{"title":"Probe RT-qPCR Kits","description":"","products":[{"product_id":"bvdv-detection-kit-shentek®","title":"BVDV RT-qPCR Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® BVDV Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay format \/ reaction volume\u003c\/td\u003e\n\u003ctd\u003e20 µL total reaction volume (10 µL RT-qPCR MIX + 10 µL extracted sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT-qPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eBVDV Primer \u0026amp; Probe MIX 3.7 µL; 5×RT-qPCR Buffer 4 µL; RT-qPCR Enzyme MIX 1 µL; IPC MIX 1.3 µL; Total 10 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 45 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during anneal\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eNo cross-amplification with bovine, porcine genomes, cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or expression bacteria (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls and acceptance criteria (Ct thresholds)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; NCS: FAM Ct ≥ 40 or no amplification; PCS: FAM Ct \u0026lt; 35 and amplification. Test sample interpretation uses FAM and VIC channels: Positive if FAM Ct \u0026lt; 40 (specific amplification); Presumptive positive if FAM ≥ 40 with VIC \u0026lt; 40 (indicates inhibitors); Negative if FAM ≥ 40 and VIC \u0026lt; 40 absent.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage and stability\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore components at –20 °C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and most standard real-time PCR systems supporting probe assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification observed with bovine\/porcine genomes, common cell lines or typical expression organisms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesigned for complex matrices:\u003c\/strong\u003e Validated for detection in cell cultures and bovine serum when used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Includes IPC MIX to monitor for PCR inhibition and sample\/extraction performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix and enzyme\/buffer mixes reduce setup variability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with multiple common real-time PCR instruments and standard probe detection settings (FAM for target, VIC for IPC).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BVDV target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BVDV nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing BVDV target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBVDV Primer \u0026amp; Probe MIX (NNC091)\u003c\/span\u003e\u003cstrong\u003e200 µL × 1 tube; –20 °C; protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX (NNC079)\u003c\/span\u003e\u003cstrong\u003e100 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (NNC066)\u003c\/span\u003e\u003cstrong\u003e150 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer (NNB018)\u003c\/span\u003e\u003cstrong\u003e300 µL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e1.2 mL × 1 tube; store nuclease-free (room temperature or –20 °C as per lab practice)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic human use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction in a biosafety cabinet; follow BSL-2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and certified RNase\/DNase-free reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect light-sensitive reagents (primer \u0026amp; probe mix) from light.\u003c\/p\u003e\n\u003cp\u003e• If VIC (IPC) signal indicates inhibition, repeat sample preparation or remove inhibitors and retest.\u003c\/p\u003e\n\u003cp\u003e• Check expiration dates on labels; store components at –20 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506731.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506731.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is 50 copies per reaction (LOD = 50 copies\/reaction).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: What samples can be tested with this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for testing cell culture samples (≤10^7 cells), bovine serum and other sample types after appropriate extraction with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit. For special sample matrices consult technical support.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended reaction and thermal cycling conditions?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eUse a 20 µL total reaction (10 µL RT-qPCR MIX + 10 µL extracted sample). Thermal program: Reverse transcription 50 °C 15:00; Activation 95 °C 00:30; 45 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence during annealing\/extension).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the internal control (VIC) is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf VIC signal is inhibited, repeat sample preparation or apply inhibitor removal steps during extraction and retest the sample.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45187313631413,"sku":"1506731","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BVDV_Detection_Kit.jpg?v=1742276076"},{"product_id":"pi-3-detection-kit-shentek™","title":"PI-3 RT-qPCR Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThe SHENTEK® PI-3 Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay type\u003c\/td\u003e\n\u003ctd\u003eQualitative RT-qPCR (probe-based)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended samples\u003c\/td\u003e\n\u003ctd\u003eCell banks, virus seed banks, bovine serum (other matrices consult technical support)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volume\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e20 μL total per reaction (10 μL RT-qPCR MIX + 10 μL extracted sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT-qPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003ePI-3 Primer \u0026amp; Probe MIX 3.7 μL; 5×RT-qPCR Buffer 4 μL; RT-qPCR Enzyme MIX 1 μL; IPC MIX 1.3 μL; total 10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 00:30 (1 cycle); Denaturation: 95 °C 00:15 (45 cycles); Annealing\/extension: 60 °C 01:00 (45 cycles, fluorescence read during this step)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCt interpretation criteria (controls)\u003c\/td\u003e\n\u003ctd\u003eNTC: FAM Ct ≥ 40.00 or no amplification; PCS: FAM Ct \u0026lt; 35.00 and VIC Ct \u0026lt; 35.00 for duplicate runs; NCS: FAM Ct ≥ 40.00 and VIC Ct \u0026lt; 35.00 for duplicate runs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCt interpretation criteria (test samples)\u003c\/td\u003e\n\u003ctd\u003ePositive: FAM Ct \u0026lt; 40.00 (at least one well) with specific amplification and VIC Ct \u0026lt; 40.00 (duplicate runs); Presumptive positive with PCR inhibitors: FAM Ct ≥ 40.00 and VIC Ct \u0026lt; 40.00; Negative: FAM Ct ≥ 40.00 (no amplification) and VIC Ct \u0026lt; 40.00; Not conclusive if VIC inhibited (repeat extraction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility (instruments)\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, CFX96 and other real-time PCR systems supporting probe chemistry\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check label expiration date)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack size \/ Reactions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity down to 50 copies per reaction (LOD).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification observed with bovine\/porcine genomes, common engineered cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or common production microbes (E. coli, Pichia pastoris).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated controls:\u003c\/strong\u003e Includes internal positive control (IPC) and recommended positive\/negative controls to monitor extraction and amplification performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized master mix:\u003c\/strong\u003e Ready-to-use primer\/probe mix and RT-qPCR enzyme\/buffer mixes for streamlined setup and consistent performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with standard workflows:\u003c\/strong\u003e Designed to be used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit and compatible with common real-time PCR instruments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuitable for complex matrices:\u003c\/strong\u003e Validated for detection in cell culture and bovine serum; extraction workflow recovers PI-3 RNA from complex samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of PI-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for PI-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing PI-3 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePI-3 Primer \u0026amp; Probe MIX (NNC092)\u003c\/span\u003e\u003cstrong\u003e200 μL × 1 tube; –20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX (NNC079)\u003c\/span\u003e\u003cstrong\u003e100 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (NNC066)\u003c\/span\u003e\u003cstrong\u003e150 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer (NNB018)\u003c\/span\u003e\u003cstrong\u003e300 μL × 1 tube; –20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008)\u003c\/span\u003e\u003cstrong\u003e1.2 mL × 1 tube; store at –20 °C or as recommended\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedures in a biosafety cabinet following BSL-2 guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free (RNase\/DNase-free) consumables and reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer\/probe mix from light.\u003c\/p\u003e\n\u003cp\u003e• Follow SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit user guide (Product No. 1506730) for sample preparation and extraction.\u003c\/p\u003e\n\u003cp\u003e• If VIC (IPC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat assay.\u003c\/p\u003e\n\u003cp\u003e• Do not use beyond expiration date printed on the kit labels.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506732.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506732.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity (LOD) of the SHENTEK PI-3 Detection Kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity (limit of detection) is 50 copies per reaction when used with the recommended extraction procedure.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported by the kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for detection of PI-3 in cell culture samples (≤10^7 cells) and bovine serum. For other sample matrices, consult technical support.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be run with each assay?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude a No Template Control (NTC), a Negative Control Sample (NCS), and a Positive Control Sample (PCS). Use duplicates for each control and test sample as recommended.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the internal positive control (VIC) is inhibited?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf the VIC IPC signal is inhibited, repeat sample extraction with measures to remove inhibitors (e.g., additional purification) and retest the sample.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45188914905269,"sku":"1506732","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PI-3_Detection_Kit.jpg?v=1742280533"},{"product_id":"pi-3-positive-control-shentek®","title":"PI-3 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: 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#c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® PI-3 Positive Control is an armored RNA positive control intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and SHENTEK® PI-3 \/ Bovine Virus Detection Kits. It is designed to monitor the entire process of PI-3 RNA extraction and subsequent RT‑PCR detection, serving as a process\/control sample to validate extraction efficiency and RT‑PCR performance. The product is supplied as ready-to-use aliquots and is formulated to be non-infectious (armored RNA) while mimicking target viral RNA during extraction and amplification workflows.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eArmored RNA positive control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume per tube\u003c\/td\u003e\n\u003ctd\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of tubes\u003c\/td\u003e\n\u003ctd\u003e3 tubes per pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore below -65 °C; stable for up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess\/positive control for PI-3 RNA extraction and RT‑PCR detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatibility\u003c\/td\u003e\n\u003ctd\u003eCompatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit, SHENTEK® PI-3 Detection Kit, and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUse preparation\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReady for direct use without dilution; thaw and equilibrate before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSafety\u003c\/td\u003e\n\u003ctd\u003eArmored RNA (non-infectious surrogate); handle in biosafety cabinet and autoclave experimental waste\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull-process positive control:\u003c\/strong\u003e Monitors both RNA extraction and RT‑PCR detection steps to validate workflow integrity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA formulation:\u003c\/strong\u003e Non-infectious armored RNA that mimics native viral RNA behavior during extraction and amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Supplied at working concentration — no dilution required prior to use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term stability:\u003c\/strong\u003e Stable when stored below -65 °C for up to 12 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKit compatibility:\u003c\/strong\u003e Designed for seamless use with SHENTEK® extraction and detection kits for PI-3 and bovine virus panels.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-use aliquots:\u003c\/strong\u003e Provided as 100 μL tubes to minimize freeze–thaw and cross-contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eProcess control for PI-3 RNA extraction workflows\u003c\/li\u003e\n\u003cli\u003ePositive control for RT‑PCR assays targeting PI-3\u003c\/li\u003e\n\u003cli\u003eQuality control and validation of SHENTEK® extraction and detection kits\u003c\/li\u003e\n\u003cli\u003eOperator training and proficiency testing\u003c\/li\u003e\n\u003cli\u003eInter-run and lot-to-lot performance monitoring\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePI-3 Positive Control (armored RNA), 100 μL × 3 tubes\u003c\/span\u003e\u003cstrong\u003eStore below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (3 control tubes, 100 μL each) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex and spin briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation protocol described in the user guide of the SHENTEK® PI-3 Detection Kit and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3).\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution.\u003c\/p\u003e\n\u003cp\u003e• Use only after equilibrating to room temperature; avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different production batches.\u003c\/p\u003e\n\u003cp\u003e• Do not use reagents beyond the expiration date shown on the label.\u003c\/p\u003e\n\u003cp\u003e• Although armored RNA is non-infectious, operate in a biosafety cabinet where possible; sterilize experimental waste by autoclaving and dispose of according to medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506738.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506738.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of the SHENTEK® PI-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIt is intended as a process\/positive control to monitor PI-3 RNA extraction and RT‑PCR detection workflows when used with SHENTEK® extraction and detection kits.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the control be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the control below -65 °C. It is stable for up to 12 months; always check the expiration date on the label.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Do I need to dilute the PI-3 Positive Control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. The product is supplied ready for direct use without dilution. Thaw, vortex, briefly spin, and equilibrate to room temperature before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the PI-3 Positive Control infectious and are special biosafety procedures required?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe control is an armored RNA surrogate and is non-infectious; however, it is recommended to handle it in a biosafety cabinet when possible and to autoclave\/dispose of experimental waste according to institutional regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45188968054965,"sku":"1506738","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PI-3_Positive_Control.jpg?v=1742282642"},{"product_id":"reo-3-detection-kit-shentek®","title":"REO-3 Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® REO-3 Analysis Kit is a qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Reovirus 3 (REO-3) contamination in cell banks, virus seed banks and bovine serum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay sensitivity \/ Limit of detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification with bovine, porcine genomes, common production\/engineering cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volume\u003c\/td\u003e\n\u003ctd\u003e20 μL total reaction volume (10 μL RT-qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 00:30 (1 cycle); Denaturation: 95 °C 00:15; Annealing\/extension: 60 °C 01:00; 45 cycles (fluorescence read during annealing\/extension)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls and interpretation thresholds\u003c\/td\u003e\n\u003ctd\u003eControls: NTC, NCS, PCS, Internal Positive Control (IPC). Typical Ct acceptance criteria: FAM (target): Positive if Ct \u0026lt; 40.00 (at least one well) or Ct \u0026lt; 40 with duplicate amplification; VIC (IPC): Ct \u0026lt; 35.00 indicates valid IPC amplification. NTC\/NCS: no specific amplification (Ct ≥ 40 or absent) required. See user guide tables for full interpretation rules.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eStore components at -20 °C (recommended). Kit components stable for up to 24 months; items with recommended storage at -20 °C should be maintained within ±10 °C (–30 °C to –10 °C).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and other real-time PCR systems supporting probe assays and user-defined cycling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity down to 50 copies per reaction (LOD).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification with common cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or bacterial\/yeast genomes (E. coli, Pichia pastoris), and with bovine\/porcine genomes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComplete RT-qPCR solution:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, RT-qPCR enzyme mix, buffer and internal positive control mix for end-to-end detection when combined with recommended extraction kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with multiple instruments:\u003c\/strong\u003e Validated on several common real-time PCR platforms and supports standard probe-based RT-qPCR setup.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesigned for complex matrices:\u003c\/strong\u003e Optimized to detect REO-3 RNA from cells and bovine serum when used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal control included:\u003c\/strong\u003e IPC MIX provided to monitor extraction and PCR inhibition.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of REO-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for REO-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing REO-3 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eREO-3 Primer \u0026amp; Probe MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (200 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT-qPCR Enzyme MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C (100 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eIPC MIX (Internal Positive Control MIX)\u003c\/span\u003e\u003cstrong\u003e-20 °C (150 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT-qPCR Buffer\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O\u003c\/span\u003e\u003cstrong\u003eRoom temperature or 2-8 °C as supplied (1.2 mL × 1 tube) — follow label instructions\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eKit format \/ quantity\u003c\/span\u003e\u003cstrong\u003eReagents for 50 reactions\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction in a biosafety cabinet and follow BSL-2 safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and certified RNase\/DNase-free reagents and tips to avoid contamination.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit completely at 2–8 °C or on ice, vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect primer \u0026amp; probe mixes from light.\u003c\/p\u003e\n\u003cp\u003e• Store components at -20 °C; short-term storage below -30 °C is acceptable if supported by stability data.\u003c\/p\u003e\n\u003cp\u003e• Check expiration dates on labels prior to use.\u003c\/p\u003e\n\u003cp\u003e• If IPC (VIC) signal indicates inhibition, repeat sample preparation or remove inhibitors and repeat assay.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506733.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506733.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's limit of detection (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eValidated sample types include cell culture (up to 1×10^7 cells per 100–200 μL) and bovine serum. For other matrices consult technical support and use the recommended SHENTEK® extraction kit.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls are required and how are results interpreted?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eRun NTC (No Template Control), NCS (Negative Control Sample), PCS (Positive Control Sample) and IPC. Typical acceptance thresholds: FAM target Ct \u0026lt; 40 indicates positive amplification; IPC (VIC) Ct \u0026lt; 35 indicates successful internal control amplification. Follow the user guide control tables for detailed interpretation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eCompatible with common real-time PCR systems including SHENTEK-96S, LightCycler 480 II, ABI 7500, Bio-Rad CFX96 and other instruments that support probe-based RT-qPCR.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45188988895413,"sku":"1506733","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/REO-3_Detection_Kit.jpg?v=1742283552"},{"product_id":"reo-3-positive-control-shentek®","title":"REO-3 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eDetection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® REO-3 Positive Control is an armored RNA positive control intended for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction and Detection Kits. It is supplied to monitor the entire process of REO-3 RNA extraction and RT-PCR detection assays. The product is provided ready for direct use (no dilution required) and supplied as 100 μL × 3 tubes. The control supports assay process monitoring, method validation and routine quality control. The product is for research use only and not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd\u003eArmored RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume per tube\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage condition\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eBelow -65 °C; stable for up to 12 months (check expiration label)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003ePositive process control for REO-3 RNA extraction and RT-PCR detection assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUsage\u003c\/td\u003e\n\u003ctd\u003eReady for direct use without dilution; follow Positive Control Sample (PCS) preparation protocol in the corresponding SHENTEK detection kit user guide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eFull-process monitoring:\u003c\/strong\u003e Designed to monitor the entire workflow from RNA extraction through RT-PCR detection for REO-3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA format:\u003c\/strong\u003e Provides increased stability and safety compared with naked RNA; mimics viral RNA during extraction and detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady-to-use:\u003c\/strong\u003e Supplied ready for direct use without the need for dilution when prepared according to kit instructions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit, SHENTEK® REO-3 Detection Kit, and SHENTEK® Bovine Virus Detection Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eProcess control for REO-3 RNA extraction workflows\u003c\/li\u003e\n\u003cli\u003ePositive control for RT-PCR assay validation and routine QC\u003c\/li\u003e\n\u003cli\u003eAssay performance troubleshooting and operator training\u003c\/li\u003e\n\u003cli\u003eCompatibility testing with SHENTEK extraction and detection reagents\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eREO-3 Positive Control\u003c\/span\u003e\u003cstrong\u003e100 μL × 3 tubes; store below -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: N\/A (3 tubes of 100 μL each; used as positive control material) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Intended for research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex thoroughly and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Follow the Positive Control Sample (PCS) preparation protocol in the SHENTEK® REO-3 Detection Kit or SHENTEK® Bovine Virus Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• This product is ready for direct use without dilution when prepared per instructions.\u003c\/p\u003e\n\u003cp\u003e• Equilibrate to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n\u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Although armored RNA is safer than naked RNA, it is recommended to handle the product in a biosafety cabinet.\u003c\/p\u003e\n\u003cp\u003e• Experimental waste should be sterilized by autoclave and disposed of in accordance with medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506737.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506737.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the SHENTEK® REO-3 Positive Control?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore the product below -65 °C. Under appropriate storage it is stable for up to 12 months; always check the expiration date on the label.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Do I need to dilute the positive control before use?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is provided ready for direct use. Follow the PCS preparation protocol in the appropriate SHENTEK detection kit user guide.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the recommended thawing procedure?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThaw one tube completely at 2–8 °C or on ice, vortex thoroughly and briefly centrifuge prior to use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this product be used for clinical diagnostics?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for research use only (RUO) and is not for clinical diagnostic or therapeutic use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg fill=\"currentColor\" viewbox=\"0 0 20 20\" class=\"shentek-notice-icon\"\u003e\n \u003cpath clip-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" fill-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45188999217333,"sku":"1506737","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/REO-3_Positive_Control.jpg?v=1742283721"},{"product_id":"bovine-virus-detection-multiplex-kit-bvdv-reo-3-pi-3-bpv-3-bav-3","title":"Bovine Virus Analysis Multiplex Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Bovine Virus Analysis Multiplex Kit (BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3) is a multiplex real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the simultaneous molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and multiplexed analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended reactions\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 Reactions (kit provides sufficient reagents for 50 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction volumes\u003c\/td\u003e\n\u003ctd\u003eRT-qPCR: 20 μL total (10 μL RT-qPCR MIX + 10 μL sample). qPCR (BPV-3\/BAV-3): 30 μL total (20 μL qPCR MIX + 10 μL sample).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling (RT-qPCR for BVDV\/REO-3\/PI-3)\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 40 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during annealing\/extension).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling (qPCR for BPV-3\/BAV-3)\u003c\/td\u003e\n\u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of Denaturation 95 °C 00:15 and Annealing\/Extension 60 °C 01:00 (fluorescence read during annealing\/extension).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReporter dyes \/ Targets\u003c\/td\u003e\n\u003ctd\u003eREO-3: FAM; PI-3: HEX; BVDV: CY5; BPV-3: FAM; BAV-3: CY5; Internal Control (IC): ROX.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — controls\u003c\/td\u003e\n\u003ctd\u003eNTC: Undetected (no specific amplification); NCS: Undetected; PCS: Ct \u0026lt; 35.00 with specific amplification; ROX (IC) for controls: Ct \u0026lt; 35.00.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance criteria — test samples\u003c\/td\u003e\n\u003ctd\u003ePositive call: specific amplification with Ct \u0026lt; 40.00 in the relevant fluorescence channel. Sample inhibition check: compare sample ROX Ct to NCS ROX Ct; difference \u0026gt; 2 indicates possible inhibition. If Ctsample - CtNCS \u0026lt; 2 then negative; ≥ 2 not conclusive (PCR inhibitors possible).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S Real-time PCR System, LightCycler 480Ⅱ, ABI 7500 (other real-time PCR instruments may be used with appropriate setup).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls included \/ recommended\u003c\/td\u003e\n\u003ctd\u003eInternal Control (VIR IC) provided in kit; Positive Controls for individual targets sold separately (Product Nos. 1506736, 1506737, 1506738, 1506746, 1506739).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMultiplex detection:\u003c\/strong\u003e Simultaneous detection of three RNA viruses (BVDV\/REO-3\/PI-3) or two DNA viruses (BPV-3\/BAV-3) in a single well using multiplex real-time PCR.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal control provided:\u003c\/strong\u003e VIR Internal Control (IC) included to monitor extraction and amplification performance and identify inhibition.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible with standard instruments:\u003c\/strong\u003e Validated on SHENTEK-96S, LightCycler 480Ⅱ and ABI 7500; adaptable to other real-time PCR platforms.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized workflow for complex matrices:\u003c\/strong\u003e Designed to work with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for efficient recovery from serum and cell matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for BVDV\/REO-3\/PI-3\/BPV-3\/BAV-3 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing nucleic acids target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×Multiplex RT-PCR Buffer (NNB020), 200 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex RT-PCR Enzyme MIX (NNC102), 100 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex qPCR Reaction Buffer (NNB021), 850 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (NNA057), 600 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase-Free H2O (NND008), 1.2 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months) or as label indicates\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBV RNA Primer \u0026amp; Probe MIX (NNC103), 170 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months); protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBV DNA Primer \u0026amp; Probe MIX (NNC104), 120 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months); protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMultiplex IPC MIX (NNC105), 170 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (stable for 24 months)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: Multiplex RT-qPCR \/ qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n\u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\n\u003cp\u003e• Handle positive controls and extraction procedures in a biological safety cabinet following BSL-2 guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and reagents certified RNase\/DNase-free.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components completely at 2–8 °C or on ice, vortex thoroughly and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• Protect probe-containing reagents from light.\u003c\/p\u003e\n\u003cp\u003e• Check control acceptance criteria (NTC\/NCS\/PCS\/IC Ct thresholds) for run validity.\u003c\/p\u003e\n\u003cp\u003e• If ROX (IC) Ct(sample) - ROX Ct(NCS) \u0026gt; 2, investigate potential PCR inhibition and repeat extraction\/dilution as needed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506741.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506741.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for bovine serum and cell culture matrices (cell banks). Use SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for nucleic acid extraction prior to detection.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has a reported limit of detection of 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: What controls should be included in each run?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), and Positive Control Sample (PCS). An internal control (VIR IC) is provided to monitor extraction and amplification; add IC during extraction or to the reaction mix if not already added.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are positive\/negative results determined?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eA positive result is a well showing specific amplification with Ct \u0026lt; 40. For controls, PCS and ROX (IC) should have Ct \u0026lt; 35. Compare ROX Ct of sample to NCS to assess inhibition (difference \u0026gt; 2 suggests inhibition).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189025562805,"sku":"1506741","price":3399.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Bovine_Virus_Detection_Multiplex_Kit_BVDV_REO-3_PI-3_BPV-3_BAV-3.jpg?v=1742285396"},{"product_id":"vesivirus-2117-detection-kit-shentek®","title":"Vesivirus 2117 Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Vesivirus 2117 Analysis Kit is a qualitative RT‑qPCR assay for Analysis of Vesivirus 2117 contamination in mammalian cell lines (e.g., CHO) and related sample matrices used in biopharmaceutical production. The kit is designed to be used together with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730). The assay includes a Vesivirus 2117 Positive Control and a VIR Internal Control (IC) to monitor extraction and amplification. The method achieves a sensitivity of 50 copies per reaction, demonstrates high specificity without cross‑amplification of a range of cell line and microbial genomes, and is suitable for detecting viral RNA from complex matrices such as cells and serum. For Research Use Only.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Vesivirus 2117 RNA in mammalian cell lines and related samples (e.g., cell culture, bovine serum).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eSensitivity of 50 copies per reaction (reported assay sensitivity).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eHigh specificity; no cross‑amplification observed with genomes from CHO, VERO, 293T, MDCK, NS0, Sf9, E. coli, Pichia pastoris and other tested bovine\/porcine\/engineering cell lines.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample types\u003c\/td\u003e\n\u003ctd\u003eCell culture (≤10^7 cells), bovine serum, and other matrices amenable to SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction format \/ volume\u003c\/td\u003e\n\u003ctd\u003e20 µL total reaction volume (10 µL RT‑qPCR MIX + 10 µL sample\/controls); per reaction RT‑qPCR MIX composition provided (10 µL final MIX).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT‑qPCR mix composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003e5×RT‑qPCR Buffer 4 µL, RT‑qPCR Enzyme MIX 1 µL, VV‑2117 Primer\u0026amp;Probe MIX 3.3 µL, VIR IPC MIX 1.7 µL, (optionally) VIR Internal Control (IC) 0.5 µL; final volume 10 µL RT‑qPCR MIX.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eReverse transcription 50 °C for 15:00 (1 cycle); Activation 95 °C for 0:30 (1 cycle); 40 cycles of 95 °C for 0:15 and 60 °C for 1:00 (fluorescence read during 60 °C).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance \/ interpretation thresholds\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eControl acceptance: FAM and VIC Ct \u0026lt; 35.0 for PCS; NTC and NCS: undetected in FAM and Ct \u0026lt; 35.0 in VIC. Test sample calls: FAM Ct \u0026lt; 40.0 (at least one replicate) with specific amplification = Positive. Negative defined as undetected FAM and VIC sample Ct difference to NCS (Ctsample − CtNCS) \u0026lt; 2. If Ctsample − CtNCS ≥ 2, result is not conclusive (possible inhibition).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls included \/ recommended\u003c\/td\u003e\n\u003ctd\u003eVesivirus 2117 Positive Control (PCS) — separate product (1506743‑R01) recommended; VIR Internal Control (IC) and VIR IPC MIX included to monitor extraction\/amplification. No Template Control (NTC) and Negative Control Sample (NCS) required.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eReagents for 50 reactions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months; check expiration on labels.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended instruments\u003c\/td\u003e\n\u003ctd\u003eCompatible with SHENTEK‑96S, LightCycler 480Ⅱ, ABI 7500 and other real‑time PCR systems supporting probe assays (FAM\/VIC detection).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExtraction\u003c\/td\u003e\n\u003ctd\u003eDesigned to be used with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit (Product No. 1506730); automated extraction on rHCDpurify system recommended but manual extraction possible per extraction kit guide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity reported at 50 copies per reaction for reliable detection of low‑level contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross‑amplification with a panel of mammalian cell lines and common engineering microorganisms tested.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInternal\/extraction controls:\u003c\/strong\u003e Includes VIR Internal Control (IC) and VIR IPC MIX to monitor extraction efficiency and PCR inhibition; Positive Control provided as separate product.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on SHENTEK‑96S, LightCycler 480Ⅱ and ABI 7500; general probe‑based qPCR instruments supported.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized for complex matrices:\u003c\/strong\u003e Combined workflow with SHENTEK extraction kit enables efficient recovery of viral RNA from cells and serum.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eMolecular evaluation of Vesivirus 2117 target sequences in mammalian cell substrates (e.g., CHO) utilized in biopharmaceutical manufacturing\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control and molecular monitoring of cell culture systems and in vitro cell culture supplements\u003c\/li\u003e\n\u003cli\u003eIn-process analytical evaluation and biomanufacturing root cause analysis workflows\u003c\/li\u003e\n\u003cli\u003eIn vitro research studies requiring the qualitative molecular analysis of Vesivirus 2117 target RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (NNA057) 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003e5×RT‑qPCR Buffer (NNB018) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRNase‑Free H2O (NND008) 1.2 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (or as labelled)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eRT‑qPCR Enzyme MIX (NNC079) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVV‑2117 Primer \u0026amp; Probe MIX (NNC123) 170 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (NNC107) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore at -20 °C (check label)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: RT-qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For Research Use Only (not for diagnostic or therapeutic use).\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate protective eyewear, gloves, mask and lab clothing.\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction steps in a biosafety cabinet and follow BSL‑2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease‑free consumables; avoid RNase\/DNase contamination.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit reagents completely at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\n\u003cp\u003e• Protect enzyme mixes from light; store components at recommended temperatures and check expiration dates on labels.\u003c\/p\u003e\n\u003cp\u003e• Include NTC, NCS and PCS controls and run duplicates as recommended; follow acceptance criteria for Ct interpretation.\u003c\/p\u003e\n\u003cp\u003e• If inhibition is suspected (VIC Ct difference ≥ 2 vs NCS), retest after removing inhibitors or repeat extraction.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506743.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506743.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe assay sensitivity is reported at 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for mammalian cell culture samples (e.g., CHO cells, ≤10^7 cells) and bovine serum when used with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit; other complex matrices may be used following appropriate extraction and validation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret Ct values?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eA sample is positive when FAM Ct \u0026lt; 40.0 (at least one replicate) with specific amplification. PCS, NCS and NTC acceptance criteria and VIC internal control thresholds are provided in the user guide; if the VIC Ct difference indicates inhibition (Ctsample − CtNCS ≥ 2), the result is not conclusive and re‑extraction or inhibitor removal is recommended.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at −20 °C as indicated on labels. Components are stable for up to 24 months under appropriate storage conditions; always check the expiration date on tube labels.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189071569077,"sku":"1506743","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Vesivirus_2117_Detection_Kit.jpg?v=1742287223"},{"product_id":"vesivirus-2117-positive-control-shentek®","title":"Vesivirus 2117 Positive Control SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-65 °C (stable for 12 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® Vesivirus 2117 Analysis Kit is an RT-qPCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of Vesivirus 2117 target sequences in mammalian cell lines (e.g., CHO) and related sample matrices utilized in biopharmaceutical production. The kit is optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730). The assay includes a Vesivirus 2117 Positive Control and an Internal Process Control (IPC) to monitor nucleic acid recovery and amplification. The method achieves a limit of testing (LoD) of 50 copies per reaction, demonstrates high analytical specificity without cross-amplification against a range of cell line and non-target genomes, and is highly suitable for the analysis of target RNA from complex matrices such as cell suspensions and in vitro cell culture supplements. Strictly for Industrial Bioprocessing Quality Control and In Vitro Analytical Use Only.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eForm\u003c\/td\u003e\n\u003ctd\u003eArmored RNA positive control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e100 μL × 3 tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage temperature and stability\u003c\/td\u003e\n\u003ctd\u003eStored below -65 °C; stable up to 12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eProcess control for Vesivirus 2117 RNA extraction and RT‑PCR detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003eNot specified in the document\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDilution requirement\u003c\/td\u003e\n\u003ctd\u003eReady for direct use without dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHandling\u003c\/td\u003e\n\u003ctd\u003eHandle in a biosafety cabinet; avoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eProcess monitoring:\u003c\/strong\u003e Monitors the entire Vesivirus 2117 RNA extraction and RT‑PCR workflow (extraction → detection).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReady to use:\u003c\/strong\u003e Provided as a ready-to-use positive control; no dilution required before use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eArmored RNA:\u003c\/strong\u003e Stabilized armored RNA format improves nuclease resistance and mimics viral particles during extraction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term stability:\u003c\/strong\u003e Stable when stored below -65 °C for up to 12 months.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety guidance:\u003c\/strong\u003e Intended to be used in a biosafety cabinet; experimental waste must be autoclaved prior to disposal.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eMolecular evaluation of Vesivirus 2117 target sequences in mammalian cell substrates (e.g., CHO) utilized in biopharmaceutical manufacturing\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control and molecular monitoring of cell culture systems and in vitro cell culture supplements\u003c\/li\u003e\n\u003cli\u003eIn-process analytical evaluation and biomanufacturing root cause analysis workflows\u003c\/li\u003e\n\u003cli\u003eIn vitro research studies requiring the qualitative molecular analysis of Vesivirus 2117 target RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVesivirus 2117 Positive Control\u003c\/span\u003e\u003cstrong\u003eBelow -65 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 3 tubes (100 μL each) | Method: RT-PCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• This product is intended for research use only and is not for clinical diagnosis or treatment.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Thaw one tube completely at 2–8 °C or on ice, then vortex and spin briefly prior to use.\u003c\/p\u003e\n\u003cp\u003e• Follow the positive control sample (PCS) preparation protocol in the SHENTEK® Vesivirus 2117 Detection Kit user guide.\u003c\/p\u003e\n\u003cp\u003e• Product is ready for direct use without dilution; equilibrate to room temperature before use.\u003c\/p\u003e\n\u003cp\u003e• Avoid repeated freeze-thaw cycles and do not mix reagents from different batches.\u003c\/p\u003e\n\u003cp\u003e• Do not use reagents beyond their expiration date; check label for expiry.\u003c\/p\u003e\n\u003cp\u003e• Handle armored RNA material in a biosafety cabinet; sterilize experimental waste by autoclaving and dispose of per medical waste regulations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506743-R01.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506743-R01.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is the Vesivirus 2117 Positive Control ready to use or does it require dilution?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe product is ready for direct use without dilution. Thaw as instructed, vortex and spin briefly before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the positive control be stored and what is its shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore below -65 °C. The product can be stored under appropriate conditions for up to 12 months; check the label for the expiration date.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this positive control be used for clinical diagnostic purposes?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for Research Use Only (RUO) and is not for clinical diagnosis or treatment.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What biosafety and waste disposal precautions are required when using this product?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eHandle the armored RNA positive control in a biosafety cabinet. Experimental waste must be sterilized via autoclaving and disposed of in compliance with medical waste regulations.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100μL × 3 tubes","offer_id":45189073436853,"sku":"1506743-R01","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Vesivirus_2117_Positive_Control.jpg?v=1742287522"},{"product_id":"porcine-circovirus-pcv-2-detection-kit-shentek®","title":"Porcine Circovirus (PCV-2) Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAnalysis Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003eStorage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003eProduct Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eThe SHENTEK® PCV-1 Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of bioprocessing inputs, such as in vitro cell culture supplements and cell banks. Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), this assay ensures the efficient recovery and analysis of target nucleic acids from complex industrial matrices.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use\u003c\/td\u003e\n\u003ctd\u003eQualitative detection of Porcine Circovirus Type 2 (PCV-2) contamination in cell banks, cell culture, trypsin and serum (research use only).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit size\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 reactions (Product No. 1506745).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e50 copies per reaction.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection chemistry \/ format\u003c\/td\u003e\n\u003ctd\u003eProbe-based real-time quantitative PCR (qPCR), dual-channel reporting (FAM for PCV-2 target, VIC for internal control).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\n\u003ctd\u003ePrepare qPCR MIX then combine 20 µL qPCR MIX + 10 µL sample (extracted nucleic acid or control) for a 30 µL final reaction volume.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n\u003ctd\u003eqPCR Reaction Buffer 15.9 µL; PCV-2 Primer \u0026amp; Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (if VIR Internal Control not added during extraction, add 0.5 µL VIR Internal Control to MIX). Total qPCR MIX volume: 20 µL (test samples).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n\u003ctd\u003eActivation: 95 °C 10:00 (mm:ss) ×1; Denaturation: 95 °C 00:15; Annealing\/Extension: 60 °C 01:00; 40 cycles. Fluorescence read during annealing\/extension.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcceptance \/ result interpretation thresholds\u003c\/td\u003e\n\u003ctd\u003eControls: NTC — FAM undetected; VIC Ct \u0026lt; 35 or undetected depending on control. Test sample positive: FAM Ct \u0026lt; 40 (specific amplification) and VIC Ct \u0026lt; 35 for duplicates. Negative: FAM undetected and Ctsample - CtNCS \u0026lt; 2. If Ctsample - CtNCS ≥ 2 or VIC inhibited, result not conclusive and repeat extraction or remove inhibitors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecificity\u003c\/td\u003e\n\u003ctd\u003eNo cross-amplification observed with genomes from bovine, porcine, cell lines (CHO, Vero, 293T, MDCK, NS0, Sf9) or engineering bacteria (E. coli, Pichia pastoris).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible instruments\u003c\/td\u003e\n\u003ctd\u003eSHENTEK-96S Real-time PCR System; LightCycler 480 II; ABI 7500 Real-Time PCR System; other qPCR instruments supporting probe-based assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable up to 24 months; follow label expiry and protect primer\/probe mix from light).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControls provided \/ required\u003c\/td\u003e\n\u003ctd\u003eNo Template Control (NTC), Negative Control Sample (NCS), Positive Control Sample (PCS) recommended; VIR Internal Control provided to monitor extraction\/PCR inhibition.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended sample types\u003c\/td\u003e\n\u003ctd\u003eCell culture (≤10^7 cells per 100–200 µL), bovine serum, trypsin and other biological matrices (use SHENTEK Virus DNA \u0026amp; RNA Extraction Kit for sample prep).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection down to 50 copies per reaction enabling sensitive detection of PCV-2 contamination.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-amplification reported with multiple mammalian cell lines and common engineering microbes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated internal control:\u003c\/strong\u003e VIR Internal Control monitors extraction efficiency and PCR inhibition (VIC channel).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, LightCycler 480 II, ABI 7500) and adaptable to other probe-based qPCR instruments.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConvenient workflow:\u003c\/strong\u003e Ready-to-use primer\/probe mix and supplied reaction buffer simplify setup; designed to be used with SHENTEK Virus DNA \u0026amp; RNA Extraction Kit for robust sample processing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eQualitative molecular analysis of PCV-2 target sequences in industrial cell banks\u003c\/li\u003e\n\u003cli\u003eAnalytical quality control of biological seed lots\u003c\/li\u003e\n\u003cli\u003eMolecular evaluation of in vitro cell culture supplements for PCV-2 nucleic acids\u003c\/li\u003e\n\u003cli\u003eIn-process monitoring and lot-release analysis for biological manufacturing\u003c\/li\u003e\n\u003cli\u003eIn vitro research projects assessing PCV-2 target sequences in complex culture matrices\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003ePCV-2 Primer \u0026amp; Probe MIX (Part No. NNC122) 120 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR IPC MIX (Part No. NNC107) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eVIR Internal Control (IC) (Part No. NNA057) 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (recommended -20 °C)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• For research use only (RUO). Not for human or diagnostic clinical use.\u003c\/p\u003e\n\u003cp\u003e• Not available for sale in the United States.\u003c\/p\u003e\n\u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, laboratory clothing).\u003c\/p\u003e\n\u003cp\u003e• Handle positive control and extraction procedure in a biosafety cabinet; follow BSL‑2 laboratory safety guidelines.\u003c\/p\u003e\n\u003cp\u003e• Use nuclease-free consumables and reagents. Avoid cross-contamination between samples, controls and reagents.\u003c\/p\u003e\n\u003cp\u003e• Thaw kit components at 2–8 °C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n\u003cp\u003e• If VIC internal control signal is inhibited, repeat sample preparation or remove inhibitors and re-run the assay.\u003c\/p\u003e\n\u003cp\u003e• Store kit components according to label and check expiration date; items recommended at ≤ -20 °C should be stored within ±10 °C (–30 °C to –10 °C) per USP guidance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca href=\"#\" class=\"shentek-btn\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1506745.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1506745.pdf\" class=\"shentek-download-link\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay limit of detection (LOD)?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eThe reported limit of detection for the SHENTEK® PCV-2 Detection Kit is 50 copies per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which sample types are supported?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eRecommended sample types include cell culture (100–200 µL, ≤10^7 cells), bovine serum and similar biological matrices. Use the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for sample preparation.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret an inhibited internal control (VIC) signal?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eIf the VIC internal control is inhibited (reduced or absent VIC signal), this indicates potential PCR inhibitors or extraction failure. Repeat sample preparation, consider further purification to remove inhibitors, and re-run the assay.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should kit components be stored and what is shelf life?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore components as indicated on the labels, generally at -20 °C. Kit components are stable for up to 24 months; always check the expiration date on the product labels.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg fill=\"currentColor\" viewbox=\"0 0 20 20\" class=\"shentek-notice-icon\"\u003e\n \u003cpath clip-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" fill-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eNot sold in USA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45189096407221,"sku":"1506745","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Porcine_Circovirus_PCV-2_Detection_Kit.jpg?v=1742287945"},{"product_id":"skygen-chlamydia-spp-universal-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia spp.Universal Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11300 and is now updated to BF-34377554 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11300.pdf\"\u003e\u003cstrong\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11300.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097646261,"sku":"BF-34377554","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d57509db-6b33-4ab9-8fa0-48ac7597d6d9.jpg?v=1774176078"},{"product_id":"skygen-chlamydia-muridarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia muridarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia muridarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11340 and is now updated to BF-59544059 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11340.pdf\"\u003e\u003cstrong\u003eChlamydia muridarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11340.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097711797,"sku":"BF-59544059","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_856da674-f3d3-403b-a937-de0fbb07fe22.jpg?v=1774204110"},{"product_id":"skygen-chlamydia-caviae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia caviae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia caviae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11350 and is now updated to BF-43373408 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11350.pdf\"\u003e\u003cstrong\u003eChlamydia caviae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11350.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097810101,"sku":"BF-43373408","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_801f5bde-c74e-4130-bb25-0904a40b2d6e.jpg?v=1774157739"},{"product_id":"skygen-edwardsiella-spp-probe-realtime-pcr-kit-without-internal-control","title":"Broad Bean Wilt Virus I (BBWV I) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEdwardsiella spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-14600 and is now updated to BF-31177410 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-14600.pdf\"\u003e\u003cstrong\u003eEdwardsiella spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-14600.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281117798581,"sku":"BF-31177410","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bb771a40-be71-46dd-9018-06111efe2c76.jpg?v=1774172665"},{"product_id":"skygen-vibrio-spp-probe-realtime-pcr-kit-without-internal-control","title":"Brome Mosaic Virus (BMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eVibrio spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-14800 and is now updated to BF-73924451 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-14800.pdf\"\u003e\u003cstrong\u003eVibrio spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-14800.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281118519477,"sku":"BF-73924451","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_fec299a3-225f-4a22-a797-f4e868a24df6.jpg?v=1774173349"},{"product_id":"skygen-cheilospirura-hamulosa-probe-qpcr-kit-without-internal-control","title":"Hop Mosaic Virus (HpMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCheilospirura hamulosa Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26300 and is now updated to BF-94415446 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26300.pdf\"\u003e\u003cstrong\u003eCheilospirura hamulosa Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26300.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281150042293,"sku":"BF-94415446","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ff3b49ff-e8e4-459d-9729-1d5328985a60.jpg?v=1774190086"},{"product_id":"skygen-aspergillus-campestris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Aspergillus campestris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eAspergillus campestris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26780 and is now updated to BF-47481291 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26780.pdf\"\u003e\u003cstrong\u003eAspergillus campestris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26780.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151287477,"sku":"BF-47481291","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0226ce1c-921c-4fc0-829f-8d02d108d827.jpg?v=1774190208"},{"product_id":"skygen-cryptosporidium-galli-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium galli Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium galli Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26811 and is now updated to BF-66087926 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26811.pdf\"\u003e\u003cstrong\u003eCryptosporidium galli Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26811.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151910069,"sku":"BF-66087926","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0dacbbc6-fad2-40fd-90cd-32262c9bb1a9.jpg?v=1774186735"},{"product_id":"skygen-cryptosporidium-hominis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium hominis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26812 and is now updated to BF-76159985 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26812.pdf\"\u003e\u003cstrong\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26812.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152008373,"sku":"BF-76159985","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_57c29d05-f6fd-4774-9ab4-93002125db01.jpg?v=1774191062"},{"product_id":"skygen-cryptosporidium-molnari-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium molnari Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26814 and is now updated to BF-26550785 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26814.pdf\"\u003e\u003cstrong\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26814.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152041141,"sku":"BF-26550785","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b81128eb-9cce-4ccd-9f38-df246ccb93fa.jpg?v=1774160359"},{"product_id":"skygen-cryptosporidium-meleagridis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium meleagridis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26815 and is now updated to BF-60951899 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26815.pdf\"\u003e\u003cstrong\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26815.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152073909,"sku":"BF-60951899","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4d6e1dfc-516b-4784-bab8-0a2514d20233.jpg?v=1774177384"},{"product_id":"skygen-cryptosporidium-muris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium muris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium muris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26816 and is now updated to BF-33047002 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26816.pdf\"\u003e\u003cstrong\u003eCryptosporidium muris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26816.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152106677,"sku":"BF-33047002","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bfbcfe8a-2f8d-48ee-ae51-212d38b61faf.jpg?v=1774202367"},{"product_id":"skygen-cryptosporidium-nasorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium nasorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26817 and is now updated to BF-79876578 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26817.pdf\"\u003e\u003cstrong\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26817.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152139445,"sku":"BF-79876578","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_c17b4c37-67b8-4a1e-b8af-8877a07ce02c.jpg?v=1774192338"},{"product_id":"skygen-cryptosporidium-occultus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium occultus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26818 and is now updated to BF-83455011 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26818.pdf\"\u003e\u003cstrong\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26818.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152172213,"sku":"BF-83455011","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4e7d04d4-9142-4465-8f00-690b68a2fe2f.jpg?v=1774201908"},{"product_id":"skygen-cryptosporidium-ryanae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ryanae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26819 and is now updated to BF-61866087 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26819.pdf\"\u003e\u003cstrong\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26819.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152204981,"sku":"BF-61866087","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8d59fbe0-0b16-4251-ba27-c36dda80c31e.jpg?v=1774206225"},{"product_id":"skygen-cryptosporidium-suis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium suis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium suis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26821 and is now updated to BF-19266672 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26821.pdf\"\u003e\u003cstrong\u003eCryptosporidium suis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26821.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152303285,"sku":"BF-19266672","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d7482826-c68f-4b4e-87e2-a698eb209f6f.jpg?v=1774201916"},{"product_id":"skygen-cryptosporidium-scophthalmi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scophthalmi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26822 and is now updated to BF-94766909 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26822.pdf\"\u003e\u003cstrong\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26822.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152336053,"sku":"BF-94766909","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_61cc6c72-cb76-4a84-8f36-5b1f7a1103db.jpg?v=1774177268"},{"product_id":"skygen-cryptosporidium-saurophilum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium saurophilum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26823 and is now updated to BF-46075436 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26823.pdf\"\u003e\u003cstrong\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26823.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152368821,"sku":"BF-46075436","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e47a134c-3ffb-48db-838c-75102c21767c.jpg?v=1774206816"},{"product_id":"skygen-cryptosporidium-scrofarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scrofarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26824 and is now updated to BF-91078992 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26824.pdf\"\u003e\u003cstrong\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26824.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152401589,"sku":"BF-91078992","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ccebb0e2-16d8-4627-96c1-ad2d2c35701d.jpg?v=1774200598"},{"product_id":"skygen-cryptosporidium-ubiquitum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ubiquitum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26825 and is now updated to BF-22799761 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26825.pdf\"\u003e\u003cstrong\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26825.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152434357,"sku":"BF-22799761","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_59b61532-0e3b-4f22-b1cc-33fe450bbd97.jpg?v=1774191859"},{"product_id":"skygen-cryptosporidium-varaii-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium varaii Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26826 and is now updated to BF-20436497 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26826.pdf\"\u003e\u003cstrong\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26826.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152467125,"sku":"BF-20436497","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_90a4f4a7-c3e5-4bdb-8a21-742ef231a111.jpg?v=1774191497"},{"product_id":"skygen-cryptosporidium-viatorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium viatorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26827 and is now updated to BF-51393006 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26827.pdf\"\u003e\u003cstrong\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26827.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152696501,"sku":"BF-51393006","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_00593ebd-f248-45a2-9826-dd3e74f20d53.jpg?v=1774205906"},{"product_id":"skygen-cryptosporidium-wrairi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium wrairi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26828 and is now updated to BF-42857399 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26828.pdf\"\u003e\u003cstrong\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26828.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152794805,"sku":"BF-42857399","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_aa5a97d3-4f64-4c17-8fc4-bab8947c969b.jpg?v=1774171625"},{"product_id":"skygen-cryptosporidium-andersoni-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium andersoni Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26850 and is now updated to BF-56975258 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26850.pdf\"\u003e\u003cstrong\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26850.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153024181,"sku":"BF-56975258","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6cc007a5-0583-4244-997d-9fc1d510e6e2.jpg?v=1774204766"},{"product_id":"skygen-cryptosporidium-bovis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium bovis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26860 and is now updated to BF-59618136 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26860.pdf\"\u003e\u003cstrong\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26860.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153089717,"sku":"BF-59618136","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_769c899e-1b3d-45c4-af3e-7be8b290204e.jpg?v=1774189072"},{"product_id":"skygen-cryptosporidium-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26870 and is now updated to BF-53887329 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26870.pdf\"\u003e\u003cstrong\u003eCryptosporidium canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26870.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153188021,"sku":"BF-53887329","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_50324e85-c3cf-44c6-85d4-4aa1c0906d50.jpg?v=1774189063"},{"product_id":"skygen-cryptosporidium-felis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium felis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium felis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26880 and is now updated to BF-39439124 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26880.pdf\"\u003e\u003cstrong\u003eCryptosporidium felis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26880.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153220789,"sku":"BF-39439124","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_da2b8561-2924-43d9-8c04-d20f6aa0ac11.jpg?v=1774192285"},{"product_id":"skygen-cryptosporidium-fayeri-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium fayeri Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26890 and is now updated to BF-60900951 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26890.pdf\"\u003e\u003cstrong\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26890.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153253557,"sku":"BF-60900951","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_930ae5ab-4847-4abc-8cd5-ae507e7a812b.jpg?v=1774193639"},{"product_id":"skygen-schistosoma-spp-probe-realtime-pcr-kit-without-internal-control","title":"Pelargonium Flower Break Virus (PFBMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSchistosoma spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-32000 and is now updated to BF-74662901 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-32000.pdf\"\u003e\u003cstrong\u003eSchistosoma spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-32000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282314158261,"sku":"BF-74662901","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bcd232be-832f-4d8b-ab53-231bfb03ca1b.jpg?v=1774157331"},{"product_id":"skygen-klebsiella-spp-probe-realtime-pcr-kit-without-internal-control","title":"Potato Aucuba Mosaic Virus (PAMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eKlebsiella spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-36000 and is now updated to BF-56123008 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-36000.pdf\"\u003e\u003cstrong\u003eKlebsiella spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-36000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282324971701,"sku":"BF-56123008","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e4785a42-a8f7-44b8-b35c-d1e0c0f812e3.jpg?v=1774171866"},{"product_id":"skygen-cyprinid-herpesvirus-probe-qpcr-kit-without-internal-control","title":"Potato Black Ringspot Virus (PVRSV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCyprinid Herpesvirus Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-36100 and is now updated to BF-81973139 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-36100.pdf\"\u003e\u003cstrong\u003eCyprinid Herpesvirus Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-36100.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282325430453,"sku":"BF-81973139","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5199d577-4d5a-4b7f-9c30-67e13a1c7c94.jpg?v=1774186646"},{"product_id":"skygen-hepatitis-b-virus-h-probe-qpcr-kit-without-internal-control","title":"Potato Virus X (PVX) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHepatitis B Virus H Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-37000 and is now updated to BF-13609514 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-37000.pdf\"\u003e\u003cstrong\u003eHepatitis B Virus H Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-37000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282326216885,"sku":"BF-13609514","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_1a411391-2f33-47c7-aeb4-6fd78334b9b8.jpg?v=1774174472"},{"product_id":"skygen-riemerella-anatipestifer-probe-qpcr-kit-without-internal-control","title":"Rice Yellow Mottle Virus (RYMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eRiemerella anatipestifer Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-42800 and is now updated to BF-41369941 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-42800.pdf\"\u003e\u003cstrong\u003eRiemerella anatipestifer Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-42800.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282333851829,"sku":"BF-41369941","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b4865b71-fe3d-4ab3-951b-a26422555ea7.jpg?v=1774204610"},{"product_id":"skygen-avian-pneumovirus-probe-qrt-pcr-kit-without-internal-control","title":"Avian Pneumovirus Probe qRT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eAvian Pneumovirus Probe qRT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-43201 and is now updated to BF-43647403 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-43201.pdf\"\u003e\u003cstrong\u003eAvian Pneumovirus Probe qRT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-43201.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282334376117,"sku":"BF-43647403","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6b2bff69-1f10-4f60-944a-f76ba155f3e4.jpg?v=1774187086"},{"product_id":"skygen-subulura-brumpti-probe-qpcr-kit-without-internal-control","title":"Soil-borne Wheat Mosaic Virus (SbWMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSubulura brumpti Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-43300 and is now updated to BF-89216172 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-43300.pdf\"\u003e\u003cstrong\u003eSubulura brumpti Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-43300.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282334408885,"sku":"BF-89216172","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_09c81af4-a70b-45fa-92ec-01399b0bf2d1.jpg?v=1774160236"},{"product_id":"skygen-peptostreptococcus-spp-probe-realtime-pcr-kit-without-internal-control","title":"Southern Bean Mosaic Virus (SBMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003ePeptostreptococcus spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-43500 and is now updated to BF-62425901 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-43500.pdf\"\u003e\u003cstrong\u003ePeptostreptococcus spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-43500.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282334572725,"sku":"BF-62425901","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e8848efa-a492-4852-8195-99f149f2f884.jpg?v=1774167098"},{"product_id":"skygen-saprolegnia-parasitica-probe-qpcr-kit-without-internal-control","title":"Soybean Mosaic Virus (SMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSaprolegnia parasitica Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-43700 and is now updated to BF-82641466 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-43700.pdf\"\u003e\u003cstrong\u003eSaprolegnia parasitica Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-43700.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282334867637,"sku":"BF-82641466","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ee445b1a-1b48-49f7-b1a1-2f2fc837d339.jpg?v=1774156579"},{"product_id":"skygen-trichinella-spp-probe-realtime-pcr-kit-without-internal-control","title":"Sugarcane Streak Virus (SSV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eTrichinella spp. Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-44700 and is now updated to BF-65209020 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-44700.pdf\"\u003e\u003cstrong\u003eTrichinella spp. Probe Realtime PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-44700.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282336768181,"sku":"BF-65209020","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0b5a8bc4-6489-4893-bc7f-a3eedac146c4.jpg?v=1774171608"},{"product_id":"skygen-singapore-grouper-iridovirus-probe-qpcr-kit-without-internal-control","title":"Tobacco Necrosis Virus (TNV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSingapore Grouper Iridovirus Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-45500 and is now updated to BF-29420067 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-45500.pdf\"\u003e\u003cstrong\u003eSingapore Grouper Iridovirus Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-45500.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282337685685,"sku":"BF-29420067","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_f18d0eb0-53c2-4494-9c3f-ef9d3e9f6094.jpg?v=1774156608"},{"product_id":"skygen-barmah-forest-virus-probe-qrt-pcr-kit-without-internal-control","title":"Tomato Bushy Stunt Virus (TBSV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBarmah Forest Virus Probe qRT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-46000 and is now updated to BF-72776654 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-46000.pdf\"\u003e\u003cstrong\u003eBarmah Forest Virus Probe qRT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-46000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282338799797,"sku":"BF-72776654","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0b434041-d220-4e7e-803d-952ad73880f2.jpg?v=1774163899"},{"product_id":"skygen-bk-virus-probe-qpcr-kit-without-internal-control","title":"Tomato Mosaic Virus (ToMV) Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBK Virus Probe qPCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-46200 and is now updated to BF-53935333 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-46200.pdf\"\u003e\u003cstrong\u003eBK Virus Probe qPCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-46200.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282338865333,"sku":"BF-53935333","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_7b84b77d-971a-4056-9a5b-940027fcc22d.jpg?v=1774163879"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00001_58d6c748-925d-4859-acd6-24a40c2b342b.jpg?v=1776146838","url":"https:\/\/biofargo.com\/collections\/probe-rt-qpcr-kits.oembed?page=122","provider":"Biofargo","version":"1.0","type":"link"}