{"title":"PCR Master Mixes \u0026 Reagents","description":"","products":[{"product_id":"1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-146","title":"1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes complementary DNA strands on RNA templates from gene-specific primers,\u003cem\u003e ≥1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704700733\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X1mL \/ No ROX","offer_id":43079388856501,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ Low ROX","offer_id":43079388922037,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ High ROX","offer_id":43079388954805,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ No ROX","offer_id":43079388987573,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ Low ROX","offer_id":43079389020341,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ High ROX","offer_id":43079389053109,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079389085877,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079389118645,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079389151413,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-10ml.jpg?v=1732863909"},{"product_id":"1-step-2x-super-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-149","title":"1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eReverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, with ≥1 unit per 20μL of reaction.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase consistently reversely transcribes single-digit copies of target RNA molecules.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains Super\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704701307\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414918299829,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414918332597,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414918365365,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414918398133,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414918430901,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414918463669,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414918496437,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414918529205,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414918561973,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-2x1mL.jpg?v=1732863930"},{"product_id":"1-step-2x-rt-pcr-master-mix-sybr-green-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X \u003cem\u003eFast\u003c\/em\u003e RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA polymerase for PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-UM_Master_Mix_SYBR_Green_10232023.pdf?v=1704700356\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414936191157,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414936223925,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414936256693,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414936289461,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414936322229,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414936354997,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414936387765,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414936420533,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414936453301,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-2x1mL.jpg?v=1732863916"},{"product_id":"2x-qpcr-master-mix-taqman-probe-cat-qpcr-153","title":"2X qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThis kit uses \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-UM_qPCR_TaqMan_02062023.pdf?v=1704701539\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414982525109,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414982557877,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414982590645,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414982623413,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414982656181,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414982688949,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414982721717,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414982754485,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414982787253,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-10ml.jpg?v=1732864822"},{"product_id":"2x-fast-qpcr-master-mix-sybr-green-cat-qpcr-157","title":"2X Fast qPCR Master Mix SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Fast qPCR Master Mix is used for real-time qualitative and quantitative qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit contains \u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which can extend more than 300 bases with a short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermo-cycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-UM_qPCR_Fast_SYBR_Green_02062023.pdf?v=1704701908\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415301062837,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415301095605,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415301128373,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415301161141,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415301193909,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415301226677,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415301259445,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415301292213,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415301324981,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-10ml.jpg?v=1732864809"},{"product_id":"2x-super-multiplex-qpcr-master-mix-taqman-probe-cat-qpcr-159","title":"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Multiplex Super qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to four targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eProduct Characterizations\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for multiplex qPCR with TaqMan probes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit uses Super \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and  produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eTwo to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250716159-INSTRUCTION-03012023_1_1.pdf?v=1752655287\" title=\"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415318692021,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415318724789,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415318757557,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415318790325,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415318823093,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415318855861,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415318888629,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415318921397,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415318954165,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/159-10ml.jpg?v=1732864829"},{"product_id":"mag-beads-his-tag-protein-purification","title":"Mag-Beads His-Tag Protein Purification","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"desc-box\"\u003e\n      \u003cp style=\"margin:0;\"\u003eMag-Beads His Tag protein purification magnetic beads are a new type of purification material developed for purifying His-tag proteins. They can directly purify high-purity target proteins from biological samples through a unique magnetic separation method, greatly simplifying the purification process and improving purification efficiency. They are suitable for convenient purification of His-tag proteins in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specification --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table w-640\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n            \u003cth\u003eSpecification\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003e10 ML£¨10%V\/V)\/25 ML£¨10%V\/V)\/100 ML£¨10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eMag-Beads His-Tag Protein Purification\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComposition\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eSubstrate\u003c\/td\u003e\n            \u003ctd\u003eMagnetic agarose microspheres\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eAverage Particle Size\u003c\/td\u003e\n            \u003ctd\u003e0\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eParticle Size\u003c\/td\u003e\n            \u003ctd\u003e30 ¦Ìm~150 ¦Ìm\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eProtein Binding Ability\u003c\/td\u003e\n            \u003ctd\u003e\u0026gt;10 mg histidine labeled protein\/ml (pure magnetic beads)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n            \u003ctd\u003e20% ethanol\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eMagnetic Bead Ratio\u003c\/td\u003e\n            \u003ctd\u003eMagnetic beads suspended in protective solution with a content of 10% (V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComponent\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eFeatures\u003c\/h2\u003e\n    \u003cul\u003e\n      \u003cli\u003eDirectly purifying crude protein reduces equipment, manpower, and time costs, and is more suitable for large-scale protein purification production.\u003c\/li\u003e\n      \u003cli\u003eOne step purification can obtain up to 95% purity of the target protein.\u003c\/li\u003e\n      \u003cli\u003eGood stability, not affected by flow velocity.\u003c\/li\u003e\n      \u003cli\u003eReusable.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eSuitable for convenient purification of His-tag protein in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003e2~8¡ãC, do not freeze\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ml(10%V\/V)","offer_id":52400870097077,"sku":"P310137-10ml(10%V\/V)","price":148.0,"currency_code":"USD","in_stock":true},{"title":"25ml(10%V\/V)","offer_id":52400870129845,"sku":"P310137-25ml(10%V\/V)","price":302.0,"currency_code":"USD","in_stock":true},{"title":"100ml(10%V\/V)","offer_id":52400870162613,"sku":"P310137-100ml(10%V\/V)","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_magbeads.png?v=1751472361"},{"product_id":"extraction-free-1-step-2x-rt-pcr-master-mix-sybr-green-dye","title":"Extraction-Free 1-Step 2X RT-PCR Master Mix, SYBR Green Dye (#142)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142) enables real-time qualitative and quantitative RT-PCR directly from cultured cells without the need for RNA purification. This 2X premixed solution contains all necessary components for one-step RT-PCR except gene-specific primers and RNA templates.\u003c\/p\u003e\n\u003cp\u003eIt streamlines gene expression analysis by allowing direct amplification from crude lysates, eliminating time-consuming and laborious RNA purification steps.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step RT-qPCR workflow: reverse transcription and PCR amplification combined in a single tube.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRobust performance: supports 10 to 100,000 cells per sample, results comparable to purified RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEconomic and efficient: \u003cbr\u003e2 × 1 mL packaging—200 reactions at 20 μL or 400 reactions at 10 μL final volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSYBR Green detection method.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatible with most real-time PCR instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTry Before You Buy — \u003cstrong\u003eRequest a trial sample.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eWhy Choose Biofargo #142?\u003c\/h3\u003e\n\u003cp\u003eOur Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142), is designed as a cost-effective alternative to Thermo Fisher's Cells-to-CT™ 1-Step Power SYBR™ Green Kit.\u003c\/p\u003e\n\u003cp\u003eWith comparable performance in gene expression analysis directly from cell lysates, our kit offers reaction costs as low as $4 per reaction—ideal for both research laboratories and high-throughput applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003eThe 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44624578314421,"sku":"RT-PCR-142-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44624578347189,"sku":"RT-PCR-142-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44624578379957,"sku":"RT-PCR-142-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44624578412725,"sku":"RT-PCR-142-LowRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44624578445493,"sku":"RT-PCR-142-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44624578478261,"sku":"RT-PCR-142-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44624578511029,"sku":"RT-PCR-142-HighRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44624578543797,"sku":"RT-PCR-142-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44624578576565,"sku":"RT-PCR-142-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133256.jpg?v=1732871896"},{"product_id":"extraction-free-rt-pcr-master-mix-taqman-probe-149","title":"1-Step 2X Super multiplex RT-PCR Master Mix-TaqMan Probe (#149)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eThis one-step 2× multiplex RT-PCR Master Mix is designed for real-time qualitative and quantitative amplification with TaqMan probes directly from extraction-free RNA samples. The 2× premixed solution provides all necessary components except gene-specific primers, TaqMan probes, and RNA templates. RT-PCR 149 is optimized for high-GC templates and stem-loop rich regions, and supports multiplex detection of up to two targets, ensuring reliable reverse transcription and amplification of challenging RNA templates.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eKit Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix contains extra more \u003c\/span\u003eredundant amounts of the RTase and DNA polymerase,\u003cspan\u003e specifically designed to overcome endogenous inhibition in extraction-free RNA samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eHarvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHeat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRun RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091748\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44628592099509,"sku":"RT-PCR-149-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44628592132277,"sku":"RT-PCR-149-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44628592165045,"sku":"RT-PCR-149-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44628592197813,"sku":"RT-PCR-149-LowRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44628592230581,"sku":"RT-PCR-149-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44628592263349,"sku":"RT-PCR-149-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44628592296117,"sku":"RT-PCR-149-HighRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44628592328885,"sku":"RT-PCR-149-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44628592361653,"sku":"RT-PCR-149-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a9625ef0e407146a060016379075c19d.jpg?v=1757990155"},{"product_id":"cat-rt-pcr-146-1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe","title":"Extraction-Free 1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe (#146)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers at ≥1 unit per 20 μL of reaction.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe preferable PCR product size is ≤150bp.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e1.Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2.Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e3.Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e4.Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5.Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091746\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2X 1mL","offer_id":44869815828661,"sku":"RT-PCR-146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5X 1mL","offer_id":44869815861429,"sku":"RT-PCR-146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 10mL","offer_id":44869815894197,"sku":"RT-PCR-146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2X 1mL","offer_id":44869815926965,"sku":"RT-PCR-146-LowRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5X 1mL","offer_id":44869815959733,"sku":"RT-PCR-146-LowRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 10mL","offer_id":44869815992501,"sku":"RT-PCR-146-LowRox-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2X 1mL","offer_id":44869816025269,"sku":"RT-PCR-146-HighRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5X 1mL","offer_id":44869816058037,"sku":"RT-PCR-146-HighRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 10mL","offer_id":44869816090805,"sku":"RT-PCR-146-HighRox-1","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133240_1_1.jpg?v=1733237326"},{"product_id":"animal-tissue-direct-pcr-kit","title":"Animal Tissue Direct PCR Kit","description":"\u003ch2\u003eDescprition\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThis product uses a unique lysis buffer system to rapidly release genomic DNA from animal tissue samples for PCR reactions, so it is especially suitable for large-scale genetic testing.\u003c\/p\u003e\n\u003cp\u003eThe process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C, and the released trace amount of DNA can be used as a template for PCR reaction without other processes such as protein removal and RNA removal.\u003c\/p\u003e\n\u003cp\u003e2×PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer, and stabilizer. It can be used in combination with lysis buffer to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb\/min, which is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo need for time-consuming and expensive DNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe sample requirement is small, as little as 5 mg of animal tissue can be used for experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNo special treatment such as grinding and crushing is required, and the operation is simple.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe optimized PCR system makes PCR more specific and more resistant to PCR reaction inhibitors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eScope of application: a variety of animal tissues.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA released from sample lysis: Use as PCR template only.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit can be used for the following purposes: identification of transgenes, animal genotyping, etc.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220711110927_12625_600x600.png?v=1744186659\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2 style=\"text-align: start;\"\u003eStorage\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThis kit Part I is stored at room temperature or 2-8℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReagent Buffer AL can be stored for 12 months under dry conditions; for longer storage, it can be stored at 2–8°C.\u003c\/p\u003e\n\u003cp\u003eNote: If stored at low temperature, the solution is prone to precipitation. Be sure to place the solution in the kit at room temperature for a period of time before use, and if necessary, preheat in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix well before use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReagent Foregene Protease has a unique formula, in order to ensure its better activity and stability, please store it at 4℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReagent 6×DNA Loading Buffer, can be stored at 4°C or -20°C for a long time.\u003c\/p\u003e\n\u003cp\u003eThis kit Part II is stored at -20°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReagent 2×PCR EasyTM Mix, if used frequently, it can also be stored at 4°C for a short time (limited to be used up within 10 days).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"text-align: start;\"\u003eDocuments\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Animal_Tissue_Direct_PCR_Kit_Instruction_Manual-V1.0-EN_8b37ddd9-3984-4d17-869e-a0821bf8a2e3.docx?v=1752827813\"\u003e\u003cstrong\u003eAnimal Tissue Direct PCR Kit Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45280082067637,"sku":"TP-01111","price":417.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45280082100405,"sku":"TP-01113","price":2601.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/AnimalTissueDirectPCRKit.jpg?v=1744186488"},{"product_id":"animal-tissue-direct-pcr-kit65-lysis-ung","title":"Animal Tissue Direct PCR Kit(65℃ lysis)-UNG","description":"\u003ch2\u003eDescprition\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThis kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.\u003c\/p\u003e\n\u003cp\u003eThe process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as template for PCR reaction.\u003c\/p\u003e\n\u003cp\u003e2×PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2×PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of PCR products contamination during large-scale genetic testing.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb\/min, which is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo time-consuming and expensive DNA purification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe sample demand is small, as little as 5mg animal tissue can be tested.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNo special treatment such as grinding and crushing is required, and the operation is simple.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOptimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAnti-pollution PCR system 2×PCR EasyTM Mix (UNG) effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eApplication: a variety of animal tissues.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA released from sample lysis: only used as a PCR template.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220711113528_22668_600x600.png?v=1744187355\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003ePart I of this kit should be stored at 2-8℃.\u003c\/p\u003e\n\u003cp\u003eThe reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.\u003c\/p\u003e\n\u003cp\u003e Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.\u003c\/p\u003e\n\u003cp\u003e Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.\u003c\/p\u003e\n\u003cp\u003ePart II of this kit should be stored at -20℃ .\u003c\/p\u003e\n\u003cp\u003e Reagent 2×PCR EasyTM Mix(UNG), if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Animal_Tissue_Direct_PCR_Kit-UNG_Instruction_Manual-V1.0-EN_6154dfce-99ed-4565-8824-4e02f0c4cca5.docx?v=1752827904\"\u003e\u003cstrong\u003eAnimal Tissue Direct PCR Kit-UNG Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45280104284341,"sku":"TP-01121","price":478.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45280104317109,"sku":"TP-01123","price":3225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-01121AnimalTissueDirectPCRKit_65_lysis_-UNG_5.jpg?v=1744187527"},{"product_id":"mouse-tail-superdirect™-pcr-kitroom-temperature-lysis","title":"Mouse Tail SuperDirect™  PCR Kit(room temperature lysis)","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eThis kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.\u003c\/p\u003e\n\u003cp\u003eThe process of releasing genomic DNA from the lysis buffer is completed \u003cstrong\u003ewithin 10-30 minutes\u003c\/strong\u003e at \u003cstrong\u003eroom temperature (20-25°C).\u003c\/strong\u003e No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.\u003c\/p\u003e\n\u003cp\u003e2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb\/min, which is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo time-consuming and expensive DNA purification is required.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEasy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eForegene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOptimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003ePart I of this kit should be stored at 2-8℃.\u003c\/p\u003e\n\u003cp\u003eThe reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.\u003c\/p\u003e\n\u003cp\u003eReagent Foregene Protease Plus I has a unique formula. To ensure its better activity and stability, please store it at 4℃.\u003c\/p\u003e\n\u003cp\u003eReagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.\u003c\/p\u003e\n\u003cp\u003ePart II of this kit should be stored at -20℃ .\u003c\/p\u003e\n\u003cp\u003eReagent 2×M-PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45280112378037,"sku":"TP-01311","price":417.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45280112410805,"sku":"TP-01313","price":2601.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-01311MouseTailSuperDirectTMPCRKit_roomtemperaturelysis_4.jpg?v=1744188221"},{"product_id":"blood-superdirect™-pcr-kit-heparin","title":"Blood SuperDirect™ PCR Kit (Heparin)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis product uses a unique PCR buffer system, which allows direct PCR with whole blood samples as a template without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.\u003c\/p\u003e\n\u003cp\u003e2× SuperEasyTM Mix-Heparin contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhncer, optimizer and stabilizer.\u003c\/p\u003e\n\u003cp\u003eTaq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb\/min, which is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003cp\u003eAccording to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.\u003c\/p\u003e\n\u003ch2\u003eFeature\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2× SuperEasyTM Mix-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe sample is fully enclosed operation, so there is no need to worry about sample contamination and false positive PCR results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220713100456_86072_600x600.png?v=1744257098\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThis kit should be stored at -20℃.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e2× SuperEasyTM Mix-Heparin,If used frequently, it can be stored at 4°C for short-term storage (use up within 10 days).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45282765111477,"sku":"TP-04211","price":417.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45282765144245,"sku":"TP-04213","price":2601.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-04211BloodSuperDirectTMPCRKit_Heparin_4.jpg?v=1744257412"},{"product_id":"plant-leaf-direct-pcr-kit-ung","title":"Plant Leaf Direct PCR Kit-UNG","description":"\u003ch2\u003eDescription\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eBased on Plant Leaf Direct PCR kit, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added. In this way, the specificity and accuracy of amplification are ensured, and the problem of PCR product contamination that may occur during large-scale genetic testing is prevented.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo time - consuming and expensive DNA purification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLess material\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSimple— the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2X Mix— reducing sample loading errors and reaction system preparation time\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast— template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh throughput—\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003elysis reaction can be completed in 96-well PCR plate\u003c\/p\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220119134803_71454_600x600.jpg?v=1744267159\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch3 style=\"text-align: start;\"\u003eElectropherogram\u003c\/h3\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220119134820_17798_600x600.jpg?v=1744267242\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003ch2 style=\"text-align: start;\"\u003eStorage\u003c\/h2\u003e\n\u003cp\u003ePart I of this kit should be stored at room temperature or 2-8℃.\u003c\/p\u003e\n\u003cp\u003evReagents Buffer P1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.\u003c\/p\u003e\n\u003cp\u003ePart II of this kit should be stored at -20℃.\u003c\/p\u003e\n\u003cp\u003evReagents Buffer 2×Leaf PCR EasyTM Mix(UNG), if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45283238805685,"sku":"TP-02121","price":478.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45283238838453,"sku":"TP-02123","price":3225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-02121PlantLeafDirectPCRKit-UNG_3.jpg?v=1744268604"},{"product_id":"plant-leaf-direct-pcr-plus-kit-polysaccharide-polyphenol-plant","title":"Plant Leaf Direct PCR Plus Kit (Polysaccharide polyphenol plant)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePlant Leaf Direct PCR Plus Kit adopts a unique lysis buffer system, which can rapidly release genomic DNA from leaf samples of plants with high polysaccharide and polyphenol content (such as cotton, banana, etc.) for PCR reaction. The leaves do not need to be ground or shredded when treated with lysis buffer, making them ideal for large-scale genetic testing.\u003c\/p\u003e\n\u003cp\u003eThe process of releasing genomic DNA from the lysis buffer can be completed within 5-10 minutes, without the need for other processes of deproteinization, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction.\u003c\/p\u003e\n\u003cp\u003e2× Leaf PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can directly use the cleavage product of plant material as a template for efficient and specific amplification. The reagent contains D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially engineered for direct PCR reactions. It can be used in combination with direct lysate to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase is a DNA polymerase specially developed for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb\/min. It is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003cp\u003eAccording to the differences of plant samples, the products are divided into two categories: Plant Leaf Direct PCR Kit and Plant Leaf Direct PCR Plus Kit for polysaccharides and polyphenols; in the PCR detection stage, it can be According to the experimental needs, choose the common 2× Leaf PCR EasyTM Mix or the 2× Leaf PCR EasyTM Mix (UNG) which has the effect of preventing the contamination of PCR amplification products.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo time-consuming and expensive DNA purification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLess material\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSimple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2X Mix—reducing sample loading errors and reaction system preparation time\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh throughput—lysis reaction can be completed in 96-well PCR plate\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220119113415_47323_600x600.jpg?v=1744269006\"\u003e\u003c\/div\u003e\n\u003ch3 style=\"text-align: start;\"\u003eElectropherogram\u003c\/h3\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220119113440_25007_600x600.jpg?v=1744269042\"\u003e\u003c\/div\u003e\n\u003ch2 style=\"text-align: start;\"\u003eStorage \u003c\/h2\u003e\n\u003cp\u003ePart I of this kit should be stored at room temperature or 2-8℃.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReagents Buffer P1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.\u003c\/p\u003e\n\u003cp\u003ePart II of this kit should be stored at -20℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReagents Buffer 2×Leaf PCR EasyTM Mix, if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Plant_Leaf_Direct_PCR_Plus_Kit_Instruction_Manual-V1.0-EN_8798f73e-78f5-4295-9839-690919f5fd4d.docx?v=1752831733\"\u003e\u003cstrong\u003ePlant Leaf Direct PCR Plus Kit Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45283251749045,"sku":"TP-02131","price":448.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45283251781813,"sku":"TP-02133","price":2913.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-02131PlantLeafDirectPCRPlusKit_2.jpg?v=1744269141"},{"product_id":"plant-seed-direct-pcr-plus-kit-i-ung","title":"Plant Seed Direct PCR Plus Kit I-UNG","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eDirectly use polysaccharide polyphenol seed lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45283622289589,"sku":"TP-03161","price":754.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45283622322357,"sku":"TP-03163","price":6450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-03161PlantSeedDirectPCRPlusKitI-UNG_7.jpg?v=1744275860"},{"product_id":"plant-seed-direct-pcr-plus-kit-ii-ung","title":"Plant Seed Direct PCR Plus Kit II-UNG","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eThis product uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with high polysaccharides and polyphenols (such as bananas, cotton, etc.) for use in PCR reactions, so it is particularly suitable for large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for the seed samples that still need to be germinated, you can choose to cut out micro-tissue pieces (1-5mg) other than the seed embryos for experiments; for experiments that require sampling and testing in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).\u003c\/p\u003e\n\u003cp\u003eThe process of releasing genomic DNA from the cleavage reaction system can be completed within 5-10 minutes. There is no need for other processes to remove protein, RNA or secondary metabolites, and the released trace amounts of DNA can be used as a template for PCR reactions.\u003c\/p\u003e\n\u003cp\u003e2× Seed PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can directly use the lysis mixture of plant materials as a template for efficient and specific amplification. This reagent contains the company's D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially modified for direct PCR. Used in conjunction with the lysis reaction system can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.\u003c\/p\u003e\n\u003cp\u003e2× Seed PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× Seed PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.\u003c\/p\u003e\n\u003cp\u003eD-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb\/min, which is especially suitable for Direct PCR reaction.\u003c\/p\u003e\n\u003cp\u003eAccording to the differences in plant samples, the products are divided into two categories: Plant Seed Direct PCR Kit and Plant Seed Direct PCR Plus Kit. In the lysis stage, considering that different types of seeds or tissue materials vary greatly in the amount of reagents during the lysis process, customers can choose the corresponding plant seed (small and medium) direct PCR kit or plant seed (large) according to the size of their experimental materials Direct PCR kit; in the PCR detection stage, customers can also choose ordinary 2× Seed PCR EasyTM Mix or 2× Seed PCR EasyTM Mix (UNG) which can prevent the contamination of PCR amplification products according to the experimental needs.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eNo time-consuming and expensive DNA purification is required.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe sample demand is small, just take a single seed.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNo special treatments such as grinding and crushing are required, and the operation is simple.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20220119134237_83385_c3c29554-ee2c-431b-bd15-0b34e72196ca_600x600.png?v=1744276945\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTransportation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit Part II is in a state of \u0026lt;4 ℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePart I of this kit should be stored at room temperature or 2-8°C.\u003c\/p\u003e\n\u003cp\u003ePart II should be stored at -20°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Foregene","offers":[{"title":"200 ×20μl rxns","offer_id":45283643031733,"sku":"TP-03181","price":895.0,"currency_code":"USD","in_stock":true},{"title":"2000 ×20μl rxns","offer_id":45283643064501,"sku":"TP-03183","price":7387.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/TP-03181PlantSeedDirectPCRPlusKitII-UNG_4.jpg?v=1744276767"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00001_8ccb1149-1cc5-4174-90c2-8431098c641e.jpg?v=1775716971","url":"https:\/\/biofargo.com\/collections\/pcr-master-mixes-reagents.oembed","provider":"Biofargo","version":"1.0","type":"link"}