{"title":"Protein Extraction \u0026 Lysis","description":"","products":[{"product_id":"ripa-lysis-buffer-i","title":"RIPA Lysis Buffer I","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 114px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003e10 PREPS\/50 ML\/100 ML\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRIPA Lysis Buffer I\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSuitable for the detection of WB, IP (immune co precipitation), and most antigen epitopes.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eRIPA Lysis Buffer I has a strong lysis effect on the membrane, cytoplasm, and nuclear components of animal cells, and may be a classic but commonly used cell tissue lysis fluid. The use of RIPA Lysis Buffer to prepare lysis products for Western Blot and immunoprecipitation is already a standard operation, suitable for the detection of most antigenic epitopes, especially for immunoprecipitation detection of protein-protein interactions, cytoplasmic phosphorylated proteins, and nuclear transcription factor detection. This product is composed of lysate, protease, and phosphatase inhibitor (P310118-0100 only includes lysate), and can process 107 cells or 100 mg of tissue at a time.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eStrong lysis effect, good extraction effect for nuclear protein and membrane protein.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSolution A, Solution B, Solution C (P310118-0100 does not include Solutions B and C).\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19px; text-align: center;\"\u003e\u003cstrong\u003eSolution A, Solution B, Solution C (P310118-0100 does not include Solutions B and C).\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore separately according to label prompts\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"10PREPS","offer_id":52400979706037,"sku":"P310118-10PREPS","price":31.0,"currency_code":"USD","in_stock":true},{"title":"50ml","offer_id":52400979738805,"sku":"P310118-50ml","price":131.0,"currency_code":"USD","in_stock":true},{"title":"100ml","offer_id":52400979771573,"sku":"P310118-100ml","price":45.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_lysisbuffer.avif?v=1751472360"},{"product_id":"tissue-or-cell-total-protein-extraction","title":"Tissue or Cell Total Protein Extraction","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"desc-box\"\u003e\n      \u003cp style=\"margin:0;\"\u003eThis kit can extract whole proteins from mammalian tissues and cultured cells. Lysis Buffer homogenate containing a mixture of protease inhibitors and phosphatase inhibitors is used to lyse tissues or cells, with a mild effect and a simple and efficient extraction process. It can extract the vast majority of proteins from cultured cells or animal tissues. Proteins can be extracted from 107 cultured cells or 200 mg of animal tissue at a time.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specification --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table w-640\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n            \u003cth\u003eSpecification\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003e50 ASSAYS\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eTissue or Cell Total Protein Extraction\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComposition\u003c\/td\u003e\n            \u003ctd\u003eLysis buffer, Protease inhibitors, Phosphatase inhibitors, PMSF\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComponent\u003c\/td\u003e\n            \u003ctd\u003eLysis buffer, Protease inhibitors, Phosphatase inhibitors, PMSF\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eFeatures\u003c\/h2\u003e\n    \u003cul\u003e\n      \u003cli\u003eThe extracted proteins include membrane, nucleus, nuclear matrix, and cytoplasmic proteins.\u003c\/li\u003e\n      \u003cli\u003eThe entire operation process only takes about 30 minutes.\u003c\/li\u003e\n      \u003cli\u003eIt can be used to culture cells (50 × 10 ^ 7), can also be used in animal tissues (50 × 200 mg) protein extraction.\u003c\/li\u003e\n      \u003cli\u003eAvoid protein degradation by proteases and phosphatases, maintain protein integrity and natural activity.\u003c\/li\u003e\n      \u003cli\u003eNo need for high-speed centrifugation, multiple samples can be processed simultaneously.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eThe extracted whole protein can be used for Western Blot, immunoprecipitation, enzyme activity analysis, Pull down, EMSA and other subsequent studies, but cannot be used for protein kinase and phosphatase immunoprecipitation studies (inhibitors of these two enzymes are included in this kit).\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eStore separately according to label prompts\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"50ASSAYS","offer_id":52400979673269,"sku":"P310119-50ASSAYS","price":111.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5fc46855-9b9e-40a9-ae38-e4530212e657.jpg?v=1773021011"},{"product_id":"bacterial-protein-extraction-kit","title":"Bacterial Protein Extraction Kit","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  \n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"desc-box\"\u003e\n      \u003cp style=\"margin:0;\"\u003eThe bacterial total protein extraction kit uses mild ionic detergent for lysis and extraction, without the need for ultrasound or repeated freeze-thaw; Add compatible protease inhibitors to ensure protein integrity as much as possible; The extracted proteins can be used for affinity purification of subsequent FLAG, poly Hiss, and GST labeled proteins, and can also be compatible with many protein biochemical assays.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specification --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table w-640\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n            \u003cth\u003eSpecification\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003e10 ASSAYS\/50 ASSAYS\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eBacterial Protein Extraction Kit\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComposition\u003c\/td\u003e\n            \u003ctd\u003e10X Cell lysis buffer, DNase\/Rnase, Lysozyme, PMSF, 2X SDS gel-loading buffer\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComponent\u003c\/td\u003e\n            \u003ctd\u003e10X Cell lysis buffer, DNase\/Rnase, Lysozyme, PMSF, 2X SDS gel-loading buffer\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eFeatures\u003c\/h2\u003e\n    \u003cul\u003e\n      \u003cli\u003eEasy and fast operation.\u003c\/li\u003e\n      \u003cli\u003eNo special instruments and equipment are required.\u003c\/li\u003e\n      \u003cli\u003eCan be stored for a long time.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eIt can be used to extract total protein in 1-5 L bacterial culture medium, and two different packaged kits can provide 10 and 50 reactions, respectively.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eStore separately according to label prompts\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ASSAYS","offer_id":52400979607733,"sku":"P310120-10ASSAYS","price":56.0,"currency_code":"USD","in_stock":true},{"title":"50ASSAYS","offer_id":52400979640501,"sku":"P310120-50ASSAYS","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_7c0738df-d3d8-427e-9eca-2c6f74bbc8af.jpg?v=1773020736"},{"product_id":"micro-bca-protein-assay-kit","title":"Micro BCA Protein Assay Kit","description":"\u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 587.813px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003ePackage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003e1250 ASSAYS\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eMicro BCA Protein Assay Kit\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eUsed for protein quantitative testing\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 254.719px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 254.719px; text-align: center;\"\u003e\u003cstrong\u003eSummary\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 254.719px; text-align: center;\"\u003e\u003cstrong\u003eThe BCA method is an ideal protein quantification method, which combines proteins with Cu2+in alkaline environments to reduce Cu2+to Cu1+. BCA combines with Cu1+to form a stable purple blue complex, which has a maximum light absorption value at 562 nm and is proportional to protein concentration. Based on this, protein concentration can be determined. This determination method has high sensitivity, simple operation, good stability of the reagent and its formed color complex, and is less affected by interfering substances such as detergents. This reagent kit is suitable for the determination of trace protein concentration.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 137.156px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 137.156px; text-align: center;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 137.156px; text-align: center;\"\u003e\u003cstrong\u003eSuitable for quantification of low concentration protein samples; Quantitative range: spectrophotometry 0.5-20 μ G\/ml; Enzyme labeling plate method 2-40 μg\/ml; Compatible with ionic or non ionic decontamination reagents; The coefficient of variation for detecting different protein molecules is much smaller than the Coomassie brilliant blue method.\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 39.1875px; text-align: center;\"\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px; text-align: center;\"\u003e\u003cstrong\u003eReagent A, Reagent B, Reagent C, BSA standard solution (5 mg\/ml)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eAssay\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eBCA Assay\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eDetection Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eColorimetric\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eFor Use With (Equipment)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 19.5938px; text-align: center;\"\u003e\u003cstrong\u003eSpectrophotometer, Microplate Reader\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 39.1875px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 39.1875px; text-align: center;\"\u003e\u003cstrong\u003eReagent A, Reagent B, Reagent C, BSA standard solution (5 mg\/ml)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore separately according to label prompts\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"KleanAB","offers":[{"title":"1250ASSAYS","offer_id":52400979574965,"sku":"P310125-1250","price":70.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/d1fe94ae-be8f-4c9d-bec2-d71141fb88bf_1.jpg?v=1738720482"},{"product_id":"np-40-biofargo","title":"NP-40- Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eNP-40\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e:1\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e:RT\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e:12161902\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e:Detergents\/Surfactants\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCAS\u003c\/strong\u003e:[9016-45-9]\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e:Reagent\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDG:NoStorage\u003c\/strong\u003e:18 to 25C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSterile\u003c\/strong\u003e:No\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStock\u003c\/strong\u003e:Usually in Stock\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA_NDB0385-NO_LOT.pdf?v=1733983177\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.NDB0385-NP-40_octyl_phenoxypolyethoxylethanol.pdf?v=1733983191\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"100mL","offer_id":44905190817973,"sku":"NDB0385-100mL","price":29.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":44905190850741,"sku":"NDB0385-500mL","price":113.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/web-250g-bottle-v1_381_1.jpg?v=1733982901"},{"product_id":"triton-x-100-biofargo","title":"Triton X-100","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003carticle data-scroll-anchor=\"true\" data-testid=\"conversation-turn-34\" dir=\"auto\" class=\"group\/turn w-full text-token-text-primary focus-visible:outline-2 focus-visible:outline-offset-[-4px]\"\u003e\n\u003cdiv class=\"text-base my-auto mx-auto py-[18px] px-6\"\u003e\n\u003cdiv class=\"mx-auto flex flex-1 text-base gap-4 md:gap-5 lg:gap-6 md:max-w-3xl lg:max-w-[40rem] xl:max-w-[48rem]\"\u003e\n\u003cdiv class=\"group\/conversation-turn relative flex w-full min-w-0 flex-col agent-turn @xs\/thread:px-0 @sm\/thread:px-1.5 @md\/thread:px-4\"\u003e\n\u003cdiv class=\"flex-col gap-1 md:gap-3\"\u003e\n\u003cdiv class=\"flex max-w-full flex-col flex-grow\"\u003e\n\u003cdiv data-message-model-slug=\"gpt-4o\" class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 whitespace-normal break-words text-start [.text-message+\u0026amp;]:mt-5\" dir=\"auto\" data-message-id=\"79b5aedf-812c-4afe-a564-5687cb92e76c\" data-message-author-role=\"assistant\"\u003e\n\u003cdiv class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[3px]\"\u003e\n\u003cdiv class=\"markdown prose w-full break-words dark:prose-invert light\"\u003e\n\u003cp data-is-only-node=\"\" data-is-last-node=\"\" data-end=\"606\" data-start=\"92\"\u003eTriton X-100, also known as PEG T-Octylphenyl Ether (N = 9-10), is a high-quality non-ionic surfactant widely used in laboratory and research settings. With a molecular weight of 647 g\/mol, it is an essential reagent for cell lysis, protein extraction, and membrane permeabilization. Its gentle yet effective detergent properties make it ideal for solubilizing membrane proteins, enabling downstream applications such as immunostaining, protein purification, and lipid raft studies.\u003c\/p\u003e\n\u003ch2 data-end=\"606\" data-start=\"92\"\u003eSpecification\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/article\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTriton X-100S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e647 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eQuantity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 ml (1 container)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRoom Temperature (RT)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e12161902\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDetergents\/Surfactants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHazard Class\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eClass 9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUN Number\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUN382\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePacking Group\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePGIII\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCAS Number\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e[9002-93-1]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDangerous Goods\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e18–25°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAvailability\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUsually in stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong data-end=\"505\" data-start=\"488\"\u003eApplications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-end=\"1224\" data-start=\"508\"\u003e\n\u003cli data-end=\"654\" data-start=\"508\"\u003e\n\u003cstrong data-end=\"548\" data-start=\"510\"\u003eCell Lysis and Protein Extraction:\u003c\/strong\u003e Efficiently disrupts cell membranes, enabling the release and solubilization of intracellular proteins.\u003c\/li\u003e\n\u003cli data-end=\"817\" data-start=\"655\"\u003e\n\u003cstrong data-end=\"687\" data-start=\"657\"\u003eMembrane Permeabilization:\u003c\/strong\u003e Used to gently permeabilize cell membranes, facilitating antibody penetration for immunostaining or other intracellular assays.\u003c\/li\u003e\n\u003cli data-end=\"955\" data-start=\"818\"\u003e\n\u003cstrong data-end=\"838\" data-start=\"820\"\u003eAssay Buffers:\u003c\/strong\u003e Commonly included in buffer systems to stabilize proteins, reduce background noise, and improve assay sensitivity.\u003c\/li\u003e\n\u003cli data-end=\"1093\" data-start=\"956\"\u003e\n\u003cstrong data-end=\"981\" data-start=\"958\"\u003eLipid Raft Studies:\u003c\/strong\u003e Aids in isolating lipid rafts from cell membranes for studying signal transduction and membrane organization.\u003c\/li\u003e\n\u003cli data-end=\"1224\" data-start=\"1094\"\u003e\n\u003cstrong data-end=\"1121\" data-start=\"1096\"\u003eProtein Purification:\u003c\/strong\u003e Helps solubilize membrane proteins and maintain their functional activity for downstream applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.TB0198_A600198_-_NO_LOT_-V3.pdf?v=1733984438\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.TB0198-Triton_X-100.pdf?v=1733984451\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eFor laboratory research use only.\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44905211396277,"sku":"TB0198-500mL","price":24.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TritonX-100.jpg?v=1747470041"},{"product_id":"triton-x-114-biofargo","title":"Triton X-114- Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTriton X-114\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Product Size\u003c\/strong\u003e:500ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIndividual Container Size\u003c\/strong\u003e:500ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e:1\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e:RT\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e:12161902\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e:Detergents\/Surfactants\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCAS\u003c\/strong\u003e:[9036-19-5]\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e:Biotech\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDG:\u003c\/strong\u003eNo\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e:18 to 25C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSterile\u003c\/strong\u003e:NoStock:Usually in Stock\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.TDB0554_A620554_-NO_LOT_-V3.pdf?v=1733984989\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.TDB0554-Triton_X-114.pdf?v=1733985005\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44905219293365,"sku":"TDB0554-500mL","price":46.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/500ml_bottle_new_v2_sm_w_29.png?v=1733984860"},{"product_id":"bsa-standard-solution-biofargo","title":"BSA Standard Solution","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBBI BSA Standards are high-quality reference samples for generating accurate standard curves and calibration controls in total protein assays.\u003c\/p\u003e\n\u003cp\u003eThe bovine serum albumin (BSA) solution is protein concentration reference standards for use in BCA, Bradford and other protein assay protocols. BSA is the universally accepted reference protein for total protein quantitation. The albumin standard is precisely formulated at 20mg\/mL in saline buffer. Rotease is not detected.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHigh Purity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAppearance\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTotal BSA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e19.0-21.0 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContain Sodium Azide\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2-8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eTotal BSA: 19.0-21.0mg\/ml\u003c\/p\u003e\n\u003cp\u003eSodium azide: 0.1%\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eProtein assay quantitation standard (BCA Protein Assay, Coomassie-Bradford Assay, etc.) \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eProtein recovery control for desalting and other column procedures \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eLoading control for SDS-PAGE \u003c\/li\u003e\n\u003cli\u003eGeneral calibration of spectrophotometer UV-lamp (absorbance at 280nm)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/info.AJ642-v5.pdf?v=1734143725\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eINFO\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.AJ642-NO_LOT.pdf?v=1734143784\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOC\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.AJ642-BSA_Standard_Solution_Bovine_Serum_Albumin.pdf?v=1734143788\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"1mL","offer_id":44909312278709,"sku":"AJ642-1mL","price":17.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BSAStandardSolution_6.jpg?v=1734512092"},{"product_id":"5xripa-buffer-iv-with-triton-x-100-ph-7-4-5xconcentrate-biofargo","title":"5xRIPA Buffer IV with Triton-X-100 (pH 7.4) 5xconcentrate","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e5 x RIPA Buffer IV with Triton-X-100 (pH 7.4) 5x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and\/or interference with protein immunoreactivity and biological activity. RIOA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of the this buffer. In addition, RIPA buffer minimizes non-specific protein-binding most specific interactions to occur, enabling studies of relevant protein-protein interactions.RIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X107 cells) of most adherent mammalian cell lines\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Product Size\u003c\/strong\u003e:500ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIndividual Container Size\u003c\/strong\u003e:500ml\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e:1\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRefrigeration Requirements\u003c\/strong\u003e:Refrigerator\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Conditions:\u003c\/strong\u003eICE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e:12161700\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e:Buffers\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e:Biotech\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDG\u003c\/strong\u003e:No\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e:2 to 8℃\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSterile\u003c\/strong\u003e:No\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.RB4478-NO_LOT.pdf?v=1734159407\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.RB4478-5_x_RIPA_Buffer_IV_with_Triton-X-100_pH_7.4_5x_concentrate.pdf?v=1734159407\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44909688258741,"sku":"RB4478-500mL","price":35.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/500ml_bottle_new_v2_sm_w_21.png?v=1734512024"},{"product_id":"2xripa-buffer-iiii-with-edta-and-egta-ph-7-4-2xconcentrate-biofargo","title":"RIPA Lysis Buffer IIII with EDTA and EGTA (pH 7.4) 2 x concentrate- 500ML","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e2 x RIPA Buffer IIII with EDTA and EGTA (pH 7.4) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and\/or interference with protein immunoreactivity and biological activity. RIOA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of the this buffer. In addition, RIPA buffer minimizes non-specific protein-binding most specific interactions to occur, enabling studies of relevant protein-protein interactions.RIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X107 cells) of most adherent mammalian cell lines.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 202px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 27px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eTotal Product Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eIndividual Container Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eRefrigeration Requirements\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRefrigerator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e12161700\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eBuffers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eDG\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 22px; text-align: center;\"\u003e2 to 8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eSterile\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.RB4477-NO_LOT.pdf?v=1734159615\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.RB4477-2_x_RIPA_Buffer_IIII_with_EDTA_EGTA_pH_7.4_2x_concentrate.pdf?v=1734159615\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44909706543285,"sku":"RB4477-500mL","price":63.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/500ml_bottle_new_v2_sm_w_20.png?v=1734512022"},{"product_id":"2xripa-buffer-ii-ph-8-0-2xconcentrate-biofargo","title":"2xRIPA Lysis Buffer II (pH 8.0) 2xconcentrate-500ML","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e2 x RIPA Buffer II (pH 8.0) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and\/or interference with protein immunoreactivity and biological activity. RIOA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of the this buffer. In addition, RIPA buffer minimizes non-specific protein-binding most specific interactions to occur, enabling studies of relevant protein-protein interactions.RIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X107 cells) of most adherent mammalian cell lines.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 202px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 27px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eTotal Product Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eIndividual Container Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eRefrigeration Requirements\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRefrigerator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eGrade\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eBiotech\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eDG\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e2 to 8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eSterile\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 22px; text-align: center;\"\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 22px; text-align: center;\"\u003e12161700\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eBuffe\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.RB4476-NO_LOT.pdf?v=1734159868\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.RB4476-2_x_RIPA_Buffer_II_pH_8.0_2x_concentrate.pdf?v=1734159868\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44909721780405,"sku":"RB4476-500mL","price":35.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/500ml_bottle_new_v2_sm_w_19.png?v=1734512021"},{"product_id":"2xripa-buffer-i-ph-7-4-2xconcentrate-biofargo","title":"RIPA Lysis Buffer 2X Solution, 500ML (pH 7.4)","description":"\u003ch2\u003eIntroduction\u003c\/h2\u003e\n\u003cp\u003eRIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eExtraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and\/or interference with protein immunoreactivity and biological activity.\u003c\/p\u003e\n\u003cp\u003eRIPA Buffer enables rapid, efficient cell lysis and solubilization of protein from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of the this buffer. In addition, RIPA buffer minimizes nonspecificprotein-binding most specific interactions to occur, enabling studies of relevant \u003cbr\u003eprotein-protein interactions.\u003c\/p\u003e\n\u003cp\u003eRIPA buffer is supplied as several kinds of solution. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA buffer is sufficient to lyse cells from one 100mm culture dish (0.5 to 5X10^7 cells) of most adherent mammalian cell lines. \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eIt is recommended to store the product at 2-8℃. The product is stable for 1 year when stored properly. \u003c\/p\u003e\n\u003ch2\u003eProcedure \u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eDilute RIPA Buffers to 1X concentration before using.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eA. Procedure for Lysis of adherent cells\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRemove growth medium from the cells by decantation or aspiration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWash cells to remove residual medium. Slowly add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume being careful not to dislodge cells. Mix gently and remove the wash solution. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml for 0.5 to 5x107cells). Incubate on ice or in a refrigerator (2-8℃) for five minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRapidly scrape the plate with a cell scraper to remove and lyse residual cells. Transfer the cell lysate to a tube on ice. The lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70℃ for future use. It is best to freeze the lysate before clarification, since the freeze-thaw cycle may cause some denatured protein aggregates.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eClarify the lysate by centrifugation at 8,000 x g for 10 minutes at 4\u003c\/span\u003e\u003cspan\u003eo \u003c\/span\u003e\u003cspan\u003eC to pellet the cell debris.    Note: if a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eCarefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eB. Procedure for lysis of suspension cultured cells\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePellet the cells by centrifugation at 450 x g for 5 minutes. \u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCarefully remove the medium from the cell pellet by decantation or aspiration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWash the cells to remove residual medium. Add a volume of a physiological wash solution, such as DPBS, equal to the original medium volume. Mix or vortex briefly to resuspend the cells completely. Centrifuge for 5 minutes at 450 x g to pellet the cells and carefully remove wash solution supernatant. Repeat the wash once in order to remove any other minor contaminants. More washing steps can be done, but two is usually sufficient to remove nearly all of the contaminants. \u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter removal of the final wash solution from the cells, add an appropriate volume of RIPA Buffer (1ml per 0.5 to 5X107 cells) to the cell pellet, and mix or vortex briefly to resuspend the cells completely. Incubate on ice or in a refrigerator (2-8℃) for five minutes. Vortex briefly to resuspend and lyse residual cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe lysate can either be used immediately or quick-frozen in liquid nitrogen and stored at -70℃ for future use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eClarify the lysate by centrifugation at 8,000 X g for 10 minutes at 4℃ to pellet the cell debris.      Note: If a mucoid aggregate of denatured nucleic acids is present, carefully remove it with a micropipette before centrifugation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eCarefully transfer the supernatant containing the soluble protein to a tube on ice for immunoprecipitation or other analysis \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReference\u003c\/h2\u003e\n\u003cp\u003eAntibodies a Laboratory Manual, Harlow, E. and Lane, D., Cold Spring Harbor Press (Cold Spring Harbor, NY: 1988), p449. \u003c\/p\u003e\n\u003ch2\u003ePrecautions and Disclaimer\u003c\/h2\u003e\n\u003cp\u003eRIPA Buffer is for laboratory use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.RB4475-NO_LOT.pdf?v=1734160092\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS.RB4475-2_x_RIPA_Buffer_I_pH_7.4_2_x_concentrate.pdf?v=1734160091\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"500mL","offer_id":44909737246901,"sku":"RB4475-500mL","price":63.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/500ml_bottle_new_v2_sm_w_18.png?v=1734512019"},{"product_id":"pmsf-protease-inhibitor","title":"PMSF Protease Inhibitor","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePMSF Protease Inhibitor is used to inhibit protease activity released by broken cells during cell extraction\/cell lysate\/tissue homogenate sample collection in ELISA experiments. PMSF protease inhibitor inhibits serine proteases (e.g., chymotrypsin, trypsin, thrombin) and sulfhydryl proteases (e.g., papain).\u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eAncillary ELISA reagent\u003c\/p\u003e\n\u003cp\u003eFormat: Solution\u003c\/p\u003e\n\u003ch2\u003eHandling \u0026amp; Safety\u003c\/h2\u003e\n\u003cp\u003eStorage:: +4°C\u003c\/p\u003e\n\u003cp\u003eShipping: +4°C (International: +4°C)\u003c\/p\u003e","brand":"DULAI","offers":[{"title":"10 mL","offer_id":52209491771573,"sku":"E-EL-SR002","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/biofargo.jpg?v=1761012618"}],"url":"https:\/\/biofargo.com\/collections\/protein-extraction-lysis.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}