{"title":"Pathogen Detection Kits","description":"\u003ch1\u003e\u003cstrong\u003ePathogen Detection Kits\u003c\/strong\u003e\u003c\/h1\u003e\n\u003chr style=\"border: none; border-top: 2px solid #ccc; margin: 20px 0;\"\u003e\n\u003cp\u003eEngineered for exceptional sensitivity and specificity, our ready-to-use PCR and qPCR kits are designed to streamline your workflow across diverse applications—from agricultural screening and food safety to biopharmaceutical quality control and basic research.\u003c\/p\u003e\n\u003cp\u003eTo help you quickly locate the right tools for your specific targets, our assay portfolio is strategically categorized into four core analytical platforms:\u003c\/p\u003e\n\u003cp style=\"font: arial;\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e➜ To inquire about our local laboratory pricing, or for bulk inquiries, please email us at contact@biofargo.com\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"font: arial;\"\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e🚫 These products require laboratory authentication. These products are strictly for research use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003chr style=\"border: none; border-top: 2px solid #ccc; margin: 20px 0;\"\u003e\n\u003ch2\u003eExplore Our Featured Collections\u003c\/h2\u003e","products":[{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-vero-dna-size-analysis-kit-2g-shentek™","title":"Residual Vero DNA Size Analysis Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) based assay for rapid and specific quantitation of residual Vero (host cell) DNA fragments across multiple size ranges in biopharmaceutical samples (in-process to final product). The kit is designed to amplify four discrete fragment lengths (85 bp, 134 bp, 229 bp and 552 bp) and determine size distribution and quantity down to the femtogram (fg) level. Includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, DNA dilution buffer (DDB) and a Vero DNA control for generating standard curves. Reagents are provided for 4 × 100 reactions. For sample extraction and preparation refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 134 bp, 229 bp, 552 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReal-time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects and quantifies Vero DNA down to the femtogram (fg) level (standard curve includes concentrations down to 0.03 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit dilution scheme)\u003c\/td\u003e\r\n \u003ctd\u003eST0 (prepared) 3000 pg\/µL; ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL template\/standard\/NTC (total 30 µL reaction volume). qPCR MIX composition per reaction: qPCR Reaction Buffer 15.9 µL; Vero Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL; total qPCR MIX 20 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control (IPC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eSample Ct(IPC) should be within ±1.0 Ct of NCS Ct(IPC). Significant increase indicates potential inhibition; evaluate recovery with ERC samples.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 2 Ct higher than mean Ct of the lowest standard concentration, or laboratory-specific acceptance criteria established during validation.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months; check label expiration date.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation and size-distribution analysis of residual Vero DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-fragment size analysis:\u003c\/strong\u003e Four specific primer\/probe assays amplify 85 bp, 134 bp, 229 bp and 552 bp fragments to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual DNA at femtogram-level sensitivity using absolute quantitation and serial standard curves.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBuilt-in internal control:\u003c\/strong\u003e IPC mix included to monitor inhibition and reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR reaction buffer and primer\/probe mixes minimize setup variability and speed workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus) and adjustable to other qPCR instruments following instrument software guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero (host cell) DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA fragment size distribution during downstream processing and in final product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and release testing in research and development (RUO) workflows\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery studies for host cell DNA assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-85 (NNC020) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-134 (NNC021) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-229 (NNC022) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-552 (NNC023) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProvided documentation\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions total) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read MSDS and user guide before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-decontaminate workspace, use DNase-free consumables, aliquot reagents and change tips between samples.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents as labeled; check expiration date on labels.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific settings and analysis parameters; adjust thresholds\/baselines per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: standards (ST1–ST5), NTC, NCS and IPC for each run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed for femtogram-level sensitivity. The supplied standard curve includes concentrations down to 0.03 pg\/µL (ST5). Exact LOD\/LOQ should be established during laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500 (with ROX), Bio-Rad CFX96 and LineGene 9600plus. Other real-time PCR instruments are supported; follow instrument-specific setup and dye assignments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted and what are acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSet up an absolute quantitation standard curve (ST1–ST5). IPC Ct for samples should be within ±1.0 of the NCS IPC Ct; significant deviation indicates inhibition. NTC Ct should be at least 2 Ct higher than the mean Ct of the lowest standard (or per laboratory acceptance criteria).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw Vero DNA Control and DDB on ice. Prepare ST0 at 3000 pg\/µL using DDB, then perform serial 1:10 dilutions to obtain ST1=300 pg\/µL, ST2=30 pg\/µL, ST3=3 pg\/µL, ST4=0.3 pg\/µL and ST5=0.03 pg\/µL. Include at least five standard points (suggested ST1–ST5) in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189155881141,"sku":"1103174","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Size_Analysis_Kit_2G.jpg?v=1742290678"},{"product_id":"residual-hek293-dna-size-analysis-kit-shentek™","title":"Residual HEK293 DNA Size Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HEK293 DNA Size Analysis Kit is designed to quantify residual HEK293 host cell DNA fragments of different sizes across biopharmaceutical sample types (in-process samples to final products) using real-time PCR (qPCR). The kit amplifies four fragment sizes (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer\/probe mixes for each fragment, internal positive control (IPC), DNA dilution buffer and a HEK293 DNA control for standard curve preparation. For extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eReal-time PCR (qPCR) with TaqMan-style primer \u0026amp; probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned for quantitation at the femtogram (fg) level (lowest standard in kit workflow corresponds to 0.03 pg\/µL = 30 fg)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (prepared from supplied HEK293 DNA Control)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock prior to dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear \/ working range (as provided by recommended standards)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard points from 0.03 pg\/µL to 300 pg\/µL (prepare standard curve using ST1–ST5); ST0 (3000 pg\/µL) used as stock\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR master mix + 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; HEK293 Primer\u0026amp;Probe MIX (appropriate fragment) 2.8 μL; IPC MIX 1.3 μL; total 20 μL (then add 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/read: 72 °C 01:30; 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, FQD-96A and similar real-time PCR instruments (user must configure detectors\/channels accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC \/ quality control criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. Ct of NTC and NCS should be larger than the mean Ct of the lowest concentration standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability \/ shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Simultaneous assays for four discrete fragment lengths (75, 122, 244, 562 bp) allow determination of residual HEK293 DNA size distribution.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify DNA down to the femtogram range (lowest recommended standard 0.03 pg\/µL = 30 fg).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated HEK293 Primer\u0026amp;Probe MIXes for each fragment size reduce setup time and minimize variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control (IPC):\u003c\/strong\u003e IPC MIX included to monitor inhibition and assay performance; acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible instrument compatibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (instructions include detector\/channel setup and reference dye settings).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA dilution buffer and HEK293 DNA control for standard curve preparation; refers to complementary sample prep kit for extraction.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical process and final product testing\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and process development for biologics produced in HEK293 cells\u003c\/li\u003e\r\n \u003cli\u003eRelease testing and quality control (research-use contexts)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-75 (NNC012), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-122 (NNC013), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-244 (NNC014), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-562 (NNC015), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after thaw; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control (NNA005), 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment-specific primer\/probe mix (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points (recommended ST1–ST5) and run samples in triplicate where possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR run setup: reaction volume 30 μL (20 μL master mix + 10 μL template); thermal cycling and detector settings must be configured per instrument.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: sample Ct-IPC should be within ±1.0 of NCS Ct-IPC — higher Ct-IPC indicates potential inhibition; consider recovery controls.\u003c\/p\u003e\r\n \u003cp\u003e• Negative controls (NTC) and NCS should show Ct values greater (later) than the mean Ct of the lowest standard point.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity \/ limit of detection (LOD) of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to quantify HEK293 DNA at the femtogram level. The supplied standard curve includes points down to 0.03 pg\/µL (30 fg); actual LOD\/LOQ should be established during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which fragment sizes are measured and how are they assayed?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFour fragment sizes are assayed separately using fragment-specific primer \u0026amp; probe mixes: 75 bp, 122 bp, 244 bp and 562 bp. Prepare four separate standard curves (one per fragment) and run each assay on the qPCR instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems such as SHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, and FQD-96A. Users must configure detectors\/channels, passive reference dye (e.g., ROX if required), and cycling conditions according to their instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibited?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (internal positive control). The sample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. A significantly higher Ct-IPC in the sample suggests inhibition; dilute the sample or perform recovery controls and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189170954421,"sku":"1103176","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Size_Analysis_Kit.jpg?v=1742291951"},{"product_id":"mycobacteria-dna-detection-kit-mycoshentek™","title":"Mycobacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK® Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, and similar materials using real-time qPCR technology. The integrated system has been validated with a limit of detection of 10–100 CFU\/mL and can detect more than 100 different Mycobacteria species. The assay chemistry includes dUTP and is compatible with UNG (uracil-N-glycosylase) treatment to prevent carry-over contamination. An Internal Control (IC) is provided to monitor PCR inhibition and\/or extraction efficiency. The kit is supplied with positive and negative controls and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd\u003e1503602\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit Format \/ Reactions\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 Reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQualitative detection of Mycobacteria contamination in cells and cell culture-derived biologicals\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated at 10–100 CFU\/mL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n \u003ctd\u003eValidated to detect \u0026gt;100 Mycobacteria species\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay type\u003c\/td\u003e\r\n \u003ctd\u003eReal-time qPCR (probe-based)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL (10 µL qPCR MIX + 20 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eMB qPCR Reaction Buffer 8 µL; MB Primer \u0026amp; Probe MIX 1.5 µL; Internal Control (IC) 0.5 µL; total 10 µL. UNG optional (0.1 U per reaction).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG 25 °C 10:00 (1 cycle); Activation 95 °C 10:00 (1 cycle); 45 cycles of Denaturation 95 °C 00:15, Annealing 60 °C 00:30, Extension 72 °C 01:30 (read fluorescence during extension).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls\u003c\/td\u003e\r\n \u003ctd\u003ePositive Control (PC), No Template Control (NTC), Negative Control Sample (NCS), Internal Control (IC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\r\n \u003ctd\u003eTypical interpretation: FAM positive cutoff Ct \u0026lt;40.00 (positive if at least one well Ct\u0026lt;40 and normal amplification); VIC (IC) expected Ct \u0026lt;35.00 for normal amplification; PC Ct \u0026lt;35.00. NTC FAM Ct ≥40.00 or no amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, and other real-time PCR systems supporting probe detection\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Validated limit of detection down to 10–100 CFU\/mL for broad Mycobacteria detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed and validated to detect over 100 different Mycobacteria species.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarry-over contamination control:\u003c\/strong\u003e Chemistry includes dUTP and is compatible with UNG treatment to prevent and eliminate carry-over contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IC):\u003c\/strong\u003e IC monitors PCR inhibition and\/or extraction efficiency; can be added pre-extraction or to the PCR mix.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-validated workflow:\u003c\/strong\u003e Includes positive and negative controls and recommended plate layouts and acceptance criteria for straightforward result interpretation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQualitative detection of Mycobacteria contamination in cell lines\u003c\/li\u003e\r\n \u003cli\u003eScreening of cell culture-derived biologicals for Mycobacteria contamination\u003c\/li\u003e\r\n \u003cli\u003eRoutine mycoplasma\/contaminant monitoring workflows in research laboratories (Mycobacteria-specific)\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of Mycobacteria DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB qPCR Reaction Buffer (NNB011)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light; 400 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Primer \u0026amp; Probe MIX (NNC035)\u003c\/span\u003e\u003cstrong\u003e2–8 °C until use or -20 °C; 75 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Internal Control (IC) (NNA037)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or -20 °C; 600 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Positive Control (PC) (NNA041)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or room temperature per label; 1.5 mL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use UV decontamination (30 minutes) and 75% ethanol to clean surfaces and equipment before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use UNG enzyme optionally to reduce carry-over contamination (check effective amount before use).\u003c\/p\u003e\r\n \u003cp\u003e• If VIC (IC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout and include PC, NTC and NCS as described; use duplicates for samples and controls.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe integrated system has been validated with a limit of detection of approximately 10–100 CFU\/mL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the kit detect multiple Mycobacteria species?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The assay has been validated to detect more than 100 different Mycobacteria species.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is an Internal Control provided and how should it be used?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAn Internal Control (IC) is provided. It can be added during sample extraction to monitor extraction efficiency or added to the PCR mix to monitor PCR inhibition. If IC was added during extraction, replace IC volume with DNA dilution buffer when preparing the qPCR mix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended instruments and reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500). Prepare 30 µL reaction volumes (10 µL qPCR MIX + 20 µL sample\/control) and run the recommended cycling program with UNG treatment if used.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"Default Title","offer_id":43795617874101,"sku":"1503602","price":1525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Detection_Kit.jpg?v=1742292467"},{"product_id":"mycoplasma-dna-detection-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20°C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR-based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culture–derived products. The kit is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII, with a stated limit of detection of 10 CFU\/mL. The assay detects \u0026gt;200 species with high specificity and includes Internal Control (IC, 2G) to monitor PCR inhibition and optionally to evaluate extraction efficiency when added prior to extraction. The kit is supplied for 50 reactions and is intended for research use only (RUO).\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eAssay type\u003c\/td\u003e\n \u003ctd\u003eQualitative real-time PCR (qPCR) assay\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\n \u003ctd\u003eDetects \u0026gt;200 species of Mycoplasma, Spiroplasma and Acholeplasma\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls included\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eInternal Control (IC) (2G), Positive Control (PC) (2G)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction format \u0026amp; volume\u003c\/td\u003e\n \u003ctd\u003e30 μL total reaction volume: 10 μL qPCR MIX + 20 μL sample (per well)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR Mix composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eMy qPCR Reaction Buffer (2G) 8 μL; My Primer \u0026amp; Probe MIX (2G) 1.5 μL; Internal Control (IC) (2G) 0.5 μL (total 10 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n \u003ctd\u003eUNG 25°C 10:00 (1); Activation 95°C 10:00 (1); 45 cycles of: Denaturation 95°C 00:15, Annealing 62°C 00:30, Extension 72°C 01:30 (fluorescence read during extension)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection channels \/ dyes\u003c\/td\u003e\n \u003ctd\u003eTarget: FAM; Internal Control: VIC; ROX optional as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, Roche LightCycler 480 and other real-time PCR systems\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended sample types \/ matrices\u003c\/td\u003e\n \u003ctd\u003eMaster cell bank, working cell bank, virus seed stock, cell culture-derived products and similar matrices (validated across a variety of matrices)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n \u003ctd\u003e-20°C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReconstitution instructions (controls)\u003c\/td\u003e\n \u003ctd\u003eReconstitute lyophilized IC (2G) in 600 μL DNA Dilution Buffer; reconstitute lyophilized PC (2G) in 500 μL DNA Dilution Buffer\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\n \u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35; PC: FAM Ct \u0026lt; 35; Unknown positive: FAM Ct \u0026lt; 40 (≥1 well) with normal amplification; If VIC inhibited, repeat extraction or remove inhibitors\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated LOD of 10 CFU\/mL allowing detection of low-level mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed to detect more than 200 Mycoplasma, Spiroplasma and Acholeplasma species with high specificity\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition and extraction monitoring:\u003c\/strong\u003e Internal Control (IC, 2G) can be added to PCR or to the sample prior to extraction to monitor PCR inhibition and evaluate extraction efficiency\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeia standards:\u003c\/strong\u003e Validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Provided My Primer \u0026amp; Probe MIX (2G) simplifies assay setup; lyophilized positive and internal controls included\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (SHENTEK-96S, CFX96, 7500, LightCycler 480, etc.)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQualitative mycoplasma testing of master cell banks and working cell banks\u003c\/li\u003e\n \u003cli\u003eScreening of virus seed stocks for mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture–derived products and biologics for mycoplasma\u003c\/li\u003e\n \u003cli\u003eRoutine cell culture contamination monitoring and QC in R\u0026amp;D and production (research use only)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eInternal Control (IC) (2G) (Part No. NNA035) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 600 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePositive Control (PC) (2G) (Part No. NNA039) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 500 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy qPCR Reaction Buffer (2G) (Part No. NNB004) 400 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy Primer \u0026amp; Probe MIX (2G) (Part No. NNC065) 75 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eUser Guide \/ Instructions for use\u003c\/span\u003e\u003cstrong\u003eRoom temperature (follow handling instructions)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For research use only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean area: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\n \u003cp\u003e• Thaw kit components at 2–8°C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n \u003cp\u003e• Protect My qPCR Reaction Buffer from light during storage and use.\u003c\/p\u003e\n \u003cp\u003e• If Internal Control (IC) was added during extraction, substitute equal volume of DNA Dilution Buffer in qPCR mix instead of IC.\u003c\/p\u003e\n \u003cp\u003e• Follow recommended plate layout and include controls (PC, NTC, NCS) in duplicate.\u003c\/p\u003e\n \u003cp\u003e• If VIC (IC) signal is inhibited (Ct ≥ threshold), repeat extraction or remove inhibitors and repeat assay.\u003c\/p\u003e\n \u003cp\u003e• Dispose of biological and chemical waste according to local regulations.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated with a limit of detection of 10 CFU\/mL.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the Internal Control (IC) be added before extraction?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. IC (2G) may be added to the sample prior to extraction to evaluate extraction efficiency; if IC is added during extraction, omit IC when preparing the qPCR mix and add equal volume of DNA Dilution Buffer instead.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which qPCR instruments are compatible with this kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500 and Roche LightCycler 480. Use instrument software to set FAM for target and VIC for IC.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted (Ct thresholds)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eAcceptance criteria: Positive control (PC) FAM Ct \u0026lt; 35; NTC FAM Ct ≥ 40 or no amplification; Unknown sample: FAM Ct \u0026lt; 40 (at least one well) with normal amplification = Positive. If IC (VIC) is inhibited (VIC Ct ≥ threshold or absent), repeat extraction or remove inhibitors and retest.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45191337345205,"sku":"1509841","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Detection_Kit_2G.jpg?v=1742362689"},{"product_id":"mycobacterium-phlei-sensitivity-standard-10-cfu-mycoshentek™","title":"Mycobacterium Phlei Sensitivity Standard (10 CFU) MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strain); -20 °C (DDB) (stable for 12 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycobacterium Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT) for detection of mycobacteria. Available as defined loads (10 CFU or 100 CFU per tube), the standards are not intended for culture and are used by adding a specified volume of sample matrix to each tube to prepare test solutions for downstream DNA extraction and real-time PCR (qPCR) analysis. These standards support method validation and routine QC for mycobacterial DNA extraction and qPCR assays and are aligned with recommendations from the European Pharmacopoeia (EP), United States Pharmacopoeia (USP) and Chinese Pharmacopoeia (ChP). The product is supplied with a DNA Dilution Buffer (DDB) for preparation of test solutions. Appropriate sample DNA extraction prior to PCR is recommended to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal load per tube\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU or 100 CFU per tube (provided as separate catalogue items)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct formats \/ pack size\u003c\/td\u003e\r\n \u003ctd\u003eEach format supplied as 3 tubes per pack: 10 CFU × 3 tubes (NNE002) or 100 CFU × 3 tubes (NNE001); DNA Dilution Buffer (NND001) 1.5 mL × 3 tubes\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReference standard for validation and sensitivity assessment of NAT\/qPCR assays for mycobacteria; not for culture, not infectious\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage temperatures\u003c\/td\u003e\r\n \u003ctd\u003eMycobacterium strains: -65 °C; DNA Dilution Buffer (DDB): -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eStable for 12 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended preparation volume\u003c\/td\u003e\r\n \u003ctd\u003eFor 10 CFU tubes, supplement sample matrix to a total volume of 400 µL per tube (follow label instructions for other formats)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eDesigned for use with MycoSHENTEK® Mycobacteria DNA Extraction Kit and MycoSHENTEK® Mycobacteria DNA Detection Kit and other validated qPCR workflows\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSafety \/ infectivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNon-infectious material; not intended for culture\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWarnings on dilution\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eEach strain must not be diluted — dilution may cause uneven distribution and risk of undetected contamination\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDefined CFU reference standards:\u003c\/strong\u003e Provided at fixed nominal loads (10 CFU and 100 CFU per tube) for sensitivity assessment and limit-of-detection verification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious, culture-free:\u003c\/strong\u003e Standards are non-infectious and not intended for culture, reducing biosafety concerns while enabling NAT validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-compatible:\u003c\/strong\u003e Optimized for downstream DNA extraction and qPCR-based detection workflows; suitable as positive controls and sensitivity standards.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRegulatory-aligned:\u003c\/strong\u003e Supports validation approaches recommended by EP, USP and ChP to replace lengthy culture-based methods.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConvenient format:\u003c\/strong\u003e Supplied as multiple tubes with matching DNA Dilution Buffer to simplify test solution preparation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of NAT \/ qPCR methods for mycobacteria detection\u003c\/li\u003e\r\n \u003cli\u003eLimit of detection (LOD) and sensitivity verification of qPCR assays\u003c\/li\u003e\r\n \u003cli\u003eRoutine quality control and proficiency testing of mycobacterial molecular assays\u003c\/li\u003e\r\n \u003cli\u003eMethod transfer and regulatory method validation in accordance with EP\/USP\/ChP recommendations\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei Sensitivity Standard (10 CFU) (NNE002) — 10 CFU × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei Sensitivity Standard (100 CFU) (NNE001) — 100 CFU × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-65 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 3 tubes per concentration format (each tube contains the stated CFU); use one tube per control\/test as required | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Each strain must not be diluted; dilution may cause uneven distribution and risk of undetected contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate reagents to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate DNA extraction prior to PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control (matrix negative control) for each test; sample matrix may cause PCR inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• This product is intended for research use only and must not be used for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• For safety, use appropriate laboratory PPE (lab coat, disposable gloves) and follow biosafety cabinet procedures when handling.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503603.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503603.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of MycoSHENTEK Mycobacterium Sensitivity Standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThey are non-infectious reference standards intended to validate NAT\/qPCR methods, assess assay sensitivity and serve as positive controls; they are not for culture or clinical diagnosis.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the standards be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycobacterial strain tubes at -65 °C and the DNA Dilution Buffer (DDB) at -20 °C. The kit shelf life is 12 months; check the expiration date on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I dilute the strain tubes to prepare lower concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. Each strain must not be diluted because dilution may cause uneven distribution of mycobacteria and risk undetected contamination. Instead use the provided nominal formats or validated dilution procedures if supported by your internal validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Do I need to extract DNA before qPCR?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. Appropriate sample DNA extraction is strongly recommended before PCR to reduce inhibition and maximize assay sensitivity. Follow the instructions of your DNA extraction kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"10 CFU\/tube x 3 tubes","offer_id":45186793537717,"sku":"1503603","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacterium_Phlei_Sensitivity_Standard_100_CFU.jpg?v=1742263201"},{"product_id":"mycobacterium-phlei-sensitivity-standard-100-cfu-mycoshentek™","title":"Mycobacterium Phlei Sensitivity Standard (100 CFU) MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-65 °C (strain storage); -20 °C (DNA Dilution Buffer), shelf life 12 months\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eThe MycoSHENTEK® Mycobacterium Sensitivity Standards are non-infectious reference standards designed to validate the robustness and sensitivity of nucleic acid amplification techniques (NAT), specifically qPCR, for detection of Mycobacterium. Available as defined nominal concentrations (10 CFU\/tube and 100 CFU\/tube), the standards are not intended for culture. To prepare test samples, add the specified volume of sample matrix to the standards to reach the desired final concentration (for 10 CFU tubes supplement to a total volume of 400 µL), then perform DNA extraction and real-time PCR. The product is intended as a positive control\/validation standard and is compatible with the MycoSHENTEK® Mycobacteria DNA Extraction Kit and MycoSHENTEK® Mycobacteria DNA Detection Kit. Compliant with recommendations from EP, USP and ChP for replacing culture methods with validated NAT.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNominal concentration (format)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU per tube (NNE002) and 100 CFU per tube (NNE001)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTube count per kit\u003c\/td\u003e\r\n \u003ctd\u003e3 tubes of mycobacterium standard + 3 tubes DNA Dilution Buffer per kit\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNA Dilution Buffer (DDB)\u003c\/td\u003e\r\n \u003ctd\u003e1.5 mL × 3 tubes (NND001)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage temperature\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStrains: -65 °C; DNA Dilution Buffer: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e12 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidation of NAT\/qPCR sensitivity and robustness; positive control standards (non-infectious)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePreparation guidance\u003c\/td\u003e\r\n \u003ctd\u003eThaw at 2-8 °C; add specified sample matrix volume (for 10 CFU tubes supplement to 400 µL), vortex 10 s, centrifuge briefly, extract DNA, perform qPCR\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRestriction\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNot intended for culture; do not dilute strain tubes\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use sensitivity standards:\u003c\/strong\u003e Provided at defined nominal concentrations (10 CFU and 100 CFU) to support assay sensitivity verification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eNon-infectious materials:\u003c\/strong\u003e Standards are non-infectious and supplied for NAT\/qPCR validation without culture handling risk.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeia-aligned:\u003c\/strong\u003e Designed to support validated NAT approaches recommended by EP, USP and ChP as alternatives to lengthy culture methods.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with MycoSHENTEK kits:\u003c\/strong\u003e Validated workflow includes MycoSHENTEK Mycobacteria DNA Extraction Kit and Mycobacteria DNA Detection Kit for full assay validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConvenient format:\u003c\/strong\u003e Supplied in multiple tubes with accompanying DNA dilution buffer for sample preparation and reproducible use.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eValidation of NAT\/qPCR methods for Mycobacterium detection\u003c\/li\u003e\r\n \u003cli\u003eAssay sensitivity verification and limit-of-detection checks\u003c\/li\u003e\r\n \u003cli\u003eMethod qualification and performance verification per EP\/USP\/ChP\u003c\/li\u003e\r\n \u003cli\u003eQuality control and proficiency testing in R\u0026amp;D and QC laboratories\u003c\/li\u003e\r\n \u003cli\u003eOperator training for sample processing, extraction and qPCR workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei (10 CFU) NNE002\u003c\/span\u003e\u003cstrong\u003e-65 °C (store strain tubes frozen)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) NND001 — 1.5 mL × 3 tubes (supplied with 10 CFU kit)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMycobacterium phlei (100 CFU) NNE001\u003c\/span\u003e\u003cstrong\u003e-65 °C (store strain tubes frozen)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) NND001 — 1.5 mL × 3 tubes (supplied with 100 CFU kit)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Not applicable — supplied as positive control standards (3 tubes per concentration) | Method: qPCR (NAT)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Each strain tube must not be diluted; dilution causes uneven distribution and may create undetected contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Equilibrate reagents to room temperature before use and avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate sample DNA extraction before PCR to reduce inhibition and maximize assay sensitivity.\u003c\/p\u003e\r\n \u003cp\u003e• Do not mix reagents from different batches.\u003c\/p\u003e\r\n \u003cp\u003e• Do not use reagents beyond their expiration date; check label expiration.\u003c\/p\u003e\r\n \u003cp\u003e• Operational deviations from the user guide may affect results.\u003c\/p\u003e\r\n \u003cp\u003e• PCR inhibition may be caused by the sample matrix; include a negative control sample using the sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least one negative control for each test run.\u003c\/p\u003e\r\n \u003cp\u003e• Intended for research use only — not for clinical diagnosis or treatment.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate laboratory clothing and disposable gloves for safety.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503604.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503604.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What are the available concentrations of the MycoSHENTEK sensitivity standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are provided as Mycobacterium phlei at nominal concentrations of 10 CFU per tube and 100 CFU per tube, each supplied as 3 tubes per kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the standards be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore mycobacterium strain tubes at -65 °C. Store DNA Dilution Buffer (DDB) at -20 °C. The kit shelf life is 12 months; check the expiration date on the label.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can these standards be used for culture?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. These standards are non-infectious and not intended for culture; they are supplied for NAT\/qPCR validation only.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included when using these standards?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude at least one negative control with the same sample matrix for each test. Also follow DNA extraction and qPCR kit recommendations to monitor inhibition and assay performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical diagnostic or therapeutic use\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 CFU\/tube x 3 tubes","offer_id":45186799599797,"sku":"1503604","price":1236.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycobacterium_phlei_Sensitivity_Standard_100_CFU.jpg?v=1742363473"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-manual-operation-shentek®","title":"Residual Host Cell DNA Sample Preparation Kit (For manual operation) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic-particle based sample preparation kit designed for efficient and reproducible recovery of trace residual host-cell DNA from a wide range of biological products and complex sample matrices across multiple manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended to be used with SHENTEK® host cell DNA quantitation and size analysis kits. The kit supports multiple host cell types (CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA, etc.), and includes reagents for Proteinase K digestion, DNA binding, washing and elution, with magnetic particles for nucleic acid capture.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input (typical)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (procedure described: add 100 µL sample + 10 µL 5M NaCl before digestion)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepare Proteinase K digestion solution in Proteinase K Buffer. For samples with 0–100 mg\/mL protein add 10 µL Proteinase K per 100 µL buffer; for 100–200 mg\/mL protein add 20 µL Proteinase K per 100 µL buffer. Incubate digestion at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA. Note: Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles usage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAdd 10 µL magnetic particles per sample. Vortex particles 5 seconds to fully resuspend before use. Store magnetic particles at 2–8 °C.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAlcohol addition for binding\u003c\/td\u003e\r\n \u003ctd\u003eAfter binding buffer, add 200 µL isopropanol per sample to facilitate binding.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding incubation\u003c\/td\u003e\r\n \u003ctd\u003eVortex tubes vertically at medium speed for 5 minutes to bind nucleic acids to magnetic particles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash once with 700 µL Wash buffer A (ethanol to be added as instructed), then wash with 700 µL Wash buffer B (70% ethanol prepared separately). Spin 10 seconds between steps and use magnetic stand to separate beads.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBead drying\u003c\/td\u003e\r\n \u003ctd\u003eAir-dry magnetic pellet at room temperature for 30 seconds to 3 minutes (environment dependent) to remove residual ethanol; avoid over-drying which hinders resuspension.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 50–100 µL pre-warmed (70 °C) Elution buffer, vortex 5 seconds, incubate at 70 °C for 7 minutes with intermittent vortexing. Centrifuge 1 min, separate beads on magnetic stand and transfer eluate.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical centrifugation\u003c\/td\u003e\r\n \u003ctd\u003eQuick spins of 10 seconds are used throughout to collect liquids from caps\/walls; final centrifuge after elution 1 minute.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReagent storage conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eWash Buffer A: room temperature (add 40 mL anhydrous ethanol before use); Binding solution \/ Elution buffer \/ Dilution buffer \/ Proteinase K Buffer: room temperature (as labeled); Magnetic particles: 2–8 °C; Proteinase K: (no explicit temperature on label in table) - recommended follow label; Glycogen: -20 °C; Yeast tRNA: per label.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under their appropriate conditions for up to 24 months (check label expiration dates).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH requirement\u003c\/td\u003e\r\n \u003ctd\u003eAdjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction if sample pH \u0026lt;5 or \u0026gt;9.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n \u003ctd\u003eUse Negative Control Sample (NCS) processed in parallel and Sample Extraction Recovery Control (ERC) spiked at 2–10× the amount quantified in the unspiked sample to monitor extraction recovery and accuracy.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic-particle based extraction:\u003c\/strong\u003e Magnetic particle separation technology enables efficient capture and reproducible recovery of trace residual DNA from complex biological matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad host-cell compatibility:\u003c\/strong\u003e Validated for multiple host cells including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Compatible with manual preparation or automated extraction using the rHCDpurify instrument.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized for downstream quantitation:\u003c\/strong\u003e Extracted DNA is suitable for SHENTEK® host cell DNA quantitation and size analysis kits and analysis on real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-configured reagents for 100 extractions:\u003c\/strong\u003e Complete reagent set supplied for 100 DNA extractions, with specific buffers and additives (Glycogen, tRNA) for improved recovery of low-abundance DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eResidual host cell DNA extraction and purification from biologics and process intermediates\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for host-cell DNA quantitation by qPCR\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring for biologics and vaccine manufacturing\u003c\/li\u003e\r\n \u003cli\u003eSize analysis and downstream molecular assays of residual DNA\u003c\/li\u003e\r\n \u003cli\u003eExtraction from diverse matrices including upstream and downstream process samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash Buffer A (NND014) 30 mL × 1 bottle (add 40 mL anhydrous ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding Solution (NND016) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution Buffer (NND018) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution Buffer (NND021) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND031) 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label); prepare fresh digestion solution as instructed\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle separation (sample extraction)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, clothing, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash Buffer A before first use; prepare 70% Wash Buffer B separately and label.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and use 100% isopropanol for binding step as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure reagents are clear before use; if cloudy, heat at 37 °C until clear and mix well.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) if outside range to avoid affecting extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid over-drying magnetic pellet; air-dry 30 seconds to 3 minutes depending on environment.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays on the same day after nucleic acid extraction for accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storage below -18 °C may degrade performance.\u003c\/p\u003e\r\n \u003cp\u003e• Use low-retention filter tips and regularly calibrate pipettes to ensure accurate spiking and aspiration.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is recommended per extraction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes using 100 µL of sample per extraction. Adjustments may be made with proportional reagent preparation; follow kit guidelines for dilution and recovery controls.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I use this kit for E. coli and yeast DNA extraction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit supports E. coli and yeast DNA extraction; note that Yeast tRNA should not be added to the Binding buffer when extracting E. coli or yeast DNA.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended incubation conditions for Proteinase K digestion?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution according to sample protein concentration and incubate at 55 °C for 60 minutes as described in the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store the magnetic particles and other reagents?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Glycogen is stored at -20 °C. Other liquid buffers are stored at room temperature as labeled. The kit components can be stored under appropriate conditions for up to 24 months; always check expiration dates on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186936340661,"sku":"SK030203D100","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ResidualHostCellDNASamplePreparationKit_Formanualoperation_-SHENTEK.jpg?v=1742276761"},{"product_id":"chlamydia-spp-universal-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia spp.Universal Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11300 and is now updated to BF-34377554 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11300.pdf\"\u003e\u003cstrong\u003eChlamydia spp.Universal Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11300.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097646261,"sku":"BF-34377554","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d57509db-6b33-4ab9-8fa0-48ac7597d6d9.jpg?v=1774176078"},{"product_id":"chlamydia-muridarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia muridarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia muridarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11340 and is now updated to BF-59544059 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11340.pdf\"\u003e\u003cstrong\u003eChlamydia muridarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11340.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097711797,"sku":"BF-59544059","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_856da674-f3d3-403b-a937-de0fbb07fe22.jpg?v=1774204110"},{"product_id":"chlamydia-caviae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Chlamydia caviae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydia caviae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-11350 and is now updated to BF-43373408 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-11350.pdf\"\u003e\u003cstrong\u003eChlamydia caviae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-11350.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281097810101,"sku":"BF-43373408","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_801f5bde-c74e-4130-bb25-0904a40b2d6e.jpg?v=1774157739"},{"product_id":"aspergillus-campestris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Aspergillus campestris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eAspergillus campestris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26780 and is now updated to BF-47481291 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26780.pdf\"\u003e\u003cstrong\u003eAspergillus campestris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26780.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151287477,"sku":"BF-47481291","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0226ce1c-921c-4fc0-829f-8d02d108d827.jpg?v=1774190208"},{"product_id":"cryptosporidium-galli-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium galli Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium galli Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26811 and is now updated to BF-66087926 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26811.pdf\"\u003e\u003cstrong\u003eCryptosporidium galli Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26811.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281151910069,"sku":"BF-66087926","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0dacbbc6-fad2-40fd-90cd-32262c9bb1a9.jpg?v=1774186735"},{"product_id":"cryptosporidium-hominis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium hominis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26812 and is now updated to BF-76159985 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26812.pdf\"\u003e\u003cstrong\u003eCryptosporidium hominis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26812.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152008373,"sku":"BF-76159985","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_57c29d05-f6fd-4774-9ab4-93002125db01.jpg?v=1774191062"},{"product_id":"cryptosporidium-molnari-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium molnari Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26814 and is now updated to BF-26550785 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26814.pdf\"\u003e\u003cstrong\u003eCryptosporidium molnari Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26814.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152041141,"sku":"BF-26550785","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b81128eb-9cce-4ccd-9f38-df246ccb93fa.jpg?v=1774160359"},{"product_id":"cryptosporidium-meleagridis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium meleagridis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26815 and is now updated to BF-60951899 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26815.pdf\"\u003e\u003cstrong\u003eCryptosporidium meleagridis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26815.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152073909,"sku":"BF-60951899","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4d6e1dfc-516b-4784-bab8-0a2514d20233.jpg?v=1774177384"},{"product_id":"cryptosporidium-muris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium muris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium muris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26816 and is now updated to BF-33047002 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26816.pdf\"\u003e\u003cstrong\u003eCryptosporidium muris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26816.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152106677,"sku":"BF-33047002","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_bfbcfe8a-2f8d-48ee-ae51-212d38b61faf.jpg?v=1774202367"},{"product_id":"cryptosporidium-nasorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium nasorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26817 and is now updated to BF-79876578 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26817.pdf\"\u003e\u003cstrong\u003eCryptosporidium nasorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26817.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152139445,"sku":"BF-79876578","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_c17b4c37-67b8-4a1e-b8af-8877a07ce02c.jpg?v=1774192338"},{"product_id":"cryptosporidium-occultus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium occultus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26818 and is now updated to BF-83455011 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26818.pdf\"\u003e\u003cstrong\u003eCryptosporidium occultus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26818.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152172213,"sku":"BF-83455011","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4e7d04d4-9142-4465-8f00-690b68a2fe2f.jpg?v=1774201908"},{"product_id":"cryptosporidium-ryanae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ryanae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26819 and is now updated to BF-61866087 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26819.pdf\"\u003e\u003cstrong\u003eCryptosporidium ryanae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26819.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152204981,"sku":"BF-61866087","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_8d59fbe0-0b16-4251-ba27-c36dda80c31e.jpg?v=1774206225"},{"product_id":"cryptosporidium-suis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium suis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium suis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26821 and is now updated to BF-19266672 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26821.pdf\"\u003e\u003cstrong\u003eCryptosporidium suis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26821.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152303285,"sku":"BF-19266672","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_d7482826-c68f-4b4e-87e2-a698eb209f6f.jpg?v=1774201916"},{"product_id":"cryptosporidium-scophthalmi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scophthalmi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26822 and is now updated to BF-94766909 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26822.pdf\"\u003e\u003cstrong\u003eCryptosporidium scophthalmi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26822.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152336053,"sku":"BF-94766909","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_61cc6c72-cb76-4a84-8f36-5b1f7a1103db.jpg?v=1774177268"},{"product_id":"cryptosporidium-saurophilum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium saurophilum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26823 and is now updated to BF-46075436 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26823.pdf\"\u003e\u003cstrong\u003eCryptosporidium saurophilum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26823.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152368821,"sku":"BF-46075436","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e47a134c-3ffb-48db-838c-75102c21767c.jpg?v=1774206816"},{"product_id":"cryptosporidium-scrofarum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium scrofarum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26824 and is now updated to BF-91078992 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26824.pdf\"\u003e\u003cstrong\u003eCryptosporidium scrofarum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26824.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152401589,"sku":"BF-91078992","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_ccebb0e2-16d8-4627-96c1-ad2d2c35701d.jpg?v=1774200598"},{"product_id":"cryptosporidium-ubiquitum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium ubiquitum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26825 and is now updated to BF-22799761 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26825.pdf\"\u003e\u003cstrong\u003eCryptosporidium ubiquitum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26825.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152434357,"sku":"BF-22799761","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_59b61532-0e3b-4f22-b1cc-33fe450bbd97.jpg?v=1774191859"},{"product_id":"cryptosporidium-varaii-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium varaii Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26826 and is now updated to BF-20436497 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26826.pdf\"\u003e\u003cstrong\u003eCryptosporidium varaii Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26826.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152467125,"sku":"BF-20436497","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_90a4f4a7-c3e5-4bdb-8a21-742ef231a111.jpg?v=1774191497"},{"product_id":"cryptosporidium-viatorum-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium viatorum Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26827 and is now updated to BF-51393006 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26827.pdf\"\u003e\u003cstrong\u003eCryptosporidium viatorum Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26827.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152696501,"sku":"BF-51393006","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_00593ebd-f248-45a2-9826-dd3e74f20d53.jpg?v=1774205906"},{"product_id":"cryptosporidium-wrairi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium wrairi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26828 and is now updated to BF-42857399 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26828.pdf\"\u003e\u003cstrong\u003eCryptosporidium wrairi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26828.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281152794805,"sku":"BF-42857399","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_aa5a97d3-4f64-4c17-8fc4-bab8947c969b.jpg?v=1774171625"},{"product_id":"cryptosporidium-andersoni-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium andersoni Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26850 and is now updated to BF-56975258 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26850.pdf\"\u003e\u003cstrong\u003eCryptosporidium andersoni Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26850.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153024181,"sku":"BF-56975258","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6cc007a5-0583-4244-997d-9fc1d510e6e2.jpg?v=1774204766"},{"product_id":"cryptosporidium-bovis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium bovis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26860 and is now updated to BF-59618136 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26860.pdf\"\u003e\u003cstrong\u003eCryptosporidium bovis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26860.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153089717,"sku":"BF-59618136","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_769c899e-1b3d-45c4-af3e-7be8b290204e.jpg?v=1774189072"},{"product_id":"cryptosporidium-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26870 and is now updated to BF-53887329 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26870.pdf\"\u003e\u003cstrong\u003eCryptosporidium canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26870.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153188021,"sku":"BF-53887329","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_50324e85-c3cf-44c6-85d4-4aa1c0906d50.jpg?v=1774189063"},{"product_id":"cryptosporidium-felis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium felis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium felis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26880 and is now updated to BF-39439124 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26880.pdf\"\u003e\u003cstrong\u003eCryptosporidium felis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26880.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153220789,"sku":"BF-39439124","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_da2b8561-2924-43d9-8c04-d20f6aa0ac11.jpg?v=1774192285"},{"product_id":"cryptosporidium-fayeri-probe-realtime-rt-pcr-kit-without-internal-control","title":"Cryptosporidium fayeri Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-26890 and is now updated to BF-60900951 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-26890.pdf\"\u003e\u003cstrong\u003eCryptosporidium fayeri Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-26890.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45281153253557,"sku":"BF-60900951","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_930ae5ab-4847-4abc-8cd5-ae507e7a812b.jpg?v=1774193639"},{"product_id":"hepatozoon-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Hepatozoon canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHepatozoon canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58000 and is now updated to BF-87699077 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58000.pdf\"\u003e\u003cstrong\u003eHepatozoon canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58000.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282355445941,"sku":"BF-87699077","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_96b39454-3724-4d36-82f1-fa76dc84ab28.jpg?v=1774204757"},{"product_id":"pichia-pastoris-probe-rt-pcr-kit-without-internal-control","title":"Pichia pastoris Probe RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003ePichia pastoris Probe RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58900 and is now updated to BF-75676982 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58900.pdf\"\u003e\u003cstrong\u003ePichia pastoris Probe RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58900.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357051573,"sku":"BF-75676982","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b17d638d-769d-44aa-bbb8-b1e2239b1410.jpg?v=1774187579"},{"product_id":"skygen-saccharomyces-cerevisiae-probe-realtime-rt-pcr-kit-without-internal-control","title":"Saccharomyces cerevisiae Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSaccharomyces cerevisiae Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58910 and is now updated to BF-72442609 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58910.pdf\"\u003e\u003cstrong\u003eSaccharomyces cerevisiae Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58910.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357739701,"sku":"BF-72442609","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b1bacb46-efd0-4dcf-9656-374d20887039.jpg?v=1774205451"},{"product_id":"skygen-schizosaccharomyces-pombe-probe-realtime-rt-pcr-kit-without-internal-control","title":"Schizosaccharomyces pombe Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSchizosaccharomyces pombe Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-58920 and is now updated to BF-78477582 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-58920.pdf\"\u003e\u003cstrong\u003eSchizosaccharomyces pombe Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-58920.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282357969077,"sku":"BF-78477582","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b8ed6fd4-f912-4280-9561-0d7065b8de48.jpg?v=1774188414"},{"product_id":"skygen-brucella-inopinata-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella inopinata Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella inopinata Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61211 and is now updated to BF-71626667 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61211.pdf\"\u003e\u003cstrong\u003eBrucella inopinata Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61211.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370388149,"sku":"BF-71626667","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_6c77bc7d-7830-4bb1-9c8d-fd73e65464d8.jpg?v=1774164498"},{"product_id":"skygen-brucella-vulpis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella vulpis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella vulpis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61212 and is now updated to BF-11064734 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61212.pdf\"\u003e\u003cstrong\u003eBrucella vulpis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61212.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370420917,"sku":"BF-11064734","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_20c53d37-2c7f-4234-b07a-831bb1a5bf11.jpg?v=1774187674"},{"product_id":"skygen-brucella-papionis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella papionis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella papionis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61213 and is now updated to BF-21408354 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61213.pdf\"\u003e\u003cstrong\u003eBrucella papionis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61213.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370519221,"sku":"BF-21408354","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_521fcf88-9fdb-42f4-b61d-bb3a9a6c20a3.jpg?v=1774187639"},{"product_id":"brucella-melitensis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella melitensis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella melitensis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61260 and is now updated to BF-20115717 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61260.pdf\"\u003e\u003cstrong\u003eBrucella melitensis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61260.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282370912437,"sku":"BF-20115717","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_66ac9a80-e377-42e4-ac15-5b66fe056fa8.jpg?v=1774176980"},{"product_id":"brucella-maris-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella maris Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella maris Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61270 and is now updated to BF-28670061 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61270.pdf\"\u003e\u003cstrong\u003eBrucella maris Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61270.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371109045,"sku":"BF-28670061","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5ce8460e-55f1-4ed1-922b-f4f1d82d7819.jpg?v=1774163972"},{"product_id":"brucella-ceti-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella ceti Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella ceti Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61280 and is now updated to BF-52942801 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61280.pdf\"\u003e\u003cstrong\u003eBrucella ceti Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61280.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371207349,"sku":"BF-52942801","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_593f4d34-6180-48b9-a558-00daaad0cf12.jpg?v=1774194504"},{"product_id":"brucella-pinnipedialis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Brucella pinnipedialis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBrucella pinnipedialis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-61290 and is now updated to BF-71183683 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-61290.pdf\"\u003e\u003cstrong\u003eBrucella pinnipedialis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-61290.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282371272885,"sku":"BF-71183683","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_9e92dc55-4ce7-48bd-836a-3cc43c8e940c.jpg?v=1774193016"},{"product_id":"spirillum-minus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Spirillum minus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSpirillum minus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-62005 and is now updated to BF-14937533 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-62005.pdf\"\u003e\u003cstrong\u003eSpirillum minus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-62005.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282373632181,"sku":"BF-14937533","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_f48abe93-b00b-47ed-8219-0e4813e2e876.jpg?v=1774202142"},{"product_id":"trypanosoma-lewisi-probe-realtime-rt-pcr-kit-without-internal-control","title":"Trypanosoma lewisi Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eTrypanosoma lewisi Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-66011 and is now updated to BF-23536660 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-66011.pdf\"\u003e\u003cstrong\u003eTrypanosoma lewisi Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-66011.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282382545077,"sku":"BF-23536660","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_4c136fa2-f9aa-4d2f-9e49-779db452005d.jpg?v=1774166591"},{"product_id":"nontuberculous-mycobacteria-probe-realtime-rt-pcr-kit-without-internal-control","title":"Nontuberculous mycobacteria Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eNontuberculous mycobacteria Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-68805 and is now updated to BF-74731677 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-68805.pdf\"\u003e\u003cstrong\u003eNontuberculous mycobacteria Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-68805.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282393817269,"sku":"BF-74731677","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_1a2a902e-54c7-49df-a8a9-4f17aafe6302.jpg?v=1774193497"},{"product_id":"toxocara-canis-probe-realtime-rt-pcr-kit-without-internal-control","title":"Toxocara canis Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eToxocara canis Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-75900 and is now updated to BF-23579083 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\n\u003cp\u003eNot sold in the USA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e \u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-75900.pdf\"\u003e\u003cstrong\u003eToxocara canis Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-75900.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282409218229,"sku":"BF-23579083","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b248119d-14bd-45f7-811c-3d2553ac1ca6.jpg?v=1774196480"},{"product_id":"human-coronavirus-probe-realtime-rt-pcr-kit-without-internal-control","title":"Human Coronavirus Probe Realtime RT-PCR Kit","description":"\u003cstyle\u003e\n \n .josh-faq {\n background: #ffffff;\n color: #000000;\n font-family: 'IBM Plex Sans', sans-serif;\n }\n\n .josh-faq,\n .josh-faq * {\n box-sizing: border-box;\n }\n\n .josh-faq__item {\n border-bottom: 1px solid #e5e7eb;\n padding: 0;\n }\n\n .josh-faq__item:first-child {\n border-top: 1px solid #e5e7eb;\n }\n\n .josh-faq__question {\n width: 100%;\n background: none;\n border: none;\n text-align: left;\n padding: 20px 0;\n font-size: 16px;\n font-weight: 700;\n color: #000;\n font-family: 'IBM Plex Sans', sans-serif;\n cursor: pointer;\n display: flex;\n align-items: center;\n justify-content: space-between;\n gap: 16px;\n line-height: 1.4;\n }\n\n .josh-faq__question:hover {\n color: #055da9;\n }\n\n .josh-faq__icon {\n flex-shrink: 0;\n width: 20px;\n height: 20px;\n position: relative;\n transition: transform 0.25s ease;\n }\n\n .josh-faq__icon::before,\n .josh-faq__icon::after {\n content: '';\n position: absolute;\n background: currentColor;\n border-radius: 2px;\n transition: opacity 0.25s ease, transform 0.25s ease;\n }\n\n .josh-faq__icon::before {\n width: 100%;\n height: 2px;\n top: 50%;\n left: 0;\n transform: translateY(-50%);\n }\n\n .josh-faq__icon::after {\n width: 2px;\n height: 100%;\n left: 50%;\n top: 0;\n transform: translateX(-50%);\n }\n\n .josh-faq__item.is-open .josh-faq__icon::after {\n opacity: 0;\n transform: translateX(-50%) rotate(90deg);\n }\n\n .josh-faq__answer {\n display: none;\n padding: 0 0 20px 0;\n font-size: 16px;\n line-height: 1.6;\n color: #333;\n max-width: 860px;\n }\n\n .josh-faq__answer.is-open {\n display: block;\n }\n\n .josh-faq__answer p {\n margin: 0 0 15px;\n }\n\n .josh-faq__answer p:last-child {\n margin-bottom: 0;\n }\n\n .josh-faq__answer ul {\n margin: 8px 0 12px;\n padding-left: 22px;\n }\n\n .josh-faq__answer li {\n margin-bottom: 6px;\n list-style-type: disc;\n }\n\n .josh-faq__answer a {\n color: #055da9;\n text-decoration: none;\n }\n\n .josh-faq__answer a:hover {\n text-decoration: underline;\n }\n\n @media (max-width: 767px) {\n .josh-faq__question {\n font-size: 15px;\n padding: 16px 0;\n }\n\n .josh-faq__answer {\n font-size: 15px;\n }\n }\n\u003c\/style\u003e\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHuman Coronavirus Probe Realtime RT-PCR Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-88100 and is now updated to BF-30480550 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eOne-step RT-PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eCompatible with commonly used RNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"604\" data-start=\"577\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2× One-Step RT-PCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 μL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/µL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n \u003c!-- FAQ List --\u003e\r\n \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is a ROX Dye included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is an Internal Control included with the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Is validation data available for the kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n What is the lead time for all PCR kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Can I customize this kit?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Will your kits work with any PCR system?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you offer discounts for bulk orders of kits?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n \u003cul\u003e\r\n \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits internationally?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Do you ship qPCR kits on ice packs or at room temperature?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003cdiv class=\"josh-faq__item\"\u003e\r\n \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n Are your kits approved for diagnostic or clinical use?\r\n \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n \u003c\/button\u003e\r\n \u003cdiv class=\"josh-faq__answer\"\u003e\r\n \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-88100.pdf\"\u003e\u003cstrong\u003eHuman Coronavirus Probe Realtime RT-PCR Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-88100.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n var items = document.querySelectorAll('.josh-faq__item');\r\n items.forEach(function (item) {\r\n var btn = item.querySelector('.josh-faq__question');\r\n var answer = item.querySelector('.josh-faq__answer');\r\n btn.addEventListener('click', function () {\r\n var isOpen = item.classList.contains('is-open');\r\n \/\/ Close all\r\n items.forEach(function (el) {\r\n el.classList.remove('is-open');\r\n el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n });\r\n \/\/ Toggle clicked\r\n if (!isOpen) {\r\n item.classList.add('is-open');\r\n btn.setAttribute('aria-expanded', 'true');\r\n answer.classList.add('is-open');\r\n }\r\n });\r\n });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282423406773,"sku":"BF-30480550","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_f97eaeed-ee95-4514-a6f5-119ee8078e73.jpg?v=1774154510"},{"product_id":"quickeasy™-cell-direct-rt-qpcr-kit-sybr-green-i","title":"(96-well) QuickEasy™ Cell Direct RT-qPCR Kit–SYBR Green I","description":"\u003ch2\u003eDescprition\u003c\/h2\u003e\n\u003cp\u003eThis product uses a unique lysis buffer system to rapidly release RNA from cultured cell samples for RT-qPCR reactions, eliminating the time-consuming and laborious RNA purification, and only takes 7 minutes to obtain the required RNA template. Used with the 5× Direct RT Mix and 2× Direct qPCR Mix-SYBR provided in the kit, effective real-time quantitative PCR results can be quickly obtained.\u003c\/p\u003e\n\u003cp\u003e5× Direct RT Mix and 2× Direct qPCR Mix-SYBR have strong inhibitor tolerance, enabling efficient reverse transcription and specific amplification using the sample lysate as template. The kit contains high-RNA-affinity Foregene Reverse Transcriptase, as well as Hot-Start T-Taq DNA Polymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer. When used with the lysis buffer, they enable rapid and simple sample detection with high sensitivity, strong specificity, and excellent stability.\u003c\/p\u003e\n\u003cp\u003eThe kit is designed for micro-scale lysis of cells cultured in 96-well plates, offering excellent uniformity and consistency. The kit components provide 96 lysis reactions, 96 reverse transcription reactions, and 96×2 qPCR reactions, supporting one-batch processing of an entire 96-well cell plate. Avoid contamination and performance degradation of reagents caused by repeated opening, freeze-thaw cycles.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCatalog Number Transition:\u003c\/b\u003e DRT-02011 is the next-generation upgraded version of the former \u003cb\u003eDRT-01011\u003c\/b\u003e and \u003cb\u003eDRT-01012\u003c\/b\u003e kits. Customers searching for the previous catalog numbers can now refer to \u003cb\u003eDRT-02011\u003c\/b\u003e for the updated formulation and optimized performance.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%; height: 176px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd colspan=\"2\" style=\"width: 25%; height: 22px; text-align: center;\"\u003eCOMPONENT\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eDRT-02011\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 25%; height: 44px; text-align: center;\"\u003eNote\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd colspan=\"2\" style=\"width: 25%; height: 22px; text-align: center;\"\u003e(50 μL Lysis System \/ 20 μL RT Reaction System \/ 20 μL qPCR Reaction System)\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"3\" style=\"height: 22px; text-align: center; width: 25%;\"\u003ePart I\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eBuffer CL\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"height: 22px; text-align: center; width: 25%;\"\u003eCell Lysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eForegene Protease Plus II\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eBuffer ST\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e500 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd rowspan=\"5\" style=\"text-align: center; width: 25%;\"\u003ePart II\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eDNA Eraser\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e5× Direct RT Mix\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003eRT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e2× Direct qPCR Mix-SYBR\u003c\/td\u003e\n\u003ctd style=\"width: 25%; height: 22px; text-align: center;\"\u003e1 mL × 2\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"height: 22px; text-align: center; width: 25%;\"\u003eqPCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e50× ROX Reference Dye\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eRNase-Free ddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1.7 mL\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"2\" style=\"text-align: center; width: 25%;\"\u003eInstruction Manual\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1 piece\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eNote: \u003c\/strong\u003eThe DNA Eraser for lysis is packed in Part II.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer CL: Provides optimal conditions for cell lysis reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eForegene Protease Plus II: Lyses cells and releases nucleic acids in the lysis buffer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDNA Eraser: DNA remover, to eliminate the impact of genomic DNA on subsequent experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer ST: Terminates the active substance in lysate to avoid effects on subsequent RT.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e5× Direct RT Mix: Contains high-RNA-affinity Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, stabilizers, enhancers, optimizers, and optimized reverse transcription primers (Random Primer, Oligo(dT) 18 Primer).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e2× Direct qPCR Mix-SYBR: This reagent contains Foreasy HS Taq DNA Polymerase, dNTPs, MgCl₂, reaction buffer, SYBR Green I, PCR optimizer and stabilizer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e50× ROX Reference Dye: Generally used on real-time quantitative PCR instruments of ABI, Stratagene and other companies, it is used to adjust the difference between PCR tubes caused by PCR pipetting errors. The 50× ROX Reference Dye concentration required for different instruments is different, and the user can add it according to the recommended concentration of the instrument.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRNase-Free ddH₂O: RNase-Free sterilized ultrapure water for two-step RT-qPCR reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003ePrecautions: (Be sure to read the precautions carefully before using the kit)\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePay attention to the operation of the experiment to avoid cross-contamination between samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eKeep the experimental environment and utensils clean to avoid RNase contamination and RNA degradation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePlease prepare the cell lysis system fresh and use it immediately.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid repeated freeze-thaw cycles for 2× Direct qPCR Mix-SYBR, as this may reduce PCR efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor research use only.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTransport conditions: Transport on ice packs at \u0026lt;4°C\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStorage conditions: Store Part I at 2-8°C and Part II at -20±5°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/96-well_QuickEasy_Cell_Direct_RT-qPCR_Kit-SYBR_Green_I.pdf?v=1779258690\"\u003e\u003cstrong\u003eInstruction Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n \u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003equickeasy\u003c\/div\u003e","brand":"Foregene","offers":[{"title":"96T","offer_id":45283651748021,"sku":"DRT-02011","price":1450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/96-well_QuickEasy_CellDirectRT-qPCRKit_SYBRGreenIDRT-0201101.jpg?v=1779341309"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/qpcr_Kits.png?v=1757009030","url":"https:\/\/biofargo.com\/collections\/pathogen-detection-kits.oembed?page=40","provider":"Biofargo","version":"1.0","type":"link"}