotypes using ITR-targeted assays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation by standard curve:\u003c\/strong\u003e Includes T\u0026amp;R DNA Control and dilution scheme to generate a 6-point standard curve (2×10^1 – 2×10^6 copies\/µL) for absolute quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete assay components:\u003c\/strong\u003e Provides qPCR reaction buffer, Target and Reference primer\/probe mixes, ROX option, DNA dilution buffer and DNA control for streamlined setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePlatform flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR instruments; ROX addition is optional depending on instrument model.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eProcedural controls and guidance:\u003c\/strong\u003e Includes NTC and NCS guidance, recommended plate layouts, Ct thresholds, and sample dilution recommendations to support robust QC workflows.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of rcAAV contamination in recombinant AAV production (rAAV stocks and final products)\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing during rAAV manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eAnalysis of harvested cell-culture samples for presence of replication-competent AAV\u003c\/li\u003e\r\n                    \u003cli\u003eLot release and batch testing for rcAAV contamination\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eT\u0026amp;R DNA Control-2 (lyophilized powder, 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ercAAV qPCR Reaction Buffer (400 µL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTarget Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReference Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× ROX (20 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eddH2O (1 mL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e2–8 °C (or -20 °C long-term)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 2 × 100 reactions | Method: qPCR (Absolute Quantitation by Standard Curve)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, lab coat, eye protection, mask) when handling reagents.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent nucleic acid contamination: UV-treat surfaces, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Benzonase nuclease treatment of rAAV stocks prior to extraction is required to remove non-encapsidated nucleic acids and improve direct-qPCR accuracy.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit instructions for sample extraction when applicable.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use; protect light-sensitive reagents (reaction buffer) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge the lyophilized DNA control at 12,000 rpm for 1 min and reconstitute precisely with 55 µL ddH2O before preparing dilutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare standard curve fresh (recommended six points) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 100× ROX only if required by the qPCR instrument (ABI 7500 requires ROX; SHENTEK-96S and Roche 480 generally do not).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use recommended Ct threshold settings (Reference = 0.06; Target = 0.02) and check slope\/intercept\/R² of standard curve for assay validity.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403444.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403444.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supports purified rAAV bulk, final product stocks and harvested cell-culture samples. For bulk or final product stocks, perform Benzonase treatment and nucleic acid extraction prior to qPCR. Cell culture samples may be extracted using the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay dynamic range and lowest quantifiable concentration?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe standard curve covers 2 × 10^1 to 2 × 10^6 copies\/µL (20 to 2,000,000 copies\/µL). The lowest standard used for quantitation (LOQ) is 20 copies\/µL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How is rcAAV contamination rate calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ercAAV contamination rate = (Measured copies of Target gene) ÷ (Measured copies of Reference gene × dilution factor ÷ 2). Note that each rAAV contains two copies of the Reference gene.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Do I need to add ROX to every instrument?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. Addition of 100× ROX is instrument-dependent. ABI 7500 requires 0.04 µL ROX per reaction for the Target assay; SHENTEK-96S, Roche 480 and some other systems do not require ROX. Follow your instrument documentation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). For Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions x 2","offer_id":45181732618421,"sku":"1403444","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rcAAV-2_N_Quantitation_Kit.jpg?v=1742362203"},{"product_id":"rcaav-5-n-quantitation-kit-shentek®","title":"rcAAV-5\/N Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® rcAAV Quantitation Kit is designed for qPCR detection and absolute quantitation of replication-competent adeno-associated virus (rcAAV) contamination in rAAV-5\/N serotypes. The kit is suitable for testing recombinant AAV bulk, purified end-products and harvested cell-culture samples. The assay targets the ITR sequence of rAAV-5\/N and includes T\u0026amp;R DNA control material, separate Reference and Target primer\/probe mixes, and reagents for construction of a standard curve for absolute quantitation. Benzonase treatment of rAAV prior to extraction is recommended to remove unprotected nucleic acids and improve accuracy of direct qPCR measurements.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve range (linear range)\u003c\/td\u003e\r\n                        \u003ctd\u003e20 - 2 × 10^6 copies\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard points (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 = 2 × 10^6 copies\/µL; ST2 = 2 × 10^5; ST3 = 2 × 10^4; ST4 = 2 × 10^3; ST5 = 2 × 10^2; ST6 = 2 × 10^1\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL total reaction volume (10 µL qPCR MIX + 20 µL sample\/standard\/DDB per well)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eReference: 8 µL rcAAV qPCR Reaction Buffer + 2 µL Reference Primer\u0026amp;Probe MIX-5; Target: 8 µL rcAAV qPCR Reaction Buffer + 2 µL Target Primer\u0026amp;Probe MIX-5 (+ 0.04 µL 100×ROX optional for some instruments)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling program (example \/ ABI7500)\u003c\/td\u003e\r\n                        \u003ctd\u003ePre-melt 95 °C 10:00 (1 cycle); Activation 95 °C 0:15; Denature 60 °C 0:30; Anneal\/Extend 72 °C 0:30 (read fluorescence during this step); 40 cycles. (Note: ABI7500 users: change final step timing to 72 °C 34 s.)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eABI 7500, Bio-Rad CFX96, SHENTEK-96S, Roche LightCycler 480 (and other real-time PCR instruments with appropriate dye settings)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThreshold settings (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eReference-5 threshold = 0.06; Target-5 threshold = 0.02; Manual Ct, Automatic baseline\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNTC \/ NCS acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eCt (Reference and Target) for NTC should be \u0026gt;35.00 or undetermined. Ct values for NCS should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReplicates\u003c\/td\u003e\r\n                        \u003ctd\u003eTriplicates recommended for standards, controls and samples\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalculation of rcAAV contamination rate\u003c\/td\u003e\r\n                        \u003ctd\u003eContamination rate = (Detection value of Target genes) ÷ (Detection value of Reference genes × dilution factor ÷ 2). Note: each rAAV contains two copies of Reference gene.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ercAAV-specific detection:\u003c\/strong\u003e Designed to detect replication-competent AAV contamination (rcAAV) in rAAV-5\/N serotypes by targeting the ITR sequence.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes a T\u0026amp;R DNA control and instructions for preparing a 6-point standard curve enabling absolute quantitation (copies\/µL).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Separate Reference and Target Primer\u0026amp;Probe MIX-5 provided to streamline assay setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (ABI 7500, CFX96, SHENTEK-96S, Roche 480) with guidance for ROX usage per instrument.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQuality control guidance:\u003c\/strong\u003e Includes recommended controls (NTC, NCS) and acceptance criteria (Ct thresholds) to support assay validity.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of rcAAV contamination in rAAV-5\/N production lots\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing of rAAV bulk and purified end-products\u003c\/li\u003e\r\n                    \u003cli\u003eTesting harvested cell-culture samples for rcAAV following infection\/production\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring during rAAV manufacturing and downstream purification\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eT\u0026amp;R DNA Control-5 (NNA028)\u003c\/span\u003e\u003cstrong\u003e-20 °C (lyophilized)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ercAAV qPCR Reaction Buffer (NNB009) 400 µL × 4 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTarget Primer\u0026amp;Probe MIX-5 (NNC050) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReference Primer\u0026amp;Probe MIX-5 (NNC051) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× ROX (NND007) 20 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eddH2O (NND010) 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 2 × 100 reactions | Method: qPCR (absolute quantitation \/ standard curve)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Benzonase nuclease treatment of rAAV stock or final product is recommended prior to nucleic acid extraction to remove unprotected nucleic acids and improve accuracy of direct qPCR.\u003c\/p\u003e\r\n                    \u003cp\u003e• Work area, pipettes and tubes should be decontaminated (UV 30 min, 75% ethanol) before setup to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge prior to use. Reconstitute lyophilized DNA control with precise volume and ensure complete dissolution (centrifuge and invert as instructed).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components under recommended conditions and check expiration date. Unused DDB should be stored at 2–8 °C; if cloudy, warm to 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five concentration points in the standard curve; perform triplicates for standards, controls and samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure sample dilutions place sample within the standard curve range (20–2 × 10^6 copies\/µL) for Reference gene. Target gene may be tested undiluted for high-titer samples; dilute Reference measurements as needed.\u003c\/p\u003e\r\n                    \u003cp\u003e• Set instrument-specific ROX usage according to instructions (e.g., ABI7500: add 0.04 µL ROX per reaction; other instruments may not require ROX).\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC Ct values should be \u0026gt;35 or undetermined; NCS Ct must be larger than mean Ct of lowest standard. Multiply measured concentration by sample dilution factors for final copies\/mL reporting.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403445.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403445.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the quantitation range of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe validated standard curve range is 20 to 2 × 10^6 copies\/µL (use ST1–ST6 standards as described).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store the kit and reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures; frozen components are stored at -20 °C. Check individual component labels and the user guide for storage and stability (kit stable for up to 24 months).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be included with each run?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a no-template control (NTC), a negative quality control (NCS), and a standard curve (minimum five concentrations recommended). Test samples and controls should be run in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How is rcAAV contamination rate calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eContamination rate = (Target gene copies) ÷ (Reference gene copies × dilution factor ÷ 2). Note that each rAAV contains two copies of the Reference gene.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions x 2","offer_id":45181756375221,"sku":"1403445","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rcAAV-5_N_Quantitation_Kit.jpg?v=1742362372"},{"product_id":"fungi-bacteria-dna-extraction-kit-microshentek®","title":"Fungi \u0026 Bacteria DNA Extraction Kit MicroSHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature \/ 2-8 °C \/ -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eMicroSHENTEK® Fungi \u0026amp; Bacteria DNA Extraction Kit is a magnetic-particle based nucleic acid extraction kit designed to isolate trace fungal and bacterial DNA from complex matrices (≤ 10^6 cells) including master cell banks, working cell banks, vaccines and cellular therapeutic products. The kit is intended to be used together with MicroSHENTEK® Fungi DNA Detection Kit and MicroSHENTEK® Bacteria DNA Detection Kit to provide an integrated workflow from sample preparation to qPCR detection. The kit is compatible with automated extraction on the rHCDpurify system and supports inclusion of an internal control for fungal extraction. Recommended system extraction performance is 50–100 CFU per tube, and the corresponding detection kit limit of detection is ≤35 CFU\/reaction.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat \/ Capacity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 extractions; Product No.: 1504633\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended extraction performance\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e50–100 CFU per tube\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCorresponding detection kit LOD\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e≤35 CFU per reaction (when used with MicroSHENTEK detection kits)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input matrix\u003c\/td\u003e\r\n                        \u003ctd\u003eMaster cell banks, working cell banks, vaccines, cellular therapeutic products and other complex matrices (≤10^6 cells)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDigestion conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 10 μL 5M NaCl, 10 μL Proteinase K and 100 μL Lysis buffer; incubate at 55 °C for 40–60 min (vortex and extend as needed up to complexity of matrix)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCell wall fragmentation (bead mill) recommended settings\u003c\/td\u003e\r\n                        \u003ctd\u003e5 m\/s for 40 s, 1 cycle\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCentrifugation\u003c\/td\u003e\r\n                        \u003ctd\u003e16200×g, 4 °C for 15–30 min (sample enrichment) and ≥16200×g for at least 2 min after fragmentation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAutomated extraction runtime\u003c\/td\u003e\r\n                        \u003ctd\u003erHCDpurify Micro-633 program, approximately 52 minutes per run\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum interval between extractions\u003c\/td\u003e\r\n                        \u003ctd\u003e30 minutes (to allow UV\/cleaning between runs)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check labels for expiration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eEluate volume requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eEnsure \u0026gt;40 μL bottom eluate for downstream assay\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Recommended extraction performance of 50–100 CFU\/tube and compatible with detection kit LOD ≤35 CFU\/reaction.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAutomation compatible:\u003c\/strong\u003e Validated for automated extraction using the rHCDpurify system (Micro-633 program, ~52 min).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Optimized for complex matrices including master\/working cell banks, vaccines and cell therapy products (≤10^6 cells).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eIntegrated workflow:\u003c\/strong\u003e Designed to work together with MicroSHENTEK® Fungi and Bacteria DNA Detection Kits for direct downstream qPCR.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal control support:\u003c\/strong\u003e Provides an option to add fungal internal control (IC) during sample preparation for extraction quality monitoring.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eTrace fungi and bacteria DNA extraction from master cell banks and working cell banks\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for microbial testing of vaccines and cellular therapeutic products\u003c\/li\u003e\r\n                    \u003cli\u003eExtraction from other complex biological matrices prior to qPCR-based microbial detection\u003c\/li\u003e\r\n                    \u003cli\u003eAutomated high-throughput extraction on rHCDpurify system (96-deep well plate workflows)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 2 bottles\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 2 bottles\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 15 mL × 2 bottles (add 20 mL anhydrous ethanol before use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 2 bottles\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 2 bottles\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSample treatment tube (NND005)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 50 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 500 μL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND033)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 750 μL × 2 tubes (equilibrate to room temp and vortex before use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePrecipitation solution I (NND003)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 25 μL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePrecipitation solution II (NND004)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 μL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 μL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Extractions | Method: Automated magnetic particle-based DNA extraction (compatible with rHCDpurify); intended for downstream qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only; read Material Safety Data Sheets (MSDS) and use appropriate PPE (eye protection, mask, gloves, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Separate laboratory areas for negative control\/reagent prep, sample processing and positive control to avoid cross-contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Disinfect work areas with nucleic acid scavenger and 75% ethanol; perform UV irradiation of benches (≥1 hour) and work areas (≥30 minutes) before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Before and after rHCDpurify runs perform UV sterilization for at least 15 minutes and wipe with 75% ethanol; minimum interval between extractions is 30 minutes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain ambient experimental temperature ≥22 °C and humidity ≤70% during procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Change gloves and protective garments regularly and use different supplies in different areas to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure magnetic particles are equilibrated and vortexed prior to use; if Lysis or Binding solutions appear cloudy, warm to 37 °C and mix until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• After extraction immediately transfer purified eluate to a new tube and ensure at least 40 μL is recovered for downstream qPCR; perform qPCR the same day if possible.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1504633.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1504633.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity of this extraction kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe recommended extraction performance is 50–100 CFU per tube; when used with the corresponding MicroSHENTEK detection kits the detection limit is ≤35 CFU per reaction.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many extractions are provided per kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit contains reagents for 50 extractions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended storage conditions?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eKit components are stored at room temperature, 2–8 °C, or -20 °C depending on the component; overall stability up to 24 months. Check individual component labels for specific storage instructions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this kit be used with an automated extractor?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify system and the Micro-633 program (approx. 52 minutes run). Follow plate layouts and program settings in the user guide.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45187300065461,"sku":"1504633","price":3070.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fungi_Bacteria_DNA_Extraction_Kit-MicroSHENTEK.jpg?v=1742274582"},{"product_id":"fungi-dna-detection-kit-mycoshentek™","title":"Fungi DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMicroSHENTEK® Fungi DNA Detection Kit is a real-time PCR (qPCR) based qualitative assay designed to detect fungal DNA contamination in cells, cell products, vaccines and related matrices. The kit is intended to be used together with the MicroSHENTEK® Fungi \u0026amp; Bacteria DNA Extraction Kit for sample preparation. The assay has been validated according to USP, EP, JP and Ch.P standards and demonstrates a Limit of Detection of not more than 35 CFU\/reaction. The assay covers approximately 92% of known fungal species (~5,000 species\/subspecies) and has been validated across a variety of matrices and tested against non-fungal species, production cell lines and engineered bacteria. The kit includes qPCR master mix components, primer\/probe mix, internal control (IC), positive control (PC) and DNA dilution buffer (DDB).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd\u003eQualitative detection of fungal DNA contamination in cells, cell products and vaccines (used with MicroSHENTEK® Fungi \u0026amp; Bacteria DNA Extraction Kit).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNot more than 35 CFU\/reaction\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCovers ~92% of known fungal species (approximately 5,000 species\/subspecies)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP, EP, JP and Ch.P (listed references: USP\u0026lt;71\u0026gt;, EP\u0026lt;2.6.21\u0026gt;, EP\u0026lt;2.6.7\u0026gt;, EP\u0026lt;2.6.16\u0026gt;, EP\u0026lt;2.6.1\u0026gt;, JP\u0026lt;4.06\u0026gt;, Ch.P\u0026lt;1101\u0026gt;, Ch.P\u0026lt;0234\u0026gt;)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL total per reaction (10 µL qPCR MIX + 20 µL sample\/controls)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eFun qPCR Reaction Buffer 8 µL; Fun Primer \u0026amp; Probe MIX 1.5 µL; Fun Internal Control (IC) 0.5 µL; total 10 µL qPCR MIX (UNG enzyme 0.1 U per reaction recommended)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eUNG treatment: 25 °C 10:00 (1 cycle); Activation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 55 °C 00:30; Extension: 72 °C 01:00; 45 cycles. Fluorescence read during Extension step.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls and interpretation thresholds (example)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eControl acceptance criteria: NTC FAM Ct ≥ 39.00 (or no amplification). PC and PCS FAM\/VIC Ct \u0026lt; 35.00 with effective 'S'-type amplification. Test sample positive when FAM Ct \u0026lt; 39.00 (at least one well) and VIC Ct \u0026lt;40.00 with effective 'S'-type amplification. See user guide tables for full decision matrix.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with SHENTEK-96S Real-time PCR System, Roche LightCycler 480 II, and other real-time PCR systems supporting probe assays (FAM and VIC channels).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction setup recommendation\u003c\/td\u003e\r\n                        \u003ctd\u003ePerform reactions with duplicate wells for each sample; include NTC, NCS, PC and PCS as controls; prepare qPCR MIX in a negative area, add samples\/controls in designated laboratory areas to avoid contamination.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh coverage:\u003c\/strong\u003e Detects approximately 92% of known fungal species (~5,000 species\/subspecies) based on validated primer\/probe design.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive detection:\u003c\/strong\u003e Limit of detection ≤35 CFU\/reaction when method acceptance criteria are met.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePharmacopoeia-validated:\u003c\/strong\u003e Assay performance validated according to USP, EP, JP and Ch.P guidance and methods.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete QC controls:\u003c\/strong\u003e Includes internal control (IC), positive control (PC) and guidance for NTC\/NCS\/PCS to ensure run validity and detect PCR inhibition.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument flexibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (example: SHENTEK-96S and LightCycler 480 II); customizable program settings provided.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eContamination control workflow:\u003c\/strong\u003e Recommended divided laboratory workflow (negative\/sample\/positive areas), UNG treatment and explicit plate-loading order to reduce contamination risk.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQualitative detection of fungal contamination in cell cultures\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing of cell-derived products and biologics (e.g., vaccines)\u003c\/li\u003e\r\n                    \u003cli\u003eSterility testing workflows per pharmacopeia guidance\u003c\/li\u003e\r\n                    \u003cli\u003eContamination monitoring in production cell lines and biological manufacturing\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eFun qPCR Reaction Buffer (Part No. NNB017) — 220 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eFun Primer \u0026amp; Probe MIX (Part No. NNC086) — 50 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eFun Internal Control (IC) (Part No. NNA038) — 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eFun Positive Control (PC) (Part No. NNA042) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for diagnostic or clinical use); follow MSDS and local safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent nucleic acid contamination: disinfect work area, irradiate surfaces, pipettes and tubes with UV for 60 minutes and use 75% ethanol and nucleic acid scavenger.\u003c\/p\u003e\r\n                    \u003cp\u003e• Divide laboratory into Negative, Sample and Positive areas and use a single sterile desktop per area; add NTC and NCS in negative area, samples in sample area, and PCS\/PC in positive area.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use RNase\/DNase-free low-retention tubes and filtered tips; use recommended plate\/strip types and sealing films.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include appropriate controls (NTC, NCS, PCS, PC) and run in duplicate; follow acceptance criteria in the user guide for Ct thresholds and result interpretation.\u003c\/p\u003e\r\n                    \u003cp\u003e• UNG enzyme is recommended (0.1 U per reaction) to control carryover contamination — verify enzyme amount and compatibility with your instrument.\u003c\/p\u003e\r\n                    \u003cp\u003e• If PCR inhibition or ambiguous results occur (invalid results), repeat extraction and testing or contact technical support.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1504631.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1504631.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's sensitivity (limit of detection)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been validated to a detection limit of not more than 35 CFU per reaction when run according to the instructions and acceptance criteria provided in the user guide.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments. The user guide explicitly lists SHENTEK-96S and Roche LightCycler 480 II as examples; other qPCR systems supporting probe assays (FAM and VIC channels) can be used with appropriate program setup.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How many reactions are included and what is the reaction volume?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit supplies reagents for 50 reactions. The recommended total reaction volume is 30 µL (10 µL qPCR MIX + 20 µL sample or control).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included in each run?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a No Template Control (NTC), Negative Control Sample (NCS), Positive Control Sample (PCS) and Positive Control (PC) as specified. Run samples in duplicate and follow the Ct acceptance criteria in the user guide to validate the run.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Assay performance validated per USP, EP, JP and Ch.P guidance.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45187307241653,"sku":"1504631","price":3399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fungi_DNA_Detection_Kit-MycoSHENTEK.jpg?v=1742275269"},{"product_id":"bacteria-dna-detection-kit-mycoshentek™","title":"Bacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eMicroSHENTEK® Bacteria DNA Detection Kit is used together with the MicroSHENTEK® Fungi \u0026amp; Bacteria DNA Extraction Kit to qualitatively determine bacterial contamination in cells, cell products, vaccines and related matrices. The assay uses real-time PCR (qPCR) and has been validated against USP, EP, JP and Ch.P requirements. The kit covers approximately 92% of known bacterial species (nearly 60,000 species\/subspecies sequences) and has a reported detection limit of not more than 35 CFU per reaction. Specificity and coverage have been validated across a variety of matrices, production cells and engineered fungi. For extraction procedures refer to MicroSHENTEK® Fungi \u0026amp; Bacteria DNA Extraction Kit User Guide (Product No. 1504633). Reagents provided are sufficient for 50 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNot more than 35 CFU\/reaction\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCoverage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eApproximately 92% of known bacterial species (~60,000 species\/subspecies sequences)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eValidated according to pharmacopeial guidance: USP, EP, JP and Ch.P (references include USP\u0026lt;71\u0026gt;, EP\u0026lt;2.6.21\u0026gt;, EP\u0026lt;2.6.7\u0026gt;, EP\u0026lt;2.6.16\u0026gt;, EP\u0026lt;2.6.1\u0026gt;, JP\u0026lt;4.06\u0026gt;, Ch.P\u0026lt;1101\u0026gt;, Ch.P\u0026lt;0234\u0026gt;)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd\u003eQualitative detection of bacterial DNA to assess bacterial contamination in cells, cell products, vaccines and related samples\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNumber of reactions per kit\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 μL total reaction volume (10 μL qPCR MIX + 20 μL sample\/control)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 55 °C 00:30; Extension: 72 °C 01:00; 45 cycles. Fluorescence read during extension.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControl acceptance thresholds (FAM channel)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct ≥ 39.00 or no amplification; NCS: Ct ≥ 39.00 or no amplification; PC: Ct \u0026lt; 35.00 with proper S-shaped amplification; PCS: Ct \u0026lt; 39.00 with S-shaped amplification\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTest sample interpretation (FAM channel)\u003c\/td\u003e\r\n                        \u003ctd\u003ePositive: Ct \u0026lt; 39.00 (at least one well) with S-shaped amplification. Negative: Ct ≥ 39.00 or absence of specific amplification in duplicate runs. If PCS Ct ≥ 39.00, experimental process needs checking or retest may be recommended.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with common real-time PCR systems (examples: SHENTEK-96S Real-time PCR System, Roche LightCycler 480 II)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReal-time qPCR detection:\u003c\/strong\u003e Uses probe-based real-time PCR for sensitive and specific detection of bacterial DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeial guidance:\u003c\/strong\u003e Assay performance validated according to USP, EP, JP and Ch.P standards for sterility \/ nucleic acid amplification methods.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh coverage:\u003c\/strong\u003e Designed to detect approximately 92% of known bacterial species (~60,000 sequences), increasing the likelihood of detecting diverse contaminants.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eLow detection limit:\u003c\/strong\u003e Reported detection limit of ≤35 CFU per reaction (LOD validated during method validation).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes positive control and procedural controls to support result interpretation and assay QC.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatibility and flexibility:\u003c\/strong\u003e Works with standard PCR plates\/strips and is compatible with multiple real-time PCR instruments; workflow supports separate negative\/sample\/positive areas to minimize contamination.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQualitative detection of bacterial contamination in cell cultures\u003c\/li\u003e\r\n                    \u003cli\u003eSterility testing for cell products and biologics (vaccines, therapeutics)\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control during production of biological products\u003c\/li\u003e\r\n                    \u003cli\u003eScreening of production cells and engineered fungi for bacterial DNA contamination\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications requiring sensitive detection of bacterial DNA in various matrices\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBac Positive Control (PC) (Part No. NNA047) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBac qPCR Reaction Buffer (Part No. NNB019) 220 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBac Primer \u0026amp; Probe MIX (Part No. NNC098) 50 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for human diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Work areas should be segregated into Negative, Sample and Positive areas; use dedicated equipment in each area to prevent contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes by UV irradiation (60 minutes) and 75% ethanol and use nucleic acid scavenger as needed.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare dilutions of positive control last and perform dilutions in the positive area to avoid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow specified plate loading order: add and seal NTC and NCS in negative area, add test samples in sample area, then add PCS and PC in positive area.\u003c\/p\u003e\r\n                    \u003cp\u003e• If detecting fungi and bacteria in the same run, add UNG enzyme step (25 °C for 10 minutes) and perform two-channel assay (FAM and VIC) as instructed.\u003c\/p\u003e\r\n                    \u003cp\u003e• If control criteria are not met (e.g., PCS Ct ≥39.00), re-check experiment and consider retesting.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1504632.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1504632.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit reports a detection limit of not more than 35 CFU per reaction (LOD as validated during method validation).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which real-time PCR instruments are supported?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems. Examples provided: SHENTEK-96S Real-time PCR System and Roche LightCycler 480 II. Other instruments may be used if configured to the specified reaction volume and cycling program.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C. Components are stable for up to 24 months; check the label expiration date.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNTC and NCS: Ct ≥ 39.00 (or no amplification). PC: Ct \u0026lt; 35.00 with effective S-shaped amplification. PCS: Ct \u0026lt; 39.00 with effective S-shaped amplification. If PCS Ct ≥ 39.00, the process should be rechecked.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Assay performance validated according to USP, EP, JP and Ch.P guidance (e.g., USP\u0026lt;71\u0026gt;, EP\u0026lt;2.6.21\u0026gt;, JP\u0026lt;4.06\u0026gt;, Ch.P references).\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45187308191925,"sku":"1504632","price":3399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fungi_Bacteria_DNA_Extraction_Kit.jpg?v=1742275516"},{"product_id":"bvdv-detection-kit-shentek®","title":"BVDV RT-qPCR Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® BVDV Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay format \/ reaction volume\u003c\/td\u003e\n\u003ctd\u003e20 µL total reaction volume (10 µL RT-qPCR MIX + 10 µL extracted sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT-qPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eBVDV Primer \u0026amp; Probe MIX 3.7 µL; 5×RT-qPCR Buffer 4 µL; RT-qPCR Enzyme MIX 1 µL; IPC MIX 1.3 µL; Total 10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 45 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during anneal\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eNo cross-amplification with bovine, porcine genomes, cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or expression bacteria (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls and acceptance criteria (Ct thresholds)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; NCS: FAM Ct ≥ 40 or no amplification; PCS: FAM Ct \u0026lt; 35 and amplification. Test sample interpretation uses FAM and VIC channels: Positive if FAM Ct \u0026lt; 40 (specific amplification); Presumptive positive if FAM ≥ 40 with VIC \u0026lt; 40 (indicates inhibitors); Negative if FAM ≥ 40 and VIC \u0026lt; 40 absent.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage and stability\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore components at –20 °C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and most standard real-time PCR systems supporting probe assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification observed with bovine\/porcine genomes, common cell lines or typical expression organisms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesigned for complex matrices:\u003c\/strong\u003e Validated for detection in cell cultures and bovine serum when used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Includes IPC MIX to monitor for PCR inhibition and sample\/extraction performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix and enzyme\/buffer mixes reduce setup variability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with multiple common real-time PCR instruments and standard probe detection settings (FAM for target, VIC for IPC).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BVDV target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BVDV nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing BVDV target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBVDV Primer \u0026amp; Probe MIX (NNC091)\u003c\/span\u003e\u003cstrong\u003e200 µL × 1 tube; –20 °C; protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX (NNC079)\u003c\/span\u003e\u003cstrong\u003e100 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (NNC066)\u003c\/span\u003e\u003cstrong\u003e150 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer (NNB018)\u003c\/span\u003e\u003cstrong\u003e300 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e1.2 mL × 1 tube; store nuclease-free (room temperature or –20 °C as per lab practice)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic human use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and certified RNase\/DNase-free reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect light-sensitive reagents (primer \u0026amp; probe mix) from light.\u003c\/p\u003e\n\u003cp\u003e• If VIC (IPC) signal indicates inhibition, repeat sample preparation or remove inhibitors and retest.\u003c\/p\u003e\n\u003cp\u003e• Check expiration dates on labels; store components at –20 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506731.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506731.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is 50 copies per reaction (LOD = 50 copies\/reaction).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: What samples can be tested with this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for testing cell culture samples (≤10^7 cells), bovine serum and other sample types after appropriate extraction with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit. For special sample matrices consult technical support.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended reaction and thermal cycling conditions?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eUse a 20 µL total reaction (10 µL RT-qPCR MIX + 10 µL extracted sample). Thermal program: Reverse transcription 50 °C 15:00; Activation 95 °C 00:30; 45 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence during annealing\/extension).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the internal control (VIC) is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf VIC signal is inhibited, repeat sample preparation or apply inhibitor removal steps during extraction and retest the sample.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45187313631413,"sku":"1506731","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BVDV_Detection_Kit.jpg?v=1742276076"},{"product_id":"anti-e-coli-protein-expression-strains-hcp-antibody-shentek®","title":"Anti-E.coli (Protein Expression Strains) HCP Antibody  SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term) (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003ePolyclonal sheep IgG antibody raised against E.coli host cell proteins (HCPs) from BL21 produced by physical disruption. Supplied as an affinity-purified, unconjugated liquid antibody in PBS without preservatives. Intended for research applications including HCP coverage evaluations, Western blot and ELISA. Supplied as 1 mg of antibody. Maintain at 2-8°C for short-term use; store below -65°C for long-term storage. Validity period 12 months.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eEscherichia coli (E. coli)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost\/Isotype\u003c\/td\u003e\r\n                        \u003ctd\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eConjugate\u003c\/td\u003e\r\n                        \u003ctd\u003eUnconjugate\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eE.coli HCPs (BL21) produced by physical disruption\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAffinity Purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage buffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP Coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8°C for short-term use; for long-term storage, store below -65°C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd\u003e12 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad HCP recognition:\u003c\/strong\u003e Polyclonal antibody generated against whole-cell E.coli BL21 HCP mixture to provide broad host cell protein coverage.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity purified:\u003c\/strong\u003e Affinity purification improves specificity and reduces background in immunoassays.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eApplications ready:\u003c\/strong\u003e Validated for HCP coverage evaluation workflows and compatible with Western blot and ELISA formats.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePreservative-free formulation:\u003c\/strong\u003e Supplied in PBS without preservatives for compatibility with downstream assays and conjugation.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluations\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot (WB)\u003c\/li\u003e\r\n                    \u003cli\u003eEnzyme-linked immunosorbent assay (ELISA)\u003c\/li\u003e\r\n                    \u003cli\u003eResearch use in process development and assay characterization involving E. coli-expressed proteins\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli (Protein Expression Strains) HCP Antibody\u003c\/span\u003e\u003cstrong\u003e1 mg; store 2-8 °C for short-term; store \u0026lt; -65 °C for long-term\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStorage buffer: PBS\u003c\/span\u003e\u003cstrong\u003eStore 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable (antibody reagent) | Method: ELISA, Western Blot, HCP coverage evaluation\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not intended for diagnostic purposes).\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8°C for short-term use; for long-term storage, store below -65°C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; aliquot as required.\u003c\/p\u003e\r\n                    \u003cp\u003e• Verify performance and optimal dilution in your assay system prior to critical experiments.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use beyond the stated validity period (12 months).\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301301-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301301-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store this antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMaintain at 2-8°C for short-term use. For long-term storage, store below -65°C. Avoid repeated freeze–thaw cycles by aliquoting upon receipt.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What applications is this antibody suitable for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis antibody is intended for HCP coverage evaluations and is compatible with Western blot and ELISA formats. Users should optimize assay conditions (dilution, blocking, incubation times) for their specific workflow.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is the antibody conjugated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is supplied unconjugated (native IgG). It can be conjugated by the user to labels (HRP, biotin, fluorophores) if required.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this product be used for diagnostic purposes?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is for research use only (RUO) and is not intended for diagnostic or therapeutic applications.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for diagnostic or therapeutic use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45187338764469,"sku":"1301301-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-E.coli_Protein_Expression_Strains_HCP_Antibody.jpg?v=1742277588"},{"product_id":"anti-e-coli-k-12-alkaline-lysis-hcp-antibody-shentek®","title":"Anti-E.coli (K-12 \u0026 Alkaline Lysis) HCP Antibody SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (short-term); \u0026lt; -65 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK™ Anti-E.coli (K-12 \u0026amp; Alkaline Lysis) HCP Antibody is an affinity-purified polyclonal sheep IgG raised against Escherichia coli host cell proteins (HCPs) derived from K-12 strains prepared by alkaline lysis. The reagent is supplied as a liquid, unconjugated antibody in PBS without preservatives and is intended for research applications including HCP coverage evaluations, Western blot and ELISA. Supplied at 1 mg per vial. Store at 2-8 °C for short-term use and below -65 °C for long-term storage. This product is for research use only (RUO) and is not for diagnostic purposes.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct No.\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1301302-Ab01\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSize\u003c\/td\u003e\r\n                        \u003ctd\u003e1 mg\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies Reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eEscherichia coli (E. coli), K-12 strains\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eHost \/ Isotype\u003c\/td\u003e\r\n                        \u003ctd\u003eSheep \/ IgG\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eClass\u003c\/td\u003e\r\n                        \u003ctd\u003ePolyclonal\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eType\u003c\/td\u003e\r\n                        \u003ctd\u003eAntibody, unconjugated\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eImmunogen\u003c\/td\u003e\r\n                        \u003ctd\u003eE. coli HCPs (K-12 strains) produced chemically by alkaline lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eForm\u003c\/td\u003e\r\n                        \u003ctd\u003eLiquid\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePurification\u003c\/td\u003e\r\n                        \u003ctd\u003eAffinity Purified\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage buffer\u003c\/td\u003e\r\n                        \u003ctd\u003ePBS\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eContains\u003c\/td\u003e\r\n                        \u003ctd\u003eNo preservatives\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eApplications\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eHCP Coverage evaluations, Western Blot, ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMaintain at 2-8 °C for short-term use. For long-term storage, store below -65 °C.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eValidity period\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e12 months (when stored as recommended)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAffinity-purified polyclonal antibody:\u003c\/strong\u003e Provides broad coverage of E. coli host cell proteins derived from K-12 strains.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eProduced against alkaline-lysis HCPs:\u003c\/strong\u003e Immunogen produced by chemical alkaline lysis to represent HCPs from K-12 strains.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eUnconjugated format:\u003c\/strong\u003e Suitable for use as primary antibody in ELISA, Western blot and HCP coverage evaluation workflows.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eNo preservatives:\u003c\/strong\u003e Formulated in PBS without preservatives to minimize interference in downstream assays.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eHCP coverage evaluations\u003c\/li\u003e\r\n                    \u003cli\u003eWestern Blot (WB)\u003c\/li\u003e\r\n                    \u003cli\u003eEnzyme-Linked Immunosorbent Assay (ELISA)\u003c\/li\u003e\r\n                    \u003cli\u003eResearch use in process development and HCP monitoring for E. coli-derived products\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli (K-12 \u0026amp; Alkaline Lysis) HCP Antibody, 1 mg\u003c\/span\u003e\u003cstrong\u003e2-8 °C (short-term); \u0026lt; -65 °C (long-term)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStorage buffer: PBS\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (antibody reagent) | Method: ELISA \/ Western Blot (antibody compatibility)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Not intended for diagnostic purposes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain at 2-8 °C for short-term use; for long-term storage store below -65 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not freeze-thaw repeatedly; aliquot to avoid multiple freeze-thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Contains no preservatives; handle under aseptic conditions where appropriate.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301302-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301302-Ab01.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended storage condition for this antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore at 2-8 °C for short-term use. For long-term storage, keep below -65 °C. Product is stable for 12 months under recommended storage.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What applications is this antibody validated for?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis antibody is intended for HCP coverage evaluations and is suitable for use in Western blot and ELISA-based assays.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is this product suitable for diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is provided for research use only (RUO) and is not intended for diagnostic purposes.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What is the immunogen used to generate this antibody?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe antibody was raised against E. coli host cell proteins (K-12 strains) produced chemically by alkaline lysis.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) - Not for diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"1 mg","offer_id":45188766859445,"sku":"1301302-Ab01","price":3914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Anti-E.coli_K-12_AlkalineLysis_HCPAntibody.jpg?v=1742279493"},{"product_id":"pi-3-detection-kit-shentek™","title":"PI-3 RT-qPCR Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    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space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThe SHENTEK® PI-3 Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eQualitative RT-qPCR (probe-based)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended samples\u003c\/td\u003e\n\u003ctd\u003eCell banks, virus seed banks, bovine serum (other matrices consult technical support)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volume\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e20 μL total per reaction (10 μL RT-qPCR MIX + 10 μL extracted sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT-qPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003ePI-3 Primer \u0026amp; Probe MIX 3.7 μL; 5×RT-qPCR Buffer 4 μL; RT-qPCR Enzyme MIX 1 μL; IPC MIX 1.3 μL; total 10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 00:30 (1 cycle); Denaturation: 95 °C 00:15 (45 cycles); Annealing\/extension: 60 °C 01:00 (45 cycles, fluorescence read during this step)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCt interpretation criteria (controls)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM Ct ≥ 40.00 or no amplification; PCS: FAM Ct \u0026lt; 35.00 and VIC Ct \u0026lt; 35.00 for duplicate runs; NCS: FAM Ct ≥ 40.00 and VIC Ct \u0026lt; 35.00 for duplicate runs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCt interpretation criteria (test samples)\u003c\/td\u003e\n\u003ctd\u003ePositive: FAM Ct \u0026lt; 40.00 (at least one well) with specific amplification and VIC Ct \u0026lt; 40.00 (duplicate runs); Presumptive positive with PCR inhibitors: FAM Ct ≥ 40.00 and VIC Ct \u0026lt; 40.00; Negative: FAM Ct ≥ 40.00 (no amplification) and VIC Ct \u0026lt; 40.00; Not conclusive if VIC inhibited (repeat extraction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility (instruments)\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96 and other real-time PCR systems supporting probe chemistry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check label expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack size \/ Reactions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity down to 50 copies per reaction (LOD).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification observed with bovine\/porcine genomes, common engineered cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or common production microbes (E. coli, Pichia pastoris).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated controls:\u003c\/strong\u003e Includes internal positive control (IPC) and recommended positive\/negative controls to monitor extraction and amplification performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized master mix:\u003c\/strong\u003e Ready-to-use primer\/probe mix and RT-qPCR enzyme\/buffer mixes for streamlined setup and consistent performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with standard workflows:\u003c\/strong\u003e Designed to be used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit and compatible with common real-time PCR instruments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuitable for complex matrices:\u003c\/strong\u003e Validated for detection in cell culture and bovine serum; extraction workflow recovers PI-3 RNA from complex samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of PI-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for PI-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing PI-3 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePI-3 Primer \u0026amp; Probe MIX (NNC092)\u003c\/span\u003e\u003cstrong\u003e200 μL × 1 tube; –20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX (NNC079)\u003c\/span\u003e\u003cstrong\u003e100 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (NNC066)\u003c\/span\u003e\u003cstrong\u003e150 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer (NNB018)\u003c\/span\u003e\u003cstrong\u003e300 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e1.2 mL × 1 tube; store at –20 °C or as recommended\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedures in a biosafety cabinet following BSL-2 guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free (RNase\/DNase-free) consumables and reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer\/probe mix from light.\u003c\/p\u003e\n\u003cp\u003e• Follow SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit user guide (Product No. 1506730) for sample preparation and extraction.\u003c\/p\u003e\n\u003cp\u003e• If VIC (IPC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat assay.\u003c\/p\u003e\n\u003cp\u003e• Do not use beyond expiration date printed on the kit labels.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506732.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506732.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity (LOD) of the SHENTEK PI-3 Detection Kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity (limit of detection) is 50 copies per reaction when used with the recommended extraction procedure.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported by the kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for detection of PI-3 in cell culture samples (≤10^7 cells) and bovine serum. For other sample matrices, consult technical support.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be run with each assay?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude a No Template Control (NTC), a Negative Control Sample (NCS), and a Positive Control Sample (PCS). Use duplicates for each control and test sample as recommended.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the internal positive control (VIC) is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf the VIC IPC signal is inhibited, repeat sample extraction with measures to remove inhibitors (e.g., additional purification) and retest the sample.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45188914905269,"sku":"1506732","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PI-3_Detection_Kit.jpg?v=1742280533"},{"product_id":"pi-3-positive-control-shentek®","title":"PI-3 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    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display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® PI-3 Positive Control is an armored RNA positive control intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and SHENTEK® PI-3 \/ Bovine Virus Detection Kits. It is designed to monitor the entire process of PI-3 RNA extraction and subsequent RT‑PCR detection, serving as a process\/control sample to validate extraction efficiency and RT‑PCR performance. The product is supplied as ready-to-use aliquots and is formulated to be non-infectious (armored RNA) while mimicking target viral RNA during extraction and amplification workflows.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eArmored RNA positive control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume per tube\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of tubes\u003c\/td\u003e\n\u003ctd\u003e3 tubes per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore below -65 °C; stable for up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess\/positive control for PI-3 RNA extraction and RT‑PCR detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility\u003c\/td\u003e\n\u003ctd\u003eCompatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit, SHENTEK® PI-3 Detection Kit, and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUse preparation\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReady for direct use without dilution; thaw and equilibrate before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSafety\u003c\/td\u003e\n\u003ctd\u003eArmored RNA (non-infectious surrogate); handle in biosafety cabinet and autoclave experimental waste\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull-process positive control:\u003c\/strong\u003e Monitors both RNA extraction and RT‑PCR detection steps to validate workflow integrity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA formulation:\u003c\/strong\u003e Non-infectious armored RNA that mimics native viral RNA behavior during extraction and amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Supplied at working concentration — no dilution required prior to use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term stability:\u003c\/strong\u003e Stable when stored below -65 °C for up to 12 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKit compatibility:\u003c\/strong\u003e Designed for seamless use with SHENTEK® extraction and detection kits for PI-3 and bovine virus panels.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-use aliquots:\u003c\/strong\u003e Provided as 100 μL tubes to minimize freeze–thaw and cross-contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eProcess control for PI-3 RNA extraction workflows\u003c\/li\u003e\n\u003cli\u003ePositive control for RT‑PCR assays targeting PI-3\u003c\/li\u003e\n\u003cli\u003eQuality control and validation of SHENTEK® extraction and detection kits\u003c\/li\u003e\n\u003cli\u003eOperator training and proficiency testing\u003c\/li\u003e\n\u003cli\u003eInter-run and lot-to-lot performance monitoring\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePI-3 Positive Control (armored RNA), 100 μL × 3 tubes\u003c\/span\u003e\u003cstrong\u003eStore below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (3 control tubes, 100 μL each) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation protocol described in the user guide of the SHENTEK® PI-3 Detection Kit and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3).\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Use only after equilibrating to room temperature; avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different production batches.\u003c\/p\u003e\n\u003cp\u003e• Do not use reagents beyond the expiration date shown on the label.\u003c\/p\u003e\n\u003cp\u003e• Although armored RNA is non-infectious, operate in a biosafety cabinet where possible; sterilize experimental waste by autoclaving and dispose of according to medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506738.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506738.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the SHENTEK® PI-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIt is intended as a process\/positive control to monitor PI-3 RNA extraction and RT‑PCR detection workflows when used with SHENTEK® extraction and detection kits.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the control be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the control below -65 °C. It is stable for up to 12 months; always check the expiration date on the label.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Do I need to dilute the PI-3 Positive Control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is supplied ready for direct use without dilution. Thaw, vortex, briefly spin, and equilibrate to room temperature before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the PI-3 Positive Control infectious and are special biosafety procedures required?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe control is an armored RNA surrogate and is non-infectious; however, it is recommended to handle it in a biosafety cabinet when possible and to autoclave\/dispose of experimental waste according to institutional regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45188968054965,"sku":"1506738","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PI-3_Positive_Control.jpg?v=1742282642"},{"product_id":"reo-3-detection-kit-shentek®","title":"REO-3 Analysis Kit  SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n 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#0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® REO-3 Analysis Kit is a qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Reovirus 3 (REO-3) contamination in cell banks, virus seed banks and bovine serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay sensitivity \/ Limit of detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification with bovine, porcine genomes, common production\/engineering cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volume\u003c\/td\u003e\n\u003ctd\u003e20 μL total reaction volume (10 μL RT-qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 00:30 (1 cycle); Denaturation: 95 °C 00:15; Annealing\/extension: 60 °C 01:00; 45 cycles (fluorescence read during annealing\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls and interpretation thresholds\u003c\/td\u003e\n\u003ctd\u003eControls: NTC, NCS, PCS, Internal Positive Control (IPC). Typical Ct acceptance criteria: FAM (target): Positive if Ct \u0026lt; 40.00 (at least one well) or Ct \u0026lt; 40 with duplicate amplification; VIC (IPC): Ct \u0026lt; 35.00 indicates valid IPC amplification. NTC\/NCS: no specific amplification (Ct ≥ 40 or absent) required. See user guide tables for full interpretation rules.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore components at -20 °C (recommended). Kit components stable for up to 24 months; items with recommended storage at -20 °C should be maintained within ±10 °C (–30 °C to –10 °C).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and other real-time PCR systems supporting probe assays and user-defined cycling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity down to 50 copies per reaction (LOD).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification with common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or bacterial\/yeast genomes (E. coli, Pichia pastoris), and with bovine\/porcine genomes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComplete RT-qPCR solution:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, RT-qPCR enzyme mix, buffer and internal positive control mix for end-to-end detection when combined with recommended extraction kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with multiple instruments:\u003c\/strong\u003e Validated on several common real-time PCR platforms and supports standard probe-based RT-qPCR setup.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesigned for complex matrices:\u003c\/strong\u003e Optimized to detect REO-3 RNA from cells and bovine serum when used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal control included:\u003c\/strong\u003e IPC MIX provided to monitor extraction and PCR inhibition.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of REO-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for REO-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing REO-3 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eREO-3 Primer \u0026amp; Probe MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (200 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C (100 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (Internal Positive Control MIX)\u003c\/span\u003e\u003cstrong\u003e-20 °C (150 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O\u003c\/span\u003e\u003cstrong\u003eRoom temperature or 2-8 °C as supplied (1.2 mL × 1 tube) — follow label instructions\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eKit format \/ quantity\u003c\/span\u003e\u003cstrong\u003eReagents for 50 reactions\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction in a biosafety cabinet and follow BSL-2 safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and certified RNase\/DNase-free reagents and tips to avoid contamination.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit completely at 2–8 °C or on ice, vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer \u0026amp; probe mixes from light.\u003c\/p\u003e\n\u003cp\u003e• Store components at -20 °C; short-term storage below -30 °C is acceptable if supported by stability data.\u003c\/p\u003e\n\u003cp\u003e• Check expiration dates on labels prior to use.\u003c\/p\u003e\n\u003cp\u003e• If IPC (VIC) signal indicates inhibition, repeat sample preparation or remove inhibitors and repeat assay.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506733.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506733.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's limit of detection (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eValidated sample types include cell culture (up to 1×10^7 cells per 100–200 μL) and bovine serum. For other matrices consult technical support and use the recommended SHENTEK® extraction kit.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls are required and how are results interpreted?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eRun NTC (No Template Control), NCS (Negative Control Sample), PCS (Positive Control Sample) and IPC. Typical acceptance thresholds: FAM target Ct \u0026lt; 40 indicates positive amplification; IPC (VIC) Ct \u0026lt; 35 indicates successful internal control amplification. Follow the user guide control tables for detailed interpretation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eCompatible with common real-time PCR systems including SHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and other instruments that support probe-based RT-qPCR.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45188988895413,"sku":"1506733","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/REO-3_Detection_Kit.jpg?v=1742283552"},{"product_id":"reo-3-positive-control-shentek®","title":"REO-3 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 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display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® REO-3 Positive Control is an armored RNA positive control intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and Detection Kits. It is supplied to monitor the entire process of REO-3 RNA extraction and RT-PCR detection assays. The product is provided ready for direct use (no dilution required) and supplied as 100 μL × 3 tubes. The control supports assay process monitoring, method validation and routine quality control. The product is for research use only and not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd\u003eArmored RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume per tube\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eBelow -65 °C; stable for up to 12 months (check expiration label)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003ePositive process control for REO-3 RNA extraction and RT-PCR detection assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUsage\u003c\/td\u003e\n\u003ctd\u003eReady for direct use without dilution; follow Positive Control Sample (PCS) preparation protocol in the corresponding SHENTEK detection kit user guide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull-process monitoring:\u003c\/strong\u003e Designed to monitor the entire workflow from RNA extraction through RT-PCR detection for REO-3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA format:\u003c\/strong\u003e Provides increased stability and safety compared with naked RNA; mimics viral RNA during extraction and detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Supplied ready for direct use without the need for dilution when prepared according to kit instructions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit, SHENTEK® REO-3 Detection Kit, and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eProcess control for REO-3 RNA extraction workflows\u003c\/li\u003e\n\u003cli\u003ePositive control for RT-PCR assay validation and routine QC\u003c\/li\u003e\n\u003cli\u003eAssay performance troubleshooting and operator training\u003c\/li\u003e\n\u003cli\u003eCompatibility testing with SHENTEK extraction and detection reagents\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eREO-3 Positive Control\u003c\/span\u003e\u003cstrong\u003e100 μL × 3 tubes; store below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (3 tubes of 100 μL each; used as positive control material) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Intended for research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex thoroughly and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation protocol in the SHENTEK® REO-3 Detection Kit or SHENTEK® Bovine Virus Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution when prepared per instructions.\u003c\/p\u003e\n\u003cp\u003e• Equilibrate to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Although armored RNA is safer than naked RNA, it is recommended to handle the product in a biosafety cabinet.\u003c\/p\u003e\n\u003cp\u003e• Experimental waste should be sterilized by autoclave and disposed of in accordance with medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506737.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506737.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the SHENTEK® REO-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the product below -65 °C. Under appropriate storage it is stable for up to 12 months; always check the expiration date on the label.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Do I need to dilute the positive control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is provided ready for direct use. Follow the PCS preparation protocol in the appropriate SHENTEK detection kit user guide.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the recommended thawing procedure?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThaw one tube completely at 2–8 °C or on ice, vortex thoroughly and briefly centrifuge prior to use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this product be used for clinical diagnostics?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for research use only (RUO) and is not for clinical diagnostic or therapeutic use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg fill=\"currentColor\" viewbox=\"0 0 20 20\" class=\"shentek-notice-icon\"\u003e\n                \u003cpath clip-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" fill-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45188999217333,"sku":"1506737","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/REO-3_Positive_Control.jpg?v=1742283721"},{"product_id":"bav-3-detection-kit-shentek®","title":"BAV-3 Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 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0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® BAV-3 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eQualitative probe-based real-time PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction format \/ volume\u003c\/td\u003e\n\u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL sample or control = 30 µL total\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eBAV-3 Primer \u0026amp; Probe MIX 2.8 µL; qPCR Reaction Buffer 15.9 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (45 cycles); Annealing\/extension 60 °C 01:00 (read fluorescence during this step)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and similar real-time PCR instruments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample input (recommended)\u003c\/td\u003e\n\u003ctd\u003eCell culture or bovine serum: 100–200 µL (≤10^7 cells). Follow Virus DNA \u0026amp; RNA Extraction Kit protocol for extraction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControl acceptance criteria (general)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35 indicates IPC amplification. PCS: Ct \u0026lt; 35 (FAM and VIC). See manual tables for full decision matrix.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification observed with bovine, porcine genomes; common production cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit capacity \/ reactions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\n\u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see storage information).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity down to 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification with tested bovine\/porcine genomes, common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated internal control:\u003c\/strong\u003e Includes IPC MIX to monitor extraction and PCR inhibition (VIC channel).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized for complex matrices:\u003c\/strong\u003e Validated for detection of BAV-3 DNA from cell culture and bovine serum when used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlatform flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR instruments (SHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96, etc.).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BAV-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BAV-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing BAV-3 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBAV-3 Primer \u0026amp; Probe MIX (NNC094)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (150 µL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 µL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (NNC066)\u003c\/span\u003e\u003cstrong\u003e-20 °C (150 µL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction steps in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables; avoid cross-contamination between samples, controls and reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components completely at 2–8 °C or on ice before use; vortex and briefly centrifuge.\u003c\/p\u003e\n\u003cp\u003e• Protect primer \u0026amp; probe mix from light.\u003c\/p\u003e\n\u003cp\u003e• If VIC (IPC) indicates inhibition, repeat sample preparation or remove inhibitors and repeat assay.\u003c\/p\u003e\n\u003cp\u003e• Follow instrument-specific setup and thresholding as described in the manual; validate control acceptance criteria during method validation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506734.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506734.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is 50 copies per reaction (LOD = 50 copies\/reaction).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 50 reactions.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What sample volume is recommended for cell culture or serum?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eCollect 100–200 µL of cell culture (≤10^7 cells) or bovine serum per sample and follow the Virus DNA \u0026amp; RNA Extraction Kit protocol for nucleic acid extraction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit components be stored?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at −20 °C (stable for up to 24 months); protect primer\/probe mix from light. Thaw at 2–8 °C or on ice before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189003313333,"sku":"1506734","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BAV-3_Detection_Kit.jpg?v=1742283990"},{"product_id":"bav-3-positive-control-shentek®","title":"BAV-3 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 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display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: 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.shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003ePCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® BAV-3 Positive Control is a ready-to-use positive control intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and Detection Kits. It is designed to monitor the entire process of BAV-3 DNA extraction and PCR detection assays. The product is supplied as an inactivated replication-defective pseudovirus, providing a safe surrogate for process control. Supplied as pre-aliquoted tubes, the control requires thawing, vortexing and brief centrifugation prior to use and can be used directly without dilution. This product is intended for research use only (RUO) and is not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eForm\u003c\/td\u003e\n\u003ctd\u003eInactivated replication-defective pseudovirus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess positive control for BAV-3 DNA extraction and PCR detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume per tube\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of tubes\u003c\/td\u003e\n\u003ctd\u003e3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage temperature\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eBelow -65 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStability \/ Shelf life\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStable for up to 12 months at recommended storage conditions; check expiration date on label\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePreparation\u003c\/td\u003e\n\u003ctd\u003eThaw completely at 2–8 °C or on ice, vortex thoroughly and centrifuge briefly before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUse condition\u003c\/td\u003e\n\u003ctd\u003eReady for direct use without dilution; equilibrate to room temperature before use; avoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility\u003c\/td\u003e\n\u003ctd\u003eDesigned for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit, SHENTEK® BAV-3 Detection Kit, and SHENTEK® Bovine Virus Detection Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiosafety\u003c\/td\u003e\n\u003ctd\u003eInactivated and replication-defective but recommended to handle in a biosafety cabinet; dispose of experimental waste by autoclaving per medical waste regulations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMixing batches\u003c\/td\u003e\n\u003ctd\u003eDo not mix reagents or controls from different batches\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProcess monitoring:\u003c\/strong\u003e Monitors the entire workflow from BAV-3 DNA extraction through PCR detection to validate assay performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Pre-aliquoted tubes supplied ready for direct use without dilution after thawing and brief mixing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe surrogate:\u003c\/strong\u003e Inactivated replication-defective pseudovirus provides a safe positive control for laboratory use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with SHENTEK® extraction and detection kits for streamlined workflow integration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQuality control for nucleic acid extraction (BAV-3 DNA)\u003c\/li\u003e\n\u003cli\u003ePositive control for PCR detection assays targeting BAV-3\u003c\/li\u003e\n\u003cli\u003eAssay validation and troubleshooting\u003c\/li\u003e\n\u003cli\u003eOperator training and proficiency testing\u003c\/li\u003e\n\u003cli\u003eInstrument and workflow verification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBAV-3 Positive Control\u003c\/span\u003e\u003cstrong\u003e100 μL × 3 tubes; store below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 3 tubes (100 μL each) — positive control, not measured in assay reaction count | Method: PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex thoroughly and centrifuge briefly.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation steps in the SHENTEK® BAV-3 Detection Kit or SHENTEK® Bovine Virus Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• Product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Equilibrate to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents or controls from different batches.\u003c\/p\u003e\n\u003cp\u003e• Although inactivated and replication-defective, operate in a biosafety cabinet; sterilize experimental waste via autoclaving and dispose per medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506739.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506739.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the SHENTEK® BAV-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the product below -65 °C. Under recommended conditions it is stable for up to 12 months. Always check the expiration date on the label before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare the positive control for use in my assay?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThaw one tube completely at 2–8 °C or on ice, vortex thoroughly and centrifuge briefly. The product is ready for direct use without dilution. Follow any additional preparation steps described in the SHENTEK® detection kit user guide.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this product be used for clinical diagnostics?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for research use only (RUO) and is not for clinical diagnosis or treatment.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the control safe to handle on the bench?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe control is an inactivated replication-defective pseudovirus and considered safe, but handling in a biosafety cabinet is recommended and experimental waste should be autoclaved and disposed of following medical waste regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189006721205,"sku":"1506739","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BAV-3_Positive_Control.jpg?v=1742284343"},{"product_id":"bpv-1-detection-kit-shentek®","title":"BPV-1 Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n  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.shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® BPV-1 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Bovine Parvovirus Type 1 (BPV-1) in cell banks, virus seed banks and bovine serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eNo cross-amplification detected with bovine and porcine genomes, common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or expression bacteria\/yeast (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample types\u003c\/td\u003e\n\u003ctd\u003eCell culture (≤10^7 cells), bovine serum, and other matrices after appropriate extraction; supports complex sample matrices after extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal reactions \/ Kit size\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction setup\u003c\/td\u003e\n\u003ctd\u003e20 µL qPCR MIX + 10 µL template (extracted sample \/ control \/ DDB); final reaction volume 30 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eBPV-1 Primer\u0026amp;Probe MIX 2.8 µL; qPCR Reaction Buffer 15.9 µL; IPC MIX 1.3 µL; Total 20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (45 cycles); Annealing\/Extension: 60 °C 01:00 (instrument reads fluorescence during this step)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls and interpretation thresholds (FAM \/ VIC)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct ≥ 40.00 or no amplification (FAM) \/ Ct \u0026lt; 35.00 for VIC indicates valid no-template control; NCS: Ct ≥ 40.00 or no amplification (FAM) \/ Ct \u0026lt; 35.00 (VIC); PCS: Ct \u0026lt; 35.00 (FAM and VIC); Test sample interpretation: Positive if FAM Ct \u0026lt; 40.00 (at least one well) with specific amplification and VIC Ct \u0026lt; 40.00 for duplicate runs; Presumptive positive if FAM Ct ≥ 40.00 or absent and VIC Ct \u0026lt; 40.00 indicating possible inhibitors, etc. See user guide tables for complete criteria.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96 and other standard real-time PCR systems supporting probe assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage stability \/ Shelf life\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions up to 24 months (see labels)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExtraction compatibility\u003c\/td\u003e\n\u003ctd\u003eValidated for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction enables detection of low-level BPV-1 contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification with bovine\/porcine genomes, common mammalian cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or common expression hosts (E. coli, Pichia pastoris).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComplete qPCR solution:\u003c\/strong\u003e Includes primer\/probe mix, qPCR reaction buffer and an internal positive control (IPC) to monitor inhibition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesigned for complex sample matrices:\u003c\/strong\u003e Works with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit to recover and detect viral DNA from cells and serum.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Compatible with major real-time PCR platforms (SHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BPV-1 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BPV-1 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing BPV-1 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBPV-1 Primer\u0026amp;Probe MIX (Part No. NNC095) 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (Part No. NNC066) 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedures in a biosafety cabinet and follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and reagents certified as RNase\/DNase-free.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer\/probe mix from light.\u003c\/p\u003e\n\u003cp\u003e• If VIC (internal control) signal indicates inhibition, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506735.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506735.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity reaches 50 copies per reaction (LOD = 50 copies\/reaction).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for cell culture samples (≤10^7 cells) and bovine serum. Other sample types may be processed after appropriate extraction; consult technical support for special matrices.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be included in each run?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude at minimum: No Template Control (NTC), Negative Control Sample (NCS), and Positive Control Sample (PCS). The guide recommends running samples in duplicate.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the internal control (VIC) is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf VIC signal is inhibited, repeat sample preparation or perform additional inhibitor removal steps, then re-run the assay. Persistent inhibition may require re-extraction or dilution of the nucleic acid.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189009834165,"sku":"1506735","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BPV-1_Detection_Kit.jpg?v=1742284559"},{"product_id":"bpv-1-positive-control-shentek®","title":"BPV-1 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    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italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003ePCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® BPV-1 Positive Control is intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and Detection Kits and serves as a positive control to monitor the entire process of BPV-1 DNA extraction and PCR detection assay. Supplied as an inactivated, replication-defective pseudovirus (100 μL × 3 tubes), the product is ready for direct use without dilution. It is intended for research use only and should be handled following appropriate biosafety procedures; experimental waste should be autoclaved and disposed of according to medical waste regulations.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eForm\u003c\/td\u003e\n\u003ctd\u003eInactivated replication-defective pseudovirus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd\u003eStore ≤ -65 °C; stable for up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003ePositive control for BPV-1 DNA extraction and PCR detection assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReady-to-use\u003c\/td\u003e\n\u003ctd\u003eProduct is provided ready for direct use without dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSafety\u003c\/td\u003e\n\u003ctd\u003eInactivated and replication-defective; recommended handling in a biosafety cabinet\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility\u003c\/td\u003e\n\u003ctd\u003eFor use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit and SHENTEK® BPV-1 Detection Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonitors entire workflow:\u003c\/strong\u003e Functions as a full-process positive control for extraction and PCR detection of BPV-1 DNA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Supplied ready for direct use without dilution to simplify QC procedures.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe pseudovirus format:\u003c\/strong\u003e Provided as an inactivated, replication-defective pseudovirus to reduce biosafety risk while mimicking real sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKit compatibility:\u003c\/strong\u003e Validated for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit and SHENTEK® BPV-1 Detection Kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eProcess control for BPV-1 DNA extraction and PCR detection assays\u003c\/li\u003e\n\u003cli\u003eInternal positive control for assay validation and quality control\u003c\/li\u003e\n\u003cli\u003eOperator training and proficiency testing\u003c\/li\u003e\n\u003cli\u003eMethod development and troubleshooting\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBPV-1 Positive Control (100 μL × 3 tubes)\u003c\/span\u003e\u003cstrong\u003e≤ -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A | Method: PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Intended for research use only; not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex thoroughly and centrifuge briefly.\u003c\/p\u003e\n\u003cp\u003e• Follow the preparation process for the Positive Control Sample (PCS) as outlined in the SHENTEK® BPV-1 Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Equilibrate to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Product is an inactivated replication-defective pseudovirus; recommended handling in a biosafety cabinet.\u003c\/p\u003e\n\u003cp\u003e• Experimental waste should be sterilized by autoclaving and disposed of according to medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506740.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506740.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended storage temperature for the BPV-1 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore at ≤ -65 °C; stable for up to 12 months. Avoid repeated freeze–thaw cycles.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Do I need to dilute the positive control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is supplied ready for direct use without dilution. Follow the SHENTEK® BPV-1 Detection Kit user guide for PCS preparation details.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this product be used for clinical diagnosis?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is for Research Use Only (RUO) and is not intended for clinical diagnostic or therapeutic use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the material infectious and what biosafety measures are required?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe product is an inactivated, replication-defective pseudovirus and is considered safe for laboratory use; however, it should be handled in a biosafety cabinet and waste should be autoclaved and disposed of according to regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189020090549,"sku":"1506740","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BPV-1_Positive_Control.jpg?v=1742284873"},{"product_id":"bovine-virus-detection-multiplex-kit-bvdv-reo-3-pi-3-bpv-3-bav-3","title":"Bovine Virus Analysis Multiplex Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n   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8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Bovine Virus Analysis Multiplex Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3) is a multiplex real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the simultaneous molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and multiplexed analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended reactions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 Reactions (kit provides sufficient reagents for 50 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volumes\u003c\/td\u003e\n\u003ctd\u003eRT-qPCR: 20 μL total (10 μL RT-qPCR MIX + 10 μL sample). qPCR (BPV-3\/BAV-3): 30 μL total (20 μL qPCR MIX + 10 μL sample).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling (RT-qPCR for BVDV\/REO-3\/PI-3)\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 40 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during annealing\/extension).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling (qPCR for BPV-3\/BAV-3)\u003c\/td\u003e\n\u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during annealing\/extension).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReporter dyes \/ Targets\u003c\/td\u003e\n\u003ctd\u003eREO-3: FAM; PI-3: HEX; BVDV: CY5; BPV-3: FAM; BAV-3: CY5; Internal Control (IC): ROX.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — controls\u003c\/td\u003e\n\u003ctd\u003eNTC: Undetected (no specific amplification); NCS: Undetected; PCS: Ct \u0026lt; 35.00 with specific amplification; ROX (IC) for controls: Ct \u0026lt; 35.00.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — test samples\u003c\/td\u003e\n\u003ctd\u003ePositive call: specific amplification with Ct \u0026lt; 40.00 in the relevant fluorescence channel. Sample inhibition check: compare sample ROX Ct to NCS ROX Ct; difference \u0026gt; 2 indicates possible inhibition. If Ctsample - CtNCS \u0026lt; 2 then negative; ≥ 2 not conclusive (PCR inhibitors possible).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S Real-time PCR System, LightCycler 480Ⅱ, ABI 7500 (other real-time PCR instruments may be used with appropriate setup).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls included \/ recommended\u003c\/td\u003e\n\u003ctd\u003eInternal Control (VIR IC) provided in kit; Positive Controls for individual targets sold separately (Product Nos. 1506736, 1506737, 1506738, 1506746, 1506739).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMultiplex detection:\u003c\/strong\u003e Simultaneous detection of three RNA viruses (BVDV\/REO-3\/PI-3) or two DNA viruses (BPV-3\/BAV-3) in a single well using multiplex real-time PCR.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal control provided:\u003c\/strong\u003e VIR Internal Control (IC) included to monitor extraction and amplification performance and identify inhibition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with standard instruments:\u003c\/strong\u003e Validated on SHENTEK-96S, LightCycler 480Ⅱ and ABI 7500; adaptable to other real-time PCR platforms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized workflow for complex matrices:\u003c\/strong\u003e Designed to work with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for efficient recovery from serum and cell matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing nucleic acids target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×Multiplex RT-PCR Buffer (NNB020), 200 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex RT-PCR Enzyme MIX (NNC102), 100 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex qPCR Reaction Buffer (NNB021), 850 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (NNA057), 600 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008), 1.2 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months) or as label indicates\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBV RNA Primer \u0026amp; Probe MIX (NNC103), 170 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months); protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBV DNA Primer \u0026amp; Probe MIX (NNC104), 120 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months); protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex IPC MIX (NNC105), 170 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: Multiplex RT-qPCR \/ qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\n\u003cp\u003e• Handle positive controls and extraction procedures in a biological safety cabinet following BSL-2 guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and reagents certified RNase\/DNase-free.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components completely at 2–8 °C or on ice, vortex thoroughly and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect probe-containing reagents from light.\u003c\/p\u003e\n\u003cp\u003e• Check control acceptance criteria (NTC\/NCS\/PCS\/IC Ct thresholds) for run validity.\u003c\/p\u003e\n\u003cp\u003e• If ROX (IC) Ct(sample) - ROX Ct(NCS) \u0026gt; 2, investigate potential PCR inhibition and repeat extraction\/dilution as needed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506741.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506741.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for bovine serum and cell culture matrices (cell banks). Use SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for nucleic acid extraction prior to detection.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has a reported limit of detection of 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be included in each run?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), and Positive Control Sample (PCS). An internal control (VIR IC) is provided to monitor extraction and amplification; add IC during extraction or to the reaction mix if not already added.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are positive\/negative results determined?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eA positive result is a well showing specific amplification with Ct \u0026lt; 40. For controls, PCS and ROX (IC) should have Ct \u0026lt; 35. Compare ROX Ct of sample to NCS to assess inhibition (difference \u0026gt; 2 suggests inhibition).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189025562805,"sku":"1506741","price":3399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Bovine_Virus_Detection_Multiplex_Kit_BVDV_REO-3_PI-3_BPV-3_BAV-3.jpg?v=1742285396"},{"product_id":"bpv-3-positive-control-kit-shentek®","title":"BPV-3 Positive Control Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003ePCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003e \u003c\/p\u003e\n\u003cp\u003eSHENTEK® BPV-3 Positive Control is an inactivated, replication-defective pseudovirus provided as a positive control to monitor the entire workflow of BPV-3 DNA extraction and PCR detection assays. It is intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kits and SHENTEK® Bovine Virus Detection Kits (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3). The product is supplied ready-to-use (no dilution required) and is formulated for research use only.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct No.\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e1506746\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eInactivated replication-defective pseudovirus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eQuantity\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore below -65 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStability\u003c\/td\u003e\n\u003ctd\u003eUp to 12 months (check label for expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003ePositive control for BPV-3 DNA extraction and PCR detection assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003eNot specified in document\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of detection (LOD) \/ Linear range \/ Accuracy\u003c\/td\u003e\n\u003ctd\u003eNot specified in document\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull-process control:\u003c\/strong\u003e Designed to monitor the entire process from BPV-3 DNA extraction to PCR detection, enabling workflow verification and quality control.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Product is provided ready for direct use without the need for dilution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe pseudovirus format:\u003c\/strong\u003e Inactivated, replication-defective pseudovirus formulation reduces biosafety risk while providing a biologically relevant control.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term storage stability:\u003c\/strong\u003e Stable when stored below -65 °C for up to 12 months (check expiration date).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit and SHENTEK® Bovine Virus Detection Kit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003ePositive control for BPV-3 DNA extraction procedures\u003c\/li\u003e\n\u003cli\u003ePositive control for PCR detection assays targeting BPV-3\u003c\/li\u003e\n\u003cli\u003eAssay validation and performance monitoring in research laboratories\u003c\/li\u003e\n\u003cli\u003eQuality control for kit lot verification and operator training\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBPV-3 Positive Control\u003c\/span\u003e\u003cstrong\u003e100 μL × 3 tubes; store below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable | Method: PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• This product is intended for research use only and not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex thoroughly and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation process as outlined in the SHENTEK® Bovine Virus Detection Kit user guide (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3).\u003c\/p\u003e\n\u003cp\u003e• Product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Equilibrate to room temperature before use; avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Although inactivated and replication-defective, operate in a biosafety cabinet when possible; sterilize experimental waste via autoclaving and dispose according to medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506746.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506746.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the SHENTEK® BPV-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIt is intended as a positive control to monitor BPV-3 DNA extraction and PCR detection assays, and to verify the performance of SHENTEK® Virus DNA \u0026amp; RNA Extraction Kits and SHENTEK® Bovine Virus Detection Kits.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the BPV-3 Positive Control be stored?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the product below -65 °C. It is stable for up to 12 months—check the expiration date on the label.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is any dilution required before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is provided ready for direct use without dilution. Thaw and mix as instructed before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is this product safe to handle on the bench?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe product is an inactivated replication-defective pseudovirus and is considered safe; however, it is recommended to handle it in a biosafety cabinet and to autoclave and properly dispose of experimental waste.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189042864309,"sku":"1506746","price":3399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BPV-3_Positive_Control_Kit.jpg?v=1742285847"},{"product_id":"mvm-detection-kit-shentek®","title":"MVM Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® MVM Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of MVM target sequences (covering strains i, p, m, and c) in industrial cell lines and related bioprocessing matrices (examples: CHO, BHK, NS0). Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), the assay includes an MVM-specific primer\/probe mix, an internal process control (IPC), and a positive control to monitor nucleic acid recovery and amplification. Combined with SHENTEK extraction, the kit enables the efficient recovery and highly sensitive analysis of target DNA from complex matrices such as cell suspensions and in vitro cell culture supplements. The assay demonstrates high specificity, with no cross-amplification observed against tested cell lines and non-target genomes, achieving a limit of testing (LoD) of 50 copies per reaction. The kit is supplied for 50 reactions.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Minute Virus of Mice (MVM) strains i, p, m and c in mouse-derived cell lines and serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnalytical sensitivity (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification observed with tested engineered cell lines (CHO, VERO, 293T, MDCK, NS0, Sf9) or engineered bacteria genomes (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volume\u003c\/td\u003e\n\u003ctd\u003e30 µL total reaction volume (20 µL qPCR MIX + 10 µL purified sample \/ control \/ NTC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eqPCR Master MIX 15.9 µL; MVM Primer \u0026amp; Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (add 0.5 µL VIR IC to mix if not added during extraction); total qPCR MIX = 20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation: 95 °C 10:00; 40 cycles of: Denaturation 95 °C 00:15, Annealing\/Extension 60 °C 01:00 (fluorescence read during annealing\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls provided \/ required\u003c\/td\u003e\n\u003ctd\u003eVIR Internal Control (IC) included; Positive Control (MVM Positive Control) is referenced (Product No. 1506747) and recommended; Negative control (DDB or 1×PBS) and NTC required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — control wells\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM undetected; VIC: Ct \u0026lt; 35 for duplicate runs. NCS: FAM undetected; VIC: Ct \u0026lt; 35. PCS: FAM Ct \u0026lt; 35 and VIC Ct \u0026lt; 35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — test samples\u003c\/td\u003e\n\u003ctd\u003ePositive: FAM Ct \u0026lt; 40 (at least one well) with specific amplification and VIC Ct \u0026lt; 35 in duplicates. Negative: FAM undetected in duplicates and Ctsample - CtNCS \u0026lt; 2. Not conclusive \/ possible inhibition: Ctsample - CtNCS ≥ 2 (compare VIC Ct of sample to NCS); retest or remove inhibitors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments (examples)\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500 and most real-time PCR systems supporting probe assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n\u003ctd\u003eKit components stored under recommended conditions (primarily -20 °C where indicated). Components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eBroad MVM strain coverage:\u003c\/strong\u003e Targets and detects four MVM strains (i, p, m and c).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Reported limit of detection of 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification with common mammalian cell lines or tested microbial genomes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal process control included:\u003c\/strong\u003e VIR Internal Control (IC) monitors extraction and PCR inhibition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSupplied controls:\u003c\/strong\u003e MVM Positive Control provided (reference product number supplied separately); NTC and NCS workflows specified.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with automated extraction:\u003c\/strong\u003e Recommended for use with rHCDpurify System and the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit to efficiently recover viral DNA from complex matrices.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Pre-mixed MVM primer \u0026amp; probe simplifies setup and reduces assay variability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eValidated qPCR program:\u003c\/strong\u003e Predefined cycling conditions and instrument setup guidance provided for common platforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eMolecular evaluation of industrial cell lines (e.g., CHO, BHK, NS0) utilized in biopharmaceutical production\u003c\/li\u003e\n\u003cli\u003eAnalysis of cell culture supernatant and in vitro cell culture supplements for MVM target sequences\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis support within in vitro research environments\u003c\/li\u003e\n\u003cli\u003eIn vitro research studies requiring the qualitative molecular analysis of MVM target sequences\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (Part No. NNA057) — 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Master MIX (Part No. NNB023) — 850 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMVM Primer \u0026amp; Probe MIX (Part No. NNC106) — 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (Part No. NNC107) — 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only — consult compliance statement.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction in a biological safety cabinet and follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and certified reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\n\u003cp\u003e• Avoid cross-contamination: use separate areas and aerosol-resistant tips for reagent preparation, sample handling, and amplification.\u003c\/p\u003e\n\u003cp\u003e• If VIC internal control Ct of sample is \u0026gt;2 cycles different from NCS VIC Ct, suspect PCR inhibition; retest or treat sample to remove inhibitors.\u003c\/p\u003e\n\u003cp\u003e• Check expiration dates on component labels and store components under appropriate conditions (see labels).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506742.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506742.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the SHENTEK MVM Detection Kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe reported analytical sensitivity is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which MVM strains does this kit detect?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed to detect MVM strains i, p, m and c.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What sample types are supported and what extraction is recommended?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for cell culture and serum samples (example: 200 µL sample input). Use the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730) and rHCDpurify System for nucleic acid extraction as recommended.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted and what indicates inhibition?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003ePositive: FAM Ct \u0026lt; 40 (at least one well) with specific amplification and VIC Ct \u0026lt; 35. Negative: FAM undetected in duplicates and Ctsample - CtNCS \u0026lt; 2. If Ctsample - CtNCS ≥ 2 (VIC comparison), this suggests PCR inhibition; retest or remove inhibitors.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189065277621,"sku":"1506742","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MVM_Detection_Kit.jpg?v=1742286893"},{"product_id":"mvm-positive-control","title":"MVM Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    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25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    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.shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003ePCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® MVM Positive Control is an inactivated, replication-defective pseudovirus intended as a process positive control for monitoring MVM DNA extraction and PCR detection workflows. It is designed for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kits and SHENTEK® MVM Detection Kit. The product is supplied as 100 μL per tube (3 tubes) and is ready for direct use without dilution. It supports verification of extraction efficiency and downstream PCR assay performance across the entire workflow.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Number\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e1506747\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormulation \/ Type\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eInactivated replication-defective pseudovirus (MVM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume \/ Quantity\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess positive control for MVM DNA extraction and PCR detection assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage temperature\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore below -65 °C; stable for up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStability\u003c\/td\u003e\n\u003ctd\u003eUp to 12 months when stored at recommended temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePreparation\u003c\/td\u003e\n\u003ctd\u003eThaw completely at 2–8 °C or on ice, vortex and spin briefly before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eWorkflow control:\u003c\/strong\u003e Monitors the entire process from nucleic acid extraction to PCR detection, enabling verification of extraction efficiency and assay performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Provided as a ready-to-use positive control that does not require dilution prior to use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStable supply format:\u003c\/strong\u003e Supplied in multiple tubes (100 μL × 3) and stable for up to 12 months under recommended storage conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety-conscious design:\u003c\/strong\u003e Inactivated, replication-defective pseudovirus provides a safer control material while recommending biosafety cabinet handling and proper waste sterilization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003ePositive control for MVM DNA extraction workflows\u003c\/li\u003e\n\u003cli\u003ePositive control for PCR detection assays (MVM)\u003c\/li\u003e\n\u003cli\u003eQuality control for nucleic acid extraction kits and PCR reagents\u003c\/li\u003e\n\u003cli\u003eAssay development, validation, and troubleshooting for MVM detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMVM Positive Control\u003c\/span\u003e\u003cstrong\u003e100 μL × 3 tubes; store below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (control: 3 tubes) | Method: PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Intended for research use only; not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the positive control sample (PCS) preparation protocol in the SHENTEK® MVM Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Use only after equilibrating to room temperature; avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Do not use reagents beyond their expiration date.\u003c\/p\u003e\n\u003cp\u003e• Although inactivated and replication-defective, operate in a biosafety cabinet and autoclave experimental waste prior to disposal in compliance with medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506747.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506747.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the SHENTEK® MVM Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIt is intended as a process positive control to monitor MVM DNA extraction and PCR detection assays when used with SHENTEK® extraction and detection kits.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the MVM Positive Control be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore below -65 °C. The product is stable for up to 12 months when stored at the recommended temperature; check the label for the expiration date.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Do I need to dilute the positive control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is provided ready for direct use without dilution. Thaw at 2–8 °C or on ice, vortex and spin briefly prior to use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is this product safe to handle on the bench?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe product is an inactivated replication-defective pseudovirus, which reduces risk, but it is still recommended to handle it in a biosafety cabinet and to autoclave and properly dispose of experimental waste.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg fill=\"currentColor\" viewbox=\"0 0 20 20\" class=\"shentek-notice-icon\"\u003e\n                \u003cpath clip-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" fill-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189069602997,"sku":"1506747","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MVM_Positive_Control.jpg?v=1742287016"},{"product_id":"vesivirus-2117-detection-kit-shentek®","title":"Vesivirus 2117 Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Vesivirus 2117 Analysis Kit is a qualitative RT‑qPCR assay for Analysis of Vesivirus 2117 contamination in mammalian cell lines (e.g., CHO) and related sample matrices used in biopharmaceutical production. The kit is designed to be used together with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730). The assay includes a Vesivirus 2117 Positive Control and a VIR Internal Control (IC) to monitor extraction and amplification. The method achieves a sensitivity of 50 copies per reaction, demonstrates high specificity without cross‑amplification of a range of cell line and microbial genomes, and is suitable for detecting viral RNA from complex matrices such as cells and serum. For Research Use Only.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Vesivirus 2117 RNA in mammalian cell lines and related samples (e.g., cell culture, bovine serum).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eSensitivity of 50 copies per reaction (reported assay sensitivity).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eHigh specificity; no cross‑amplification observed with genomes from CHO, VERO, 293T, MDCK, NS0, Sf9, E. coli, Pichia pastoris and other tested bovine\/porcine\/engineering cell lines.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample types\u003c\/td\u003e\n\u003ctd\u003eCell culture (≤10^7 cells), bovine serum, and other matrices amenable to SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction format \/ volume\u003c\/td\u003e\n\u003ctd\u003e20 µL total reaction volume (10 µL RT‑qPCR MIX + 10 µL sample\/controls); per reaction RT‑qPCR MIX composition provided (10 µL final MIX).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT‑qPCR mix composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003e5×RT‑qPCR Buffer 4 µL, RT‑qPCR Enzyme MIX 1 µL, VV‑2117 Primer\u0026amp;Probe MIX 3.3 µL, VIR IPC MIX 1.7 µL, (optionally) VIR Internal Control (IC) 0.5 µL; final volume 10 µL RT‑qPCR MIX.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C for 15:00 (1 cycle); Activation 95 °C for 0:30 (1 cycle); 40 cycles of 95 °C for 0:15 and 60 °C for 1:00 (fluorescence read during 60 °C).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance \/ interpretation thresholds\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eControl acceptance: FAM and VIC Ct \u0026lt; 35.0 for PCS; NTC and NCS: undetected in FAM and Ct \u0026lt; 35.0 in VIC. Test sample calls: FAM Ct \u0026lt; 40.0 (at least one replicate) with specific amplification = Positive. Negative defined as undetected FAM and VIC sample Ct difference to NCS (Ctsample − CtNCS) \u0026lt; 2. If Ctsample − CtNCS ≥ 2, result is not conclusive (possible inhibition).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls included \/ recommended\u003c\/td\u003e\n\u003ctd\u003eVesivirus 2117 Positive Control (PCS) — separate product (1506743‑R01) recommended; VIR Internal Control (IC) and VIR IPC MIX included to monitor extraction\/amplification. No Template Control (NTC) and Negative Control Sample (NCS) required.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months; check expiration on labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended instruments\u003c\/td\u003e\n\u003ctd\u003eCompatible with SHENTEK‑96S, LightCycler 480Ⅱ, ABI 7500 and other real‑time PCR systems supporting probe assays (FAM\/VIC detection).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExtraction\u003c\/td\u003e\n\u003ctd\u003eDesigned to be used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730); automated extraction on rHCDpurify system recommended but manual extraction possible per extraction kit guide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity reported at 50 copies per reaction for reliable detection of low‑level contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross‑amplification with a panel of mammalian cell lines and common engineering microorganisms tested.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal\/extraction controls:\u003c\/strong\u003e Includes VIR Internal Control (IC) and VIR IPC MIX to monitor extraction efficiency and PCR inhibition; Positive Control provided as separate product.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on SHENTEK‑96S, LightCycler 480Ⅱ and ABI 7500; general probe‑based qPCR instruments supported.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized for complex matrices:\u003c\/strong\u003e Combined workflow with SHENTEK extraction kit enables efficient recovery of viral RNA from cells and serum.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eMolecular evaluation of Vesivirus 2117 target sequences in mammalian cell substrates (e.g., CHO) utilized in biopharmaceutical manufacturing\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control and molecular monitoring of cell culture systems and in vitro cell culture supplements\u003c\/li\u003e\n\u003cli\u003eIn-process analytical evaluation and biomanufacturing root cause analysis workflows\u003c\/li\u003e\n\u003cli\u003eIn vitro research studies requiring the qualitative molecular analysis of Vesivirus 2117 target RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (NNA057) 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT‑qPCR Buffer (NNB018) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase‑Free H2O (NND008) 1.2 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (or as labelled)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT‑qPCR Enzyme MIX (NNC079) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVV‑2117 Primer \u0026amp; Probe MIX (NNC123) 170 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (NNC107) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (not for diagnostic or therapeutic use).\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate protective eyewear, gloves, mask and lab clothing.\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction steps in a biosafety cabinet and follow BSL‑2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease‑free consumables; avoid RNase\/DNase contamination.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit reagents completely at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\n\u003cp\u003e• Protect enzyme mixes from light; store components at recommended temperatures and check expiration dates on labels.\u003c\/p\u003e\n\u003cp\u003e• Include NTC, NCS and PCS controls and run duplicates as recommended; follow acceptance criteria for Ct interpretation.\u003c\/p\u003e\n\u003cp\u003e• If inhibition is suspected (VIC Ct difference ≥ 2 vs NCS), retest after removing inhibitors or repeat extraction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506743.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506743.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is reported at 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for mammalian cell culture samples (e.g., CHO cells, ≤10^7 cells) and bovine serum when used with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit; other complex matrices may be used following appropriate extraction and validation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret Ct values?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eA sample is positive when FAM Ct \u0026lt; 40.0 (at least one replicate) with specific amplification. PCS, NCS and NTC acceptance criteria and VIC internal control thresholds are provided in the user guide; if the VIC Ct difference indicates inhibition (Ctsample − CtNCS ≥ 2), the result is not conclusive and re‑extraction or inhibitor removal is recommended.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at −20 °C as indicated on labels. Components are stable for up to 24 months under appropriate storage conditions; always check the expiration date on tube labels.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189071569077,"sku":"1506743","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Vesivirus_2117_Detection_Kit.jpg?v=1742287223"},{"product_id":"vesivirus-2117-positive-control-shentek®","title":"Vesivirus 2117 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Vesivirus 2117 Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of Vesivirus 2117 target sequences in mammalian cell lines (e.g., CHO) and related sample matrices utilized in biopharmaceutical production. The kit is optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730). The assay includes a Vesivirus 2117 Positive Control and an Internal Process Control (IPC) to monitor nucleic acid recovery and amplification. The method achieves a limit of testing (LoD) of 50 copies per reaction, demonstrates high analytical specificity without cross-amplification against a range of cell line and non-target genomes, and is highly suitable for the analysis of target RNA from complex matrices such as cell suspensions and in vitro cell culture supplements. Strictly for Industrial Bioprocessing Quality Control and In Vitro Analytical Use Only.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eForm\u003c\/td\u003e\n\u003ctd\u003eArmored RNA positive control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage temperature and stability\u003c\/td\u003e\n\u003ctd\u003eStored below -65 °C; stable up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess control for Vesivirus 2117 RNA extraction and RT‑PCR detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003eNot specified in the document\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilution requirement\u003c\/td\u003e\n\u003ctd\u003eReady for direct use without dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHandling\u003c\/td\u003e\n\u003ctd\u003eHandle in a biosafety cabinet; avoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProcess monitoring:\u003c\/strong\u003e Monitors the entire Vesivirus 2117 RNA extraction and RT‑PCR workflow (extraction → detection).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady to use:\u003c\/strong\u003e Provided as a ready-to-use positive control; no dilution required before use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA:\u003c\/strong\u003e Stabilized armored RNA format improves nuclease resistance and mimics viral particles during extraction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term stability:\u003c\/strong\u003e Stable when stored below -65 °C for up to 12 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety guidance:\u003c\/strong\u003e Intended to be used in a biosafety cabinet; experimental waste must be autoclaved prior to disposal.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eMolecular evaluation of Vesivirus 2117 target sequences in mammalian cell substrates (e.g., CHO) utilized in biopharmaceutical manufacturing\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control and molecular monitoring of cell culture systems and in vitro cell culture supplements\u003c\/li\u003e\n\u003cli\u003eIn-process analytical evaluation and biomanufacturing root cause analysis workflows\u003c\/li\u003e\n\u003cli\u003eIn vitro research studies requiring the qualitative molecular analysis of Vesivirus 2117 target RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVesivirus 2117 Positive Control\u003c\/span\u003e\u003cstrong\u003eBelow -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 3 tubes (100 μL each) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• This product is intended for research use only and is not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex and spin briefly prior to use.\u003c\/p\u003e\n\u003cp\u003e• Follow the positive control sample (PCS) preparation protocol in the SHENTEK® Vesivirus 2117 Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• Product is ready for direct use without dilution; equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cp\u003e• Avoid repeated freeze-thaw cycles and do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Do not use reagents beyond their expiration date; check label for expiry.\u003c\/p\u003e\n\u003cp\u003e• Handle armored RNA material in a biosafety cabinet; sterilize experimental waste by autoclaving and dispose of per medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506743-R01.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506743-R01.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is the Vesivirus 2117 Positive Control ready to use or does it require dilution?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe product is ready for direct use without dilution. Thaw as instructed, vortex and spin briefly before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the positive control be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore below -65 °C. The product can be stored under appropriate conditions for up to 12 months; check the label for the expiration date.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this positive control be used for clinical diagnostic purposes?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for Research Use Only (RUO) and is not for clinical diagnosis or treatment.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What biosafety and waste disposal precautions are required when using this product?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eHandle the armored RNA positive control in a biosafety cabinet. Experimental waste must be sterilized via autoclaving and disposed of in compliance with medical waste regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189073436853,"sku":"1506743-R01","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Vesivirus_2117_Positive_Control.jpg?v=1742287522"},{"product_id":"porcine-circovirus-pcv-1-detection-kit-shentek®","title":"Porcine Circovirus (PCV-1) Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® PCV-1 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Porcine Circovirus Type 1 (PCV-1) contamination in cell banks, cell culture, trypsin and related biological matrices\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction format \/ total volume\u003c\/td\u003e\n\u003ctd\u003e30 µL reaction volume per well (20 µL qPCR MIX + 10 µL sample\/DDB\/controls)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eqPCR Master MIX 15.9 µL; PCV-1 Primer \u0026amp; Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (add VIR Internal Control 0.5 µL to qPCR MIX if not added during extraction) — total qPCR MIX = 20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation 95 °C 10:00 (mm:ss) ×1; Denaturation 95 °C 00:15; Annealing\/Extension 60 °C 01:00; 40 cycles (instrument reads fluorescence during annealing\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — controls (Ct thresholds)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM undetected; VIC undetected or absence of specific amplification. NCS: FAM undetected; VIC Ct \u0026lt; 35. PCS: FAM Ct \u0026lt; 35 and VIC Ct \u0026lt; 35 (specific amplification for duplicate runs).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — test samples\u003c\/td\u003e\n\u003ctd\u003ePositive: FAM Ct \u0026lt; 40 (at least one well) with specific amplification and VIC Ct \u0026lt; 35 for duplicates. Negative: FAM undetected and VIC present (Ctsample - CtNCS \u0026lt; 2). Not conclusive \/ inhibition: Ctsample - CtNCS ≥ 2 or inhibited VIC signal; repeat extraction and assay or remove inhibitors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification observed with genomes from bovine, porcine, and multiple engineered cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineered bacteria\/yeast (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample input guidance\u003c\/td\u003e\n\u003ctd\u003eCell culture: collect 100–200 µL sample (≤10^7 cells) and proceed with pretreatment and extraction. Pretreatment uses Pretreatment buffer at 1:10 (sample:buffer), RT 5 min, centrifuge 450 × g 30 min at 4 °C; Proteinase K digestion: add 100 µL Proteinase K Buffer, 50 µL Proteinase K, 10 µL VIR IC; incubate 55 °C for 30 min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended extraction\u003c\/td\u003e\n\u003ctd\u003eUse SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730); automated extraction (rHCDpurify system) recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500 (and other real-time PCR systems supporting probe assays and FAM\/VIC detection)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction enabling sensitive detection of low-level contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Designed primers\/probes show no cross-amplification with common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or with E. coli and Pichia pastoris genomes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal control included:\u003c\/strong\u003e VIR Internal Control monitors extraction efficiency and PCR inhibition to ensure reliable results and help identify inhibited samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized workflow:\u003c\/strong\u003e Pre-treatment, proteinase K digestion and recommended extraction workflow provided; compatible with automated extraction (rHCDpurify) and common qPCR instruments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated primer\/probe mix and qPCR Master Mix reduce setup variability and simplify assay preparation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eValidated for complex matrices:\u003c\/strong\u003e Combined with SHENTEK extraction kit, the assay efficiently recovers PCV-1 DNA from complex matrices such as cells and serum.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of PCV-1 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for PCV-1 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing PCV-1 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePCV-1 Primer \u0026amp; Probe MIX (Part No. NNC121) 120 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Master MIX (Part No. NNB023) 850 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (Part No. NNC107) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (Part No. NNA057) 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedures in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free, RNase\/DNase-free consumables and certified sterile tubes and tips.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer\/probe mixes from light.\u003c\/p\u003e\n\u003cp\u003e• Store reagents at recommended temperatures (–20 °C) and check expiration dates; kit components stable up to 24 months.\u003c\/p\u003e\n\u003cp\u003e• If VIC internal control (IC) signal is inhibited, repeat sample preparation or remove inhibitors and retest.\u003c\/p\u003e\n\u003cp\u003e• Follow instrument-specific settings and plate layouts; validate assay performance as required by your laboratory.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506744.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506744.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's limit of detection (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay limit of detection is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types can be tested with this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for detection of PCV-1 in cell banks, cell culture samples, trypsin and serum when combined with the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit. Sample pretreatment and extraction steps are provided in the extraction kit manual.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the required controls for each run?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude a No Template Control (NTC), a Negative Control Sample (NCS), and a Positive Control Sample (PCS). Duplicates are recommended for controls and test samples. Acceptance criteria and Ct thresholds are provided in the user guide.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the VIR internal control (VIC) signal is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf VIC signal is inhibited, reprocess the sample (repeat extraction) or apply additional inhibitor-removal steps, then repeat the qPCR. Inhibited VIC indicates possible PCR inhibition in the sample.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189076418741,"sku":"1506744","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Porcine_Circovirus_PCV-1_Detection_Kit.jpg?v=1742287594"},{"product_id":"porcine-circovirus-pcv-2-detection-kit-shentek®","title":"Porcine Circovirus (PCV-2) Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® PCV-1 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Porcine Circovirus Type 2 (PCV-2) contamination in cell banks, cell culture, trypsin and serum (research use only).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 reactions (Product No. 1506745).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection chemistry \/ format\u003c\/td\u003e\n\u003ctd\u003eProbe-based real-time quantitative PCR (qPCR), dual-channel reporting (FAM for PCV-2 target, VIC for internal control).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\n\u003ctd\u003ePrepare qPCR MIX then combine 20 µL qPCR MIX + 10 µL sample (extracted nucleic acid or control) for a 30 µL final reaction volume.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eqPCR Reaction Buffer 15.9 µL; PCV-2 Primer \u0026amp; Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (if VIR Internal Control not added during extraction, add 0.5 µL VIR Internal Control to MIX). Total qPCR MIX volume: 20 µL (test samples).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation: 95 °C 10:00 (mm:ss) ×1; Denaturation: 95 °C 00:15; Annealing\/Extension: 60 °C 01:00; 40 cycles. Fluorescence read during annealing\/extension.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance \/ result interpretation thresholds\u003c\/td\u003e\n\u003ctd\u003eControls: NTC — FAM undetected; VIC Ct \u0026lt; 35 or undetected depending on control. Test sample positive: FAM Ct \u0026lt; 40 (specific amplification) and VIC Ct \u0026lt; 35 for duplicates. Negative: FAM undetected and Ctsample - CtNCS \u0026lt; 2. If Ctsample - CtNCS ≥ 2 or VIC inhibited, result not conclusive and repeat extraction or remove inhibitors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification observed with genomes from bovine, porcine, cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S Real-time PCR System; LightCycler 480 II; ABI 7500 Real-Time PCR System; other qPCR instruments supporting probe-based assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable up to 24 months; follow label expiry and protect primer\/probe mix from light).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls provided \/ required\u003c\/td\u003e\n\u003ctd\u003eNo Template Control (NTC), Negative Control Sample (NCS), Positive Control Sample (PCS) recommended; VIR Internal Control provided to monitor extraction\/PCR inhibition.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended sample types\u003c\/td\u003e\n\u003ctd\u003eCell culture (≤10^7 cells per 100–200 µL), bovine serum, trypsin and other biological matrices (use SHENTEK Virus DNA \u0026amp; RNA Extraction Kit for sample prep).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction enabling sensitive detection of PCV-2 contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification reported with multiple mammalian cell lines and common engineering microbes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated internal control:\u003c\/strong\u003e VIR Internal Control monitors extraction efficiency and PCR inhibition (VIC channel).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, LightCycler 480 II, ABI 7500) and adaptable to other probe-based qPCR instruments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConvenient workflow:\u003c\/strong\u003e Ready-to-use primer\/probe mix and supplied reaction buffer simplify setup; designed to be used with SHENTEK Virus DNA \u0026amp; RNA Extraction Kit for robust sample processing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of PCV-2 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for PCV-2 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing PCV-2 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePCV-2 Primer \u0026amp; Probe MIX (Part No. NNC122) 120 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (Part No. NNC107) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (Part No. NNA057) 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For research use only (RUO). Not for human or diagnostic clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, laboratory clothing).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedure in a biosafety cabinet; follow BSL‑2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and reagents. Avoid cross-contamination between samples, controls and reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• If VIC internal control signal is inhibited, repeat sample preparation or remove inhibitors and re-run the assay.\u003c\/p\u003e\n\u003cp\u003e• Store kit components according to label and check expiration date; items recommended at ≤ -20 °C should be stored within ±10 °C (–30 °C to –10 °C) per USP guidance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506745.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506745.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay limit of detection (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe reported limit of detection for the SHENTEK® PCV-2 Detection Kit is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eRecommended sample types include cell culture (100–200 µL, ≤10^7 cells), bovine serum and similar biological matrices. Use the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for sample preparation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret an inhibited internal control (VIC) signal?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf the VIC internal control is inhibited (reduced or absent VIC signal), this indicates potential PCR inhibitors or extraction failure. Repeat sample preparation, consider further purification to remove inhibitors, and re-run the assay.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should kit components be stored and what is shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore components as indicated on the labels, generally at -20 °C. Kit components are stable for up to 24 months; always check the expiration date on the product labels.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg fill=\"currentColor\" viewbox=\"0 0 20 20\" class=\"shentek-notice-icon\"\u003e\n                \u003cpath clip-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" fill-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189096407221,"sku":"1506745","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Porcine_Circovirus_PCV-2_Detection_Kit.jpg?v=1742287945"},{"product_id":"kanamycin-elisa-kit-shentek®","title":"Kanamycin ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2 - 8 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis is an indirect-competitive ELISA kit for quantitative determination of kanamycin residues in plasmid DNA and related biologic samples (including cell and gene therapy products). Microplate wells are coated with kanamycin antigen which competes with kanamycin in the sample for a monoclonal anti-kanamycin antibody. An enzyme-tagged secondary antibody binds the primary antibody; addition of TMB chromogenic substrate yields a colorimetric signal at 450 nm that is inversely proportional to kanamycin concentration. Total kanamycin is determined from a standard curve (4-parameter logistic) and adjusted by sample dilution factor. Sample suitability testing is recommended for other biologic matrices to assess matrix interference.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eIndirect-competitive ELISA, 96-well plate (8×12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e\u0026lt; 0.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 ng\/mL - 16 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAccuracy (Spike recovery)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e80% - 120%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePrecision\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCoefficient of variation (CV) \u0026lt; 20%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCross-reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eKanamycin 100%; Streptomycin \u0026lt;1%; Ampicillin \u0026lt;1%; Chloramphenicol \u0026lt;1%; Tetracycline \u0026lt;1%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve \u0026amp; modeling\u003c\/td\u003e\r\n                        \u003ctd\u003eStandard curve built from ST1-ST7; concentrations: 0.5–32 ng\/mL (serial dilutions); 4-parameter logistic regression (4PL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended incubation temperature\u003c\/td\u003e\r\n                        \u003ctd\u003e25 °C ± 3 °C (optimum)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at 2 - 8 °C; overall kit stable for up to 12 months. Working solutions prepared fresh and used same day.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit below 0.5 ng\/mL, suitable for trace-level kanamycin residue analysis.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eGood accuracy and precision:\u003c\/strong\u003e Spike recovery 80–120% and CV \u0026lt;20% across tested runs.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Minimal cross-reactivity with other common antibiotics (most \u0026lt;1%).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete 96-test kit:\u003c\/strong\u003e Includes kanamycin-coated plate, standards, primary and enzyme-conjugated secondary antibodies, substrate and buffer reagents for 96 determinations.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Standardized procedures with 4-parameter logistic quantitation and recommended plate handling to support reproducible results.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOptimized for biologics:\u003c\/strong\u003e Specifically intended for kanamycin quantitation in plasmid DNA and cell\/gene therapy product workflows; sample suitability testing recommended for other matrices.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of kanamycin residues in plasmid DNA\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control of cell and gene therapy products for residual kanamycin\u003c\/li\u003e\r\n                    \u003cli\u003eResearch studies requiring measurement of kanamycin contamination in biologic preparations\u003c\/li\u003e\r\n                    \u003cli\u003eScreening of other biologic matrices for kanamycin after sample suitability testing\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard (PNB001) — 2 bottles\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard Reconstitution Diluent (PNC001) — 2 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard Solution Diluent (PNE001) — 1 × 20 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× Kanamycin Primary Antibody (PNG001) — 1 × 60 μL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× Enzyme-conjugated Secondary Antibody (PNH001) — 1 × 120 μL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin-coated Plate (PNA001) — 8 wells × 12 strips\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e10× Wash Buffer (PNJ001) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eChromogenic Substrate (TMB) (PND001) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI001) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 2 pieces\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/kit | Method: ELISA (indirect-competitive)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO); not for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at 2 - 8 °C. Do not freeze any kit components.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect kanamycin standard, secondary antibody and chromogenic substrate from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare kanamycin standard solutions and enzyme-conjugated secondary antibody working solutions on the day of use; do not store working solutions for reuse.\u003c\/p\u003e\r\n                    \u003cp\u003e• Allow kit to equilibrate at room temperature (≈25 °C) for 30 minutes before use; mix reagents thoroughly and avoid foaming.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain incubation temperature at 25 ± 3 °C; temperatures below 20 °C reduce absorbance and sensitivity.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not allow wells to dry between steps; proper washing is critical—wash 4 times with 350 μL 1× wash buffer per well.\u003c\/p\u003e\r\n                    \u003cp\u003e• Return unused strips to original foil bag with desiccant and seal tightly; strips stored at 2 - 8 °C up to 30 days after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is caustic—wear eye protection and avoid contact with skin or eyes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use kit past expiration date and do not mix reagents from different lots.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1401402.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1401402.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are recommended for this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation of kanamycin residues in plasmid DNA and is suitable for cell and gene therapy product samples. For other biologic matrices, run a sample suitability (matrix interference) test and use spiked recovery to validate the assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe detection limit is \u0026lt; 0.5 ng\/mL and the working detection range is 1 ng\/mL to 16 ng\/mL (quantitation using the standard curve).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should standards and working solutions be prepared and stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare the kanamycin stock and serial standards using the provided diluents per the manual. Stock standard solution and working solutions should be prepared fresh on the day of use; stock standard can be stored at 2-8 °C up to 30 days if protected from light. Do not store working solutions for reuse.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How is the result calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCalculate absorbance (%) relative to the zero standard, construct a standard curve (4-parameter logistic) from ST1–ST7, derive sample concentration from the curve and multiply by dilution factor. Note OD is inversely correlated with kanamycin concentration.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — not intended for clinical use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189174624437,"sku":"1401402","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KanamycinELISAKit.jpg?v=1742291778"},{"product_id":"bovine-serum-albumin-bsa-elisa-kit-shentek®","title":"Bovine Serum Albumin (BSA) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit; calibration standard stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSolid-phase double-antibody sandwich ELISA for quantitative detection of residual bovine serum albumin (BSA). Microtiter strips are pre-coated with affinity-purified sheep anti-BSA capture antibody. Samples and calibration standards are incubated in the coated wells, followed by a biotinylated sheep anti-BSA detection antibody and streptavidin–HRP. TMB substrate is used for color development (blue product, max absorbance ~655 nm) and reaction is stopped to yield a yellow product read at 450 nm (with 620–650 nm reference). OD450 correlates with BSA concentration and sample results are calculated using a 4-parameter logistic (4-PL) calibration curve.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003e96-well microtiter plate strips, double-antibody sandwich ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e96 tests\/Kit (8-well × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample volume per well\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1–32 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCross-reactivity with host cell proteins (HEK293T, MDCK, CHO, BL21, Vero) \u0026lt; 0.1%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model \/ Example parameters\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL). Example: A=3.14274; B=-1.10808; C=36.73888; D=0.00859; R²=0.99999\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRead wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003ePrimary 450 nm, reference 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample storage\u003c\/td\u003e\r\n                        \u003ctd\u003eFor long-term storage, -70 °C or below recommended to avoid degradation; avoid repeated freeze-thaw cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation examples\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation 2 h RT; biotinylated antibody 45 min RT; streptavidin-HRP 30 min RT; TMB reaction 15 min RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume for dilutions\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume 5 µL recommended to avoid pipetting error\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 1 ng\/mL enables detection of low-level residual BSA in bioprocess samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal anti-BSA capture\/detection antibodies with cross-reactivity to common host cell proteins \u0026lt;0.1%.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use pre-coated strips:\u003c\/strong\u003e Anti-BSA coated microtiter strips supplied in sealed bag with desiccant for reduced setup time and consistent performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated 4-PL calibration:\u003c\/strong\u003e Use of 4-parameter logistic curve for accurate quantitation across the stated dynamic range.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete reagent set:\u003c\/strong\u003e Includes calibration standard, wash buffer concentrate, biotinylated antibody, streptavidin-HRP, TMB substrate and stop solution.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOptimized workflow:\u003c\/strong\u003e Standardized incubation and wash steps for reproducible results and straightforward integration into QC workflows.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual BSA in in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eMeasurement of BSA in harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and purification monitoring in biologics manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing for residual serum-derived impurities\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBSA Calibration standard (PNB007)\u003c\/span\u003e\u003cstrong\u003e1 × 500 µL; store at 2-8 °C (refer to tube label); stable up to 1 year\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-BSA Microtiter Strips (PNA007)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated, sealed in bag with desiccant; store at 2-8 °C, opened strips in bag with desiccant up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultra-pure water to prepare 1×Wash Buffer; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-BSA: Biotinylated Conjugate (100×) (PNG007)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; biotinylated anti-BSA (sheep polyclonal) in protein matrix with preservative; sealed, keep away from light; dilute 100× in 1×Wash Buffer before use; centrifuge before use; recommended single-use, prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002)\u003c\/span\u003e\u003cstrong\u003e1 × 140 µL; streptavidin labeled with HRP; sealed, keep away from light; dilute 100× in 1×Wash Buffer before use; centrifuge before use; recommended single-use, prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND005)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; 3,3',5,5'-tetramethylbenzidine; sealed, keep away from light; equilibrate to RT 20 min before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; store at 2-8 °C; handle with appropriate PPE (goggles, gloves)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubations to prevent evaporation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2-8 °C; check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid BSA contamination of the workspace and consumables; perform assay in BSA-free environment.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra-pure water; water temperature should not exceed 37 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inhibits HRP and will cause underestimation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid direct contact with skin, eyes and clothing; wear goggles and appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Anti-BSA:Biotinylated Conjugate and Streptavidin-HRP fresh before use and centrifuge concentrates before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless before use; discard and contact support if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not allow wells to dry during the assay; seal plate to prevent evaporation after incubations.\u003c\/p\u003e\r\n                    \u003cp\u003e• Plate reading should be completed within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended volume for accurate dilution steps is 5 µL; pipette carefully to avoid bubbles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended to validate the method for each sample matrix prior to routine testing.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1401401.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1401401.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay dynamic range and LLOQ?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 1–32 ng\/mL with an LLOQ of 1 ng\/mL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is sample concentration calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSubtract NCS OD from sample OD, use a 4-parameter logistic (4-PL) regression of the calibration standards to derive concentration, then multiply by sample dilution factor.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I store opened components and prepared reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore unopened kit at 2–8 °C. Opened strips: keep sealed with desiccant at 2–8 °C up to 30 days. Calibration standard: 2–8 °C up to 1 year. Prepare diluted detection reagents fresh and use immediately; biotinylated antibody and streptavidin-HRP are recommended single-use due to stability.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with different sample matrices (process intermediates, final drug product)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — the kit is intended for in-process samples, harvested bulk, drug substance and drug product. A method validation is recommended for each matrix to confirm suitability, appropriate dilutions, and recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189190615221,"sku":"1401401","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BovineSerumAlbumin_BSA_ELISAKit.jpg?v=1742292802"},{"product_id":"porcine-trypsin-elisa-kit-shentek®","title":"Porcine Trypsin ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit); Reconstituted standard: \u0026lt;-20 °C (up to 90 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003ePorcine Trypsin ELISA Kit is a solid-phase, double-antibody sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative detection of residual porcine trypsin in biological products and downstream purification samples. A sheep polyclonal anti-trypsin capture antibody is coated on microtiter strips. Samples and calibration standards are added to the coated wells, followed by a biotinylated anti-trypsin detection antibody and Streptavidin-HRP. HRP catalyzes TMB oxidation to produce a colorimetric signal (read at 450 nm with a 620–650 nm reference). The kit includes lyophilized calibration standards, pre-coated strips, reagents for detection (biotinylated antibody and Streptavidin-HRP), TMB substrate and stop solution. The assay is intended for research use only to detect residual recombinant or pancreas-derived porcine trypsin in harvested bulk, in-process samples, drug substance and drug product.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat\u003c\/td\u003e\r\n                        \u003ctd\u003eSandwich ELISA (solid-phase)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample volume per well\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.040–2.56 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.04 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards (nominal concentrations)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 2.56 ng\/mL, ST2 1.28 ng\/mL, ST3 0.64 ng\/mL, ST4 0.32 ng\/mL, ST5 0.16 ng\/mL, ST6 0.08 ng\/mL, ST7 0.04 ng\/mL, NCS 0 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model (example)\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL); example fit parameters provided (A=2.16282, B=-1.00528, C=1.54458, D=-0.01333, R²=0.99996)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, MDCK, Vero, HEK293, E. coli and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate washing\u003c\/td\u003e\r\n                        \u003ctd\u003eUse 1× Wash Buffer; typical wash volume 340 µL\/well; wash 3 times between steps (5 times before TMB)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation times (typical per protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 1 hour at RT (500 rpm); Anti-Trypsin:Biotin incubation: 45 min at RT; Streptavidin-HRP incubation: 30 min at RT; TMB incubation: 15 min at RT; post-stop incubation: 5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate layout \/ replicates\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommend 2–3 replicates per sample and inclusion of spiked recovery controls\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage of kit\u003c\/td\u003e\r\n                        \u003ctd\u003eStore kit at 2–8 °C. Check expiration date on labels.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage of opened components\u003c\/td\u003e\r\n                        \u003ctd\u003eAnti-Trypsin microtiter strips: 2–8 °C (in bag with desiccant) up to 90 days; Reconstituted Trypsin Calibration Standard: \u0026lt; -20 °C up to 90 days (≤3 freeze-thaw cycles)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH recommendation for samples\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommended sample pH 6.0–8.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 0.04 ng\/mL and linear range down to 0.04 ng\/mL enables detection of low residual trypsin levels.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal anti-trypsin antibodies with no observed cross-reactivity to common host cell lines and microorganisms (CHO, MDCK, Vero, HEK293, E. coli, Hansenula).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit format:\u003c\/strong\u003e Includes pre-coated strips, lyophilized standards, detection reagents (biotinylated antibody, Streptavidin-HRP), TMB substrate and stop solution for a 96-test kit.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQuantitative readout:\u003c\/strong\u003e 4-parameter logistic calibration recommended for accurate quantitation; absorbance read at 450 nm with reference wavelength.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for downstream process monitoring:\u003c\/strong\u003e Suitable for testing residual recombinant porcine trypsin and pancreas-derived trypsin in in-process and final product samples.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eDetermination of residual porcine trypsin in downstream purification of biologics\u003c\/li\u003e\r\n                    \u003cli\u003eTesting harvested bulk, in-process samples, drug substance and drug product for residual trypsin\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and validation for recombinant protein production where trypsin is used (e.g., cell dissociation, activation steps)\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications requiring quantitation of porcine trypsin contamination\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTrypsin Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003ePNB006, 2 bottles; store lyophilized per label; reconstituted standard \u0026lt; -20 °C (up to 90 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003ePNA006, 8 wells × 12 strips; store in vacuumed bag with desiccant at 2–8 °C; use within 90 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003ePNC003, 1 × 240 μL; room temperature handling; used to dissolve calibration standard\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003ePNF001, 3 × 25 mL; dilute 10× with ultra-pure water to 1×; store 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin:Biotin (100×)\u003c\/span\u003e\u003cstrong\u003ePNG006, 1 × 140 μL; centrifuge before use; dilute 100× in 1× Wash Buffer; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×)\u003c\/span\u003e\u003cstrong\u003ePNH002, 1 × 140 μL; centrifuge before use; dilute 100× in 1× Wash Buffer; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003ePND002, 1 × 12 mL; equilibrate to RT 20 min before use; TMB should be colorless\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003ePNI002, 1 × 6 mL; corrosive — avoid contact with eyes, skin, clothing\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003ePNK001, 3 pieces; use to prevent evaporation during incubations\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C. Check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard: store below -20 °C up to 90 days; avoid more than 3 freeze-thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Anti-Trypsin microtiter strips: store in sealed bag with desiccant at 2–8 °C; use within 90 days after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1× Wash Buffer freshly by diluting 10× concentrate with ultra-pure water; if cloudy, warm at 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Dilute Anti-Trypsin:Biotin and Streptavidin-HRP 100× in 1× Wash Buffer immediately before use; centrifuge concentrates before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use TMB substrate only if colorless; equilibrate to RT for 20 minutes before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive. Avoid contact with skin, eyes and clothing; handle in appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it will deactivate HRP and cause underestimation of trypsin levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommend sample pH between 6.0 and 8.0; outside this range may produce abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw of samples; long-term storage recommended at -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix different kit lots in the same assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain accurate pipetting (minimum recommended volume 5 µL for dilutions) and avoid bubbles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Trypsin Calibration Standard Solutions, Biotinylated Antibody Solution and Streptavidin-HRP Solution are recommended for single use due to stability concerns.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402422.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402422.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's linear range and sensitivity?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.040–2.56 ng\/mL with an LLOQ of 0.04 ng\/mL (example R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store opened strips and reconstituted standards?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore opened anti-trypsin microtiter strips in the sealed bag with desiccant at 2–8 °C for up to 90 days. Reconstituted Trypsin Calibration Standard should be stored below -20 °C for up to 90 days and subjected to no more than three freeze-thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What if I observe high background or abnormal OD values?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCommon causes include cross-contamination, insufficient washing, equipment contamination, or environmental contamination. Use freshly prepared reagents, increase wash volume or wash cycles, clean equipment with 75% ethanol, and ensure proper plate sealing and handling to reduce background.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which sample types and pH are suitable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSuitable for harvested bulk, in-process samples, drug substance and drug product. Recommended sample pH is between 6.0 and 8.0. Remove insoluble material by centrifugation or filtration and avoid repeated freeze-thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189198905525,"sku":"1402422","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BovineSerumAlbumin_BSA_ELISAKit_1da1a515-db19-44a3-aedc-3186ca1e5b60.jpg?v=1742293460"},{"product_id":"trypsin-analog-elisa-kit-shentek®","title":"Trypsin-analog ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n    \u003cdiv class=\"shentek-header-bar\"\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003e2 hours\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (check label for expiration)\u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n        \u003ch2 class=\"shentek-title\"\u003e\n            \n            Product Description\n        \u003c\/h2\u003e\n        \u003cp class=\"shentek-text\"\u003eOne-step, solid-phase sandwich Enzyme‑linked Immunosorbent Assay (ELISA) for quantitative detection of Trypsin-analog residuals in biological product preparations and process samples. The kit uses a sheep polyclonal capture antibody coated on microtiter strips and an HRP-conjugated sheep polyclonal anti-Trypsin-analog detection antibody added simultaneously with samples\/standards. HRP catalyzes TMB oxidation producing a colorimetric signal read at 450 nm (with long-wavelength correction 620–650 nm). The kit is suitable for detecting non-animal origin recombinant fungal Trypsin-analog and related engineered enzyme reagents (examples: TrypLE™, Recombinant Trypsin, HyrTryp™). Intended for research use only (not for clinical diagnostics).\u003c\/p\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\n            \u003ctable class=\"shentek-table\"\u003e\n                \u003cthead\u003e\n                    \u003ctr\u003e\n                        \u003cth\u003eParameter\u003c\/th\u003e\n                        \u003cth\u003eDetails\u003c\/th\u003e\n                    \u003c\/tr\u003e\n                \u003c\/thead\u003e\n                \u003ctbody\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003ePackage\u003c\/td\u003e\n                        \u003ctd\u003e96 tests\/Kit (8-well × 12 strips)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\n                        \u003ctd\u003eOne-step sandwich ELISA; simultaneous addition of HRP-conjugated detection antibody and samples\/standards\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.16–10.24 ng\/mL, R² \u0026gt; 0.990\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.16 ng\/mL\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eTypical read wavelengths\u003c\/td\u003e\n                        \u003ctd\u003ePrimary: 450 nm; Reference\/long-wavelength correction: 620–650 nm\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eIncubation conditions\u003c\/td\u003e\n                        \u003ctd\u003eSample incubation on thermoshaker: 600 rpm, 2 hours at room temperature; TMB substrate incubation: 15 minutes at room temperature\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eCalibration standards\u003c\/td\u003e\n                        \u003ctd\u003eSeven-point serial dilution prepared from supplied Calibration Standard to produce ST1–ST7: 10.24, 5.12, 2.56, 1.28, 0.64, 0.32, 0.16 ng\/mL (NCS = 0)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with common engineered cell lines (MDCK, Vero, HEK293, E. coli, P. pastoris, Sf9) and no cross-reactivity with recombinant human, bovine or porcine trypsin\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\n                        \u003ctd\u003epH 6.5–8.5 (outside this range may cause abnormal results)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eRecommended long-term sample storage\u003c\/td\u003e\n                        \u003ctd\u003e-70 °C to avoid degradation\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eWash buffer preparation\u003c\/td\u003e\n                        \u003ctd\u003eWash Buffer Concentrate (10×) dilute 1:10 with ultra-pure water (prepare fresh)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                \u003c\/tbody\u003e\n            \u003c\/table\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 0.16 ng\/mL allows sensitive quantification of residual Trypsin-analog.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity to common cell lines or to recombinant human\/bovine\/porcine trypsin; designed for fungal recombinant Trypsin-analog detection.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified sheep anti-Trypsin-analog polyclonal antibody for consistent capture.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step workflow:\u003c\/strong\u003e Simultaneous addition of HRP-conjugated detection antibody and samples\/standards simplifies procedure and reduces hands-on time.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eQuantitative output:\u003c\/strong\u003e Dose–response curve (4-parameter logistic) provides quantitative concentration values; compatible with standard curve software.\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003eDetection and quantification of residual Trypsin-analog in cell harvest solutions\u003c\/li\u003e\n                    \u003cli\u003eIn-process monitoring during biologics production\u003c\/li\u003e\n                    \u003cli\u003eAnalysis of drug substance and drug product for residual enzyme\u003c\/li\u003e\n                    \u003cli\u003eQuality control and method validation for products using Trypsin-analog (e.g., TrypLE™, Recombinant Trypsin, HyrTryp™)\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box\"\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eTrypsin-analog Calibration Standard (PNB009)\u003c\/span\u003e\u003cstrong\u003e1 × 100 μL; store at 2-8 °C (solution should be clear\/transparent); prepare fresh dilutions before use\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eAnti-Trypsin-analog Microtiter Strips (PNA009)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated and supplied in vacuum bag with desiccant; store sealed at 2-8 °C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eDiluent (PNE005)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2-8 °C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e1 × 25 mL; store at 2-8 °C; may crystallize at low temperature—dissolve at 37 °C before use; dilute 1:10 with ultra-pure water\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eAnti-Trypsin-analog:HRP (100×) (PNN003)\u003c\/span\u003e\u003cstrong\u003e1 × 140 μL; sheep polyclonal antibody conjugated to HRP; store at 2-8 °C; dilute 100× in Diluent immediately before use; centrifuge briefly before use\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eTMB Substrate (PND002)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; store at 2-8 °C; equilibrate to room temperature 20 min before use; protect from light\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; store at 2-8 °C; handle with care (avoid contact with eyes\/skin\/clothing)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; store at 2-8 °C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step sandwich ELISA\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\n                    \u003cp\u003e• Store kit components at 2-8 °C (including opened components); check expiration date on labels.\u003c\/p\u003e\n                    \u003cp\u003e• Recommended sample pH: 6.5–8.5; samples outside this range may produce abnormal results.\u003c\/p\u003e\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples; long-term sample storage at -70 °C is recommended.\u003c\/p\u003e\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\n                    \u003cp\u003e• Use fresh ultra‑pure water (do not exceed 37 °C) to prepare buffers; prepare Wash Buffer fresh.\u003c\/p\u003e\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it inhibits HRP and will underestimate Trypsin-analog levels.\u003c\/p\u003e\n                    \u003cp\u003e• TMB Substrate should be colorless; discard if colored. Protect TMB from light during incubation.\u003c\/p\u003e\n                    \u003cp\u003e• Stop Solution is 1 M HCl—avoid contact with eyes, skin and clothing; handle with appropriate PPE.\u003c\/p\u003e\n                    \u003cp\u003e• Centrifuge Anti-Trypsin-analog:HRP(100×) before dilution\/use to avoid loss of reagent.\u003c\/p\u003e\n                    \u003cp\u003e• Calibration Standard Solutions and HRP-conjugated antibody are recommended for single use due to stability.\u003c\/p\u003e\n                    \u003cp\u003e• Minimum recommended pipetted volume for accurate dilution: 5 μL.\u003c\/p\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n        \u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402424.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402424.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-faq\"\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay dynamic range and sensitivity?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.16–10.24 ng\/mL with an LLOQ of 0.16 ng\/mL (R² \u0026gt; 0.990).\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sample types can be tested and how should they be prepared?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTest samples include cell harvest solutions, in-process samples, drug substance and drug product. Samples must be clear and transparent; remove insoluble material by centrifugation or filtration. Dilute samples into Diluent to bring Trypsin-analog concentration within the calibration range. Avoid repeated freeze–thaw cycles.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Does the kit cross-react with other trypsins or cell components?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit shows no cross-reactivity with common engineered cell lines (MDCK, Vero, HEK293, E. coli, P. pastoris, Sf9) and no cross-reactivity with recombinant human, bovine or porcine trypsin.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are calibration standards prepared?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 100× dilute stock of the supplied Calibration Standard, then perform serial 1:1 dilutions to obtain ST1–ST7 at: 10.24, 5.12, 2.56, 1.28, 0.64, 0.32, 0.16 ng\/mL. NCS (0) is Diluent only.\u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003cdiv class=\"shentek-notice\"\u003e\n        \u003cdiv class=\"shentek-notice-header\"\u003e\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-notice-body\"\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO). Not for clinical use.\u003c\/strong\u003e – This product is intended\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n                    official compliance testing.\u003c\/p\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003etrypsin\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189205721269,"sku":"1402424","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Trypsin-analogELISAKit.jpg?v=1742294003"},{"product_id":"nonspecific-endonuclease-smne-elisa-kit-shentek®","title":"Nonspecific Endonuclease (SMNE) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 4 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (calibration standard stable for 12 months; microtiter strips stable for 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA sandwich-format solid-phase ELISA for quantitative detection of residual nonspecific endonuclease (SMNE) in bioprocess samples. The assay uses a sheep polyclonal anti-SMNE capture antibody coated on microtiter strips, a biotinylated anti-SMNE detection antibody and streptavidin-HRP. Signal is developed with TMB substrate and read at 450 nm (with 620–650 nm reference). The kit can detect genetically engineered Serratia marcescens endonucleases (e.g., Benzonase®) and is intended for research use only (not for clinical diagnostics).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eSandwich ELISA (solid-phase double-antibody sandwich)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMeasurement wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.1–25 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.25 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with RNase, host cell proteins (E. coli, CHO, HEK293T, Vero cells) or BSA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL) regression. Example curve parameters provided (A=4.08707; B=-1.00169; C=30.95401; D=0.00166; R²=0.99997)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical sample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product (samples must be clear\/transparent; remove particulates by centrifugation\/filtration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003e5 µL minimum recommended for dilution accuracy\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate layout \/ throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit (8 wells × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal antibody pair selective for SMNE; no cross-reactivity with common RNases, host cell proteins or BSA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive quantitation:\u003c\/strong\u003e Low LLOQ (0.25 ng\/mL) and linear range down to 0.1 ng\/mL for detection of residual endonuclease.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Pre-coated microtiter strips provided in a sealed bag with desiccant for convenience and reduced hands-on preparation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStandardized protocol:\u003c\/strong\u003e Complete workflow with recommended dilutions, incubation times, and 4‑PL curve fitting for reliable quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eProcess monitoring application:\u003c\/strong\u003e Designed for quantitation of SMNE in bioprocess purification stages, including detection of engineered endonucleases such as Benzonase®.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual SMNE (Serratia marcescens endonuclease) during bioprocess purification\u003c\/li\u003e\r\n                    \u003cli\u003eMonitoring endonuclease removal in in-process samples and harvested bulk\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product for residual endonuclease impurities\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and QC support during downstream processing (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSMNE Calibration standard (PNB004) 1 × 100 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (store up to 12 months; follow label instructions)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-SMNE Microtiter Strips (PNA004) 8 wells × 12 strips\u003c\/span\u003e\u003cstrong\u003e2-8 °C in sealed bag with desiccant (store up to 30 days after opening)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSMNE Diluent (PNE003) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (dilute 10× → 1× with ultra-pure water prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-SMNE: Biotinylated Conjugate (100×) (PNG004) 1 × 120 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (centrifuge before use; dilute 100× in diluent immediately prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002) 1 × 140 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; keep sealed and protected from light (dilute 100× in diluent immediately prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND003) 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; sealed and protected from light; equilibrate to room temperature 20 min before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2-8 °C (corrosive; handle with PPE)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (sandwich double-antibody)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2–8 °C. Check individual component labels and recommended storage after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit reagents to room temperature for 20 minutes before use; return to 2–8 °C immediately after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles of samples; for long-term storage use ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra-pure water; water temperature should not exceed 37 °C when preparing buffers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) — it will inactivate HRP and cause underestimation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Sorbitol may cause high background; sample sorbitol concentration should not exceed 0.01%.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5 to avoid assay interference.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Biotinylated antibody and Streptavidin-HRP dilutions fresh for each assay; recommended for single use due to stability.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive: avoid contact with eyes, skin, and clothing; wear goggles and appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after termination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402421.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402421.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitative measurement of nonspecific endonuclease (SMNE) in bioprocess samples (in-process material, harvested bulk, drug substance and drug product). It is for research use only and not for clinical diagnostics.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and working range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLinear range 0.1–25 ng\/mL with a reported LLOQ of 0.25 ng\/mL; a 4-parameter logistic (4-PL) regression is used for quantitation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit detect Benzonase® or other engineered Serratia marcescens endonucleases?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — the kit is designed to detect genetically engineered endonucleases from Serratia marcescens, such as Benzonase®.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store opened components and how long are they stable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the kit at 2–8 °C. Calibration standard is stable at 2–8 °C for up to 12 months (check tube label); opened microtiter strips stored with desiccant at 2–8 °C are stable up to 30 days. Other reagents should be used according to label and prepared fresh where indicated.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO); not intended for clinical use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189209161909,"sku":"1402421","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/NonspecificEndonuclease_SMNE_ELISAKit.jpg?v=1742294517"},{"product_id":"human-serum-albumin-hsa-elisa-kit-one-step-elisa-shentek®","title":"Human Serum Albumin (HSA) ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eLess than 3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (opened strips stable up to 90 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eOne-step double-antibody sandwich ELISA for quantitative detection of Human Serum Albumin (HSA). The kit uses pre-coated microtiter strips with polyclonal anti-HSA capture antibody. Calibration standards (or samples) and HRP-conjugated anti-HSA detection antibody are added simultaneously (one-step format), incubated, washed, then developed with TMB substrate; HRP catalyzes TMB oxidation to generate a colorimetric signal read at 450 nm (with long-wavelength correction 620–650 nm). The assay is designed for sensitive, specific quantitation of trace HSA in biological products (natural and recombinant) and is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat \/ Scale\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit (8 × 12 well strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay type\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step sandwich ELISA (simultaneous addition of sample and HRP-conjugated detection antibody)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinear range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.25–16 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity (fit)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eR² ≥ 0.990 (typical calibration R² = 0.99998)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.25 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration model \/ equation\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL): y = (A - D) \/ [1 + (x\/C)^B] + D; example parameters A=5.91069, B=-1.06512, C=20.56331, D=0.00881, R²=0.99998\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity reported with fetal bovine serum (FBS) and horse serum\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product; recommended validation for other biological matrices\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.0–8.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReading wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference\/correction 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eSample reaction: ~2 hours at room temperature on shaker (500–600 rpm); TMB development: 10 min at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step assay format:\u003c\/strong\u003e Simultaneous addition of sample and HRP-conjugated detection antibody simplifies workflow and reduces hands-on time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ of 0.25 ng\/mL and linear range down to 0.25 ng\/mL for trace-level HSA detection.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSpecific double-antibody sandwich:\u003c\/strong\u003e Polyclonal anti-HSA capture antibody with HRP-conjugated detection antibody provides high specificity for HSA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use pre-coated strips:\u003c\/strong\u003e Strips are pre-coated and vacuum-sealed with desiccant for convenience and reduced lot-to-lot handling.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQuantitative output:\u003c\/strong\u003e 4-parameter logistic (4PL) calibration curve for accurate quantitation across the stated range.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of Human Serum Albumin (HSA) in in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eMeasurement of HSA in harvested bulk (bioprocess samples)\u003c\/li\u003e\r\n                    \u003cli\u003eQuantitation of HSA in drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eDetection of natural and recombinant HSA in research workflows\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and process monitoring in biomanufacturing research\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHSA Calibration Standard (PNB018)\u003c\/span\u003e\u003cstrong\u003e1 × 150 μL — refer to tube label; store at 2-8 °C; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-HSA Microtiter Strips (PNA017)\u003c\/span\u003e\u003cstrong\u003e8 strips × 12 wells — pre-coated; vacuum bag with desiccant; store at 2-8 °C (unused strips: keep sealed with desiccant)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL — store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e1 × 25 mL — store at 2-8 °C; may crystallize at low temp (incubate at 37 °C to dissolve)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-HSA:HRP (100×) (PNN012)\u003c\/span\u003e\u003cstrong\u003e1 × 120 μL — store at 2-8 °C; dilute 100× in Diluent immediately before use; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL — sealed, protect from light, equilibrate to room temp before use; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002, 1 M HCl)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL — corrosive (1 M HCl); store at 2-8 °C; handle with goggles and appropriate PPE\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces — room temperature (cover plate during incubation to prevent evaporation)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step sandwich ELISA (double-antibody, HRP\/TMB colorimetric)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store entire kit at 2–8 °C; check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Opened microtiter strips: store sealed with desiccant at 2–8 °C; stable up to 90 days.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Anti-HSA:HRP (1×) freshly by 100× dilution in Diluent immediately before use; avoid repeated freeze-thaw.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB substrate must be colorless before use; discard if colored. Protect from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive. Avoid contact with skin, eyes, and clothing; use appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid sodium azide (NaN3) in samples or reagents — it will deactivate HRP and cause underestimation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended sample pH between 6.0 and 8.0; outside this range may cause abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition and avoid excessive foaming during dilutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different kit lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile, disposable tips and reservoirs. Minimum recommended pipetting volume: 5 μL.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after termination.\u003c\/p\u003e\r\n                    \u003cp\u003e• If Wash Buffer Concentrate or Diluent is cloudy or contains precipitate, heat at 37 °C until clear.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402427.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402427.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay range and sensitivity (LLOQ) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.25–16 ng\/mL with a reported LLOQ of 0.25 ng\/mL. Typical calibration shows R² ≥ 0.990 (example R² = 0.99998).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sample types can be tested with this kit and are validations required?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIntended for in-process samples, harvested bulk, drug substance and drug product. For other biological matrices, a method validation is recommended to verify sample suitability and recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How long does the assay take to run?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTypical workflow requires a ~2 hour sample incubation at room temperature plus ~10 minutes for TMB development and additional wash\/handling steps; overall time is less than 3 hours.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should critical reagents be stored and handled?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit at 2–8 °C. Prepare Anti-HSA:HRP fresh by 100× dilution in Diluent before each assay. Keep TMB protected from light and ensure it is colorless before use. Keep unused strips sealed with desiccant.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not intended for clinical or diagnostic use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189216010421,"sku":"1402427","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Serum_Albumin_HSA_ELISA_Kit_One-step_ELISA.jpg?v=1742295025"},{"product_id":"deoxyribonuclease-i-dnase-elisa-kit-shentek®","title":"Deoxyribonuclease I (DNase) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (opened strips stable for 90 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK Deoxyribonuclease I (DNase I) ELISA Kit is a 96-well, sandwich-format immunoassay for quantitative measurement of DNase I impurities in bioprocess samples and finished products (e.g., mRNA vaccines and therapeutics). The assay uses sheep polyclonal anti-DNase I capture antibody coated on microtiter strips, a biotinylated anti-DNase I detection antibody, and streptavidin-HRP for signal generation. TMB substrate is oxidized by HRP and measured at 450 nm (with 620–650 nm reference); concentration is determined using a 4-parameter logistic (4-PL) calibration curve. The kit is intended for Research Use Only (RUO) and supports testing from in-process samples through drug substance and drug product.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay principle\u003c\/td\u003e\r\n                        \u003ctd\u003eDouble-antibody sandwich ELISA (capture antibody coated strips; biotinylated detection antibody; streptavidin-HRP; TMB substrate)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.5–32 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration curve model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical 4-PL parameters (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eA = 2.50521; B = -0.70719; C = 20.59525; D = -0.04360; R² = 0.99946\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMeasurement wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation times (per protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 1 hour (RT, 400–600 rpm); Biotinylated antibody: 45 min (RT); Streptavidin-HRP: 30 min (RT); TMB reaction: 15 min (RT); Post-stop incubation: 5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with T7 RNA Polymerase, RNase Inhibitor, BsaI; no cross-reactivity with host cell proteins from P. pastoris, MDCK, Vero, HEK293, Sf9, CHO, E. coli\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample matrix \/ intended samples\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance and drug product (e.g., mRNA production workflows)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptable sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003e5 µL (recommended minimum for dilutions)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd\u003eStore kit at 2–8 °C; protect light-sensitive components (TMB, streptavidin-HRP, biotinylated conjugate)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity and defined quantitation range:\u003c\/strong\u003e LLOQ 0.5 ng\/mL with linear range 0.5–32 ng\/mL and high curve fit (R² ≈ 0.999)\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity with key enzymes used in mRNA production (T7 RNA Polymerase, RNase inhibitor, BsaI) or common host cell proteins\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified sheep anti-DNase I capture antibody for consistent plate-to-plate performance\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete reagent set for ELISA:\u003c\/strong\u003e Includes calibration standard, diluent, wash buffer concentrate, biotinylated detection antibody, streptavidin-HRP, TMB substrate, and stop solution\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for bioprocess monitoring:\u003c\/strong\u003e Suitable for quantitation of DNase I from in-process samples to final drug product, including mRNA workflows\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of DNase I impurities during mRNA vaccine and therapeutic production\u003c\/li\u003e\r\n                    \u003cli\u003eTesting in-process samples, harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and release testing (research use)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and spike-recovery studies for DNase I monitoring\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNase I Calibration Standard (PNB016)\u003c\/span\u003e\u003cstrong\u003e1 × 350 µL; store at 2–8 °C; refer to tube label for details\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-DNase I Microtiter Strips (PNA016)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated and packaged with desiccant; store in sealed bag at 2–8 °C (opened strips stable up to 90 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE008)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C; dilute 1:10 with ultrapure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-DNase I: Biotinylated Conjugate (100×) (PNG008)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; sealed, protect from light; store at 2–8 °C; dilute 1:100 with Diluent fresh before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002)\u003c\/span\u003e\u003cstrong\u003e1 × 140 µL; sealed, protect from light; store at 2–8 °C; dilute 1:100 with Diluent fresh before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, protect from light; equilibrate to room temp 20 min before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL (1 M HCl); store at 2–8 °C; handle with goggles and protective equipment\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubations; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit and components at 2–8 °C. Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Handle Stop Solution (1 M HCl) with care; avoid contact with eyes\/skin; wear goggles and protective clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents (TMB, streptavidin-HRP, biotinylated conjugate) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples; for long-term storage keep samples at ≤ −70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples should have pH between 6.5 and 8.0; values outside this range may cause abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultrapure water (temperature below 37 °C) for reagent preparation; if diluent or wash buffer has precipitate, heat to 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume is 5 µL for dilution accuracy; avoid bubbles and foaming when pipetting.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge concentrated conjugates (100×) before use and prepare working solutions fresh; single-use recommended for some prepared solutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use samples containing sodium azide (NaN3) as it will inactivate HRP and underestimate DNase I levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402428.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402428.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.5–32 ng\/mL with an LLOQ of 0.5 ng\/mL. Typical calibration fits a 4-parameter logistic model (example R² ≈ 0.999).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should samples be prepared and stored prior to testing?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSamples must be clear\/transparent; remove insoluble material by centrifugation or filtration. Avoid repeated freeze–thaw cycles; for long-term storage store at ≤ −70 °C. Adjust sample dilution to fit the calibration curve and ensure pH between 6.5–8.0.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this assay cross-react with enzymes used in mRNA production or common host cell proteins?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The kit has been tested and shows no cross-reactivity with T7 RNA Polymerase, RNase Inhibitor, BsaI, or host cell proteins from P. pastoris, MDCK, Vero, HEK293, Sf9, CHO, and E. coli.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe high background signal?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include reagent or equipment contamination, insufficient washing, or environmental contamination. Use freshly prepared reagents, increase wash volume\/times, clean equipment with 75% ethanol, and avoid cross-contamination of wells.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189219909813,"sku":"1402428","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DeoxyribonucleaseI_DNase_ELISAKit.jpg?v=1742295412"},{"product_id":"dsdna-hs-assay-kit","title":"dsDNA HS Assay Kit","description":"\u003cstyle\u003e\n  .bf-rich .josh-faq {\n    background: #fff;\n    color: #000;\n    font-family: \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n\n  .bf-rich .josh-faq__question {\n    width: 100%;\n    margin: 0;\n    padding: 20px 0;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    color: #10213d;\n    background: none;\n    border: 0;\n    font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif;\n    text-align: left;\n    cursor: pointer;\n  }\n\n  .bf-rich .josh-faq__question:hover { color: #055da9; }\n\n  .bf-rich .josh-faq__icon {\n    position: relative;\n    width: 20px;\n    height: 20px;\n    flex: 0 0 20px;\n    transition: transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before,\n  .bf-rich .josh-faq__icon::after {\n    position: absolute;\n    content: \"\";\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity .25s ease, transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before {\n    top: 50%;\n    left: 0;\n    width: 100%;\n    height: 2px;\n    transform: translateY(-50%);\n  }\n\n  .bf-rich .josh-faq__icon::after {\n    top: 0;\n    left: 50%;\n    width: 2px;\n    height: 100%;\n    transform: translateX(-50%);\n  }\n\n  .bf-rich .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .bf-rich .josh-faq__answer {\n    display: none;\n    max-width: 860px;\n    padding: 0 0 20px;\n    color: #333;\n    font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__answer.is-open { display: block; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #333; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n\n  @media (max-width: 767px) {\n    .bf-rich .josh-faq__inner { padding: 24px 0 40px; }\n    .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; }\n    .bf-rich .josh-faq__answer { font-size: 15px; }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLow-concentration double-stranded DNA can be difficult to quantify by UV A260 measurement. The Biofargo dsDNA HS Assay Kit uses fluorescence-based detection to quantify dsDNA with high selectivity over RNA. Its total assay range is 0.1–120 ng, using 1–20 µL of sample in a 200 µL reaction.\u003c\/p\u003e\n\u003cp\u003eThe kit includes fluorescent reagent, assay buffer and two dsDNA standards. Samples can be measured individually with a Qubit fluorometer or in a black 96-well microplate with a fluorescence microplate reader. After a 2-minute incubation at room temperature, protected from light, the signal remains stable for up to 3 hours when protected from light. The kit is available in 20- and 500-reaction formats for research use only.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo dsDNA HS Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDetection method\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorescence-based quantification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eInitial sample concentration range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e5 pg\/µL–120 ng\/µL, depending on sample volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal assay range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.1–120 ng dsDNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1–20 µL in a final assay volume of 200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMeasurement formats\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMicroplate reader settings\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e485 nm excitation; 530 nm emission\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStandards\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eQS0: 0 ng\/µL; QS1: 10 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 minutes at room temperature, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAvailable formats\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFS-T1001-S: 20 reactions; FS-T1002: 500 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2–8°C upon arrival; protect fluorescent reagents from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity dsDNA quantification: Measures 0.1–120 ng of dsDNA per assay.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSelective fluorescence detection: Provides high selectivity for dsDNA over RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLow sample requirement: Uses 1–20 µL of sample in a 200 µL reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTwo measurement formats: Supports individual measurements with a Qubit fluorometer or 96-well measurements with a fluorescence microplate reader.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eShort incubation: Requires 2 minutes at room temperature while protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow-Concentration dsDNA Quantification:\u003c\/strong\u003e For fluorescence-based measurement of dsDNA samples within the stated assay range.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIndividual Sample Measurement:\u003c\/strong\u003e For dsDNA quantification using a Qubit fluorometer and thin-walled, clear 0.5 mL analysis tubes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e96-Well Plate Measurement:\u003c\/strong\u003e For dsDNA quantification using a fluorescence microplate reader and black-wall, clear-bottom 96-well microplates.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1001-S (20 reactions)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1002 (500 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Buffer QB\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Standard QS0 (0 ng\/µL)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Standard QS1 (10 ng\/µL)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA HS Fluorescent Reagent QF\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eShip on ice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore at 2–8°C upon arrival.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtect fluorescent reagents from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cdiv class=\"josh-faq\"\u003e\n\u003csection class=\"josh-faq__inner\"\u003e\n\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e What does the Biofargo dsDNA HS Assay Kit measure? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eIt measures double-stranded DNA by fluorescence and provides high selectivity for dsDNA over RNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e What is the assay range? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eThe total assay range is 0.1–120 ng of dsDNA. The supported initial sample concentration is 5 pg\/µL–120 ng\/µL, depending on sample volume.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e How much sample is needed? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eEach assay uses 1–20 µL of sample in a final reaction volume of 200 µL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e Can the kit be used with a fluorescence microplate reader? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The protocol specifies black-wall, clear-bottom 96-well microplates and reader settings of 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e What sizes are available and how should the kit be stored? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eFS-T1001-S provides 20 reactions and FS-T1002 provides 500 reactions. Store the kit at 2–8°C upon arrival and protect fluorescent reagents from light.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dsDNA_HS_Assay_Kit-User_guide.pdf?v=1789695414\" target=\"_blank\"\u003e\u003cstrong\u003eProduct User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Qihengxing","offers":[{"title":"20 Reactions","offer_id":53189979177141,"sku":"FS-T1001-S","price":39.0,"currency_code":"USD","in_stock":true},{"title":"500 reactions","offer_id":53189979209909,"sku":"FS-T1002","price":299.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-ad012ae6-7232-4929-b4b0-121a0bdbda05.png?v=1788164333"},{"product_id":"dsdna-br-assay-kit","title":"dsDNA BR Assay Kit","description":"\u003cstyle\u003e\n  .bf-rich .josh-faq {\n    background: #fff;\n    color: #000;\n    font-family: \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__inner { padding: 30px 300px 60px 30px; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n\n  .bf-rich .josh-faq__question {\n    width: 100%;\n    margin: 0;\n    padding: 20px 0;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    color: #10213d;\n    background: none;\n    border: 0;\n    font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif;\n    text-align: left;\n    cursor: pointer;\n  }\n\n  .bf-rich .josh-faq__question:hover { color: #055da9; }\n\n  .bf-rich .josh-faq__icon {\n    position: relative;\n    width: 20px;\n    height: 20px;\n    flex: 0 0 20px;\n    transition: transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before,\n  .bf-rich .josh-faq__icon::after {\n    position: absolute;\n    content: \"\";\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity .25s ease, transform .25s ease;\n  }\n\n  .bf-rich .josh-faq__icon::before {\n    top: 50%;\n    left: 0;\n    width: 100%;\n    height: 2px;\n    transform: translateY(-50%);\n  }\n\n  .bf-rich .josh-faq__icon::after {\n    top: 0;\n    left: 50%;\n    width: 2px;\n    height: 100%;\n    transform: translateX(-50%);\n  }\n\n  .bf-rich .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .bf-rich .josh-faq__answer {\n    display: none;\n    max-width: 860px;\n    padding: 0 0 20px;\n    color: #333;\n    font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif;\n  }\n\n  .bf-rich .josh-faq__answer.is-open { display: block; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #333; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n\n  @media (max-width: 767px) {\n    .bf-rich .josh-faq__inner { padding: 24px 0 40px; }\n    .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; }\n    .bf-rich .josh-faq__answer { font-size: 15px; }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eLaboratories measuring double-stranded DNA by UV absorbance at 260 nm may encounter interference from RNA, proteins, salts, and detergents. The Biofargo dsDNA BR Assay Kit uses a fluorescence-based method that is highly selective for dsDNA over RNA and is minimally affected by the common contaminants evaluated in the user guide.\u003c\/p\u003e\n\u003cp\u003eDepending on sample volume, the assay measures initial dsDNA concentrations from 0.2 to 1,000 ng\/µL, with a total assay range of 2–1,000 ng. Each assay uses 1–20 µL of sample in a final volume of 200 µL. The protocol supports a Qubit fluorometer or a fluorescence microplate reader.\u003c\/p\u003e\n\u003cp\u003eThe assay requires a 2-minute incubation at room temperature protected from light. The signal remains stable for up to 3 hours after incubation when protected from light. For research use only; not for diagnostic or therapeutic procedures.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo dsDNA BR Assay Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTarget\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDouble-stranded DNA (dsDNA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDetection method\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eInitial dsDNA concentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.2–1,000 ng\/µL, depending on sample volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal assay range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2–1,000 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1–20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eFinal assay volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e200 µL per tube or well\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay temperature\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e20–25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 minutes, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eQubit fluorometer or fluorescence microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMicroplate reader settings\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e485 nm excitation; 530 nm emission\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAvailable formats\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFS-T1004-S: 100 reactions; FS-T1004: 1,000 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eResearch use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBroad-range dsDNA measurement with a total assay range of 2–1,000 ng.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHighly selective for double-stranded DNA over RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAccepts 1–20 µL of sample in a final assay volume of 200 µL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtocols for a Qubit fluorometer and a fluorescence microplate reader.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eA 2-minute incubation at room temperature, protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFluorescent signal remains stable for up to 3 hours after incubation when protected from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQubit Fluorometer:\u003c\/strong\u003e Quantify dsDNA in clear, thin-wall 0.5 mL PCR tubes using the two supplied standards and the dsDNA Broad Range assay setting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Microplate Reader:\u003c\/strong\u003e Quantify dsDNA in a black-wall, clear-bottom 96-well microplate using a standard curve, 485 nm excitation, and 530 nm emission.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1004-S (100 reactions)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFS-T1004 (1,000 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA BR Buffer QB\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA BR Standard QS0\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL, 0 ng\/µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL, 0 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA BR Standard QS1\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL, 100 ng\/µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL, 100 ng\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003edsDNA BR Fluorescent Reagent QF\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 µL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eShip on ice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStore at 2–8 °C upon arrival.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtect the fluorescent dye and working solution from light.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare the working solution fresh for each assay.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cdiv class=\"josh-faq\"\u003e\n\u003csection class=\"josh-faq__inner\"\u003e\n\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e What is the detection range of the Biofargo dsDNA BR Assay Kit? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eThe total assay range is 2–1,000 ng. Depending on sample volume, the initial dsDNA concentration range is 0.2–1,000 ng\/µL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e Can the kit be used with a Qubit fluorometer? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The user guide includes a Qubit workflow using two standards. On a Qubit 4 Fluorometer, select dsDNA and then dsDNA Broad Range.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e Can the kit be used with a fluorescence microplate reader? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eYes. The user guide includes a 96-well workflow with 485 nm excitation and 530 nm emission.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e How much sample is needed? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eAdd 1–20 µL of sample and adjust the working solution so that the final volume is 200 µL per tube or well.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"josh-faq__item\"\u003e\n\u003cbutton type=\"button\" class=\"josh-faq__question\"\u003e How should the kit be stored? \u003cspan class=\"josh-faq__icon\"\u003e\u003c\/span\u003e \u003c\/button\u003e\n\u003cdiv class=\"josh-faq__answer\"\u003e\n\u003cp\u003eThe kit is shipped on ice and should be stored at 2–8 °C upon arrival. Protect the fluorescent dye and freshly prepared working solution from light.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dsDNA_BR_Assay_Kit-User_guide.pdf?v=1789695414\" target=\"_blank\"\u003e\u003cstrong\u003eProduct User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Qihengxing","offers":[{"title":"100 Reactions","offer_id":53189995823285,"sku":"FS-T1004S","price":79.0,"currency_code":"USD","in_stock":true},{"title":"1000 Reactions","offer_id":53189995856053,"sku":"FS-T1004","price":399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dsdna-br-assay-kit-white-background-1500.jpg?v=1789626240"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/dsDNA.png?v=1768801367","url":"https:\/\/biofargo.com\/collections\/qpcr-quantitation-kits.oembed?page=4","provider":"Biofargo","version":"1.0","type":"link"}