{"title":"LAMP Kits","description":"\u003cp\u003eLoop-mediated isothermal amplification (LAMP) is a cost-effective nucleic acid amplification method that delivers rapid results without the need for expensive equipment. It is widely used for detecting pathogens like bacteria, viruses, and protozoans in food and plant samples.\u003c\/p\u003e","products":[{"product_id":"4xlamp-mastermix-electrophoresis-50-kit","title":"4x LAMP MasterMix (Electrophoresis, 50\/kit)","description":"\u003ch2\u003eProduct Introduction:\u003c\/h2\u003e\n\u003cp\u003eThe 4×LAMP MasterMix contains all the components required for LAMP amplification, and can be used for LAMP isothermal amplification, which is Reverse Transcription Loop-Mediated Isothermal Amplification and utilizes 4 template-specific. It uses 4 template-specific primers, reverse transcriptase and Bst DNA polymerase 2.0 (8U\/uL) with strand-swapping ability to complete reverse transcription and LAMP amplification in 30-60 minutes at isothermal conditions (around 65°C). This technique is comparable to or better than qualitative PCR in terms of sensitivity, specificity and detection range, without the need for expensive instrumentation. It has been successfully applied to rapid biological assays and is widely used in various fields.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eProduct Features:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReady-to-use, contains red electrophoresis tracer, can be directly sampled after amplification, no need for sample loading solution.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003e4× concentration, providing more space for adding samples, which can increase the chance of detection.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eHigh specificity, carefully optimized formulation, non-specific amplification is lower than self-assembled reagents.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ehigh amplification efficiency, generally an order of magnitude more sensitive than PCR.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eIsothermal amplification around 65°C, no need for expensive instruments.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe kit is sufficient for 50 times of LAMP amplification of 20uL system.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe amplification control is provided to facilitate the analysis of the cause when difficulties are encountered.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThis product can only be used for research, not for clinical use.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents: \u003c\/h2\u003e\n\u003cstyle type=\"text\/css\"\u003e\u003c!--\n\ttable.tableizer-table {\n\t\tfont-size: 12px;\n\t\tborder: 1px solid #CCC; \n\t\tfont-family: Arial, Helvetica, sans-serif;\n\t} \n\t.tableizer-table td {\n\t\tpadding: 4px;\n\t\tmargin: 3px;\n\t\tborder: 1px solid #CCC;\n\t}\n\t.tableizer-table th {\n\t\tbackground-color: #104E8B; \n\t\tcolor: #FFF;\n\t\tfont-weight: bold;\n\t}\n--\u003e\u003c\/style\u003e\n\u003ctable class=\"tableizer-table\" style=\"width: 549px;\"\u003e\n\u003cthead\u003e\n\u003ctr class=\"tableizer-firstrow\"\u003e\n\u003cth style=\"width: 233.78125px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 89.21875px;\"\u003eCategory No.\u003c\/th\u003e\n\u003cth style=\"width: 44px;\"\u003eUnit Size\u003c\/th\u003e\n\u003cth style=\"width: 130px;\"\u003ePackaging Material\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 233.78125px;\"\u003e4×LAMP MasterMix \u003cbr\u003e(with electrophoretic tracer, enzyme to be added)\u003c\/td\u003e\n\u003ctd style=\"width: 89.21875px;\"\u003e211220a\u003c\/td\u003e\n\u003ctd style=\"width: 44px;\"\u003e200uL\u003c\/td\u003e\n\u003ctd style=\"width: 130px;\"\u003e0.5 mL tube w\/ white cap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 233.78125px;\"\u003eBst DNA polymerase 2.0(8U\/uL)\u003c\/td\u003e\n\u003ctd style=\"width: 89.21875px;\"\u003e11-220319\u003c\/td\u003e\n\u003ctd style=\"width: 44px;\"\u003e50uL\u003c\/td\u003e\n\u003ctd style=\"width: 130px;\"\u003e0.5 mL tube w\/ red cap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 233.78125px;\"\u003ePositive control template - primer mixture\u003c\/td\u003e\n\u003ctd style=\"width: 89.21875px;\"\u003e210801\u003c\/td\u003e\n\u003ctd style=\"width: 44px;\"\u003e50uL\u003c\/td\u003e\n\u003ctd style=\"width: 130px;\"\u003e0.5 mL tube w\/ blue cap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 233.78125px;\"\u003eUser Manual\u003c\/td\u003e\n\u003ctd style=\"width: 89.21875px;\"\u003e211220sc\u003c\/td\u003e\n\u003ctd style=\"width: 44px;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 130px;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Bingene","offers":[{"title":"Default Title","offer_id":42043433779381,"sku":"211220","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/IMG_9152.png?v=1732864945"},{"product_id":"fluorescent-and-colorimetric-rt-lamp-kit","title":"Fluorescent and Colorimetric RT-LAMP Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv data-mce-fragment=\"1\" data-docx-has-block-data=\"true\" data-page-id=\"doxuswEYm94OYhhSTaHEo4V2Btd\"\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusmGCRuGmqCSaKEvtPJKchZb\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusTufKwQtG7rNgSrBxz9Oqxb\"\u003eBiofargo Fluorescent and Colorimetric RT-LAMP Kit is designed to provide a one-step solution for Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) of RNA and an examination of the amplification results with naked eyes and with real-time fluorescent detector like real-time PCR machines. It has the following features：\u003c\/div\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusAFeqTvAyL7LeANkpOu2IJf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eOptimized RT-LAMP mix, contains both fluorescent and colorimetric dyes that enables the end-point examination of RT-LAMP results with naked eyes and the real-time detection of the amplification with a real-time PCR machine.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusqbaIIPyGO3u5vSicZaAKxc\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eReal-time monitoring of the RT-LAMP reactions.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxushBbgJ7hkhaRosSys2fn5Yg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eUseful in optimizing RT-LAMP primers or reaction parameters.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus5ojvfKfKNCyeUHxyye5ENg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e4× concentration RT-LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusIphEuQZ2GkEdkuA8G22Bfl\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than RT-PCR.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusOTmknhyH0qUmMW777dhvAC\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusVxVbSxUsmLmMKYUOMw933d\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eTemplate-primer mix supplied as RT-LAMP positive control, eliminates false negative results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusYv6PUkP5EkXbfxSJTOg7FJ\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eCAT#211224-50 is sufficient for 50 reactions of 20uL volume, and CAT#211224-500 for 500 reactions of 20uL volume.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInput Sample Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusUZEAucSJn1KbtcXRhny2ec\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform RNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications，one is for positive control and another negative control.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuswg4k5pslocbNXNR78jQNVh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuswg4k5pslocbNXNR78jQNVh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus4OKzvRfKQr7hb6bM6wbHtf\"\u003eComponent\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus8DuSzGTliXjC0xAQaA9Ovh\"\u003eCAT#:211224-50\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusfeHK2LaayJQfdRp1rF89zh\"\u003eCAT#:211224-500\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusQpWGdwTuOvDAXGh2qbV1Xs\"\u003eFluorescent and Colorimetric RT-LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusVKfHNev4hCKOfyoLNQhTGg\"\u003e200μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusXL2FiwL3vna3xKTpjzU4R6\"\u003e1mL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusTVWJgujTxUz7wJ6hbxKLac\"\u003eRTase-Bst Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxushULuB2wfhCzy4FL5OIXuFc\"\u003e100μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusNIKlGG99AIEz9H78ETWxBf\"\u003e500μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2GNVuZznTcQbPRe4PyXkgb\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusJ0roRA7VcEHw33wYeWGnue\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusUxZrHasR0FMXhf9dLbYqle\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Handling\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuswg4k5pslocbNXNR78jQNVh\"\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusqtW2uTgtUWHiG2dtCSrWtd\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eRequired Materials not Supplied\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"365;365\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"365\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"365\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuskPyAN3HqDLjXBalOYD38ng\"\u003eNo\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusLAFUJjbERXAKHYsA7nbUuc\"\u003eItem\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusg7LCuob4BysiLDdtc520jh\"\u003e1\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusyvHXuh0svluHYsW93IiNkc\"\u003ePrepared RNA Samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusucTcaDWQEP8WkYKAUyvJDe\"\u003e2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusgBWal3SkSAq2cxgp8v84ag\"\u003eRT-LAMP primers targeting RNA samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusqDG6gdbZnI5jsBV60bVx0c\"\u003e3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMjpqPZhMPtHzKm1IXRrXxf\"\u003eReaction vessels compatible with instrument for RT-LAMP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusgSHIuwaTf8PKQKmbWFEYPe\"\u003e4\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusncjgnrpyuwusJimX7Ldzxh\"\u003eMolecular biology grade ddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuspQZzUiEbnrvHEX9eHsqKGb\"\u003e5\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus9QfpfgyekLwn4y2wPK9h2f\"\u003eReal-time PCR machine\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2LfiwPcJD3xamtfDXtAWmd\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2LfiwPcJD3xamtfDXtAWmd\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003eProcedural Guidelines\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusVYhpLW7nyqyJwlUiAoEzUf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusctn2cnXWK6lWeVqPgzP0Bd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusnOsvlA1r6XzFBS3B3eqguf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eAvoid opening reaction vessels after a RT-LAMP reaction is completed.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusKJfO7mIN8LB3dd9akcQ6yz\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eScreening at least 10 sets of RT-LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusDu0ecSm0iKm0Qyhy4l5OEh\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eUse Loop primers if possible.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusFIHqZXojdEmllrrf0nc8Ie\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePrimers should be HPLC purified.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuszpgD8W2EqLgjJ7COYp5Vhc\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuszpgD8W2EqLgjJ7COYp5Vhc\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProcedures\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusS8Qmh0C1NkIsG8zSy76fgg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e Make 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified RT-LAMP primers at 100μM each. Use the 100μM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100μL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusG0wZ4hZonuYdGIML8YLNnh\"\u003eComponents\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusiAfQ4LpEsKyaRMtSYjCHbb\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusXuL7a631U3qoexrnoeE9ec\"\u003eVolume\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusxtzL8c8wEAWiyH1ZcFDyvk\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusUSLO4xEhjFjVeQ1W42w1wf\"\u003eFinal Concentration\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusXsYX4MBpE5p0AtRWVikcPf\"\u003ein 20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMCER9sM1WqHHoNMSSdM4Xb\"\u003e100μM FIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxussbBMP4BHAUsdr2Pep2fWjd\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYBFTIXFDRQDuEo1ChKNqkc\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus5Ari0PgStxn5rQTtrD2D3x\"\u003e100μM BIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusrOatFyExgNQmYJ1vJ1CGKd\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus9H7wI9DHSDsHFjp2fedQtk\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusD4BFeFgM2nM5x5du9Rlice\"\u003e100μM Loop F\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusPdjW8smtGnnskRseOg1sXe\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusOgi5p3AJEGNRizMtp3TLrd\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusi7LfhyhpDAovhyidN9rY5g\"\u003e100μM Loop B\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusRycrwTO4vvSbrF2BzEZAxb\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuse3a0SmVlxl73t9Kh7BszXc\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusfLf0QbCGVcKSxmmyE71Dm6\"\u003e100μM F3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2mVZ1NlDpG0E4l5qk76xJw\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusqcYcNXRRTnnfNHIuvonKld\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuspptvi6YTrXIbLptkgCSkpe\"\u003e100μM B3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusiIOHKEG4S0A6LJ6HeZsAQg\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusAHa00grJxk5RA8S2Af7rGd\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusfrfMrqnOtZnE4yNqOQUu2c\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus4HfGxHT3Y40rQaYKEfX5rh\"\u003e24μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusSs78NG07nBdW8YiPq714Wb\"\u003e-\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusNgC9Dx0iU1VRIylnl47MmX\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThaw all components to be used at room temperature. Vortex briefly Fluorescent and Colorimetric RT-LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex RTase-Bst Mix.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusmnzONlUo3x7moubQd09jFl\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eWhen using the kit for the first time，add all RTase-Bst Mix to Fluorescent and Colorimetric RT-LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusziaJQwjXJGsoY7Ie6QPDIX\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eFor a purification number of N+2, set up N+4 RT-LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for RT-LAMP Positive Control (PC) and another for RT-LAMP Negative Control (NC) .\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"182;182;182;182\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusQZakT1UXd29zZYJ4bwhHUh\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6xVhbH5ouEj31lABK2LAle\"\u003ePC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMkX17EoUu9cDKsOpJUDB7e\"\u003eNC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusdz4Avi6089O72oDY4dYtgg\"\u003eN+2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusdotAshskBiSvIYW40YQ6he\"\u003eFluorescent and Colorimetric RT-LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusvFdJ7Gd3wSko8DEfwmVSYf\"\u003e6μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusS8yjoVOO1OXGIVFwwJBUAf\"\u003e6μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6aDHcJs0JJhiihYcn2vGMb\"\u003e6μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusZkFsS4USlLXuNkogiWf9pg\"\u003e20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus4mWCZ7j6JHapIcwBmCh9oh\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusAHi8XwuvW5U9RfXYjuHfrb\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusxqKAAvf47WCGbd0E6B5rxg\"\u003e1μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusvANsk6j0P7BmWRdpmUmrbc\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusoOImGLgS3vEctZtPe3sxVh\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusUDJlA1OvWIKqZ2YFVmjCRh\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusTE3JwP8nB80Moe5ioUHaSh\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusKy4aoAwqKnaBJQtTWHjFQf\"\u003eN+2 samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusL0k2nh2mM9dfX2jkGbB9Pg\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6fFHbwzWtCNzmkSqvBmaqd\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxushRixbzL1KbPfdp1fdHEbVh\"\u003e1-13μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusKb4ahDKkqEs34567GGmQBh\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus0dWro8yQUDPIOsIKWTkQxf\"\u003e8μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuslrskAIpLJhBdYLtlN3zv5c\"\u003e13μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuslmmmwekxbRGxuOL4vXatlc\"\u003eAdd to 20μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusMQr8RwAcpQ1f1n5CrHvWwe\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eSeal reaction vessels.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus2WBgVes6rkTrXfyUWatrse\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIncubate at 65°C for 60 minutes in a real-time PCR machine. Collect signal in the SYBR channel at every 60 seconds.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus3vljq02oikELxg5MJaj6Sf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eAfter the reaction is finished, remove tubes or vessels from incubation and examine by eye. The two positive reactions should have S-shaped fluorescent signal, the reaction mixtures will turn green. The two negative controls should have no S-shaped fluorescent signal and the reaction mixtures should turn light green.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"4\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxuscjNAqnAqhmjlk35xkhksPb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is amplification in NC or there is no amplification in PC，the whole experiment is invalid. There is no need to analysis the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"5\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusB54CvoTObmcv7wqEwfWTZg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is no amplification in NC or there is amplification in PC，the whole experiment is valid. Analyze and record he results of the N+2 samples. The results from fluorescent detection can be downloaded from the real-time PCR machine, the colorimetric results can be photographed or scanned for later use.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDqd9p0ncSeMOfrsRo8qSdh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDqd9p0ncSeMOfrsRo8qSdh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eTroubleshooting\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxuszyTYl4z5N6Nn6HPuwUcOYb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is amplification in NC.\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus1Jl6uxzv8oT4RgvJz9U4tg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-RT-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusxrTYbUE5XzgBSP8Nd0kwNb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C -70°C，or put the reaction tubes to 65°C as soon as the reaction is set，or use a new set of primer for the targeted RNA region.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxuspCh9zvGFEsKKEab8YZjbCd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is no amplification in PC\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusvSRaPrR7B6JJht1kndYHBc\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusw94nNUureY9VOLxMvXW3qf\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusw94nNUureY9VOLxMvXW3qf\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLimited Product Warranty\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusahsJKECmk4wEwG2AuYipGf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eBiofargo，Inc. warrants its products as set forth in the Biofargo’s General Terms and Conditions of Sale at www.biofargousa.com. If you have any questions, please contact Biofargo@ www.biofargo.com.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusbw8GRXHWm6tVpPpcHKxTPe\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThe information in this guide is subject to change without notice.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusTELuHfrJGK1kbFLGkVFuPc\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, Biofargo INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"lark-record-clipboard\" data-lark-record-format=\"docx\/record\" data-lark-record-data='{\"isCut\":false,\"rootId\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"parentId\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"blockIds\":[7,8,9,10,11,12,13,14,15,16,17,18,19,20,45,46,47,48,73,74,75,76,77,78,79,80,81,82,107,108,109,110,135,136,137,138,139,140,141,144,146,147,148,149],\"recordIds\":[\"doxusmGCRuGmqCSaKEvtPJKchZb\",\"doxusTufKwQtG7rNgSrBxz9Oqxb\",\"doxusAFeqTvAyL7LeANkpOu2IJf\",\"doxusqbaIIPyGO3u5vSicZaAKxc\",\"doxushBbgJ7hkhaRosSys2fn5Yg\",\"doxus5ojvfKfKNCyeUHxyye5ENg\",\"doxusIphEuQZ2GkEdkuA8G22Bfl\",\"doxusOTmknhyH0qUmMW777dhvAC\",\"doxusVxVbSxUsmLmMKYUOMw933d\",\"doxusYv6PUkP5EkXbfxSJTOg7FJ\",\"doxusRhIlXyLuUXF7c7EgDBIm7e\",\"doxusUZEAucSJn1KbtcXRhny2ec\",\"doxuswg4k5pslocbNXNR78jQNVh\",\"doxus1ztbEdbthIq7NDs9xWY8GQ\",\"doxusaHuFSGjypkCPlC2phXNSSf\",\"doxusqtW2uTgtUWHiG2dtCSrWtd\",\"doxusRZ4fcz6mrdzZiLRZMM015b\",\"doxusa0wgSvZgrxjkh3npjYlmef\",\"doxus2LfiwPcJD3xamtfDXtAWmd\",\"doxusVYhpLW7nyqyJwlUiAoEzUf\",\"doxusctn2cnXWK6lWeVqPgzP0Bd\",\"doxusnOsvlA1r6XzFBS3B3eqguf\",\"doxusKJfO7mIN8LB3dd9akcQ6yz\",\"doxusDu0ecSm0iKm0Qyhy4l5OEh\",\"doxusFIHqZXojdEmllrrf0nc8Ie\",\"doxuszpgD8W2EqLgjJ7COYp5Vhc\",\"doxusS8Qmh0C1NkIsG8zSy76fgg\",\"doxusyUfL8xcD0cKKRmfL1J0fhc\",\"doxusNgC9Dx0iU1VRIylnl47MmX\",\"doxusmnzONlUo3x7moubQd09jFl\",\"doxusziaJQwjXJGsoY7Ie6QPDIX\",\"doxusGG8lc0lgtnBEwx7CEXGCag\",\"doxusMQr8RwAcpQ1f1n5CrHvWwe\",\"doxus2WBgVes6rkTrXfyUWatrse\",\"doxus3vljq02oikELxg5MJaj6Sf\",\"doxuscjNAqnAqhmjlk35xkhksPb\",\"doxusB54CvoTObmcv7wqEwfWTZg\",\"doxusDqd9p0ncSeMOfrsRo8qSdh\",\"doxuszyTYl4z5N6Nn6HPuwUcOYb\",\"doxuspCh9zvGFEsKKEab8YZjbCd\",\"doxusw94nNUureY9VOLxMvXW3qf\",\"doxusahsJKECmk4wEwG2AuYipGf\",\"doxusbw8GRXHWm6tVpPpcHKxTPe\",\"doxusTELuHfrJGK1kbFLGkVFuPc\"],\"recordMap\":{\"doxusmGCRuGmqCSaKEvtPJKchZb\":{\"id\":\"doxusmGCRuGmqCSaKEvtPJKchZb\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Product Description\"},\"attribs\":{\"0\":\"*1*0+j\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"6985633702449512454\"]},\"nextNum\":2}},\"type\":\"text\"}},\"doxusTufKwQtG7rNgSrBxz9Oqxb\":{\"id\":\"doxusTufKwQtG7rNgSrBxz9Oqxb\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Biofargo Fluorescent and Colorimetric RT-LAMP Kit is designed to provide a one-step solution for Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) of RNA and an examination of the amplification results with naked eyes and with real-time fluorescent detector like real-time PCR machines. It has the following features：\"},\"attribs\":{\"0\":\"*1*0+9*1+b*1*2+h*1+6h*1*3+8*1+1o\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"grammarly-data\",\"{\\\"id\\\":\\\"a334d9b4-dbcf-480b-acdb-32b980c43e62\\\",\\\"operation\\\":\\\"delete\\\",\\\"origin_text\\\":\\\"Biofargo \\\",\\\"replace_text\\\":\\\"\\\",\\\"rule_id\\\":\\\"1004\\\",\\\"rule_description\\\":\\\"Possible Grammar Issue@202206090605350101000081950472EF74@en@0@0\\\",\\\"is_ignore\\\":0}\"],\"1\":[\"author\",\"6985633702449512454\"],\"2\":[\"grammarly-data\",\"{\\\"id\\\":\\\"12284493-e3cc-4ec4-ae42-96df3d096a46\\\",\\\"operation\\\":\\\"delete\\\",\\\"origin_text\\\":\\\" and Colorimetric\\\",\\\"replace_text\\\":\\\"\\\",\\\"rule_id\\\":\\\"1003\\\",\\\"rule_description\\\":\\\"Conjunction Suggestion@202206090605350101000081950472EF74@en@20@0\\\",\\\"is_ignore\\\":0}\"],\"3\":[\"grammarly-data\",\"{\\\"id\\\":\\\"ca944932-7d5d-4bce-82dd-80ad0038c1f3\\\",\\\"operation\\\":\\\"replace\\\",\\\"origin_text\\\":\\\"detector\\\",\\\"replace_text\\\":\\\"detectors\\\",\\\"rule_id\\\":\\\"1005\\\",\\\"rule_description\\\":\\\"Noun Form Suggestion@202206090605350101000081950472EF74@en@270@0\\\",\\\"is_ignore\\\":0}\"]},\"nextNum\":4}},\"type\":\"text\"}},\"doxusAFeqTvAyL7LeANkpOu2IJf\":{\"id\":\"doxusAFeqTvAyL7LeANkpOu2IJf\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"6985633702449512454\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+67\"},\"text\":{\"0\":\"Optimized RT-LAMP mix, contains both fluorescent and colorimetric dyes that enables the end-point examination of RT-LAMP results with naked eyes and the real-time detection of the amplification with a real-time PCR machine.\"}}},\"type\":\"bullet\"}},\"doxusqbaIIPyGO3u5vSicZaAKxc\":{\"id\":\"doxusqbaIIPyGO3u5vSicZaAKxc\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"6985633702449512454\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+1a\"},\"text\":{\"0\":\"Real-time monitoring of the RT-LAMP 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If you have any questions, please contact Bingene @ www.bingene.com.\"}}},\"type\":\"bullet\"}},\"doxusbw8GRXHWm6tVpPpcHKxTPe\":{\"id\":\"doxusbw8GRXHWm6tVpPpcHKxTPe\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"6985633702449512454\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+1v\"},\"text\":{\"0\":\"The information in this guide is subject to change without notice. \"}}},\"type\":\"bullet\"}},\"doxusTELuHfrJGK1kbFLGkVFuPc\":{\"id\":\"doxusTELuHfrJGK1kbFLGkVFuPc\",\"snapshot\":{\"align\":\"left\",\"author\":\"6985633702449512454\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuswEYm94OYhhSTaHEo4V2Btd\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, BINGENE INC. 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Title","offer_id":42191448604853,"sku":"211224-50","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_pcrkit.webp?v=1751468806"},{"product_id":"fluorescent-and-colorimetric-lamp-kit","title":"Fluorescent and Colorimetric LAMP Kit","description":"\u003cdiv data-docx-has-block-data=\"true\" data-page-id=\"doxusN45EF1y4IwhGqeXhFWRxqg\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusYfj7njjKdjGvCb58s7DC7f\"\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusiYkA47n9pHDavDvC5BLhXc\"\u003eBiofargo Fluorescent and Colorimetric LAMP Kit is designed to provide a one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA and an examination of the amplification results with naked eyes and with real-time fluorescent detector like real-time PCR machines. It has the following features：\u003c\/div\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusInlOnVpprrb2sz5Et3GTkf\"\u003e\n\u003cdiv\u003eOptimized LAMP mix, contains both fluorescent and colorimetric dyes that enables the end-point examination of LAMP results with naked eyes and the real-time detection of the amplification with a real-time PCR machine.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusrdlvF6PjtLnK2ohLEZ7xvd\"\u003e\n\u003cdiv\u003eReal-time monitoring of the LAMP reactions.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusoepusHeobLuYds9nxrtUFf\"\u003e\n\u003cdiv\u003eUseful in optimizing LAMP primers or reaction parameters.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusUkwSSyiPfAFTlPms4jaUQg\"\u003e\n\u003cdiv\u003e4× concentration LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusrO0Cw31Eq0p6QnRGgrOlkd\"\u003e\n\u003cdiv\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than PCR.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusDrgUag6WLdzl3y2AZVfdfe\"\u003e\n\u003cdiv\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusLdydHvTp0Ii8mpN0KCYqxh\"\u003e\n\u003cdiv\u003eTemplate-primer mix supplied as LAMP positive control, eliminates false negative results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusJ0QDIadlGHeSokfxzm8KJf\"\u003e\n\u003cdiv\u003eCAT#211223-50 is sufficient for 50 reactions of 20μL volume, and CAT#211223-500 for 500 reactions of 20μL volume.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusamKBl5tcEVM30BbpvHnbfg\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusamKBl5tcEVM30BbpvHnbfg\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusamKBl5tcEVM30BbpvHnbfg\"\u003e\u003cstrong\u003eInput Sample Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxuswGB0Yvhhec6RRUjgF3TWVh\"\u003e\n\u003cdiv\u003ePerform DNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications，one is for positive control and another negative control.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusogzvOYS5weHO37ohm20jNe\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusogzvOYS5weHO37ohm20jNe\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusogzvOYS5weHO37ohm20jNe\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv\u003e\n\u003ctable data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusoNwbDEzH45c937J7330Svg\"\u003eComponent\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusZJLq03QM5DLGgCKjDQkeUf\"\u003eCAT#:211223-50\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszahlKAHMf2mRvZqTQKUe1f\"\u003eCAT#:211223-500\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuscG8iwoCqTSfXcKiyXRerEf\"\u003e4×Fluorescent and Colorimetric LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusH8dAFLzh1M21StPd5Beftb\"\u003e200μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusZzFANJ747khKz4FSgWn9ad\"\u003e1mL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus0cDS9npVfGDohEi0zC7SPf\"\u003eBst DNA Polymerase 2.0\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHs6j9D1DIzKQ1Q5ROjPQGh\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusndEAmaMBavPtgm7iTbIvQe\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusrcd4GktYlyWDLoAkfvMF1g\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusDnLiCFtIpzRjOCm5LiF8oc\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusr5OGOZYPBWaiL52tSKO4lb\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eStorage and Handling\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusEKyzHpyAULRuWqQDEH5U9b\"\u003e\u003cstrong\u003eRequired Materials not Supplied\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv\u003e\n\u003ctable data-ace-table-col-widths=\"365;365\" class=\"ace-table\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"365\"\u003e \u003ccol width=\"365\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusb0Yiwa4N7ecW4gCabMxbez\"\u003eNo\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusmP6qp1W2zZbXanCT82eYse\"\u003eItem\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuspuOdKMxkQFp71ISyxvyaxc\"\u003e1\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusQYo6FEjDNj4aJNDj1vOZmh\"\u003ePrepared DNA Samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusERqFpCXZmyuFOCZiwaqtrN\"\u003e2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusKsdJNERXPOj1aNEEWG9kWb\"\u003eLAMP primers targeting DNA samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszojIGBW0gcH7dEOgjpgoCf\"\u003e3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2L5fJ9YVmcLoBCwjSAvw5K\"\u003eReaction vessels compatible with instrument for LAMP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusvAbhF9ZXHI1WhAYi0ybwob\"\u003e4\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusevV5pd1nFfErTdWGdbW9sc\"\u003eMolecular biology grade ddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusWjmCtHGvTGznmeOnvygjZe\"\u003e5\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusbwWVnDmjBwxmZHD5vEHkPe\"\u003eReal-time PCR machine\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eProcedural Guidelines\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusEjenHxfejYa6XyCoNC10jc\"\u003e\n\u003cdiv\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusk53ckd2nRyw0wqyeNFcJdg\"\u003e\n\u003cdiv\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusBEi6hKcjjv7ZxPs0SEyS4c\"\u003e\n\u003cdiv\u003eAvoid opening reaction vessels after a LAMP reaction is completed.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusDlw56ZrLKy5jKHB1AIqCPd\"\u003e\n\u003cdiv\u003eScreening at least 10 sets of LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusc41op3FUKawYpxDuzBsDef\"\u003e\n\u003cdiv\u003eUse Loop primers if possible.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxustYoLvl3XMYNaNKWXuafIuh\"\u003e\n\u003cdiv\u003ePrimers should be HPLC purified.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxuswmBxMZaIMQFqJdySFKUnUy\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxuswmBxMZaIMQFqJdySFKUnUy\"\u003e\u003cstrong\u003eProcedures\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus6hoGFN2PqOGvXb1Q3tVoae\"\u003e\n\u003cdiv\u003e Make 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified LAMP primers at 100μM each. Use the 100μM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100μL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv\u003e\n\u003ctable data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusq3f0xQZD2wcZSPYRBfU82e\"\u003eComponents\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusaSC88NfHNYfSIAtE9obrob\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusOwVjkRs3E5Myi2ho6QTI4W\"\u003eVolume\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusLif1UoaRs1funzH6UemqBW\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusb1XH0ujttzIjVqfUAAmctf\"\u003eFinal Concentration\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuswIoNO1f1Qn2gcVf5dI8t1c\"\u003ein 20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus1KTVeIMDn9adLNru6KGBRb\"\u003e100μM FIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus4cUvm3fv3n402VOiDg4k3b\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusMm3eQixfIPyHQzhVDBOfvc\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusOydioGZAJ0MRlE7SmarlOb\"\u003e100μM BIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusSKNpOTVj6FlhjlX59EDQVB\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusIIMX5kMC3xCO5Qzwavvhvf\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxushWqiDhGmWBINlUS48bbUcf\"\u003e100μM Loop F\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusLEFqMVjTU8J4Z6yJhixWzJ\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusl2UUS5gVArO4LfFTdTjHig\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuswGYGZZ03DxmoxuUs05PXzf\"\u003e100μM Loop B\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusgfjRQeCeySQZDwzxutwM1b\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusGdVbHUW78FYuv6RYzjJZ7f\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHpqWpWoaqhKatTgPBnimTc\"\u003e100μM F3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxushAD4aDyxnLm2RM5PSQJ8FJ\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusFDQBsm6Fh0i38oRVeK5lUg\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus0DKxYG0XVJ3MJW0YBxWUBe\"\u003e100μM B3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuseAXir6MQ4OU259tBEGAfXc\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusotvt8YluukfUT8nuq81tQd\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHHqAF65Shjh5OXFk4bHhCb\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusI0ZrX2Oqhwi5454BoToEHc\"\u003e24μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusUe7fi36LqNwJgLWNsBfUrg\"\u003e-\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusCpfdUpdENpR759EzhYWd4d\"\u003e\n\u003cdiv\u003eThaw all components to be used at room temperature. Vortex briefly 4×Fluorescent and Colorimetric LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex Bst DNA polymerase 2.0.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxuseI30Wyu1Zw4MsQoDBJt0uc\"\u003e\n\u003cdiv\u003eWhen using the kit for the first time，add all Bst DNA polymerase 2.0 to 4×Fluorescent and Colorimetric LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusuQgb0CUj4Fot84W1VpvxVh\"\u003e\n\u003cdiv\u003eFor a purification number of N+2, set up N+4 LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for LAMP Positive Control (PC) and another for LAMP Negative Control (NC) .\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv\u003e\n\u003ctable data-ace-table-col-widths=\"182;182;182;182\" class=\"ace-table\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusDRb6SYThNcCdBfTaDFKbFg\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusn3B21C6wutDYlHMAZtzb6d\"\u003ePC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2Tf3IqlvIQNnDRKgaewOod\"\u003eNC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus8iLiBZUHC4O68F5nVLloWh\"\u003eN+2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusxxvlpsNYptkc8Az988Qbgb\"\u003e4×Fluorescent and Colorimetric LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus4kA8dizJj4IYEpOxc2LOkd\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusU5hv7giO2tKB0dom3yxxHU\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusDqaTIB1K1F6hypabCaOAEb\"\u003e5μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusVVojrXOkTuO0Q4Q8UQda1c\"\u003e20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszYfBsbNtyP6Lq0H5C83onb\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusfKwvZtJbFZ6pKg01qDSWBF\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusROvKnbZqwmYqV5rH4vKAgm\"\u003e1μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusftyj86o8yZichZp7dU45le\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusfIOIkO3il6dKRE3dVpezZg\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusvHbxNyFmPQQ9azUuOVCbMc\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJihNz7yXR39bvWhHmVPVCe\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuseX0YYbGimQBdGDxp7FFDTe\"\u003eN+2 samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxussDc5CUigVzXocHYxae5Mhe\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusENeyiwMeZQOA1wkTqOelkd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusNT8LBrufw9cAvB4mmaJqEg\"\u003e1-14μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusqCmwxpp4nJ0xDh38LUWEEg\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusId0uaTH37PRuOPyvCGNc7b\"\u003e9μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus8pGRm3ypsh0iwnvkfYFVuh\"\u003e14μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus9S7A6p3woO9uDkKFCvOyDg\"\u003eAdd to 20μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusEWDFEjpE13aoHwwp2IWK0e\"\u003e\n\u003cdiv\u003eSeal reaction vessels.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusFPeb4dU92H3KRUu97F2Raf\"\u003e\n\u003cdiv\u003eIncubate at 65°C for 60 minutes in a real-time PCR machine. Collect signal in the SYBR channel at every 60 seconds.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusevg52Qrh8ScXP0W3d7LbXc\"\u003e\n\u003cdiv\u003eAfter the reaction is finished, remove tubes or vessels from incubation and examine by eye. The two positive reactions should have S-shaped fluorescent signal, the reaction mixtures will turn green. The two negative controls should have no S-shaped fluorescent signal and the reaction mixtures should turn light green.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"4\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusfkwmT9mHBmZ5MVBq4gzzjc\"\u003e\n\u003cdiv\u003eIf there is amplification in NC or there is no amplification in PC，the whole experiment is invalid. There is no need to analysis the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"5\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusLyGKLkUsmPnnYVGn3UIrWg\"\u003e\n\u003cdiv\u003eIf there is no amplification in NC or there is amplification in PC，the whole experiment is valid. Analyze and record he results of the N+2 samples. The results from fluorescent detection can be downloaded from the real-time PCR machine, the colorimetric results can be photographed or scanned for later use.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxussIb31DmbQewZGzhYMcTMTd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxussIb31DmbQewZGzhYMcTMTd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxussIb31DmbQewZGzhYMcTMTd\"\u003e\u003cstrong\u003eTroubleshooting\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus1AtcRxAI2lFxFTt5G2qG3e\"\u003e\n\u003cdiv\u003eThere is amplification in NC.\u003c\/div\u003e\n\u003col class=\"list-number2\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusJaHjMHwhMBVSkctMFdsfee\"\u003e\n\u003cdiv\u003eThis result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusVRT3HgrNc9RH50PiAAk7rw\"\u003e\n\u003cdiv\u003eThis result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C -70°C，or put the reaction tubes to 65°C as soon as the reaction is set，or use a new set of primer for the targeted DNA region.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxussujOQLiipdhaGe23EoIoGd\"\u003e\n\u003cdiv\u003eThere is no amplification in PC\u003c\/div\u003e\n\u003col class=\"list-number2\"\u003e\n\u003cli data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusB3e2cgXD2TGNX65dd5jxTr\"\u003e\n\u003cdiv\u003eThis result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusDTirNc57WcDj9tPxEdaa1f\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusDTirNc57WcDj9tPxEdaa1f\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusDTirNc57WcDj9tPxEdaa1f\"\u003e\u003cstrong\u003eLimited Product Warranty\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusIKpReo0XgFfnau65bmRWIB\"\u003e\n\u003cdiv\u003eBiofargo，Inc. warrants its products as set forth in the Biofargo’s General Terms and Conditions of Sale at www.Biofargo.com. If you have any questions, please contact Biofargo @ www.Biofargo.com.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusXUKePWtxnj1fymkIfEb0mg\"\u003e\n\u003cdiv\u003eThe information in this guide is subject to change without notice.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusBHzB6PplZftVmObOr7EiUf\"\u003e\n\u003cdiv\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, Biofargo INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":42196035240117,"sku":"211223-50","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/lamp_1eb0e19b-26aa-47d6-98b4-f4eb3969b6b8.png?v=1732872587"},{"product_id":"fluorescent-lamp-kit","title":"Fluorescent LAMP Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv data-page-id=\"doxuslH18Mou3isNE8VRRndcHoh\" data-docx-has-block-data=\"true\" data-mce-fragment=\"1\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusslrk5MTxUcqJ0F86rhTf9j\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusvvc3xhCXKFptDeawZIsGOf\" data-mce-fragment=\"1\"\u003eBiofargo Fluorescent LAMP Kit is designed to provide a one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA and an examination of the amplification results with real-time fluorescent detectors like real-time PCR machines. It has the following features：\u003c\/div\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusJz8F2syAlUmGfnsqp28Eqh\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eOptimized LAMP mix, contains a fluorescent dye.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusOhfhNzWdqty6ZMviq4lfSe\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eReal-time monitoring of the LAMP reactions using real-time PCR machine.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus1kvPViLxC2OGyLbG4BTeCb\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eUseful in optimizing LAMP primers or reaction parameters.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusiLujIS4UddVGaOajKwxKYb\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e4× concentration LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusAJ6Q0x0DWTJTB6p0C6zZRb\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than PCR.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusEtqNXqFFt8sNWN2PKRLQeg\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus9ulBoT8mqO1g15raMny5Ze\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eTemplate-primer mix supplied as LAMP positive control, eliminates false negative results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusCXtYlD8rds37A5lZfBSBCd\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eCAT#211222-50 is sufficient for 50 reactions of 20μL volume, and CAT#211222-500 for 500 reactions of 20μL volume.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusBEqKZxeh7FaGsP0KAjVOKh\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusBEqKZxeh7FaGsP0KAjVOKh\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInput Sample Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus5dMKnFYDLBeICzGpv5Muog\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform DNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications，one is for positive control and another negative control.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxus40aI0djGdgTHC9PYgqxlSf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxus40aI0djGdgTHC9PYgqxlSf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"243;243;243\" data-mce-fragment=\"1\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusIlq1X6oSqj1CRnrxJbrY0Y\" data-mce-fragment=\"1\"\u003eComponent\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuskKIRMOpwKnvnmTGJRpjv1f\" data-mce-fragment=\"1\"\u003eCAT#:211222-50\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusV0K7pWqdEwFpacN4zeiybG\" data-mce-fragment=\"1\"\u003eCAT#:211222-500\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusdgYm7lFp776e3Q91iSBYSc\" data-mce-fragment=\"1\"\u003e4×Fluorescent LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus4O3vczOhCBl5iTUDi8RwNc\" data-mce-fragment=\"1\"\u003e200μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxushecoIjyEhEX9iFnLZ7IIce\" data-mce-fragment=\"1\"\u003e1mL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusGguI79BCRPLprPiAnrtmRb\" data-mce-fragment=\"1\"\u003eBst DNA Polymerase 2.0\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusRRLk7smjJvaasHTbDbbYIb\" data-mce-fragment=\"1\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus4fcbRZEPeQ5dONWB91itUd\" data-mce-fragment=\"1\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusou9J6zbbvJqZsZcizM0GOf\" data-mce-fragment=\"1\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus53CmOYgQLJ1yVxIKjM02vf\" data-mce-fragment=\"1\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusAPk8b6LZDooglYmmcZxyHh\" data-mce-fragment=\"1\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxustfVTDkkOJ3yy75MidtTurg\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxustfVTDkkOJ3yy75MidtTurg\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxustfVTDkkOJ3yy75MidtTurg\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Handling\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus5Gm7vhtKBOqs0T4tTmYLQb\" data-mce-fragment=\"1\"\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusUgT5aNd36HlOvhOtAXGznB\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusUgT5aNd36HlOvhOtAXGznB\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusUgT5aNd36HlOvhOtAXGznB\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eRequired Materials not Supplied\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"365;365\" data-mce-fragment=\"1\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol width=\"365\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"365\" data-mce-fragment=\"1\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszqBvVnFVJmHcqTUW1piZJc\" data-mce-fragment=\"1\"\u003eNo\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusaaDZJvQKhVHIrhNJXEgZXe\" data-mce-fragment=\"1\"\u003eItem\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusYiB0ZxHquD7Bbv5aGyQhU9\" data-mce-fragment=\"1\"\u003e1\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus1Ht0QrycxX1FbuODglwMsd\" data-mce-fragment=\"1\"\u003ePrepared DNA Samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuss7LyhuI2kTPqA8zwnfEkDc\" data-mce-fragment=\"1\"\u003e2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusnE00spbiMG8cOiGYCYAyuc\" data-mce-fragment=\"1\"\u003eLAMP primers targeting DNA samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusPyPwk2dK3pyXn6FcFJrH2d\" data-mce-fragment=\"1\"\u003e3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusxAhlVnii8fes3siG1Yv1He\" data-mce-fragment=\"1\"\u003eReaction vessels compatible with instrument for LAMP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus8gmKaGih0Q9VyZGL2t0MUc\" data-mce-fragment=\"1\"\u003e4\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusLTEsvZArogGpMTyoPGfjjg\" data-mce-fragment=\"1\"\u003eMolecular biology grade ddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2edMGX3eEaFa70VoiL8EBc\" data-mce-fragment=\"1\"\u003e5\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusxg3sIQyF1bYL3nyEpRfsAg\" data-mce-fragment=\"1\"\u003eReal-time PCR machine\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2dvdtII2AkecUMF8zpzRpf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxus2dvdtII2AkecUMF8zpzRpf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxus2dvdtII2AkecUMF8zpzRpf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProcedural Guidelines\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusLqOjpgEY6PHcbbCT61nCNb\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusjk1N6GbSlyr2sw3F57xvrf\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusx0oT6dYjGs72Ta3lmcsjVd\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eAvoid opening reaction vessels after a LAMP reaction is completed..\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus7rIZGTMwnCxzgMmnTHwe8c\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eScreening at least 10 sets of LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusGkNDKXOHEGP74UjJFGLoHh\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eUse Loop primers if possible.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus8x2Kl1yhk8xxcWVxv948Rf\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePrimers should be HPLC purified.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusNpeKxz56065v6RYhBsSwlf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusNpeKxz56065v6RYhBsSwlf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProcedures\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col start=\"1\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxuslYvDtWs9O39NvEbdZTTlzf\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e Make 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified LAMP primers at 100μM each. Use the 100μM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100μL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"243;243;243\" data-mce-fragment=\"1\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"243\" data-mce-fragment=\"1\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxussZEpXFcfbajRCHUyBRH4qe\" data-mce-fragment=\"1\"\u003eComponents\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusi2yoPs0RwVoK3XyLd440Bf\" data-mce-fragment=\"1\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxussrzIWbUDSthQdDllQ6i94e\" data-mce-fragment=\"1\"\u003eVolume\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuseh3zaupp4DybJpT3bRGNWd\" data-mce-fragment=\"1\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusXsmHeip08eEOpdqzMWTWfh\" data-mce-fragment=\"1\"\u003eFinal Concentration\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszQJGzV8o0oXUd2dhe828lh\" data-mce-fragment=\"1\"\u003ein 20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJit4f3faQnNAfK1rceNlqf\" data-mce-fragment=\"1\"\u003e100μM FIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusTxUw4aiOWKNFfrhS5tKGjs\" data-mce-fragment=\"1\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusSQhglhSpSyVQnIePQIJdQg\" data-mce-fragment=\"1\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusRdJ5NfGjNmUbHjH80aKleg\" data-mce-fragment=\"1\"\u003e100μM BIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszvxIHZSZVydwMiw5ci6nrh\" data-mce-fragment=\"1\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus28KazLlzl3Cyu68nMp2lme\" data-mce-fragment=\"1\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusamozd1XImwx2eaPNb91OV8\" data-mce-fragment=\"1\"\u003e100μM Loop F\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus65hLM86cLzTcJ4d6cAxdZc\" data-mce-fragment=\"1\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusI13552Iymb3XRGi17EIDsg\" data-mce-fragment=\"1\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusv9jVivpo3sG1tOhPuRAtQd\" data-mce-fragment=\"1\"\u003e100μM Loop B\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusjUgM2sXfwZWfsFjiBZI3ob\" data-mce-fragment=\"1\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusMbS0mWt1jF5fZNpTTUetnv\" data-mce-fragment=\"1\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusL3nnxtggHeEUYojZTIfcad\" data-mce-fragment=\"1\"\u003e100μM F3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusauftvHoffJEK17BMLUI0Pc\" data-mce-fragment=\"1\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusjFxh72u5aGBO3gfs1wO4Hg\" data-mce-fragment=\"1\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusyCv57xsBMV1d7AOh10Whpe\" data-mce-fragment=\"1\"\u003e100μM B3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusLMLzezIT7Wj5eSujKTwE4c\" data-mce-fragment=\"1\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusZcEcqNMpuiMJK1oBql5WYb\" data-mce-fragment=\"1\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus1jERzGG8RCGgx8ufer64Ye\" data-mce-fragment=\"1\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusbmfhvowyffElBFGmeZ2gng\" data-mce-fragment=\"1\"\u003e24μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuswjvgsg654btdpEot9ALLuc\" data-mce-fragment=\"1\"\u003e-\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col start=\"1\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxussMj1fFG0kntqIKoD9yjZ6b\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThaw all components to be used at room temperature. Vortex briefly 4×Fluorescent LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex Bst DNA polymerase 2.0.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusOG9WHSH5LaZWl5qtagZZlc\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eWhen using the kit for the first time，add all Bst DNA polymerase 2.0 to 4×Fluorescent LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"3\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusPqiH4tTaBVSs31CGEsbaKc\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eFor a purification number of N+2, set up N+4 LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for LAMP Positive Control (PC) and another for LAMP Negative Control (NC) .\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"182;182;182;182\" data-mce-fragment=\"1\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol width=\"182\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"182\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"182\" data-mce-fragment=\"1\"\u003e \u003ccol width=\"182\" data-mce-fragment=\"1\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusc0EjrML1qdmA78zix1IHZg\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusmLkUbFvwiY2KX1anEycNu0\" data-mce-fragment=\"1\"\u003ePC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusT27Tca2QocgFiMNQlWmAsc\" data-mce-fragment=\"1\"\u003eNC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJZXshiBK8IuSjMJYlBdCRg\" data-mce-fragment=\"1\"\u003eN+2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusm1FbdtY4PHNGzVQWm8uQth\" data-mce-fragment=\"1\"\u003e4×Fluorescent LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHLN1uiYxp4L24qQs9Ovfvf\" data-mce-fragment=\"1\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusn8OMqTXMzHurexmYt86rkd\" data-mce-fragment=\"1\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxustrWlxoWyyFZyMmDDfpW1Ef\" data-mce-fragment=\"1\"\u003e5μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusA6YfbrKW1LnqwfbuDPqp1e\" data-mce-fragment=\"1\"\u003e20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusWZzr5mZIeGymDjP4mHkmEf\" data-mce-fragment=\"1\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusBblt7NotDaUi94ykfDtZVG\" data-mce-fragment=\"1\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus1a1EkiUZg1DVrZ9Unu560g\" data-mce-fragment=\"1\"\u003e1μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxush5E0XXsDQN2W1ZBf0iUfLb\" data-mce-fragment=\"1\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusKKzbpZGIqN2IiUflyR51Jc\" data-mce-fragment=\"1\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusd4YDoWEfb6fCyoIO1Izx5e\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusdzQrXqQ7XjMwmNEcW9wVKb\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusvZV6bxbJxpfmfvuU3IvOhc\" data-mce-fragment=\"1\"\u003eN+2 samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusEfPYddyWUtHNwN4RYU0DId\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusO0N9NnRVn3ZV8F0iz9gSVf\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusTvgKHvlobWn71R2kmm5VUd\" data-mce-fragment=\"1\"\u003e1-14μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus5FZJX9FE6TaXzsOvBpdXRC\" data-mce-fragment=\"1\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusBA5P8TVqGH1FqAWtJXGXae\" data-mce-fragment=\"1\"\u003e9μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJfsnuMyaFu3JGz1iGYb0oc\" data-mce-fragment=\"1\"\u003e14μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusPQaVHR8XCrOFDXFV8VLiAd\" data-mce-fragment=\"1\"\u003eAdd to 20μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col start=\"1\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusGnF9AR6Jq4yh9uN2xLmTDb\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eSeal reaction vessels.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusFzlVfoZIzxo7RJpecbnjNd\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIncubate at 65°C for 60 minutes in a real-time PCR machine. Collect signal in the SYBR channel in every 60 seconds.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"3\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusfqsFrq1fmI6WOfEeuUM8te\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThe two positive reactions should have S-shaped fluorescent signal. The two negative controls should have no S-shaped fluorescent signal.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"4\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusbw7TxNaTt7opsZBhWcWhY9\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is amplification in NC or there is no amplification in PC，the whole experiment is invalid. There is no need to analysis the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"5\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusQ9BCmdj5Xym5jiGEUAQztc\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is no amplification in NC or there is amplification in PC，the whole experiment is valid. Analyze and record he results of the N+2 samples. The results from fluorescent detection can be downloaded from the real-time PCR machine.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxust832TGR1c97oVf5WZC3yod\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxust832TGR1c97oVf5WZC3yod\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eTroubleshooting\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col start=\"1\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusvPWJ6A4Vav7EpbkesDlzYc\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is amplification in NC.\u003c\/div\u003e\n\u003col class=\"list-number2\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusThyEAKYAlJ8amTvRs41uDg\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusFclokEhRkosLDcIvW30taf\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C -70°C，or put the reaction tubes to 65°C as soon as the reaction is set，or use a new set of primer for the targeted DNA region.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus06qtss6v7q0HFzzr4b8Dmb\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is no amplification in PC\u003c\/div\u003e\n\u003col class=\"list-number2\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusEeMoYMZFovvLJYwwOT1G4f\" data-list=\"number\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusJ5rBsXDS6rYp0YRSUgDhLf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusJ5rBsXDS6rYp0YRSUgDhLf\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLimited Product Warranty\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusGREQK416jklwz0pUJOjNhh\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eBiofargo ，Inc. warrants its products as set forth in the Biofargo ’s General Terms and Conditions of Sale at www.biofargo usa.com. If you have any questions, please contact Biofargo @ www.Biofargo .com.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxuszTM4xAHtjHXI8vuhL3ssHd\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThe information in this guide is subject to change without notice.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\" data-mce-fragment=\"1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusBlJ7LpTGCWLjA7swV5iiIh\" data-list=\"bullet\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, Biofargo INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cspan data-lark-record-data='{\"isCut\":false,\"rootId\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"parentId\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"blockIds\":[7,8,9,10,11,12,13,14,15,16,17,18,19,20,45,46,47,48,73,74,75,76,77,78,79,80,81,82,107,108,109,110,135,136,137,138,139,140,141,144,146,147,148,149],\"recordIds\":[\"doxusslrk5MTxUcqJ0F86rhTf9j\",\"doxusvvc3xhCXKFptDeawZIsGOf\",\"doxusJz8F2syAlUmGfnsqp28Eqh\",\"doxusOhfhNzWdqty6ZMviq4lfSe\",\"doxus1kvPViLxC2OGyLbG4BTeCb\",\"doxusiLujIS4UddVGaOajKwxKYb\",\"doxusAJ6Q0x0DWTJTB6p0C6zZRb\",\"doxusEtqNXqFFt8sNWN2PKRLQeg\",\"doxus9ulBoT8mqO1g15raMny5Ze\",\"doxusCXtYlD8rds37A5lZfBSBCd\",\"doxusBEqKZxeh7FaGsP0KAjVOKh\",\"doxus5dMKnFYDLBeICzGpv5Muog\",\"doxus40aI0djGdgTHC9PYgqxlSf\",\"doxuskuXljJGQnza2MemFlbbDWc\",\"doxustfVTDkkOJ3yy75MidtTurg\",\"doxus5Gm7vhtKBOqs0T4tTmYLQb\",\"doxusUgT5aNd36HlOvhOtAXGznB\",\"doxus95JqfJOtMO1ioxwQ1KyFdn\",\"doxus2dvdtII2AkecUMF8zpzRpf\",\"doxusLqOjpgEY6PHcbbCT61nCNb\",\"doxusjk1N6GbSlyr2sw3F57xvrf\",\"doxusx0oT6dYjGs72Ta3lmcsjVd\",\"doxus7rIZGTMwnCxzgMmnTHwe8c\",\"doxusGkNDKXOHEGP74UjJFGLoHh\",\"doxus8x2Kl1yhk8xxcWVxv948Rf\",\"doxusNpeKxz56065v6RYhBsSwlf\",\"doxuslYvDtWs9O39NvEbdZTTlzf\",\"doxuscNrjAG9JwlzgLfNYngTKUl\",\"doxussMj1fFG0kntqIKoD9yjZ6b\",\"doxusOG9WHSH5LaZWl5qtagZZlc\",\"doxusPqiH4tTaBVSs31CGEsbaKc\",\"doxus0l8hzZhMCFeMmHwjKHV4ye\",\"doxusGnF9AR6Jq4yh9uN2xLmTDb\",\"doxusFzlVfoZIzxo7RJpecbnjNd\",\"doxusfqsFrq1fmI6WOfEeuUM8te\",\"doxusbw7TxNaTt7opsZBhWcWhY9\",\"doxusQ9BCmdj5Xym5jiGEUAQztc\",\"doxust832TGR1c97oVf5WZC3yod\",\"doxusvPWJ6A4Vav7EpbkesDlzYc\",\"doxus06qtss6v7q0HFzzr4b8Dmb\",\"doxusJ5rBsXDS6rYp0YRSUgDhLf\",\"doxusGREQK416jklwz0pUJOjNhh\",\"doxuszTM4xAHtjHXI8vuhL3ssHd\",\"doxusBlJ7LpTGCWLjA7swV5iiIh\"],\"recordMap\":{\"doxusslrk5MTxUcqJ0F86rhTf9j\":{\"id\":\"doxusslrk5MTxUcqJ0F86rhTf9j\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Product Description\"},\"attribs\":{\"0\":\"*1*0+j\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"7097835687914766341\"]},\"nextNum\":2}},\"type\":\"text\"}},\"doxusvvc3xhCXKFptDeawZIsGOf\":{\"id\":\"doxusvvc3xhCXKFptDeawZIsGOf\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Biofargo  Fluorescent LAMP Kit is designed to provide a one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA and an examination of the amplification results with real-time fluorescent detectors like real-time PCR machines. It has the following features：\"},\"attribs\":{\"0\":\"*0+7n\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]},\"nextNum\":1}},\"type\":\"text\"}},\"doxusJz8F2syAlUmGfnsqp28Eqh\":{\"id\":\"doxusJz8F2syAlUmGfnsqp28Eqh\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+1b\"},\"text\":{\"0\":\"Optimized LAMP mix, contains a fluorescent dye.\"}}},\"type\":\"bullet\"}},\"doxusOhfhNzWdqty6ZMviq4lfSe\":{\"id\":\"doxusOhfhNzWdqty6ZMviq4lfSe\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+1z\"},\"text\":{\"0\":\"Real-time monitoring of the LAMP reactions using real-time PCR machine.\"}}},\"type\":\"bullet\"}},\"doxus1kvPViLxC2OGyLbG4BTeCb\":{\"id\":\"doxus1kvPViLxC2OGyLbG4BTeCb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+1m\"},\"text\":{\"0\":\"Useful in optimizing LAMP primers or reaction parameters. \"}}},\"type\":\"bullet\"}},\"doxusiLujIS4UddVGaOajKwxKYb\":{\"id\":\"doxusiLujIS4UddVGaOajKwxKYb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+3s\"},\"text\":{\"0\":\"4× concentration LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\"}}},\"type\":\"bullet\"}},\"doxusAJ6Q0x0DWTJTB6p0C6zZRb\":{\"id\":\"doxusAJ6Q0x0DWTJTB6p0C6zZRb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2f\"},\"text\":{\"0\":\"High amplification efficiency, generally an order of magnitude more sensitive than PCR.\"}}},\"type\":\"bullet\"}},\"doxusEtqNXqFFt8sNWN2PKRLQeg\":{\"id\":\"doxusEtqNXqFFt8sNWN2PKRLQeg\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2g\"},\"text\":{\"0\":\"Isothermal amplification around 65°C, no need for expensive thermal cycling instruments.\"}}},\"type\":\"bullet\"}},\"doxus9ulBoT8mqO1g15raMny5Ze\":{\"id\":\"doxus9ulBoT8mqO1g15raMny5Ze\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2h\"},\"text\":{\"0\":\"Template-primer mix supplied as LAMP positive control, eliminates false negative results.\"}}},\"type\":\"bullet\"}},\"doxusCXtYlD8rds37A5lZfBSBCd\":{\"id\":\"doxusCXtYlD8rds37A5lZfBSBCd\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+35\"},\"text\":{\"0\":\"CAT#211222-50 is sufficient for 50 reactions of 20μL volume, and CAT#211222-500 for 500 reactions of 20μL volume.\"}}},\"type\":\"bullet\"}},\"doxusBEqKZxeh7FaGsP0KAjVOKh\":{\"id\":\"doxusBEqKZxeh7FaGsP0KAjVOKh\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":2,\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0*1+p\"},\"text\":{\"0\":\"Input Sample Requirements\"}}},\"type\":\"text\"}},\"doxus5dMKnFYDLBeICzGpv5Muog\":{\"id\":\"doxus5dMKnFYDLBeICzGpv5Muog\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxuslH18Mou3isNE8VRRndcHoh\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+5w\"},\"text\":{\"0\":\"Perform DNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. 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data-lark-record-format=\"docx\/record\" class=\"lark-record-clipboard\" data-mce-fragment=\"1\"\u003e\u003c\/span\u003e","brand":"wytsci","offers":[{"title":"Default Title","offer_id":42196156514485,"sku":"211222-50","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_pcrkit.webp?v=1751468806"},{"product_id":"colorimetric-lamp-kit","title":"Colorimetric LAMP Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv data-mce-fragment=\"1\" data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusmOX4qR2Jp9YYRkkaggcYmd\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusyE2ahKMPKbMZLpcWiIGlBb\"\u003eBingene Colorimetric LAMP Kit is designed to provide a one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA and an equipment-free examination of the amplification results with naked eyes. It has the following features：\u003c\/div\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusjjPjJ8ASYwep55bAyQNA3e\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eOptimized LAMP mix, contains colorimetric dyes that enables the examination of LAMP results with naked eyes.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusW1livffz2v571ResD6VrCe\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e4× concentration LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusvBGuqtsvN5hDSSHv0mdfbb\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than PCR.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusf6D649acsLpUGkQEk91Mnd\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusFBWSfOT6mywuqbtfbWtHPf\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eTemplate-primer mix supplied as LAMP positive control, eliminates false negative results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusI7j2QC63I9BaPMe0WDXP9e\" data-list=\"bullet\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eCAT#211221-50 is sufficient for 50 reactions of 20μL volume, and CAT#211221-500 for 500 reactions of 20μL volume.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv data-docx-has-block-data=\"false\" data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusrlZUz0VTN3jV8Ji0RdbrLd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusrlZUz0VTN3jV8Ji0RdbrLd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusrlZUz0VTN3jV8Ji0RdbrLd\"\u003e\u003cstrong\u003eInput Sample Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusHeQH5Lb63H2qiqJXC0pZrf\"\u003e\n\u003cdiv\u003ePerform DNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications，one is for positive control and another negative control.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv data-docx-has-block-data=\"false\" data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusxq7xXZU9zkLqHLJWaI63sd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusxq7xXZU9zkLqHLJWaI63sd\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusxq7xXZU9zkLqHLJWaI63sd\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-docx-has-block-data=\"true\" data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\"\u003e\n\u003cdiv\u003e\n\u003ctable data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusmFTfs0nL8zCssokkDwtWYc\"\u003eComponent\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusBsWiF0lDe3BtomXudhfoyd\"\u003eCAT#:211221-50\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2hmZYMrC3fHyEW7YHUWnEe\"\u003eCAT#:211221-500\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusOgeRpfNIRSvUOzPYL0gzqc\"\u003e4×Colorimetric LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusl06jWlHMhHWvbnxL6k3Wjf\"\u003e200μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxustBsJcPtNIezbqkFyVJTFoS\"\u003e1mL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusfBWYOJUafeANemWBavH0Wn\"\u003eBst DNA Polymerase 2.0\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusFG5mHf0M68UZZbNDDWt8td\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusxW2THjsFWTICDgCwLYVvHd\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusMzXbXfjYdKIbhFWo0Nr0ye\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusEEpZHCfxwQbHCSVMiUjKhf\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus5INCdQlAuAWg0DWI3xq6kc\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cdiv data-docx-has-block-data=\"false\" data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusFfbTMLI5TpXkqVAa2vUdWf\"\u003e\u003cstrong\u003eStorage and Handling\u003c\/strong\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJYShStmjnFBNQKshRHm7af\"\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxuskJQGkkvp6InHHUy6KUbFiR\"\u003e\u003cstrong\u003eRequired Materials not Supplied\u003c\/strong\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"true\"\u003e\n\u003cdiv\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"365;365\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"365\"\u003e \u003ccol width=\"365\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusn23QUC8WoupaJI0Z3Mhs6c\"\u003eNo\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusgdpVA1VNtFTuN9qRzyv4Zd\"\u003eItem\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusdDqRlcF8PHSacBXTX0wvNc\"\u003e1\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHPLhuFWAC0V0B4PoxKvaME\"\u003ePrepared DNA Samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusSwBbXgxN8Vx15C14dqnHcf\"\u003e2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusb6noMDgmr305Cjm15zEQlc\"\u003eLAMP primers targeting DNA samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus5qKtrT9T6ZCnK3NoyS8Mye\"\u003e3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusTcIbbmh0tskMGHYn9bseHg\"\u003eReaction vessels compatible with instrument for LAMP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusSEdxyOJZy0ISxUQ44S7End\"\u003e4\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusDxcNmIkDlO2tPI0J1i8Cog\"\u003eMolecular biology grade ddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxustqo4IRq4Zl8LdDrRpmst1g\"\u003e5\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusGWL0T22G0e4xbFeDUZpdgf\"\u003eParaffin oil, molecular biology grade\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuso1JL9tE0I8PuegT74kqd0b\"\u003e6\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus9coz5KCeGn2ZwTuuwUbQLh\"\u003eHeat block, water bath or PCR machine\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszs0X6nS2ffumXYVVmYY7Mf\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxus95EwXj6q5cbXwfP8MH7jee\"\u003e\u003cstrong\u003eProcedural Guidelines\u003c\/strong\u003e\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusLUd1lVKy24vmNvbYPvUEBF\" data-list=\"bullet\"\u003e\n\u003cdiv\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxuscOV7g1kvDNGzuScbDXTjod\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusteBNidQSDafVkwmUmimDwe\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eAvoid opening reaction vessels after a LAMP reaction is completed. But if agarose gel electrophoresis or other post-reaction analysis is desired, perform the analysis in a separated room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusJAMjXBIQZlyJee73m7o65f\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eScreening at least 10 sets of LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusY5BcZaJ1eka1GB8nI0M6Sc\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eUse Loop primers if possible.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusP6bB9CcEXUm78kgLWqN2WS\" data-list=\"bullet\"\u003e\n\u003cdiv\u003ePrimers should be HPLC purified.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\n\u003cstrong\u003eProcedures\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003col start=\"1\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusc5IHJVe45VAEH3kppJh0QU\" data-list=\"number\"\u003e\n\u003cdiv\u003e Make 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified LAMP primers at 100μM each. Use the 100μM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100μL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"true\"\u003e\n\u003cdiv\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"243;243;243\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003ccol width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuso9vVkhwyVdDnCNUDTYnsbd\"\u003eComponents\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszXcp9YF6VJ1BfJnJRpj2fh\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusA34dxzWsmpIsdHHPy62QJd\"\u003eVolume\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusIMRT8a52I1sivuYBLvI1mh\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusZL96kzPBHQUgdbcbrCGSKg\"\u003eFinal Concentration\u003c\/div\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus6uQ4MYuyuippoWlWq1N2Uc\"\u003ein 20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusw67ZtMhYZz2Ua80MTHzhab\"\u003e100μM FIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus73GhvAoTnf2fxnpDD4OSyc\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusssTeYlkCcBeWXXHzetDcRf\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusirzZ5t3xPysC9mNeLKxytr\"\u003e100μM BIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusdc8aKMVXWHTNzIMNPpWFFe\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus8ofJ5Eeutyi8Yr9y9UJmB3\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuspaxufDoyiVzxna2LcyIWdb\"\u003e100μM Loop F\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusIxK0wOMhNBul8GAwpfUEVc\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusIBXJMZf1EuKmRKhfHFZ0J2\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusADq5gXbLLoBsi1qCJGz12e\"\u003e100μM Loop B\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusoWWnLnbmKwpeRhZQ7G2Z9c\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusFx0zo8eUfdoYXfRJz087oc\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusssHmvAINYZ7FySgvY7nuye\"\u003e100μM F3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus7Hq2BdmmiokEdFFJLA5dxb\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusHqU1w35rOYCOTSt7AHsw8d\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusWTJfbBwxxDMDe2597oeoZg\"\u003e100μM B3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusnGNDCUxvqoYcK6ldUIZrgg\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusV6Ct6H9h6doSJaOT7yYnub\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusryvh8D0No4O7HGSHNzZgKc\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusq6G05lLYUXvUH8y7mKBUPZ\"\u003e24μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusuVRSW8ueSxbV4QUFQU9E1c\"\u003e-\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"true\"\u003e\n\u003col start=\"1\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusBPIyQRLcyGYFo5bOCQ5PBd\" data-list=\"number\"\u003e\n\u003cdiv\u003eThaw all components to be used at room temperature. Vortex briefly 4×Colorimetric LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex Bst DNA polymerase 2.0.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxust7JNb9Xcuchn1tydzTZAki\" data-list=\"number\"\u003e\n\u003cdiv\u003eWhen using the kit for the first time, add all Bst DNA polymerase 2.0 to 4×Colorimetric LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"3\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusfjaPyxmQmx1IaXz7o5rZve\" data-list=\"number\"\u003e\n\u003cdiv\u003eFor a purification number of N+2, set up N+4 LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for LAMP Positive Control (PC) and another for LAMP Negative Control (NC) .\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv\u003e\n\u003ctable class=\"ace-table\" data-ace-table-col-widths=\"182;182;182;182\"\u003e\n\u003ccolgroup\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003ccol width=\"182\"\u003e \u003c\/colgroup\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusYSRpWDT07mGIX2BlxWH8NA\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxush3LYzPt1DrXUJvdCgw1Wuf\"\u003ePC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusdu8n0ppmzSjZftktepqRCc\"\u003eNC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusCp3TnQQEIun0VhcJr4Ikuf\"\u003eN+2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusT6130wVM7CXqn4I847c70K\"\u003e4×Colorimetric LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusKx4nZR8ZmXC2JUA8Li4SKc\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxustU9OzyIKPnHyLbssqfJnYd\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusH2yjtYrVLC86PQ6QjwG4Tg\"\u003e5μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusmng5lIT6JCSPyfENNY34lh\"\u003e20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusl0BszLmyYhiX7NuQgdjHBg\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszU7m00fe0InW5vIHOfHUjc\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus3nEHykmLA4xqgddo5lOJic\"\u003e1μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusKxPXMVcVqa1OxySWNGgv38\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusjwDNR3NXLBg93eHb8wRttd\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusNlUlb25SyA1eoMHQ4BtkNf\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusgzQAZOMevY2G0XVJ19iSJf\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuszMtfmsGzSjXPcq7CGxleCg\"\u003eN+2 samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusfaxUh2UrCvmyTZHpLLEheb\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxuswInSkuJtydMNBjy5z0Y3Pg\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus5gOQJX9zz0WndQTUKOf7qd\"\u003e1-14μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus2EMD4V3QyOMa66mKiChkJe\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusQe6FRMQ6SUSKQvrPvwsMEh\"\u003e9μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxusJs5M7pTelO4tIIVfCo2UKe\"\u003e14μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\"\u003e\n\u003cdiv class=\"ace-line ace-line old-record-id-doxus4aBXW8lepdzid753lZCHBc\"\u003eAdd to 20μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col start=\"1\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusDWepaqInwS4clBHbTaR1Yb\" data-list=\"number\"\u003e\n\u003cdiv\u003eAdd 30μL paraffin oil if heat block, water bath is used as incubator. If a PCR machine is used as incubator, turn on the heat lid.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus6pFS3LyuJPRCkGkcIpxNee\" data-list=\"number\"\u003e\n\u003cdiv\u003eSeal reaction vessels.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"3\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusrArxDnf5Bpb3dewq0HmJeY\" data-list=\"number\"\u003e\n\u003cdiv\u003eIncubate at 65°C for 60 minutes.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"4\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusOWVGrKPkrq0rJZ8loR5hKc\" data-list=\"number\"\u003e\n\u003cdiv\u003eRemove tubes or vessels from incubation and examine by eye. Positive reactions will have turned green while negative controls should turn light green. If color difference is not obvious, allow reaction to cool at room temperature.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"5\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxuszOkXUGRqwcILU2k0wc0DSg\" data-list=\"number\"\u003e\n\u003cdiv\u003eIf there is amplification in NC or there is no amplification in PC，the whole experiment is invalid. There is no need to analysis the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"6\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusReFnbtdJiUdxpJLQNfzTtb\" data-list=\"number\"\u003e\n\u003cdiv\u003eIf there is no amplification in NC or there is amplification in PC，the whole experiment is valid. Analyze and record he results of the N+2 samples. The result can be photographed or scanned to record the colorimetric results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxusXZy0a3oCPpZBumHsabz6Eg\"\u003e\u003cstrong\u003eTroubleshooting\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col start=\"1\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus0VpsjBlh87qPtdKQwiCEKf\" data-list=\"number\"\u003e\n\u003cdiv\u003eThere is amplification in NC.\u003c\/div\u003e\n\u003col class=\"list-number2\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxussyk0BZ07OgDOKFCfk6ohkd\" data-list=\"number\"\u003e\n\u003cdiv\u003eThis result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusWLJPxlxfEDHrHHxxn9orhe\" data-list=\"number\"\u003e\n\u003cdiv\u003eThis result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C -70°C，put the reaction tubes to 65°C as soon as the reaction is set，use a new set of primer for the targeted DNA region.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col start=\"2\" class=\"list-number1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxus5hybG8yaFXYVO7AMYZ2XRc\" data-list=\"number\"\u003e\n\u003cdiv\u003eThere is no amplification in PC\u003c\/div\u003e\n\u003col class=\"list-number2\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusmc9S8tMQN4tSAWE9laDXmg\" data-list=\"number\"\u003e\n\u003cdiv\u003eThis result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv data-page-id=\"doxusCawjSmJ2zHQYkdlkSqd31i\" data-docx-has-block-data=\"false\"\u003e\n\u003ch2 class=\"ace-line ace-line old-record-id-doxustfUcyyvWDrkxg7H3tSyX7f\"\u003e\u003cstrong\u003eLimited Product Warranty\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusvWlaxmVzHDiQ7altAN8ifo\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eBingene，Inc. warrants its products as set forth in the Bingene’s General Terms and Conditions of Sale at www.tiandzusa.com. If you have any questions, please contact Bingene @ www.bingene.com.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusGGl2SOOCHVTUqqdnPErDog\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eThe information in this guide is subject to change without notice.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\" class=\"list-bullet1\"\u003e\n\u003cli class=\"ace-line ace-line old-record-id-doxusjiKFtw3wfFackFR6xLpV3e\" data-list=\"bullet\"\u003e\n\u003cdiv\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, BINGENE INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. \u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\" data-lark-record-data='{\"isCut\":false,\"rootId\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"parentId\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"blockIds\":[7,8,9,10,11,12,13,14],\"recordIds\":[\"doxusmOX4qR2Jp9YYRkkaggcYmd\",\"doxusyE2ahKMPKbMZLpcWiIGlBb\",\"doxusjjPjJ8ASYwep55bAyQNA3e\",\"doxusW1livffz2v571ResD6VrCe\",\"doxusvBGuqtsvN5hDSSHv0mdfbb\",\"doxusf6D649acsLpUGkQEk91Mnd\",\"doxusFBWSfOT6mywuqbtfbWtHPf\",\"doxusI7j2QC63I9BaPMe0WDXP9e\"],\"recordMap\":{\"doxusmOX4qR2Jp9YYRkkaggcYmd\":{\"id\":\"doxusmOX4qR2Jp9YYRkkaggcYmd\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Product Description\"},\"attribs\":{\"0\":\"*1*0+j\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"7097835687914766341\"]},\"nextNum\":2}},\"type\":\"text\"}},\"doxusyE2ahKMPKbMZLpcWiIGlBb\":{\"id\":\"doxusyE2ahKMPKbMZLpcWiIGlBb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+6o\"},\"text\":{\"0\":\"Bingene Colorimetric LAMP Kit is designed to provide a one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA and an equipment-free examination of the amplification results with naked eyes. It has the following features：\"}}},\"type\":\"text\"}},\"doxusjjPjJ8ASYwep55bAyQNA3e\":{\"id\":\"doxusjjPjJ8ASYwep55bAyQNA3e\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+30\"},\"text\":{\"0\":\"Optimized LAMP mix, contains colorimetric dyes that enables the examination of LAMP results with naked eyes.\"}}},\"type\":\"bullet\"}},\"doxusW1livffz2v571ResD6VrCe\":{\"id\":\"doxusW1livffz2v571ResD6VrCe\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+3s\"},\"text\":{\"0\":\"4× concentration LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\"}}},\"type\":\"bullet\"}},\"doxusvBGuqtsvN5hDSSHv0mdfbb\":{\"id\":\"doxusvBGuqtsvN5hDSSHv0mdfbb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2f\"},\"text\":{\"0\":\"High amplification efficiency, generally an order of magnitude more sensitive than PCR.\"}}},\"type\":\"bullet\"}},\"doxusf6D649acsLpUGkQEk91Mnd\":{\"id\":\"doxusf6D649acsLpUGkQEk91Mnd\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2g\"},\"text\":{\"0\":\"Isothermal amplification around 65°C, no need for expensive thermal cycling instruments.\"}}},\"type\":\"bullet\"}},\"doxusFBWSfOT6mywuqbtfbWtHPf\":{\"id\":\"doxusFBWSfOT6mywuqbtfbWtHPf\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2h\"},\"text\":{\"0\":\"Template-primer mix supplied as LAMP positive control, eliminates false negative results.\"}}},\"type\":\"bullet\"}},\"doxusI7j2QC63I9BaPMe0WDXP9e\":{\"id\":\"doxusI7j2QC63I9BaPMe0WDXP9e\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"CAT#211221-50 is sufficient for 50 reactions of 20μL volume, and CAT#211221-500 for 500 reactions of 20μL volume.\"},\"attribs\":{\"0\":\"*0+35\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]},\"nextNum\":1}},\"type\":\"bullet\"}},\"doxusCawjSmJ2zHQYkdlkSqd31i\":{\"id\":\"doxusCawjSmJ2zHQYkdlkSqd31i\",\"snapshot\":{\"align\":\"\",\"author\":\"7097835687914766341\",\"children\":[\"doxusdGXbii6Js9qMooLrEibtPd\",\"doxusojMA7OP5jvz2nV8WZ62LGb\",\"doxusmOX4qR2Jp9YYRkkaggcYmd\",\"doxusyE2ahKMPKbMZLpcWiIGlBb\",\"doxusjjPjJ8ASYwep55bAyQNA3e\",\"doxusW1livffz2v571ResD6VrCe\",\"doxusvBGuqtsvN5hDSSHv0mdfbb\",\"doxusf6D649acsLpUGkQEk91Mnd\",\"doxusFBWSfOT6mywuqbtfbWtHPf\",\"doxusI7j2QC63I9BaPMe0WDXP9e\",\"doxusrlZUz0VTN3jV8Ji0RdbrLd\",\"doxusHeQH5Lb63H2qiqJXC0pZrf\",\"doxusxq7xXZU9zkLqHLJWaI63sd\",\"doxusL9ZrAr2hLnV9yDlS4kCxIe\",\"doxusFfbTMLI5TpXkqVAa2vUdWf\",\"doxusJYShStmjnFBNQKshRHm7af\",\"doxuskJQGkkvp6InHHUy6KUbFiR\",\"doxusmhsgexDGF15nB30utQhQIf\",\"doxuszs0X6nS2ffumXYVVmYY7Mf\",\"doxus95EwXj6q5cbXwfP8MH7jee\",\"doxusLUd1lVKy24vmNvbYPvUEBF\",\"doxuscOV7g1kvDNGzuScbDXTjod\",\"doxusteBNidQSDafVkwmUmimDwe\",\"doxusJAMjXBIQZlyJee73m7o65f\",\"doxusY5BcZaJ1eka1GB8nI0M6Sc\",\"doxusP6bB9CcEXUm78kgLWqN2WS\",\"doxusItA8ptZgqEOoaWvJ744F6H\",\"doxusc5IHJVe45VAEH3kppJh0QU\",\"doxusSMjsx5HpOgwl4892ucfYrg\",\"doxusBPIyQRLcyGYFo5bOCQ5PBd\",\"doxust7JNb9Xcuchn1tydzTZAki\",\"doxusfjaPyxmQmx1IaXz7o5rZve\",\"doxusC4ehEq0AbeHogsCd0CVs9c\",\"doxusDWepaqInwS4clBHbTaR1Yb\",\"doxus6pFS3LyuJPRCkGkcIpxNee\",\"doxusrArxDnf5Bpb3dewq0HmJeY\",\"doxusOWVGrKPkrq0rJZ8loR5hKc\",\"doxuszOkXUGRqwcILU2k0wc0DSg\",\"doxusReFnbtdJiUdxpJLQNfzTtb\",\"doxusXZy0a3oCPpZBumHsabz6Eg\",\"doxus0VpsjBlh87qPtdKQwiCEKf\",\"doxus5hybG8yaFXYVO7AMYZ2XRc\",\"doxustfUcyyvWDrkxg7H3tSyX7f\",\"doxusvWlaxmVzHDiQ7altAN8ifo\",\"doxusGGl2SOOCHVTUqqdnPErDog\",\"doxusjiKFtw3wfFackFR6xLpV3e\",\"doxusADv5qA28C4xLwQYnD5jzxe\",\"doxusU08PehbrGjrnpQFu8UeyRe\",\"doxusOpSuAqTmZhufdzXHoaPs0b\"],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+w\"},\"text\":{\"0\":\"b4e3fbfabb1318a1db799547faed0638\"}}},\"type\":\"page\"}}},\"payloadMap\":{\"doxusmOX4qR2Jp9YYRkkaggcYmd\":{\"level\":1},\"doxusyE2ahKMPKbMZLpcWiIGlBb\":{\"level\":1}},\"extra\":{\"mention_page_title\":{},\"external_mention_url\":{}},\"pasteFlag\":\"8eb768c9-8b76-4c3c-85d9-4715845ae2a8\"}' data-lark-record-format=\"docx\/record\" class=\"lark-record-clipboard\"\u003e\u003c\/span\u003e","brand":"wytsci","offers":[{"title":"Default Title","offer_id":42196212056245,"sku":"211221-50","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/lamp_67d112bd-847a-47aa-9130-3f1234b13647.png?v=1732868987"},{"product_id":"fluorescent-rt-lamp-kit","title":"Colorimetric RT-LAMP Kit","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003cdiv data-mce-fragment=\"1\" data-docx-has-block-data=\"true\" data-page-id=\"doxusm0NcmkRWh0XN8PEKBgToue\"\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus9JJjHf9NPJdLgj20ISzZCd\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Description\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6ywSwdq9jKQLCCigk3MPsg\"\u003eBiofargo Colorimetric RT-LAMP Kit is designed to provide a one-step solution for Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) of RNA and an equipment-free examination of the amplification results with naked eyes. It has the following features：\u003c\/div\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusBoTrOumaTEU5qFRQ379Lrb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eOptimized RT-LAMP mix, contains colorimetric dyes that enables the examination of RT-LAMP results with naked eyes.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusrzxImMgEW34befv8g1lm58\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e4× concentration RT-LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxuscF06Pt2lFLNHStA5GA9737\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than RT-PCR.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusX5lpJVuOZU0vNhHTNp6BSh\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus72dqnXfTWa0KCk1NgwYO1e\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eTemplate-primer mix supplied as RT-LAMP positive control, eliminates false negative results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusuIl3NKsTy8WZIza4ox2kQD\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eCAT#211209-50 is sufficient for 50 reactions of 20μL volume, and CAT#211209-500 for 500 reactions of 20μL volume.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2XbNbM42AGxcsOC7OOdTXg\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2XbNbM42AGxcsOC7OOdTXg\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eInput Sample Requirements\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxuszWZIrZr5yNgYnNXwKZw4Ze\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform RNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications，one is for positive control and another negative control.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYYh4jE9T9h1a7lkWj9So8f\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYYh4jE9T9h1a7lkWj9So8f\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus8wL0RNSQ8n4vkTOYsube5c\"\u003eComponent\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusG9V3NdbiVKw6qOkvoEkv5f\"\u003eCAT#:211209-50\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusdkB23wiQCk20PHvCsGNtXc\"\u003eCAT#:211209-500\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuszOXjrKhnHJFQx5K9yzpL3b\"\u003eColorimetric RT-LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusPACY2Ta1ytQtBj7Zwf0jSb\"\u003e200μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusCfOuCb0iQPrGTmaoMuRh4g\"\u003e1mL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYpE4bhHmk2VyzqL39J602d\"\u003eRTase-Bst Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6tFsGdzcpoOzMVAeLyrVfb\"\u003e100μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusloCQMuERVg61L7yu2ieuGd\"\u003e500μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusaAIuZHu8wfUnLzwJfBw9Fb\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuscRsxy5kyMKhydfZkgA2FOe\"\u003e50μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusSkoyOOdGHEGCVqMgQBNPbp\"\u003e250μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Handling\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusGbEj36lJZzy48Tb4cLCsgc\"\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusGbEj36lJZzy48Tb4cLCsgc\"\u003e\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMOICCQsmE1R7DV4d2cwEVh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMOICCQsmE1R7DV4d2cwEVh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusMOICCQsmE1R7DV4d2cwEVh\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eRequired Materials not Supplied\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"365;365\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"365\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"365\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusahwPUa2E5lnKX9D4pQZ9dd\"\u003eNo\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus6BPvvWdiC4gHpEB5JkucZe\"\u003eItem\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusM1U1HV8IITy9u2ILpoAV5f\"\u003e1\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus4Yki3yWWZADw7UnI3t1gEb\"\u003ePrepared RNA Samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus474YRGXleIQ53K2aC6wLTb\"\u003e2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusnj4DZdlI6QAr4OvwIXDW9b\"\u003eRT-LAMP primers targeting RNA samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusJcqSBU0bFdezAJUmJbYuxe\"\u003e3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusmkS9bXPBUEyb8PUXAs2gXb\"\u003eReaction vessels compatible with instrument for RT-LAMP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusFbkNcwyRcV5eOwTlce4Sbh\"\u003e4\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusm4uluycgIIFzlljDQYoJBb\"\u003eMolecular biology grade ddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusR7uf17zWOrBvtJ7zt3gW5f\"\u003e5\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2zrwwMJv1xeKoeBjMzuLqh\"\u003eParaffin oil, molecular biology grade\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusfoIgwjXl2uvTPmnFEzfrgh\"\u003e6\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusoNKnpd1PqSy457K1wOI1Vg\"\u003eHeat block, water bath or PCR machine\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProcedural Guidelines\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusaTxxiTrNC5CCHBwiRBixUg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus8eXPSQddPgaq0scB4OPX8c\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusQDVr8qIgTFpy0kufTd2Rcg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eAvoid opening reaction vessels after a RT-LAMP reaction is completed. But if agarose gel electrophoresis or other post-reaction analysis is desired, perform the analysis in a separated room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus5cSm8DiVh0xmH4ESf9UcKc\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eScreening at least 10 sets of RT-LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus9RB5cj81gh1398348gdORe\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eUse Loop primers if possible.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus3nSzGIkjWgdCatFnoGp1pd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003ePrimers should be HPLC purified.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusKFM5PSlQIWbUGQ39J0ZnYd\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusKFM5PSlQIWbUGQ39J0ZnYd\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProcedures\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxustuwqZpXsM3iSbYjjZfO6Me\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e Make 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified RT-LAMP primers at 100μM each. Use the 100uM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100uL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"243;243;243\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"243\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxush4pn2IsvdaHEKvMokQxuGb\"\u003eComponents\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuszxlRR8zJQqPoS9u0p93YJg\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusoLrHHXfce68bEyp66Dtznh\"\u003eVolume\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusfQosxnwQUOIe1enUczM9bh\"\u003eto add\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusjbd2P1SbW91HbSt2ivyD0f\"\u003eFinal Concentration\u003c\/div\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuskOAVWjUlgCxt1UhBZHNdrd\"\u003ein 20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2cyL3fSrUFEpsCxbf3MfIf\"\u003e100μM FIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuskmPDmkVTVj4ncdigWVygzb\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusFhNv4xR5QRnRXYqic2fwAh\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusiTK4gm33uUsvrLzPrF3WDg\"\u003e100μM BIP\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusyfktPkszNjGYT3vYsJ2fjg\"\u003e32μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxushvKPzG6euxli4GTaUVbwof\"\u003e32 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuslrWO2dfL7LXlREv7RneDtf\"\u003e100μM Loop F\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusao53l5VYF2BYd0NUuH9eYd\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYZrc1dqADOSLcsGvpGxYUe\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusNQY4qpOlYgmZZ6ux6CBPUf\"\u003e100μM Loop B\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus2boDEZ2vq6QSUHTZYEaF7c\"\u003e4μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDkMUlQIVD2eZINpnK7U5Vc\"\u003e8 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusv6SjnZqr0FfAO4YGxMw5ad\"\u003e100μM F3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusXwFKdNhjKjF2h67qAD4tze\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusJkJ10Z34ZgfD77kkxKorib\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusL1xw9asRThbS7uaUmeU55g\"\u003e100μM B3\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuslNz0whlBM2qnDOTlb0Dvse\"\u003e2μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusWmvWoisfx5iCfcsnZKBjrg\"\u003e4 μM\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusVyS6A7QCPQK9WaLGBL8lyc\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusm3vxiA2hUbvUd2tud6hPAb\"\u003e24μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusifADiaJlFDKupX9UdmyL7b\"\u003e-\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusFw0YtBnKXc5nH29Dtap2jb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThaw all components to be used at room temperature. Vortex briefly Colorimetric RT-LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex RTase-Bst Mix.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus6bNBHVu0ydekLN2wZCXUvb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eWhen using the kit for the first time，add all RTase-Bst Mix to Colorimetric RT-LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusavJ2DwS2QPoI6Q8NUrgidb\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eFor a purification number of N+2, set up N+4 RT-LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for RT-LAMP Positive Control (PC) and another for RT-LAMP Negative Control (NC) .\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003ctable data-mce-fragment=\"1\" data-ace-table-col-widths=\"182;182;182;182\" class=\"ace-table\"\u003e\n\u003ccolgroup data-mce-fragment=\"1\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003ccol data-mce-fragment=\"1\" width=\"182\"\u003e \u003c\/colgroup\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus1LvRmBnAUmpAZMihQHfK2d\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxushmhrD8HohZmLEGkrwXKZXg\"\u003ePC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusnImTZUuTHqsIwCJlfI4306\"\u003eNC\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusGaLEwdPERgfKUU0zCJmk8c\"\u003eN+2\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusgW7uijJVjeBJV2ghPSFlwc\"\u003eColorimetric RT-LAMP Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDSEyXxdjykr9ZwFMj041Ch\"\u003e6μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusc5643thbYDTa6bXr5Zznme\"\u003e6μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusAkGpHWA8twxkCcajgz8PdG\"\u003e6μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxuswihdGt63LutC41bamVwUfT\"\u003e20×Primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusYkk2i1Z11nv1xCQaVwhfnb\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxussI3Y7hf2eAju8hZZKxviHd\"\u003e1μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusY73pRSWyZQWXBl9LR7gWLc\"\u003e1μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusf6Ril9j9i9qjmIWGXX5nvb\"\u003eTemplate-primer Mix\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusIDaN6km9qJ7h7MpX4h0FHe\"\u003e5μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusC0q2Xtbl0NAvOAbthW2YTh\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusUdLxsGhJCl9CGpMeORXTdg\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusVttKp2GbFGvyU3o3XX5JGg\"\u003eN+2 samples\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxus9stzOHU8G1eG8JF58Oxtpe\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusJ1Zf2MZepoRnonpGjRlLye\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusHsi8bTW6yxVRP1cv7ZksTX\"\u003e1-13μL each\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusRSsu5ZtEoBQ9k1IWPfYhph\"\u003eddH2O\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusegxDw94nFoz6ltfVcm3Q8f\"\u003e8μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusa9JbyLuOOdl4CNEv192ete\"\u003e13μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" rowspan=\"1\" colspan=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusSNSfkU6Rpt4aTYFUAVVtVc\"\u003eAdd to 20μL\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusK8Tq0IDScUKhhRwxTnbddd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eAdd 30uL paraffin oil if heat block, water bath is used as incubator. If a PCR machine is used as incubator, turn on the heat lid.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusmNDyUoFE7Et5bAQ0LdK7Ih\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eSeal reaction vessels.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"3\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusXr2t1itz34mDnYm2Rf5SUd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIncubate at 65°C for 60 minutes.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"4\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusBoQZ1ahwIrk6ct73Snfh3f\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eRemove tubes or vessels from incubation and examine by eye. Positive reactions will have turned green while negative controls should turn light green. If color difference is not obvious, allow reaction to cool at room temperature.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"5\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusqlOCIR1zDduCNNmTNkqk2g\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is amplification in NC or there is no amplification in PC，the whole experiment is invalid. There is no need to analysis the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"6\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus34LJUIKJ1WsK2pV42bXT2g\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eIf there is no amplification in NC or there is amplification in PC，the whole experiment is valid. Analyze and record he results of the N+2 samples. The result can be photographed or scanned to record the colorimetric results.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDR3IjF9Obmcr5JDgTZagMg\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusDR3IjF9Obmcr5JDgTZagMg\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eTroubleshooting\u003c\/strong\u003e\u003c\/h2\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxus5IUmBPcAhC3qjElwOOrnmf\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is amplification in NC.\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusV4MhgKmQIgsnlTvEBmr7Hg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-RT-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusKeIRaoqQrCLENm7VIkxnAd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C-70°C，put the reaction tubes to 65°C as soon as the reaction is set，use a new set of primer for the targeted DNA region.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number1\" start=\"2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusrmG4UN0LE2qGswxgGJxood\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThere is no amplification in PC\u003c\/div\u003e\n\u003col data-mce-fragment=\"1\" class=\"list-number2\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"number\" class=\"ace-line ace-line old-record-id-doxusc0FEcV5aD3cDVyniZUZIgd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThis result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusBihfFk9BX2ZUoqQOD9lIWc\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\" class=\"ace-line ace-line old-record-id-doxusBihfFk9BX2ZUoqQOD9lIWc\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLimited Product Warranty\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxusXp7D1VjsQ8SycLEOerQcZd\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eBiofargo ，Inc. warrants its products as set forth in the Biofargo ’s General Terms and Conditions of Sale at www.tiandzusa.com. If you have any questions, please contact Biofargo @ www.Biofargo .com.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxus5KWL5cD6mEAnyV8Uqq9A1e\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eThe information in this guide is subject to change without notice.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul data-mce-fragment=\"1\" class=\"list-bullet1\" start=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\" data-list=\"bullet\" class=\"ace-line ace-line old-record-id-doxuszlvwoPSw8le23xNqIbbLjg\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, Biofargo INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\" class=\"lark-record-clipboard\" data-lark-record-format=\"docx\/record\" data-lark-record-data='{\"isCut\":false,\"rootId\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"parentId\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"blockIds\":[7,8,9,10,11,12,13,14,15,16,17,18,43,44,45,46,75,76,77,78,79,80,81,82,83,84,136,137,138,139,188,189,190,191,192,193,194,195,198,200,201,202,203],\"recordIds\":[\"doxus9JJjHf9NPJdLgj20ISzZCd\",\"doxus6ywSwdq9jKQLCCigk3MPsg\",\"doxusBoTrOumaTEU5qFRQ379Lrb\",\"doxusrzxImMgEW34befv8g1lm58\",\"doxuscF06Pt2lFLNHStA5GA9737\",\"doxusX5lpJVuOZU0vNhHTNp6BSh\",\"doxus72dqnXfTWa0KCk1NgwYO1e\",\"doxusuIl3NKsTy8WZIza4ox2kQD\",\"doxus2XbNbM42AGxcsOC7OOdTXg\",\"doxuszWZIrZr5yNgYnNXwKZw4Ze\",\"doxusYYh4jE9T9h1a7lkWj9So8f\",\"doxusXQB3sqWq6911TMKfLr6pOc\",\"doxusTma8YgdJCwfjMrMzbgexDd\",\"doxusGbEj36lJZzy48Tb4cLCsgc\",\"doxusMOICCQsmE1R7DV4d2cwEVh\",\"doxushekVLPAwXUFAyHBPBcrSib\",\"doxus6tWHeIFWVfBLGfKiLjh6ug\",\"doxusaTxxiTrNC5CCHBwiRBixUg\",\"doxus8eXPSQddPgaq0scB4OPX8c\",\"doxusQDVr8qIgTFpy0kufTd2Rcg\",\"doxus5cSm8DiVh0xmH4ESf9UcKc\",\"doxus9RB5cj81gh1398348gdORe\",\"doxus3nSzGIkjWgdCatFnoGp1pd\",\"doxusKFM5PSlQIWbUGQ39J0ZnYd\",\"doxustuwqZpXsM3iSbYjjZfO6Me\",\"doxusCwn43kIZzRLslu0lyggkug\",\"doxusFw0YtBnKXc5nH29Dtap2jb\",\"doxus6bNBHVu0ydekLN2wZCXUvb\",\"doxusavJ2DwS2QPoI6Q8NUrgidb\",\"doxusezB9m9iATRiRKdyRvAHZsd\",\"doxusK8Tq0IDScUKhhRwxTnbddd\",\"doxusmNDyUoFE7Et5bAQ0LdK7Ih\",\"doxusXr2t1itz34mDnYm2Rf5SUd\",\"doxusBoQZ1ahwIrk6ct73Snfh3f\",\"doxusqlOCIR1zDduCNNmTNkqk2g\",\"doxus34LJUIKJ1WsK2pV42bXT2g\",\"doxusDR3IjF9Obmcr5JDgTZagMg\",\"doxus5IUmBPcAhC3qjElwOOrnmf\",\"doxusrmG4UN0LE2qGswxgGJxood\",\"doxusBihfFk9BX2ZUoqQOD9lIWc\",\"doxusXp7D1VjsQ8SycLEOerQcZd\",\"doxus5KWL5cD6mEAnyV8Uqq9A1e\",\"doxuszlvwoPSw8le23xNqIbbLjg\"],\"recordMap\":{\"doxus9JJjHf9NPJdLgj20ISzZCd\":{\"id\":\"doxus9JJjHf9NPJdLgj20ISzZCd\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Product Description\"},\"attribs\":{\"0\":\"*1*0+j\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"7097835687914766341\"]},\"nextNum\":2}},\"type\":\"text\"}},\"doxus6ywSwdq9jKQLCCigk3MPsg\":{\"id\":\"doxus6ywSwdq9jKQLCCigk3MPsg\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"initialAttributedTexts\":{\"text\":{\"0\":\"Biofargo  Colorimetric RT-LAMP Kit is designed to provide a one-step solution for Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) of RNA and an equipment-free examination of the amplification results with naked eyes. It has the following features：\"},\"attribs\":{\"0\":\"*0+7i\"}},\"apool\":{\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]},\"nextNum\":1}},\"type\":\"text\"}},\"doxusBoTrOumaTEU5qFRQ379Lrb\":{\"id\":\"doxusBoTrOumaTEU5qFRQ379Lrb\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+36\"},\"text\":{\"0\":\"Optimized RT-LAMP mix, contains colorimetric dyes that enables the examination of RT-LAMP results with naked eyes.\"}}},\"type\":\"bullet\"}},\"doxusrzxImMgEW34befv8g1lm58\":{\"id\":\"doxusrzxImMgEW34befv8g1lm58\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+3v\"},\"text\":{\"0\":\"4× concentration RT-LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\"}}},\"type\":\"bullet\"}},\"doxuscF06Pt2lFLNHStA5GA9737\":{\"id\":\"doxuscF06Pt2lFLNHStA5GA9737\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2i\"},\"text\":{\"0\":\"High amplification efficiency, generally an order of magnitude more sensitive than RT-PCR.\"}}},\"type\":\"bullet\"}},\"doxusX5lpJVuOZU0vNhHTNp6BSh\":{\"id\":\"doxusX5lpJVuOZU0vNhHTNp6BSh\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2g\"},\"text\":{\"0\":\"Isothermal amplification around 65°C, no need for expensive thermal cycling instruments.\"}}},\"type\":\"bullet\"}},\"doxus72dqnXfTWa0KCk1NgwYO1e\":{\"id\":\"doxus72dqnXfTWa0KCk1NgwYO1e\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+2k\"},\"text\":{\"0\":\"Template-primer mix supplied as RT-LAMP positive control, eliminates false negative results.\"}}},\"type\":\"bullet\"}},\"doxusuIl3NKsTy8WZIza4ox2kQD\":{\"id\":\"doxusuIl3NKsTy8WZIza4ox2kQD\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+35\"},\"text\":{\"0\":\"CAT#211209-50 is sufficient for 50 reactions of 20μL volume, and CAT#211209-500 for 500 reactions of 20μL volume.\"}}},\"type\":\"bullet\"}},\"doxus2XbNbM42AGxcsOC7OOdTXg\":{\"id\":\"doxus2XbNbM42AGxcsOC7OOdTXg\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":2,\"numToAttrib\":{\"0\":[\"bold\",\"true\"],\"1\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0*1+p\"},\"text\":{\"0\":\"Input Sample Requirements\"}}},\"type\":\"text\"}},\"doxuszWZIrZr5yNgYnNXwKZw4Ze\":{\"id\":\"doxuszWZIrZr5yNgYnNXwKZw4Ze\",\"snapshot\":{\"align\":\"left\",\"author\":\"7097835687914766341\",\"children\":[],\"comments\":null,\"hidden\":false,\"locked\":false,\"parent_id\":\"doxusm0NcmkRWh0XN8PEKBgToue\",\"text\":{\"apool\":{\"nextNum\":1,\"numToAttrib\":{\"0\":[\"author\",\"7097835687914766341\"]}},\"initialAttributedTexts\":{\"attribs\":{\"0\":\"*0+5w\"},\"text\":{\"0\":\"Perform RNA purification with a positive and a negative control. 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Title","offer_id":42196215398581,"sku":"211209-50","price":150.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_pcrkit.webp?v=1751468806"},{"product_id":"fluorescent-rt-lamp-kit-1","title":"Fluorescent RT-LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo Fluorescent RT-LAMP Kit is designed to provide a one-step solution for Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) of RNA and an examination of the amplification results with real-time fluorescent detectors like real-time PCR machines. It has the following features：\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOptimized RT-LAMP mix, contains a fluorescent dye.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReal-time monitoring of the RT-LAMP reactions using real-time PCR machine.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUseful in optimizing RT-LAMP primers or reaction parameters.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e4× concentration RT-LAMP mix allows larger in-put sample volumes than 2× concentration mix, which can increase the sensitivity of the test.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh amplification efficiency, generally an order of magnitude more sensitive than RT-PCR.\u003c\/p\u003e\n\u003cp\u003eIsothermal amplification around 65°C, no need for expensive thermal cycling instruments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTemplate-primer mix supplied as RT-LAMP positive control, eliminates false negative results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCAT#211210-50 is sufficient for 50 reactions of 20μL volume, and CAT#211210-500 for 500 reactions of 20μL volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInput Sample Requirements\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePerform DNA purification with a positive and a negative control. For a sample number of N, perform N+2 purifications. For the two additional purifications, one is for positive control and the other is for negative control.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003eCAT#:211210-50\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003eCAT#:211210-500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eFluorescent RT-LAMP Mix\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e200μL\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e1mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eRTase-Bst Mix\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e100μL\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e500μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eTemplate-primer Mix\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e50μL\u003c\/td\u003e\n\u003ctd style=\"width: 30%; text-align: center; vertical-align: middle;\"\u003e250μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage and Handling\u003c\/h2\u003e\n\u003cp\u003eStore the kit at -20°C. The kit is stable for at least one year from date of receipt.\u003c\/p\u003e\n\u003ch2\u003eRequired Materials not Supplied\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003eNo\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003eItem\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003ePrepared RNA Samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003eRT-LAMP primers targeting RNA samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003eReaction vessels compatible with instrument for RT-LAMP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003eMolecular biology grade ddH2O\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center; vertical-align: middle;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 85%; text-align: center; vertical-align: middle;\"\u003eReal-time PCR machine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProcedural Guidelines\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePerform all steps at room temperature (20–25°C) unless otherwise noted.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRegularly de-contaminate working areas and equipment using chlorine bleach to avoid potential carryover contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid opening reaction vessels after a RT-LAMP reaction is completed.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eScreening at least 10 sets of RT-LAMP primers for optimal sensitivity and specificity before choosing a final set.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUse Loop primers if possible.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrimers should be HPLC purified.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProcedures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eMake 20×Primer Mix. Use appropriate amount of ddH2O to dissolve the HPLC-purified RT-LAMP primers at 100μM each. Use the 100μM stocks to make 100μL 20×Primer Mix according to the following table. If volumes other than 100μL (50μL, 200μL etc.) is desired, adjust volumes accordingly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eComponents \u003cbr\u003eto add\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eVolume\u003cbr\u003eto add\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eFinal Concentration\u003cbr\u003ein 20×Primer Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM FIP\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e32μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e32 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM BIP\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e32μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e32 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM Loop F\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e4μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e8 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM Loop B\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e4μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e8 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM F3\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e4 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e100μM B3\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e4 μM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e24μL\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThaw all components to be used at room temperature. Vortex briefly Fluorescent RT-LAMP Mix and Template-primer Mix. Centrifuge to collect material and place on ice. Don’t vortex RTase-Bst Mix.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWhen using the kit for the first time，add all RTase-Bst Mix to Fluorescent RT-LAMP Mix and gently invert the tube for one minute to mix thoroughly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor a purification number of N+2, set up N+4 RT-LAMP reactions as described below. Volumes listed are for 20μL reaction. For the two additional reactions, one is for RT-LAMP Positive Control (PC) and the other is for RT-LAMP Negative Control (NC).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003ePC\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eNC\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eN+2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eFluorescent RT-LAMP Mix\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e6μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e6μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e6μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003e20×Primer Mix\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eTemplate-primer Mix\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e5μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eN+2 samples\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e1-13μL each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; text-align: center; vertical-align: middle;\"\u003eddH2O\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e8μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003e13μL\u003c\/td\u003e\n\u003ctd style=\"width: 20%; text-align: center; vertical-align: middle;\"\u003eAdd to 20μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e \u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e1. Seal reaction vessels.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e2. Incubate at 65°C for 60 minutes in a real-time PCR machine. Collect signal in the SYBR channel in every 60 seconds.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e3. The two positive reactions should have S-shaped fluorescent signal. The two negative controls should have no S-shaped fluorescent signal.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e4. If there is amplification in NC or there is no amplification in PC, the whole experiment is invalid. There is no need to analyze the results of the N+2 samples. Refer to Troubleshooting section.\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e5. If there is no amplification in NC or there is amplification in PC, the whole experiment is valid. Analyze and record the results of the N+2 samples. The results from fluorescent detection can be downloaded from the real-time PCR machine.\u003c\/p\u003e\n\u003ch2\u003eTroubleshooting\u003c\/h2\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003e1. There is amplification in NC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px;\"\u003e1. This result suggests possible carryover contamination in working environment and\/or reagents. To avoid carryover contamination, clean working areas and equipment using a 10% chlorine bleach solution, use new (un-contaminated) reagents, primer stocks, water, etc., use UNG-RT-LAMP Kit, perform experiments in a new (un-contaminated) room.\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px;\"\u003e2. This result also suggests nonspecific amplification. To avoid nonspecific amplification, do amplification at temperature of 65°C -70°C，or put the reaction tubes to 65°C as soon as the reaction is set，or use a new set of primer for the targeted DNA region.\u003cbr\u003e\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px;\"\u003e\u003cstrong\u003e2. There is no amplification in PC\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px;\"\u003e1. This result suggests failures in machine or reagents，use new machine or new set of reagents.\u003c\/p\u003e\n\u003ch2\u003eLimited Product Warranty\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBiofargo，Inc. warrants its products as set forth in the Biofargo’s General Terms and Conditions of Sale at www.biofargousa.com. If you have any questions, please contact Biofargo @ www.biofargo.com.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe information in this guide is subject to change without notice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDISCLAIMER: TO THE EXTENT ALLOWED BY LAW, BIOFARGO INC. WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":42197095481525,"sku":"211210-50","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/new_pcrkit.webp?v=1751468806"},{"product_id":"skygen-citrobacter-rodentium-colorimetric-and-fluorescent-lamp-kit","title":"Citrobacter rodentium Colorimetric and Fluorescent LAMP Kit","description":"\u003cstyle\u003e\n  \n  .josh-faq {\n    background: #ffffff;\n    color: #000000;\n    font-family: 'IBM Plex Sans', sans-serif;\n  }\n\n  .josh-faq,\n  .josh-faq * {\n    box-sizing: border-box;\n  }\n\n  .josh-faq__item {\n    border-bottom: 1px solid #e5e7eb;\n    padding: 0;\n  }\n\n  .josh-faq__item:first-child {\n    border-top: 1px solid #e5e7eb;\n  }\n\n  .josh-faq__question {\n    width: 100%;\n    background: none;\n    border: none;\n    text-align: left;\n    padding: 20px 0;\n    font-size: 16px;\n    font-weight: 700;\n    color: #000;\n    font-family: 'IBM Plex Sans', sans-serif;\n    cursor: pointer;\n    display: flex;\n    align-items: center;\n    justify-content: space-between;\n    gap: 16px;\n    line-height: 1.4;\n  }\n\n  .josh-faq__question:hover {\n    color: #055da9;\n  }\n\n  .josh-faq__icon {\n    flex-shrink: 0;\n    width: 20px;\n    height: 20px;\n    position: relative;\n    transition: transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before,\n  .josh-faq__icon::after {\n    content: '';\n    position: absolute;\n    background: currentColor;\n    border-radius: 2px;\n    transition: opacity 0.25s ease, transform 0.25s ease;\n  }\n\n  .josh-faq__icon::before {\n    width: 100%;\n    height: 2px;\n    top: 50%;\n    left: 0;\n    transform: translateY(-50%);\n  }\n\n  .josh-faq__icon::after {\n    width: 2px;\n    height: 100%;\n    left: 50%;\n    top: 0;\n    transform: translateX(-50%);\n  }\n\n  .josh-faq__item.is-open .josh-faq__icon::after {\n    opacity: 0;\n    transform: translateX(-50%) rotate(90deg);\n  }\n\n  .josh-faq__answer {\n    display: none;\n    padding: 0 0 20px 0;\n    font-size: 16px;\n    line-height: 1.6;\n    color: #333;\n    max-width: 860px;\n  }\n\n  .josh-faq__answer.is-open {\n    display: block;\n  }\n\n  .josh-faq__answer p {\n    margin: 0 0 15px;\n  }\n\n  .josh-faq__answer p:last-child {\n    margin-bottom: 0;\n  }\n\n  .josh-faq__answer ul {\n    margin: 8px 0 12px;\n    padding-left: 22px;\n  }\n\n  .josh-faq__answer li {\n    margin-bottom: 6px;\n    list-style-type: disc;\n  }\n\n  .josh-faq__answer a {\n    color: #055da9;\n    text-decoration: none;\n  }\n\n  .josh-faq__answer a:hover {\n    text-decoration: underline;\n  }\n\n  @media (max-width: 767px) {\n    .josh-faq__question {\n      font-size: 15px;\n      padding: 16px 0;\n    }\n\n    .josh-faq__answer {\n      font-size: 15px;\n    }\n  }\n\u003c\/style\u003e\r\n\r\n\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eCitrobacter rodentium Colorimetric and Fluorescent LAMP Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified DNA is directly amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification. The resulting signals may be used for comparative analysis of target nucleic acids.\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e Supplied without internal control. Internal control version can be customized based on your assay requirements.\u003cbr\u003eThis product was previously listed under catalog number 15-38720 and is now updated to BF-87897276 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eKey Features\u003c\/h2\u003e\r\n\r\n\u003cul\u003e\r\n  \u003cli data-end=\"191\" data-start=\"127\"\u003e\u003cp data-end=\"124\" data-start=\"62\"\u003eOne-step PCR workflow to reduce handling steps and minimize contamination risk.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli data-end=\"191\" data-start=\"127\"\u003e\r\n\u003cp\u003eDemonstrates high sensitivity under optimized laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"268\" data-start=\"194\"\u003e\r\n\u003cp\u003eHigh specificity based on primer-probe design.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"336\" data-start=\"271\"\u003e\r\n\u003cp\u003eIncludes a non-infectious plasmid-based positive control for assay verification.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"389\" data-start=\"339\"\u003e\r\n\u003cp\u003eCompatible with commonly used DNA purification methods.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"453\" data-start=\"392\"\u003e\r\n\u003cp\u003eSuitable for research applications including assay development and method evaluation.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"529\" data-start=\"456\"\u003e\r\n\u003cp\u003eBroad dynamic range under laboratory conditions.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli data-end=\"574\" data-start=\"532\"\u003e\r\n\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic use.\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eKit Components\u003c\/h2\u003e\r\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\r\n\u003ctbody\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDescription\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLid Color\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2×Probe qPCR Master Mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eContains optimized buffer and enzyme mix\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBlue\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePrimer-Probe Powder\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLyophilized target-specific primer and probe set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e50 T\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBrown\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePositive Control (1E7 copies\/μL)\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNon-infectious plasmid-based control\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYellow\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNuclease-free water \/ Dilution Buffer\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFor reaction setup\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 mL\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1.5 mL Vial\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGreen\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003ctr\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eManual\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eInstruction booklet\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1 Set\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003ctd style=\"width: 20%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNA\u003c\/td\u003e\r\n\u003c\/tr\u003e\r\n\u003c\/tbody\u003e\r\n\u003c\/table\u003e\r\n\u003cp\u003e \u003c\/p\u003e\r\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eStore at - 20 °C\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003cli\u003e\r\n\u003cp\u003eThe product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003ch2\u003eFrequently Asked Questions(FAQs)\u003c\/h2\u003e\r\n\u003cp\u003eMeet our leadership team, dedicated to bringing Biofargo's vision to life while supporting our customers, partners, and employees with excellence and integrity.\u003c\/p\u003e\r\n\r\n\u003cdiv class=\"josh-faq\"\u003e\r\n  \u003c!-- FAQ List --\u003e\r\n      \u003csection style=\"padding: 30px 300px 60px 30px;\"\u003e\r\n        \u003cdiv class=\"josh-faq__list\"\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is a ROX Dye included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo — ROX dye is not included with the kit. We can add a passive reference dye matched to your instrument upon request for $25 USD. To request a ROX dye, please contact us for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is an Internal Control included with the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eUnless otherwise specified, internal controls are not included in our standard kits, but are available as an add-on for all kits at a surcharge of $150 USD. To request a kit with an internal control, please reach out for a quote at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Is validation data available for the kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eValidation data may be available upon request, depending on the target. Standard validation generated from plasmid-based positive controls can typically be provided for many kits. Validation generated using the actual pathogen is handled case by case — it requires that we are able to source the pathogen, or that the customer supplies an extracted nucleic acid sample. Please note all kits are intended strictly for research use only. To enquire, contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              What is the lead time for all PCR kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eStandard production takes 7–14 days, after which kits ship from our laboratory to our warehouse. Expedited production (~7 days) is available upon request. We also maintain a ready inventory of our most popular kits to reduce wait times.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Can I customize this kit?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer custom assays. Customization can include target, pack size, reaction volume, master mix format, and inclusion of an internal control. Pricing is evaluated based on your specific customization requirements and quoted accordingly. For details, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Will your kits work with any PCR system?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eOur kits use standard FAM-based probe chemistry and are compatible with virtually all real-time PCR platforms. They are validated and optimized on widely used systems — including the Applied Biosystems QuantStudio series — and run reliably on other mainstream instruments using the same FAM detection channel. If you let us know your specific instrument model, we are happy to confirm the optimal channel and filter settings for your setup.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you offer discounts for bulk orders of kits?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we offer substantial discounts for bulk orders on most PCR kits. Discounts are available upon request through quotes only and do not apply to online orders. Bulk discounts apply to kits of the same type ordered at the same time, following this standard structure:\u003c\/p\u003e\r\n              \u003cul\u003e\r\n                \u003cli\u003e5% OFF (Qty. 5–9 kits)\u003c\/li\u003e\r\n                \u003cli\u003e10% OFF (Qty. 10–14 kits)\u003c\/li\u003e\r\n                \u003cli\u003e25% OFF (Qty. 15–29 kits)\u003c\/li\u003e\r\n                \u003cli\u003e35% OFF (Qty. 30+ kits)\u003c\/li\u003e\r\n              \u003c\/ul\u003e\r\n              \u003cp\u003eFor more information about discounted pricing, please contact us at \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits internationally?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eYes — we ship internationally via FedEx International Express (generally CIF or DAP terms). All international shipments include ~40 lbs of dry ice, sufficient for a maximum of 7 days in transit. We can also assist with customs clearance (completing paperwork and providing COAs, MSDS, HS codes, etc.). A dry ice surcharge applies: $15 USD for domestic shipments and $55 USD for international shipments.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Do you ship qPCR kits on ice packs or at room temperature?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. We do not ship qPCR kits on blue ice or at room temperature due to our strict quality requirements. This kit is shipped on dry ice. Kits may retain limited stability at room temperature, but should be stored at −20°C for best performance.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n          \u003cdiv class=\"josh-faq__item\"\u003e\r\n            \u003cbutton class=\"josh-faq__question\" aria-expanded=\"false\"\u003e\r\n              Are your kits approved for diagnostic or clinical use?\r\n              \u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\r\n            \u003c\/button\u003e\r\n            \u003cdiv class=\"josh-faq__answer\"\u003e\r\n              \u003cp\u003eNo. All Biofargo kits are strictly for research use only (RUO). Biofargo, Inc. is not authorized to sell products for diagnostic, detection, clinical, personal, or any other non-research application. Use of our kits outside of an authorized laboratory research context is not permitted.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n          \u003c\/div\u003e\r\n\r\n        \u003c\/div\u003e\r\n      \u003c\/section\u003e\r\n\r\n\u003c\/div\u003e\r\n\r\n\r\n\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/pdf\/15-38720.pdf\"\u003e\u003cstrong\u003eCitrobacter rodentium Colorimetric and Fluorescent LAMP Kit User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/biofargo\/msds\/15-38720.pdf\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\r\n\u003cscript\u003e\r\n(function () {\r\n  var items = document.querySelectorAll('.josh-faq__item');\r\n  items.forEach(function (item) {\r\n    var btn = item.querySelector('.josh-faq__question');\r\n    var answer = item.querySelector('.josh-faq__answer');\r\n    btn.addEventListener('click', function () {\r\n      var isOpen = item.classList.contains('is-open');\r\n      \/\/ Close all\r\n      items.forEach(function (el) {\r\n        el.classList.remove('is-open');\r\n        el.querySelector('.josh-faq__question').setAttribute('aria-expanded', 'false');\r\n        el.querySelector('.josh-faq__answer').classList.remove('is-open');\r\n      });\r\n      \/\/ Toggle clicked\r\n      if (!isOpen) {\r\n        item.classList.add('is-open');\r\n        btn.setAttribute('aria-expanded', 'true');\r\n        answer.classList.add('is-open');\r\n      }\r\n    });\r\n  });\r\n}());\r\n\u003c\/script\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282329067701,"sku":"BF-87897276","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_5eb198b2-f602-43a7-b370-41f3785b3f2e.jpg?v=1774190332"},{"product_id":"skygen-chlamydophila-psittaci-colorimetric-and-fluorescent-lamp-kit","title":"Chlamydophila psittaci Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eChlamydophila psittaci Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-11320 and is now updated to BF-5375958 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282461778101,"sku":"16-11320","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_8d44e7cb-90b8-484b-92b8-f0a905a6165f.png?v=1747142044"},{"product_id":"skygen-bos-mutus-ingredient-colorimetric-and-fluorescent-lamp-kit","title":"Bos mutus-Ingredient Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBos mutus-Ingredient Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-118 and is now updated to BF-7495197 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282461810869,"sku":"16-118","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_1ebd9305-703c-498f-8c04-3d50ed9931ca.png?v=1747142036"},{"product_id":"skygen-monkeypox-virus-colorimetric-and-fluorescent-lamp-kit","title":"Monkeypox Virus  Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMonkeypox Virus  Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-13600 and is now updated to BF-7686441 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462236853,"sku":"16-13600","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_f47a0816-2e2c-405e-8229-e6af124ea938.png?v=1747142027"},{"product_id":"skygen-monkeypox-virus-west-african-strain-colorimetric-fluorescent-lamp-kit","title":"Monkeypox Virus West African Strain Colorimetric-Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMonkeypox Virus West African Strain Colorimetric-Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-13601 and is now updated to BF-7572310 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462269621,"sku":"16-13601","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_2fa3a301-ad32-4f8e-901b-f2e98e6b1248.png?v=1747142017"},{"product_id":"skygen-monkeypox-virus-central-african-strain-colorimetric-fluorescent-lamp-kit","title":"Monkeypox Virus Central African Strain Colorimetric-Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMonkeypox Virus Central African Strain Colorimetric-Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-13602 and is now updated to BF-7058081 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462302389,"sku":"16-13602","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_9029e04e-0ec9-41bd-8fce-061c18d2e3c4.png?v=1747142006"},{"product_id":"skygen-rat-mitochondrial-dna-colorimetric-and-fluorescent-lamp-kit","title":"Rat Mitochondrial DNA Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eRat Mitochondrial DNA Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-142 and is now updated to BF-8512941 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462335157,"sku":"16-142","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_a8e79eb7-eabd-46d2-a77d-3c09ec4210e6.png?v=1747141999"},{"product_id":"skygen-borrelia-spp-colorimetric-and-fluorescent-lamp-kit","title":"Borrelia spp. Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBorrelia spp. Colorimetric and Fluorescent  LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-14400 and is now updated to BF-3162863 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462367925,"sku":"16-14400","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_e65c43ab-04a1-41f7-8477-cf36946a5e66.png?v=1747141989"},{"product_id":"skygen-streptococcus-gallolyticus-subsp-gallolyticus-colorimetric-and-fluorescent-lamp-kit","title":"Streptococcus gallolyticus subsp. gallolyticus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eStreptococcus gallolyticus subsp. gallolyticus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-18822 and is now updated to BF-6225891 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462400693,"sku":"16-18822","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_da26e758-a605-4a41-a015-373c505cda5a.png?v=1747141976"},{"product_id":"skygen-toxophasma-gondii-colorimetric-and-fluorescent-lamp-kit","title":"Toxophasma gondii Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eToxophasma gondii Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-21200 and is now updated to BF-1357056 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462433461,"sku":"16-21200","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_3058e06a-92d3-4cad-97a3-b396dc8dc2d5.png?v=1747141967"},{"product_id":"skygen-feline-parvovirus-colorimetric-and-fluorescent-lamp-kit","title":"Feline Parvovirus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eFeline Parvovirus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-21400 and is now updated to BF-1557164 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462466229,"sku":"16-21400","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_3ae241d9-ef1f-492b-ad6d-08c4866d6d0b.png?v=1747141958"},{"product_id":"skygen-african-swine-fever-virus-colorimetric-and-fluorescent-lamp-kit","title":"African Swine Fever Virus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eAfrican Swine Fever Virus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-23000 and is now updated to BF-2926311 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462531765,"sku":"16-23000","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_1bff0aad-8a99-4ed8-bda4-e109e9e6a0cf.png?v=1747141950"},{"product_id":"skygen-yersinia-pestis-colorimetric-and-fluorescent-lamp-kit","title":"Yersinia pestis Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eYersinia pestis Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-29420 and is now updated to BF-5861749 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462597301,"sku":"16-29420","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_a96d1d67-e145-4d05-8769-1484b1888baf.png?v=1747141942"},{"product_id":"skygen-polyomavirus-muris-1-colorimetric-and-fluorescent-lamp-kit","title":"Polyomavirus Muris 1 Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003ePolyomavirus Muris 1 Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-30713 and is now updated to BF-6634559 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462630069,"sku":"16-30713","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_bf6ace53-4052-400d-ad7e-a67ec3ddb504.png?v=1747141933"},{"product_id":"skygen-schistosoma-spp-colorimetric-and-fluorescent-lamp-kit","title":"Schistosoma spp. Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSchistosoma spp. Colorimetric and Fluorescent  LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32000 and is now updated to BF-9980620 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462695605,"sku":"16-32000","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_a9952156-e9e6-4323-b144-8467695d35e1.png?v=1747141925"},{"product_id":"skygen-staphylococcus-aureus-colorimetric-and-fluorescent-lamp-kit","title":"Staphylococcus aureus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eStaphylococcus aureus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32110 and is now updated to BF-8225866 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462761141,"sku":"16-32110","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_ffc0b408-a3d3-4880-940a-dccc61ada55e.png?v=1747141917"},{"product_id":"skygen-lactobacillus-delbrueckii-subsp-bulgaricus-colorimetric-and-fluorescent-lamp-kit","title":"Lactobacillus delbrueckii subsp. bulgaricus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eLactobacillus delbrueckii subsp. bulgaricus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32328 and is now updated to BF-1675752 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462793909,"sku":"16-32328","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_a84f6389-36f5-4d45-8168-cd3854e746cf.png?v=1747141908"},{"product_id":"skygen-babesia-spp-colorimetric-and-fluorescent-lamp-kit","title":"Babesia spp. Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBabesia spp. Colorimetric and Fluorescent  LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32400 and is now updated to BF-8815383 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462826677,"sku":"16-32400","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_d4baea20-852a-49f6-a97e-22657eba19bd.png?v=1747141896"},{"product_id":"skygen-babesia-bigemina-colorimetric-and-fluorescent-lamp-kit","title":"Babesia bigemina Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBabesia bigemina Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32410 and is now updated to BF-4505529 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462859445,"sku":"16-32410","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_d1380a60-2af5-4a46-9f4c-23bc756cbe7b.png?v=1747141887"},{"product_id":"skygen-salmonella-enterica-colorimetric-and-fluorescent-lamp-kit","title":"Salmonella enterica Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSalmonella enterica Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-32850 and is now updated to BF-2275831 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462892213,"sku":"16-32850","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_74653366-3a00-4641-85e1-2ed1cdffe7ea.png?v=1747141876"},{"product_id":"skygen-bifidobacterium-longum-colorimetric-and-fluorescent-lamp-kit","title":"Bifidobacterium longum Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBifidobacterium longum Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-33920 and is now updated to BF-2049659 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462924981,"sku":"16-33920","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_2a3a6e7d-b203-444d-99fb-ffaef71a4aa4.png?v=1747141867"},{"product_id":"skygen-veillonella-parvula-colorimetric-and-fluorescent-lamp-kit","title":"Veillonella parvula Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eVeillonella parvula Colorimetric and Fluorescent  LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-36850 and is now updated to BF-9143491 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462957749,"sku":"16-36850","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_d58f20e2-6f86-40fe-8eb3-03c8c1caacca.png?v=1747141857"},{"product_id":"skygen-hepatitis-b-virus-colorimetric-and-fluorescent-lamp-kit","title":"Hepatitis B Virus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eHepatitis B Virus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-37080 and is now updated to BF-8753097 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282462990517,"sku":"16-37080","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_a0de33a6-5c0a-45a1-9050-178636156a49.png?v=1747141849"},{"product_id":"skygen-gardnerella-vaginalis-colorimetric-and-fluorescent-lamp-kit","title":"Gardnerella vaginalis Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eGardnerella vaginalis Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-38100 and is now updated to BF-8614750 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463023285,"sku":"16-38100","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_80ad333e-0c5f-45ad-81c8-53d5d89a1f24.png?v=1747141838"},{"product_id":"skygen-escherichia-coli-colorimetric-and-fluorescent-lamp-kit","title":"Escherichia coli Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eEscherichia coli Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-39800 and is now updated to BF-9943619 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463056053,"sku":"16-39800","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_ecf74034-1c71-4d18-b30e-98ec9fb334af.png?v=1747141831"},{"product_id":"skygen-trichophyton-spp-colorimetric-and-fluorescent-lamp-kit","title":"Trichophyton spp. Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eTrichophyton spp. Colorimetric and Fluorescent  LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-44500 and is now updated to BF-7924620 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463088821,"sku":"16-44500","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_c860a4d5-0bc1-47d1-8a03-8211d88877d3.png?v=1747141824"},{"product_id":"skygen-gluten-ingredient-colorimetric-and-fluorescent-lamp-kit","title":"Gluten-Ingredient Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eGluten-Ingredient Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-505 and is now updated to BF-9702382 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463121589,"sku":"16-505","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_560f96df-7752-4a30-a809-d41ab0cbda76.png?v=1747141816"},{"product_id":"skygen-pyricularia-grisea-colorimetric-fluorescent-lamp-kit","title":"Pyricularia grisea Colorimetric-Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003ePyricularia grisea Colorimetric-Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-5185 and is now updated to BF-5521127 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463154357,"sku":"16-5185","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_c6f2836d-b980-494c-aa73-31478e614ae5.png?v=1747141808"},{"product_id":"skygen-spiroplasma-eriocheiris-colorimetric-and-fluorescent-lamp-kit","title":"Spiroplasma eriocheiris Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eSpiroplasma eriocheiris Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-59140 and is now updated to BF-2622847 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463187125,"sku":"16-59140","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_4b842a61-d41b-4ece-9705-184b39b02800.png?v=1747141798"},{"product_id":"skygen-trypanosoma-evansi-colorimetric-and-fluorescent-lamp-kit","title":"Trypanosoma evansi Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eTrypanosoma evansi Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-66070 and is now updated to BF-1463387 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463219893,"sku":"16-66070","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_396dddc5-a41e-4dfb-842b-d879b488935b.png?v=1747141787"},{"product_id":"skygen-mycoplasma-felis-colorimetric-and-fluorescent-lamp-kit","title":"Mycoplasma felis Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMycoplasma felis Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-74050 and is now updated to BF-7140327 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463252661,"sku":"16-74050","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_c2acecc6-5918-4d81-83aa-ff266b58fa9e.png?v=1747141779"},{"product_id":"skygen-mycoplasma-hyorhinis-colorimetric-and-fluorescent-lamp-kit","title":"Mycoplasma hyorhinis Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMycoplasma hyorhinis Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-74130 and is now updated to BF-3886154 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463285429,"sku":"16-74130","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_7062b34a-c102-4642-9d68-03fba3666c8b.png?v=1747141770"},{"product_id":"skygen-mycoplasma-pneumoniae-colorimetric-and-fluorescent-lamp-kit","title":"Mycoplasma pneumoniae Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eMycoplasma pneumoniae Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-74180 and is now updated to BF-7778772 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463318197,"sku":"16-74180","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_c6e68940-1489-4cde-8f3f-9debeaff3f72.png?v=1747141761"},{"product_id":"skygen-vancomycin-resistant-enterococcus-colorimetric-and-fluorescent-lamp-kit","title":"Vancomycin-Resistant Enterococcus Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eVancomycin-Resistant Enterococcus Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-81800 and is now updated to BF-9802145 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463350965,"sku":"16-81800","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_f4a29039-d225-41db-b709-ed92b67c5aa9.png?v=1747141753"},{"product_id":"skygen-bacillus-coagulans-colorimetric-and-fluorescent-lamp-kit","title":"Bacillus coagulans Colorimetric and Fluorescent LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\r\n\u003cp\u003eBacillus coagulans Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples. \u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 16-85600 and is now updated to BF-2676226 for improved product management.\u003c\/p\u003e\r\n\u003ch2\u003eFeatures\u003c\/h2\u003e\r\n\u003cul\u003e\r\n\u003cli\u003e\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003cli\u003e\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\u003c\/li\u003e\r\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":45282463383733,"sku":"16-85600","price":470.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_45694be0-6ce2-4782-a3fb-ed1c3940f6ac.png?v=1747141280"},{"product_id":"gmo-potato-rbmt15-101-colorimetric-and-fluorescent-lamp-kit","title":"GMO Potato RBMT15-101 Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eGMO Potato RBMT15-101 Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 12-GMO2330 and is now updated to BF-8556387 for improved product management.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244534923445,"sku":"12-GMO2330","price":440.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_1f1d0811-1aad-418a-8cb5-034dfca0ea49.png?v=1750507640"},{"product_id":"gmo-potato-semt15-102-colorimetric-and-fluorescent-lamp-kit","title":"GMO Potato SEMT15-102 Colorimetric and Fluorescent  LAMP Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eGMO Potato SEMT15-102 Colorimetric and Fluorescent LAMP Kit is designed for the quantitative pathogen screening kits of its specific DNA in a real-time PCR test using hydrolysis probes. In the amplification process, Taq DNA polymerase amplifies the DNA sample via quantitative PCR using a primer-probe set that targets its specific DNA region. At each cycle of the PCR, the 5' nuclease activity of Taq DNA polymerase degrades the bound probes, releasing the quenched specific FAM signal. The increase in FAM fluorescence measures the target gene-specific DNA amplifications. At a point where the fluorescence signal is confidently detected over the background signal, a Ct value can be determined for the FAM signal. This Ct parameter can be used to evaluate relative target abundance among samples.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e This product was previously listed under catalog number 12-GMO2340 and is now updated to BF-7658770 for improved product management.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOne-tube PCR, minimized carry-over contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh sensitivity, with an analytical LOD at 100 copies\/reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh specificity, no cross-reaction with DNA from other pathogens.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAmplification positive control is provided in the kit.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInstrument compatible: ABI, Bio-Rad, Roche, QuantStudio, etc..\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for both qualitative and quantitative pathogen screening kits. For quantitative use, the linear range is at least 5 orders of magnitude.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEnough for 50 reactions, 20 μL per reaction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"SkyGen","offers":[{"title":"Default Title","offer_id":51244535152821,"sku":"12-GMO2340","price":440.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_2500_c6880def-95cd-41eb-95e8-66b3d92c421c.png?v=1750507650"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/LAMP_kits.png?v=1741712844","url":"https:\/\/biofargo.com\/collections\/lamp-kits.oembed?page=6","provider":"Biofargo","version":"1.0","type":"link"}