{"title":"PCR Master Mix","description":"","products":[{"product_id":"copy-of-thermophilic-reverse-transcriptase-cat-rt-pcr-141","title":"Taq-Probe Polymerase-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThis DNA-dependent DNA polymerase is specially engineered for real-time PCR and RT-PCR amplification with \u003c\/span\u003eTaqMan probe, singleplex or multiplex.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eIt is used together with Thermophilic Reverse Transcriptate (Cat 140) for RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe polymerase is specially engineered for TaqMan probe, its increased 5’-3’ exonuclease activity generating S-shaped curve.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 5x buffer has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e  \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/145-UM_Taq-Probe_polymerase_10022023.pdf?v=1704699039\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"250 rxns","offer_id":42920662991029,"sku":"145-250","price":124.0,"currency_code":"USD","in_stock":true},{"title":"1250 rxns","offer_id":42920663285941,"sku":"145-1250","price":581.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/145-500U-C.jpg?v=1732884330"},{"product_id":"copy-of-taq-probe-polymerase-cat-rt-pcr-145","title":"Taq-Fast Polymerase-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e \u003cstrong\u003eIntended use\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThis DNA-dependent DNA polymerase is used real-time PCR and RT-PCR amplification with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eIt is used together with Thermophilic Reverse Transcriptase (Cat 140) for RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast polymerase extends more than 300 bases with short PCR cycling program. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 5x buffer has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e  \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-UM_Taq-Fast_polymerase_07282023.pdf?v=1704699580\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"500U","offer_id":42920672854197,"sku":"148-500","price":103.0,"currency_code":"USD","in_stock":true},{"title":"2500U","offer_id":42920672952501,"sku":"148-2500","price":474.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/148-500U-B.jpg?v=1732884325"},{"product_id":"1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-146","title":"1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes complementary DNA strands on RNA templates from gene-specific primers,\u003cem\u003e ≥1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704700733\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X1mL \/ No ROX","offer_id":43079388856501,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ Low ROX","offer_id":43079388922037,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"2X1mL \/ High ROX","offer_id":43079388954805,"sku":"146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ No ROX","offer_id":43079388987573,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ Low ROX","offer_id":43079389020341,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"5X1mL \/ High ROX","offer_id":43079389053109,"sku":"146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079389085877,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079389118645,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079389151413,"sku":"146-10","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-10ml.jpg?v=1732863909"},{"product_id":"1-step-2x-fast-rt-pcr-master-mix-sybr-green-cat-rt-pcr-147","title":"1-Step 2X Fast RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step Fast 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-UM_Fast_Master_Mix_SYBR_Green_07282023.pdf?v=1704701048\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079401439413,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079401472181,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079401504949,"sku":"147-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079401537717,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079401570485,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079401603253,"sku":"147-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079401636021,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079401668789,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079401701557,"sku":"147-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/147-2x1mL.jpg?v=1732863901"},{"product_id":"1-step-2x-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e \u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe 1-Step 2X RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and RNA template.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" data-rte-list=\"default\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit is designed for singleplex RT-PCR with TaqMan probe.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase efficiently synthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤1 unit per 20μL of reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\" class=\"\"\u003eThe kit also contains Taq-Probe DNA polymerase specially engineered for TaqMan probe, generating S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe concentrations of the primers and probe are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe preferable PCR product size is ≤150bp.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003e\n\u003cp\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-UM_Master_Mix_TaqMan_07282023.pdf?v=1704700000\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2X 1mL \/ No ROX","offer_id":43079478214837,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ Low ROX","offer_id":43079478280373,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"2X 1mL \/ High ROX","offer_id":43079478313141,"sku":"143-2","price":309.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ No ROX","offer_id":43079478345909,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ Low ROX","offer_id":43079478378677,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"5X 1mL \/ High ROX","offer_id":43079478411445,"sku":"143-5","price":755.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ No ROX","offer_id":43079478444213,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ Low ROX","offer_id":43079478476981,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true},{"title":"10mL \/ High ROX","offer_id":43079478509749,"sku":"143-10","price":1453.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/143-10ml.jpg?v=1732863923"},{"product_id":"1-step-2x-super-multiplex-rt-pcr-master-mix-taqman-probe-cat-rt-pcr-149","title":"1-Step 2X Super Multiplex RT-PCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X Super Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Characteristics\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eReverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers, with ≥1 unit per 20μL of reaction.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase consistently reversely transcribes single-digit copies of target RNA molecules.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains Super\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023.pdf?v=1704701307\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414918299829,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414918332597,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414918365365,"sku":"149-2","price":463.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414918398133,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414918430901,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414918463669,"sku":"149-5","price":1130.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414918496437,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414918529205,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414918561973,"sku":"149-10","price":2173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-2x1mL.jpg?v=1732863930"},{"product_id":"1-step-2x-rt-pcr-master-mix-sybr-green-cat-rt-pcr-144","title":"1-Step 2X RT-PCR Master Mix-SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 1-Step 2X \u003cem\u003eFast\u003c\/em\u003e RT-PCR Master Mix is used for real-time qualitative and quantitative RT-PCR amplifications with SYBR Green dye particularly for fast thermocycling.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and RNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003eCharacteristics\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit is designed for singleplex RT-PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA polymerase for PCR with SYBR Green dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-UM_Master_Mix_SYBR_Green_10232023.pdf?v=1704700356\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414936191157,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414936223925,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414936256693,"sku":"144-2","price":264.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414936289461,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414936322229,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414936354997,"sku":"144-5","price":645.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414936387765,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414936420533,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414936453301,"sku":"144-10","price":1240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/144-2x1mL.jpg?v=1732863916"},{"product_id":"2x-qpcr-master-mix-taqman-probe-cat-qpcr-153","title":"2X qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X qPCR Master Mix is used for real-time qualitative and quantitative qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probe and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with TaqMan probe.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThis kit uses \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probe, which increased 5’ to 3’ exonuclease efficiency produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probe are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-UM_qPCR_TaqMan_02062023.pdf?v=1704701539\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43414982525109,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43414982557877,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43414982590645,"sku":"153-2","price":174.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43414982623413,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43414982656181,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43414982688949,"sku":"153-5","price":423.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43414982721717,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43414982754485,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43414982787253,"sku":"153-10","price":796.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/153-10ml.jpg?v=1732864822"},{"product_id":"2x-multiplex-qpcr-master-mix-taqman-probe-cat-qpcr-156","title":"2X Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to 4 targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for multiplex qPCR with TaqMan probes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit uses \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eTwo to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/156-UM_qPCR_Multiplex_TaqMan_02062023.pdf?v=1704701718\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415292149941,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415292182709,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415292215477,"sku":"156-2","price":208.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415292248245,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415292281013,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415292313781,"sku":"156-5","price":506.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415292346549,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415292379317,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415292412085,"sku":"156-10","price":953.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/156-10ml.jpg?v=1732864815"},{"product_id":"2x-fast-qpcr-master-mix-sybr-green-cat-qpcr-157","title":"2X Fast qPCR Master Mix SYBR Green-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eIntended Use\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Fast qPCR Master Mix is used for real-time qualitative and quantitative qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers and DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProduct\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eCharacterizations\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for singleplex qPCR with SYBR Green dye.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit contains \u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which can extend more than 300 bases with a short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermo-cycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-UM_qPCR_Fast_SYBR_Green_02062023.pdf?v=1704701908\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415301062837,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415301095605,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415301128373,"sku":"157-2","price":132.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415301161141,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415301193909,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415301226677,"sku":"157-5","price":323.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415301259445,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415301292213,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415301324981,"sku":"157-10","price":607.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/157-10ml.jpg?v=1732864809"},{"product_id":"2x-super-multiplex-qpcr-master-mix-taqman-probe-cat-qpcr-159","title":"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools","description":"\u003ch2 data-mce-fragment=\"1\"\u003eIntended Use\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe 2X Multiplex Super qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to four targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and  templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eProduct Characterizations\u003c\/h2\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit is designed for multiplex qPCR with TaqMan probes.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit uses Super \u003cem\u003eTaq\u003c\/em\u003e-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and  produces S-shaped curve.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eTwo to four pairs of gene-specific primers can be applied in one reaction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (User Manual Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentrations for your choice (see User Manual Table 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250716159-INSTRUCTION-03012023_1_1.pdf?v=1752655287\" title=\"2X Super Multiplex qPCR Master Mix-TaqMan Probe-Genetic Tools\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"2×1ml \/ No ROX","offer_id":43415318692021,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ Low ROX","offer_id":43415318724789,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"2×1ml \/ High ROX","offer_id":43415318757557,"sku":"159-2","price":228.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ No ROX","offer_id":43415318790325,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ Low ROX","offer_id":43415318823093,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml \/ High ROX","offer_id":43415318855861,"sku":"159-5","price":557.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ No ROX","offer_id":43415318888629,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ Low ROX","offer_id":43415318921397,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true},{"title":"10ml \/ High ROX","offer_id":43415318954165,"sku":"159-10","price":1048.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/159-10ml.jpg?v=1732864829"},{"product_id":"2x-taq-pcr-mix-kleanab","title":"2X Taq PCR Mix-KleanAB","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Table SOP (Embedded)\n     - Wrapper scroll + table min-width\n     - Strong selector weight via .biofargo-wrap prefix\n     - 2-column table default: 640px\n  ========================== *\/\n  .biofargo-wrap .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n    margin-top:15px;\n  }\n  .biofargo-wrap table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:640px; \/* 2-column table *\/\n    background:#fff;\n  }\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td{\n    border:1px solid #e9ecef;\n    padding:15px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  \/* If you ever add a header row later *\/\n  .biofargo-wrap table.biofargo-table thead th{\n    background:#f2f2f2;\n    color:#000;\n    font-weight:700;\n    text-align:left;\n  }\n  \/* Parameter\/Item column *\/\n  .biofargo-wrap table.biofargo-table tbody td:first-child{\n    background:#f8f9fa;\n    font-weight:600;\n    width:30%;\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Description\n    \u003c\/h2\u003e\n    \u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n      This reagent kit is fast and convenient to use, and can be used for the rapid establishment of ordinary PCR systems and other PCR related experiments. 2X SanTaq PCR Mix includes MgCl2, dNTP, Taq DNA Polymerase, PCR buffer, loading, and PCR enhancer. The optimized PCR Mix enables most PCR reactions to proceed well. The preparation of the PCR reaction system can be completed within a few minutes by adding primers, template DNA, and an appropriate amount of deionized water.\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Specification\n    \u003c\/h2\u003e\n\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003e2X Taq PCR Mix\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eConcentration\u003c\/td\u003e\n            \u003ctd\u003e25 μg \/ 500 μl\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eColor\u003c\/td\u003e\n            \u003ctd\u003eBlue\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Features\n    \u003c\/h2\u003e\n    \u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThe product contains bromophenol blue dye, and the PCR product can be directly subjected to electrophoresis.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 12px; padding-left: 5px;\"\u003eThis product contains excellent stabilizers and can be stored for 3 months at 4 ℃.\u003c\/li\u003e\n      \u003cli style=\"margin-bottom: 0; padding-left: 5px;\"\u003eThe PCR product has a 3 '- dA protruding end and can be easily cloned into the T vector.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n    \u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n      \u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e\n      Applications\n    \u003c\/h2\u003e\n\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n        \u003cli style=\"margin-bottom: 0;\"\u003eConventional PCR\u003c\/li\u003e\n      \u003c\/ul\u003e\n      \u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"padding: 30px;\"\u003e\n    \u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 40px;\"\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eComponents\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background-color: #fff; font-size: 14px;\"\u003e\n          2X SanTaq PCR Mix (with blue dye)\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n        \u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eStorage\u003c\/h2\u003e\n        \u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background-color: #fff5f5; color: #c53030; font-weight: bold; font-size: 18px; border-radius: 4px;\"\u003e\n          -20°C\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Sangon","offers":[{"title":"1ml","offer_id":43839167398069,"sku":"P310149-0100","price":8.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P310149_1.jpg?v=1732864843"},{"product_id":"2-phanta-flash-master-mix-dye-plus","title":"2 x Phanta Flash Master Mix (Dye Plus)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e2 × Phanta Flash Master Mix is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec\/kb) while maintaining high fidelity and yield. The amplification error rate of Phanta Flash Super-Fidelity DNA Polymerase is 81-fold lower than that of conventional \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer\/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This product contains tracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eHigh fidelity:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e ~81X higher than \u003cem\u003eTaq\u003c\/em\u003e DNA Polymerase\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFlash amplification: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eExtension speed up to 5 sec\/kb\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eRobust performance: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuperior specificity, sensitivity, and yields\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eBroad Compatibility: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eSuitable for complex samples \u0026amp; GC-rich systems\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eP520-01\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-02\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e P520-03\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e2 × Phanta Flash Master Mix (Dye Plus)\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e5×1ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e15×1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExperimental Cases\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2 × Phanta Flash Master Mix (Vazyme#P510), 2 × Phanta Flash Master Mix (Dye Plus) (Vazyme#P520) and other commercially available brands of high-fidelity polymerases were used to perform PCR amplification. The target fragment of 353 bp and 873 bp were amplified at an extension time of 1 sec\/kb, 7140 bp fragment was amplified at an extension time of 4 sec\/kb and 5 sec\/kb, respectively. The results show that all the fragments could be successfully amplified by P510 and P520.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1.png?v=1765791826\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUltra-fast Amplification\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAmplification of fragments ≤10 kb can be achieved within 1 h!\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_2cd7730b-c2f8-4911-b129-4685a4c7e4f7.png?v=1765791869\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Specificity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eVazyme#P510, Vazyme#P520 and other commercially available brands of high-fidelity polymerases were used to amplify different length of fragments in accordance to the recommended systems and procedures of respective instructions. The results show that P510 and P520 have higher amplification specificity than other brands of high-fidelity polymerases.\u003cbr\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/911f6c1e575e4eb9b036c704362f9aa8_1_1d7164bb-8d83-4cfa-8c65-f27b25d4f9f7.png?v=1765791908\" alt=\"\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at -30 ~ -15°C and transport at ≤0°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ1: Does the tracking dye in 2 × Phanta Flash Master Mix (Dye Plus) interfere with downstream applications?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA1: The tracking dye (which consists of Bromophenol Blue) is compatible with downstream applications such as fluorescent automated DNA sequencing, ligation, and restriction digestion. For applications that require analysis of PCR products by absorbance or fluorescence excitation, we recommend using colorless versions of the products Phanta Flash Super-Fidelity DNA Polymerase or 2 × Phanta Flash Master Mix or purifying the PCR products prior to analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ2: Can I perform restriction digestion in the Phanta Flash reaction buffer?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA2: Phanta Flash reaction buffer is compatible with restriction digestion directly after PCR. Note that Phanta Flash Super-Fidelity DNA Polymerase is not inactivated during PCR, thus purification of PCR products before restriction digestion is recommended if intact 5'- or 3'- overhangs are needed for cloning.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ3: Can I amplify GC-rich targets with Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA3: Phanta Flash Super-Fidelity DNA Polymerase can amplify targets with high GC content (up to 83% GC) without any additional DNA melting agents. In cases of extremely GC-rich targets (\u0026gt;83% GC), addition of DMSO to final concentration of 5% is recommended.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ4: What are your recommendations when working with long amplicons using Phanta Flash Super-Fidelity DNA Polymerase?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA4: Good lab practices are important for long fragment amplification. These include using high-quality templates (pure, fresh, and intact) and fresh primer solutions. Reducing primer concentration to 0.3 µM may also improve the results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQ5: What are your recommendations when calculating annealing temperatures using Phanta Flash Super-Fidelity DNA Polymerase?\u003cbr\u003e\u003c\/strong\u003eA5: Approximately 5°C above Tm of primers, but no more than 68°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Manual_P520_V23.1.pdf?v=1716346848\" type=\"application\/pdf\" target=\"_blank\"\u003eManual_P520 V23.1\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003ePurchase Help\u003c\/h2\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/b8a2a61f1ed241a5ada61e12cf731c35.png?v=1765792300\"\u003e\u003c\/p\u003e\n\u003c!----\u003e","brand":"Vazyme","offers":[{"title":"15×1ml","offer_id":44176146596021,"sku":"P520-03","price":897.0,"currency_code":"USD","in_stock":true},{"title":"5×1ml","offer_id":44176146628789,"sku":"P520-02","price":320.0,"currency_code":"USD","in_stock":true},{"title":"1ml","offer_id":44176146661557,"sku":"P520-01","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1780083306473254913.jpg?v=1732864515"},{"product_id":"mag-beads-gst-fusion-protein-purification","title":"Mag-Beads GST Fusion Protein Purification","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"desc-box\"\u003e\n      \u003cp style=\"margin:0;\"\u003eMag-Beads GST fusion protein purification magnetic beads are a new type of purification material developed for purifying GST labeled fusion proteins. They can directly purify high-purity target proteins from biological samples through a unique magnetic separation method, greatly simplifying the purification process and improving purification efficiency. They are suitable for convenient purification of GST fusion proteins in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specification --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table w-640\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n            \u003cth\u003eSpecification\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003e10 ML（10%V\/V)\/25 ML（10%V\/V)\/100 ML（10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eMag-Beads GST Fusion Protein Purification\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComposition\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eSubstrate\u003c\/td\u003e\n            \u003ctd\u003eMagnetic agarose microspheres\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eAverage Particle Size\u003c\/td\u003e\n            \u003ctd\u003e0\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eParticle Size\u003c\/td\u003e\n            \u003ctd\u003e30 μm~150 μm\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eProtein Binding Ability\u003c\/td\u003e\n            \u003ctd\u003e6-10 mg GST fusion protein\/ml (pure magnetic beads)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n            \u003ctd\u003e20% ethanol\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eMagnetic Bead Ratio\u003c\/td\u003e\n            \u003ctd\u003eMagnetic beads suspended in protective solution with a content of 10% (V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComponent\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eFeatures\u003c\/h2\u003e\n    \u003cul\u003e\n      \u003cli\u003eDirectly purifying crude protein reduces equipment, manpower, and time costs, and is more suitable for large-scale protein purification production.\u003c\/li\u003e\n      \u003cli\u003eThe purity and loading of one-step purification are higher than those of ordinary column chromatography.\u003c\/li\u003e\n      \u003cli\u003eGood stability, not affected by flow velocity.\u003c\/li\u003e\n      \u003cli\u003eReusable.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eSuitable for convenient purification of GST fusion proteins in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003e2~8°C, do not freeze\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ml(10%V\/V)","offer_id":52400869802165,"sku":"P310135-10ml(10%V\/V)","price":148.0,"currency_code":"USD","in_stock":true},{"title":"25ml(10%V\/V)","offer_id":52400869834933,"sku":"P310135-25ml(10%V\/V)","price":302.0,"currency_code":"USD","in_stock":true},{"title":"100ml(10%V\/V)","offer_id":52400869867701,"sku":"P310135-100ml(10%V\/V)","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_magbeads.png?v=1751472361"},{"product_id":"mag-beads-his-tag-protein-purification","title":"Mag-Beads His-Tag Protein Purification","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Helvetica Neue', Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.6;\n}\n\n.biofargo-wrap .sec{\n  margin-bottom:30px;\n}\n\n.biofargo-wrap .sec h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .desc-box{\n  background:#f9f9f9;\n  padding:20px;\n  border-radius:6px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap .card{\n  border:1px solid #e1e1e1;\n  padding:20px;\n  border-radius:10px;\n  background:#fff;\n}\n\n\/* =========================\n   Table SOP\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  -webkit-overflow-scrolling:touch;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n  margin:16px 0;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n  white-space:normal;\n  text-align:left;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n.biofargo-wrap table.w-640{\n  min-width:640px;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n  margin:0;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"desc-box\"\u003e\n      \u003cp style=\"margin:0;\"\u003eMag-Beads His Tag protein purification magnetic beads are a new type of purification material developed for purifying His-tag proteins. They can directly purify high-purity target proteins from biological samples through a unique magnetic separation method, greatly simplifying the purification process and improving purification efficiency. They are suitable for convenient purification of His-tag proteins in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specification --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table w-640\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:40%\"\u003eParameter\u003c\/th\u003e\n            \u003cth\u003eSpecification\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003e10 ML（10%V\/V)\/25 ML（10%V\/V)\/100 ML（10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFull name\u003c\/td\u003e\n            \u003ctd\u003eMag-Beads His-Tag Protein Purification\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComposition\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eSubstrate\u003c\/td\u003e\n            \u003ctd\u003eMagnetic agarose microspheres\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eAverage Particle Size\u003c\/td\u003e\n            \u003ctd\u003e0\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eParticle Size\u003c\/td\u003e\n            \u003ctd\u003e30 μm~150 μm\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eProtein Binding Ability\u003c\/td\u003e\n            \u003ctd\u003e\u0026gt;10 mg histidine labeled protein\/ml (pure magnetic beads)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStorage Buffer\u003c\/td\u003e\n            \u003ctd\u003e20% ethanol\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eMagnetic Bead Ratio\u003c\/td\u003e\n            \u003ctd\u003eMagnetic beads suspended in protective solution with a content of 10% (V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eComponent\u003c\/td\u003e\n            \u003ctd\u003e10 ml (10%V\/V)\/ 25 ml (10%V\/V)\/100 ml (10%V\/V)\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eFeatures\u003c\/h2\u003e\n    \u003cul\u003e\n      \u003cli\u003eDirectly purifying crude protein reduces equipment, manpower, and time costs, and is more suitable for large-scale protein purification production.\u003c\/li\u003e\n      \u003cli\u003eOne step purification can obtain up to 95% purity of the target protein.\u003c\/li\u003e\n      \u003cli\u003eGood stability, not affected by flow velocity.\u003c\/li\u003e\n      \u003cli\u003eReusable.\u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eSuitable for convenient purification of His-tag protein in scientific research and industrial fields.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"sec\"\u003e\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"card\"\u003e\n      \u003cp style=\"margin:0;\"\u003e2~8°C, do not freeze\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"10ml(10%V\/V)","offer_id":52400870097077,"sku":"P310137-10ml(10%V\/V)","price":148.0,"currency_code":"USD","in_stock":true},{"title":"25ml(10%V\/V)","offer_id":52400870129845,"sku":"P310137-25ml(10%V\/V)","price":302.0,"currency_code":"USD","in_stock":true},{"title":"100ml(10%V\/V)","offer_id":52400870162613,"sku":"P310137-100ml(10%V\/V)","price":866.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_magbeads.png?v=1751472361"},{"product_id":"2x-taq-pcr-mix","title":"2X Taq PCR Master Mix (with Dye)","description":"\u003cstyle\u003e\n\/* =========================\n   Biofargo Product Page SOP\n========================== *\/\n\n.biofargo-wrap{\n  max-width:1000px;\n  margin:0 auto;\n  padding:10px;\n  font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n  color:#333;\n  line-height:1.7;\n}\n\n.biofargo-wrap .biofargo-sec{\n  margin-bottom:32px;\n}\n\n.biofargo-wrap .biofargo-h2{\n  border-left:4px solid #0056b3;\n  padding-left:15px;\n  color:#0056b3;\n  font-size:20px;\n  text-transform:uppercase;\n  margin:0 0 15px 0;\n}\n\n.biofargo-wrap .biofargo-card{\n  background:#f9f9f9;\n  padding:18px;\n  border-radius:10px;\n  border:1px solid #eee;\n}\n\n.biofargo-wrap ul{\n  padding-left:22px;\n}\n\n.biofargo-wrap li{\n  margin:8px 0;\n}\n\n\/* =========================\n   Table Style\n========================== *\/\n\n.biofargo-wrap .biofargo-table-wrap{\n  overflow-x:auto;\n  border:1px solid #eee;\n  border-radius:10px;\n  padding:6px;\n  background:#fff;\n}\n\n.biofargo-wrap table.biofargo-table{\n  width:100%;\n  border-collapse:collapse;\n  font-size:14px;\n  min-width:640px;\n}\n\n.biofargo-wrap table.biofargo-table th,\n.biofargo-wrap table.biofargo-table td{\n  border:1px solid #ddd;\n  padding:12px;\n  vertical-align:middle;\n  word-break:break-word;\n}\n\n.biofargo-wrap table.biofargo-table thead th{\n  background:#f2f2f2;\n  font-weight:700;\n}\n\n.biofargo-wrap table.biofargo-table tbody td:first-child{\n  font-weight:700;\n  background:#f8f9fa;\n}\n\n\/* =========================\n   Document Button\n========================== *\/\n\n.biofargo-doc-btn{\n  display:inline-block;\n  padding:10px 18px;\n  background:#0056b3;\n  color:#fff !important;\n  border-radius:6px;\n  text-decoration:none;\n  font-weight:600;\n}\n\n.biofargo-doc-btn:hover{\n  background:#003f85;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n\u003c!-- Background --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eBackground\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\"\u003e\n\n\u003cp\u003eThe 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays.\u003c\/p\u003e\n\n\u003cp\u003eThe added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Specification --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\n\u003ctable class=\"biofargo-table\"\u003e\n\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width:40%\"\u003eItem\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003e2X Taq PCR Master Mix (with Dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003ePackage Size\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eConventional PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003e2X SanTaq PCR Mix (with bromophenol blue dye)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eTaq Polymerase Source\u003c\/td\u003e\n\u003ctd\u003eRecombinant, E. coli Expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e25 μg \/ 500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eColor\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e−20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReady-to-Use 2X Formulation\u003c\/strong\u003e\u003cbr\u003e\nPre-mixed with Taq DNA polymerase, MgCl₂, dNTPs, PCR buffer, and enhancers for fast PCR setup.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eDirect Gel Loading\u003c\/strong\u003e\u003cbr\u003e\nContains bromophenol blue dye, enabling PCR products to be loaded directly onto agarose gels.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eReliable Amplification Performance\u003c\/strong\u003e\u003cbr\u003e\nOptimized buffer system supports stable and efficient amplification in routine PCR assays.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eTA Cloning Compatible\u003c\/strong\u003e\u003cbr\u003e\nGenerates PCR products with 3′-dA overhangs, facilitating efficient ligation into T-vectors.\n\u003c\/li\u003e\n\n\u003cli\u003e\n\u003cstrong\u003eEnhanced Stability\u003c\/strong\u003e\u003cbr\u003e\nFormulated with stabilizers; can be stored at 4 °C for up to 3 months and long-term at −20 °C.\n\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\n\u003cul\u003e\n\u003cli\u003eConventional PCR amplification\u003c\/li\u003e\n\u003cli\u003eGene cloning and insert preparation\u003c\/li\u003e\n\u003cli\u003eGenotyping and mutation analysis\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for DNA sequencing\u003c\/li\u003e\n\u003cli\u003eRoutine molecular biology research workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Storage --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n\n\u003cdiv class=\"biofargo-card\" style=\"background:#fff;\"\u003e\n\u003cp\u003eStore the 2X master mix at -20°C.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- Documents --\u003e\n\u003cdiv class=\"biofargo-sec\"\u003e\n\n\u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n\n\u003ca class=\"biofargo-doc-btn\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2X_Taq_PCR_Master_Mix_with_Dye.pdf?v=1766022843\" target=\"_blank\"\u003e\n2X Taq PCR Master Mix (with Dye)\n\u003c\/a\u003e\n\n\u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"KleanAB","offers":[{"title":"1ml","offer_id":52400976887989,"sku":"P310149-1ml","price":21.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_taqpcrmix.jpg?v=1751472348"},{"product_id":"extraction-free-1-step-2x-rt-pcr-master-mix-sybr-green-dye","title":"Extraction-Free 1-Step 2X RT-PCR Master Mix, SYBR Green Dye (#142)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142) enables real-time qualitative and quantitative RT-PCR directly from cultured cells without the need for RNA purification. This 2X premixed solution contains all necessary components for one-step RT-PCR except gene-specific primers and RNA templates.\u003c\/p\u003e\n\u003cp\u003eIt streamlines gene expression analysis by allowing direct amplification from crude lysates, eliminating time-consuming and laborious RNA purification steps.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-step RT-qPCR workflow: reverse transcription and PCR amplification combined in a single tube.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRobust performance: supports 10 to 100,000 cells per sample, results comparable to purified RNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEconomic and efficient: \u003cbr\u003e2 × 1 mL packaging—200 reactions at 20 μL or 400 reactions at 10 μL final volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSYBR Green detection method.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompatible with most real-time PCR instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTry Before You Buy — \u003cstrong\u003eRequest a trial sample.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eWhy Choose Biofargo #142?\u003c\/h3\u003e\n\u003cp\u003eOur Extraction-Free 1-Step RT-PCR Master Mix, SYBR Green Dye (#142), is designed as a cost-effective alternative to Thermo Fisher's Cells-to-CT™ 1-Step Power SYBR™ Green Kit.\u003c\/p\u003e\n\u003cp\u003eWith comparable performance in gene expression analysis directly from cell lysates, our kit offers reaction costs as low as $4 per reaction—ideal for both research laboratories and high-throughput applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003eThe 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/142_UM_Extraction_Free_Master_Mix_SYBR_Green_07152024_1_2.pdf?v=1758091750\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" target=\"_blank\"\u003e\u003cstrong\u003eExtraction-free RT-PCR from cultured cells\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44624578314421,"sku":"RT-PCR-142-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44624578347189,"sku":"RT-PCR-142-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44624578379957,"sku":"RT-PCR-142-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44624578412725,"sku":"RT-PCR-142-LowRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44624578445493,"sku":"RT-PCR-142-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44624578478261,"sku":"RT-PCR-142-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44624578511029,"sku":"RT-PCR-142-HighRox-2","price":371.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44624578543797,"sku":"RT-PCR-142-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44624578576565,"sku":"RT-PCR-142-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133256.jpg?v=1732871896"},{"product_id":"extraction-free-rt-pcr-master-mix-taqman-probe-149","title":"1-Step 2X Super multiplex RT-PCR Master Mix-TaqMan Probe (#149)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eThis one-step 2× multiplex RT-PCR Master Mix is designed for real-time qualitative and quantitative amplification with TaqMan probes directly from extraction-free RNA samples. The 2× premixed solution provides all necessary components except gene-specific primers, TaqMan probes, and RNA templates. RT-PCR 149 is optimized for high-GC templates and stem-loop rich regions, and supports multiplex detection of up to two targets, ensuring reliable reverse transcription and amplification of challenging RNA templates.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eKit Characteristics\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment, versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-rte-list=\"default\"\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe master mix contains extra more \u003c\/span\u003eredundant amounts of the RTase and DNA polymerase,\u003cspan\u003e specifically designed to overcome endogenous inhibition in extraction-free RNA samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe RTase is easily heat-inactivated at ≥90\u003c\/span\u003e°\u003cspan\u003eC for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer,\u003cem\u003e ≤1 unit per 20μL of reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe kit also contains\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTaq\u003c\/em\u003e-Fast DNA polymerase which extends more than 300 bases with short cycling program.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (Table 1)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eHarvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHeat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRun RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/149-UM_Super_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091748\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2x1ml","offer_id":44628592099509,"sku":"RT-PCR-149-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5x1ml","offer_id":44628592132277,"sku":"RT-PCR-149-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 1x10ml","offer_id":44628592165045,"sku":"RT-PCR-149-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2x1ml","offer_id":44628592197813,"sku":"RT-PCR-149-LowRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5x1ml","offer_id":44628592230581,"sku":"RT-PCR-149-LowRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 1x10ml","offer_id":44628592263349,"sku":"RT-PCR-149-LowRox-10","price":1854.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2x1ml","offer_id":44628592296117,"sku":"RT-PCR-149-HighRox-2","price":462.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5x1ml","offer_id":44628592328885,"sku":"RT-PCR-149-HighRox-5","price":927.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 1x10ml","offer_id":44628592361653,"sku":"RT-PCR-149-HighRox-10","price":1854.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a9625ef0e407146a060016379075c19d.jpg?v=1757990155"},{"product_id":"cat-rt-pcr-146-1-step-2x-multiplex-rt-pcr-master-mix-taqman-probe","title":"Extraction-Free 1-Step 2X Multiplex RT-PCR Master Mix-TaqMan Probe (#146)-Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe 1-Step 2X Multiplex RT-PCR Master Mix is used for real-time qualitative and quantitative multiplex RT-PCR amplifications with TaqMan probes for up to four templates. \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and RNA templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Characteristics\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDirect-from-sample: enables expression analysis directly from cultured cells without RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFast preparation: 5-minute lysis including DNase treatment versus 30–60 minutes for traditional RNA purification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit is designed for multiplex RT-PCR with TaqMan probes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eFor the reverse transcription step, this kit uses a highly efficient Thermophilic Reverse Transcriptase (US patent pending), which is a thermophilic type A polymerase, with optimal temperatures of 60-62°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase is easily heat-inactivated at ≥90°C for 1min.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase efficiently synthesizes complementary DNA strands on RNA templates from gene-specific primers at ≥1 unit per 20 μL of reaction.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe RTase reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit also contains Taq-Probe DNA polymerase, specially engineered for TaqMan probes and generating S-shaped curves.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eUp to four pairs of gene-specific primers can be applied in one reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe concentrations of the primers and probes are variable depending on assay designs and thermocycling protocols (User Manual Table 1).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe preferable PCR product size is ≤150bp.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit has three formulations of ROX, Low ROX or High ROX concentration for your choice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e2X Master Mix (2 × 1 mL \/ 5 × 1 mL \/ 10 mL for 200, 500, or 1000 × 20 μL reactions).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eLysis Buffer (2 × 10 mL \/ 1 × 50 mL \/ 1 × 100 mL).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eUser Manual.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eStore at -20°C (stable up to 24 months with ≤10 freeze-thaw cycles). Transport at ≤4°C for up to 3 days.\u003c\/p\u003e\n\u003ch2\u003eReaction Setup\u003c\/h2\u003e\n\u003cp\u003e20 μL Reaction: 10 μL Master Mix, primers, ≤4 μL RNA sample, water to 20 μL.\u003cbr\u003e10 μL Reaction: 5 μL Master Mix, primers, ≤2 μL RNA sample, water to 10 μL.\u003cbr\u003eRecommended amplicon size: ≤150 bp.\u003c\/p\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eCompatible with all major real-time PCR instruments, including but not limited to Applied Biosystems®, Bio-Rad®, Roche®, Qiagen®, Eppendorf®, and more.\u003c\/p\u003e\n\u003ch2\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Genetic_Tools_Making_RNA_Test_as_Easy_as_DNA_600x600.jpg?v=1746691753\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e1.Harvest 10,000–100,000 cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2.Add lysis buffer and incubate for 5 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e3.Heat at 95°C for 10 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e4.Add lysate directly to RT-PCR setup.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5.Run RT-qPCR and analyze results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eProduct Comparison\u003c\/h2\u003e\n\u003cp\u003e142: SYBR Green, No multiplexing, No probe system, Standard single-gene expression.\u003c\/p\u003e\n\u003cp\u003e146: TaqMan, Yes multiplexing, Yes probe system, Multiplex TaqMan assays.\u003c\/p\u003e\n\u003cp\u003e149: TaqMan, Yes (Super Multiplex), Yes probe system, Highly complex multi-target detection.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3 data-start=\"62\" data-end=\"154\"\u003eQ: What nucleotides are included in the 2× Probe qPCR Master Mix? Does it contain UTP?\u003c\/h3\u003e\n\u003cp data-start=\"155\" data-end=\"654\"\u003e\u003cstrong data-start=\"155\" data-end=\"161\"\u003eA:\u003c\/strong\u003e The 2× Probe qPCR Master Mix contains all standard deoxynucleotides—dATP, dTTP, dGTP, and dCTP—each at a standard concentration of 200 µM.\u003cbr data-start=\"308\" data-end=\"311\"\u003eUTP is not included by default, but custom formulations with UTP can be provided on request, depending on the required quantitative range.\u003cbr data-start=\"457\" data-end=\"460\"\u003eAdditionally, the mix uses a next-generation hot-start enzyme, which allows for higher reaction temperatures and results in enhanced dNTP stability and overall amplification performance.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/146-UM_Multiplex_Master_Mix_TaqMan_07282023_2.pdf?v=1758091746\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eUser Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Extraction_free_RT-PCR_07242025_1.pdf?v=1758621950\" type=\"application\/pdf\" target=\"_blank\"\u003eExtraction-free RT-PCR from cultured cells\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"No ROX \/ 2X 1mL","offer_id":44869815828661,"sku":"RT-PCR-146-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 5X 1mL","offer_id":44869815861429,"sku":"RT-PCR-146-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"No ROX \/ 10mL","offer_id":44869815894197,"sku":"RT-PCR-146-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 2X 1mL","offer_id":44869815926965,"sku":"RT-PCR-146-LowRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 5X 1mL","offer_id":44869815959733,"sku":"RT-PCR-146-LowRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"Low ROX \/ 10mL","offer_id":44869815992501,"sku":"RT-PCR-146-LowRox-10","price":1976.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 2X 1mL","offer_id":44869816025269,"sku":"RT-PCR-146-HighRox-2","price":421.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 5X 1mL","offer_id":44869816058037,"sku":"RT-PCR-146-HighRox-5","price":1027.0,"currency_code":"USD","in_stock":true},{"title":"High ROX \/ 10mL","offer_id":44869816090805,"sku":"RT-PCR-146-HighRox-1","price":1976.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20240911-133240_1_1.jpg?v=1733237326"},{"product_id":"rt-easytm-ii-master-premix-for-first-strand-cdna-synthesis-for-real-time-pcr","title":"RT EasyTM II (Master Premix for first-strand cDNA synthesis for Real Time PCR)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe company's 2× RT OR-EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.\u003c\/p\u003e\n\u003cp\u003eThe RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. Foregene 2× RT Mix shows extremely high tolerance to alcohol and guanidine salt. The highest tolerance to alcohol in RNA samples is 60%, and the highest tolerance to guanidine salt is 750mM. Even impure RNA can be used for reverse transcription reaction with Foregene 2× RT OR-EasyTM Mix.\u003c\/p\u003e\n\u003cp\u003e2× RT OR-EasyTM Mix is a rapid reverse transcription reagent specially developed for Real Time PCR. This system has high reverse transcription efficiency and does not need to add any primers, and can perform good reverse transcription reactions on a small amount of RNA templates. The unique reaction system makes the RT reaction easier, faster and more efficient. The first strand cDNA synthesis can be completed in 20 minutes. This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003eKit components\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-01022\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRT-01023\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e200 T(10μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e800 T (10μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× RT OR-EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1ml × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.7ml × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eQuick and efficient\u003cbr\u003e\u003c\/strong\u003eEfficient reverse transcription system, it only takes 20 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-sensitivity \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh thermal stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComprehensive\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEven impure RNA samples (alcohol up to 60%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2× RT OR-EasyTM Mix has added optimized reverse transcription primers (Random Primer, Oligo(dT)18 Primer).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReal Time PCR\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUsed directly in Real Time PCR to quantitatively analyze gene expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAnalyze small amounts of RNA\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIt can quickly and accurately analyze small amounts of RNA such as RNA viruses\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComplex system\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReverse transcription of RNA templates with high GC content or complex secondary structure\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTransportation conditions\u003cbr\u003e\u003c\/strong\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4°C.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStorage conditions\u003cbr\u003e\u003c\/strong\u003eRT EasyTM II is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_II_Instruction_Manual-V1.0-EN.docx?v=1752836168\"\u003e\u003cstrong\u003eRT Easy™ II Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"200×10μl Reactions","offer_id":51360747716789,"sku":"RT-01022","price":278.0,"currency_code":"USD","in_stock":true},{"title":"800×10μl Reactions","offer_id":51360747749557,"sku":"RT-01023","price":1047.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-010_1_f10f54a7-b7ad-4690-98be-b18fb34f6273.jpg?v=1752810994"},{"product_id":"master-premix-for-first-strand-cdna-synthesis-for-real-time-pcr-with-gdnase","title":"Master Premix for first-strand cDNA synthesis for Real Time PCR with gDNase","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRT Easy II(with gDNase) Master Premix system of this kit can also maintain good activity at 50°C. Therefore, it is more advantageous for complex RNA templates and can easily transcribe RNA templates with high GC content and complex secondary structure.\u003c\/p\u003e\n\u003cp\u003eRT EasyTM II (With gDNase) is a reverse transcription system specially developed for Real Time PCR to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.\u003c\/p\u003e\n\u003cp\u003e5× RO-EasyTM Mix contains Foregene Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase with stronger RNA affinity. The optimized system makes the reverse transcription rate faster, and the first strand cDNA synthesis can be completed at 42°C in 15 minutes\u003c\/p\u003e\n\u003ch2\u003eKit components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e5×gDNase Mix\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5×RO-EasyTM Mix\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRNase-Free ddH2O\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInstructions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eComplex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eUsed directly in Real Time PCR to quantitatively analyze gene expression.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIt can quickly and accurately analyze trace RNA such as RNA virus.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReverse transcription of RNA templates with high GC content or complex secondary structure.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThe kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_II_With_gDNase_Instruction_Manual-V1.0.docx?v=1752835294\"\u003e\u003cstrong\u003eRT Easy™ II (With gDNase) Instruction Manual-V1.0\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"100×20μl Reactions","offer_id":51360760627381,"sku":"RT-01032","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT-010_4_40340e9a-2557-4ad4-9741-6b1d6ea540c8.jpg?v=1752812181"},{"product_id":"rt-easytm-i-master-premix-for-first-strand-cdna-synthesis","title":"RT EasyTM I (Master Premix for first-strand cDNA synthesis)","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eFOREGENE’s 2× RT EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.\u003c\/p\u003e\n\u003cp\u003eThe RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT EasyTM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.\u003c\/p\u003e\n\u003cp\u003eThis product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.\u003c\/p\u003e\n\u003ch2\u003eProduct components\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eKit contents\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-01011\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRT-01012\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e25 times (20μl system)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100 times (20μl system)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2× RT EasyTM Mix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e0.25ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRandom Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eOligo(dT)18 Primer (50μM)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e50μl\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e200μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1.7ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eInstructions Manual\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient reverse transcription system, it only takes 25 minutes to complete the synthesis of the first strand cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEven impure RNA samples (alcohol up to 45%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eConventional RT-PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSynthesis of cDNA for cloning and expression research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAnalysis of the primer extension method of the transcription initiation site\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRACE (rapid amplification of cDNA ends)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLinear RNA amplification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChip mark\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePreparations before operation\u003c\/h2\u003e\n\u003cp\u003eIt is strongly recommended that users read the instructions carefully before using this kit. The RT Easy series kits are simple, convenient and fast to operate. The instructions provide the correct use of the entire kit. Please prepare necessary experimental materials and equipment before use.\u003c\/p\u003e\n\u003ch2\u003eStorage and Shelf life\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe whole process of low-temperature ice box transportation, to ensure that the kit is in a state of \u0026lt;4 ℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RT_Easy_I_Instruction_Manual-V1.0-EN.docx?v=1752834757\"\u003e\u003cstrong\u003eRT Easy™ I Instruction Manual-V1.0-EN\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Foregene","offers":[{"title":"25× 20μl Reactions","offer_id":51366533857461,"sku":"RT-01011","price":188.0,"currency_code":"USD","in_stock":true},{"title":"100 × 20μl Reactions","offer_id":51366533890229,"sku":"RT-01012","price":643.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RTEasyTMI_MasterPremixforfirst-strandcDNAsynthesis_01.jpg?v=1752835213"},{"product_id":"thunderbird-probe-qpcr-mix-100-rxn","title":"THUNDERBIRD Probe qPCR Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eTHUNDERBIRD™ Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.\u003cbr\u003eThese products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and Realtime PCR Master Mix (Code No. QPK-201) . In particular, reaction specificity and PCR efficiency is enhanced.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003eThis product is sold in U.S. under the license of US patent 7772383\u003cbr\u003efrom Chakrabarti Advanced Technology NewCo LLC.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The specificity for the detection of low-copy targets is improved.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e High specificity and effective amplification enable the detection of a broad dynamic range.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-101T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1 mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.67 mL × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e250 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-201T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1 mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-201\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.67 mL × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e250 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eCompatible Real-Time Instruments\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 900px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eManufacturer\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eInstrument\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eManufacturer\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eInstrument\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eABI PRISM™ 7000\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ 1.x \/ 2.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eABI PRISM™ 7700\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ Nano\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7300\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ 480\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7500\/7500FAST\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eBio-Rad \/ MJ\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eMiniOpticon™\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7900HT\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eBio-Rad \/ MJ\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eCFX96 Touch™\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ StepOne™\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eAgilent Technologies\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eMx3000P \/ Mx3005P \/ Mx4000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ StepOnePlus™\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTaKaRa\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eThermal Cycler Dice™ Real Time Systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1. Comparison of the sensitivity and efficiency of the SYBR® Green I assay\u003c\/h3\u003e\n\u003cp\u003eβ-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD™ SYBR® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data01.png?v=1775522456\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data02.png?v=1775522455\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data03_pc.png?v=1775522456\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2. Comparison of the PCR efficiency\u003c\/h3\u003e\n\u003cp\u003eNorovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD™ qPCR Master Mix showed greater efficiency than other reagents.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data04.png?v=1775522561\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of Norovirus G1 gene by SYBR® Green I assay.\u003cbr\u003e[Roche Diagnostics LightCycler 1.1]\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data05.png?v=1775522582\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of GAPDH gene by TaqMan™ assay.\u003cbr\u003e[Roche Diagnostics LightCycler 1.1]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3. Verification of the measurement accuracy\u003c\/h3\u003e\n\u003cp\u003eHuman GAPDH genes were detected using serially 20.5 fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD™ qPCR Master Mix successfully detected the differences between dilutions.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data06.png?v=1775522659\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of human GAPDH gene by SYBR® GREEN I assay.\u003cbr\u003e[Applied Biosystems 7900HT]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPS-101.pdf?v=1775800214\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPS-1_MSDS.pdf?v=1775800214\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"100Rxn","offer_id":52417295515829,"sku":"QPS-101T","price":48.32,"currency_code":"USD","in_stock":true},{"title":"500Rxn","offer_id":52417295548597,"sku":"QPS-101","price":200.96,"currency_code":"USD","in_stock":true},{"title":"2500Rxn","offer_id":52417295581365,"sku":"QPS-101X5","price":904.48,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/THUNDERBIRD_Probe_qPCR_Mix-Toyobo.jpg?v=1777517274"},{"product_id":"thunderbird-sybr-qpcr-mix","title":"THUNDERBIRD SYBR qPCR Mix-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eTHUNDERBIRD™ Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.\u003cbr\u003eThese products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and Realtime PCR Master Mix (Code No. QPK-201) . In particular, reaction specificity and PCR efficiency is enhanced.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003eThis product is sold in U.S. under the license of US patent 7772383\u003cbr\u003efrom Chakrabarti Advanced Technology NewCo LLC.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The specificity for the detection of low-copy targets is improved.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e High specificity and effective amplification enable the detection of a broad dynamic range.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20ºC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-101T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1 mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ Probe qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.67 mL × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e250 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-201T\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1 mL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQPS-201\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTHUNDERBIRD™ SYBR® qPCR Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.67 mL × 3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e50x ROX reference dye\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e250 µL × 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible Real-Time Instruments\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 900px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eManufacturer\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eInstrument\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eManufacturer\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eInstrument\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eABI PRISM™ 7000\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ 1.x \/ 2.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eABI PRISM™ 7700\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ Nano\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7300\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRoche Diagnostics\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLightCycler™ 480\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7500\/7500FAST\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eBio-Rad \/ MJ\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eMiniOpticon™\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ 7900HT\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eBio-Rad \/ MJ\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eCFX96 Touch™\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ StepOne™\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eAgilent Technologies\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eMx3000P \/ Mx3005P \/ Mx4000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eApplied Biosystems™ StepOnePlus™\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eTaKaRa\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eThermal Cycler Dice™ Real Time Systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1. Comparison of the sensitivity and efficiency of the SYBR® Green I assay\u003c\/h3\u003e\n\u003cp\u003eβ-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD™ SYBR® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data01.png?v=1775522456\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data02.png?v=1775522455\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data03_pc.png?v=1775522456\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2. Comparison of the PCR efficiency\u003c\/h3\u003e\n\u003cp\u003eNorovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR® Green I and TaqMan real-time PCR. THUNDERBIRD™ qPCR Master Mix showed greater efficiency than other reagents.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data04.png?v=1775522561\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of Norovirus G1 gene by SYBR® Green I assay.\u003cbr\u003e[Roche Diagnostics LightCycler 1.1]\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data05.png?v=1775522582\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of GAPDH gene by TaqMan™ assay.\u003cbr\u003e[Roche Diagnostics LightCycler 1.1]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3. Verification of the measurement accuracy\u003c\/h3\u003e\n\u003cp\u003eHuman GAPDH genes were detected using serially 20.5 fold diluted cDNA synthesised from HeLa cell total RNA by SYBR® Green I assay. THUNDERBIRD™ qPCR Master Mix successfully detected the differences between dilutions.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_application_data06.png?v=1775522659\"\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #666; font-style: italic; margin-top: 10px;\"\u003eDetection of human GAPDH gene by SYBR® GREEN I assay.\u003cbr\u003e[Applied Biosystems 7900HT]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366; padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \u003ca rel=\"noopener noreferrer\" style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"100Rxn","offer_id":52417298202805,"sku":"QPS-201T","price":48.32,"currency_code":"USD","in_stock":true},{"title":"500Rxn","offer_id":52417298235573,"sku":"QPS-201","price":200.96,"currency_code":"USD","in_stock":true},{"title":"2500Rxn","offer_id":52417298268341,"sku":"QPS-201X5","price":904.48,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/THUNDERBIRD_SYBR_qPCR_Mix-Toyobo.jpg?v=1777517231"}],"url":"https:\/\/biofargo.com\/collections\/pcr-master-mix.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}