{"title":"Other ELISA Kits","description":"","products":[{"product_id":"general-advanced-glycation-end-product-age-elisa-kit","title":"General Advanced Glycation End Product (AGE) ELISA Kit","description":"\u003cp\u003eSynonyms: AGEs; Advanced Glycation End Products\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eDetection range: 98.8-8,000ng\/mL\u003c\/p\u003e\n\u003cp\u003eSize: 96T\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAssay length 1-4.5Hours\u003c\/p\u003e\n\u003cp\u003ePrice: inquiry\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eQuality guarantee period: for 12 months\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntroduction\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTest Result\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDescription\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis immunoassay kit allows for the specific measurement of this index in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eOther biological fluids..\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eConform\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIdentification\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eColorimetric\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePositive\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePre-coated, ready to use 96-well strip plate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard (freeze dried)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard Diluent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 20ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDetection Reagent A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1× 120μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDetection Reagent B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1× 120μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAssay Diluent A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 12ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eComposition\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAssay Diluent B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 12ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eConform\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTMB Substhumane\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 9ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 ×6ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWash Buffer(30 x concenthumane)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 ×20ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePlate sealer for 96 wells\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eInstruction manual\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eTest principle\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\nThe microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRecovery\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMatrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eMatrix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecovery range (%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAverage(%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eserum(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e81-93\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e86\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eEDTA plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-97\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e88\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eheparin plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e90-101\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e95\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eLinearity\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\nThe linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：8\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：16\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eserum(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e82-96%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e83-98%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e81-99%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e93-101%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eEDTA plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e88-101%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e86-95%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e90-102%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-93%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eheparin plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-91%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e82-90%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e95-104%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e79-95%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003ePrecision\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.\u003c\/p\u003e\n\u003cp\u003eCV(%) = SD\/meanX100\u003c\/p\u003e\n\u003cp\u003eIntra-Assay: CV\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003eInter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStability\u003c\/b\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\nThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAssay procedure summary\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e1.Prepare all reagents, samples andstandards;\u003c\/p\u003e\n\u003cp\u003e2.Add 100µL standard or sample to each well. Incubate 2 hours at37oC;\u003c\/p\u003e\n\u003cp\u003e3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at37oC;\u003c\/p\u003e\n\u003cp\u003e4.Aspirate and wash 3times;\u003c\/p\u003e\n\u003cp\u003e5.Add 100µL prepared Detection Reagent B. Incubate 1hour at37oC;\u003c\/p\u003e\n\u003cp\u003e6.Aspirate and wash 5times;\u003c\/p\u003e\n\u003cp\u003e7.Add 90µL Substrate Solution. Incubate 15-25 minutes at37oC;\u003c\/p\u003e\n\u003cp\u003e8.Add 50µL Stop Solution. Read at 450nm\u003c\/p\u003e\n \u003cbr\u003e \n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"wytsci","offers":[{"title":"96T","offer_id":52286468587701,"sku":"YB-AGE-Ge","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_30f7416d-9dcb-4a09-8d5b-d1eb2b598893.jpg?v=1753176722"},{"product_id":"e-coli-protein-expression-strains-hcp-elisa-kit-shentek®","title":"E.coli (Protein Expression Strains) HCP ELISA Kit  SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit; opened strips 2-8 °C up to 60 days; reconstituted standard 2-8 °C up to 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e96-well ELISA kit for quantitative measurement of residual host cell proteins (HCPs) from E.coli protein expression strains (originating from BL21). The kit uses a double-antibody sandwich format with an affinity-purified sheep polyclonal capture antibody coated on the microtiter strips and a biotinylated sheep polyclonal detection antibody. Detection is achieved via streptavidin-HRP and TMB substrate with absorbance read at 450 nm (reference 620–650 nm). The kit is intended for research use to monitor HCPs in products expressed in E.coli BL21 (e.g., ILs, rhIFN, rhGM-CSF, rhTNF, EGF\/FGF\/PDGF). No special sample pre-treatment is required other than clarification and suitable dilution; method validation is recommended for each sample matrix.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eDouble-antibody sandwich ELISA (capture antibody coated microtiter strips; biotinylated detection antibody + streptavidin-HRP)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 - 243 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration curve model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL). Typical 4-PL parameters: A = 2.38642; B = -1.02805; C = 85.80515; D = 0.00132; R² = 1.00000\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration points (conc. ng\/mL)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 243; ST2 81; ST3 27; ST4 9; ST5 3; ST6 1; NCS 0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd\u003eNo cross-reactivity with CHO, Vero, HEK293T, and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5 - 8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product derived from E.coli BL21 expression\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStability note\u003c\/td\u003e\r\n                        \u003ctd\u003eDo not mix reagents from different lots. Reconstituted calibration standard and diluted conjugates\/streptavidin-HRP are recommended for single use due to stability.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity capture\/detection:\u003c\/strong\u003e Sheep polyclonal anti-E.coli HCP antibodies used for capture and biotinylated detection maximize recognition of diverse HCP species from BL21 expression strains.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive quantitative range:\u003c\/strong\u003e Measured linear range 1–243 ng\/mL with LLOQ 1.5 ng\/mL using a 4-PL calibration curve.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete ready-to-use kit:\u003c\/strong\u003e Includes coated microtiter strips, lyophilized calibration standard, diluent, wash buffer concentrate, biotinylated conjugate, streptavidin-HRP, TMB substrate, stop solution and sealing films.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStandard ELISA workflow:\u003c\/strong\u003e Uses streptavidin-HRP\/TMB detection compatible with standard plate readers (450 nm with 620–650 nm reference).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for BL21 expression strains:\u003c\/strong\u003e Optimized and validated for residual HCP detection in products expressed in E.coli BL21; method validation required for other strains.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual host cell proteins (HCPs) in products expressed in E.coli BL21\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring (fermentation harvest, clarified bulk)\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of drug substance and drug product for residual E.coli HCPs\u003c\/li\u003e\r\n                    \u003cli\u003eAssay support for bioprocess development and purification optimization for E.coli-expressed proteins (e.g., ILs, rhIFN, rhGM-CSF, rhTNF, EGF\/FGF\/PDGF)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli HCP-E Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003eStore reconstituted standard at 2-8 °C (reconstituted stability: up to 30 days).\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-E Microtiter Strips (pre-coated, 8-well ×12 strips)\u003c\/span\u003e\u003cstrong\u003eStore sealed in bag with desiccant at 2-8 °C (opened strips stable up to 60 days).\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (for calibration standard)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (25 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (2×25 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C. Dilute 10× with ultrapure water before use.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-E Biotinylated Conjugate (100×)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C. Centrifuge before use; dilute freshly 1:100 with 1×Wash Buffer and use immediately.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C. Centrifuge before use; dilute freshly 1:100 with 1×Wash Buffer and use immediately.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (12 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C protected from light; equilibrate to room temperature before use.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl, 6 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; handle with care (corrosive).\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (3 pieces)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C.\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO) — not for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultrapure water; water temperature should not exceed 37 °C during reagent preparation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) which will inhibit HRP and underestimate HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard, diluted biotinylated antibody and diluted streptavidin-HRP are recommended for single use; prepare fresh for each experiment.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles for samples; long-term storage recommended at ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid contact with eyes\/skin; use appropriate PPE and goggles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent well drying and liquid evaporation; seal plate during incubations when indicated.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge concentrated conjugates before use to remove particulates; dilute as instructed.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after adding stop solution.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301301.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301301.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How do I reconstitute the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd exactly 500 µL of the provided Reconstitution Solution to the lyophilized E.coli HCP-E Calibration Standard, invert gently 3–5 times, let stand ~5 minutes until clear, then proceed to prepare serial dilutions as described (ST1–ST6).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should opened components be stored and how long are they stable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the kit and components at 2–8 °C. Opened microtiter strips (in bag with desiccant) are stable up to 60 days; reconstituted calibration standard is stable at 2–8 °C for up to 30 days. Prepare diluted conjugates and streptavidin-HRP fresh for each assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What sample types are compatible and what sample pH is acceptable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCompatible sample types include in-process samples, harvested bulk, drug substance and drug product from E.coli BL21 expression. Sample pH should be between 6.5 and 8.5; otherwise assay results may be abnormal.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What causes high background and how can I troubleshoot it?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eHigh background can be caused by reagent or equipment contamination, insufficient washing, or environment contamination. Use fresh reagents, clean equipment with 75% ethanol, increase wash volume\/times, and ensure thorough removal of wash buffer before next steps.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45187307176117,"sku":"1301301","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E.coli_ProteinExpressionStrains_HCPELISAKit.jpg?v=1742269659"},{"product_id":"e-coli-protein-expression-strains-hcp-elisa-kit-one-step-elisa-shentek®","title":"E.coli (Protein Expression Strains) HCP ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 3 hours (primary incubation) + 15 minutes substrate incubation\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit); Anti-E.coli HCP-E Microtiter Strips 2-8 °C (stable for 60 days); Reconstituted Calibration Standard 2-8 °C (stable for 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is a one-step, solid-phase, double-antibody sandwich Enzyme-Linked Immunosorbent Assay (ELISA) intended to detect residual host cell proteins (HCPs) from E.coli protein expression strains (originated from BL21) in bioprocess samples. Polyclonal capture antibody (sheep anti-E.coli HCP affinity antibody) is pre-coated on microtiter strips. Calibration standards and samples are simultaneously incubated with HRP-conjugated anti-E.coli HCP detection antibody (one-step format). HRP catalyzes TMB oxidation to produce a colorimetric signal measured at 450 nm (with long-wavelength correction 620–650 nm). Concentrations are calculated by a 4-parameter logistic (4PL) fit to the calibration curve. The kit is supplied for research use only and is not intended for clinical diagnostic use.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat \/ Throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit; 8-well × 12 strips\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA with HRP\/TMB colorimetric detection (read at 450 nm, reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1.5–243 ng\/mL, R2 \u0026gt; 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration curve model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL); example parameters A=2.41626, B=1.09106, C=89.16833, D=0.01118, R2=0.99995 (example data)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, Vero, HEK293T, and Hansenula polymorpha HCPs (as tested)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 100 µL HRP detection reagent + 100 µL sample\/standard per well; incubate on microplate thermoshaker at 600 rpm for 3 hours at room temperature; wash; add 100 µL TMB and incubate 15 minutes at room temperature in dark; stop and read\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReading\u003c\/td\u003e\r\n                        \u003ctd\u003eAbsorbance measured at 450 nm with correction wavelength set at 620–650 nm; subtract NCS (blank) and use 4PL fit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (measurements may be compromised outside this range)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume for dilutions\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume 5 µL recommended for accurate dilutions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReading window after stop\u003c\/td\u003e\r\n                        \u003ctd\u003eComplete plate reading within 30 minutes after termination\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step ELISA format:\u003c\/strong\u003e Simultaneous addition of calibration standard\/sample and HRP-conjugated detection antibody reduces hands-on time and simplifies workflow.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ of 1.5 ng\/mL with a linear range up to 243 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStrain-targeted specificity:\u003c\/strong\u003e Polyclonal antibodies generated against E.coli protein expression strains (BL21 origin) to improve detection of residual HCPs from these expression hosts.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and supplied in sealed bag with desiccant for convenience and reproducibility.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated lack of cross-reactivity:\u003c\/strong\u003e No cross-reactivity observed with CHO, Vero, HEK293T, or Hansenula polymorpha HCPs (as tested).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Includes calibration standard, HRP-conjugated detection antibody, diluent, wash buffer concentrate, substrate, stop solution and sealing films.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in products expressed in E.coli BL21 protein expression strains\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process samples, harvested bulk, drug substance and drug product testing during downstream processing\u003c\/li\u003e\r\n                    \u003cli\u003eAssay development and validation for bioprocess monitoring of E.coli-expressed biologics (e.g., IL, rhIFN, rhGM-CSF, rhTNF, EGF\/FGF\/PDGF)\u003c\/li\u003e\r\n                    \u003cli\u003eSpike\/recovery and method validation studies for residual HCP measurement (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli HCP‑E Calibration Standard (PNB003), lyophilized (2 bottles)\u003c\/span\u003e\u003cstrong\u003eStore lyophilized at 2-8 °C; reconstitute with 500 µL Reconstitution Solution; reconstituted standard stable 2-8 °C for up to 30 days (single use recommended)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑E.coli HCP‑E Microtiter Strips (PNA003) 8‑well × 12 strips\u003c\/span\u003e\u003cstrong\u003eSealed with desiccant at 2-8 °C; store unused strips in bag with desiccant; stable for up to 60 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) 1 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; use for dilution of calibration standards, HRP (100×) and samples\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; dilute 1:10 with ultra-pure water before use; may crystallize at low temps—redissolve at 37 °C if necessary\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑E.coli HCP‑E:HRP (100×) (PNN001) 1 × 120 µL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; centrifuge before use; dilute 100× in Diluent to prepare 1× prior to assay; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND002) 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; equilibrate to room temperature 20 minutes before use; keep protected from light; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2-8 °C; corrosive—handle with eye\/skin protection\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature; keep clean\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step double-antibody sandwich ELISA (colorimetric HRP\/TMB)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (RUO); not intended for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile disposable tips, tubes and reservoirs; recommended to wipe bench and equipment with 75% ethanol before and after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra‑pure water; water temperature should not exceed 37 °C when preparing wash buffer.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted Calibration Standard and prepared 1× Anti‑E.coli HCP‑E:HRP are recommended for single use due to instability; prepare freshly before each experiment.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate should be colorless; discard and contact supplier if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid contact with eyes, skin and clothing; wear appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it will deactivate HRP and lead to underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended sample pH 6.5–8.5; outside this range results may be compromised.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for dilutions: 5 µL to ensure accuracy.\u003c\/p\u003e\r\n                    \u003cp\u003e• Seal microplate immediately after sample loading to prevent evaporation; avoid drying wells before substrate incubation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301301-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301301-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for detection of HCPs from any E.coli strain?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is specifically designed and validated for E.coli protein expression strains originated from BL21. Use with other E.coli strains requires method validation; the kit is not suitable for E.coli cloning strains or samples processed by alkaline lysis.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare and store the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstitute each lyophilized Calibration Standard bottle with 500 µL Reconstitution Solution, gently mix and let stand for ~5 minutes. Use to prepare serial dilutions as described. Reconstituted standard may be stored at 2–8 °C for up to 30 days but single use is recommended due to potential instability.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay LLOQ is 1.5 ng\/mL with a validated linear range of 1.5–243 ng\/mL (R2 \u0026gt; 0.99 in internal testing).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What sample types are compatible and how should they be prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCompatible sample types include in-process samples, harvested bulk, drug substance and drug product. Samples should be clear and transparent; remove particulates by centrifugation or filtration, avoid repeated freeze-thaw cycles, and adjust pH to within 6.5–8.5. Dilute samples into the calibration range and verify suitability using dilution series and spike recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45187324051637,"sku":"1301301-1","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E.coli_ProteinExpressionStrains_HCPELISAKit_One-stepELISA.jpg?v=1742276232"},{"product_id":"e-coli-k-12-alkaline-lysis-hcp-elisa-kit-shentek®","title":"E.coli (K-12 \u0026 Alkaline Lysis) HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table 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10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store; strips: up to 60 days; reconstituted standard: up to 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA sandwich-format solid-phase ELISA for quantitation of residual host cell proteins (HCPs) derived from E.coli K-12 strains (e.g., DH5α, Top10, JM109) in plasmid DNA (pDNA) intermediates or bulk prepared by alkaline lysis. The kit uses an affinity-purified capture antibody (sheep polyclonal) raised against alkaline-lysis-derived E.coli K-12 HCPs to capture HCPs, a biotinylated detection antibody and streptavidin–HRP for signal generation. Detection is performed with TMB substrate (read at 450 nm after stop), and sample concentrations are calculated against a six-point calibration curve using a 4-parameter logistic (4-PL) regression. The kit is supplied as 96 tests (8 × 12 strips) and is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003e96-well microtiter strips (8 × 12 strips), precoated with sheep anti-E.coli HCP-AC capture antibody\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended sample types\u003c\/td\u003e\r\n                        \u003ctd\u003epDNA intermediates, in-process samples, drug substance and drug product prepared by alkaline lysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 - 729 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL) regression. Example parameters: A = 3.94571; B = -0.57096; C = 1732.85755; D = -0.05957; R² = 0.99973\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, Vero, HEK293T, and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eSample pH should be between 6.5 and 8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical incubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 100 µL, 1 hour at RT with shaking (300–500 rpm) or 2 hours standing; Biotinylated antibody: 45 min at RT; Streptavidin-HRP: 15 min at RT; TMB: 15 min at RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ Reactions\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePart \/ Product No.\u003c\/td\u003e\r\n                        \u003ctd\u003eProduct No.: 1301302; Calibration Standard PNB002; Strips PNA002; other reagents PN* as labeled\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAlkaline-lysis‑targeted antibodies:\u003c\/strong\u003e Sheep polyclonal antibodies raised against HCPs derived from alkaline lysis of E.coli K-12, improving detection accuracy for alkalinely prepared pDNA samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSandwich ELISA sensitivity:\u003c\/strong\u003e Double-antibody sandwich format with biotin–streptavidin–HRP amplification enables a lower limit of quantitation of 3 ng\/mL and a wide dynamic range up to 729 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use precoated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and supplied in sealed bags with desiccant for convenient setup and reduced plate-coating variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit for routine testing:\u003c\/strong\u003e Includes calibration standard (lyophilized), reconstitution solution, wash buffer concentrate, biotinylated detection antibody, streptavidin‑HRP, TMB substrate and stop solution.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated for common cell lines:\u003c\/strong\u003e Demonstrated no cross-reactivity with common non-E.coli host cells (CHO, Vero, HEK293T, Hansenula polymorpha).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual E.coli K-12 host cell proteins in plasmid DNA (pDNA) intermediates produced by alkaline lysis\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of HCP clearance during pDNA purification\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product pDNA for residual E.coli HCPs (research use)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation support and spike-recovery experiments for pDNA analytics\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE.coli HCP-AC Calibration Standard (PNB002) — lyophilized (3 bottles)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; reconstituted standard: 2-8 °C up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-AC Microtiter Strips (PNA002) — 8 well × 12 strips\u003c\/span\u003e\u003cstrong\u003eStore sealed in bag with desiccant at 2-8 °C; opened strips: up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) — 2 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) — 3 × 25 mL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; if crystalline, warm to 37 °C to dissolve before dilution\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-E.coli HCP-AC Biotinylated Conjugate (20×) (PNG002) — 1 × 600 μL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge briefly before use; prepare fresh 1× dilution before assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002) — 1 × 140 μL\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge briefly before use; prepare fresh 1× dilution before assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND002) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003eKeep sealed, protected from light, store at 2-8 °C; equilibrate to RT 20 min before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) — 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; corrosive — handle with appropriate PPE\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 3 pieces\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for clinical or diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Intended for qualified technicians. Users should validate the assay for their sample matrix prior to routine use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples must be clear and free of insoluble material; centrifuge or filter before testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5; samples outside this range may give abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; for long-term storage, keep samples at −70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use ultra-pure water and ensure water temperature does not exceed 37 °C when preparing reagents.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wash wells thoroughly and avoid drying wells prior to substrate addition.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB must be colorless before use; read plate within 30 minutes after adding stop solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is 1 M HCl — avoid contact with skin and eyes; use appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inhibits HRP and will underestimate HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare biotinylated antibody and streptavidin-HRP dilutions fresh for each assay; recommended single-use for some prepared solutions.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301302.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301302.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is optimized for pDNA samples and intermediates prepared by alkaline lysis from E.coli K-12 strains (e.g., DH5α, Top10, JM109). Samples should be clear and within pH 6.5–8.5.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe lower limit of quantitation (LLOQ) is 3 ng\/mL and the assay is linear from 3 to 729 ng\/mL (R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I incubate the plate without a thermoshaker?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — if a microplate thermoshaker is not available, incubate samples standing for 2 hours at room temperature instead of 1 hour with shaking.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I handle reagents after opening?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore opened components at 2–8 °C as specified (e.g., strips in bag with desiccant up to 60 days; reconstituted standard up to 30 days). Prepare certain dilutions fresh and use single-use when recommended.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Not for clinical diagnostic use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45187364716725,"sku":"1301302","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E.coli_K-12_Alkaline_Lysis_HCP_ELISA_Kit.jpg?v=1742278564"},{"product_id":"mdck-hcp-elisa-kit-shentek®","title":"MDCK HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (unopened; opened components: strips 60 days, reconstituted standard 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis SHENTEK MDCK HCP ELISA Kit is a one-step, solid-phase, double-antibody sandwich ELISA for quantitative detection of residual host cell proteins (HCPs) from MDCK (Madin–Darby canine kidney) cells. A sheep polyclonal anti-MDCK HCP affinity-purified capture antibody is coated on microtiter strips and an HRP-conjugated sheep anti-MDCK HCP detection antibody (used simultaneously with standards\/samples) enables colorimetric detection with TMB\/H2O2 substrate. After incubation and washing, HRP catalyzes TMB oxidation (blue, max absorbance ~655 nm) and reaction is stopped to give a yellow product read at 450 nm (with 620–650 nm reference). Sample HCP concentrations are interpolated from a 4‑parameter logistic (4‑PL) calibration curve constructed from supplied calibration standards.\u003c\/p\u003e\n\u003cp\u003eDesigned for research use only (not for clinical use). Supplied as a 96-test kit with pre-coated microtiter strips, lyophilized calibration standard, HRP-conjugated detection antibody (100×), buffers and substrates. Typical application includes in-process testing and final product testing for MDCK-derived biologics including vaccines and influenza virus products.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit format \/ throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit (8 wells × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay principle\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA, colorimetric detection (TMB, read at 450 nm with 620–650 nm reference)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2–200 ng\/mL (4‑PL curve), R2 ≥ 0.990 (typical R2 reported 0.9994)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration\u003c\/td\u003e\r\n                        \u003ctd\u003eEight-point calibration curve (ST1–ST8) with 4‑parameter logistic (4‑PL) regression; example 4‑PL parameters: A=9.57058, B=-1.04748, C=399.32097, D=0.00304, R2=0.9994\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical OD data (reference)\u003c\/td\u003e\r\n                        \u003ctd\u003eExample OD450–620nm averages: 200 ng\/mL = 3.237; 100 ng\/mL = 1.931; 50 ng\/mL = 1.089; 25 ng\/mL = 0.610; 10 ng\/mL = 0.312; 5 ng\/mL = 0.210; 2 ng\/mL = 0.148; 1 ng\/mL = 0.133; 0 ng\/mL = 0.111\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with E. coli, Pichia pastoris, or Sf9 cell HCPs\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product from MDCK-based processes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (outside this range may cause abnormal results)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions (typical)\u003c\/td\u003e\r\n                        \u003ctd\u003eSimultaneous addition of 1×Anti-MDCK:HRP and samples\/standards; incubate on microplate thermoshaker at 600 rpm for 3 hours at room temperature; wash ×5; add TMB and incubate 15 minutes at RT; add stop solution and read\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReadout\u003c\/td\u003e\r\n                        \u003ctd\u003eAbsorbance at 450 nm with reference at 620–650 nm; OD450–reference used for curve fitting\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReagent stability (opened)\u003c\/td\u003e\r\n                        \u003ctd\u003eAnti‑MDCK HCP microtiter strips: store in bag with desiccant at 2–8 °C up to 60 days; reconstituted calibration standard: 2–8 °C up to 30 days\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step simultaneous detection:\u003c\/strong\u003e Detection antibody (HRP-conjugated) is added simultaneously with standards\/samples to simplify workflow and reduce steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 2 ng\/mL with a linear dynamic range up to 200 ng\/mL using 4‑PL fitting.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMDCK-specific antibody coverage:\u003c\/strong\u003e Sheep polyclonal affinity-purified antibodies raised against MDCK HCPs provide broad coverage of host cell proteins from MDCK cells.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with capture antibody in sealed bag with desiccant for ease of use and storage.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMinimal cross-reactivity:\u003c\/strong\u003e Demonstrated no cross-reactivity with common non-MDCK host cells (E. coli, P. pastoris, Sf9).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual host cell proteins (HCPs) in products manufactured with MDCK host cells\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing of vaccines and influenza virus products produced in MDCK cells\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring (harvested bulk, intermediates)\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of drug substance and drug product for MDCK-derived HCP contamination\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMDCK HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003eLyophilized; reconstitute with 500 μL Reconstitution Solution; reconstituted standard store 2–8 °C up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑MDCK HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips in sealed bag with desiccant; store at 2–8 °C; after opening store in bag with desiccant at 2–8 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e2 × 1.5 mL; room temperature equilibration before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultrapure water before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti‑MDCK:HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 μL HRP‑conjugated affinity‑purified sheep antibody; dilute 100× in Diluent before use; centrifuge before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, protect from light, equilibrate to RT before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; corrosive—avoid contact with skin\/eyes; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces; room temperature storage\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (One-step double-antibody sandwich, HRP\/TMB colorimetric)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit to room temperature for 20 minutes before use; return reagents to 2–8 °C after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh 1×Wash Buffer and 1×Anti‑MDCK:HRP prepared immediately before use; prepare Anti‑MDCK:HRP by diluting 100× concentrate in Diluent and use immediately.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use Wash Buffer Concentrate or Diluent if cloudy or contains precipitates; heat to 37 °C to dissolve if necessary.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles of samples; for long-term storage use ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples should be clear\/transparent; remove particulates by centrifugation or filtration prior to assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5 to avoid abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive. Avoid contact with skin, eyes, and clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid sodium azide (NaN3) in samples or buffers as it will inactivate HRP and lead to underestimated HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 30 minutes after stopping reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended minimum pipetting volume for accurate dilutions: 5 μL.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301308.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301308.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for clinical diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This kit is for Research Use Only (RUO) and is not intended for clinical diagnostic use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I reconstitute the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 500 μL of the provided Reconstitution Solution to the lyophilized MDCK HCP Calibration Standard, gently invert 3–5 times, let stand 5 minutes until clear. Use the reconstituted standard to prepare the ST1–ST8 dilution series as described in the manual.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay LLOQ is 2 ng\/mL and the validated dynamic range is 2–200 ng\/mL using a 4‑parameter logistic (4‑PL) calibration curve.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What precautions should I take for samples and reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eEnsure samples are free of particulates (centrifuge\/filtrate), maintain pH 6.5–8.5, avoid sodium azide, minimize freeze-thaw cycles, prepare 1× reagents fresh, and follow recommended incubation and washing steps carefully.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45188968775861,"sku":"1301308","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MDCK_HCP_ELISA_Kit.jpg?v=1742282635"},{"product_id":"vero-hcp-elisa-kit-shentek®","title":"Vero HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (refer to label)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThe SHENTEK Vero HCP ELISA Kit is a one-step, solid-phase double-antibody sandwich ELISA for quantitation of host cell proteins (HCPs) derived from Vero cells in bioproducts (suitable for in-process samples, harvested bulk, drug substance and drug product produced by sonication cell lysis). The assay uses an affinity-purified sheep polyclonal capture antibody coated on microtiter strips and an HRP-conjugated sheep anti-Vero HCP detection antibody added simultaneously with standards or samples. HRP catalyzes TMB oxidation producing a colorimetric signal (read at 450 nm with correction 620–650 nm). HCP concentration is calculated from a 4-parameter logistic (4PL) calibration curve generated from supplied calibration standards.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of Vero cell-derived host cell proteins (HCPs) in bioproducts; Research Use Only (RUO)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size \/ total reactions\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3–243 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards (nominal concentrations)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 243 ng\/mL; ST2 81 ng\/mL; ST3 27 ng\/mL; ST4 9 ng\/mL; ST5 3 ng\/mL; ST6 1 ng\/mL; NCS 0 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity reported with E. coli, P. pastoris and Sf9 cells\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with correction 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation times\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/antibody incubation: 3 hours at room temperature (600 rpm recommended); TMB substrate incubation: 10 minutes at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash conditions\u003c\/td\u003e\r\n                        \u003ctd\u003e1× Wash Buffer, 340 µL per well, 5 washes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWorking volumes\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL 1×Anti-Vero:HRP per well + 100 µL sample or standard per well; TMB 100 µL\/well; Stop solution 50 µL\/well\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStop solution\u003c\/td\u003e\r\n                        \u003ctd\u003e1 M HCl (corrosive)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage (kit \u0026amp; components)\u003c\/td\u003e\r\n                        \u003ctd\u003eKit: 2–8 °C; Microtiter strips stored sealed with desiccant at 2–8 °C (up to 30 days after opening); Reconstituted Calibration Standard: -20 °C, freeze\/thaw ≤3 cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume recommended for accurate pipetting: 5 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eResult calculation\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4PL) fit of calibration curve; subtract NCS, use dilution factor to get sample concentration\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step assay design:\u003c\/strong\u003e Simultaneous addition of HRP-conjugated detection antibody with standards\/samples simplifies workflow and reduces hands-on steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Lower limit of quantitation (LLOQ) of 3 ng\/mL and linear range up to 243 ng\/mL (R² ≥ 0.990).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh antibody coverage (sheep polyclonal):\u003c\/strong\u003e Affinity-purified sheep polyclonal antibodies provide broad coverage of Vero-derived host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with capture antibody and packaged with desiccant for convenient setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit components:\u003c\/strong\u003e Includes calibration standards, diluent, wash buffer concentrate, HRP-conjugated detection antibody, TMB substrate and stop solution for a full assay.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e Manufacturer reports no cross-reactivity with common alternative host systems (E. coli, P. pastoris, Sf9).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual Vero cell host cell proteins (HCPs) in in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eAnalysis of harvested bulk material\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product for Vero HCP content\u003c\/li\u003e\r\n                    \u003cli\u003eAssay development and validation in bioprocess monitoring and purification workflow optimization (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero HCP Calibration Standard (PNB012) — lyophilized\u003c\/span\u003e\u003cstrong\u003eStore lyophilized; reconstitute with 500 µL Reconstitution Solution; reconstituted standard stored at -20 °C (freeze\/thaw ≤3 cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Vero HCP Microtiter Strips (PNA013) — 8-well × 12 strips\u003c\/span\u003e\u003cstrong\u003ePre-coated strips stored in vacuum bag with desiccant at 2–8 °C; after opening store sealed with desiccant at 2–8 °C (up to 30 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002) — 1 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C (used only to dissolve calibration standard)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C (for dilution of standards, Anti-Vero:HRP and samples)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; dilute 10× with ultra-pure water before use; warm to 37 °C if crystallized\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Vero:HRP (100×) (PNN007) — 1 × 120 µL\u003c\/span\u003e\u003cstrong\u003e2–8 °C; centrifuge before use; dilute 100× in Diluent to prepare 1× working solution; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND005) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2–8 °C, sealed and protected from light; equilibrate to RT before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) — 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; handle as corrosive (avoid contact with eyes\/skin\/clothing)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature; use to cover plate during incubation to prevent evaporation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (one-step double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C; check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) — it will inactivate HRP and cause underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standards and Anti-Vero:HRP working solution are recommended for single use due to instability; prepare fresh before each experiment.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB substrate should be colorless before use; discard and contact provider if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is 1 M HCl — corrosive; avoid contact with eyes, skin, and clothing and follow appropriate safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5; values outside this range may cause abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles; for long-term storage of samples, -70 °C recommended.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after termination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use ultra-pure or sterile water for dilutions and ensure temperature does not exceed 37 °C when preparing solutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for accurate dilution: 5 µL.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301309.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301309.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Is this kit suitable for clinical diagnostic use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. This product is intended for Research Use Only (RUO) and is not for clinical or diagnostic use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 3–243 ng\/mL with an LLOQ of 3 ng\/mL and reported R² ≥ 0.990.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit and reconstituted standards be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the complete kit at 2–8 °C. Reconstitute calibration standard per instructions and store reconstituted standard at -20 °C (limit freeze\/thaw cycles to ≤3). Keep unused strips sealed with desiccant at 2–8 °C.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended incubation times and reading parameters?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eIncubate samples with 1× Anti-Vero:HRP for 3 hours at room temperature (600 rpm recommended). After washing, incubate with TMB for 10 minutes at room temperature. Read absorbance at 450 nm with correction wavelength 620–650 nm.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189061083317,"sku":"1301309","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/VeroHCPELISAKit.jpg?v=1742286379"},{"product_id":"sf9-hcp-elisa-kit-shentek®","title":"Sf9 HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 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.shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit; check label for expiration)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eThis is a solid-phase, one-step double-antibody sandwich ELISA kit for quantitative detection of residual host cell proteins (HCPs) from Spodoptera frugiperda (Sf9) insect cells. A sheep polyclonal anti-Sf9 HCP antibody is coated on microtiter strips to capture HCPs; HRP-conjugated anti-Sf9 antibody is used as detection reagent. Calibration Standards and samples are added together with the HRP-conjugated antibody, followed by incubation, washing, TMB substrate development (HRP catalyzes oxidation of TMB producing color change) and stop solution addition. Absorbance is read at 450 nm with long-wavelength correction (620–650 nm). Concentrations are calculated using a 4-parameter logistic (4-PL) fit to the calibration curve. The kit is intended for research use only and is not for clinical diagnostic use.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (capture antibody coated strips; simultaneous addition of HRP-conjugated detection antibody and samples\/standards)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3–243 ng\/mL; 4-PL fit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL); example curve parameters provided (A, B, C, D) with R2≥0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve performance\u003c\/td\u003e\r\n                        \u003ctd\u003eExample R2 = 1.000 (reference data provided); reference standard concentrations: 0, 1, 3, 9, 27, 81, 243 ng\/mL with corresponding OD values\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with correction wavelength 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample + 1×Anti-Sf9:HRP incubation on thermoshaker: 3 hours at room temperature (600 rpm recommended); TMB substrate incubation: 30 minutes at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity reported with MDCK, Vero, HEK293T, CHO, E. coli and P. pastoris\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH range\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (samples outside this range may cause abnormal results)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash buffer\u003c\/td\u003e\r\n                        \u003ctd\u003eWash Buffer Concentrate (10×) supplied; dilute 1:10 with ultra-pure water to prepare 1×Wash Buffer; recommended 5 washes, 340 µL per well\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControl \u0026amp; replicates\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommend 2–3 replicates per sample and inclusion of NCS (negative control) and spiked recovery controls (SRC) for validation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStability of opened components\u003c\/td\u003e\r\n                        \u003ctd\u003eMicrotiter strips: store in bag with desiccant at 2–8 °C up to 30 days; reconstituted calibration standard: short-term 2–8 °C, long-term aliquot and store below -20 °C (avoid \u0026gt;3 freeze-thaw cycles)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSafety\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStop Solution is 1 M HCl (corrosive) — avoid contact with eyes, skin, clothing; avoid samples containing sodium azide (inactivates HRP)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step format:\u003c\/strong\u003e Simultaneous addition of HRP-labeled detection antibody and standards\/samples reduces hands-on steps and assay time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Low limit of quantification (LLOQ) of 3 ng\/mL with linear range up to 243 ng\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad antibody coverage:\u003c\/strong\u003e Sheep polyclonal anti-Sf9 HCP antibody provides broad recognition of Sf9 host cell proteins; coverage assessed by mainstream methods.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified capture antibody in sealed bags with desiccant for convenience and reduced preparation time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e No cross-reactivity observed with common cell lines and microbial expression hosts tested (MDCK, Vero, HEK293T, CHO, E. coli, P. pastoris).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Contains standards, detection antibody (HRP), diluent, wash buffer concentrate, substrate (TMB), stop solution and sealing film for a full ELISA workflow.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual host cell proteins (HCPs) in products manufactured using Sf9 insect cells\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of recombinant proteins produced in Sf9 cells\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control of vaccines produced using baculovirus\/Sf9 systems\u003c\/li\u003e\r\n                    \u003cli\u003eResidual HCP measurement for recombinant AAV vectors produced in BEVS\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process samples, harvested bulk, drug substance and drug product testing during R\u0026amp;D\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSf9 HCP Calibration Standard (PNB011)\u003c\/span\u003e\u003cstrong\u003eLyophilized; reconstitute with Reconstitution Solution; short-term 2–8 °C, aliquot and store \u0026lt; -20 °C for long-term (avoid \u0026gt;3 freeze-thaw cycles)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Sf9 HCP Microtiter Strips (PNA012)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips; store sealed in bag with desiccant at 2–8 °C; use within 30 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (PNC002)\u003c\/span\u003e\u003cstrong\u003e2 × 1.5 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE004)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C; dilute 1:10 with ultra-pure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Sf9:HRP (100×) (PNN006)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; affinity-purified sheep antibody conjugated to HRP; centrifuge before use; dilute 100× in Diluent immediately before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, keep away from light; equilibrate to RT before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; corrosive — store at 2–8 °C and handle with appropriate PPE\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step sandwich ELISA (solid-phase; HRP\/TMB detection)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2–8 °C. Check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit components to room temperature (≈20 minutes) before use; return to 2–8 °C after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted Sf9 HCP Calibration Standard: short-term 2–8 °C; aliquot and store \u0026lt; -20 °C for long-term; avoid \u0026gt;3 freeze–thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Anti-Sf9:HRP freshly by diluting the 100× stock; use immediately.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless before use; discard if colored. Protect from light during substrate incubation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive; avoid contact with eyes, skin and clothing; use appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it will inactivate HRP and lead to underestimation of HCP levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh sterile\/ultra-pure water (temperature ≤37 °C) for buffer preparation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition and avoid bubbles when pipetting as they affect OD readings.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate the assay and sample dilution strategy before routine use; recommended for qualified technicians only.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use single-use consumables; wipe benches and equipment with 75% ethanol before and after use to reduce contamination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301312.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301312.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 3–243 ng\/mL with an LLOQ of 3 ng\/mL. Calibration is performed using a 4-parameter logistic (4-PL) curve.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the Sf9 HCP Calibration Standard be stored after reconstitution?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstituted standard can be stored short-term at 2–8 °C. For long-term storage, aliquot and store below -20 °C and avoid more than three freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this kit be used for samples from other host cells (e.g., CHO, HEK293)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is developed specifically for Sf9 host cell proteins. The kit was reported to show no cross-reactivity with MDCK, Vero, HEK293T, CHO, E. coli and P. pastoris, so it is not intended for quantifying HCPs from other host cell lines.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What precautions should be taken with sample buffers or additives?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAvoid samples containing sodium azide as it inactivates HRP. Ensure sample pH is between 6.5 and 8.5. If samples are turbid or contain particulates, clarify by centrifugation or filtration and validate sample dilutions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — Not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189111775413,"sku":"1301312","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Sf9_HCP_ELISA_Kit.jpg?v=1742287627"},{"product_id":"p-pastoris-hcp-elisa-kit-one-step-elisa-shentek®","title":"P.pastoris HCP ELISA Kit (One-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e3 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA 96-well solid-phase, double-antibody sandwich one-step ELISA for quantitative detection of residual host cell proteins (HCPs) from Pichia pastoris (P. pastoris) strains (e.g., GS115, X33). The kit uses an affinity-purified sheep polyclonal anti-P.pastoris HCP capture antibody coated on microtiter strips and an HRP-conjugated sheep anti-P.pastoris HCP detection antibody added simultaneously with calibration standard or sample. TMB\/H2O2 is used as the substrate (blue product at ~655 nm; stopped to yellow product with maximum absorbance at 450 nm). Sample concentrations are calculated from a 4-parameter logistic (4-PL) dose–response calibration curve using OD450 (with 620–650 nm reference subtraction). The kit is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (pre-coated microtiter strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate format \/ Kit size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit (8-well × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinear range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2–200 ng\/mL (4-PL fit, R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLLOQ\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e2 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve\u003c\/td\u003e\r\n                        \u003ctd\u003eStandards at 0, 2, 10, 20, 50, 100, 200 ng\/mL; example OD450-620nm average: 0 ng\/mL ~0.078; 2 ng\/mL ~0.114; 10 ng\/mL ~0.270; 20 ng\/mL ~0.434; 50 ng\/mL ~0.761; 100 ng\/mL ~1.233; 200 ng\/mL ~1.704\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eNo cross-reactivity with E. coli, CHO and Sf9 cells; \u0026lt;1% cross-reactivity with Saccharomyces cerevisiae and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003eRead at 450 nm with reference 620–650 nm (OD450 nm - reference)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation times\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/HRP incubation 3 hours at room temperature (600 rpm recommended); TMB substrate incubation 10 minutes; post-stop incubation\/read within recommended time\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample dilution\u003c\/td\u003e\r\n                        \u003ctd\u003eTypically ≥10-fold dilution recommended; method validation recommended to determine optimal dilutions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRead time window\u003c\/td\u003e\r\n                        \u003ctd\u003eComplete reading within 10 minutes after stopping (acceptable up to 30 minutes)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step simultaneous detection:\u003c\/strong\u003e Calibration standard (or sample) and HRP-conjugated detection antibody are added simultaneously to reduce handling steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Low limit of quantitation (LLOQ) of 2 ng\/mL across a 2–200 ng\/mL linear range with 4-PL fitting (R² ≥ 0.990).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity with common non-P.pastoris hosts (E. coli, CHO, Sf9); minimal cross-reactivity (\u0026lt;1%) with closely related yeasts.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use pre-coated strips:\u003c\/strong\u003e Microtiter strips are pre-coated with affinity-purified sheep anti-P.pastoris HCP antibody and packaged with desiccant for convenience.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit:\u003c\/strong\u003e Includes calibration standard, diluent, wash buffer concentrate, HRP conjugate, TMB substrate, stop solution and sealing film for full assay workflow.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell proteins (HCPs) in products manufactured with P. pastoris (e.g., GS115, X33)\u003c\/li\u003e\r\n                    \u003cli\u003eTesting in-process samples, harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eHCP monitoring during downstream purification and process development\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications for bioprocess development and analytical method validation\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eP.pastoris HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003e2 bottles; reconstitute with 500 µL Reconstitution Solution. Reconstituted: 2-8 °C up to 7 days or \u0026lt; -20 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-P.pastoris HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003e8 wells × 12 strips; store in vacuumed bag with desiccant. Opened: 2-8 °C up to 60 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e1 × 1.5 mL; room temperature equilibration for use; used to dissolve calibration standard\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; room temperature; used for dilution of standards, HRP(100×) and samples\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultra-pure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-P.pastoris :HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL HRP-conjugated affinity purified sheep antibody; centrifuge before use; dilute 100× in Diluent immediately prior to use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, keep away from light; equilibrate to room temperature (~20 min) before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; corrosive—avoid contact with eyes, skin and clothing\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C; check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate reagents to room temperature (~20 min) before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare fresh 1× Anti-P.pastoris:HRP by diluting 100× stock in Diluent immediately before use; centrifuge HRP(100×) before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard solutions and anti-P.pastoris HCP antibody solution are recommended for single use due to instability.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inactivates HRP.\u003c\/p\u003e\r\n                    \u003cp\u003e• Sample pH must be between 6.5 and 8.5; adjust or buffer samples accordingly.\u003c\/p\u003e\r\n                    \u003cp\u003e• Inactivate proteolytic enzymes in samples to prevent degradation of HCPs.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate addition; perform recommended wash steps (5×) and remove residual liquid.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — use appropriate PPE and avoid contact with skin\/eyes\/clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 10 minutes after stopping for most stable results (acceptable up to 30 minutes).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile, ultra-pure water; if Wash Buffer or Diluent is cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301313.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301313.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How do I reconstitute the calibration standard?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 500 µL Reconstitution Solution to a lyophilized calibration standard vial, invert gently and let stand ~5 minutes. Combine vials if multiple are used and mix gently. Prepare serial dilutions as described (ST1 = 200 ng\/mL, ST2 = 100 ng\/mL, etc.).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LLOQ) and linear range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLLOQ is 2 ng\/mL. The validated linear range is 2–200 ng\/mL using a 4-parameter logistic (4-PL) fit with R² ≥ 0.990.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What sample dilution should I use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTypically start with at least a 10-fold dilution for in-process, harvest or drug substance samples. Conduct method validation and spike-recovery experiments to determine optimal dilutions for your matrix.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store reconstituted standards and opened components?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eReconstituted standard: 2–8 °C for up to 7 days for short-term use; for long-term storage keep below -20 °C for up to 60 days. Opened microtiter strips: store in bag with desiccant at 2–8 °C up to 60 days.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); Not for clinical diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189143232693,"sku":"1301313","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P.pastoris_HCP_ELISA_Kit_One-step_ELISA.jpg?v=1742290009"},{"product_id":"kanamycin-elisa-kit-shentek®","title":"Kanamycin ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2 - 8 °C (stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eThis is an indirect-competitive ELISA kit for quantitative determination of kanamycin residues in plasmid DNA and related biologic samples (including cell and gene therapy products). Microplate wells are coated with kanamycin antigen which competes with kanamycin in the sample for a monoclonal anti-kanamycin antibody. An enzyme-tagged secondary antibody binds the primary antibody; addition of TMB chromogenic substrate yields a colorimetric signal at 450 nm that is inversely proportional to kanamycin concentration. Total kanamycin is determined from a standard curve (4-parameter logistic) and adjusted by sample dilution factor. Sample suitability testing is recommended for other biologic matrices to assess matrix interference.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eIndirect-competitive ELISA, 96-well plate (8×12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e\u0026lt; 0.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 ng\/mL - 16 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAccuracy (Spike recovery)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e80% - 120%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePrecision\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCoefficient of variation (CV) \u0026lt; 20%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCross-reactivity\u003c\/td\u003e\r\n                        \u003ctd\u003eKanamycin 100%; Streptomycin \u0026lt;1%; Ampicillin \u0026lt;1%; Chloramphenicol \u0026lt;1%; Tetracycline \u0026lt;1%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve \u0026amp; modeling\u003c\/td\u003e\r\n                        \u003ctd\u003eStandard curve built from ST1-ST7; concentrations: 0.5–32 ng\/mL (serial dilutions); 4-parameter logistic regression (4PL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended incubation temperature\u003c\/td\u003e\r\n                        \u003ctd\u003e25 °C ± 3 °C (optimum)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at 2 - 8 °C; overall kit stable for up to 12 months. Working solutions prepared fresh and used same day.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit below 0.5 ng\/mL, suitable for trace-level kanamycin residue analysis.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eGood accuracy and precision:\u003c\/strong\u003e Spike recovery 80–120% and CV \u0026lt;20% across tested runs.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Minimal cross-reactivity with other common antibiotics (most \u0026lt;1%).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete 96-test kit:\u003c\/strong\u003e Includes kanamycin-coated plate, standards, primary and enzyme-conjugated secondary antibodies, substrate and buffer reagents for 96 determinations.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Standardized procedures with 4-parameter logistic quantitation and recommended plate handling to support reproducible results.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOptimized for biologics:\u003c\/strong\u003e Specifically intended for kanamycin quantitation in plasmid DNA and cell\/gene therapy product workflows; sample suitability testing recommended for other matrices.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of kanamycin residues in plasmid DNA\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control of cell and gene therapy products for residual kanamycin\u003c\/li\u003e\r\n                    \u003cli\u003eResearch studies requiring measurement of kanamycin contamination in biologic preparations\u003c\/li\u003e\r\n                    \u003cli\u003eScreening of other biologic matrices for kanamycin after sample suitability testing\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard (PNB001) — 2 bottles\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard Reconstitution Diluent (PNC001) — 2 × 1.5 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin Standard Solution Diluent (PNE001) — 1 × 20 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× Kanamycin Primary Antibody (PNG001) — 1 × 60 μL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e100× Enzyme-conjugated Secondary Antibody (PNH001) — 1 × 120 μL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eKanamycin-coated Plate (PNA001) — 8 wells × 12 strips\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e10× Wash Buffer (PNJ001) — 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eChromogenic Substrate (TMB) (PND001) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI001) — 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) — 2 pieces\u003c\/span\u003e\u003cstrong\u003e2 - 8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/kit | Method: ELISA (indirect-competitive)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO); not for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at 2 - 8 °C. Do not freeze any kit components.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect kanamycin standard, secondary antibody and chromogenic substrate from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare kanamycin standard solutions and enzyme-conjugated secondary antibody working solutions on the day of use; do not store working solutions for reuse.\u003c\/p\u003e\r\n                    \u003cp\u003e• Allow kit to equilibrate at room temperature (≈25 °C) for 30 minutes before use; mix reagents thoroughly and avoid foaming.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain incubation temperature at 25 ± 3 °C; temperatures below 20 °C reduce absorbance and sensitivity.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not allow wells to dry between steps; proper washing is critical—wash 4 times with 350 μL 1× wash buffer per well.\u003c\/p\u003e\r\n                    \u003cp\u003e• Return unused strips to original foil bag with desiccant and seal tightly; strips stored at 2 - 8 °C up to 30 days after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop solution is caustic—wear eye protection and avoid contact with skin or eyes.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use kit past expiration date and do not mix reagents from different lots.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1401402.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1401402.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are recommended for this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation of kanamycin residues in plasmid DNA and is suitable for cell and gene therapy product samples. For other biologic matrices, run a sample suitability (matrix interference) test and use spiked recovery to validate the assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe detection limit is \u0026lt; 0.5 ng\/mL and the working detection range is 1 ng\/mL to 16 ng\/mL (quantitation using the standard curve).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should standards and working solutions be prepared and stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare the kanamycin stock and serial standards using the provided diluents per the manual. Stock standard solution and working solutions should be prepared fresh on the day of use; stock standard can be stored at 2-8 °C up to 30 days if protected from light. Do not store working solutions for reuse.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How is the result calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCalculate absorbance (%) relative to the zero standard, construct a standard curve (4-parameter logistic) from ST1–ST7, derive sample concentration from the curve and multiply by dilution factor. Note OD is inversely correlated with kanamycin concentration.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — not intended for clinical use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189174624437,"sku":"1401402","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/KanamycinELISAKit.jpg?v=1742291778"},{"product_id":"bovine-serum-albumin-bsa-elisa-kit-shentek®","title":"Bovine Serum Albumin (BSA) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n   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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit; calibration standard stable for 12 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSolid-phase double-antibody sandwich ELISA for quantitative detection of residual bovine serum albumin (BSA). Microtiter strips are pre-coated with affinity-purified sheep anti-BSA capture antibody. Samples and calibration standards are incubated in the coated wells, followed by a biotinylated sheep anti-BSA detection antibody and streptavidin–HRP. TMB substrate is used for color development (blue product, max absorbance ~655 nm) and reaction is stopped to yield a yellow product read at 450 nm (with 620–650 nm reference). OD450 correlates with BSA concentration and sample results are calculated using a 4-parameter logistic (4-PL) calibration curve.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003e96-well microtiter plate strips, double-antibody sandwich ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e96 tests\/Kit (8-well × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample volume per well\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1–32 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eCross-reactivity with host cell proteins (HEK293T, MDCK, CHO, BL21, Vero) \u0026lt; 0.1%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model \/ Example parameters\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL). Example: A=3.14274; B=-1.10808; C=36.73888; D=0.00859; R²=0.99999\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRead wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003ePrimary 450 nm, reference 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample storage\u003c\/td\u003e\r\n                        \u003ctd\u003eFor long-term storage, -70 °C or below recommended to avoid degradation; avoid repeated freeze-thaw cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation examples\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation 2 h RT; biotinylated antibody 45 min RT; streptavidin-HRP 30 min RT; TMB reaction 15 min RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume for dilutions\u003c\/td\u003e\r\n                        \u003ctd\u003eMinimum volume 5 µL recommended to avoid pipetting error\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 1 ng\/mL enables detection of low-level residual BSA in bioprocess samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal anti-BSA capture\/detection antibodies with cross-reactivity to common host cell proteins \u0026lt;0.1%.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use pre-coated strips:\u003c\/strong\u003e Anti-BSA coated microtiter strips supplied in sealed bag with desiccant for reduced setup time and consistent performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated 4-PL calibration:\u003c\/strong\u003e Use of 4-parameter logistic curve for accurate quantitation across the stated dynamic range.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete reagent set:\u003c\/strong\u003e Includes calibration standard, wash buffer concentrate, biotinylated antibody, streptavidin-HRP, TMB substrate and stop solution.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOptimized workflow:\u003c\/strong\u003e Standardized incubation and wash steps for reproducible results and straightforward integration into QC workflows.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual BSA in in-process samples\u003c\/li\u003e\r\n                    \u003cli\u003eMeasurement of BSA in harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and purification monitoring in biologics manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing for residual serum-derived impurities\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBSA Calibration standard (PNB007)\u003c\/span\u003e\u003cstrong\u003e1 × 500 µL; store at 2-8 °C (refer to tube label); stable up to 1 year\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-BSA Microtiter Strips (PNA007)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated, sealed in bag with desiccant; store at 2-8 °C, opened strips in bag with desiccant up to 30 days\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; dilute 1:10 with ultra-pure water to prepare 1×Wash Buffer; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-BSA: Biotinylated Conjugate (100×) (PNG007)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; biotinylated anti-BSA (sheep polyclonal) in protein matrix with preservative; sealed, keep away from light; dilute 100× in 1×Wash Buffer before use; centrifuge before use; recommended single-use, prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002)\u003c\/span\u003e\u003cstrong\u003e1 × 140 µL; streptavidin labeled with HRP; sealed, keep away from light; dilute 100× in 1×Wash Buffer before use; centrifuge before use; recommended single-use, prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND005)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; 3,3',5,5'-tetramethylbenzidine; sealed, keep away from light; equilibrate to RT 20 min before use; discard if colored\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; store at 2-8 °C; handle with appropriate PPE (goggles, gloves)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubations to prevent evaporation\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2-8 °C; check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid BSA contamination of the workspace and consumables; perform assay in BSA-free environment.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra-pure water; water temperature should not exceed 37 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it inhibits HRP and will cause underestimation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — avoid direct contact with skin, eyes and clothing; wear goggles and appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Anti-BSA:Biotinylated Conjugate and Streptavidin-HRP fresh before use and centrifuge concentrates before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless before use; discard and contact support if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not allow wells to dry during the assay; seal plate to prevent evaporation after incubations.\u003c\/p\u003e\r\n                    \u003cp\u003e• Plate reading should be completed within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended volume for accurate dilution steps is 5 µL; pipette carefully to avoid bubbles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended to validate the method for each sample matrix prior to routine testing.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1401401.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1401401.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay dynamic range and LLOQ?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 1–32 ng\/mL with an LLOQ of 1 ng\/mL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is sample concentration calculated?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSubtract NCS OD from sample OD, use a 4-parameter logistic (4-PL) regression of the calibration standards to derive concentration, then multiply by sample dilution factor.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I store opened components and prepared reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore unopened kit at 2–8 °C. Opened strips: keep sealed with desiccant at 2–8 °C up to 30 days. Calibration standard: 2–8 °C up to 1 year. Prepare diluted detection reagents fresh and use immediately; biotinylated antibody and streptavidin-HRP are recommended single-use due to stability.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with different sample matrices (process intermediates, final drug product)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — the kit is intended for in-process samples, harvested bulk, drug substance and drug product. A method validation is recommended for each matrix to confirm suitability, appropriate dilutions, and recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189190615221,"sku":"1401401","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BovineSerumAlbumin_BSA_ELISAKit.jpg?v=1742292802"},{"product_id":"porcine-trypsin-elisa-kit-shentek®","title":"Porcine Trypsin ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (kit); Reconstituted standard: \u0026lt;-20 °C (up to 90 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003ePorcine Trypsin ELISA Kit is a solid-phase, double-antibody sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for quantitative detection of residual porcine trypsin in biological products and downstream purification samples. A sheep polyclonal anti-trypsin capture antibody is coated on microtiter strips. Samples and calibration standards are added to the coated wells, followed by a biotinylated anti-trypsin detection antibody and Streptavidin-HRP. HRP catalyzes TMB oxidation to produce a colorimetric signal (read at 450 nm with a 620–650 nm reference). The kit includes lyophilized calibration standards, pre-coated strips, reagents for detection (biotinylated antibody and Streptavidin-HRP), TMB substrate and stop solution. The assay is intended for research use only to detect residual recombinant or pancreas-derived porcine trypsin in harvested bulk, in-process samples, drug substance and drug product.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eFormat\u003c\/td\u003e\r\n                        \u003ctd\u003eSandwich ELISA (solid-phase)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample volume per well\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.040–2.56 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.04 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards (nominal concentrations)\u003c\/td\u003e\r\n                        \u003ctd\u003eST1 2.56 ng\/mL, ST2 1.28 ng\/mL, ST3 0.64 ng\/mL, ST4 0.32 ng\/mL, ST5 0.16 ng\/mL, ST6 0.08 ng\/mL, ST7 0.04 ng\/mL, NCS 0 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model (example)\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL); example fit parameters provided (A=2.16282, B=-1.00528, C=1.54458, D=-0.01333, R²=0.99996)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with CHO, MDCK, Vero, HEK293, E. coli and Hansenula polymorpha\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate washing\u003c\/td\u003e\r\n                        \u003ctd\u003eUse 1× Wash Buffer; typical wash volume 340 µL\/well; wash 3 times between steps (5 times before TMB)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation times (typical per protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 1 hour at RT (500 rpm); Anti-Trypsin:Biotin incubation: 45 min at RT; Streptavidin-HRP incubation: 30 min at RT; TMB incubation: 15 min at RT; post-stop incubation: 5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate layout \/ replicates\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommend 2–3 replicates per sample and inclusion of spiked recovery controls\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage of kit\u003c\/td\u003e\r\n                        \u003ctd\u003eStore kit at 2–8 °C. Check expiration date on labels.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage of opened components\u003c\/td\u003e\r\n                        \u003ctd\u003eAnti-Trypsin microtiter strips: 2–8 °C (in bag with desiccant) up to 90 days; Reconstituted Trypsin Calibration Standard: \u0026lt; -20 °C up to 90 days (≤3 freeze-thaw cycles)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH recommendation for samples\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommended sample pH 6.0–8.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 0.04 ng\/mL and linear range down to 0.04 ng\/mL enables detection of low residual trypsin levels.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal anti-trypsin antibodies with no observed cross-reactivity to common host cell lines and microorganisms (CHO, MDCK, Vero, HEK293, E. coli, Hansenula).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit format:\u003c\/strong\u003e Includes pre-coated strips, lyophilized standards, detection reagents (biotinylated antibody, Streptavidin-HRP), TMB substrate and stop solution for a 96-test kit.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQuantitative readout:\u003c\/strong\u003e 4-parameter logistic calibration recommended for accurate quantitation; absorbance read at 450 nm with reference wavelength.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for downstream process monitoring:\u003c\/strong\u003e Suitable for testing residual recombinant porcine trypsin and pancreas-derived trypsin in in-process and final product samples.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eDetermination of residual porcine trypsin in downstream purification of biologics\u003c\/li\u003e\r\n                    \u003cli\u003eTesting harvested bulk, in-process samples, drug substance and drug product for residual trypsin\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and validation for recombinant protein production where trypsin is used (e.g., cell dissociation, activation steps)\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications requiring quantitation of porcine trypsin contamination\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTrypsin Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003ePNB006, 2 bottles; store lyophilized per label; reconstituted standard \u0026lt; -20 °C (up to 90 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003ePNA006, 8 wells × 12 strips; store in vacuumed bag with desiccant at 2–8 °C; use within 90 days after opening\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003ePNC003, 1 × 240 μL; room temperature handling; used to dissolve calibration standard\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003ePNF001, 3 × 25 mL; dilute 10× with ultra-pure water to 1×; store 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin:Biotin (100×)\u003c\/span\u003e\u003cstrong\u003ePNG006, 1 × 140 μL; centrifuge before use; dilute 100× in 1× Wash Buffer; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×)\u003c\/span\u003e\u003cstrong\u003ePNH002, 1 × 140 μL; centrifuge before use; dilute 100× in 1× Wash Buffer; prepare fresh\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003ePND002, 1 × 12 mL; equilibrate to RT 20 min before use; TMB should be colorless\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 M HCl)\u003c\/span\u003e\u003cstrong\u003ePNI002, 1 × 6 mL; corrosive — avoid contact with eyes, skin, clothing\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003ePNK001, 3 pieces; use to prevent evaporation during incubations\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit at 2–8 °C. Check expiration dates on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard: store below -20 °C up to 90 days; avoid more than 3 freeze-thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Anti-Trypsin microtiter strips: store in sealed bag with desiccant at 2–8 °C; use within 90 days after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1× Wash Buffer freshly by diluting 10× concentrate with ultra-pure water; if cloudy, warm at 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Dilute Anti-Trypsin:Biotin and Streptavidin-HRP 100× in 1× Wash Buffer immediately before use; centrifuge concentrates before dilution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use TMB substrate only if colorless; equilibrate to RT for 20 minutes before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive. Avoid contact with skin, eyes and clothing; handle in appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) because it will deactivate HRP and cause underestimation of trypsin levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommend sample pH between 6.0 and 8.0; outside this range may produce abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw of samples; long-term storage recommended at -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix different kit lots in the same assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain accurate pipetting (minimum recommended volume 5 µL for dilutions) and avoid bubbles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Trypsin Calibration Standard Solutions, Biotinylated Antibody Solution and Streptavidin-HRP Solution are recommended for single use due to stability concerns.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402422.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402422.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay's linear range and sensitivity?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.040–2.56 ng\/mL with an LLOQ of 0.04 ng\/mL (example R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I store opened strips and reconstituted standards?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore opened anti-trypsin microtiter strips in the sealed bag with desiccant at 2–8 °C for up to 90 days. Reconstituted Trypsin Calibration Standard should be stored below -20 °C for up to 90 days and subjected to no more than three freeze-thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What if I observe high background or abnormal OD values?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCommon causes include cross-contamination, insufficient washing, equipment contamination, or environmental contamination. Use freshly prepared reagents, increase wash volume or wash cycles, clean equipment with 75% ethanol, and ensure proper plate sealing and handling to reduce background.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which sample types and pH are suitable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSuitable for harvested bulk, in-process samples, drug substance and drug product. Recommended sample pH is between 6.0 and 8.0. Remove insoluble material by centrifugation or filtration and avoid repeated freeze-thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189198905525,"sku":"1402422","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BovineSerumAlbumin_BSA_ELISAKit_1da1a515-db19-44a3-aedc-3186ca1e5b60.jpg?v=1742293460"},{"product_id":"trypsin-analog-elisa-kit-shentek®","title":"Trypsin-analog ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (check label for expiration)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eOne-step, solid-phase sandwich Enzyme‑linked Immunosorbent Assay (ELISA) for quantitative detection of Trypsin-analog residuals in biological product preparations and process samples. The kit uses a sheep polyclonal capture antibody coated on microtiter strips and an HRP-conjugated sheep polyclonal anti-Trypsin-analog detection antibody added simultaneously with samples\/standards. HRP catalyzes TMB oxidation producing a colorimetric signal read at 450 nm (with long-wavelength correction 620–650 nm). The kit is suitable for detecting non-animal origin recombinant fungal Trypsin-analog and related engineered enzyme reagents (examples: TrypLE™, Recombinant Trypsin, HyrTryp™). Intended for research use only (not for clinical diagnostics).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit (8-well × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step sandwich ELISA; simultaneous addition of HRP-conjugated detection antibody and samples\/standards\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.16–10.24 ng\/mL, R² \u0026gt; 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.16 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical read wavelengths\u003c\/td\u003e\r\n                        \u003ctd\u003ePrimary: 450 nm; Reference\/long-wavelength correction: 620–650 nm\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation on thermoshaker: 600 rpm, 2 hours at room temperature; TMB substrate incubation: 15 minutes at room temperature\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration standards\u003c\/td\u003e\r\n                        \u003ctd\u003eSeven-point serial dilution prepared from supplied Calibration Standard to produce ST1–ST7: 10.24, 5.12, 2.56, 1.28, 0.64, 0.32, 0.16 ng\/mL (NCS = 0)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with common engineered cell lines (MDCK, Vero, HEK293, E. coli, P. pastoris, Sf9) and no cross-reactivity with recombinant human, bovine or porcine trypsin\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.5 (outside this range may cause abnormal results)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended long-term sample storage\u003c\/td\u003e\r\n                        \u003ctd\u003e-70 °C to avoid degradation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash buffer preparation\u003c\/td\u003e\r\n                        \u003ctd\u003eWash Buffer Concentrate (10×) dilute 1:10 with ultra-pure water (prepare fresh)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ 0.16 ng\/mL allows sensitive quantification of residual Trypsin-analog.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity to common cell lines or to recombinant human\/bovine\/porcine trypsin; designed for fungal recombinant Trypsin-analog detection.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified sheep anti-Trypsin-analog polyclonal antibody for consistent capture.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step workflow:\u003c\/strong\u003e Simultaneous addition of HRP-conjugated detection antibody and samples\/standards simplifies procedure and reduces hands-on time.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQuantitative output:\u003c\/strong\u003e Dose–response curve (4-parameter logistic) provides quantitative concentration values; compatible with standard curve software.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eDetection and quantification of residual Trypsin-analog in cell harvest solutions\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring during biologics production\u003c\/li\u003e\r\n                    \u003cli\u003eAnalysis of drug substance and drug product for residual enzyme\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and method validation for products using Trypsin-analog (e.g., TrypLE™, Recombinant Trypsin, HyrTryp™)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTrypsin-analog Calibration Standard (PNB009)\u003c\/span\u003e\u003cstrong\u003e1 × 100 μL; store at 2-8 °C (solution should be clear\/transparent); prepare fresh dilutions before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin-analog Microtiter Strips (PNA009)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated and supplied in vacuum bag with desiccant; store sealed at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE005)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e1 × 25 mL; store at 2-8 °C; may crystallize at low temperature—dissolve at 37 °C before use; dilute 1:10 with ultra-pure water\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-Trypsin-analog:HRP (100×) (PNN003)\u003c\/span\u003e\u003cstrong\u003e1 × 140 μL; sheep polyclonal antibody conjugated to HRP; store at 2-8 °C; dilute 100× in Diluent immediately before use; centrifuge briefly before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND002)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; store at 2-8 °C; equilibrate to room temperature 20 min before use; protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL; 1 M HCl; store at 2-8 °C; handle with care (avoid contact with eyes\/skin\/clothing)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: One-step sandwich ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at 2-8 °C (including opened components); check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Recommended sample pH: 6.5–8.5; samples outside this range may produce abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples; long-term sample storage at -70 °C is recommended.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra‑pure water (do not exceed 37 °C) to prepare buffers; prepare Wash Buffer fresh.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it inhibits HRP and will underestimate Trypsin-analog levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate should be colorless; discard if colored. Protect TMB from light during incubation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl—avoid contact with eyes, skin and clothing; handle with appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge Anti-Trypsin-analog:HRP(100×) before dilution\/use to avoid loss of reagent.\u003c\/p\u003e\r\n                    \u003cp\u003e• Calibration Standard Solutions and HRP-conjugated antibody are recommended for single use due to stability.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetted volume for accurate dilution: 5 μL.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402424.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402424.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay dynamic range and sensitivity?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.16–10.24 ng\/mL with an LLOQ of 0.16 ng\/mL (R² \u0026gt; 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sample types can be tested and how should they be prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eTest samples include cell harvest solutions, in-process samples, drug substance and drug product. Samples must be clear and transparent; remove insoluble material by centrifugation or filtration. Dilute samples into Diluent to bring Trypsin-analog concentration within the calibration range. Avoid repeated freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Does the kit cross-react with other trypsins or cell components?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit shows no cross-reactivity with common engineered cell lines (MDCK, Vero, HEK293, E. coli, P. pastoris, Sf9) and no cross-reactivity with recombinant human, bovine or porcine trypsin.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are calibration standards prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 100× dilute stock of the supplied Calibration Standard, then perform serial 1:1 dilutions to obtain ST1–ST7 at: 10.24, 5.12, 2.56, 1.28, 0.64, 0.32, 0.16 ng\/mL. NCS (0) is Diluent only.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO). Not for clinical use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189205721269,"sku":"1402424","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Trypsin-analogELISAKit.jpg?v=1742294003"},{"product_id":"nonspecific-endonuclease-smne-elisa-kit-shentek®","title":"Nonspecific Endonuclease (SMNE) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 4 hours\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (calibration standard stable for 12 months; microtiter strips stable for 30 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eA sandwich-format solid-phase ELISA for quantitative detection of residual nonspecific endonuclease (SMNE) in bioprocess samples. The assay uses a sheep polyclonal anti-SMNE capture antibody coated on microtiter strips, a biotinylated anti-SMNE detection antibody and streptavidin-HRP. Signal is developed with TMB substrate and read at 450 nm (with 620–650 nm reference). The kit can detect genetically engineered Serratia marcescens endonucleases (e.g., Benzonase®) and is intended for research use only (not for clinical diagnostics).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eSandwich ELISA (solid-phase double-antibody sandwich)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMeasurement wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.1–25 ng\/mL (R² ≥ 0.990)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.25 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with RNase, host cell proteins (E. coli, CHO, HEK293T, Vero cells) or BSA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL) regression. Example curve parameters provided (A=4.08707; B=-1.00169; C=30.95401; D=0.00166; R²=0.99997)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical sample types\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance, drug product (samples must be clear\/transparent; remove particulates by centrifugation\/filtration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003e5 µL minimum recommended for dilution accuracy\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate layout \/ throughput\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests per kit (8 wells × 12 strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e Sheep polyclonal antibody pair selective for SMNE; no cross-reactivity with common RNases, host cell proteins or BSA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive quantitation:\u003c\/strong\u003e Low LLOQ (0.25 ng\/mL) and linear range down to 0.1 ng\/mL for detection of residual endonuclease.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Pre-coated microtiter strips provided in a sealed bag with desiccant for convenience and reduced hands-on preparation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStandardized protocol:\u003c\/strong\u003e Complete workflow with recommended dilutions, incubation times, and 4‑PL curve fitting for reliable quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eProcess monitoring application:\u003c\/strong\u003e Designed for quantitation of SMNE in bioprocess purification stages, including detection of engineered endonucleases such as Benzonase®.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual SMNE (Serratia marcescens endonuclease) during bioprocess purification\u003c\/li\u003e\r\n                    \u003cli\u003eMonitoring endonuclease removal in in-process samples and harvested bulk\u003c\/li\u003e\r\n                    \u003cli\u003eTesting drug substance and drug product for residual endonuclease impurities\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and QC support during downstream processing (research use)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSMNE Calibration standard (PNB004) 1 × 100 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (store up to 12 months; follow label instructions)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-SMNE Microtiter Strips (PNA004) 8 wells × 12 strips\u003c\/span\u003e\u003cstrong\u003e2-8 °C in sealed bag with desiccant (store up to 30 days after opening)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSMNE Diluent (PNE003) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001) 2 × 25 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (dilute 10× → 1× with ultra-pure water prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-SMNE: Biotinylated Conjugate (100×) (PNG004) 1 × 120 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C (centrifuge before use; dilute 100× in diluent immediately prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002) 1 × 140 μL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; keep sealed and protected from light (dilute 100× in diluent immediately prior to use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND003) 1 × 12 mL\u003c\/span\u003e\u003cstrong\u003e2-8 °C; sealed and protected from light; equilibrate to room temperature 20 min before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002) 1 × 6 mL (1 M HCl)\u003c\/span\u003e\u003cstrong\u003e2-8 °C (corrosive; handle with PPE)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001) 3 pieces\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (sandwich double-antibody)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store the kit at 2–8 °C. Check individual component labels and recommended storage after opening.\u003c\/p\u003e\r\n                    \u003cp\u003e• Equilibrate kit reagents to room temperature for 20 minutes before use; return to 2–8 °C immediately after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze-thaw cycles of samples; for long-term storage use ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra-pure water; water temperature should not exceed 37 °C when preparing buffers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) — it will inactivate HRP and cause underestimation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Sorbitol may cause high background; sample sorbitol concentration should not exceed 0.01%.\u003c\/p\u003e\r\n                    \u003cp\u003e• Maintain sample pH between 6.5 and 8.5 to avoid assay interference.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Biotinylated antibody and Streptavidin-HRP dilutions fresh for each assay; recommended for single use due to stability.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive: avoid contact with eyes, skin, and clothing; wear goggles and appropriate PPE.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after termination.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402421.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402421.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitative measurement of nonspecific endonuclease (SMNE) in bioprocess samples (in-process material, harvested bulk, drug substance and drug product). It is for research use only and not for clinical diagnostics.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity and working range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLinear range 0.1–25 ng\/mL with a reported LLOQ of 0.25 ng\/mL; a 4-parameter logistic (4-PL) regression is used for quantitation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit detect Benzonase® or other engineered Serratia marcescens endonucleases?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes — the kit is designed to detect genetically engineered endonucleases from Serratia marcescens, such as Benzonase®.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store opened components and how long are they stable?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the kit at 2–8 °C. Calibration standard is stable at 2–8 °C for up to 12 months (check tube label); opened microtiter strips stored with desiccant at 2–8 °C are stable up to 30 days. Other reagents should be used according to label and prepared fresh where indicated.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO); not intended for clinical use.\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189209161909,"sku":"1402421","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/NonspecificEndonuclease_SMNE_ELISAKit.jpg?v=1742294517"},{"product_id":"deoxyribonuclease-i-dnase-elisa-kit-shentek®","title":"Deoxyribonuclease I (DNase) ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (opened strips stable for 90 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK Deoxyribonuclease I (DNase I) ELISA Kit is a 96-well, sandwich-format immunoassay for quantitative measurement of DNase I impurities in bioprocess samples and finished products (e.g., mRNA vaccines and therapeutics). The assay uses sheep polyclonal anti-DNase I capture antibody coated on microtiter strips, a biotinylated anti-DNase I detection antibody, and streptavidin-HRP for signal generation. TMB substrate is oxidized by HRP and measured at 450 nm (with 620–650 nm reference); concentration is determined using a 4-parameter logistic (4-PL) calibration curve. The kit is intended for Research Use Only (RUO) and supports testing from in-process samples through drug substance and drug product.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay principle\u003c\/td\u003e\r\n                        \u003ctd\u003eDouble-antibody sandwich ELISA (capture antibody coated strips; biotinylated detection antibody; streptavidin-HRP; TMB substrate)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.5–32 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower limit of quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e0.5 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration curve model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic (4-PL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical 4-PL parameters (example)\u003c\/td\u003e\r\n                        \u003ctd\u003eA = 2.50521; B = -0.70719; C = 20.59525; D = -0.04360; R² = 0.99946\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMeasurement wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay incubation times (per protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003eSample incubation: 1 hour (RT, 400–600 rpm); Biotinylated antibody: 45 min (RT); Streptavidin-HRP: 30 min (RT); TMB reaction: 15 min (RT); Post-stop incubation: 5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with T7 RNA Polymerase, RNase Inhibitor, BsaI; no cross-reactivity with host cell proteins from P. pastoris, MDCK, Vero, HEK293, Sf9, CHO, E. coli\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample matrix \/ intended samples\u003c\/td\u003e\r\n                        \u003ctd\u003eIn-process samples, harvested bulk, drug substance and drug product (e.g., mRNA production workflows)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptable sample pH\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.5–8.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMinimum recommended pipetting volume\u003c\/td\u003e\r\n                        \u003ctd\u003e5 µL (recommended minimum for dilutions)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage\u003c\/td\u003e\r\n                        \u003ctd\u003eStore kit at 2–8 °C; protect light-sensitive components (TMB, streptavidin-HRP, biotinylated conjugate)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity and defined quantitation range:\u003c\/strong\u003e LLOQ 0.5 ng\/mL with linear range 0.5–32 ng\/mL and high curve fit (R² ≈ 0.999)\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh specificity:\u003c\/strong\u003e No cross-reactivity with key enzymes used in mRNA production (T7 RNA Polymerase, RNase inhibitor, BsaI) or common host cell proteins\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use coated strips:\u003c\/strong\u003e Microtiter strips pre-coated with affinity-purified sheep anti-DNase I capture antibody for consistent plate-to-plate performance\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete reagent set for ELISA:\u003c\/strong\u003e Includes calibration standard, diluent, wash buffer concentrate, biotinylated detection antibody, streptavidin-HRP, TMB substrate, and stop solution\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for bioprocess monitoring:\u003c\/strong\u003e Suitable for quantitation of DNase I from in-process samples to final drug product, including mRNA workflows\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of DNase I impurities during mRNA vaccine and therapeutic production\u003c\/li\u003e\r\n                    \u003cli\u003eTesting in-process samples, harvested bulk, drug substance and drug product\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and release testing (research use)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and spike-recovery studies for DNase I monitoring\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNase I Calibration Standard (PNB016)\u003c\/span\u003e\u003cstrong\u003e1 × 350 µL; store at 2–8 °C; refer to tube label for details\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-DNase I Microtiter Strips (PNA016)\u003c\/span\u003e\u003cstrong\u003e8-well × 12 strips; strips pre-coated and packaged with desiccant; store in sealed bag at 2–8 °C (opened strips stable up to 90 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (PNE008)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (PNF001)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL; store at 2–8 °C; dilute 1:10 with ultrapure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-DNase I: Biotinylated Conjugate (100×) (PNG008)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL; sealed, protect from light; store at 2–8 °C; dilute 1:100 with Diluent fresh before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (PNH002)\u003c\/span\u003e\u003cstrong\u003e1 × 140 µL; sealed, protect from light; store at 2–8 °C; dilute 1:100 with Diluent fresh before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (PND004)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL; sealed, protect from light; equilibrate to room temp 20 min before use; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (PNI002)\u003c\/span\u003e\u003cstrong\u003e1 × 6 mL (1 M HCl); store at 2–8 °C; handle with goggles and protective equipment\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (PNK001)\u003c\/span\u003e\u003cstrong\u003e3 pieces; use to cover strips during incubations; store at 2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (not for clinical or diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit and components at 2–8 °C. Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Handle Stop Solution (1 M HCl) with care; avoid contact with eyes\/skin; wear goggles and protective clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive reagents (TMB, streptavidin-HRP, biotinylated conjugate) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples; for long-term storage keep samples at ≤ −70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Samples should have pH between 6.5 and 8.0; values outside this range may cause abnormal results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultrapure water (temperature below 37 °C) for reagent preparation; if diluent or wash buffer has precipitate, heat to 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume is 5 µL for dilution accuracy; avoid bubbles and foaming when pipetting.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge concentrated conjugates (100×) before use and prepare working solutions fresh; single-use recommended for some prepared solutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not use samples containing sodium azide (NaN3) as it will inactivate HRP and underestimate DNase I levels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Complete plate reading within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1402428.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1402428.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity and dynamic range?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 0.5–32 ng\/mL with an LLOQ of 0.5 ng\/mL. Typical calibration fits a 4-parameter logistic model (example R² ≈ 0.999).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should samples be prepared and stored prior to testing?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSamples must be clear\/transparent; remove insoluble material by centrifugation or filtration. Avoid repeated freeze–thaw cycles; for long-term storage store at ≤ −70 °C. Adjust sample dilution to fit the calibration curve and ensure pH between 6.5–8.0.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can this assay cross-react with enzymes used in mRNA production or common host cell proteins?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo. The kit has been tested and shows no cross-reactivity with T7 RNA Polymerase, RNase Inhibitor, BsaI, or host cell proteins from P. pastoris, MDCK, Vero, HEK293, Sf9, CHO, and E. coli.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe high background signal?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include reagent or equipment contamination, insufficient washing, or environmental contamination. Use freshly prepared reagents, increase wash volume\/times, clean equipment with 75% ethanol, and avoid cross-contamination of wells.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":45189219909813,"sku":"1402428","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DeoxyribonucleaseI_DNase_ELISAKit.jpg?v=1742295412"},{"product_id":"cortisol-competitive-elisa-kit","title":"Cortisol Competitive ELISA Kit","description":"\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCortisol Competitive ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSpecie\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003ehuman,mouse,rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003esandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.5 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSerum, plasma, cell culture supernatant, and other biological samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Amount\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e66.43 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRange Standard\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.12 ng\/mL – 15 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRecovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e80% – 130%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAverage Recovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e118%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eIntra Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4.3% – 4.6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eInter Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e6.4% – 7.8%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003ePlate Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDetachable 96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePlatform\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eComponents\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Competitive monoclonal antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eCortisol competitive freeze-dried standard\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eCompetitive detect Antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStandard Diluent\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eAssay Buffer(10×)\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSubstrate TMB\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eStorage\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003eIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eShipping: \u003c\/strong\u003e4℃ blue ice transportation\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/multisciences\/pdf\/EK8100-AP4.pdf\"\u003e\u003cstrong\u003eCortisol Competitive ELISA Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"multisciences","offers":[{"title":"48T","offer_id":45206773235893,"sku":"EK8100-48T","price":320.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45206773268661,"sku":"EK8100-96T","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK810096t_9f4b2492-21f7-4ff0-a2b0-a0c28f016ffa.jpg?v=1756894336"},{"product_id":"hcg-elisa-kit","title":"hCG ELISA Kit","description":"\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003ehCG ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSpecie\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003ehuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003esandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.5 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSerum, plasma, cell culture supernatant, and other biological samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Amount\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e80 μL 1×Assay Buffer and 20 μL sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4.71 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRange Standard\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e78.1 pg\/mL – 5000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRecovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e95% – 119%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAverage Recovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e108%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eIntra Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.9% – 4.7%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eInter Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.8% - 4.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003ePlate Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDetachable 96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePlatform\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eComponents\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-hCG monoclonal antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eHuman hCG freeze-dried standard\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003ehCG detect Antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStandard Diluent\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eAssay Buffer(10×)\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSubstrate TMB\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStop Solution\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eWashing Bu\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eStorage\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003eIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eShipping: \u003c\/strong\u003e4℃ blue ice transportation\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/multisciences\/pdf\/EK1299-AP4.pdf\"\u003e\u003cstrong\u003ehCG ELISA Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"multisciences","offers":[{"title":"48T","offer_id":45206782771381,"sku":"EK1299-48T","price":320.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45206782804149,"sku":"EK1299-96T","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK1299-1.png?v=1742817291"},{"product_id":"glucagon-elisa-kit","title":"Glucagon ELISA Kit","description":"\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGlucagon ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eHgnc ID\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4191\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSpecie\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003ehuman,mouse,rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003esandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.5 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSerum, plasma, cell culture supernatant, and other biological samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Amount\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e8.42 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRange Standard\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e78.13 pg\/mL – 5000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRecovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e80% – 127%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAverage Recovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e102%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eIntra Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5% – 3.7%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eInter Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4.4% – 6.2%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003ePlate Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDetachable 96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePlatform\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eComponents\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Glucagon monoclonal antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eGlucagon freeze-dried standard\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eGlucagon detect Antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStandard Diluent\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eAssay Buffer(10×)\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSubstrate TMB\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStop Solution\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eW\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eStorage\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003eIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eShipping: \u003c\/strong\u003e4℃ blue ice transportation\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/multisciences\/pdf\/EK9125-AP4.pdf\"\u003e\u003cstrong\u003eGlucagon ELISA Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"multisciences","offers":[{"title":"48T","offer_id":45206870261941,"sku":"EK9125-48T","price":320.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45206870294709,"sku":"EK9125-96T","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK9125-1.png?v=1742818472"},{"product_id":"guinea-pig-il-6-elisa-kit","title":"Guinea pig IL-6 ELISA Kit","description":"\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGuinea pig IL-6 ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eHgnc ID\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e6018\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003esandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3.5 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSerum, plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSample Amount\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSerum, plasma: 20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.74 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRange Standard\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e7.813 pg\/mL - 500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eRecovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e83% - 118%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eAverage Recovery\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e107%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eIntra Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4.3% - 6.8%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003eInter Precision\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5.9% - 9.6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 25%; height: 3px;\"\u003ePlate Type\u003c\/td\u003e\n\u003ctd style=\"width: 75%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDetachable 96-well Plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch2\u003eComponents\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e1× Washing Buffer\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e1× Assay Buffer\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStop Solution\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSubstrate TMB\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eDetect Antibody\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStandard Diluent\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStreptavidin-HRP\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStandard\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eMicroplate Wells\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eStorage\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003eIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eShipping: \u003c\/strong\u003e4℃ Blue Ice Transport\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/multisciences\/pdf\/EK406-AP4.pdf\"\u003e\u003cstrong\u003eGuinea pig IL-6 ELISA Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"multisciences","offers":[{"title":"48T","offer_id":45206907945141,"sku":"EK406-48T","price":320.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45206907977909,"sku":"EK406-96T","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK406-1.jpg?v=1742819154"},{"product_id":"progesterone-elisa-kit-1","title":"Progesterone ELISA kit","description":"\u003cdiv class=\"product-details-block wp-block-enzo-product-details\"\u003e\n\u003ch2\u003eDescriptions\u003c\/h2\u003e\n\u003cp\u003eThe Progesterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in \u0026lt; 3 hours. Absorbance is read at 405 nm. This non-radioactive enzyme immunoassay is safe and reliable with ready-to-use liquid color-coded reagents to reduce errors.\u003c\/p\u003e\n\u003cp\u003eProgesterone is the major female sex hormone, and is responsible for reproductive-related activities such as breast glandular development, the endometrial aspects of the menstrual cycle, and the establishment and maintenance of pregnancy. In addition, progesterone also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregulation, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures (pCO\u003csub\u003e2\u003c\/sub\u003e). Progesterone is secreted in large amounts by the corpus luteum and by the extracellular conversion of cholesterol, cholestryl esters, adrenal steroids, prenenolone and pregnenolone sulfate. Small quantities are also secreted directly from the adrenal glands.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/900-011-Kit-Graph_02fb9751-870b-4df3-9d59-fc3c17699d6b.webp?v=1743068454\"\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cbr\u003e\u003c\/h2\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cdiv class=\"product-details-block wp-block-enzo-product-details\"\u003e\n\u003cul\u003e\n\u003cli\u003eHighly specific and sensitive ELISA kit with results in \u0026lt; 3h\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity, measuring as little as 8.57 pg\/ml of progesterone\u003c\/li\u003e\n\u003cli\u003eHighly specific with negligible cross-reactivity to other steroids\u003c\/li\u003e\n\u003cli\u003eHigh throughput format with results in \u0026lt; 3 hours for up to 38 samples in duplicate\u003c\/li\u003e\n\u003cli\u003eEasy-to-use liquid color-coded reagents reduce errors\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: none;\" id=\"highlighter--hover-tools\"\u003e\n\u003cdiv id=\"highlighter--hover-tools--container\"\u003e\n\u003cdiv title=\"Copy\" class=\"highlighter--icon highlighter--icon-copy\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv title=\"Change Color\" class=\"highlighter--icon highlighter--icon-change-color\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv title=\"Delete\" class=\"highlighter--icon highlighter--icon-delete\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" width=\"312\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eApplication\u003c\/td\u003e\n\u003ctd width=\"312\" style=\"width: 50%;\"\u003eColorimetric detection, ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eApplication Notes\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eFor the quantitative determination of progesterone in culture supernatants, serum, and saliva from any species. Cited sample type includes plasma.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eAssay Time\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e\u0026lt;3 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eContents\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eGxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e8.57 pg\/ml (range 15.62 – 500 pg\/ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eSpecies Reactivity\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eSpecies independent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" style=\"height: 13.5pt; width: 50%;\" class=\"et3\"\u003eWavelength\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003e405 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" width=\"312\" style=\"height: 13.5pt; width: 50%;\" class=\"et4\"\u003eLong Term Storage\u003c\/td\u003e\n\u003ctd width=\"312\" style=\"width: 50%;\" class=\"et5\"\u003e+4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 13.50pt;\"\u003e\n\u003ctd height=\"18\" width=\"312\" style=\"height: 13.5pt; width: 50%;\" class=\"et4\"\u003eRegulatory Status\u003c\/td\u003e\n\u003ctd width=\"312\" style=\"width: 50%;\" class=\"et5\"\u003eRUO – Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv style=\"display: none;\" id=\"highlighter--hover-tools\"\u003e\n\u003cdiv id=\"highlighter--hover-tools--container\"\u003e\n\u003cdiv title=\"Copy\" class=\"highlighter--icon highlighter--icon-copy\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv title=\"Change Color\" class=\"highlighter--icon highlighter--icon-change-color\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv title=\"Delete\" class=\"highlighter--icon highlighter--icon-delete\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"multisciences","offers":[{"title":"ADI900011","offer_id":45216855490741,"sku":"ADI900011","price":391.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/900-011-Kit-Graph_32110aa3-8b73-4b32-b399-eeb06d18ef04.webp?v=1743068907"},{"product_id":"bovine-e2-estradiol-elisa-kit","title":"Bovine Estradiol ELISA Kit (Colorimetric)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis kit recognizes Bovine Estradiol in samples. No significant cross-reactivity or interference between Bovine Estradiol and analogues was observed.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 274.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" width=\"72\" style=\"height: 19.6px; width: 39.0259%;\"\u003eSuitable Sample Type\u003c\/td\u003e\n\u003ctd width=\"367\" style=\"width: 60.9741%; height: 19.6px;\"\u003eSerum and plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" width=\"72\" style=\"height: 19.6px; width: 39.0259%;\"\u003eStandard Curve Range\u003c\/td\u003e\n\u003ctd width=\"367\" style=\"width: 60.9741%; height: 19.6px;\"\u003e15.63-1000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eSensitivity\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003e5.87 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eAssay Type\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003eCompetitive-ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eInter-Assay\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003e%CV \u0026lt; 5.63\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eIntra-Assay\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003e%CV \u0026lt; 6.87\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eSpike Recovery\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003e85-102%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003e50 uL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eKit Type\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003eELISA Kit (Colorimetric)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eDilutions\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.6px;\"\u003e\n\u003ctd height=\"18\" style=\"height: 19.6px; width: 39.0259%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60.9741%; height: 19.6px;\"\u003eStorage of components varies.  See protocol for specific instructions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.8px;\"\u003e\n\u003ctd height=\"54\" style=\"height: 58.8px; width: 39.0259%;\"\u003eAlternate Names\u003c\/td\u003e\n\u003ctd width=\"367\" style=\"width: 60.9741%; height: 58.8px;\" class=\"et2\"\u003e17-Beta-Estradiol;17-beta-HSD\u003cbr\u003eEstradiol\u003cbr\u003eEstrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eConcentrated HRP Linked Detection Ab (100x)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConcentrated Wash Buffer (25x)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHRP Linked Ab Diluent\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMicro ELISA Plate (Dismountable)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePlate Sealer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProduct Manual\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReference Standard\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSample Diluent\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStop Solution\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSubstrate Reagent\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.\u003c\/p\u003e","brand":"wytsci","offers":[{"title":"1 kit","offer_id":45220282564789,"sku":"BFNBP323540","price":617.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Bovine_Estradiol_ELISA_Kit_Colorimetric_1.jpg?v=1743139980"},{"product_id":"hiv-1-p24-elisa-kit","title":"HIV-1 p24 ELISA Kit","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eThe gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins, which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSpecification: 96 Test\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSensitivity: 0.64 pg\/ml (50 μl); 2.62 pg\/ml (10 μl)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStandard Curve Range: 3.13–200 pg\/ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStandard Curve Gradient: 7 Points\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNumber of Incubations: 2\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSample Volume: 50 μl \/ 10 μl\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAssay Type: Sandwich ELISA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOperation Duration: 120 min\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eTypical data\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HIV-1_p24_ELISA_Kit_2_480x480.jpg?v=1756366439\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003epg\/ml\u003c\/td\u003e\n\u003ctd colspan=\"2\" style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eO.D.\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAverage\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eCorrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.00\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0190\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0200\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0195\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3.13\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0300\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0310\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0305\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e6.25\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0520\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0500\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0510\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0315\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e12.50\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.1150\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.1140\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.1145\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.0950\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e25.00\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.2920\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.2800\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.2860\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.2665\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e50.00\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.7590\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.7000\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.7295\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.7100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e100.00\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1.8220\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1.7240\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e0.7295\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1.7535\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e200.00\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3.2790\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1.7240\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3.1850\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e3.1655\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003ePrecision\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 42.8571%; text-align: center; vertical-align: middle;\" colspan=\"3\"\u003eIntra-assay Precision\u003c\/td\u003e\n\u003ctd style=\"width: 42.8571%; text-align: center; vertical-align: middle;\" colspan=\"3\"\u003eInter-assay Precision\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\" rowspan=\"2\"\u003eSample Number\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS1\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS2\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS3\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS1\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS2\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eS3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e22\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e22\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e22\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eAverage(pg\/ml)\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e4.6\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e18.4\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e58.9\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e4.2\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e19.5\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e59.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eStandard Deviation\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e4.6\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e0.59\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e4.1\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e0.21\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e0.72\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e3.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003eCoefficient of Variation(%)\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e2.6\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e3.2\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e6.9\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e5.0\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e3.7\u003c\/td\u003e\n\u003ctd style=\"width: 14.2857%; text-align: center; vertical-align: middle;\"\u003e6.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eIntra-assay Precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (precision between assays): Three samples of known concentration were tested six times on different plates to assess inter-assay precision.\u003c\/p\u003e\n\u003ch2\u003eSpike Recovery\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eSpike recovery was evaluated by spiking three levels of HIV-1 p24 into serum samples. Unspiked serum was used as a blank in this experiment.\u003c\/p\u003e\n\u003cp\u003eThe recovery ranged from 94% to 105%, with an overall mean recovery of 98%.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at 4 °C. Please refer to the protocols for detailed instructions.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eResearch Use Only\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eFor research purposes only. Not intended for use in diagnostic procedures.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/e365ba686ca95297e09fa267c282bf61_1.pdf?v=1756374728\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eHIV-1 p24 ELISA Kit\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"AREX","offers":[{"title":"96T","offer_id":51622768836789,"sku":"AEH0078","price":773.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HIV-1p24ELISAKit.jpg?v=1756366328"},{"product_id":"cho-k1-hcp-elisa-kit-one-step-elisa-shentek®-1","title":"CHO K1 HCP ELISA Kit (one-step ELISA) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit; opened strips stable for 60 days)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSolid-phase enzyme-linked immunosorbent assay (one-step double-antibody sandwich ELISA) for quantitative determination of residual host cell proteins (HCPs) originating from the CHO-K1 cell line. The assay uses an affinity-purified sheep polyclonal capture antibody coated on microtiter strips and an HRP-labeled anti-CHO-K1 HCP detection antibody added simultaneously with calibration standards or samples. TMB substrate is used for color development (blue at 655 nm; stopped reaction yields yellow product measured at 450 nm). Sample concentrations are calculated from a 4-parameter logistic dose-response calibration curve.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eOne-step double-antibody sandwich ELISA (pre-coated microtiter strips)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePackage size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 - 300 ng\/mL, R² ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantification (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e3 ng\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity with Sf9, HEK 293T, E.coli BL21, P. pastoris HCP\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical read wavelength\u003c\/td\u003e\r\n                        \u003ctd\u003e450 nm (reference\/correction 620–650 nm)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIncubation\u003c\/td\u003e\r\n                        \u003ctd\u003eSample\/reagent incubation: 3 hours at room temperature on thermoshaker (600 rpm); TMB development: 15 minutes at RT\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample pH range\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommended pH 6.5–8.5\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage (opened components)\u003c\/td\u003e\r\n                        \u003ctd\u003eMicrotiter strips in sealed bag with desiccant: 2–8 °C up to 60 days; Reconstituted calibration standard: aliquot and store ≤ -20 °C up to 60 days (avoid freeze-thaw)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCalibration model\u003c\/td\u003e\r\n                        \u003ctd\u003e4-parameter logistic regression\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCHO-K1 specific detection:\u003c\/strong\u003e Sheep polyclonal antibodies raised against CHO-K1 HCPs provide specific capture and detection of residual CHO-K1 host cell proteins.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOne-step ELISA workflow:\u003c\/strong\u003e Simultaneous addition of HRP-labeled detection antibody with standards\/samples simplifies setup and reduces handling steps.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-coated strips:\u003c\/strong\u003e Microtiter strips come pre-coated with affinity-purified capture antibody and packaged with desiccant for convenience and consistency.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSensitive and wide dynamic range:\u003c\/strong\u003e Validated linear range 3–300 ng\/mL with LLOQ 3 ng\/mL and R² ≥ 0.990.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMinimal cross-reactivity:\u003c\/strong\u003e No detectable cross-reactivity with common alternative expression hosts (Sf9, HEK293T, E.coli BL21, P.pastoris).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantification of residual CHO-K1 host cell proteins in process intermediates\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of harvested bulk, drug substance and drug product derived from CHO-K1 cells\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and recovery\/spike studies for HCP measurement\u003c\/li\u003e\r\n                    \u003cli\u003eResearch QC and bioprocess monitoring in development labs\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO-K1 HCP Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003eSealed bag with desiccant, store at 2-8 °C (stable up to 60 days after opening)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO-K1 HCP Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003e2 bottles, reconstitute with 500 µL Reconstitution Solution; aliquot and store ≤ -20 °C (stable up to 60 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution\u003c\/span\u003e\u003cstrong\u003e1 × 1.5 mL, room temperature equilibration before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL, store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×)\u003c\/span\u003e\u003cstrong\u003e2 × 25 mL, dilute 1:10 with ultra-pure water; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO-K1 HCP:HRP (100×)\u003c\/span\u003e\u003cstrong\u003e1 × 120 µL, affinity-purified HRP-conjugated sheep antibody; dilute 100× in Diluent before use; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL, protect from light; equilibrate to RT 20 min before use; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (sulfuric acid)\u003c\/span\u003e\u003cstrong\u003e1 × 12 mL, corrosive—avoid contact; store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film\u003c\/span\u003e\u003cstrong\u003e3 pieces, store at 2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for clinical diagnostic use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Intended for qualified laboratory personnel only.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate assay and sample dilution strategy before routine testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use single-use consumables; wipe work surfaces and equipment with 75% ethanol when appropriate.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid repeated freeze–thaw cycles of samples and standards; long-term sample storage recommended at ≤ -70 °C.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB substrate should be colorless before use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is sulfuric acid — avoid contact with eyes, skin and clothing; follow chemical safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid sodium azide (NaN3) in samples or reagents (it inactivates HRP).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Wash Buffer fresh from 10× concentrate; ensure solutions are clear (heat to 37 °C to dissolve precipitate if needed).\u003c\/p\u003e\r\n                    \u003cp\u003e• Read plate within 30 minutes after stopping the reaction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum recommended pipetting volume for dilutions: 5 µL to reduce error.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301305-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301305-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay range and sensitivity?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe validated linear range is 3–300 ng\/mL with an LLOQ of 3 ng\/mL (R² ≥ 0.990).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can this kit detect HCPs from non-CHO-K1 expression hosts?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is specifically designed for CHO-K1-derived HCPs; no cross-reactivity was observed with Sf9, HEK293T, E. coli BL21 or P. pastoris HCPs, so it is not recommended for quantifying HCPs from those hosts.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reconstituted standards be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eLong-term sample storage is recommended at ≤ -70 °C to prevent degradation. Reconstituted calibration standards should be aliquoted and stored at ≤ -20 °C and used within 60 days; avoid repeated freeze–thaw cycles.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Are there any reagents or sample components to avoid?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAvoid sodium azide in samples or reagents (it will deactivate HRP). Do not use samples outside recommended pH range (6.5–8.5) as this may cause abnormal results.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":51795516162229,"sku":"1301305-1","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CHOK1HCPELISAKit_one-stepELISA_SHENTEK_01.jpg?v=1758524930"},{"product_id":"cho-plbl-2-hcp-elisa-kit-shentek®","title":"CHO PLBL-2 HCP ELISA Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eELISA\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (store kit); strips: 2-8 °C (up to 90 days); reconstituted calibration standard: ≤ -18 °C (aliquot \u0026amp; avoid freeze-thaw)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eThis solid-phase enzyme-linked immunosorbent assay (ELISA) employs a double-antibody sandwich format to quantify CHO PLBL-2 (Phospholipase B-like 2) protein in biological samples. A CHO PLBL-2-specific capture antibody is pre-coated on microtiter strips. Calibration standards and test samples are added and incubated, followed by a biotinylated detection antibody that binds CHO PLBL-2 to form a sandwich complex. Streptavidin–HRP (SA-HRP) is then added and, after washing, TMB substrate is used to generate a colorimetric signal (blue), which is stopped to produce a yellow product with maximum absorbance at 450 nm. Absorbance (450 nm, with 620–650 nm reference subtraction) correlates with CHO PLBL-2 concentration and sample concentrations are determined using a 4-parameter logistic (4-PL) calibration curve. The kit is supplied for research use only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eSolid-phase double-antibody sandwich ELISA\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size\u003c\/td\u003e\r\n                        \u003ctd\u003e96 tests\/Kit\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinearity \u0026amp; Range\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e50–12,800 pg\/mL, R2 ≥ 0.990\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLower Limit of Quantitation (LLOQ)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e50 pg\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecificity \/ Cross-reactivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eNo cross-reactivity observed with Sf9 HCP, E. coli BL21 HCP and MDCK HCP\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical calibration curve (4-PL)\u003c\/td\u003e\r\n                        \u003ctd\u003e4-PL parameters: A = 5.33548; B = -1.02792; C = 26167.08880; D = -0.00074; R2 = 1.00000\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003ePlate reading\u003c\/td\u003e\r\n                        \u003ctd\u003eAbsorbance measured at 450 nm with reference at 620–650 nm; read within 10 minutes after stopping\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended replicates\u003c\/td\u003e\r\n                        \u003ctd\u003e2–3 replicates recommended per sample\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LLOQ of 50 pg\/mL and linear dynamic range up to 12,800 pg\/mL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDouble-antibody sandwich format:\u003c\/strong\u003e Specific capture antibody pre-coated on microtiter strips with biotinylated detection antibody and SA-HRP for robust signal generation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Pre-coated strips and concentrated conjugates provided; most reagents diluted immediately prior to use for convenience.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated specificity:\u003c\/strong\u003e No observed cross-reactivity with tested non-CHO host cell protein sources (Sf9, E. coli BL21, MDCK).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003e96-test throughput:\u003c\/strong\u003e Kit configured for 96 tests (8 × 12 strips), suitable for routine testing and method validation.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eDetection and quantitation of CHO PLBL-2 protein (HCP) in CHO cell culture supernatant\u003c\/li\u003e\r\n                    \u003cli\u003eAnalysis of in-process samples and harvested bulk from CHO processes\u003c\/li\u003e\r\n                    \u003cli\u003eTesting of drug substance and drug product for CHO PLBL-2 content\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and process development monitoring for biologics expressed in CHO systems\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO PLBL-2 Calibration Standard (lyophilized)\u003c\/span\u003e\u003cstrong\u003eLyophilized; reconstitute with 500 µL Reconstitution Solution; aliquot and store ≤ -18 °C, avoid freeze-thaw\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO PLBL-2 Microtiter Strips (pre-coated)\u003c\/span\u003e\u003cstrong\u003eSealed in bag with desiccant; store at 2-8 °C; after opening keep in bag with desiccant (up to 90 days)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eReconstitution Solution (1.5 mL)\u003c\/span\u003e\u003cstrong\u003eRoom temperature equilibration before use; store per kit storage (2-8 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDiluent (2 × 25 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; use for standard and conjugate dilutions\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer Concentrate (10×) (2 × 25 mL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; dilute 1:10 with ultra-pure water before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eAnti-CHO PLBL-2: Biotinylated Conjugate (200×) (60 µL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge before use; dilute 200× with Diluent fresh on day of assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStreptavidin-HRP (100×) (140 µL)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; centrifuge before use; dilute 100× with Diluent fresh on day of assay\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eTMB Substrate (12 mL)\u003c\/span\u003e\u003cstrong\u003eSealed, keep away from light; equilibrate to room temperature (25 ± 3 °C) for 20 min before use\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eStop Solution (1 × 6 mL, 1 M HCl)\u003c\/span\u003e\u003cstrong\u003eStore at 2-8 °C; corrosive (1 M hydrochloric acid) — avoid direct contact\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSealing Film (3 pieces)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; use to cover strips during incubations\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 96 tests\/Kit | Method: ELISA (double-antibody sandwich)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for clinical diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Intended for use by qualified technicians only.\u003c\/p\u003e\r\n                    \u003cp\u003e• Validate assay and sample suitability before routine testing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use sterile, single-use consumables; recommended to wipe benches\/equipment with 75% ethanol before and after use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not mix reagents from different lot numbers.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare 1×Biotinylated Conjugate and 1×Streptavidin-HRP fresh on the day of assay; calibrators and these working solutions are recommended for single use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Reconstituted calibration standard: aliquot and store ≤ -18 °C; avoid repeated freeze–thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• TMB Substrate must be colorless prior to use; discard if colored.\u003c\/p\u003e\r\n                    \u003cp\u003e• Stop Solution is 1 M HCl — corrosive; avoid contact with eyes, skin, and clothing and follow appropriate safety procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use fresh ultra-pure water (temperature should not exceed 37 °C).\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid drying wells before substrate incubation; seal plate to prevent evaporation.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge concentrated conjugates before dilution to avoid loss of reagent.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum pipetting volumes and careful technique recommended (minimum recommended pipetting volume: 5 µL) to reduce pipetting error.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid samples containing sodium azide (NaN3) as it will inactivate HRP and cause underestimation of HCP levels.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1301315.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1301315.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay detection range and LLOQ?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay linear range is 50–12,800 pg\/mL with a reported LLOQ of 50 pg\/mL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sample types are compatible with this kit and how should samples be prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCompatible sample types include CHO cell culture supernatant, in-process samples, harvested bulk, drug substance and drug product. Samples should be clear and free of insoluble material (centrifuge or filter if necessary) and diluted into the calibration curve range using the provided diluent. Method validation is recommended to establish dilution series and matrix suitability.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should reagents and the kit be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore the kit at 2–8 °C. Pre-coated strips should be kept sealed with desiccant at 2–8 °C (opened strips stable up to 90 days). Reconstituted calibration standard should be aliquoted and stored ≤ -18 °C and protected from repeated freeze–thaw cycles. Concentrated conjugates should be diluted fresh on the day of use.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is there cross-reactivity with other host cell proteins?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNo cross-reactivity was observed with the tested Sf9 HCP, E. coli BL21 HCP and MDCK HCP preparations per the kit data.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"96 Tests","offer_id":51795540902069,"sku":"1301315","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CHOPLBL-2HCPELISAKitSHENTEK.jpg?v=1758525775"},{"product_id":"garlic-common-latent-virus-gclv-elisa-kit","title":"Garlic Common Latent Virus (GCLV) ELISA Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe Garlic Common Latent Virus (GCLV) ELISA Kit is designed for the sensitive and specific detection of Garlic Common Latent Virus (GCLV) in infected garlic plants. This enzyme-linked immunosorbent assay (ELISA) provides a reliable serological method for identifying GCLV in plant tissue samples and is suitable for routine screening and research applications.\u003c\/p\u003e\n\u003cp\u003eGarlic common latent virus is the most prevalent garlic carlavirus and has been reported worldwide, including Asia, Europe, South America, and North America. In addition to garlic, GCLV is known to infect leek, onion, and chives. This kit enables accurate detection while minimizing cross-reactivity with related plant viruses.\u003c\/p\u003e\n\u003cp\u003eThis product is intended for research use only and is not intended for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003ch2\u003eTarget Information\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTarge\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eGarlic Common Latent Virus (GCLV)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVirus Family\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eBetaflexiviridae\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGenus\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eCarlavirus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eAlternative Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003ePreviously described as garlic latent virus (renamed to avoid confusion with Japanese GLV)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eHost Species\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eGarlic (Allium sativum), leek, onion, chives\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA). Specific antibodies are used to coat a high-binding microtiter plate, capturing GCLV antigens present in plant extracts. An enzyme-labeled antibody conjugate is then applied for detection.\u003c\/p\u003e\n\u003cp\u003eUpon addition of the chromogenic substrate, a color reaction develops in positive samples. Results can be interpreted visually or quantified using a microplate reader at 405 nm.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eEach kit contains all reagents required to perform the ELISA assay:\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCapture antibody specific for GCLV\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEnzyme-conjugated detecting antibody\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-binding ELISA microtiter plate\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePositive control (lyophilized)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNegative control (lyophilized)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCoating buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSample buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEnzyme conjugate buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWashing buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSubstrate buffer and PNP tablets\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInstruction manual\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSample Types\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eLeaf tissue (recommended)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStem\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSprout\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSeed\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBulb\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRoot\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003ePlant tissues can be extracted using the provided sample buffer at a recommended ratio of 1:10 (tissue or sap to buffer).\u003c\/p\u003e\n\u003ch2\u003ePerformance Characteristics\u003c\/h2\u003e\n\u003ch3\u003eSensitivity\u003c\/h3\u003e\n\u003cp\u003eThe sensitivity of the ELISA assay is moderately high. GCLV can be consistently detected in infected plant tissues diluted up to 1:270.\u003c\/p\u003e\n\u003ch3\u003eSpecificity\u003c\/h3\u003e\n\u003cp\u003eThe assay reacts with all tested isolates of GCLV and produces a strong signal. Optical density values at 405 nm typically range from 1.480 to 3.000, depending on virus titer.\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity was observed with other carlaviruses, including CLV, BBScV, KLV, LSV, PVM, PVS, and SLV. A weak cross-reaction was observed with CVB. No cross-reaction was detected with healthy garlic plant tissues, and background signal in negative controls is low.\u003c\/p\u003e\n\u003ch2\u003eStorage and Stability\u003c\/h2\u003e\n\u003cp\u003eAll kit components should be stored at the recommended temperature to ensure stability and performance. When stored properly, the kit should be used within six months of purchase.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DEIAPV154-MSDS.pdf?v=1770002202\"\u003e\u003cstrong\u003eGCLV-MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BFGCLV154.pdf?v=1770002155\"\u003e\u003cstrong\u003eGCLV-Data Sheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eRegulatory Status\u003c\/h2\u003e\n\u003cp\u003eFor Research Use Only (RUO).\u003c\/p\u003e\n\u003cp\u003eNot for diagnostic, therapeutic, or clinical use.\u003c\/p\u003e","brand":"wytsci","offers":[{"title":"500T","offer_id":52207359099061,"sku":"BFGCLV154-500T","price":0.0,"currency_code":"USD","in_stock":true},{"title":"1000T","offer_id":52207359131829,"sku":"BFGCLV154-1000T","price":0.0,"currency_code":"USD","in_stock":true},{"title":"5000T","offer_id":52207359164597,"sku":"BFGCLV154-5000T","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_5d422032-e494-44a9-b8f3-c522a1c2a085.jpg?v=1769996949"}],"url":"https:\/\/biofargo.com\/collections\/other-elisa-kits.oembed","provider":"Biofargo","version":"1.0","type":"link"}