{"title":"Current Sales","description":"","products":[{"product_id":"human-ifn-γ-precoated-elisa-kit-96t-cat-1110002","title":"Human IFN-γ Precoated ELISA kit-96T-BioSci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThe human IFN-γ ELISA kit is used for the in vitro quantitative detection of human IFN-γ in human serum, plasma, buffer or cell culture supernates . It can detect both natural and recombinant human IFN-γ.This kit is a pre-coated plate, which is completed by \"sandwich one step\". The incubation time of the whole process does not exceed 3 hours, and the total washing time is 6 times which greatly reduces the experimental time.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eDetection range :\u003c\/strong\u003e 12.5-400 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 5 pg\/mL\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrecision :\u003c\/strong\u003e Intra-Assay CV: ≤10%; Inter-Assay CV: ≤15%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Stored :\u003c\/strong\u003e 2 - 8 °C\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eVerified Reactivity：\u003c\/strong\u003eHuman\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eReagents Provided and Reconstitution\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 180px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 32.0039%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eReagents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eCytokine standard\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e2 vials\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eLyophilized, reconstitute with the volume of reagent indicated on the vial\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eBiotinylated antibody\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e2 vials\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eDilute 50 times in Diluent buffer（1×）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eStreptavidin-HRP\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e2 vials\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eDilute 100 times in Diluent buffer（1×）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eDiluent buffer（1×）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e3 vials\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eReady-to-use\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eWashing buffer（50×）\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e1 vial\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eDilute 50 times in distilled Water\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eTMB\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e1 vial\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eReady-to-use\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003eStop solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e1 vial\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eReady-to-use\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003ePrecoated ELISA plates\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e8×12\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eReady-to-use\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 32.0039%; text-align: center;\"\u003e\n\u003cp\u003ePlastic cover\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26.9818%; text-align: center;\"\u003e\n\u003cp\u003e4 sheets\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 41.0142%; text-align: center;\"\u003e\n\u003cp\u003eReady-to-use\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMaterial Required but not Provided\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e96-well ELISA plate (microplate spectrophotometer).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePipettes and pipette tips: 10 μL, 50 μL, 100 μL, 200 μL and 1000 μL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDistilled water, fresh absorbent paper.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVortex mixer and magnetic stirrer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eScientific Data\u003c\/h2\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003cem\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Screenshot2023-06-19121319_480x480.png?v=1687194990\" width=\"491\" height=\"312\"\u003e\u003c\/strong\u003e\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eAssay Procedure Summary\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAdd 100 μL of sample, standard or control per well\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd 50 μL of diluted Biotinylated Detection antibody to all wells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIncubate 2 hours at room temperature\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWash three times\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd 100 μL of streptavidin-HRP to all wells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIncubate 20 minutes at room temperature\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWash three times Add 100 μL of ready-to-use TMB Avoid direct exposure to light. Let the color develop for 10-30 minutes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd 100 μL Stop solution to all wells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRead at 450 nm within 15 minutes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/1110002_BioSci_Human_IFN-_Precoated_ELISA_Kit.pdf?v=1687230288\" target=\"_blank\" type=\"application\/pdf\"\u003eTechnical Data Sheet\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"96T","offer_id":42915864608949,"sku":"1110002","price":309.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_44f65ea2-df4f-460a-931a-6c4abe2050d3.jpg?v=1753176273"},{"product_id":"actsep®-cd3-cd28-separation-activation-magnetic-beads","title":"GMP Grade ActSep® Human T Cell CD3\/CD28 Activation \u0026 Expansion Magnetic Beads","description":"\u003cstyle\u003e\n  \/* =========================\n     Base: match CD3 template logic + mobile table scroll\n  ========================== *\/\n\n  .biofargo-table-wrapper{\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n    margin: 20px 0;\n  }\n\n  \/* Desktop: normal full width *\/\n  .biofargo-spec-table{\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #dee2e6;\n    background: #fff;\n    font-size: 14px;\n    min-width: 600px;\n  }\n\n  .biofargo-spec-table th,\n  .biofargo-spec-table td{\n    border: 1px solid #dee2e6;\n    padding: 12px;\n    text-align: center;\n    vertical-align: middle;\n    white-space: normal;\n    word-break: break-word;\n  }\n\n  \/* Mobile: force horizontal scroll *\/\n  @media (max-width: 600px){\n    .biofargo-table-wrapper{\n      border: 1px solid #eee;\n      border-radius: 8px;\n      padding: 6px;\n      background: #fff;\n    }\n\n    .biofargo-spec-table{\n      width: max-content !important;\n      min-width: 720px !important;\n      display: inline-table !important;\n    }\n\n    .biofargo-spec-table th,\n    .biofargo-spec-table td{\n      white-space: nowrap;\n    }\n  }\n\n  \/* Applications grid (same idea as CD3) *\/\n  .biofargo-app-grid{\n    display: grid;\n    grid-template-columns: 1fr 1fr;\n    gap: 20px;\n  }\n  @media (max-width: 640px){\n    .biofargo-app-grid{\n      grid-template-columns: 1fr;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\n\u003cp\u003eActSep® CD3\/CD28 Separation \u0026amp; Activation Magnetic Beads are suitable for separation of human CD3+ T cells, providing a simple method for activating and expanding human T cells without the need for antigen presenting cells and antigens.\u003c\/p\u003e\n\u003cp\u003eBy coupling anti-human CD3 and anti-human CD28 antibodies on magnetic beads, the primary and co-stimulatory signals needed to regulate T cell activation and expansion are provided.\u003c\/p\u003e\n\u003cp\u003eThe beads are suitable for human T cell separation, activation and expansion, CAR-T, and other T cell culture applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n\u003cth style=\"width: 35%; padding: 12px; text-align: center;\"\u003eTest Item\u003c\/th\u003e\n\u003cth style=\"width: 65%; padding: 12px; text-align: center;\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eBead Concentration\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e2 × 10\u003csup\u003e8\u003c\/sup\u003e beads\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eContent\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e1 mL \/ 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e\u0026lt; 0.5 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eReactive Species\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eStorage Temperature\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e2–8 °C (Do not freeze)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eValidity Period\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeature\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e4.5 μm particles\u003c\/strong\u003e\u003cbr\u003eUniform 4.5 μm magnetic particles provide consistent magnetic response and reproducible cell separation performance.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible specifications\u003c\/strong\u003e\u003cbr\u003eFlexible specifications support both small-scale research and scalable cell culture workflows.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh stability and safety\u003c\/strong\u003e\u003cbr\u003eHigh suspension stability and low nonspecific binding ensure safe and reliable operation during cell handling and culture.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStrict QC testing and traceability documentation\u003c\/strong\u003e\u003cbr\u003eEach batch undergoes strict quality control testing with complete traceability documentation.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eISO9001 and ISO13485 certified\u003c\/strong\u003e\u003cbr\u003eManufactured under ISO 9001 and ISO 13485 certified quality management systems.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProduction in Class B + A clean area\u003c\/strong\u003e\u003cbr\u003eProduced in Class B + A cleanroom environments to ensure controlled manufacturing conditions and product consistency.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplication\u003c\/h2\u003e\n\u003cdiv style=\"margin-top: 12px;\" class=\"biofargo-app-grid\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell activation\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eRapid activation of human CD3⁺ T cells by delivering primary and co-stimulatory signals for efficient initiation of T cell responses.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eImmunotherapy\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSuitable for immunotherapy research and development, supporting standardized T cell preparation in cell-based immune platforms.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell sorting\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eMagnetic separation and enrichment of human CD3⁺ T cells from PBMCs, providing a high-purity starting population for downstream workflows.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell separation\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eEfficient isolation of CD3⁺ T cells from heterogeneous cell populations to enable controlled activation, expansion, and functional studies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocument\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ActSep_CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1697702677\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; background-color: #0056b3; color: white; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603 Data Sheet\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603 SDS\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-BD2412005-GMP-TL603-1000.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603-1000 COA\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-BD2412009-GMP-TL603-5000.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603-5000 COA\u003c\/a\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eAttention\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5;  padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eFor research use and GMP manufacturing workflows only. Not for direct clinical administration.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"1mL","offer_id":43164731179189,"sku":"GMP-TL603-1000","price":490.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":43164731211957,"sku":"GMP-TL603-5000","price":2380.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/GMP-TL603-5000.png?v=1753150142"},{"product_id":"human-ifn-γ-precoated-elispot-kitstrips","title":"Human IFN-γ Precoated ELISPOT Kit(strips)-96T-Biosci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThis kit is a ready-to-use kit, including detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, washing buffer, pre-coating PVDF plate, which can meet the needs of ELISPOT experiments. This kit is only used for scientific research. It shall not be used for diagnosis. Read the instruction and check the components of the kit before use.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMaterials Provided\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 198px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 198px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eItem\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eBiotinylated Antibody\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e100 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eStreptavidin-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e100 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eCell Stimulant-PHA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eDilution Buffer R(1×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e10 mL×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eWashing Buffer (50×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e15 mL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Dilution\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e10 mL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅰ (20×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅱ (20×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅲ (200×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003ePrecoated Strip PVDF Plate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eSelf-provided Items\u003c\/h2\u003e\n\u003cp\u003e1. Cell culture medium (It is recommended to use serum-free medium or RPMI-1640 with 100 units\/mL Penicillin,100 μg\/mL Streptomycin and 5-10% fetal calf serum)\u003cbr\u003e2. Lymphocyte Separation Medium\u003cbr\u003e3. Clean bench\u003cbr\u003e4. CO 2 -incubator\u003cbr\u003e5. Multi-channel Pipette, pipette tips\u003cbr\u003e6. Amicroscope or an immune spot image analyzer for spot counting\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation of Reagents\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eWashing Buffer (50×): Diluted with distilled water to prepare 1×Washing buffer at the ratio of 1:50 for standby.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCell Stimulant-PHA: Sterile 1×PBS is added to resuspend lyophilized powder according to the label information on the vial.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eBiotinylated Antibody: Diluted with Dilution Buffer R (1×) (1:100).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eStreptavidin-HRP: Diluted with Dilution Buffer R (1×) (1:100).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAEC Coloring System: By referring to the following table, AEC Dilution , AEC Solution Ⅰ (20×), AEC Solution Ⅱ (20×), AEC Solution Ⅲ (200×) are mixed well in a clean container at the ratio of 180:10:10:1. The half-life of the AEC coloring solution is about 30 minutes, and the solution is prepared prior to use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 104.684%; height: 144px;\" data-mce-style=\"border-collapse: collapse; width: 104.684%; height: 144px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e\n\u003cp\u003eTotal volume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003eAEC Dilution\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅰ(20×)\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅱ(20×)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅲ(200×)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e0.9 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e2 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e1.8 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e3 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e2.7 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e150 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e150 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e15 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e3.6 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e4.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e25 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e8 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e7.2 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e40 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e9 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperation Process\u003c\/h2\u003e\n\u003ch3\u003eDay 1: Cell activation ( aseptic operation is required)\u003c\/h3\u003e\n\u003cp\u003e1. Pre-coating plate: The PVDF membrane is wetted by adding 200 μL of culture medium to the pre-coating plate, and then it shall be pulled out after standing at room temperature for 5-10 minutes.\u003cbr\u003e2. The 100 μL of cell suspension is added to each well. And the cell concentration is adjusted according to different mitogen or antigen(e.g.Using PHA to stimulate cells, the cell concentration can be 1×10 5 cells\/well,for reference\u003cbr\u003eonly). Only 100 μL of medium is added to the negative control well.\u003cbr\u003e3. The 10 μL of appropriate concentration of antigen or cell stimulant is added to each well.\u003cbr\u003e4. Incubation: After all the samples and stimulants are added, the plate is covered with lid. And the incubation is performed at an incubator with 37°C, 5% CO 2 for 16-24 hours. The specific incubation time varies due to different cell types and cytokines.\u003c\/p\u003e\n\u003ch3\u003eDay 2: Operation after incubation (aseptic operation is no longer required)\u003c\/h3\u003e\n\u003cp\u003e5. The cells and medium in the wells are shaken off; 200 μL of ice-cold deionized water is added to the wells, and placed in a refrigerator at 4°C for 10 minutes to remove the cells through low permeability and pyrolysis.\u003cbr\u003e6. The liquid in the well is shaken off; 260 μL of 1×Washing Buffer is added to each well. The well is washed 6 times, each time for 60 seconds. The plate strips are dried by turning it over and tapping it on the absorbent paper.\u003cbr\u003e7. The 100 μL of diluted Biotinylated Antibody is added to each well; the plate is covered with lid, and the incubation\u003cbr\u003eis performed at 37°C for 1 hour.\u003cbr\u003e8. Repeat Step 6.\u003cbr\u003e9. The 100 μL of diluted Streptavidin-HRP is added to each well; the plate is covered with lid, and the incubation is performed at 37°C for 1 hour.\u003cbr\u003e10. Repeat Step 6.\u003cbr\u003e11. The 100 μLof freshly prepared AEC Coloring solution is added to each well. Stand at a dark place and at room temperature for 5-30 minutes. Note: The spot shape is checked every 5-10 minutes until the spot is clear.\u003cbr\u003e12. The liquid in the well is poured; the base of the plate is uncovered, and the front and back of each experimental well and the base are washed with a large amount of distilled water. Excess water is shaken off; the plate is placed in a cool place and at room temperature.Afterwards, the base is installed after the plate is naturally dried.\u003cbr\u003e13. The spots are counted manually under microscope or by automatic ELISPOT analyzer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eWarnings \u0026amp; Precautions\u003c\/h2\u003e\n\u003ch3 style=\"text-align: left;\" data-mce-style=\"text-align: center;\"\u003eWarnings\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is only used for scientific researches. It shall not be used for diagnosis and treatment.\u003c\/li\u003e\n\u003cli\u003eDo not use kits that are beyond the expiration date or with damaged packaging.\u003c\/li\u003e\n\u003cli\u003eDo not use this kit in conjunction with the components of other kits.\u003c\/li\u003e\n\u003cli\u003eWear gloves and avoid direct contact with the skin, when using chemical reagents or biological products, especially using AEC substrates containing dimethylformamide (DMF) which is harmful to the skin and eyes. Once they are stuck to your skin, please immediately rinse with plenty of water and seek medical attention in time.\u003c\/li\u003e\n\u003cli\u003ePlease dispose of unused reagents or biological products according to local laws and regulations.\u003c\/li\u003e\n\u003cli\u003eNote: Pipetting, washing the plate, incubation time and temperature, cell conditions and other factors are related to the formation of spots, the degree of color development and the changes in the background.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"text-align: left;\"\u003ePrecautions\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eDo not touch the membrane directly with pipette tips, so as not to scratch or damage the membrane.\u003c\/li\u003e\n\u003cli\u003eAfter completion of the experiment, do not dry the plate at temperature higher than 37ºC, otherwise the membrane will be broken.\u003c\/li\u003e\n\u003cli\u003eBefore terminating the reaction, lift the base baffle and flush the base liquid to prevent the membrane background from aggravating, but please operate gently to avoid direct damage to the membrane.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThe kit is stored at 2-8ºC and can be stored stably for 12 months. The kit cannot be used after expiration, and remains sterile after opening.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct documentation\u003c\/h2\u003e\n\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/2110005_Human_IFN-_Precoated_ELISPOT_kit_strips_--C0_d841e402-abe0-4a82-82ba-1fc756506cb4.pdf?v=1697160995\" type=\"application\/pdf\" target=\"_blank\"\u003eHuman IFN-γ Precoated ELISPOT kit（strips）datasheet\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e","brand":"Dakewe","offers":[{"title":"96T","offer_id":43170192785589,"sku":"2110005","price":443.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_8b2e6086-5d61-47e4-a462-33632579fbe8.jpg?v=1753176121"},{"product_id":"hematoxylin-staining-solution","title":"Hematoxylin Staining Solution-Biosci™","description":"\u003ch2\u003eProduct Name\u003c\/h2\u003e\n\u003cp\u003eHematoxylin Staining Solution\u003c\/p\u003e\n\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cp\u003eIt is mainly used for staining the nucleus.\u003c\/p\u003e\n\u003ch2\u003eModel \u0026amp; Specification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e\u003cstrong\u003eCatalog#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e\u003cstrong\u003eModel \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e\u003cstrong\u003eDescription \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e4960111\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eHarris\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eHarris Hematoxylin\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e500mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrinciple of Procedure\u003c\/h2\u003e\n\u003cp\u003eThe nucleus in the cell has a certain degree of acidity, and it has a strong affinity with the basic stain (hematoxylin), while the cytoplasm is on the contrary, which has a certain degree of basicity and a strong affinity with the acid stain (eosin). After cell smears or tissue sections are subject to hematoxylin-eosin staining, the nucleus will be stained blue-purple by hematoxylin, cytoplasm, muscle fibers, collagen fibers, etc. will be red in varying degrees, and red blood cells will be orange-red.\u003c\/p\u003e\n\u003ch2\u003eReagent Provided\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 54px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003e\u003cstrong\u003eProduct Name \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003e\u003cstrong\u003eMain Compositions\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 36px;\"\u003eHematoxylin Staining Solution\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 36px;\"\u003eHematoxylin, Oxidant, Mordant,\u003cbr\u003eEthanol,Acetic acid and Stabilizer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Conditions and Expiry Date\u003c\/h2\u003e\n\u003cp\u003eStorage at 10℃-30℃, valid for 12 months.\u003c\/p\u003e\n\u003ch2\u003eSample Requirement\u003c\/h2\u003e\n\u003cp\u003eSmears and sections must be fully fixed, and paraffin sections must be fully deparaffinized.\u003c\/p\u003e\n\u003ch2\u003eMaterials Required But Not Provided\u003c\/h2\u003e\n\u003cp\u003eDewaxing Solution, Gradient Ethanol, Eosin Staining Solution , Differentiation Solution, Bluing Back Solution, Mounting Medium.\u003c\/p\u003e\n\u003ch2\u003eProcedures\u003c\/h2\u003e\n\u003cp\u003e1. Deparaffinize the sections for 5-10 min in xylene I, II and III respectively, and then transfer into 100% and 95% ethanol respectively for 1-3 min each;\u003c\/p\u003e\n\u003cp\u003e2. Immerse in 85% ethanol for 1-3 min and rinse with tap water for rehydration for 1-3 min;\u003c\/p\u003e\n\u003cp\u003e3. Stain in Hematoxylin Staining Solution for 3-5 min, and rinse with tap water for 1-2 min;\u003c\/p\u003e\n\u003cp\u003e4. Differentiate in Differentiation Solution for 1-10 sec; and rinse with tap water for 2min;\u003c\/p\u003e\n\u003cp\u003e5. Bluing with running water for 5-10 min; or bluing with lithium carbonate bluing solution for 1-3 min then rinse with tap water for 1-3 min;\u003c\/p\u003e\n\u003cp\u003e6. Immerse in Eosin Staining Solution (Water-soluble) for 30-60 sec; or dehydrate in 85% ethanol, then stain in Eosin Staining Solution (Alcohol-soluble) for 10-30 sec (Water washing should be avoided);\u003c\/p\u003e\n\u003cp\u003e7. Adjust color for 2 min in 95% ethanol I and II respectively;\u003c\/p\u003e\n\u003cp\u003e8. Dehydrate for 1-3 min through 100% ethanol I and II respectively, and clear in xylene I and II for 1-3 min respectively;\u003c\/p\u003e\n\u003cp\u003e9. Mount with mounting medium and coverslips, observe the result under microscope.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eInterpretation of Test Results\u003c\/h2\u003e\n\u003cp\u003eNuclei show blue purple. Cytoplasm, intercellular substance and fibrin show pink to red. And the red cells would show orange red.\u003c\/p\u003e\n\u003ch2\u003eLimitations\u003c\/h2\u003e\n\u003cp\u003eOnly for staining of cell morphology and histomorphology observation. The staining time and staining quality are affected by temperature, season, operator's experience, sample preparation and section quality.\u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eFor professional use only;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe user shall operate in strict accordance with the instructions; Work clothes, gloves and masks shall be wore when operating, and the operating environment shall be ventilated as possible.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter the smear or section is prepared, it shall be fully fixed; paraffin section shall be fully deparaffinized, otherwise it would lead to non-staining or uneven coloring, etc.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid storing the Hematoxylin at low temperature, it is easy to precipitate when it is stored at lower than 10℃ for a long time. The forming of surface oxide film and some bottom precipitate is normal after a period of use, it would be better to filtrate the solution with qualitative filter paper before use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe Differentiation Solution shall not be used for too long. The smear or section should be washed with clear water immediately when it turn pink.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStaining time can be properly shorten in summer or at higher temperature, and it can be properly prolonged in winter or at lower temperature.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter staining with Eosin, it should not be washed with ethanol for too long, otherwise it would cause decolorization of eosin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChange the gradient ethanol regularly to ensure the purity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis staining solution is for research use only and it needs to be used within the validity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDispose of all containers, waste liquid and slides in accordance with national regulations, including unused items and used items.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/4960111_Hematoxylin_Staining_Solution.pdf?v=1699346419\" type=\"application\/pdf\" target=\"_blank\"\u003eHematoxylin Staining Solution\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500mL\/Bottle","offer_id":43541643755701,"sku":"4960111","price":98.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4960111-HematoxylinStainingSolution_proc.jpg?v=1732873899"},{"product_id":"eosin-staining-solution","title":"Eosin Staining Solution-Biosci™","description":"\u003cp\u003e\u003cstrong\u003eNote:It is mainly used for staining the cytoplasm.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eModel \u0026amp; Specification\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 98.7888%; height: 54px;\" data-mce-style=\"border-collapse: collapse; width: 98.7888%; height: 54px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eCatalog#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eModel \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eDescription \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e4960211\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003eAlcohol-soluble\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003eAlcoholic solution of Eosin\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e500mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e4960221\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003eWater-soluble\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003eAqueous solution of Eosin\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e500mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eIntroduction\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe nucleus in the cell has a certain degree of acidity, and it has a strong affinity with the basic stain (hematoxylin), while the cytoplasm is on the contrary, which has a certain degree of basicity and a strong affinity with the acid stain (eosin). After cell smears or tissue sections are subject to hematoxylin-eosin staining, the nucleus will be stained blue-purple by hematoxylin, cytoplasm, muscle fibers, collagen fibers, etc. will be red in varying degrees, and red blood cells will be orange-red.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/1_97c16094-fbbc-4b3a-8ee9-b526f79ad0d5.png?v=1719362062\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/1_97c16094-fbbc-4b3a-8ee9-b526f79ad0d5.png?v=1719362062\"\u003e     \u003cimg style=\"font-size: 0.875rem;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ad1a1b7183951dc89f508a6b6c422144_96d4d679-e49b-46a1-8456-3054ade7181a.png?v=1719362062\" data-mce-style=\"font-size: 0.875rem;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ad1a1b7183951dc89f508a6b6c422144_96d4d679-e49b-46a1-8456-3054ade7181a.png?v=1719362062\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eMain Compositions\u003c\/h2\u003e\n\u003cp\u003eEosin, Ethanol, Acetic acid\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eSample Requirement\u003c\/h2\u003e\n\u003cp\u003eSmears and sections must be fully fixed, and paraffin sections must be fully deparaffinized.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProcedures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDeparaffinize the sections for 5-10 min in xylene I, II and III respectively, and then transfer into 100% and 95% ethanol respectively for 1-3 min each;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmerse in 85% ethanol for 1-3 min and rinse with tap water for rehydration for 1-3 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStain in Hematoxylin Staining Solution for 3-5 min, and rinse with tap water for 1-2 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDifferentiate in Differentiation Solution for 1-10 sec; and rinse with tap water for 2min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBluing with running water for 5-10 min; or bluing with bluing solution for 1-3 min then rinse with tap water for 1-3 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmerse in Eosin Staining Solution (Water-soluble) for 30-60 sec; or dehydrate in 85% ethanol, then stain in Eosin Staining Solution (Alcohol-soluble) for 10-30 sec (Water washing should be avoided);\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdjust color for 2 min in 95% ethanol I and II respectively;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDehydrate for 1-3 min through 100% ethanol I and II respectively, and clear in xylene I and II for 1-3 min respectively;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMount with mounting medium and coverslips, observe the result under microscope.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eMaterials Required But Not Provided\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAppropriate material is required for immunohistochemical (IHC) staining protocol, such as graded alcohols, xylene or xylene substitutes, hematoxylin staining solution, differentiation solution, bluing solution, etc.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2\u003eInterpretation of Test Results\u003c\/h2\u003e\n\u003cp\u003eNuclei show blue purple. Cytoplasm, intercellular substance and fibrin show pink to red. And the red cells would show orange red.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eFor professional use only;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe user shall operate in strict accordance with the instructions; Work clothes, gloves and masks shall be wore when operating, and the operating environment shall be ventilated as possible.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe prepared smears or sections shall be fully fixed. Paraffin sections shall be fully deparaffinized, otherwise non-staining or uneven staining will be caused.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe Differentiation Solution shall not be used for too long. The smear or section should be washed with clear water immediately when it turn pink.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe differentiation time shall not be too long, and the smears or sections shall be rinsed with clear water immediately after they turn pink.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStaining time can be properly shorten in summer or at higher temperature, and it can be properly prolonged in winter or at lower temperature.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter staining with Eosin, should not wash with ethanol for too long, otherwise it would cause decolorization of eosin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChange the gradient ethanol regularly to ensure the purity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis staining solution is for in vitro diagnostic (IVD) use only and it needs to be used within the validity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDispose of all containers, waste liquid and slides in accordance with national regulations, including unused items and used items.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eLimitations\u003c\/h2\u003e\n\u003cp\u003eOnly for staining of cell morphology and histomorphology observation. The staining time and staining quality are affected by temperature, season, operator's experience, sample preparation and section quality.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDisposal\u003c\/h2\u003e\n\u003cp\u003eWaste must be disposed of in compliance with local legislation. Take the appropriate precautions for infected material if necessary.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Conditions and Expiry Date\u003c\/h2\u003e\n\u003cp\u003eStorage at 10℃-30℃, valid for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Brochure_for_Staining_Solution.pdf?v=1699941846\" data-mce-href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Brochure_for_Staining_Solution.pdf?v=1699941846\" type=\"application\/pdf\" target=\"_blank\"\u003eBrochure for Staining Solution\u003c\/a\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e","brand":"Dakewe","offers":[{"title":"Water-soluble","offer_id":43270741590197,"sku":"4960221","price":168.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4960221-EosinStainingSolution_proc.jpg?v=1732871665"},{"product_id":"lymphocyte-separation-tube-for-human-peripheral-blood","title":"Lymphocyte Separation Tube with Prefilled Medium for Human Peripheral Blood-Biosci™","description":"\u003cstyle\u003e\n    .bf-container {\n        font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\n        color: #333;\n        line-height: 1.6;\n        max-width: 1200px;\n        margin: 0 auto;\n        padding: 20px;\n    }\n\n    \/* Section Headers *\/\n    .bf-container h2 {\n        \n        padding-left: 15px;\n        background: #f8f9fa;\n        padding-top: 10px;\n        padding-bottom: 10px;\n        margin-top: 30px;\n        font-size: 1.5rem;\n        color: #0056b3;\n        text-transform: uppercase;\n        letter-spacing: 0.5px;\n    }\n\n    \/* Highlight Box for Notes *\/\n    .bf-note-box {\n        background-color: #fff4e5;\n        border: 1px solid #ffcc80;\n        padding: 15px;\n        border-radius: 4px;\n        margin-bottom: 20px;\n    }\n\n    \/* Table Styling *\/\n    .bf-table-wrapper {\n        overflow-x: auto;\n        margin: 20px 0;\n    }\n\n    .bf-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 1rem;\n        background-color: transparent;\n    }\n\n    .bf-table th, .bf-table td {\n        padding: 12px 15px;\n        border: 1px solid #dee2e6;\n        text-align: left;\n    }\n\n    .bf-table tr:nth-child(even) {\n        background-color: #fcfcfc;\n    }\n\n    .bf-table td:first-child {\n        font-weight: bold;\n        background-color: #f8f9fa;\n        width: 25%;\n    }\n\n    \/* Images and Captions *\/\n    .bf-image-container {\n        text-align: center;\n        margin: 30px 0;\n    }\n\n    .bf-image-container img {\n        max-width: 100%;\n        height: auto;\n        border: 1px solid #eee;\n        border-radius: 8px;\n        box-shadow: 0 2px 10px rgba(0,0,0,0.05);\n    }\n\n    .bf-caption {\n        display: block;\n        font-style: italic;\n        color: #666;\n        margin-top: 10px;\n        font-size: 0.9rem;\n    }\n\n    \/* Responsive Document Section *\/\n    .bf-download-card {\n        display: flex;\n        align-items: center;\n        background: #ffffff;\n        border: 2px solid #0056b3;\n        border-radius: 8px;\n        padding: 20px;\n        margin: 20px 0;\n        transition: transform 0.2s;\n        text-decoration: none !important;\n    }\n\n    .bf-download-card:hover {\n        transform: translateY(-2px);\n        box-shadow: 0 4px 12px rgba(0,0,0,0.1);\n    }\n\n    .bf-download-icon {\n        font-size: 40px;\n        margin-right: 20px;\n        color: #0056b3;\n    }\n\n    .bf-download-text h4 {\n        margin: 0;\n        color: #0056b3;\n        font-size: 1.1rem;\n    }\n\n    .bf-download-text p {\n        margin: 5px 0 0;\n        color: #666;\n        font-size: 0.85rem;\n    }\n\n    \/* Lists *\/\n    .bf-container ul {\n        padding-left: 20px;\n    }\n\n    .bf-container li {\n        margin-bottom: 10px;\n    }\n\n    \/* Mobile Adjustments *\/\n    @media (max-width: 768px) {\n        .bf-table td:first-child {\n            width: 35%;\n        }\n        .bf-download-card {\n            flex-direction: column;\n            text-align: center;\n        }\n        .bf-download-icon {\n            margin-right: 0;\n            margin-bottom: 10px;\n        }\n    }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-container\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis product is used for cell separation and purification by density gradient sedimentation according to the differences in cell densities with the help of the separation medium and centrifugation. The cell gradient density separation medium produces a certain density gradient to pour the anticoagulant blood into the separation tube. After centrifugation, erythrocytes and granulocytes sink to the bottom of the tube; PBMCs (peripheral blood mononuclear cells, including lymphocytes and monocytes) float on the surface of the separation medium, and a small part of cells may suspend in the separation medium. Lymphocytes can be separated from the peripheral blood by absorbing the cells on the surface of the separation medium.\u003c\/p\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e\n\u003cp\u003eTo be used for separating lymphocytes from human peripheral anticoagulant blood.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe blood shall be collected at normal temperature, stored in an anticoagulant container at normal temperature (for not more than 2 hr) and separated at normal temperature, and shall not be kept in a 4℃ refrigerator.\u003c\/li\u003e\n\u003cli\u003eThe blood can be separated directly without the need for dilution. However, dilution does not influence the separation effect.\u003c\/li\u003e\n\u003cli\u003eFor a 15 ml separation tube, peripheral anticoagulant blood is not less than 3 ml; and for a 50 ml separation tube, peripheral anticoagulant blood is not less than 15 ml. If the blood is not enough, the use of the separation tube is not recommended, and the dilution of the blood cannot improve the separation effect.\u003c\/li\u003e\n\u003cli\u003eThe blood cells removed from the plasma can be poured into the separation tube directly or after being diluted with normal saline to double volume, so as to separate PBMCs, but only if there are enough erythrocytes. For a 15 ml separation tube, there are at least erythrocytes equivalent to those contained in 3 ml of blood; and for a 50 ml separation tube, there are at least erythrocytes equivalent to those contained in 15 ml of blood.\u003c\/li\u003e\n\u003cli\u003eIf fewer PBMC layer or red blood cells are observed, to avoid cell loss, the washing frequency can be appropriately reduced to once. For other situations, it is recommended to wash twice.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003ch2\u003ePackage\u003c\/h2\u003e\n\u003cdiv class=\"bf-table-wrapper\"\u003e\n\u003ctable class=\"bf-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n\u003cth style=\"text-align: center;\"\u003eSKU\u003c\/th\u003e\n\u003cth style=\"text-align: center;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"text-align: center;\"\u003eRecommended sample size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e7922112\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 ml\/vial×20 vials\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e3-6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e7922021\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e50 ml\/vial×25 vials\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15-25 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eMain Compositions\u003c\/h2\u003e\n\u003cp\u003eThe main compositions of the tube are ficoll, diatrizoic acid and PP or PE sieve plate.\u003c\/p\u003e\n\u003ch2\u003eApplicable Instrument\u003c\/h2\u003e\n\u003cp\u003eHorizontal rotor centrifuge.\u003c\/p\u003e\n\u003ch2\u003eSample Requirement\u003c\/h2\u003e\n\u003cp\u003eThe sample must be fresh anticoagulant blood which shall be collected by sterile operation and shall not be frozen or refrigerated in the processes of storage, handling and transportation.\u003c\/p\u003e\n\u003ch2\u003eProcedure\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eInspect, as shown in Figure 1 (A).\u003c\/p\u003e\n\u003cp\u003eTake out the separation tube, and observe whether there is free separation medium on the sieve plate material or air bubble below the sieve plate. If yes, centrifuge at 800 g for 1 min at 20℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePour blood, as shown in Figure 1 (B).\u003c\/p\u003e\n\u003cp\u003eThe blood sample must be anticoagulant blood without the need of dilution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCentrifuge at 800 g for 15 min at 20℃. Set relatively low acceleration and deceleration (If there are ten gears and the tenth gear is the highest, acceleration and deceleration shall be adjusted to the third).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter suctioning out a portion of the plasma, directly pour the remaining small amount of plasma, PBMC layer, and a small amount of separation medium above the sieve plate into a clean centrifuge tube.\u003c\/p\u003e\n\u003cp\u003eAs shown in Figure 1 (C), and the PBMC layer is below the plasma and above the separation medium.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWash with RPMI 1640 for 1-2 times (20℃, 250 g, 10 min).\u003c\/p\u003e\n\u003cp\u003eResuspend the lymphocytes with 0.9% (W\/V) normal saline or a suitable culture medium for use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eData Images\u003c\/h2\u003e\n\u003cdiv class=\"bf-image-container\"\u003e\n\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115101855_480x480.png?v=1700014750\" target=\"_blank\"\u003e \u003cimg style=\"cursor: zoom-in;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115101855_480x480.png?v=1700014750\"\u003e \u003c\/a\u003e \u003cspan class=\"bf-caption\"\u003eFigure 1: Process of Separating Blood with Lymphocyte Separation Tube for Human Peripheral Blood\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ch2\u003eExplanation of Test Results\u003c\/h2\u003e\n\u003cp\u003eDifferent performances of centrifuges of various brands and differences in temperature environments may have influence on the separation effect, so users can adjust the centrifugation revolution and the centrifugation time to find the best separation conditions (The specific separation conditions are determined by laboratories).\u003c\/p\u003e\n\u003ch2\u003eLimitations of Test Method\u003c\/h2\u003e\n\u003cp\u003eThe experiment requires that the temperature of the sample, the separation medium and the separation environment is (20±5)℃ under a normal atmosphere.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eThe separation tube shall be kept away from light at normal temperature.\u003c\/li\u003e\n\u003cli\u003eThe optimum separation temperature is (20±5)℃, beyond which the separation effect will be affected.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage Conditions and Period of Validity\u003c\/h2\u003e\n\u003cp\u003eKept unopened away from light at 2~30℃, valid for 2 years.\u003c\/p\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBoyum A. (1968): Separation of leucocytes from blood and bone marrow. Scand J. Clin. Lab. Invest. 21, Suppl. 97.\u003c\/li\u003e\n\u003cli\u003eHarris R. \u0026amp; Ukayiofo EV. (1969): Rapid preparation for lymphocytes for tissue typing. Lancet 327, 7615.\u003c\/li\u003e\n\u003cli\u003eBoyum, A. (1968): Isolation and removal of lymphocytes from bone marrow of rats and guinea-pigs.,Scand J. Clin. Lab. Invest., 21, Suppl.97, 91-109.\u003c\/li\u003e\n\u003cli\u003eZheng Dexian, Wu Kefu, Chu Jianxin. (1999): Modern Experimental Hematology Research Method and Technology. Peking Union Medical College Press.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003ca class=\"bf-download-card\" href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/7922021_7922112_BioSci_Lymphocyte_Separation_Tube_for_Human_Peripheral_Blood.pdf?v=1700027022\" target=\"_blank\"\u003e\n\u003cdiv class=\"bf-download-icon\"\u003e📄\u003c\/div\u003e\n\u003cdiv class=\"bf-download-text\"\u003e\n\u003ch4\u003eLymphocyte Separation Tube for Human Peripheral Blood-Prefilled-Biosci™\u003c\/h4\u003e\n\u003cp\u003eClick to download the PDF technical document (Instruction for Use)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eRelated Product Options:\u003c\/strong\u003e\n\u003cp\u003eSelect the appropriate format for your PBMC isolation workflow. Choose from separation medium, empty tubes, or ready-to-use prefilled tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px; margin-top: 20px;\"\u003e\n\u003c!-- Empty Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eEMPTY TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003ePBMC Separation Tube (Empty)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation?variant=52452569186485\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Human Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eHuman Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/human-lymphocyte-separation-medium?variant=43275893735605\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Mouse Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eMouse Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/mouse-lymphocyte-separation-medium?variant=43275988598965\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Prefilled Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003ePREFILLED TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eLymphocyte Separation Tube (Prefilled)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/lymphocyte-separation-tube-for-human-peripheral-blood?variant=43275829674165\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003e7922112-02, 7922112-01, 7922112\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"Pre-filled \/ 15 ml\/vial×20 vials","offer_id":43275829674165,"sku":"7922112","price":236.0,"currency_code":"USD","in_stock":true},{"title":"Pre-filled \/ 50 ml\/vial×25 vials","offer_id":43275829739701,"sku":"7922021","price":355.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6_648f3b53-e7e6-4411-a45d-06b8a2c92e55.jpg?v=1776839909"},{"product_id":"human-lymphocyte-separation-medium","title":"Human Lymphocyte Separation Medium-Biosci™","description":"\u003cstyle\u003e\n    .bf-container {\n        font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\n        color: #333;\n        line-height: 1.6;\n        max-width: 1200px;\n        margin: 0 auto;\n        padding: 20px;\n    }\n\n    \/* Section Headers *\/\n    .bf-container h2 {\n        \n        padding-left: 15px;\n        background: #f8f9fa;\n        padding-top: 10px;\n        padding-bottom: 10px;\n        margin-top: 30px;\n        font-size: 1.5rem;\n        color: #0056b3;\n        text-transform: uppercase;\n        letter-spacing: 0.5px;\n    }\n\n    \/* Highlight Box for Notes *\/\n    .bf-note-box {\n        background-color: #fff4e5;\n        border: 1px solid #ffcc80;\n        padding: 15px;\n        border-radius: 4px;\n        margin-bottom: 20px;\n    }\n\n    \/* Table Styling *\/\n    .bf-table-wrapper {\n        overflow-x: auto;\n        margin: 20px 0;\n    }\n\n    .bf-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 1rem;\n        background-color: transparent;\n    }\n\n    .bf-table th, .bf-table td {\n        padding: 12px 15px;\n        border: 1px solid #dee2e6;\n        text-align: left;\n    }\n\n    .bf-table tr:nth-child(even) {\n        background-color: #fcfcfc;\n    }\n\n    .bf-table td:first-child {\n        font-weight: bold;\n        background-color: #f8f9fa;\n        width: 25%;\n    }\n\n    \/* Images and Captions *\/\n    .bf-image-container {\n        text-align: center;\n        margin: 30px 0;\n    }\n\n    .bf-image-container img {\n        max-width: 100%;\n        height: auto;\n        border: 1px solid #eee;\n        border-radius: 8px;\n        box-shadow: 0 2px 10px rgba(0,0,0,0.05);\n    }\n\n    .bf-caption {\n        display: block;\n        font-style: italic;\n        color: #666;\n        margin-top: 10px;\n        font-size: 0.9rem;\n    }\n\n    \/* Responsive Document Section *\/\n    .bf-download-card {\n        display: flex;\n        align-items: center;\n        background: #ffffff;\n        border: 2px solid #0056b3;\n        border-radius: 8px;\n        padding: 20px;\n        margin: 20px 0;\n        transition: transform 0.2s;\n        text-decoration: none !important;\n    }\n\n    .bf-download-card:hover {\n        transform: translateY(-2px);\n        box-shadow: 0 4px 12px rgba(0,0,0,0.1);\n    }\n\n    .bf-download-icon {\n        font-size: 40px;\n        margin-right: 20px;\n        color: #0056b3;\n    }\n\n    .bf-download-text h4 {\n        margin: 0;\n        color: #0056b3;\n        font-size: 1.1rem;\n    }\n\n    .bf-download-text p {\n        margin: 5px 0 0;\n        color: #666;\n        font-size: 0.85rem;\n    }\n\n    \/* Lists *\/\n    .bf-container ul {\n        padding-left: 20px;\n    }\n\n    .bf-container li {\n        margin-bottom: 10px;\n    }\n\n    \/* Mobile Adjustments *\/\n    @media (max-width: 768px) {\n        .bf-table td:first-child {\n            width: 35%;\n        }\n        .bf-download-card {\n            flex-direction: column;\n            text-align: center;\n        }\n        .bf-download-icon {\n            margin-right: 0;\n            margin-bottom: 10px;\n        }\n    }\n\u003c\/style\u003e\n\n\u003cdiv class=\"bf-container\"\u003e\n \u003cdiv class=\"bf-note-box\"\u003e\n        \u003cstrong\u003eNote:\u003c\/strong\u003e\n        \u003cp\u003eThis product is mainly used for the separation of human peripheral blood lymphocytes, but also for the separation of most mammalian mononuclear cells.\u003c\/p\u003e\n    \u003c\/div\u003e    \n   \n\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cp\u003eHuman Lymphocyte Separation Medium is a sterile, endotoxin tested and ready-to-use solution. This product contains polysucrose, which accelerates erythrocyte precipitation, helps to improve the separation result of lymphocytes. The lymphocytes isolated are available for the cell culture and cytotoxicity assays.\u003c\/p\u003e\n\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"bf-table-wrapper\"\u003e\n        \u003ctable class=\"bf-table\"\u003e\n            \u003ctbody\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eUses\u003c\/td\u003e\n                    \u003ctd\u003eSeparation of human peripheral blood lymphocytes\u003cbr\u003eSeparation of most mammalian mononuclear cells\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eEndotoxin\u003c\/td\u003e\n                    \u003ctd\u003e\u0026lt; 0.5EU\/mL\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eDensity\u003c\/td\u003e\n                    \u003ctd\u003e(1.077±0.001)g\/mL (20℃)\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eOsmolality\u003c\/td\u003e\n                    \u003ctd\u003e(290±15)mOsmol\/kg\u003c\/td\u003e\n                \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n        \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003ePackage\u003c\/h2\u003e\n    \u003cdiv class=\"bf-table-wrapper\"\u003e\n        \u003ctable class=\"bf-table\"\u003e\n            \u003cthead\u003e\n                \u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n                    \u003cth style=\"text-align: center;\"\u003eCatalog Number\u003c\/th\u003e\n                    \u003cth style=\"text-align: center;\"\u003eName\u003c\/th\u003e\n                    \u003cth style=\"text-align: center;\"\u003eSpecification\u003c\/th\u003e\n                \u003c\/tr\u003e\n            \u003c\/thead\u003e\n            \u003ctbody\u003e\n                \u003ctr\u003e\n                    \u003ctd style=\"text-align: center;\"\u003e7111011\u003c\/td\u003e\n                    \u003ctd style=\"text-align: center;\"\u003eHuman Lymphocyte Separation Medium\u003c\/td\u003e\n                    \u003ctd style=\"text-align: center;\"\u003e100mL\/bottle\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd style=\"text-align: center;\"\u003e7111012\u003c\/td\u003e\n                    \u003ctd style=\"text-align: center;\"\u003eHuman Lymphocyte Separation Medium\u003c\/td\u003e\n                    \u003ctd style=\"text-align: center;\"\u003e250mL\/bottle\u003c\/td\u003e\n                \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n        \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003ePrinciple\u003c\/h2\u003e\n    \u003cp\u003eThe density of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090g\/ml, which is different from that of red blood cells, multinuclear leucocytes and platelets.The densities of red blood cells and granulocytes are relatively large(about 1.092g\/ml) and the density of platelets is between 1.030~1.035g\/ml. Therefore, Human Lymphocyte Separation Medium is used to generate a certain degree of density gradient, and the diluted whole blood is smoothly paved on the separation medium. After centrifugation, the red blood cells and granulocytes sink to the bottom of the tube due to their large density ; Since their densities are less than or equal to the density of separation medium, lymphocytes and monocytes are found at the surface of separation medium. There may also be a small amount of cells suspended in the separation medium. By sucking the cells on the surface of separation medium, mononuclear cells can be separated from peripheral blood.\u003c\/p\u003e\n\n    \u003ch2\u003eProtocol\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eFresh anticoagulant (EDTA, sodium citrate or heparin and other anticoagulants are acceptable) whole blood is taken. Dilute the whole blood with an equal volume of isotonic solution (PBS or normal saline).\u003c\/li\u003e\n        \u003cli\u003eAdd a certain volume of separation medium to the centrifuge tube. Carefully layer the diluted blood sample over the separation medium, keep clear interface between the two liquid surfaces. The volume ratio of separation medium, anticoagulant undiluted whole blood and isotonic solution (PBS or normal saline) is 1:1:1.\u003c\/li\u003e\n        \u003cli\u003eCentrifuge at 700-800g on a swing rotor for 20-30min at room temperature.The acceleration and deceleration are set to a slower speed (set to third gear if ten gears are available).\u003c\/li\u003e\n        \u003cli\u003eAfter centrifugation, the red blood cells are at bottom of the tube, the separation medium is at the middle layer, and the top layer is the plasma\/tissue homogenate layer. A thin and dense white membrane found at the plasma-separation medium interface is the mononuclear cell layer (including lymphocytes and monocytes).Transfer the white membrane layer into another centrifuge tube carefully.\u003c\/li\u003e\n        \u003cli\u003eDiluted the mononuclear cell layer to a certain volume with isotonic solution (PBS, normal saline or medium, etc.), then shake to mix well. Centrifuged at 250g on a swing rotor for 10min at room temperature, then remove the supernatant solution. Wash the cells once or twice.\u003c\/li\u003e\n        \u003cli\u003eResuspend the cells with isotonic solution (PBS, normal saline or medium, etc.) and count the cells for later use.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eProcedure\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003ePre-warm separation medium to room temperature (20±5℃) and shake gently before use. Avoid using the separation medium directly from refrigerator.\u003c\/li\u003e\n        \u003cli\u003eThe isotonic medium (PBS or 0.9% NaCl) used to dilute anticoagulated blood should be sterile, and can be replaced by RPMI-1640 medium.\u003c\/li\u003e\n        \u003cli\u003e1:1 blood dilution can reduce the coagulation of erythrocytes and increase the amount of lymphocytes harvested.\u003c\/li\u003e\n        \u003cli\u003eFor the activity of lymphocyte, blood should be separated immediately after collection. The cells harvested are peripheral blood mononuclear cells (PBMCs), including lymphocytes and monocytes.\u003c\/li\u003e\n        \u003cli\u003eLong storage of blood may cause bad result that there are more residual RBCs in the harvested lymphocytes. The time of centrifugation should be extended to improve the result.\u003c\/li\u003e\n        \u003cli\u003eIf the procedures are correct but there is no layer of PBMCs found at the interfaces after centrifugation, make sure that the blood is enough and fresh, and the separation mediumis suitable for the blood of this species animal.\u003c\/li\u003e\n        \u003cli\u003eOnce opened, please store the separation medium at 2-8℃ to avoid the change of density of the separation solution caused by liquid volatilization, which will affect the separation effect.\u003c\/li\u003e\n        \u003cli\u003eFor research use only.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eData Images\u003c\/h2\u003e\n\u003cdiv class=\"bf-image-container\"\u003e\n    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/3_8a898b16-1061-4b9f-89f9-419233e9c2d1.jpg?v=1776837623\" target=\"_blank\"\u003e\n        \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/3_8a898b16-1061-4b9f-89f9-419233e9c2d1.jpg?v=1776837623\" alt=\"PBMCs Viability\" style=\"cursor: zoom-in;\"\u003e\n    \u003c\/a\u003e\n    \u003cspan class=\"bf-caption\"\u003eFigure A: Viability and harvest quantity of PBMCs\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"bf-image-container\"\u003e\n    \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4_2342b113-8eaa-4818-85c9-334b13f483c8.jpg?v=1776837623\" target=\"_blank\"\u003e\n        \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4_2342b113-8eaa-4818-85c9-334b13f483c8.jpg?v=1776837623\" alt=\"Flow Cytometry Analysis\" style=\"cursor: zoom-in;\"\u003e\n    \u003c\/a\u003e\n    \u003cspan class=\"bf-caption\"\u003eFigure B: Flow cytometry analysis of Human Lymphocyte Separation Medium. RBC Lysis: 100 μL of fresh anticoagulant whole blood was collected and incubated with flow cytometry antibody for red splitting and flow cytometry detection. Separation Medium: The 3mL fresh anticoagulant whole blood was diluted and separated with human lymphocyte separation solution. After centrifugation at 800g, 20 °C for 20min, the PBMCs layer was washed twice and incubated with flow cytometry antibody for flow cytometry detection.\u003c\/span\u003e\n\u003c\/div\u003e\n\n    \u003ch2\u003eReferences\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eBoyumA. Scand J. Clin. Lab. Invest. 1968; 21, Suppl. 97.\u003c\/li\u003e\n        \u003cli\u003eHarris R. \u0026amp;Ukayiofo EV. Lancet.1969; 327: 7615.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eDocument\u003c\/h2\u003e\n    \u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/7111011_7111012_BioSci_Human_Lymphocyte_Separation_Medium.pdf?v=1700031597\" class=\"bf-download-card\" target=\"_blank\"\u003e\n        \u003cdiv class=\"bf-download-icon\"\u003e📄\u003c\/div\u003e\n        \u003cdiv class=\"bf-download-text\"\u003e\n            \u003ch4\u003eHuman Lymphocyte Separation Medium - Manual\u003c\/h4\u003e\n            \u003cp\u003eClick to download the PDF technical document (Instruction for Use)\u003c\/p\u003e\n        \u003c\/div\u003e\n    \u003c\/a\u003e\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eRelated Product Options:\u003c\/strong\u003e\n\u003cp\u003eSelect the appropriate format for your PBMC isolation workflow. Choose from separation medium, empty tubes, or ready-to-use prefilled tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px; margin-top: 20px;\"\u003e\n\u003c!-- Empty Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eEMPTY TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003ePBMC Separation Tube (Empty)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation?variant=52452569186485\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Human Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eHuman Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/human-lymphocyte-separation-medium?variant=43275893735605\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Mouse Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eMouse Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/mouse-lymphocyte-separation-medium?variant=43275988598965\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Prefilled Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003ePREFILLED TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eLymphocyte Separation Tube (Prefilled)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/lymphocyte-separation-tube-for-human-peripheral-blood?variant=43275829674165\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"100mL\/bottle","offer_id":43275893735605,"sku":"7111011","price":31.0,"currency_code":"USD","in_stock":true},{"title":"250mL\/bottle","offer_id":43275893801141,"sku":"7111012","price":73.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_39a19a29-3aea-47f8-a833-b1d14c4e0046.jpg?v=1776837436"},{"product_id":"mouse-lymphocyte-separation-medium","title":"Mouse Lymphocyte Separation Medium-Biosci™","description":"\u003cstyle\u003e\n    .bf-container {\n        font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\n        color: #333;\n        line-height: 1.6;\n        max-width: 1200px;\n        margin: 0 auto;\n        padding: 20px;\n    }\n\n    .bf-container h2 {\n        \n        padding-left: 15px;\n        background: #f8f9fa;\n        padding-top: 10px;\n        padding-bottom: 10px;\n        margin-top: 30px;\n        font-size: 1.5rem;\n        color: #0056b3;\n        text-transform: uppercase;\n        letter-spacing: 0.5px;\n    }\n\n    .bf-note-box {\n        background-color: #fff4e5;\n        border: 1px solid #ffcc80;\n        padding: 15px;\n        border-radius: 4px;\n        margin-bottom: 20px;\n    }\n\n    .bf-table-wrapper {\n        overflow-x: auto;\n        margin: 20px 0;\n    }\n\n    .bf-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 1rem;\n        background-color: transparent;\n    }\n\n    .bf-table th, .bf-table td {\n        padding: 12px 15px;\n        border: 1px solid #dee2e6;\n        text-align: left;\n    }\n\n    .bf-table tr:nth-child(even) {\n        background-color: #fcfcfc;\n    }\n\n    .bf-table td:first-child {\n        font-weight: bold;\n        background-color: #f8f9fa;\n        width: 25%;\n    }\n\n    .bf-image-container {\n        text-align: center;\n        margin: 30px 0;\n    }\n\n    .bf-image-container img {\n        max-width: 100%;\n        height: auto;\n        border: 1px solid #eee;\n        border-radius: 8px;\n        box-shadow: 0 2px 10px rgba(0,0,0,0.05);\n    }\n\n    .bf-caption {\n        display: block;\n        font-style: italic;\n        color: #666;\n        margin-top: 10px;\n        font-size: 0.9rem;\n    }\n\n    .bf-download-card {\n        display: flex;\n        align-items: center;\n        background: #ffffff;\n        border: 2px solid #0056b3;\n        border-radius: 8px;\n        padding: 20px;\n        margin: 20px 0;\n        transition: transform 0.2s;\n        text-decoration: none !important;\n    }\n\n    .bf-download-card:hover {\n        transform: translateY(-2px);\n        box-shadow: 0 4px 12px rgba(0,0,0,0.1);\n    }\n\n    .bf-download-icon {\n        font-size: 40px;\n        margin-right: 20px;\n        color: #0056b3;\n    }\n\n    .bf-download-text h4 {\n        margin: 0;\n        color: #0056b3;\n        font-size: 1.1rem;\n    }\n\n    .bf-download-text p {\n        margin: 5px 0 0;\n        color: #666;\n        font-size: 0.85rem;\n    }\n\n    .bf-container ul {\n        padding-left: 20px;\n    }\n\n    .bf-container li {\n        margin-bottom: 10px;\n    }\n\u003c\/style\u003e\n\n\u003cdiv class=\"bf-container\"\u003e\n\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cp\u003eMouse Lymphocyte Separation Medium is a new generation of density gradient separation medium. The main component is iodixanol, with a molecular weight of 1,550. It is a completely chemically inert, non-biotoxic iodide that does not bind with any known biological function protein, does not interfere with any cell surface membrane protein, does not inhibit enzyme activity and does not interfere with antigen-antibody responses.\u003cbr\u003eThe lymphocyte separated by this product is with high purity, good state and high yield. The operation of Mouse Lymphocyte Separation Medium is simple, easy to learn and does not need too much experience.\u003c\/p\u003e\n    \u003cp\u003eOur study showed that the number and quality of mouse’s spleen lymphocytes did not change significantly after 1 hour of exposure to the separation medium, and the subsequent ELISPOT test results were completely consistent with those of the control group.\u003c\/p\u003e\n\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"bf-table-wrapper\"\u003e\n        \u003ctable class=\"bf-table\"\u003e\n            \u003ctbody\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eProduct name\u003c\/td\u003e\n                    \u003ctd\u003eMouse Lymphocyte Separation Medium\u003c\/td\u003e\n                    \u003ctd\u003eSize\u003c\/td\u003e\n                    \u003ctd\u003e100mL\/bottle\u003cbr\u003e250mL\/bottle\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eApplication\u003c\/td\u003e\n                    \u003ctd\u003eSeparation of mouse\/rat spleen lymphocytes\u003cbr\u003eSeparation of mouse\/rat\/rabbit PBMCs\u003c\/td\u003e\n                    \u003ctd\u003eEndotoxin\u003c\/td\u003e\n                    \u003ctd\u003e\u0026lt; 0.5EU\/mL\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eDensity\u003c\/td\u003e\n                    \u003ctd\u003e(1.081±0.001) g\/mL (20 °C)\u003c\/td\u003e\n                    \u003ctd\u003eOsmolality\u003c\/td\u003e\n                    \u003ctd\u003e(280±15)mOsmol\/kg\u003c\/td\u003e\n                \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n        \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003eThe product shall be stored at 2 °C -30 °C and protected from light. Its shelf life is 2 years.\u003c\/p\u003e\n\n    \u003ch2\u003eMaterials Required But Not Provided\u003c\/h2\u003e\n    \u003cp\u003e35mm Petri dishes, the plunger of a 10mL glass syringe, 70µm cell strainer or nylon mesh with 200 meshes - cut into 90mm×90mm square (materials above are all sterile), centrifuge tubes, pipettes, tips, centrifuge with a swing-out rotor, etc.\u003c\/p\u003e\n\n    \u003ch2\u003eProtocol\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eSacrifice the mouse by cervical dislocation; dip it in 75% ethanol.\u003c\/li\u003e\n        \u003cli\u003eTake out the spleen of the mouse at a clean bench. Operation shall be performed in an aseptic condition.\u003c\/li\u003e\n        \u003cli\u003eAdd 4-5 mL Mouse Lymphocyte Separation Medium (pre-warming at room temperature and shaking before use) into a 35 mm petri dish. And grind (please refer to Figure 2 for grinding operation).\u003c\/li\u003e\n        \u003cli\u003eTransfer the cell suspension to a 15mL centrifuge tube immediately (refer to Figure 1(A)), and cover with 500-1000μl RPMI 1640 medium (keeping liquid level boundary clear). Please refer to Figure 1(B).\u003c\/li\u003e\n        \u003cli\u003eCentrifuge at 800g for 30min in a swing-out rotor at room temperature. The acceleration and deceleration are set to a slower speed (set to third gear if ten gears are available). The lymphocytes after centrifugation are shown in Figure 1(C).\u003c\/li\u003e\n        \u003cli\u003eTransfer the lymphocyte layer at the interface to a new centrifuge tube. Wash the cells with 10mL RPMI 1640 medium, and centrifuge at 250g for 10min.\u003c\/li\u003e\n        \u003cli\u003eDiscard the supernatant, resuspend the cells in culture medium and count.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cdiv class=\"bf-image-container\"\u003e\n        \u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115154610_480x480.png?v=1700034383\" target=\"_blank\"\u003e\n            \u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115154610_480x480.png?v=1700034383\" style=\"cursor: zoom-in;\"\u003e\n        \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003eCautions\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eIf the mouse is fed for a long time or the spleen is enlarged abnormally, which causes that the cell suspension after grinding shows dull red, please dilute the cells suspension with an equal volume of Mouse Lymphocyte Separation Medium and mix it well, and then proceed to step 4.\u003c\/li\u003e\n        \u003cli\u003eThe separation medium is volatile so the grinding time shall be controlled within 5 min.\u003c\/li\u003e\n        \u003cli\u003eOnce opened, please store the separation medium at 2-8℃ to avoid the change of density of the separation solution caused by liquid volatilization, which will affect the separation effect.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSeparation of the spleen lymphocyte of the rat:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cp\u003eBecause the rat’s spleen is large, only a small portion of it needs to be cut for the experiment. The method of grinding and separation is exactly the same as that for the separation of mouse’s spleen lymphocytes.\u003c\/p\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSeparation of mouse\/rat\/rabbit blood lymphocyte:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cul\u003e\n        \u003cli\u003eCollect 0.5-3mL anticoagulant blood of mouse\/rat\/rabbit, dilute it with an equal volume of RPMI 1640 medium or PBS. (Separation is more effective after hemodilution. Operation shall be performed in an aseptic condition)\u003c\/li\u003e\n        \u003cli\u003eAdd 3mL of Mouse Lymphocyte Separation Medium to a 15 mL centrifuge tube. Carefully layer the diluted blood over 3mL Mouse Lymphocyte Separation Medium in a 15mL centrifuge tube, avoid mixing the interface.\u003c\/li\u003e\n        \u003cli\u003eCentrifuge at 800g for 15-20min in a swing-out rotor at room temperature. The acceleration and deceleration are set to a slower speed (set to third gear if ten gears are available).\u003c\/li\u003e\n        \u003cli\u003eThe subsequent steps are the same as the mouse’s spleen lymphocyte separation operation.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSpecial instruction - Mouse’s spleen grinding:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cul\u003e\n        \u003cli\u003eIt is recommended to use nylon mesh, because it is more flexible.\u003c\/li\u003e\n        \u003cli\u003eIt is recommended to use the plunger of syringe to grind the spleen.\u003c\/li\u003e\n        \u003cli\u003eThe key is taking advantage of the rebound to control grinding, which can minimize the mechanical damage of the cells.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eNotes\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eControl the grinding strength to keep the nylon mesh suspended, to avoid a large number of cell death caused by direct grinding on the bottom of the petri dish.\u003c\/li\u003e\n        \u003cli\u003eThe petri dish cannot be too large, otherwise the nylon mesh cannot have effective elasticity.\u003c\/li\u003e\n        \u003cli\u003eClamp the nylon mesh with tweezers on one side to prevent the nylon net from sliding during grinding.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eReferences\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eLili Liu, et al.American Journal of Nephrology. 2012; 36(2): 386-396\u003c\/li\u003e\n        \u003cli\u003eLi Zhang, et al. Iranian Journal of Allergy, Asthma and Immunology. 2012; 11(2): 133-145\u003c\/li\u003e\n        \u003cli\u003eJianHui Xiao,et al.Evidence-Based Complementary and Alternative Medicine.2012;ArticleID 273435, 15 pages\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eDocument\u003c\/h2\u003e\n    \u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/7211011_7211012_BioSci_Mouse_Lymphocyte_Separation_Medium.pdf?v=1700036452\" class=\"bf-download-card\" target=\"_blank\"\u003e\n        \u003cdiv class=\"bf-download-icon\"\u003e📄\u003c\/div\u003e\n        \u003cdiv class=\"bf-download-text\"\u003e\n            \u003ch4\u003eMouse Lymphocyte Separation Medium\u003c\/h4\u003e\n            \u003cp\u003eClick to download the PDF technical document (Instruction for Use)\u003c\/p\u003e\n        \u003c\/div\u003e\n    \u003c\/a\u003e\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eRelated Product Options:\u003c\/strong\u003e\n\u003cp\u003eSelect the appropriate format for your PBMC isolation workflow. Choose from separation medium, empty tubes, or ready-to-use prefilled tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px; margin-top: 20px;\"\u003e\n\u003c!-- Empty Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eEMPTY TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003ePBMC Separation Tube (Empty)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation?variant=52452569186485\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Human Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eHuman Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/human-lymphocyte-separation-medium?variant=43275893735605\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Mouse Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eMouse Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/mouse-lymphocyte-separation-medium?variant=43275988598965\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Prefilled Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003ePREFILLED TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eLymphocyte Separation Tube (Prefilled)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/lymphocyte-separation-tube-for-human-peripheral-blood?variant=43275829674165\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"100mL\/bottle","offer_id":43275988598965,"sku":"7211011","price":140.0,"currency_code":"USD","in_stock":true},{"title":"250mL\/bottle","offer_id":43275988697269,"sku":"7211012","price":210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_7ebc277e-4ab0-4d39-8237-78ead6d1b7d2.jpg?v=1776838756"},{"product_id":"animal-tissue-cell-total-rna-extraction-kit","title":"Animal Tissue Cell Total RNA Extraction Kit-Biosci™","description":"\u003ch2\u003eProduct Description:\u003c\/h2\u003e\n\u003cp\u003eAnimal Tissue Cell Total RNA Extraction Kit employs a rapid, phenol-free and chloroform-free RNA extraction technique for swift extraction of total RNA from easily lysable animal tissue and cell. The lysis buffer in this kit rapidly lyses cell and inactivates RNase, without the need for β-mercaptoethanol from conventional kits. The unique genomic DNA removal column guarantees effective removal of gDNA residue, eliminating the need for DNase digestion and allowing direct use of RNA for tests such as RT-PCR and qPCR. If downstream experiments are highly sensitive to trace DNA contamination, additional DNase treatment can be employed.\u003c\/p\u003e\n\u003cp\u003eThis kit extracts high-purity RNA fast and easily. Generally, the operation of a single sample can be completed within 30 minutes.\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\" style=\"margin-bottom: 16px; float: none;\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eStorage and Transportation:\u003c\/h2\u003e\n\u003cp\u003eStore at 2°C - 30°C, with a shelf life of 12 months.\u003cbr\u003eTransport at room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eScope ofApplication:\u003c\/h2\u003e\n\u003cp\u003eThe product can only be used for in vitro research\u003cbr\u003eand not for diagnostic purposes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Cultured Animal Cell Total RNA: (please read the precautions before the test).\u003c\/h2\u003e\n\u003cp\u003e1. Collect ≤ 1 × 10 7 cells by centrifuging at 300 × g for 5 min or 13,000 × g for 10 s, and remove supernatant.\u003c\/p\u003e\n\u003cp\u003e2. Add 350 μL (\u0026lt; 5 × 10 6 cells) or 600 μL (5 × 10 6 - 1 × 10 7 cells) of RLT lysis buffer, mix well by pipetting up and down, shake vigorously by hand for 20 s, and add all the lysis mixture to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Centrifuge at 13,000 × g for 1 min and retain the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Ensure that all liquid is filtered after centrifugation. Increase the centrifugal force and time if necessary.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Accurately measure the volume of the filtrate with a pipette, add an equal volume of 70% ethanol and mix well by pipetting up and down.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5. Immediately transfer the mixture to the adsorption column, which is put into the collection tube, centrifuge at 13,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Do not transfer more than 700 μL per time. If more than 700 μL of mixture needs to be transferred, do it in two separate transfers and centrifuge each time.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e6. Add 700 μL of rinsing solution 1, place at room temperature for 1 min, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e7. Add 500 μL of rinsing solution 2, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e8. Repeat Step 7.\u003c\/p\u003e\n\u003cp\u003e9. Put the adsorption column back into the collection tube and centrifuge at 13,000 × g for 2 minutes to completely remove residual ethanol via spin-drying.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The presence of residual ethanol can inhibit downstream reactions.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10. Put the adsorption column into an RNase-free 1.5 mL centrifuge tube, add 30 μL - 50 μL of eluent to the center of the adsorption column membrane, allow it to stand for 1 min, and centrifuge at 12,000 × g for 1min.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The yield can be improved by preheating the eluent in a water bath at 50°C - 70°C. If the expected RNA yield exceeds 30 μg, suck the eluent back into the adsorption column and repeat Step 10; or add another 30 μL - 50 μL of eluent and repeat Step 10, and mix the two eluents.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Animal Tissue Total RNA: (please read the precautions before the test)\u003c\/h2\u003e\n\u003cp\u003e1. Samples are first electrically homogenized or ground with liquid nitrogen.\u003c\/p\u003e\n\u003cp\u003e     a. \u003cstrong\u003eElectric homogenization:\u003c\/strong\u003e Quickly cut fresh tissues into small pieces with a scalpel, add 350 μL (\u0026lt; 20 mg of tissue) or 600 μL (20 mg - 30 mg of tissue) of RLT lysis buffer, and then electrically homogenize the mixture thoroughly for 20 - 40 s.\u003c\/p\u003e\n\u003cp\u003e     b. \u003cstrong\u003eGrinding with liquid nitrogen:\u003c\/strong\u003e Grind the tissue into fine powder with      liquid nitrogen, transfer an appropriate amount of fine tissue powder (20 mg\/30 mg) into an RNase-free 1.5 mL centrifuge tube containing 350 μL\/600 μL of RLT lysis buffer, and shake the tube vigorously by hand for 20 s to fully lyse.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOptional steps:\u003c\/strong\u003e Use a disposable 1 mL (with a 0.9 mm needle) syringe with a blunt needle to vigorously push and pull the plunger at least 10 times until a satisfactory homogenization effect is obtained, so as to shear DNAand reduce viscosity to prevent column plugging and improve yield.\u003c\/p\u003e\n\u003cp\u003e2. Centrifuge the homogenized mixture at 13,000 × g for 3 min, and transfer all the supernatant to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Follow Step 3 of extraction of cultured animal cell total RNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperation Steps:\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" data-mce-style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003ePrecautions:\u003c\/h2\u003e\n\u003cp\u003e1. Please read the operating instructions carefully before use.\u003c\/p\u003e\n\u003cp\u003e2. Before the first use, add absolute ethanol to the rinsing solution 2 reagent bottle (formal pack: 42 mL; trial pack: 4.2 mL); add absolute ethanol to the 70% ethanol bottle (formal pack: 21 mL; trial pack: 2.1 mL).\u003c\/p\u003e\n\u003cp\u003e3. Ensure that all solutions are clear before use. Low-temperature storage can cause precipitation and affect the use effect. The solution can be restored to clarity in a water bath at 37°C for several minutes.\u003c\/p\u003e\n\u003cp\u003e4. Perform all centrifugation at room temperature.\u003c\/p\u003e\n\u003cp\u003e5. The sample processing volume should not exceed the processing capacity of the genomic DNA removal column and RNA adsorption column to avoid DNA residue or yield reduction. The RNA\/DNA of various samples differs greatly, leading to a variation in the sample processing volume. When the experiment conditions are preliminarily explored, limit cell samples to 3 - 4 × 10 6 and tissue samples to 10 mg. Determine the processing volume for subsequent experiments based on the results.\u003c\/p\u003e\n\u003cp\u003e6. If residues are caused by excessive DNA content or rigorous fluorescent quantitative PCR is required, it is recommended to select intron-spanning primers or treat the RNAextract with RNase-free DNase.\u003c\/p\u003e\n\u003cp\u003e7. For the samples with excessively high DNAcontent, Step 6 can be performed as follows:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ea) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 30 s, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003eb) Add an appropriate amount of DNase working solution to the center of the adsorption column and place it at room temperature for 15 min.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ec) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 1 min, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e8. Ensure that equipment used in the experiment is RNase-free, including gloves, pipette tips, EP tubes and mortars.\u003c\/p\u003e\n\u003cp\u003e9. Bake the glassware at 150°C for 4 hours. Soak the plasticware in 0.5 M NaOH for 10 min, wash it thoroughly with water, and sterilize it to remove RNase.\u003c\/p\u003e\n\u003cp\u003e10. The product is only intended for scientific research conducted by professionals. For your safety and health, please wear lab coats and disposable gloves during the operation.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8034111_803411T_BioSci_Animal_Tissue_Cell_Total_RNA_Extraction_Kit.pdf?v=1700038025\" type=\"application\/pdf\" target=\"_blank\"\u003eAnimal Tissue Cell Total RNA Extraction Kit\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"50preps\/Kit","offer_id":43541462057141,"sku":"8034111","price":258.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8034111BioSci_AnimalTissueCellTotalRNAExtractionKit_1.png?v=1732865877"},{"product_id":"new-flash-protein-anykd-page-kit","title":"New Flash Protein AnyKD PAGE Kit-Biosci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eBioSci™ NewFlash Protein AnyKD PAGE kit is a product with a low acrylamide concentration, and adopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation rate. The kit is easy to use, and prepares gel quickly and safely.\u003c\/p\u003e\n\u003cp\u003eProtein gel preparation is simple, and can be completed in 25 min by mixing resolving gels and stacking gels at the same time.\u003c\/p\u003e\n\u003cp\u003eProtein gel electrophoresis can be completed either at 300V constant high voltage in about 25 min or at normal voltage.\u003c\/p\u003e\n\u003cp\u003eThe kit is suitable for separation and identification of 10-250 kDa protein, and there is no need to adjust the concentration of resolving gel according to protein molecular weight.\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 72.6632%; height: 108px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 72.6632%; height: 108px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eStacking Gel SolutionA\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eStacking Gel Solution B\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eResolving Gel SolutionA\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e125 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eResolving Gel Solution B\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e125 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eAmmonium Persulfate(APS)\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e0.5 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eThe number of gels prepared per kit\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\" data-mce-style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e0.75mm Mini-gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e1.0mm Mini-gel \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e1.5mm Mini-gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e62 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e50 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e33 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eIt will keep for 12 months if stored away from light at 4℃.\u003c\/p\u003e\n\u003cp\u003eAdd 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS solution, and repack the solution into small portions for storage.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplicability\u003c\/h2\u003e\n\u003cp\u003eFor in vitro study only. Not for use as a diagnostic reagent.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cp\u003e1. This product contains a small amount of acrylamide which is corrosive. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/p\u003e\n\u003cp\u003e2. This product is only used for scientific research by professionals and shall neither be used for clinical diagnosis, treatment, food or drugs nor stored in ordinary housing.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperating Instructions\u003c\/h2\u003e\n\u003cp\u003e1. With BioSci™ NewFlash Protein AnyKD PAGE kit, uniformly mix resolving gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 2.0\/2.5\/3.8 mL of resolving gel solutionsAand B). Gel preparation can be completed in a 15 mL or 50 mL centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e2. With BioSci™ NewFlash Protein AnyKD PAGE kit, uniformly mix stacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 0.8\/1.0\/1.5 mL of stacking gel solutionsAand B).\u003cbr\u003e\u003cstrong\u003eNote: With this product, gelation is rapid. Do not wait for pouring the resolving gel into the glass pates to start the preparation of stacking gel. Excessive waiting time will lead to partial polymerization of the resolving gel, resulting in uneven division surface of resolving gel and stacking gel, which will influence the electrophoresis effect.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e3. Add 10% APS solution to the resolving gel solution in step 1 (for 5 mL of resolving gel solution, add 50 µL of 10% APS solution), and pour the uniformly mixed resolving gel solution into the glass pates to a position 1.5 cm from the top of the front glass plate or 0.5 cm from teeth of a comb.\u003cbr\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Add 10% APS solution to the stacking gel solution in step 2 (for 2 mL of stacking gel solution, add 20 µL of 10% APS solution), and directly pour the uniformly mixed stacking gel solution on the resolving gel solution without waiting for solidification of the resolving gel solution.\u003cbr\u003e\u003cstrong\u003eNote: The kit can complete protein gel casting at one time without mixing stacking gel with resolving gel.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization.\u003cbr\u003e\u003cstrong\u003eNote: Gels can be stored for several weeks at 4℃ in sealing bags with a small amount of electrophoresis buffer.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette for sample loading. It is recommended that BioSci™ ColorBand Prestained Protein Marker (Cat.No.8011021\u0026amp;8011031) shall be used to indicate the molecular weight of the protein samples on PAGE gel.\u003c\/p\u003e\n7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis buffer.\u003cbr\u003eThe voltage can be set to a maximum of 300 V, at which electrophoresis can be completed in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if the current is too high (to avoid much heat).\n\u003cp\u003e\u003cstrong\u003eNote: The electrophoresis of this product can be carried out at normal voltage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"Default Title","offer_id":43348931182773,"sku":"8012011","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012011BioSci_NewFlashProteinAnyKDPAGEKit_1.png?v=1732878254"},{"product_id":"instant-pbs-buffer-biosci","title":"Instant PBS Buffer-SuperCulture™","description":"\u003ch2\u003eProduct Composition\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 71.0995%; height: 46px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003e8005011\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 18px;\"\u003e8005012\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003eInstant PBS Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003e20 packs\/box\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 28px;\"\u003e100 packs\/box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cstrong\u003eNote: The pH value of 1 × solution is 7.4±0.2@25°C.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eStorage and Transportation Conditions\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature of 10-30°C, protected from light, with a shelf life of 36 months.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eInstant PBS Buffer is white to off-white instant granules, and each pack of this product can produce 1 L of 1 × PBS Buffer, which is easy to operate and use. The main components of PBS are sodium chloride, potassium chloride and phosphate. The concentration of sodium chloride, potassium chloride and phosphate in 1 × PBS Buffer is 137 mM, 2.7 mM and 10 mM respectively.\u003c\/p\u003e\n\u003cp\u003eInstant PBS Buffer is widely used in biological research, which can be used in immunoassay tests, microbiology experiments, protein biochemical experiments, etc. The Instant PBS Buffer prepared with PBS instant granules can be used for cell culture, washing and other related experiments after being treated with 0.22 μm sterilizing filter.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:All relevant raw materials areAR\/GR grade.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eProcedure\u003c\/h2\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant PBS Buffer.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar until it is completely dissolved. (This step can also be performed with magnetic stirrer)\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Transfer the PBS solution in Step 2 into a constant volume container, and add water to dilute to 1 L, which is 1 × working solution.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eNote: The commonly used working concentration of Instant PBS Buffer is 0.5 ×, and in this case, 1 × working solution should be diluted before use.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-right: auto; margin-left: auto; float: none; display: block;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206092157_1024x1024.png?v=1701825751\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eNote: Wash the container sufficiently with pure water before preparation, and clean it in time after preparation.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8005011_8005012_SuperDissol_Instant_PBS_Buffer.pdf?v=1701828220\" type=\"application\/pdf\" target=\"_blank\"\u003eInstant PBS Buffer-Biosci\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003esuperculture\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"20 packs\/box","offer_id":43380245135541,"sku":"8005011","price":44.0,"currency_code":"USD","in_stock":true},{"title":"100 packs\/box","offer_id":43380245168309,"sku":"8005012","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/80050118005012SuperDissol_InstantPBSBuffer_1.png?v=1732875250"},{"product_id":"instant-tbs-t-buffer-biosci","title":"Instant TBS-T Buffer-SuperCulture™","description":"\u003ch2\u003eProduct Composition\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 71.0995%; height: 46px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003e8015131\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 18px;\"\u003e8015132\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003eInstant TBS-T Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003e20 packs\/box\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 28px;\"\u003e100 packs\/box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The pH value of 1 × solution is 7.4±0.2@25°C.\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage and Transportation Conditions\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature of 10-30°C, protected from light, with a shelf life of 36 months.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eInstant TBS-T Buffer is white to off-white instant granules, and each pack of this product can produce 1 L of 1 × TBS-T Buffer, which is easy to operate and use. The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salt and Tween-20. The concentration of sodium chloride, potassium chloride and Tris buffer salt in 1 × TBS-T Buffer is 137 mM, 2.7 mM and 25 mM respectively, and the content of Tween-20 is 0.05%.\u003c\/p\u003e\n\u003cp\u003eInstant TBS-T Buffer is widely used in biology, which is mainly used in immunohistochemistry, in situ hybridization, ELISA and western blot, and is also commonly used as wash buffer, as well as for antibody dilution and preparation of blocking buffer.\u003c\/p\u003e\n\u003ch2\u003eProcedure\u003c\/h2\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant TBS-T Buffer.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar until it is completely dissolved. (This step can also be performed with magnetic stirrer)\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Transfer the TBS-T solution in Step 2 into a constant volume container, and add water to dilute to 1 L, which is 1 × working solution.\u003c\/p\u003e\n\u003ch2\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206101453_1024x1024.png?v=1701828943\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eNote: Wash the container sufficiently with pure water before preparation, and clean it in time after preparation.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015131_8015132_TBS-T_SuperDissol_Instant_TBS-T_Buffer.pdf?v=1701828598\" type=\"application\/pdf\" target=\"_blank\"\u003eInstant TBS-T Buffer-Biosci\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003esuperculture\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"20 packs\/box","offer_id":43380281475253,"sku":"8015131","price":65.0,"currency_code":"USD","in_stock":true},{"title":"100 packs\/box","offer_id":43380281508021,"sku":"8015132","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/80151318015132TBS-TSuperDissol_InstantTBS-TBuffer_1.png?v=1732875273"},{"product_id":"instant-tbs-t-blocking-buffer-biosci","title":"Instant TBS-T Blocking Buffer-SuperDissol™","description":"\u003ch2\u003eProduct Composition\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 71.0995%; height: 46px;\" data-mce-style=\"border-collapse: collapse; width: 71.0995%; height: 46px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\" data-mce-style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\" data-mce-style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003e8015151\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 18px;\" data-mce-style=\"width: 33.0915%; text-align: center; height: 18px;\"\u003e8015152\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\" data-mce-style=\"height: 28px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\" data-mce-style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003eInstant TBS-T\u003cbr\u003eBlocking Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\" data-mce-style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003e20 packs\/box\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 28px;\" data-mce-style=\"width: 33.0915%; text-align: center; height: 28px;\"\u003e100 packs\/box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003cstrong\u003eNote: The pH value of 1 × solution is 7.4±0.2@25°C.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage and Transportation Conditions\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature of 10-30°C, protected from light, with a shelf life of 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eInstant TBS-T Blocking Buffer is off-white to light yellow granules, and each pack of this product can produce 100 ml TBS-T Blocking Buffer, which is easy to operate. Its main component is the selected fish gelatin protein, and the content of protein is 5% after being prepared into 1 × working solution. It is used for antibody blocking steps of Western Blot and ELISA, and provides the TBS-T Blocking Buffer containing the detergent Tween 20.\u003cbr\u003eThe fish gelatin used in Instant TBS-T Blocking Buffer is compatible with most proteins, providing excellent reactivity and compatibility, which can complete the blocking within 15 minutes, shortening the user's experimental time. It has been premixed with TBS-T for easy use, and the presence of detergent Tween 20 improves the blocking efficiency in many Western Blot detection reactions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProcedure\u003c\/h2\u003e\n\u003cstrong\u003ePreparation Steps\u003c\/strong\u003e\n\u003cp\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant TBS-T Blocking Buffer.\u003c\/p\u003e\n\u003cp\u003e2. Measure about 50 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar until it is completely dissolved. (This step can also be performed with magnetic stirrer)\u003c\/p\u003e\n\u003cp\u003e3. Transfer the Instant TBS-T Blocking Buffer in Step 2 into a constant volume container, and add water to dilute to 100 ml, which is 1 × working solution.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eBlocking Steps\u003c\/h2\u003e\n\u003cp\u003e1. After the transfer is completed, put the transfer membrane into the hybrid incubation box, add 10~20 ml of 1 × TBS-T Blocking Buffer, cover the surface of the carrier, and incubate it at room temperature for about 10 min on a desktop horizontal shaker;\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: The blocking effect of this product for 10 min is significantly better than that of conventional BSA for 1 h. For antibodies with higher background, the blocking time can be extended to 30~60 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e2. The blocking membrane can be used for subsequent experiments such as primary antibody incubation.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015151_8015152_SuperDissol_Rapid_Blocking_Buffer.pdf?v=1701842823\" type=\"application\/pdf\" target=\"_blank\"\u003eSuperDissol™ Instant TBS-T Blocking Buffer-Biosci\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"20 packs\/box","offer_id":43380921106613,"sku":"8015151","price":103.0,"currency_code":"USD","in_stock":true},{"title":"100 packs\/box","offer_id":43380921139381,"sku":"8015152","price":464.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8015151SuperDissol_RapidBlockingBuffer.png?v=1732875261"},{"product_id":"superculture-tm-l500-serum-free-medium-for-lymphocyte-gmp","title":"Biofargo L500 Serum-Free Medium for Lymphocyte (GMP)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture™ L500 serum-free medium for lymphocyte is an upgraded medium developed by Dakewe based on the SuperCulture TM L100 serum-free medium for lymphocyte and applicable to in vitro culture of human lymphocytes. This product is a chemically defined medium, all the compositions of which are produced from cell culture grade materials. This product contains no heterogeneous animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research and achieve the high-density culture of immunocytes such as human lymphocytes, cytokine induced killer cells, natural killer cells and dendritic cells.\u003c\/p\u003e\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture TM L500 serum-free medium for lymphocyte supports the high-density suspension culture of lymphocytes. It supports the suspension culture of lymphocytes with the density of 5×10 5 ~5×10 6 cells\/mL in a static culture container. To ensure sufficient gas exchange of the culture system, the recommended depth of the medium is 1~1.5 cm. The medium also supports the high-density culture of lymphocytes in a bioreactor, and the optimization of the culture procedure should be determined by researchers based on experience.\u003c\/p\u003e\n\u003cp\u003eThe SuperCulture TM L500 serum-free medium for lymphocyte supports the serum-free culture of immunocytes, and the addition of autologous plasma, human AB serum, FBS or serum substitutes can significantly improve the cell viability and increase the cell proliferation rate. The SuperGrow TM cell culture supplemental mix is recommended to obtain the optimal culture result.\u003c\/p\u003e\n\u003ch2\u003eExample: Multiplication Culture of Cytokine Induced Killer Cells\u003c\/h2\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Place the SuperCulture TM L500 serum-free medium for lymphocyte at room temperature for equilibrium.The following operations shall be in an aseptic condition:\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Separate lymphocytes of human peripheral blood in an aseptic condition.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Inoculate PBMCs obtained by separation to a T75 or T175 cell culture bottle with the SuperCulture TM L500 serum-free medium for lymphocyte at a concentration of 2~2.5×10 6 cells\/mL, add recombinant human IFN-γ, etc. , and culture in a 5% CO 2 incubator at 37℃.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. After 24 hr, i.e., on the first day, add CD3 monoclonal antibody, recombinant human IL-2, etc. to stimulate growth and proliferation of cytokine induced killer cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. From the fourth day, sample every two days to calculate the cell concentration, supplement fresh SuperCulture TM L500 serum-free medium for lymphocyte based on the calculation result, and adjust the cell concentration to 1.5×10 6 cells\/mL.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e6. On the seventh day, change a bottle with a larger volume or transfer into a cell culture bag according to the volume of the cell culture suspension. The maximum culture volume of a T75 culture bottle is 40 mL, and the maximum culture volume of a T175 culture bottle is 200 mL. When the volume of the medium is more than 200 mL, transfer the cell culture suspension into a cell culture bag.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e7. Cell proliferation and cell surface marker detection.\u003c\/p\u003e\n\u003ch2\u003ePreservation Method\u003c\/h2\u003e\n\u003cp\u003eKeep away from light at 2-8℃ , valid for 12 months.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cp\u003ePay attention to operate in an aseptic condition.\u003cbr\u003eThis product is only for research.\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display: none;\"\u003esuperculture\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"1000mL\/Bottle","offer_id":43540989477045,"sku":"6811021","price":212.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6111021L500.png?v=1732875849"},{"product_id":"supercut™-xhoⅰ-biosci","title":"XhoI-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…C↓T C G A G…3'\u003cbr\u003e3'…G A G C T↑C…5'\u003cbr\u003eIsoschizomers*: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231206151502_160x160.png?v=1701846927\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ XhoⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™XhoⅠ.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ AvrII was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ XhoⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria- Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be\u003cbr\u003eexpressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eTo 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003eTo 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eTo 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently suck or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃ or add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eimpaired\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"5000 units","offer_id":43541335736501,"sku":"8052441","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052441SuperCut_XhoIFastRestrictionEndonucleasespng.png?v=1732886496"},{"product_id":"supercut™-ndeⅰ-biosci","title":"NdeⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...C A↓T A T G...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...G T A T ↑A C...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: FauNDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206161534_160x160.png?v=1701850606\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NdeⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ NdeⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ NdeⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NdeⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NdeⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NdeⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NdeⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™  NdeⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"2000 units","offer_id":43541352087733,"sku":"8052251","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_91317f02-dc20-4d8e-823b-55878e93d954.jpg?v=1753176201"},{"product_id":"supercut™-nsiⅰ","title":"NsiⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...A T G C A↓T...3'\u003cbr\u003e3'...T↑A C G T A...5'\u003cbr\u003eIsoschizomers*: EcoT22I, Mph1103I, Zsp2IDI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206165333_160x160.png?v=1701852836\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ NsiⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 1 μL of SuperCut™ NsiⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NsiⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NsiⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NsiⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NsiⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e14\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e9\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500 units","offer_id":43541364605109,"sku":"8052291","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052291SuperCut_NsiIpng.png?v=1732878695"},{"product_id":"supercut™-hindⅲ-biosci","title":"HindⅢ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…A↓A G C T T…3'\u003cbr\u003e3'…T T C G A↑A…5'\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206170842_160x160.png?v=1701853741\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ HindⅢ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ HindⅢ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ HindⅢ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ HindⅢ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ HindⅢ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ HindⅢ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e12\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eSome impaired\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"10000 units","offer_id":43541383184565,"sku":"8052181","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052181SuperCut_Hind_FastRestrictionEndonucleasespng.png?v=1732874002"},{"product_id":"dulbecco-s-modified-eagle-s-medium-low-glucose-biosci™","title":"Biofargo Dulbecco's Modified Eagle's Medium-High Glucose-No HEPES","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eDulbecco's Modified Eagle's Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg\/L glucose, 1 mM sodium pyruvate, and 1500 mg\/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eContains 4 mM L-glutamine, 4500 mg\/L glucose, 1 mM sodium pyruvate, and 1500 mg\/L sodium bicarbonate. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNOTE:\u003c\/strong\u003e\u003cspan\u003e This reduced level of sodium bicarbonate (NaHCO\u003c\/span\u003e\u003csub\u003e3\u003c\/sub\u003e\u003cspan\u003e, 1.5 g\/L) is intended for use in 5% CO\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003e in air.  Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cp\u003eThe medium is produced by aseptic processes and can be used for further manufacturing purposes. This product is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStored at 2℃~8℃, away from light, the shelf life is 12 months.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cp\u003eAvoid repeated freezing and thawing.\u003cbr\u003ePay attention to operating in an aseptic condition.\u003c\/p\u003e","brand":"Biofargo","offers":[{"title":"500mL\/Bottle","offer_id":43395034022069,"sku":"6016112","price":16.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/E600002.jpg?v=1732870938"},{"product_id":"rpmi-1640-medium-biosci™","title":"Biofargo RPMI-1640 Medium","description":"\u003cp\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n  \/* =========================\n     Biofargo RPMI Product Page\n     Scoped styles only\n     ========================== *\/\n  .biofargo-wrap,\n  .biofargo-wrap *,\n  .biofargo-wrap *::before,\n  .biofargo-wrap *::after {\n    box-sizing: border-box;\n  }\n\n  .biofargo-wrap .biofargo-table-wrap {\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n    border: 1px solid #eee;\n    border-radius: 10px;\n    padding: 6px;\n    background: #fff;\n    margin-top: 15px;\n  }\n\n  .biofargo-wrap table.biofargo-table {\n    width: 100%;\n    border-collapse: collapse;\n    font-size: 14px;\n    min-width: 640px;\n    background: #fff;\n  }\n\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td {\n    border: 1px solid #e9ecef;\n    padding: 15px;\n    vertical-align: middle;\n    white-space: normal;\n    word-break: break-word;\n  }\n\n  .biofargo-wrap table.biofargo-table thead th {\n    background: #f2f2f2;\n    color: #000;\n    font-weight: 700;\n    text-align: left;\n  }\n\n  .biofargo-wrap table.biofargo-table tbody td:first-child {\n    background: #f8f9fa;\n    font-weight: 600;\n    width: 30%;\n  }\n\n  .biofargo-wrap .biofargo-formulation-table tbody td:first-child {\n    width: 70%;\n  }\n\n  .biofargo-wrap .biofargo-section {\n    padding: 30px;\n    border-bottom: 8px solid #f4f6f8;\n  }\n\n  .biofargo-wrap .biofargo-section:last-child {\n    border-bottom: 0;\n  }\n\n  .biofargo-wrap .biofargo-heading {\n    font-size: 22px;\n    color: #0056b3;\n    margin: 0 0 20px;\n  }\n\n  .biofargo-wrap .biofargo-subheading {\n    font-size: 18px;\n    color: #0056b3;\n    margin: 25px 0 12px;\n  }\n\n  .biofargo-wrap .biofargo-section p {\n    margin: 0 0 14px;\n  }\n\n  .biofargo-wrap .biofargo-section ul {\n    margin: 0;\n    padding-left: 22px;\n  }\n\n  .biofargo-wrap .biofargo-section li {\n    margin-bottom: 8px;\n  }\n\n  .biofargo-wrap .biofargo-faq details {\n    margin-bottom: 15px;\n    font-size: 15px;\n    line-height: 1.6;\n  }\n\n  .biofargo-wrap .biofargo-faq summary {\n    cursor: pointer;\n    font-weight: 600;\n  }\n\n  .biofargo-wrap .biofargo-faq details p {\n    margin: 8px 0 0;\n    padding-left: 15px;\n  }\n\n  @media (max-width: 640px) {\n    .biofargo-wrap .biofargo-section {\n      padding: 22px 18px;\n    }\n\n    .biofargo-wrap .biofargo-heading {\n      font-size: 20px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee; background: #fff;\" class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003e\n\u003cp\u003eSelecting an RPMI-1640 cell culture medium requires confirming whether the formulation contains L-glutamine, phenol red, sodium bicarbonate, HEPES, sodium pyruvate, or antibiotics. This RPMI-1640 Medium contains L-glutamine, phenol red, and sodium bicarbonate, while HEPES, sodium pyruvate, and antibiotics are not included.\u003c\/p\u003e\n\u003cp\u003eRPMI-1640 Medium was developed by Moore et al. at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. It has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas.\u003c\/p\u003e\n\u003cp\u003eThis RPMI-1640 cell culture medium requires supplementation and is commonly supplemented with 10% fetal bovine serum (FBS).\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003e\u003cstrong\u003eFormulation overview:\u003c\/strong\u003e With L-glutamine, phenol red, and sodium bicarbonate; without HEPES, sodium pyruvate, or antibiotics.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- Specifications --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eAttribute\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eRPMI-1640 Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog Number\u003c\/td\u003e\n\u003ctd\u003e6016011\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSupplementation\u003c\/td\u003e\n\u003ctd\u003eRequires supplementation; commonly supplemented with 10% fetal bovine serum (FBS)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Glutamine\u003c\/td\u003e\n\u003ctd\u003eIncluded, 300 mg\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhenol Red\u003c\/td\u003e\n\u003ctd\u003eIncluded, 5 mg\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAntibiotics\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Bicarbonate\u003c\/td\u003e\n\u003ctd\u003eIncluded, 2000 mg\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHEPES\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH\u003c\/td\u003e\n\u003ctd\u003e7.0–7.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOsmolality\u003c\/td\u003e\n\u003ctd\u003e265–305 mOsmol\/kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.3 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e2–8°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- Features --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"font-size: 15px; color: #444;\"\u003e\n\u003cli\u003eContains L-glutamine at 300 mg\/L.\u003c\/li\u003e\n\u003cli\u003eContains phenol red at 5 mg\/L.\u003c\/li\u003e\n\u003cli\u003eContains sodium bicarbonate at 2000 mg\/L.\u003c\/li\u003e\n\u003cli\u003eDoes not contain HEPES, sodium pyruvate, or antibiotics.\u003c\/li\u003e\n\u003cli\u003eSpecified pH range of 7.0–7.6.\u003c\/li\u003e\n\u003cli\u003eSpecified osmolality range of 265–305 mOsmol\/kg.\u003c\/li\u003e\n\u003cli\u003eEndotoxin specification of less than 0.3 EU\/mL.\u003c\/li\u003e\n\u003cli\u003eSupplied in a 500 mL format.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- Applications --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eApplications\u003c\/h2\u003e\n\u003cp style=\"font-size: 15px; color: #444;\"\u003eThis RPMI-1640 Medium is suitable for the mammalian cell culture applications and cell types identified in the product information:\u003c\/p\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(260px,1fr)); gap: 10px 35px;\"\u003e\n\u003cul style=\"font-size: 15px; color: #444;\"\u003e\n\u003cli\u003eSuspension culture of human leukemic cells\u003c\/li\u003e\n\u003cli\u003eMonolayer culture of human leukemic cells\u003c\/li\u003e\n\u003cli\u003eHeLa cell culture\u003c\/li\u003e\n\u003cli\u003eJurkat cell culture\u003c\/li\u003e\n\u003cli\u003eMCF-7 cell culture\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"font-size: 15px; color: #444;\"\u003e\n\u003cli\u003ePC12 cell culture\u003c\/li\u003e\n\u003cli\u003ePBMC culture\u003c\/li\u003e\n\u003cli\u003eAstrocyte culture\u003c\/li\u003e\n\u003cli\u003eHybridoma culture\u003c\/li\u003e\n\u003cli\u003eCulture of the listed mammalian cell types\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- Formulation --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eFormulation\u003c\/h2\u003e\n\u003cp style=\"font-size: 15px; color: #444;\"\u003eThe following RPMI-1640 formulation components are listed in milligrams per liter (mg\/L).\u003c\/p\u003e\n\u003ch3 class=\"biofargo-subheading\"\u003eAmino Acids\u003c\/h3\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table biofargo-formulation-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eConcentration (mg\/L)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlycine\u003c\/td\u003e\n\u003ctd\u003e10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Arginine (free base)\u003c\/td\u003e\n\u003ctd\u003e200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Asparagine (anhyd.)\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Aspartic Acid\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Cystine·2HCl\u003c\/td\u003e\n\u003ctd\u003e65\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Glutamic Acid\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Glutamine\u003c\/td\u003e\n\u003ctd\u003e300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Histidine (free base)\u003c\/td\u003e\n\u003ctd\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Hydroxyproline\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Isoleucine\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Leucine\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Lysine·HCl\u003c\/td\u003e\n\u003ctd\u003e40\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Methionine\u003c\/td\u003e\n\u003ctd\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Phenylalanine\u003c\/td\u003e\n\u003ctd\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Proline\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Serine\u003c\/td\u003e\n\u003ctd\u003e30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Threonine\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Tryptophan\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Tyrosine·2Na·2H₂O\u003c\/td\u003e\n\u003ctd\u003e29\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Valine\u003c\/td\u003e\n\u003ctd\u003e20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"biofargo-subheading\"\u003eVitamins\u003c\/h3\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table biofargo-formulation-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eConcentration (mg\/L)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eD-Biotin\u003c\/td\u003e\n\u003ctd\u003e0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCholine Chloride\u003c\/td\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFolic Acid\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003emyo-Inositol\u003c\/td\u003e\n\u003ctd\u003e35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNiacinamide\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ep-Amino Benzoic Acid\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD-Pantothenic Acid·1\/2 Ca\u003c\/td\u003e\n\u003ctd\u003e0.25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePyridoxal·HCl\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRiboflavin\u003c\/td\u003e\n\u003ctd\u003e0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThiamine·HCl\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVitamin B-12\u003c\/td\u003e\n\u003ctd\u003e0.005\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"biofargo-subheading\"\u003eInorganic Salts\u003c\/h3\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table biofargo-formulation-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eConcentration (mg\/L)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eCa(NO₃)₂·4H₂O\u003c\/td\u003e\n\u003ctd\u003e100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMgSO₄ (anhyd.)\u003c\/td\u003e\n\u003ctd\u003e48.84\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKCl\u003c\/td\u003e\n\u003ctd\u003e400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNaHCO₃\u003c\/td\u003e\n\u003ctd\u003e2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNaCl\u003c\/td\u003e\n\u003ctd\u003e6000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNaH₂PO₄ (anhyd.)\u003c\/td\u003e\n\u003ctd\u003e800\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"biofargo-subheading\"\u003eOther Components\u003c\/h3\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table biofargo-formulation-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eComponent\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eConcentration (mg\/L)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eD-Glucose\u003c\/td\u003e\n\u003ctd\u003e2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlutathione (reduced)\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhenol Red·Na\u003c\/td\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- Storage and Handling --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eStorage and Handling\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 25px;\"\u003e\n\u003cdiv style=\"flex: 1; min-width: 260px;\"\u003e\n\u003ch3 style=\"font-size: 18px; color: #0056b3; margin: 0 0 12px;\"\u003eStorage\u003c\/h3\u003e\n\u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background: #fff5f5; color: #c53030; font-size: 16px; border-radius: 4px;\"\u003e\u003cstrong\u003eStore at 2–8°C and protect from light.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; margin-top: 12px;\"\u003eThe stated shelf life is 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex: 1; min-width: 260px;\"\u003e\n\u003ch3 style=\"font-size: 18px; color: #0056b3; margin: 0 0 12px;\"\u003eHandling Notes\u003c\/h3\u003e\n\u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background: #fff; font-size: 14px;\"\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003eUse aseptic technique during handling.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c!-- FAQ --\u003e\n\u003csection class=\"biofargo-section biofargo-faq\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this RPMI-1640 Medium contain L-glutamine?\u003c\/summary\u003e\n\u003cp\u003eYes. This RPMI-1640 formulation contains L-glutamine at 300 mg\/L.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this RPMI-1640 Medium contain phenol red?\u003c\/summary\u003e\n\u003cp\u003eYes. The formulation contains phenol red sodium salt at 5 mg\/L.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this RPMI-1640 Medium contain sodium bicarbonate?\u003c\/summary\u003e\n\u003cp\u003eYes. The formulation contains sodium bicarbonate at 2000 mg\/L.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this RPMI-1640 formulation contain HEPES?\u003c\/summary\u003e\n\u003cp\u003eNo. HEPES is not included in this RPMI-1640 Medium.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the medium contain sodium pyruvate or antibiotics?\u003c\/summary\u003e\n\u003cp\u003eNo. Sodium pyruvate and antibiotics are not included in this formulation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RPMI-1640 Medium require FBS supplementation?\u003c\/summary\u003e\n\u003cp\u003eThe product information states that RPMI-1640 Medium requires supplementation and is commonly supplemented with 10% fetal bovine serum (FBS).\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhich cells are listed as suitable for this RPMI-1640 Medium?\u003c\/summary\u003e\n\u003cp\u003eThe product information lists HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this RPMI-1640 Medium suitable for suspension cell culture?\u003c\/summary\u003e\n\u003cp\u003eThe product information states that RPMI-1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the specified pH and osmolality ranges?\u003c\/summary\u003e\n\u003cp\u003eThe specified pH range is 7.0–7.6, and the specified osmolality range is 265–305 mOsmol\/kg.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the endotoxin specification?\u003c\/summary\u003e\n\u003cp\u003eThe stated endotoxin specification is less than 0.3 EU\/mL.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow should RPMI-1640 Medium be stored?\u003c\/summary\u003e\n\u003cp\u003eStore the medium at 2–8°C and protect it from light. The stated shelf life is 12 months.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- Documents --\u003e\n\u003csection class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-heading\"\u003eDocuments\u003c\/h2\u003e\n\u003cp style=\"font-size: 15px; margin-bottom: 0;\"\u003e\u003ca rel=\"noopener noreferrer\" type=\"application\/pdf\" href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/RPMI-1640_Medium.pdf?v=1702434025\" target=\"_blank\"\u003e \u003cstrong\u003eRPMI-1640 Medium Product Datasheet (PDF)\u003c\/strong\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c!-- Product and FAQ Structured Data --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Biofargo","offers":[{"title":"500mL\/Bottle","offer_id":43398631719093,"sku":"6016011","price":20.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_dac8bca8-dcdf-4f36-bd97-48f066036382.jpg?v=1785219299"},{"product_id":"natural-killer-cells-induction-culture-kit-2-0-biosci™","title":"Natural Killer Cells Induction Culture Kit 2.0-Biosci™","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture™ Natural Killer Cells Induction Culture Kit 2.0 is a culture kit for in vitro culture of NK cells developed in GMP confirming processes by Dakewe, which contains N500 Serum Free Medium for NK Cells, NK cell activator and NK cell stimulator. This product is a chemically defined, all the compositions of which are produced from medicinal\/cell culture grade materials. This product contains no animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research. The optimized formula of this product can significantly improve the proportion, the viability and the killing rate of NK cells in cells cultured in vitro.\u003c\/p\u003e\n\u003ch2\u003ePacking Specification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 82.8258%; height: 64px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.6694%; height: 18px; text-align: center;\"\u003eCat.\u003c\/td\u003e\n\u003ctd style=\"width: 19.3068%; height: 18px; text-align: center;\"\u003eSpecification\u003c\/td\u003e\n\u003ctd style=\"width: 43.5427%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 19.6694%; height: 10px; text-align: center;\"\u003e6813531\u003c\/td\u003e\n\u003ctd style=\"width: 19.3068%; height: 10px; text-align: center;\"\u003e3L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 43.5427%; height: 10px; text-align: left;\"\u003e\n\u003cp\u003e  NK cell stimulator                   150μL\u003c\/p\u003e\n\u003cp\u003e  NK cell activator                     500μL×3\u003c\/p\u003e\n\u003cp\u003e  N500 Serum Free Medium       1L×3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.6694%; height: 18px; text-align: center;\"\u003e6813532\u003c\/td\u003e\n\u003ctd style=\"width: 19.3068%; height: 18px; text-align: center;\"\u003e2L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 43.5427%; height: 18px;\"\u003e\n\u003cp\u003e  NK cell stimulator                   100μL\u003c\/p\u003e\n\u003cp\u003e  NK cell activator                     500μL×2\u003c\/p\u003e\n\u003cp\u003e  N500 Serum Free Medium       1L×2 \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.6694%; height: 18px; text-align: center;\"\u003e6813533\u003c\/td\u003e\n\u003ctd style=\"width: 19.3068%; height: 18px; text-align: center;\"\u003e1L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 43.5427%; height: 18px;\"\u003e\n\u003cp\u003e  NK cell stimulator                   50μL\u003c\/p\u003e\n\u003cp\u003e  NK cell activator                     500μL\u003c\/p\u003e\n\u003cp\u003e  N500 Serum Free Medium       1L\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture TM Natural Killer Cells Induction Culture Kit 2.0 supports the high-density suspension culture of NK cells. In a static culture container, to ensure good gas exchange of the culture system, the recommended depth of the medium is not more than 1.5 cm. The medium supports the high-density culture of NK cells in a bioreactor, and \u003cstrong\u003ethe optimization of the culture procedure is determined by users based on experience.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe SuperCulture TM Natural Killer Cells Induction Culture Kit 2.0 supports the serum free culture of NK cells, and the addition of autologous plasma, human AB serum, FBS or serum substitutes can significantly improve the cell state and increase the cell proliferation rate. \u003cspan style=\"text-decoration: underline;\"\u003eAutologous plasma or SuperGrow TM cell culture additive (Cat#: 6122011\/6122012) is recommended to obtain the optimal culture effect.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eCulture Example\u003c\/h2\u003e\n\u003cp\u003e1. lace the SuperCulture TM N500 serum free medium for NK cells at room temperature for equilibrium.\u003c\/p\u003e\n\u003cp\u003eThe following operations shall be in an aseptic condition:\u003c\/p\u003e\n\u003cp\u003e2. Add 500 uL of NK cell activator per liter of SuperCulture TM N500 serum free medium for NK cells to prepare an amplification medium for NK cells.\u003c\/p\u003e\n\u003cp\u003e3. Separate lymphocytes of human peripheral blood in an aseptic condition (it is recommended to use a Biosci™ human lymphocyte separation tube\/separation medium, Cat#: 7912011\/7922021).\u003c\/p\u003e\n\u003cp\u003e4. Heat-inactivated autologous plasma: obtain plasma by separation, heat-treat the plasma at 56℃ for 30 min, centrifuge the plasma at 1000 g for 10 min, and then extract the supernatant to obtain heat-inactivated autologous plasma.\u003c\/p\u003e\n\u003cp\u003e5. On the zeroth day, inoculate PBMCs obtained by separation to a T75 or T175 cell culture bottle with the amplification medium for NK cells (containing 10% of heat-inactivated autologous plasma) at a concentration of 2.5~3.0×10 6 cells\/mL, add a NK cell stimulator (100×) , and culture in a 5% CO₂ incubator at 37℃.\u003c\/p\u003e\n\u003cp\u003e6. On the third day, supplement fresh amplification medium for NK cells (containing 10% of heat-inactivated autologous plasma). The volume ratio of the original medium to the supplemented fresh amplification medium for NK cells is 1:2. (For example, for 10 mL of original medium, supplement 20 mL of fresh amplification medium for NK cells.)\u003c\/p\u003e\n\u003cp\u003e7. From the fifth day, sample every two days to calculate the cell concentration, supplement fresh amplification medium for NK cells (containing 5% of heat-inactivated autologous plasma) based on the calculation result, and adjust the cell concentration to 0.8~1×10 6 cells\/mL. (The cell density can be adjusted according to changes of medium color. When cells are passaged, the passage density can be adjusted to 0.8~0.9×10 6 cells\/mL if the medium is yellow, and can be adjusted to 1×10 6 cells\/mL if the medium is red.)\u003c\/p\u003e\n\u003cp\u003e8. On the seventh day, change a bottle with a larger volume or transfer into a cell culture bag (it is recommended to use a Biosci™ cell culture bag, Cat#: 6071011\/6071012) according to the volume of the cellculture suspension. The maximum culture volume of a T75 culture bottle is 40 mL, and the maximum culture volume of a T175 culture bottle is 200 mL. When the volume of the medium is more than 200 mL, transfer the cell culture suspension into a cell culture bag.\u003c\/p\u003e\n\u003cp\u003e9. After the seventh day, the content of the heat-inactivated autologous plasma in the supplemented fresh amplification medium for NK cells can be reduced to 1%.\u003c\/p\u003e\n\u003cp\u003e10. Cell proliferation and cell surface marker detection.\u003c\/p\u003e\n\u003ch2\u003ePreservation Method\u003c\/h2\u003e\n\u003cp\u003eKeep NK cell stimulator and NK cell activator at -25~ -10℃, valid for 12 months.\u003cbr\u003eKeep N500 medium away from light at 2~8℃, valid for 12 months.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDuring blood collection, heparin or sodium citrate anticoagulant can be used, but EDTA anticoagulant cannot be used.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe blood collection volume can be estimated based on 1×10 6 PBMCs per milliliter of peripheral blood.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePlace the medium at room temperature for equilibrium or extract an estimated amount, and reheat to 37℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIf the temperature of the medium is too low or the cell density is too high, flocculated cells may appear, and the cell viability is reduced.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell passage shall be carried out gently to avoid mechanical damages to cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe amplification medium for NK cells is valid for three weeks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse up the NK cell stimulator and NK cell activator at one time, and it shall not be frozen and thawed repeatedly, and shall be used within the validity of the product. After the complete medium is prepared by mixing, it is recommended to be stored away from light at 2℃ to 8℃ and used up within two weeks, and attention shall be paid to operate in sterile conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eResearch use only.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eThe culture example is only for reference, and the optimization of the culture procedure is \u003c\/strong\u003e\u003cstrong\u003edetermined by users based on experience.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6813531_6813532_6813533-Natural-Killer-Cells-Induction-Culture-Kit-2.0.pdf?v=1754032628\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e6813531、6813532、6813533-Natural-Killer-Cells-Induction-Culture-Kit-2.0.PDF\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003esuperculture\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"1L\/kit","offer_id":43398886424757,"sku":"6813533","price":498.0,"currency_code":"USD","in_stock":true},{"title":"2L\/kit","offer_id":43398886391989,"sku":"6813532","price":943.0,"currency_code":"USD","in_stock":true},{"title":"3L\/kit","offer_id":43398886359221,"sku":"6813531","price":1315.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6113031-SUPERCULTURE_NKKit2.0.jpg?v=1732877826"},{"product_id":"gf20-msc-xeno-free-sfm-superculture™","title":"Biofargo GF20 MSC Xeno-Free SFM","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCulture TM GF20 MSC Xeno-Free SFM is a medium for Human Mesenchymal Stem Cells (hMSCs), which contains no serum or animal origin compositions. It can be used for primary culture and subsequent amplification and passage culture of mesenchymal stem cells from the tissues such as human bone marrow, umbilical cord and fat, and maintain their trilineage differentiation potential into mesoderm. This product has superior proliferation promoting ability and short doubling time, which can save precious primary cells and experiment time for you.\u003c\/p\u003e\n\u003cp\u003eThis product was previously listed under catalog numbers 6914112-1 and 6914112-2, which have now been updated to 6914112 and 6914122, respectively, for improved product management.\u003c\/p\u003e\n\u003ch2\u003eProduct Information\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 15.7678%; height: 18px; text-align: center;\"\u003eType\u003c\/td\u003e\n\u003ctd style=\"width: 19.7582%; height: 18px; text-align: center;\"\u003eSpecification\u003c\/td\u003e\n\u003ctd style=\"width: 24.474%; height: 18px; text-align: center;\"\u003eCompositions\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eQuantity\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eVolume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 15.7678%; height: 18px; text-align: center;\"\u003eTypeA\u003c\/td\u003e\n\u003ctd style=\"width: 19.7582%; height: 18px; text-align: center;\"\u003e500 mL\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 24.474%; height: 18px; text-align: center;\"\u003eM200 Serum-Free\u003cbr\u003eMedium (TypeA)\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 bottle\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e490 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 15.7678%; height: 18px; text-align: center;\"\u003eTypeA\u003c\/td\u003e\n\u003ctd style=\"width: 19.7582%; height: 18px; text-align: center;\"\u003e500 mL\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 24.474%; height: 18px; text-align: center;\"\u003eF20 MSC Supplement\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 bottle\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 15.7678%; height: 18px; text-align: center;\"\u003eType B\u003c\/td\u003e\n\u003ctd style=\"width: 19.7582%; height: 18px; text-align: center;\"\u003e500 mL\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 24.474%; height: 18px; text-align: center;\"\u003eM200 Serum-Free\u003cbr\u003eMedium (Type B)\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 bottle\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e490 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 15.7678%; height: 18px; text-align: center;\"\u003eType B\u003c\/td\u003e\n\u003ctd style=\"width: 19.7582%; height: 18px; text-align: center;\"\u003e500 mL\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 24.474%; height: 18px; text-align: center;\"\u003eF20 MSC Supplement\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 bottle\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e* Type A represents \"with phenol red\", and Type B represents \"no phenol red\".\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eProduct Parameters\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90.6652%; height: 126px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eSuperCulture™ GF20 MSC Xeno-Free SFM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eClassification\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eSerum-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eSodium Glutamate\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eContaining glutamine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eAntibiotics\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eAntibiotics-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eHEPES Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eHEPES-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eSodium Bicarbonate Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003eContaining sodium bicarbonate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.5572%; height: 18px; text-align: center;\"\u003eEndotoxin Level\u003c\/td\u003e\n\u003ctd style=\"width: 53.1095%; height: 18px; text-align: center;\"\u003e\u0026lt; 0.24 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Conditions and Period of Validity\u003c\/h2\u003e\n\u003cp\u003eThis kit is composed of two compositions, each with different storage requirements.\u003cbr\u003eThese compositions are not sold separately:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe M200 Serum-Free Medium will keep for 1 year if stored away from light at 2℃ to 8℃;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe F20 MSC Supplement will keep for 1 year if stored away from light at -80℃ to -20℃;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe mixture of the two will keep for 2 weeks if stored at 2℃ to 8℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe product adopts the serum-free medium, which greatly reduces the variation between batches;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product contains no exogenous animal protein composition, which greatly reduces the risk of contamination by mycoplasma and the like;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product is suitable for hMSCs primary separation and subsequent amplification and passage culture of human mesenchymal stem cells from a variety of tissues such as umbilical cord, bone marrow and fat, and the cultured cells can be used for production or scientific research;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product has high amplification efficiency, which saves the culture time;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product can maintain the trilineage differentiation capacity of hMSCs;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAculture plate does not need to be wrapped in the culture process.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e Medium Preparation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. It is recommended to thaw the F20 MSC Supplement at room temperature (15℃ to 25℃) or thaw it overnight in a refrigerator (2℃ to 8℃). If necessary, the Supplement can be subpackaged in sterile conditions and stored at -80℃ to -20℃.\u003c\/p\u003e\n\u003cp\u003e2. Thaw 10 mL of F20 MSC Supplement completely in sterile conditions, and add it into 490 mL of M200 Serum-Free Medium to prepare a complete medium with a total volume of 500 mL.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eTips: If necessary, the user can prepare a required dosage in proportion, or add antibiotics by oneself, for example, add penicillin\/streptomycin into the complete medium at a dilution ratio of 1:100.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell Culture:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Count the Human Mesenchymal Stem Cells (hMSCs) harvested from resuscitation or passage;\u003c\/p\u003e\n\u003cp\u003e2. Add the medium to a desired cell concentration and inoculate the cells into a culture bottle (the generally recommended cell inoculation density is 0.4-0.8×10 4 cells\/cm 2 ) for culture.\u003c\/p\u003e\n\u003cp\u003e3. Culture Conditions: 5%CO2, and 37℃;\u003c\/p\u003e\n\u003cp\u003e4. Change the medium every 2-3 days according to the cell growth, and the cells can be digested when they are 80%-90% confluent;\u003c\/p\u003e\n\u003cp\u003e5. Cell Dissociation Method: Pour out the medium in the culture bottle, and clean the cells stuck to the wall twice with PBS or normal saline; it is recommended to spread 0.05% trypsin or a trypsin substitute (e.g., Biosci TM Trypsin Solution) over the bottom of the culture bottle; incubate the cells in an incubator at 37℃ for 1-3 min, add the complete medium with a dosage of more than 5 times of that of the trypsin to terminate digestion, transfer the cell suspension into a centrifugal tube, and conduct centrifugation at 300×g for 5 min. The precipitate is the required cells.\u003c\/p\u003e\n\u003cp\u003e6. Count the cells, and harvest them directly, or further inoculate and culture them as required.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eIf the Supplement in this product cannot be used up at one time, it can be subpackaged and stored in a freezing mode, but it shall not be frozen and thawed repeatedly, and shall be used within the validity of the product. After the complete medium is prepared by mixing, it is recommended to be stored away from light at 2℃ to 8℃ and used up within two weeks, and attention shall be paid to operate in sterile conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAs the product has high amplification efficiency, the recommended cell inoculation density is 0.4-0.8×10 4 cells\/cm 2 , and the user may determine the cell inoculation density as required and according to the actual situation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCompared with a medium containing serum, this product contains no serum, so the trypsin digestion terminating effect is poor, and excessive trypsin residue will cause damage to cells. Therefore, it is recommended to use 0.05% trypsin or a trypsin substitute for digestion when this product is used for cell culture and passage, thus to reduce the damage to cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe cell culture effect of this product may vary depending on cell sources, storage conditions, sample quality and operator experience.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eResearch use only.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6914112_6914122_SUPERCULTURE_GF20_MSC_Xeno-Free_SFM.pdf?v=1706491110\" target=\"_blank\"\u003eGF20 MSC Xeno-Free SFM-SuperCulture™\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003exeno,superculture\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"with phenol red","offer_id":43399005601973,"sku":"6914112","price":356.0,"currency_code":"USD","in_stock":true},{"title":"no phenol red","offer_id":43399005634741,"sku":"6914122","price":356.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6914112-GF20_TypeA_withphenolredSUPERCULTURE_GF20MSCXeno-FreeSFM_1.png?v=1732873014"},{"product_id":"newflash-protein-anykd-color-page-kit-biosci™","title":"NewFlash Protein AnyKD Color PAGE Kit-Biosci™","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eProduct Description\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eBiosci™ NewFlash Protein AnyKD Color PAGE kit is a product with a low acrylamide concentration, and adopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation rate. The kit is easy to use, prepares gel quickly and safely, no TEMED reagent, no special smell, and provides color stacking gel (color top gel), which is easy to sample.\u003c\/p\u003e\n\u003cp\u003eProtein gel coagulates quickly, and the entire gel making process can be completed in 25 min.\u003c\/p\u003e\n\u003cp\u003eProtein gel electrophoresis can be completed either at 300 V constant high voltage in about 25 min or at normal voltage.\u003c\/p\u003e\n\u003cp\u003eThe kit is suitable for separation and identification of 10-250 kDa protein, and there is no need to adjust the concentration of resolving gel according to protein molecular weight.\u003c\/p\u003e\n\u003cp\u003eColor stacking gel is easy to sample and remove after electrophoresis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eStorage and Transportation\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eStored at 2~8℃ protected from light, with a shelf life of 24 months.\u003c\/p\u003e\n\u003cp\u003eTransported on blue ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eApplicability\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eFor Research Use Only.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eProduct Composition\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.9686%;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 81.9686%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003e8012021\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e\n\u003cp\u003e8012031\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e\n\u003cp\u003e8012041\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e\n\u003cp\u003e8012051\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eComponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003ePGR\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e\n\u003cp\u003ePGY\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e\n\u003cp\u003ePGG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e\n\u003cp\u003ePGP\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eStacking\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×50\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003e√\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eStacking\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×50\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\n\u003cp\u003eRed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\n\u003cp\u003eYellow\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\n\u003cp\u003eGreen\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\n\u003cp\u003ePurple\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eResolving\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×125\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eResolving\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×125\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eAmmoniu\u003c\/p\u003e\n\u003cp\u003em\u003c\/p\u003e\n\u003cp\u003ePersulfate\u003c\/p\u003e\n\u003cp\u003e(APS)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e0.5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eThe number of gels prepared per kit\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90.2273%; height: 124px;\" data-mce-style=\"border-collapse: collapse; width: 90.2273%; height: 124px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 78px;\" data-mce-style=\"height: 78px;\"\u003e\n\u003ctd style=\"width: 18.1034%; height: 78px; text-align: center;\" data-mce-style=\"width: 18.1034%; height: 78px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.9656%; height: 78px; text-align: center;\" data-mce-style=\"width: 23.9656%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e0.75 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9655%; height: 78px; text-align: center;\" data-mce-style=\"width: 23.9655%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e1.0 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.2759%; height: 78px; text-align: center;\" data-mce-style=\"width: 24.2759%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e1.5 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 46px;\" data-mce-style=\"height: 46px;\"\u003e\n\u003ctd style=\"width: 18.1034%; height: 46px; text-align: center;\" data-mce-style=\"width: 18.1034%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003eNumber\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9656%; height: 46px; text-align: center;\" data-mce-style=\"width: 23.9656%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e62 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9655%; height: 46px; text-align: center;\" data-mce-style=\"width: 23.9655%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e50 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.2759%; height: 46px; text-align: center;\" data-mce-style=\"width: 24.2759%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e33 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003ePrecautions\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003e1.This product contains a small amount of acrylamide which is corrosive. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/p\u003e\n\u003cp\u003e2.This product is only used for scientific research by professionals and shall neither be used for clinical diagnosis, treatment, food or drugs nor stored in ordinary housing.\u003c\/p\u003e\n\u003cp\u003e3.\u003cstrong\u003eAdd 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS solution, and repack the solution into small portions for storage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eOperating Instructions\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003e1. With Biosci™ NewFlash Protein AnyKD Color PAGE kit, uniformly mix resolving gel\u003cbr\u003esolutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 2.0\/2.5\/3.8 mL of resolving gel solutions A and B). Gel preparation can be completed in a 15 mL or 50 mL centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e2. With Biosci™ NewFlash Protein AnyKD Color PAGE kit, uniformly mix stacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 0.8\/1.0\/1.5 mL of stacking gel solutions A and B).\u003c\/p\u003e\n\u003cp\u003e3. Add 10% APS solution to the resolving gel solution in step 1 (for 5 mL of resolving gel solution, add 50 µL of 10% APS solution), and pour the uniformly mixed resolving gel solution into the glass plate to a position 1.5 cm from the top of the front glass plate or 0.5 cm from teeth of a comb. Quickly and carefully add water, anhydrous ethanol, or isopropyl alcohol to cover the resolving gel solution. Alternatively, you can proceed directly to Step 5 without sealing the resolving gel solution, so that the liquid level of the resolving gel solution and the stacking gel solution is not very smooth, which is a normal phenomenon, does not affect the electrophoretic results. \u003cbr\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. After the resolving gel solution has solidified (3~10 min), pour out the covering liquid, wash the top of the resolving gel solution with deionized water for several times to remove the unpolymerized gel, remove the top liquid, and absorb the residual deionized water with filter paper.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e5. Add 10% APS solution to the stacking gel solution in step 2 (for 2 mL of stacking gel solution, add 20 µL of 10% APS solution), and directly pour the uniformly mixed stacking gel solution on the resolving gel solution. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization. Note: Gels can be stored for several weeks at 2~8℃ in sealing bags with a small amount of electrophoresis buffer.\u003c\/p\u003e\n\u003cp\u003e6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette for sample loading.\u003c\/p\u003e\n\u003cp\u003e7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis buffer. The voltage can be set to a maximum of 300 V, at which electrophoresis can be completed in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if the current is too high (to avoid much heat). Note: The electrophoresis of this product can be carried out at normal voltage.\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"Default Title","offer_id":43399030374581,"sku":"8012031","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012031BioSci_NewFlashProteinAnyKDColorPAGEKit_1.png?v=1732878264"},{"product_id":"serum-free-cell-freezing-medium-2-instructions-biosci™","title":"Biofargo Serum-Free Cell Freezing Medium (2x) Instructions","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eSerum-Free Cell Freezing Medium (2×) is a chemically defined freezing medium developed by Dakewe on the basis of lymphocyte freezing kit, which has clear components and contains neither xenogeneic animal-derived components nor serum. On the basis of obtaining high cell viability and cell yield, this product can effectively avoid the change of serum quality and the impact of serum components and exogenous components on experimental research, and achieve excellent cell freezing and thawing effects.\u003c\/p\u003e\n\u003ch2\u003eProduct Information\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.9699%; text-align: center;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 38.6509%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 30.3791%; text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.9699%; text-align: center;\"\u003e6032011\u003c\/td\u003e\n\u003ctd style=\"width: 38.6509%; text-align: center;\"\u003eSerum-Free Cell Freezing Medium (2×)\u003c\/td\u003e\n\u003ctd style=\"width: 30.3791%; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eDirection for Use\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eReagents and instruments requiring self-preparation by the experimenter:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eCell medium (commonly used for culturing cells requiring freezing);\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eClean bench;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTemperature-controlled centrifuge, equipped with horizontal rotor with 15 mL and 50 mL centrifuge tubes, and recommended use of professional cell centrifuge; the sterile conical tube should be used as the centrifuge tube;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eP20, P200 and P1000 micropipettes and pipette tips, pipette aids and sterile serological pipettes;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSterile 0.5 mL and 1.5 mL EP tubes;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell counter;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFreezing container and sterile cryotube;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e-80℃ freezer;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLiquid nitrogen tank;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWater bath at 37°C.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eCell Freezing\u003c\/h3\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Mark the cryotubes to be used in detail. Place the cryotubes at -20℃ for thermal equilibration, and place the freezing container, cell medium and serum-free cell freezing medium at 4℃ for thermal equilibration.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Count the cells to be frozen, and centrifuge 250 g at 4°C for 10 min.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. (Operation on ice) Discard the supernatant and resuspend with the cell medium equilibrated at 4℃ according to the results of cell count, so that the cell concentration is adjusted to twice the freezing concentration (e.g., if the cell concentration to be frozen is 1 × 10 7 cells\/mL, adjust the cell concentration to 2 × 10 7 cells\/mL with the medium), and then blow the cells off with the pipette evenly.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: The density of freezing cells recommended for this product is 5 × 10 5 - 2× 10 7 cells\/mL, and the appropriate density of freezing cells should be selected according to the nature of freezing cells and subsequent experimental arrangements during freezing.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. (Operation on ice) Slowly add an equal volume of serum-free cell freezing medium after placement in an ice bath dropwise, and mix gently.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: Be sure to add dropwise to avoid rapid changes in DMSO concentration in the medium.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. (Operation on ice) Dispense 1 mL of cell suspension per tube into freezing tubes equilibrated at -20°C, and then screw the cap tightly on the freezing tubes.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e6. Immediately place the freezing tube in the freezing container that has been equilibrated at 4℃, and then place the freezing container in a -80℃ freezer. Freezing tubes can also be placed in a programmable cooler to cool to -80℃ at a rate of 1℃\/min.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e7. Transfer freezing tubes stored at -80°C to liquid nitrogen 24 h later.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e8. Record the cell freezing location in the liquid nitrogen tank on the liquid nitrogen equipment management sheet in the laboratory.\u003c\/p\u003e\n\u003ch3\u003eCell Thawing\u003c\/h3\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Perform thermal equilibration on cell medium at 4°C. Perform thermal equilibration on 50 mL and 15 mL centrifuge tubes at 4℃. Preadjust the water bath to 37℃.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Remove the cryotubes from liquid nitrogen with forceps. If liquid nitrogen leaks into the freezing tube, unscrew the cap slightly to allow the vaporized liquid nitrogen to escape.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eWarning\u003c\/strong\u003e: Some freezing tubes have been reported to explode upon thawing, and to eliminate this hazard, it is recommended that only firm polyethylene freezing tubes be used for liquid nitrogen preservation in experiments.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. Clamp the freezing tube with forceps and immerse it in the water bath at 37℃ (note that the pipette tip should not be immersed below the liquid level). Gently shake the freezing tube and pay attention to the thawing of freezing cells in the tube. When the ice nucleus becomes soybean-sized in the tube, take it out, wipe the tube body with alcohol cotton, and transfer it to a clean bench.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: This step is critical for cell viability. It is necessary to thaw as soon as possible to reduce the damage to the cells during thawing; in addition, the time of cells staying at high temperature should be reduced as much as possible to reduce the toxicity of DMSO to cells. Do not leave the freezing tube in the water bath.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. (Operation on ice) Open the cap of the freezing tube. Gently pipette the cell suspension (approximately 1 mL) from the freezing tube into a precooled 15 mL centrifuge tube. Slowly add 5 mL of cell medium (4℃), gently shake while adding, cover the tube cap upon completion of dropping, and mix well by gentle inversion.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. Continue to add fill up with the medium slowly (4℃) to 14 mL, screw the cap, and mix by gentle inversion.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e6. Centrifuge 250 g for 10 min at 4°C. Carefully aspirate the supernatant, and the supernatant is required to be aspirated as far as possible without touching the cells pelleted in the tube bottom. Disperse the cell mass by flicking the finger in the bottom of the centrifuge tube. Add 1 mL of medium and resuspend cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: Aspirating the supernatant is more effective than decanting the supernatant, contributing to reduce the residual DMSO; finger flicking of the centrifuge tube can gently disperse the cell mass. Do not blow and aspire with a gun, otherwise it is harmful to the cells.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e7. (Optimization step, can be omitted) Cell clumping is caused by entanglement of DNA released from dead cells. 0.1 mL of DNase (1 mg\/mL) can be added and incubated at 37°C for 10 min.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e8. Take 50 μL of cell suspension and count trypan blue viable cells to calculate viability. Description: Viability = number of viable cells\/total number of cells × 100%. In the presence of trypan blue, viable cells are colorless and translucent, while dead cells are blue and dull.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e9. According to the counting results, fill up with cell medium to the desired cell concentration for subsequent assay.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eKeep away from light at 2~8℃, valid for 12 months.\u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freezing and thawing of this product, and pay attention to operate aseptically during use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAssociated Product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 234px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6911011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L100 Lymphocyte Serum-Free Medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6111021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L500 Lymphocyte Serum-Free Medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6811021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L500 Lymphocyte Serum-Free Medium\u003cbr\u003e(GMP)\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7922112\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman peripheral blood lymphocyte separation tube\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e15 mL × 20 vials, sieve\u003cbr\u003eplate tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7922021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman peripheral blood lymphocyte separation tube\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e50 mL × 25 vials, sieve\u003cbr\u003eplate tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7912011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eDensity reagent\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e250 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7111011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman lymphocyte separation medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e100 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6122012\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERGROW™ Cell Culture Supplemental Mix\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e25 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6122011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERGROW™ Cell Culture Supplemental Mix\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e250 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813521\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e3 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813522\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e2 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813523\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6032011_BioSci_Serum-Free_Cell_Freezing_Medium_2x.pdf?v=1706174101\" type=\"application\/pdf\" target=\"_blank\"\u003eSerum-Free Cell Freezing Medium (2×) Instructions-BioSci™\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003esuperculture\u003c\/div\u003e","brand":"Biofargo","offers":[{"title":"100mL\/Bottle","offer_id":43547255013557,"sku":"6032011","price":232.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_499aa19b-0292-4cde-852d-f2195dcd3d11.jpg?v=1753176103"},{"product_id":"dpbs-biosci™","title":"DPBS-BioSci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eDPBS, called “Dulbecco's Phosphate Buffered Saline”, is a balanced salt solution widely used in biology.\u003cbr\u003eThis DPBS does not contain calcium and magnesium, and can be used for washing cells\u003cbr\u003ebefore dissociation, diluting cells for counting, preparing reagents, etc.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003e1×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003ePhenol Red\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003eNo Phenol Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003eCalcium\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003eNo Calcium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003eMagnesium\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003eNo Magnesium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003epH\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003e7.0-7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 44.6824%; text-align: center;\"\u003eOsmolality\u003c\/td\u003e\n\u003ctd style=\"width: 55.3176%; text-align: center;\"\u003e270~300 mOsmol\/kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e  \u003cbr\u003e \u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 126px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 51.0341%; height: 36px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 48.9659%; height: 36px; text-align: center;\"\u003eConcentration\u003cbr\u003e(g\/L)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 51.0341%; height: 18px; text-align: center;\"\u003ePotassium Chloride (KCl)\u003c\/td\u003e\n\u003ctd style=\"width: 48.9659%; height: 18px; text-align: center;\"\u003e0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 51.0341%; height: 36px; text-align: center;\"\u003ePotassium Phosphate\u003cbr\u003emonobasic (KH 2 PO 4 )\u003c\/td\u003e\n\u003ctd style=\"width: 48.9659%; height: 36px; text-align: center;\"\u003e0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 51.0341%; height: 18px; text-align: center;\"\u003eSodium Chloride (NaCl)\u003c\/td\u003e\n\u003ctd style=\"width: 48.9659%; height: 18px; text-align: center;\"\u003e8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 51.0341%; height: 18px; text-align: center;\"\u003eSodium Phosphate dibasic\u003cbr\u003e(Na 2 HPO 4 )\u003c\/td\u003e\n\u003ctd style=\"width: 48.9659%; height: 18px; text-align: center;\"\u003e1.15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStored at room temperature, the shelf life is 24 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cp\u003ePay attention to operate in an aseptic condition.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6062011_BioSci_DPBS.pdf?v=1706174924\" type=\"application\/pdf\" target=\"_blank\"\u003eDPBS-BioSci™\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500mL\/Bottle","offer_id":43547312324789,"sku":"6062011","price":21.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6062011-DPBS.png?v=1732870765"},{"product_id":"dulbecco-s-modified-eagle-s-medium-high-glucose-no-hepes","title":"Biofargo Dulbecco's Modified Eagle's Medium-High Glucose-No HEPES - USDA","description":"\u003ch3\u003e\u003cstrong\u003eNote：Four times the concentration of aminoacids and vitamins,high-glucose type (4500 mg\/L),without animal-derived components.\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eDulbecco's Modified Eagle's Medium (DMEM) is a modified Eagle Basal cell medium (BME) containing four times the concentration of amino acids and vitamins.This product is a high-glucose type (4500 mg\/L) and supports the cultivation of various mammalian cells, including primary fibroblasts, neurons, gliocytes, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7 and PC-12. This product is a GMP grade medium without animal-derived components. Therefore, additional culture additives such as fetal bovine serum (FBS) need to be supplemented when culturing cells.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 88.8709%;\" data-mce-style=\"border-collapse: collapse; width: 88.8709%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003e500mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eSupplementation\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eNeed additional\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eGlutamine\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eL-Glutamine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003ePhenol Red\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003ePhenol Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eAntibiotics\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eNo Antibiotics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eSodium Pyruvate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eSodium Bicarbonate\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eSodium Bicarbonate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eHEPES Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003eNo HEPES\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003epH\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003e7.0-7.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eOsmolality\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003e295~335 mOsmol\/kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 43.5526%; text-align: center;\" data-mce-style=\"width: 43.5526%; text-align: center;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd style=\"width: 45.0621%; text-align: center;\" data-mce-style=\"width: 45.0621%; text-align: center;\"\u003e\u0026lt;0.3 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eIntended Use\u003c\/h2\u003e\n\u003cp\u003eThe medium is produced by aseptic processes and can be used for further manufacturing purposes. This product is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFormulation\u003c\/h2\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents (mg\/L)\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003cstrong\u003eAmino Acids\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 70.7451%; height: 270px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 70.7451%; height: 270px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eGlycine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Arginine•HCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e84\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Cystine•2HCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e63\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Glutamine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e584\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Histidine•HCl•H 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e42\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Isoleucine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Leucine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e105\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Lysine•HCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e146\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Methionine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Phenylalanine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e66\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Serine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e42\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Threonine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e95\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Tryptophan\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e16\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Tyrosine•2Na•2H 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e104\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39%; height: 18px; text-align: center;\" data-mce-style=\"width: 39%; height: 18px; text-align: center;\"\u003eL-Valine\u003c\/td\u003e\n\u003ctd style=\"width: 26.8439%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.8439%; height: 18px; text-align: center;\"\u003e94\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cstrong\u003eVitamins\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 71.2196%;\" data-mce-style=\"border-collapse: collapse; width: 71.2196%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eCholine chloride\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eFolic Acid\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003emyo-Inositol\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e7.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eNiacinamide\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eD-Pantothenic Acid•1\/2 Ca\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003ePyridoxal•HCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eRiboflavin\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e0.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.5126%; text-align: center;\" data-mce-style=\"width: 37.5126%; text-align: center;\"\u003eThiamine•HCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.4874%; text-align: center;\" data-mce-style=\"width: 26.4874%; text-align: center;\"\u003e4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eInorganic Salts\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 71.4442%; height: 125px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 71.4442%; height: 125px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eCaCl₂\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eFe(NO₃)₃•9H₂O\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e0.1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eMgSO₄\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e97.67\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eKCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eNaHCO₃\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e3700\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\" data-mce-style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 17px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 17px; text-align: center;\"\u003eNaCl\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 17px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 17px; text-align: center;\"\u003e6400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 36.523%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.523%; height: 18px; text-align: center;\"\u003eNaH₂PO₄\u003c\/td\u003e\n\u003ctd style=\"width: 26.477%; height: 18px; text-align: center;\" data-mce-style=\"width: 26.477%; height: 18px; text-align: center;\"\u003e109\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cstrong\u003eOther\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 71.4538%; height: 54px;\" data-mce-style=\"border-collapse: collapse; width: 71.4538%; height: 54px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39.001%; height: 18px; text-align: center;\" data-mce-style=\"width: 39.001%; height: 18px; text-align: center;\"\u003eD-Glucose\u003c\/td\u003e\n\u003ctd style=\"width: 27.999%; height: 18px; text-align: center;\" data-mce-style=\"width: 27.999%; height: 18px; text-align: center;\"\u003e4500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39.001%; height: 18px; text-align: center;\" data-mce-style=\"width: 39.001%; height: 18px; text-align: center;\"\u003ePhenol Red•Na\u003c\/td\u003e\n\u003ctd style=\"width: 27.999%; height: 18px; text-align: center;\" data-mce-style=\"width: 27.999%; height: 18px; text-align: center;\"\u003e15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 39.001%; height: 18px; text-align: center;\" data-mce-style=\"width: 39.001%; height: 18px; text-align: center;\"\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd style=\"width: 27.999%; height: 18px; text-align: center;\" data-mce-style=\"width: 27.999%; height: 18px; text-align: center;\"\u003e110\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStored at 2℃~8℃, away from light, the shelf life is 12 months.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cp\u003eAvoid repeated freezing and thawing.\u003cbr\u003ePay attention to operate in an aseptic condition.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n","brand":"Biofargo","offers":[{"title":"500mL\/Bottle","offer_id":43725097074869,"sku":"6016111","price":30.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_5f972b74-0ec0-493d-b243-a94caa24f319.jpg?v=1753174860"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/Sale.png?v=1741877815","url":"https:\/\/biofargo.com\/collections\/on-sales.oembed?page=4","provider":"Biofargo","version":"1.0","type":"link"}