{"title":"Primary Cells","description":"","products":[{"product_id":"human-primary-kidney-fibroblasts","title":"Human Primary Kidney Fibroblasts","description":"\u003ch2\u003eDescription\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Kidney Fibroblasts from Cell Biologics are isolated from human kidney tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Kidney Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics.pdf?v=1744796071\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6016.pdf?v=1744796091\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6016\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC7919722\/\"\u003eJ Clin Invest. 2021 Mar 1; 131(5): e143645.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.science.org\/doi\/10.1126\/scitranslmed.abj2681?url_ver=Z39.88-2003\u0026amp;rfr_id=ori:rid:crossref.org\u0026amp;rfr_dat=cr_pub%20%200pubmed\"\u003e\u003cstrong\u003eSci Transl Med. 2022 Aug 17; 14(658): eabj2681.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.nature.com\/articles\/nature24676\"\u003e\u003cstrong\u003eNature. 2017 Dec 7; 552(7683): 110–115.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45304000118965,"sku":"H-6016","price":711.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45304000151733,"sku":"H-6017","price":742.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45304000184501,"sku":"H-6018","price":773.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fb1-500x500_841eb053-1bbb-4f14-9c06-0d3b2ae897b0.jpg?v=1778251433"},{"product_id":"c57bl-6-mouse-bone-marrow-macrophages","title":"C57BL\/6 Mouse Bone Marrow Macrophages","description":"\u003ch2\u003eDescription \u003c\/h2\u003e\n\u003cp\u003eC57BL\/6 Mouse Bone Marrow Macrophages from Cell Biologics are isolated from tibias and femurs of pathogen-free laboratory C57BL\/6 mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3x106 cells per ml. C57BL\/6 Mouse Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4\/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Cells can not be cultured indefinitely and can not be passaged. \u003c\/p\u003e\n\u003ch3\u003eSuggested Medium \u003c\/h3\u003e\n\u003cp\u003eM3368 Complete Macrophage Medium w\/ Kit (500 ml) \u003c\/p\u003e\n\u003ch2\u003ePrimary Macrophage Culture Protocol\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMedium Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M3368) to seed cells and when changing media.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCoating of Cell Culture Plates or Dishes Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 2 min, then aspirate the excess solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eCell Recovery from Cryovial\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThaw the cells quickly (\u0026lt;2 min) in a 37°C water bath until just prior to complete thawing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWipe the outside of the vial with 70% ethanol.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse 1 ml Pipette Tip to resuspend cells and transfer cells to a 15 ml sterile tube.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd 5 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M3368, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCentrifuge the cells at 200 x g at 4°C for 5 min.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCarefully remove the supernatant and resuspend in 7 mL pre-warmed Maintenance Medium (Catalog No. M3368, Cell Biologics).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eCarefully pour cell suspension into the cell culture plate or dish.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eRecommended Cell Seeding\u003c\/strong\u003e: 0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macroPlace a plate in a humidified, 5%-CO2 incubator at 37°C until experiments.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eChange fresh cell culture medium every 24-48 hours. Cells should be checked daily under a microscope to verify appropriate cell morphology.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eProceed with experiment assays.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: \u003c\/strong\u003ePlease send us the cell images (\u0026gt;90% confluence) if you have any question or problem with cultured cells.\u003c\/p\u003e\n\u003ch2\u003eStorage \u003c\/h2\u003e\n\u003cp\u003eCryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer. \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1508119223.pdf?v=1744797519\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. Warranty and Liability \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8263005\/\"\u003e\u003cstrong\u003emBio. 2021 May-Jun; 12(3): e01227-21.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8769200\/\"\u003e\u003cstrong\u003emSphere. 2022 Jan-Feb; 7(1): e00934-21.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial 3×10^6","offer_id":45304012931253,"sku":"C57-6030","price":598.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_macrophages.webp?v=1751472361"},{"product_id":"human-primary-aortic-endothelial-cells","title":"Human Primary Aortic Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Aortic Endothelial Cells from Cell Biologics are isolated from human aorta tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Aortic Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Aortic Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Primary_Endothelial_Cell_Culture_Protocol.pdf?v=1745372502\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6052.pdf?v=1745372502\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6052\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_0c8dd8f8-c565-4c21-bd6d-206c23860428.pdf?v=1745372502\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8461814\/\"\u003eAm J Physiol Cell Physiol. 2021 Sep 1; 321(3): C535–C548.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45317879857333,"sku":"H-6052","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45317879890101,"sku":"H-6052-T2","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45317879922869,"sku":"H-6052-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HLMVEC-MT-cadh1_copy-500x500.jpg?v=1778249605"},{"product_id":"human-primary-bladder-microvascular-endothelial-cells","title":"Human Primary Bladder Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Bladder Microvascular Endothelial Cells from Cell Biologics are isolated from human bladder tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Bladder Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Bladder Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_6a4bec84-c06c-48b6-9231-d05ed43f45c6.pdf?v=1745378136\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6214.pdf?v=1745378136\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6214\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Bladder_Microvascular_Endothelial_Cells.pdf?v=1745378136\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318090064053,"sku":"H-6214","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318090096821,"sku":"H-6214-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318090129589,"sku":"H-6214-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500_80018c6c-fe09-4104-8333-b5bd31ac0fc2.jpg?v=1778252042"},{"product_id":"human-primary-brain-microvascular-endothelial-cells","title":"Human Primary Brain Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Brain Microvascular Endothelial Cells from Cell Biologics are isolated from human brain tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Brain Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Brain Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_a5f69946-a021-4296-98ee-1ef0654e40e3.pdf?v=1745378834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6023.pdf?v=1745378834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6023\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Brain_Microvascular_Endothelial_Cells.pdf?v=1745378834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9485157\/\"\u003eNat Commun. 2022; 13: 5494. Published online 2022 Sep 19. doi: 10.1038\/s41467-022-33235-7\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5658400\/\"\u003eNat Commun. 2017; 8: 1145.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9103166\/\"\u003eNat Cardiovasc Res. Author manuscript; available in PMC 2022 Oct 13.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318092456117,"sku":"H-6023","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318092488885,"sku":"H-6023-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318092521653,"sku":"H-6023-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_edb36881-c12c-4871-b792-f726cf368cbe.jpg?v=1778252086"},{"product_id":"human-primary-cardiac-microvascular-endothelial-cells","title":"Human Primary Cardiac Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Cardiac Microvascular Endothelial Cells from Cell Biologics are isolated from human heart tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Cardiac Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Cardiac Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6024.pdf?v=1745379937\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6024\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_f587e32b-e28c-4188-900c-0ad92d0e6b1e.pdf?v=1745379937\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Cardiac_Microvascular_Endothelial_Cells.pdf?v=1745379937\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9496506\/\"\u003eBiomedicines. 2022 Sep; 10(9): 2216. Published online 2022 Sep 7. doi: 10.3390\/biomedicines10092216\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318099599541,"sku":"H-6024","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318099632309,"sku":"H-6024-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318099665077,"sku":"H-6024-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rat_EC._Tube_formation-500x500.jpg?v=1778251698"},{"product_id":"human-primary-carotid-artery-endothelial-cells","title":"Human Primary Carotid Artery Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Artery Endothelial Cells from Cell Biologics are isolated from human artery tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Artery Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_ebfa1324-b391-4d7d-81a4-b868bfbb4a26.pdf?v=1745385386\" type=\"application\/pdf\" target=\"_blank\"\u003eMSDS.CellBiologics   \u003c\/a\u003e  \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Carotid_Artery_Endothelial_Cells.pdf?v=1745385386\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6008.pdf?v=1745385387\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6008\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318138298549,"sku":"H-6008","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318138331317,"sku":"H-6008-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318138364085,"sku":"H-6008-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_b971b877-f6e7-4bbe-aff7-fcd09d5d0637.jpg?v=1778252145"},{"product_id":"human-primary-colonic-microvascular-endothelial-cells","title":"Human Primary Colonic Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Colonic Microvascular Endothelial Cells from Cell Biologics are isolated from human colonic tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Colonic Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Colonic Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Colonic_Microvascular_Endothelial_Cells.pdf?v=1745385834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_56b1d77b-477e-4dd5-b33b-28e498bcae06.pdf?v=1745385834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6203.pdf?v=1745385834\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6203\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8551844\/\"\u003e\u003cstrong\u003eCell Mol Gastroenterol Hepatol. 2021; 12(5): 1719–1741. Published online 2021 Jul 17. doi: 10.1016\/j.jcmgh.2021.07.004\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318143082677,"sku":"H-6203","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318143115445,"sku":"H-6203-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318143148213,"sku":"H-6203-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_6379618b-f067-4020-945c-8a87e493d770.jpg?v=1778252098"},{"product_id":"human-primary-coronary-artery-endothelial-cells","title":"Human Primary Coronary Artery Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Coronary Artery Endothelial Cells from Cell Biologics are isolated from human coronary artery tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Coronary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Coronary Artery Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Coronary_Artery_Endothelial_Cells.pdf?v=1745386217\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_dbc6813b-d558-426f-b5f8-fe2df5e9ea8d.pdf?v=1745386218\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6093.pdf?v=1745386217\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6093\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318159925429,"sku":"H-6093","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318159958197,"sku":"H-6093-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318159990965,"sku":"H-6093-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500_0ec80ca6-4497-45d0-bb9d-073f6b20af55.jpg?v=1778252046"},{"product_id":"human-primary-dermal-lymphatic-endothelial-cells","title":"Human Primary Dermal Lymphatic Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Dermal Lymphatic Endothelial Cells from Cell Biologics are isolated from human skin tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Dermal Lymphatic Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Dermal Lymphatic Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Dermal_Lymphatic_Endothelial_Cells.pdf?v=1745387522\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_14352b18-7035-4c9f-b4d2-4ed70ce4833a.pdf?v=1745387522\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6064L.pdf?v=1745387522\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6064L\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4919545\/\"\u003e\u003cstrong\u003eNat Commun. 2016; 7: 12021.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318183780533,"sku":"H-6064L","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318183813301,"sku":"H-6064L-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318183846069,"sku":"H-6064L-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/LYVE1.Image_2466_copy-500x500.jpg?v=1778252916"},{"product_id":"human-primary-dermal-microvascular-endothelial-cells","title":"Human Primary Dermal Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Dermal Microvascular Endothelial Cells from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Dermal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Dermal Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Dermal_Microvascular_Endothelial_Cells.pdf?v=1745387814\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_22cc040b-c698-4a58-abc1-b15014355336.pdf?v=1745387814\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6064.pdf?v=1745387814\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6064\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3252978\/\"\u003e\u003cstrong\u003eBr J Pharmacol. 2012 Jan; 165(1): 208–222.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318184861877,"sku":"H-6064","price":752.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318184894645,"sku":"H-6064-T25","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318184927413,"sku":"H-6064-T75","price":917.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500_901e13b6-b208-47d0-aef7-382f2e5a0079.jpg?v=1778252062"},{"product_id":"human-primary-intestinal-mesenteric-vascular-endothelial-cells","title":"Human Primary Intestinal Mesenteric Vascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Intestinal Mesenteric Vascular Endothelial Cells from Cell Biologics are isolated from human intestinal mesenteric vascular tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Intestinal Mesenteric Vascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Intestinal Mesenteric Vascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Intestinal_Mesenteric_Vascular_Endothelial_Cells.pdf?v=1745388061\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6055.pdf?v=1745388061\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6055\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_d5a94051-f0da-4170-bf23-4f488b9b5ef0.pdf?v=1745388061\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318187581621,"sku":"H-6055","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318187614389,"sku":"H-6055-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318187647157,"sku":"H-6055-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1.hEC.anti-VE-cadherin_copy-500x500.jpg?v=1778252937"},{"product_id":"human-primary-kidney-glomerular-endothelial-cells","title":"Human Primary Kidney Glomerular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from human kidney tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Kidney Glomerular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Kidney Glomerular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Kidney_Glomerular_Endothelial_Cells.pdf?v=1745391997\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_c1e24ee6-e429-4104-a6eb-99d1cb4f9deb.pdf?v=1745391998\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6014G.pdf?v=1745391998\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6014G\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10690916\/\"\u003e\u003cstrong\u003eHaematologica. 2023 Dec 1; 108(12): 3449–3453.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318331039925,"sku":"H-6014G","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318331072693,"sku":"H-6014G-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318331105461,"sku":"H-6014G-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_ea0a469b-88cc-49e3-83bc-4f9722038e48.jpg?v=1778252102"},{"product_id":"human-primary-liver-sinusoidal-microvascular-endothelial-cells","title":"Human Primary Liver Sinusoidal Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Liver Sinusoidal Microvascular Endothelial Cells from Cell Biologics are isolated from human liver tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Liver Sinusoidal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Liver Sinusoidal Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_e6f8f52d-ad9e-4a5f-8437-fbc15e55bd01.pdf?v=1745392337\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6017.pdf?v=1745392337\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6017\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318341329077,"sku":"H-6017","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318341361845,"sku":"H-6017-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318341394613,"sku":"H-6017-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HLMVEC-MT-cadh1_copy-500x500_b7f2bda1-e1a1-4ec5-8674-ed17079991d6.jpg?v=1778249609"},{"product_id":"human-primary-lung-microvascular-endothelial-cells","title":"Human Primary Lung Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from human lung tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Lung Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_ff19310e-da3b-447f-9be9-1aaa1981cb0a.pdf?v=1745393521\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Lung_Microvascular_Endothelial_Cells.pdf?v=1745393521\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003ePrimary Endothelial Cell Culture Protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6011.pdf?v=1745393522\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6011\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10642369\/\"\u003e\u003cstrong\u003eCell. 2023 Nov 9; 186(23): 5135–5150.e28.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6650612\/#\"\u003e\u003cstrong\u003eSci Rep. 2019; 9: 10634.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Via","offer_id":45318388711605,"sku":"H-6011","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318388744373,"sku":"H-6011-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318388777141,"sku":"H-6011-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_55cb7af8-6e32-49d9-9cb3-ed7c41e1f84b.jpg?v=1778252150"},{"product_id":"human-primary-lymphatic-endothelial-cells","title":"Human Primary Lymphatic Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Lymphatic Endothelial Cells from Cell Biologics are isolated from human lymph node tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Lymphatic Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Lymphatic Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6092.pdf?v=1745396418\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6092\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_d0c3d970-53db-40bb-8730-a9e13d4f7373.pdf?v=1745396418\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8593614\/\"\u003eiScience. 2021 Nov 19; 24(11): 103386.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/entrez\/eutils\/elink.fcgi?dbfrom=pubmed\u0026amp;retmode=ref\u0026amp;cmd=prlinks\u0026amp;id=37205636\"\u003eCancer Res. 2023 Jul 5; 83(13): 2105–2122.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6125167\/#\"\u003eJ Immunol. Author manuscript; available in PMC 2019 Sep 15.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318476497077,"sku":"H-6092","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318476529845,"sku":"H-6092-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318476562613,"sku":"H-6094-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/LYVE1.Image_2466_copy-500x500_4b515784-dd1e-4aaf-8f63-9e236020c294.jpg?v=1778252925"},{"product_id":"human-primary-mammary-microvascular-endothelial-cells","title":"Human Primary Mammary Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Mammary Microvascular Endothelial Cells from Cell Biologics are isolated from human breast tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Mammary Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Mammary Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_0bc14715-c3b0-48ef-8f59-7d069f4e4455.pdf?v=1745396632\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6020.pdf?v=1745396632\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6020\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318479380661,"sku":"H-6020","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318479413429,"sku":"H-6020-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318479446197,"sku":"H-6020-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_149149bc-4bf3-4292-8d99-9e655e9791b0.jpg?v=1778252094"},{"product_id":"human-primary-ovarian-microvascular-endothelial-cells","title":"Human Primary Ovarian Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Ovarian Microvascular Endothelial Cells from Cell Biologics are isolated from human ovarian tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Ovarian Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Ovarian Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_21fa66f3-3312-4418-84bd-62145245e203.pdf?v=1745396977\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6090.pdf?v=1745396977\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6090\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318484787381,"sku":"H-6090","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318484820149,"sku":"H-6090-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318484852917,"sku":"H-6090-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500.jpg?v=1778252020"},{"product_id":"human-primary-pancreatic-microvascular-endothelial-cells","title":"Human Primary Pancreatic Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Pancreatic Microvascular Endothelial Cells from Cell Biologics are isolated from human pancreatic tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Pancreatic Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Pancreatic Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_9cc912c2-d58c-4118-b52a-fc3c3572d51c.pdf?v=1745397222\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6206.pdf?v=1745397222\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6206\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318494290101,"sku":"H-6206","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318494322869,"sku":"H-6206-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318494355637,"sku":"H-6206-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1.hEC.anti-VE-cadherin_copy-500x500_0fe9124e-17d6-4856-9780-26c2b7bb3d3d.jpg?v=1778252945"},{"product_id":"human-primary-prostate-microvascular-endothelial-cells","title":"Human Primary Prostate Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Prostate Microvascular Endothelial Cells from Cell Biologics are isolated from human prostate tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Prostate Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Prostate Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_97631df1-9680-4009-9578-a26ff6d613e9.pdf?v=1745397495\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6027.pdf?v=1745397495\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6027\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318499008693,"sku":"H-6027","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318499041461,"sku":"H-6027-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318499074229,"sku":"H-6027-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_e76c5d8a-8224-4ffe-82ac-c4ac0279add5.jpg?v=1778252166"},{"product_id":"human-primary-pulmonary-artery-endothelial-cells","title":"Human Primary Pulmonary Artery Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Pulmonary Artery Endothelial Cells from Cell Biologics are isolated from human pulmonary artery and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Pulmonary Artery Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Pulmonary Artery Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_f9326fd6-7382-4fe1-b8f7-5a22906d1050.pdf?v=1745397833\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6059.pdf?v=1745397833\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6059\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318506152117,"sku":"H-6059","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318506184885,"sku":"H-6059-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318506217653,"sku":"H-6059-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2.mEC.LDL_Dil_copy-500x500.jpg?v=1778250649"},{"product_id":"human-primary-pulmonary-vein-endothelial-cells","title":"Human Primary Pulmonary Vein Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Pulmonary Vein Endothelial Cells from Cell Biologics are isolated from human pulmonary vein and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Pulmonary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Pulmonary Vein Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1426266361.pdf?v=1745398289\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6060\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_d4c8020a-d109-4727-a5d0-8a6ee87f42a6.pdf?v=1745398289\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318528139445,"sku":"H-6060","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318528172213,"sku":"H-6060-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318528204981,"sku":"H-6060-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500_4f372f5c-5362-4cfb-8856-ac8fc1375f36.jpg?v=1778252029"},{"product_id":"human-primary-skeletal-muscle-microvascular-endothelial-cells","title":"Human Primary Skeletal Muscle Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Skeletal Muscular Microvascular Endothelial Cells from Cell Biologics are isolated from human skeletal muscle tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Skeletal Muscular Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Skeletal Muscular Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1426266401.pdf?v=1745398562\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6220\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_14f5eca2-35ab-4d98-bb7c-71faf27ef341.pdf?v=1745398562\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"T25 Flask","offer_id":45318529646773,"sku":"H-6220-T25","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318529679541,"sku":"H-6220-T75","price":917.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_347fec0f-7a08-4442-9ab6-013d8d488585.jpg?v=1778252090"},{"product_id":"human-primary-small-intestinal-microvascular-endothelial-cells","title":"Human Primary Small Intestinal Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Small Intestinal Microvascular Endothelial Cells from Cell Biologics are isolated from human small intestinal tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Small Intestinal Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Small Intestinal Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_2c47d1a4-c692-4b18-814e-4839ce71ff93.pdf?v=1745398899\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6054.pdf?v=1745398899\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6054\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318539083957,"sku":"H-6054","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318539116725,"sku":"H-6054-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318539149493,"sku":"H-6054-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_102_copy-500x500_1ce110f9-df3b-41e9-8bea-8a8c481127fc.jpg?v=1778252038"},{"product_id":"human-primary-spleen-microvascular-endothelial-cells","title":"Human Primary Spleen Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Spleen Endothelial Cells from Cell Biologics are isolated from human spleen tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Spleen Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Spleen Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1426266435.pdf?v=1745399203\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6057\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_798a9694-95de-47b0-9889-42a1c6bce286.pdf?v=1745399203\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318542524597,"sku":"H-6057","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318542557365,"sku":"H-6057-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318542590133,"sku":"H-6057-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_de19c941-7499-4e00-babd-3c01d59c96f3.jpg?v=1778252141"},{"product_id":"human-primary-thymus-microvascular-endothelial-cells","title":"Human Primary Thymus Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Thymus Endothelial Cells from Cell Biologics are isolated from human thymus tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Thymus Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Thymus Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1426266457.pdf?v=1745399406\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6208\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_c301378b-2b3a-4a0a-971b-f1ade113f2f1.pdf?v=1745399406\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318545375413,"sku":"H-6208","price":752.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318545408181,"sku":"H-6208-T25","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318545440949,"sku":"H-6208-T75","price":917.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_9297199e-ed3b-4962-ae4f-db720aef8573.jpg?v=1778252170"},{"product_id":"human-primary-thyroid-microvascular-endothelial-cells","title":"Human Primary Thyroid Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Thyroid Microvascular Endothelial Cells from Cell Biologics are isolated from human thyroid tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Thyroid Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Thyroid Microvascular Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Human_Primary_Thyroid_Microvascular_Endothelial_Cells.pdf?v=1745399779\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eEndothelial cell culture protocol\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6058.pdf?v=1745399780\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6058\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_508010a4-d120-42b2-a23e-c990c53d12f8.pdf?v=1745399781\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318549668021,"sku":"H-6058","price":752.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318549700789,"sku":"H-6058-T25","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318549733557,"sku":"H-6058-T75","price":917.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HLMVEC-MT_copy-500x500.jpg?v=1778249647"},{"product_id":"human-primary-umbilical-vein-endothelial-cells","title":"Human Primary Umbilical Vein Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells from Cell Biologics are isolated from human umbilical vein tissue. Human Primary Umbilical Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×106 cells per ml. \u003c\/p\u003e\n\u003ch2\u003eProduct Testing\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells from Cell Biologics display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen\u003c\/p\u003e\n\u003cp\u003e(-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_7c60589a-8fe7-4f68-971e-64e3789f85ac.pdf?v=1745400136\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6207.pdf?v=1745400135\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6207\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Although Human Primary Umbilical Vein Endothelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318556254389,"sku":"H-6207","price":629.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318556287157,"sku":"H-6207-T25","price":783.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500.jpg?v=1778252081"},{"product_id":"human-primary-vein-endothelial-cells","title":"Human Primary Vein Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Vein Endothelial Cells from Cell Biologics are isolated from human vein tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Vein Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31\/PECAM-1 (Catalog No. FAB3567P, R\u0026amp;D), VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. Human Primary Vein Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH1168 Complete Human Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_2708d280-d6a9-44a8-ae07-908345ba363b.pdf?v=1745400429\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6009.pdf?v=1745400429\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6009\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318565396661,"sku":"H-6009","price":835.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318565429429,"sku":"H-6009-T25","price":855.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318565462197,"sku":"H-6009-T75","price":989.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2.mEC.LDL_Dil_copy-500x500_bfc33391-b8f2-4c45-a335-4755fbd9a9d5.jpg?v=1778251072"},{"product_id":"human-primary-alveolar-epithelial-cells-1","title":"Human Primary Alveolar Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Alveolar Epithelial Cells from Cell Biologics are isolated from human lung tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Alveolar Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD),  E-Cadherin (Cat# SC-8426, Santa Cruz) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_48a57925-5d1b-4fe6-9bd7-233bdc378b82.pdf?v=1745401260\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6053_b627e18b-8add-4e4e-859c-0c54b2985841.pdf?v=1745401260\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6053\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10579267\/#\"\u003e\u003cstrong\u003eNat Commun. 2023; 14: 6506.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10236152\/#\"\u003e\u003cstrong\u003eCancer Immunol Res. 2023 Jun 2; 11(6): 792–809.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318587547829,"sku":"H-6053","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318587580597,"sku":"H-6053-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318587613365,"sku":"H-6053-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/010aEpiC.L-500x500_65e08063-f6c0-4051-8342-74aaf8285645.jpg?v=1778251947"},{"product_id":"human-primary-bladder-epithelial-cells","title":"Human Primary Bladder Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Bladder Epithelial Cells from Cell Biologics are isolated from human bladder tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Bladder Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_480e666f-c6ca-4e6c-ad8f-dceae79aae6d.pdf?v=1745401597\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6215.pdf?v=1745401598\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6215\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318594232501,"sku":"H-6215","price":680.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318594265269,"sku":"H-6215-T25","price":742.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318594298037,"sku":"H-6215-T75","price":897.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EpithelialCells_copy-500x500.jpg?v=1778250334"},{"product_id":"human-primary-bronchial-epithelial-cells","title":"Human Primary Bronchial Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Bronchial Epithelial Cells from Cell Biologics are isolated from human bronchial tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Bronchial Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_a0c25623-4692-4dfe-8341-1478d6667cd4.pdf?v=1745401979\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6033B.pdf?v=1745401980\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6033B\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7516381\/#\"\u003eAm J Physiol Lung Cell Mol Physiol. 2020 Oct 1; 319(4): L670–L674.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318607831221,"sku":"H-6033B","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318607863989,"sku":"H-6033B-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318607896757,"sku":"H-6033B-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/005aKd_EpiC.PNAB-500x500.jpg?v=1778252892"},{"product_id":"human-primary-colonic-epithelial-cells","title":"Human Primary Colonic Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Colonic Epithelial Cells from Cell Biologics are isolated from human colonic tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Colonic Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz),  or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_ef968dc8-b335-47a6-abe9-1e490d8bec5d.pdf?v=1745402368\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6047.pdf?v=1745402368\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6047\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10406808\/#\"\u003e\u003cstrong\u003eNat Commun. 2023; 14: 4737.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9894309\/#\"\u003e\u003cstrong\u003eCell Genom. 2022 Nov 9; 2(11): 100191.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318611992757,"sku":"H-6047","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318612025525,"sku":"H-6047-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318612058293,"sku":"H-6047-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/007AEpithelialCell.L-500x500_ebd977ff-b26a-498e-a427-269b99b3df95.jpg?v=1778252868"},{"product_id":"human-primary-corneal-epithelial-cells","title":"Human Primary Corneal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Corneal Epithelial Cells from Cell Biologics are isolated from human corneal tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Corneal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_c29a0a62-ed75-48c8-97b6-b79e8f15341b.pdf?v=1745402862\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6048.pdf?v=1745402862\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6048\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318614188213,"sku":"H-6048","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318614220981,"sku":"H-6048-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318614253749,"sku":"H-6048-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/014a-500x500_0a49e528-cc77-43bc-9bdd-fa0fc128c27d.jpg?v=1778250466"},{"product_id":"human-primary-dermal-epithelial-cells","title":"Human Primary Dermal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Dermal Epithelial Cells from Cell Biologics are isolated from human skin tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Dermal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_9a07a9c2-46c8-4370-9b51-0b1396298f23.pdf?v=1745403173\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6066.pdf?v=1745403173\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6066\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45318616907957,"sku":"H-6066","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45318616940725,"sku":"H-6066-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45318616973493,"sku":"H-6066-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/014a-500x500_ced3c315-692a-4c69-abb7-320f1ca693f5.jpg?v=1778250453"},{"product_id":"human-primary-esophageal-epithelial-cells","title":"Human Primary Esophageal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Esophageal Epithelial Cells from Cell Biologics are isolated from human esophageal tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Esophageal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz)  or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_5aa808fc-e532-4f9f-b6bb-c524d02a65dc.pdf?v=1745481975\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6046.pdf?v=1745481975\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6046\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10839056\/#\"\u003e\u003cstrong\u003eFront Immunol. 2024; 15: 1347259.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10406808\/#\"\u003e\u003cstrong\u003eNat Commun. 2023; 14: 4737.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322528260277,"sku":"H-6046","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322528293045,"sku":"H-6046-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322528325813,"sku":"H-6046-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/015aEpiC.L-500x500_87c9d38c-fb65-4d35-93dd-739e529a896c.jpg?v=1778250518"},{"product_id":"human-primary-gingival-epithelial-cells","title":"Human Primary Gingival Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Gingival Epithelial Cells from Cell Biologics are isolated from human gingival tissues and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Gingival Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_c0f0fa4e-d358-493e-aa18-c6fcc5387b65.pdf?v=1745483257\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6202.pdf?v=1745483257\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6202\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322532192437,"sku":"H-6202","price":680.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322532225205,"sku":"H-6202-T25","price":742.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322532257973,"sku":"H-6202-T75","price":897.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/001aEpiC.L-500x500_3ba21f7a-9cac-48fc-aaf6-e003f978435f.jpg?v=1778251646"},{"product_id":"human-primary-kidney-epithelial-cells","title":"Human Primary Kidney Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Kidney Epithelial Cells from Cell Biologics are isolated from human kidney tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Kidney Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_8e55baa1-38fe-439c-ad0e-34f961295c3b.pdf?v=1745483591\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6034.pdf?v=1745483591\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6034\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10406808\/#\"\u003eNat Commun. 2023; 14: 4737.\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322539008181,"sku":"H-6034","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322539040949,"sku":"H-6034-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322539073717,"sku":"H-6034-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/006aKd_EpiC.PNAB-500x500.jpg?v=1778249488"},{"product_id":"human-primary-mammary-epithelial-cells","title":"Human Primary Mammary Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Mammary Epithelial Cells from Cell Biologics are isolated from human breast tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Mammary Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_bb1ab3d5-cd6f-4d1a-9686-08ed8d734f55.pdf?v=1745484231\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6035.pdf?v=1745484231\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6035\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322559029429,"sku":"H-6035","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322559062197,"sku":"H-6035-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322559094965,"sku":"H-6035-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/015aEpiC.L-500x500_a7ecfde3-f6a2-4dbd-aa13-feb6c890a53e.jpg?v=1778250510"},{"product_id":"human-primary-oral-mucosal-epithelial-cells","title":"Human Primary Oral Mucosal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Mucosal Epithelial Cells from Cell Biologics are isolated from human mucosal tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Mucosal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch2\u003eSuggested Medium\u003c\/h2\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322566598837,"sku":"H-6234","price":701.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322566631605,"sku":"H-6234-T25","price":742.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322566664373,"sku":"H-6234-T75","price":897.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Image_287.jpgEpiC.Lung.AT1-500x500_f0c307d1-9ddb-4b41-bc98-e01522bb0b5b.jpg?v=1778272183"},{"product_id":"human-primary-liver-epithelial-cells-1","title":"Human Primary Liver Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Liver Epithelial Cells from Cell Biologics are isolated from human liver tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Liver Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_6f666bd7-1ae8-4a7c-8a1b-99e85065f2f9.pdf?v=1745485252\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6044_6955c1f8-1716-4358-8e04-48d469bc966d.pdf?v=1745485252\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6044\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8203650\/#\"\u003e\u003cstrong\u003eNat Commun. 2021; 12: 3615.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10097865\/#\"\u003e\u003cstrong\u003eNat Commun. 2023; 14: 2068.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322591207605,"sku":"H-6044","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322591240373,"sku":"H-6044-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322591273141,"sku":"H-6044-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/007AEpithelialCell.L-500x500.jpg?v=1778252846"},{"product_id":"human-primary-ovarian-epithelial-cells","title":"Human Primary Ovarian Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Ovarian Epithelial Cells from Cell Biologics are isolated from human ovarian tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Ovarian Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1465329799.pdf?v=1745485989\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6036\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_a455707e-3bb9-487b-8d75-e6e63327a1ff.pdf?v=1745485990\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6980601\/#\"\u003e\u003cstrong\u003ePLoS One. 2020; 15(1): e0228024.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9894309\/#\"\u003e\u003cstrong\u003eCell Genom. 2022 Nov 9; 2(11): 100191.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322605166773,"sku":"H-6036","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322605199541,"sku":"H-6036-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322605232309,"sku":"H-6036-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/010aEpiC.L-500x500_b9f3fca0-1d95-4c34-aad6-0b098dd24c0e.jpg?v=1778251921"},{"product_id":"human-primary-pancreatic-epithelial-cells","title":"Human Primary Pancreatic Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Pancreatic Epithelial Cells from Cell Biologics are isolated from human pancreatic tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Pancreatic Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz)  or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_4a9af6b4-2c59-436d-8de9-a6b09008f3ae.pdf?v=1745486241\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6037.pdf?v=1745486241\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6037\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9894309\/#\"\u003e\u003cstrong\u003eCell Genom. 2022 Nov 9; 2(11): 100191.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9496506\/#\"\u003e\u003cstrong\u003eBiomedicines. 2022 Sep; 10(9): 2216.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322609688757,"sku":"H-6037","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322609721525,"sku":"H-6037-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322609754293,"sku":"H-6037-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/015aEpiC.L-500x500_9e7fffc7-6a2c-4b04-a097-a1f83b79ffb2.jpg?v=1778250528"},{"product_id":"human-primary-prostate-epithelial-cells","title":"Human Primary Prostate Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Prostate Epithelial Cells from Cell Biologics are isolated from human prostate tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Prostate Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1465329819.pdf?v=1745486742\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6038\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_3babdb35-cbcc-4f0f-8bbe-17a52eb10d1e.pdf?v=1745486742\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322623975605,"sku":"H-6038","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322624008373,"sku":"H-6038-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322624041141,"sku":"H-6038-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EpithelialCells_copy-500x500_c6a59351-78f5-4db3-96d4-c206e11eed6a.jpg?v=1778250342"},{"product_id":"human-primary-proximal-tubular-epithelial-cells","title":"Human Primary Proximal Tubular Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Proximal Tubular Epithelial Cells from Cell Biologics are isolated from human proximal tubular tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Proximal Tubular Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_8f924d60-2772-4974-ab51-24ffa9393aec.pdf?v=1745488443\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6015.pdf?v=1745488442\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6015\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9894309\/#\"\u003e\u003cstrong\u003eCell Genom. 2022 Nov 9; 2(11): 100191.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10406808\/#\"\u003e\u003cstrong\u003eNat Commun. 2023; 14: 4737.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322655826101,"sku":"H-6015","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322655858869,"sku":"H-6015-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322655891637,"sku":"H-6015-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/006aKd_EpiC.PNAB-500x500_4ccaf365-71dc-42d6-8e75-c9b52843df4c.jpg?v=1778249501"},{"product_id":"human-primary-small-intestinal-epithelial-cells","title":"Human Primary Small Intestinal Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Small Intestinal Epithelial Cells from Cell Biologics are isolated from human small intestinal tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Small Intestinal Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD), E-Cadherin (Cat# SC-8426, Santa Cruz)  or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_8253d716-2aed-443a-8323-eb6e4d3359c7.pdf?v=1745488725\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6051.pdf?v=1745488725\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6051\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10453699\/#\"\u003e\u003cstrong\u003eCurr Issues Mol Biol. 2023 Aug; 45(8): 6743–6774.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7328038\/#\"\u003e\u003cstrong\u003eJ Lipid Res. 2020 Jul; 61(7): 1115–1127.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":45322659856565,"sku":"H-6051","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45322659889333,"sku":"H-6051-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45322659922101,"sku":"H-6051-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/007AEpithelialCell.L-500x500_88b946cd-b0f2-4601-8ccb-b62d03e612d8.jpg?v=1778252859"},{"product_id":"human-primary-spleen-epithelial-cells","title":"Human Primary Spleen Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Spleen Epithelial Cells from Cell Biologics are isolated from human spleen tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Spleen Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_680a588b-3636-490a-8237-d2ed7c6c62ab.pdf?v=1745825087\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6045.pdf?v=1745825087\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6045\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Forzen Vial","offer_id":45329527767221,"sku":"H-6045","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45329527799989,"sku":"H-6045-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45329527832757,"sku":"H-6045-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EpithelialCells_copy-500x500_1e2e563a-fa22-4b03-9fd7-a8855e9bb010.jpg?v=1778250348"},{"product_id":"human-primary-stomach-epithelial-cells","title":"Human Primary Stomach Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Stomach Epithelial Cells from Cell Biologics are isolated from human stomach tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Stomach Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_20dd442c-f1cb-411a-a5ee-229568747a1a.pdf?v=1745825426\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6039.pdf?v=1745825426\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6039\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10627204\/#\"\u003e\u003cstrong\u003eBiomedicine (Taipei). 2023; 13(2): 14–23.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8916167\/#\"\u003e\u003cstrong\u003eNature. Author manuscript; available in PMC 2022 Mar 11.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Forzen Vial","offer_id":45329542119605,"sku":"H-6039","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45329542152373,"sku":"H-6039-T25","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45329542185141,"sku":"H-6039-T75","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/010aEpiC.L-500x500_4b8b970d-46c0-4b2d-a114-6bb0cf6838c6.jpg?v=1778251925"},{"product_id":"human-primary-thymus-epithelial-cells","title":"Human Primary Thymus Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Thymus Epithelial Cells from Cell Biologics are isolated from human thymus tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Thymus Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1465329881.pdf?v=1745825793\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6209\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_9a5eba10-cfa3-4249-a2fe-1aa6fc406a35.pdf?v=1745825793\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Forzen Vial","offer_id":45329743610037,"sku":"H-6209","price":680.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45329743642805,"sku":"H-6209-T25","price":742.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45329743675573,"sku":"H-6209-T75","price":897.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EpithelialCells_copy-500x500_582a14d4-c818-455b-9b79-89c9f813094a.jpg?v=1778250368"},{"product_id":"human-primary-thyroid-epithelial-cells","title":"Human Primary Thyroid Epithelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Thyroid Epithelial Cells from Cell Biologics are isolated from human thyroid tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Thyroid Epithelial Cells from Cell Biologics are characterized by immunofluorescent staining with E-cadherin (Catalog No. 610182 from BD) or ZO-1 (Catalog No. 617300; from Life Technologies) antibody. These Cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eH6621 Complete Epithelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.CellBiologics_a132c0a9-4d7d-4d4e-a722-f97aeffcd6b5.pdf?v=1746671936\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS.CellBiologics\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/H-6040.pdf?v=1746671936\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6040\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. \u003c\/p\u003e\n\u003ch2\u003ePublication\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7791757\/#\"\u003e\u003cstrong\u003eJ Exp Clin Cancer Res. 2021; 40: 22.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9894309\/#\"\u003e\u003cstrong\u003eCell Genom. 2022 Nov 9; 2(11): 100191.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Forzen Vial","offer_id":45329745248437,"sku":"H-6040","price":783.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":45329745281205,"sku":"H-6040","price":814.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":45329745313973,"sku":"H-6040","price":886.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EpithelialCells_copy-500x500_4f7435f3-ed07-4b9b-bafa-4a774603f6af.jpg?v=1778250359"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00001_f4acf635-a048-4a82-8583-310f509e1f18.jpg?v=1775721646","url":"https:\/\/biofargo.com\/collections\/primary-cells.oembed","provider":"Biofargo","version":"1.0","type":"link"}