{"title":"Innate \u0026 Adaptive Immunity","description":"","products":[{"product_id":"hla-a-02-01","title":"HLA-A*02:01","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eSerological Name:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eA2\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003ePeptide Load:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eEndogenous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eSize:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e30.0 µg\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eModification:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eUnmodified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eVolume:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e30.0 µl\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eConjugate:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"short-description\"\u003e\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\" class=\"short-description\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" class=\"short-description\"\u003eRecombinant, truncated Class I soluble Human Leukocyte Antigen (sHLA), naturally folded and glycosylated, loaded with endogenous peptides.\u003c\/p\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attributes\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALLELE NAME\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA*02:01, B2m\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eSEROLOGICAL NAME\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA2\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALLELE FREQUENCY - WORLDWIDE POPULATION\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e12.0%\/–\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALTERNATIVE NAMES\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA*0201, A0201, A*02, A02, A2\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eRECOMMENDED ANTIBODIES\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eW6\/32, Anti-B2m\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eAPPLICATIONS\u003c\/label\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eHLA Sandwich ELISAs, HLA Direct ELISAs, HLA-Sera Antibody Assays, HLA Controls in ELISA Assays, HLA Assay Standards, HLA Blocking Assays, HLA Neutralizing Assays, HLA Competition Assays, HLA Bead Assays, HLA Immunization Procedures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 234px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eConjugate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComplexity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eMonomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHLA Species\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHost Cell Line Species\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eClone Designation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eOB11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eTail Specification\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eVLDL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eProtein Format\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecombinant, truncated, soluble, non-modified, non-labeled, endogenously loaded\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eStorage Temperature\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e4˚C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1000.0 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003ePBS\/0.02% Sodium Azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003epH\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eResearch Use Only [RUO]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAuthentication Method\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSequence Based Typing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eSequences\u003c\/h2\u003e\n\u003ch3\u003eALPHA CHAIN SEQUENCE\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eGSHSMRYFFT SVSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASQRMEPRAP WIEQEGPEYW DGETRKVKAH SQTHRVDLGT LRGYYNQSEA GSHTVQRMYG CDVGSDWRFL RGYHQYAYDG KDYIALKEDL RSWTAADMAA QTTKHKWEAA HVAEQLRAYL EGTCVEWLRR YLENGKETLQ RTDAPKTHMT HHAVSDHEAT LRCWALSFYP AEITLTWQRD GEDQTQDTEL VETRPAGDGT FQKWAAVVVP SGQEQRYTCH VQHEGLPKPL TLRSVVSTDD DLA\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eBETA CHAIN SEQUENCE\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eIQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cbr\u003e\u003c\/h2\u003e","brand":"hlaprotein","offers":[{"title":"Default Title","offer_id":43193412878517,"sku":"A0201","price":773.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a0201_hla1_3d_r_2.png?v=1732874223"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    border-left: 1px solid rgba(255, 255, 255, 0.2);\n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    border-left: 4px solid #0056b3;\n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    border-left: 4px solid #0056b3;\n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e⚡ Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🕒 Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🎯 Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \u003cspan class=\"shentek-title-bar\"\u003e\u003c\/span\u003e\r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n                        \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n                        \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n                        \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n                        \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash steps\u003c\/td\u003e\r\n                        \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n                        \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n                        \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n                    \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n                    \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n                    \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-host-cell-rna-sample-preparation-kit-shentek™","title":"Residual Host Cell RNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item 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30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e⚡ Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🕒 Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🎯 Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature; 2-8 °C; -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \u003cspan class=\"shentek-title-bar\"\u003e\u003c\/span\u003e\r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell RNA Sample Preparation Kit is a magnetic particle-based RNA extraction kit for isolation of residual host cell RNA from biological products (e.g., E. coli, 293T). The kit is compatible with manual workflows and automated extraction on the rHCDpurify system. Extracted RNA is suitable for downstream RNA quantitation (real-time qPCR) and other nucleic acid-based assays. The kit includes reagents for protein digestion (Proteinase K), binding, washing, magnetic particles, RNase inhibitor, glycogen carrier, and elution reagents. The kit is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eExtraction of residual host cell RNA from biological samples for downstream RNA quantitation (qPCR)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eExtraction method\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMagnetic particle-based RNA binding, wash and elution\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n                        \u003ctd\u003eTypical: add 100 µL sample + 110 µL Proteinase K digestion solution; incubate 55 °C for 60 min; machine max digestion volume 300 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K dosing guidance\u003c\/td\u003e\r\n                        \u003ctd\u003eFor sample protein concentration 0–100 mg\/mL: 10 µL Proteinase K per sample; for 100–200 mg\/mL: 20 µL Proteinase K per sample (volumes mixed with Proteinase K Buffer as tabled)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding\u003c\/td\u003e\r\n                        \u003ctd\u003ePer sample: add 10 µL 5M NaCl + 220 µL working binding solution; add 200–400 µL isopropanol (≈1\/3–1\/2 total volume); add 10–15 µL magnetic particles; mix 3–5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWashing\u003c\/td\u003e\r\n                        \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL; air-dry magnetic particles 30 s–3 min to remove residual ethanol\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAdd 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v); incubate 50 °C for 5 min with intermittent vortexing; separate beads and recover eluate\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n                        \u003ctd\u003eUse 10–15 µL per sample; vortex to re-suspend before use; recommended storage 2–8 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWorking binding prep\u003c\/td\u003e\r\n                        \u003ctd\u003ePer sample working binding: 200 µL Binding solution + 10 µL diluted Precipitation solution I (1:99 in Dilution solution) + 10 µL Glycogen\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eIdeal sample pH 6.0–8.0; adjust with 1 M HCl or 1 M NaOH if pH \u0026lt;5.0 or \u0026gt;9.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures are stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility \/ equipment\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with rHCDpurify automated system and manual extraction; requires real-time PCR system for downstream quantitation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic particle-based separation:\u003c\/strong\u003e Efficient magnetic bead capture of RNA for high recovery and clean eluates suitable for qPCR\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAutomated and manual compatibility:\u003c\/strong\u003e Validated for use with rHCDpurify instrument and a manual workflow\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit for RNA extraction:\u003c\/strong\u003e Includes Proteinase K digestion reagents, binding\/wash\/elution buffers, magnetic particles, RNase inhibitor and glycogen carrier\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for residual host cell RNA:\u003c\/strong\u003e Optimized procedure for extraction of residual RNA from biological products and plasmid preparations\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStable shelf life:\u003c\/strong\u003e Components stored at proper temperatures are stable up to 24 months\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of residual host cell RNA from biopharmaceutical samples (e.g., E. coli, 293T)\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for RNA quantitation by real-time qPCR\u003c\/li\u003e\r\n                    \u003cli\u003ePreparation of plasmid DNA samples with residual host RNA assessment\u003c\/li\u003e\r\n                    \u003cli\u003eRoutine QC workflows in research and development laboratories\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND016) 20 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND014) 30 mL × 1 (requires addition of 40 mL ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution solution (NND061) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution solution (NND062) 12 mL × 2\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5M NaCl (NND040) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase inhibitor (NND060) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePrecipitation solution I (NND003) 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based RNA extraction (manual or rHCDpurify automated)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only. Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: gloves, protective eyewear, mask and lab clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 40 mL ethanol to Wash buffer A before first use; prepare 70% ethanol Wash buffer B from Dilution solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare working elution solution by mixing RNase inhibitor : Elution solution = 1 : 200 (v:v) in RNase-free area.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare diluted Precipitation solution I at 1:99 (Precipitation solution I : Dilution solution) before preparing working binding solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ideal sample pH is 6.0–8.0. Adjust samples to neutral pH if outside range.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not over-dry magnetic particles; over-drying may reduce elution efficiency.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform downstream assays the same day as extraction to ensure accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• When removing supernatant, do not disturb or aspirate magnetic bead pellet; use low-retention tips and centrifuge as instructed.\u003c\/p\u003e\r\n                    \u003cp\u003e• UV sterilize rHCDpurify instrument interior (suggested 15 minutes) before runs; allow \u0026gt;30 min between extractions if required.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201205.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201205.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What downstream assays are compatible with the extracted RNA?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted RNA is suitable for RNA quantitation by real-time qPCR and other nucleic-acid based assays. Use appropriate DNase treatment if required by your assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare Wash buffer A and B before first use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 40 mL of ethanol to Wash buffer A (NND014) before first use. Prepare Wash buffer B by mixing Dilution solution (NND062) with ethanol to make 70% ethanol.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended elution volumes and conditions?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v). Incubate at 50 °C for 5 minutes with intermittent vortexing, then separate beads and recover eluate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this kit be used on an automated platform?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify automated extraction system (program rHCR-1201205) as well as manual extraction workflows.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45189191270581,"sku":"1201205","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_RNA_Sample_Preparation_Kit.jpg?v=1742293165"},{"product_id":"hla-dpa1-rabbit-polyclonal-antibody","title":"HLA-DPA1 rabbit polyclonal antibody","description":"\n    \u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n    \u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n      \u003ctbody\u003e\n    \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eHLA-DPA1 rabbit polyclonal antibody\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eAlternative names\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e50 μl\/100 μl\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit polyclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eWB, IHC\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eRabbit\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eClone type\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit polyclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eTarget Background\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eSwissprot No\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eP20036\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eGene Accession\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNP_291032\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Predicted band size\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e29 kDa\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Recommended dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e500-2000\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC predicted cell location\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003ePredicted cell location: Cell membrane\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003ePositive control: Human tonsil\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC Recommed dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eRecommended dilution: 40-200\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n    \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003e-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol\u003c\/p\u003e\n      \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eWB PIC Description\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003eP111841-WB-1-P\u003c\/p\u003e\n      \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eIHC PIC Description\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp style=\"margin-bottom: 50px;\"\u003eImmunohistochemical analysis of paraffin-embedded Human tonsil tissue or HLA-DPA1 antigen-treated (Neutralization experiment) using P111841(HLA-DPA1 Antibody) at dilution 1\/35\u003c\/p\u003e\n      ","brand":"KleanAB","offers":[{"title":"50 μl","offer_id":44604378677429,"sku":"P111841-50 μl","price":154.0,"currency_code":"USD","in_stock":true},{"title":"100 μl","offer_id":44604378710197,"sku":"P111841-100 μl","price":257.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P111841-WB-1-P_d5d0e105-93b6-4a18-a75f-9e1ad0bd5e9e.jpg?v=1739286044"},{"product_id":"nfkbia-phospho-ser32-rabbit-monoclonal-antibody","title":"NFKBIA (phospho-Ser32) rabbit monoclonal antibody","description":"\n    \u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n    \u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n      \u003ctbody\u003e\n    \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNFKBIA (phospho-Ser32) rabbit monoclonal antibody\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eAlternative names\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e50 μl\/100 μl\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit monoclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eWB\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eRabbit\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eClone type\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit monoclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eTarget Background\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eThis gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B\/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eSwissprot No\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eP25963\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eGene Accession\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e4792\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Predicted band size\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e36 kDa\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Recommended dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e500-2000\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC predicted cell location\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC Recommed dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n    \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003e-20°C, 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n      \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eWB PIC Description\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003eP291029-WB-1-P\u003c\/p\u003e\n      ","brand":"KleanAB","offers":[{"title":"50 μl","offer_id":44604753477813,"sku":"P291029-50 μl","price":257.0,"currency_code":"USD","in_stock":true},{"title":"100 μl","offer_id":44604753510581,"sku":"P291029-100 μl","price":411.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P291029-WB-1-P_8170cbab-1a17-4f7e-a731-ce2ab99e49cd.jpg?v=1739294687"},{"product_id":"nfkbia-phospho-ser36-rabbit-monoclonal-antibody","title":"NFKBIA (phospho-Ser36) rabbit monoclonal antibody","description":"\n    \u003ch2 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eSpecification\u003c\/span\u003e\u003c\/b\u003e\u003c\/h2\u003e\n    \u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n      \u003ctbody\u003e\n    \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eFull name\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eNFKBIA (phospho-Ser36) rabbit monoclonal antibody\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eAlternative names\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e50 μl\/100 μl\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eReactivity\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit monoclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eWB\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eRabbit\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eClone type\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003erabbit monoclonal\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eTarget Background\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eThis gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B\/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eSwissprot No\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eP25963\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eGene Accession\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e4792\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Predicted band size\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e36 kDa\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eWB Recommended dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003e500-2000\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC predicted cell location\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC positive control\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003ctr\u003e\n        \u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003eIHC Recommed dilution\u003c\/strong\u003e\u003c\/td\u003e\n        \u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003enan\u003c\/strong\u003e\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n    \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003e-20°C, 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n      \n      \u003ch2 style=\"margin-top: 50px;\"\u003e\u003cspan\u003eWB PIC Description\u003c\/span\u003e\u003c\/h2\u003e\n      \u003cp\u003eP291030-WB-1-P\u003c\/p\u003e\n      ","brand":"KleanAB","offers":[{"title":"50 μl","offer_id":44604753641653,"sku":"P291030-50 μl","price":257.0,"currency_code":"USD","in_stock":true},{"title":"100 μl","offer_id":44604753707189,"sku":"P291030-100 μl","price":411.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/P291030-WB-1-P_f611f876-6e48-4420-8925-a3817b973dca.jpg?v=1739294691"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-vaccines-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit For Vaccines SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    border-left: 1px solid rgba(255, 255, 255, 0.2);\n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    border-left: 4px solid #0056b3;\n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    border-left: 4px solid #0056b3;\n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e⚡ Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🕒 Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🎯 Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C \u0026amp; -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \u003cspan class=\"shentek-title-bar\"\u003e\u003c\/span\u003e\r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit For Vaccines is a magnetic particle–based nucleic acid extraction kit specifically optimized for vaccine products. It is designed to stably and efficiently recover trace amounts of residual host-cell DNA from finished vaccines (e.g., Vero cell–derived rabies vaccines). The kit provides an integrated workflow when used with SHENTEK® host cell DNA qPCR detection kits and supports manual extraction or automated extraction using the rHCDpurify® system. Special sample processing methods can be provided for samples containing aluminum adjuvants or dextran.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number\u003c\/td\u003e\r\n                        \u003ctd\u003eSK030206DM50\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n                        \u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL test sample (per extraction)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDigestion reagents per sample\u003c\/td\u003e\r\n                        \u003ctd\u003e10 µL 5M NaCl, 30 µL Sample buffer (NND029), 15 µL Proteinase K (NND023)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDigestion conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eIncubate at 55 °C for 60 minutes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\r\n                        \u003ctd\u003e200 µL Binding solution (NND017) + 200 µL isopropanol + 10 µL Magnetic particles (NND030)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding procedure\u003c\/td\u003e\r\n                        \u003ctd\u003eVortex 5 minutes (medium speed); magnetic separation 3–5 minutes\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n                        \u003ctd\u003e700 µL Wash buffer A (with ethanol added) then 700 µL Wash buffer B (70% ethanol)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution volume and conditions\u003c\/td\u003e\r\n                        \u003ctd\u003e50–100 µL Elution buffer (NND019); incubate at 70 °C for 7 minutes (vortex 2–3 times during incubation)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\r\n                        \u003ctd\u003eResuspend magnetic particles before use; allow particles to separate 3–5 minutes on magnet; air-dry 1–3 minutes before elution\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible automation\u003c\/td\u003e\r\n                        \u003ctd\u003erHCDpurify® automated extraction system (program rHCD-06DM50 \/ HCD-06DM50)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd\u003eKit components stored under indicated conditions are stable up to 24 months (check label expiration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCritical sample pH range\u003c\/td\u003e\r\n                        \u003ctd\u003epH 6.0–8.0 required; adjust with 1M HCl or 1M NaOH if outside range\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecommended controls\u003c\/td\u003e\r\n                        \u003ctd\u003eNegative Control Sample (NCS) and Extraction Recovery Control (ERC) (ERC spike recommended 2–10× amount found in unspiked sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use \/ Compliance\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — sample preparation for downstream host cell DNA qPCR assays\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation technology:\u003c\/strong\u003e Efficient capture and concentration of trace DNA using magnetic particles for high recovery.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eVaccine-optimized workflow:\u003c\/strong\u003e Formulation and protocol optimized for finished vaccine matrices (e.g., Vero cell–derived rabies vaccine). Specific processing available for aluminum adjuvants or dextran-containing samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eIntegrated with qPCR detection:\u003c\/strong\u003e Designed to work with SHENTEK® host cell DNA qPCR detection kits to provide a complete sample-to-result workflow.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and automated compatibility:\u003c\/strong\u003e Protocols for manual extraction and for automated processing on the rHCDpurify® system (96 deep-well format) to support throughput needs.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eLow-volume, trace DNA recovery:\u003c\/strong\u003e Optimized to obtain trace amounts of residual host-cell DNA stably and reproducibly from complex vaccine matrices.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eSample pretreatment for residual host cell DNA testing in finished vaccines (e.g., Vero cell–derived rabies vaccine)\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing during vaccine manufacture\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation prior to SHENTEK® host cell DNA qPCR assays\u003c\/li\u003e\r\n                    \u003cli\u003eAutomated high-throughput residual DNA extraction using rHCDpurify® system\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eSample buffer (NND029)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based nucleic acid extraction for downstream qPCR detection (manual or rHCDpurify automated)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Follow Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use separate laboratory areas for negative reagent preparation, positive\/sample processing, and PCR amplification to prevent cross-contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure ambient temperature is not lower than 22 °C before starting experiments.\u003c\/p\u003e\r\n                    \u003cp\u003e• Centrifuge tubes before opening to avoid contamination; handle carefully to avoid splashing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Change gloves regularly and use dedicated lab coats\/masks\/hair covers in different areas.\u003c\/p\u003e\r\n                    \u003cp\u003e• Dispose of used tips and liquid waste per regulations; do not open PCR amplification products in non-designated areas.\u003c\/p\u003e\r\n                    \u003cp\u003e• During magnetic separation, rotate tubes slowly to accelerate particle aggregation; avoid disturbing particles when removing supernatant.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid over-drying magnetic particles after wash (over-drying can cause incomplete elution).\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure no magnetic particles or liquid remain on tube caps or walls; if present, briefly centrifuge.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform DNA detection on the same day as extraction to ensure accuracy.\u003c\/p\u003e\r\n                    \u003cp\u003e• Before automated runs, confirm 96-well plate and sleeve are secured; UV-sterilize the instrument interior and wipe with 75% ethanol before and after runs.\u003c\/p\u003e\r\n                    \u003cp\u003e• Minimum interval between two extractions on rHCDpurify® should be 30 minutes; UV sterilize instrument for at least 15 minutes between runs.\u003c\/p\u003e\r\n                    \u003cp\u003e• Collect more than 40 µL of eluate for downstream assay; transfer eluate immediately after automated run to new microcentrifuge tubes.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030206DM50.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030206DM50.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is required per extraction?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eUse 100 µL test sample per extraction. The protocol specifies addition of 10 µL 5M NaCl and 30 µL Sample buffer prior to Proteinase K digestion.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the magnetic particles and Proteinase K be stored?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Store Proteinase K at -20 °C. Other buffers are stored at room temperature; kit components are stable under indicated conditions for up to 24 months (check label expiration).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit be used with automated extraction instruments?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify® automated system (program: rHCD-06DM50 \/ HCD-06DM50) using a 96 deep-well plate layout; the manual also provides program settings and plate layout guidance.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe low recovery of nucleic acids?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include missing ethanol in Wash buffer A, magnetic particles sticking to tube walls, low ionic strength, out-of-range sample pH, or incorrect storage of magnetic particles. Solutions: add ethanol to Wash buffer A per instructions, vortex\/heat to release particles from tube walls (70 °C for 2 min) and re-vortex, adjust ionic strength with 5M NaCl, adjust sample pH to neutral, and store magnetic particles at 2–8 °C (do not store at -20 °C).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171074105525,"sku":"SK030206DM50","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_Vaccines.jpg?v=1742277270"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-manual-operation-shentek®","title":"Residual Host Cell DNA Sample Preparation Kit (For manual operation) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    border-left: 1px solid rgba(255, 255, 255, 0.2);\n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    border-left: 4px solid #0056b3;\n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    border-left: 4px solid #0056b3;\n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e⚡ Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🕒 Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e🎯 Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \u003cspan class=\"shentek-title-bar\"\u003e\u003c\/span\u003e\r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic-particle based sample preparation kit designed for efficient and reproducible recovery of trace residual host-cell DNA from a wide range of biological products and complex sample matrices across multiple manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended to be used with SHENTEK® host cell DNA quantitation and size analysis kits. The kit supports multiple host cell types (CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA, etc.), and includes reagents for Proteinase K digestion, DNA binding, washing and elution, with magnetic particles for nucleic acid capture.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input (typical)\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL sample per extraction (procedure described: add 100 µL sample + 10 µL 5M NaCl before digestion)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003ePrepare Proteinase K digestion solution in Proteinase K Buffer. For samples with 0–100 mg\/mL protein add 10 µL Proteinase K per 100 µL buffer; for 100–200 mg\/mL protein add 20 µL Proteinase K per 100 µL buffer. Incubate digestion at 55 °C for 60 min.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n                        \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA. Note: Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particles usage\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAdd 10 µL magnetic particles per sample. Vortex particles 5 seconds to fully resuspend before use. Store magnetic particles at 2–8 °C.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAlcohol addition for binding\u003c\/td\u003e\r\n                        \u003ctd\u003eAfter binding buffer, add 200 µL isopropanol per sample to facilitate binding.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding incubation\u003c\/td\u003e\r\n                        \u003ctd\u003eVortex tubes vertically at medium speed for 5 minutes to bind nucleic acids to magnetic particles.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash steps\u003c\/td\u003e\r\n                        \u003ctd\u003eWash once with 700 µL Wash buffer A (ethanol to be added as instructed), then wash with 700 µL Wash buffer B (70% ethanol prepared separately). Spin 10 seconds between steps and use magnetic stand to separate beads.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBead drying\u003c\/td\u003e\r\n                        \u003ctd\u003eAir-dry magnetic pellet at room temperature for 30 seconds to 3 minutes (environment dependent) to remove residual ethanol; avoid over-drying which hinders resuspension.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 50–100 µL pre-warmed (70 °C) Elution buffer, vortex 5 seconds, incubate at 70 °C for 7 minutes with intermittent vortexing. Centrifuge 1 min, separate beads on magnetic stand and transfer eluate.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTypical centrifugation\u003c\/td\u003e\r\n                        \u003ctd\u003eQuick spins of 10 seconds are used throughout to collect liquids from caps\/walls; final centrifuge after elution 1 minute.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReagent storage conditions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eWash Buffer A: room temperature (add 40 mL anhydrous ethanol before use); Binding solution \/ Elution buffer \/ Dilution buffer \/ Proteinase K Buffer: room temperature (as labeled); Magnetic particles: 2–8 °C; Proteinase K: (no explicit temperature on label in table) - recommended follow label; Glycogen: -20 °C; Yeast tRNA: per label.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eShelf life\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under their appropriate conditions for up to 24 months (check label expiration dates).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eAdjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction if sample pH \u0026lt;5 or \u0026gt;9.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n                        \u003ctd\u003eUse Negative Control Sample (NCS) processed in parallel and Sample Extraction Recovery Control (ERC) spiked at 2–10× the amount quantified in the unspiked sample to monitor extraction recovery and accuracy.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic-particle based extraction:\u003c\/strong\u003e Magnetic particle separation technology enables efficient capture and reproducible recovery of trace residual DNA from complex biological matrices.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad host-cell compatibility:\u003c\/strong\u003e Validated for multiple host cells including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Compatible with manual preparation or automated extraction using the rHCDpurify instrument.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eOptimized for downstream quantitation:\u003c\/strong\u003e Extracted DNA is suitable for SHENTEK® host cell DNA quantitation and size analysis kits and analysis on real-time PCR systems.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-configured reagents for 100 extractions:\u003c\/strong\u003e Complete reagent set supplied for 100 DNA extractions, with specific buffers and additives (Glycogen, tRNA) for improved recovery of low-abundance DNA.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eResidual host cell DNA extraction and purification from biologics and process intermediates\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for host-cell DNA quantitation by qPCR\u003c\/li\u003e\r\n                    \u003cli\u003eProcess monitoring for biologics and vaccine manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eSize analysis and downstream molecular assays of residual DNA\u003c\/li\u003e\r\n                    \u003cli\u003eExtraction from diverse matrices including upstream and downstream process samples\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash Buffer A (NND014) 30 mL × 1 bottle (add 40 mL anhydrous ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding Solution (NND016) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution Buffer (NND018) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution Buffer (NND021) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND031) 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label); prepare fresh digestion solution as instructed\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eFollow label (store frozen or per label)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle separation (sample extraction)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, clothing, and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash Buffer A before first use; prepare 70% Wash Buffer B separately and label.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare and use 100% isopropanol for binding step as instructed.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure reagents are clear before use; if cloudy, heat at 37 °C until clear and mix well.\u003c\/p\u003e\r\n                    \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) if outside range to avoid affecting extraction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n                    \u003cp\u003e• Avoid over-drying magnetic pellet; air-dry 30 seconds to 3 minutes depending on environment.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform downstream assays on the same day after nucleic acid extraction for accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store magnetic particles at 2–8 °C; storage below -18 °C may degrade performance.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use low-retention filter tips and regularly calibrate pipettes to ensure accurate spiking and aspiration.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030203D100.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is recommended per extraction?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes using 100 µL of sample per extraction. Adjustments may be made with proportional reagent preparation; follow kit guidelines for dilution and recovery controls.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I use this kit for E. coli and yeast DNA extraction?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit supports E. coli and yeast DNA extraction; note that Yeast tRNA should not be added to the Binding buffer when extracting E. coli or yeast DNA.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended incubation conditions for Proteinase K digestion?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution according to sample protein concentration and incubate at 55 °C for 60 minutes as described in the protocol.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store the magnetic particles and other reagents?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Glycogen is stored at -20 °C. Other liquid buffers are stored at room temperature as labeled. The kit components can be stored under appropriate conditions for up to 24 months; always check expiration dates on labels.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186936340661,"sku":"SK030203D100","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ResidualHostCellDNASamplePreparationKit_Formanualoperation_-SHENTEK.jpg?v=1742276761"},{"product_id":"actcel-t-cell-activation-reagent-1","title":"ActCel T Cell Activation Reagent","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo – Mobile Table Adaptation\n  ========================== *\/\n\n  .biofargo-table-wrapper{\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n    margin: 20px 0;\n  }\n\n  .biofargo-spec-table{\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #dee2e6;\n    background: #fff;\n    font-size: 14px;\n    min-width: 600px;\n  }\n\n  .biofargo-spec-table td{\n    border: 1px solid #ddd;\n    padding: 10px;\n    text-align: center;\n    vertical-align: middle;\n    white-space: normal;\n    word-break: break-word;\n  }\n\n  @media (max-width: 600px){\n    .biofargo-table-wrapper{\n      border: 1px solid #eee;\n      border-radius: 8px;\n      padding: 6px;\n      background: #fff;\n    }\n\n    .biofargo-spec-table{\n      width: max-content;\n      min-width: 720px;\n      display: inline-table;\n    }\n\n    .biofargo-spec-table td{\n      white-space: nowrap;\n    }\n  }\n\n  .biofargo-app-grid{\n    display: grid;\n    grid-template-columns: 1fr 1fr;\n    gap: 20px;\n  }\n\n  @media (max-width: 640px){\n    .biofargo-app-grid{\n      grid-template-columns: 1fr;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eActCel T Cell Activation Reagent is designed for the activation and expansion of T cells in vitro, particularly in the production of T cell therapies such as CAR-T and TCR-T.\u003c\/p\u003e\n\u003cp\u003eThis reagent enables the rapid and efficient activation and expansion of T cells, supporting the production of the large-scale cell quantities required for clinical applications.\u003c\/p\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; background-color: #f7f9fb; font-weight: bold;\"\u003eReactive Species\u003c\/td\u003e\n\u003ctd style=\"width: 50%;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color: #f7f9fb; font-weight: bold;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 2 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background-color: #f7f9fb; font-weight: bold;\"\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eBrown liquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e50 nm nano-scale particles\u003c\/strong\u003e\u003cbr\u003e50 nm particle size enables gentle and efficient T cell activation, minimizing physical stress while maintaining high activation efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReady-to-use formulation\u003c\/strong\u003e\u003cbr\u003eSupplied as a ready-to-use reagent with no pre-cleaning or pre-coating steps required, simplifying workflow and reducing hands-on time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStable and uniform suspension\u003c\/strong\u003e\u003cbr\u003eUniform particle distribution ensures consistent activation performance and reproducible T cell expansion across batches.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNo dissociation step required\u003c\/strong\u003e\u003cbr\u003eEliminates the need for bead removal or dissociation after activation, streamlining downstream culture and processing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDual-grade availability (RUO \u0026amp; GMP)\u003c\/strong\u003e\u003cbr\u003eAvailable in both RUO and GMP-grade formats to support early research, process development, and regulated manufacturing workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCost-efficient solution\u003c\/strong\u003e\u003cbr\u003eDesigned as a cost-effective alternative for scalable T cell activation and expansion applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv style=\"margin-top: 12px;\" class=\"biofargo-app-grid\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell activation and expansion\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eRapid and efficient activation of human T cells in vitro, supporting robust proliferation and functional expansion.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCAR-T and TCR-T manufacturing\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSuitable for activation steps in CAR-T and TCR-T workflows, enabling scalable production of therapeutic T cells.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell therapy process development\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSupports optimization and standardization of T cell activation protocols during process development and scale-up.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eImmunotherapy research\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eApplicable to T cell functional studies and development of cell-based immunotherapy platforms.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-ActCel_T_Cell_Activation_Reagent.pdf?v=1772523218\" target=\"_blank\"\u003e📄 GMP-TL6001 Data Sheet\u003c\/a\u003e \n  \u003ca style=\"display: inline-block; padding: 10px 20px; background-color: #0056b3; color: white; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-ActCel_T_Cell_Activation_Reagent.pdf?v=1772523218\" target=\"_blank\"\u003e📄 GMP-TL6001 SDS\u003c\/a\u003e\n   \u003ca style=\"display: inline-block; padding: 10px 20px; background-color: #0056b3; color: white; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-BD2508001-GMP-TL6001-1000.pdf?v=1772523219\" target=\"_blank\"\u003e📄 GMP-TL6001 COA\u003c\/a\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eAttention\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5; border-left: 5px solid #ff4d4f; padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eFor research use and GMP manufacturing workflows only. Not for direct clinical administration.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"GMP \/ 1mL","offer_id":51375837839541,"sku":"GMP-TL6001-1000","price":285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG-GMP-TL6001-1000_1.png?v=1770362280"},{"product_id":"natural-killer-cells-induction-culture-kit-3-0","title":"Natural Killer Cells Induction Culture Kit 3.0","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eNatural Killer Cells Induction Culture Kit 3.0 is a GMP-grade in vitro induced expansion culture kit, which is suitable for natural killer cell expansion without feeder cells. The kit includes NK Cell Coating Solution, NK Cell Induction Solution, NK Cell Activator, NK Cell Culture Supplement and N300 Serum-Free Medium for NK Cells. This product has stable inter-batch quality and is used to induce and expand NK cells from umbilical cord blood in vitro.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5658%; text-align: center; vertical-align: middle;\"\u003eProduct\u003cbr\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 10.9885%; text-align: center; vertical-align: middle;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 9.09571%; text-align: center; vertical-align: middle;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eComponent Name\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003eComponent\u003cbr\u003eSize\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5658%; text-align: center; vertical-align: middle;\" rowspan=\"15\"\u003eNatural\u003cbr\u003eKiller\u003cbr\u003eCells\u003cbr\u003eInduction\u003cbr\u003eCulture\u003cbr\u003eKit 3.0\u003c\/td\u003e\n\u003ctd style=\"width: 10.9885%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e6813551\u003c\/td\u003e\n\u003ctd style=\"width: 9.09571%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e3 L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Coating Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e300 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Induction Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e600 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Activator\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e3 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Culture Supplement\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e42 mL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eN300 Serum-Free Medium for NK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 L\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e3 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.9885%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e6813552\u003c\/td\u003e\n\u003ctd style=\"width: 9.09571%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e2 L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Coating Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Induction Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Activator\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e2 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Culture Supplement\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e28 mL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eN300 Serum-Free Medium for NK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 L\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e2 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 10.9885%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e6813553\u003c\/td\u003e\n\u003ctd style=\"width: 9.09571%; text-align: center; vertical-align: middle;\" rowspan=\"5\"\u003e1 L\/kit\u003c\/td\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Coating Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Induction Solution\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Activator\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 pcs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eNK Cell Culture Supplement\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e14 mL\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 34.0169%; text-align: center; vertical-align: middle;\"\u003eN300 Serum-Free Medium for NK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 L\u003c\/td\u003e\n\u003ctd style=\"width: 16.6667%; text-align: center; vertical-align: middle;\"\u003e1 bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Conditions and Validity Period\u003c\/h2\u003e\n\u003cp\u003eNK Cell Coating Solution, NK Cell Induction Solution, NK Cell Activator, NK Cell Culture\u003cbr\u003eSupplement: Store at -15℃ to -25℃, valid for one year.\u003c\/p\u003e\n\u003cp\u003eN300 Serum-Free Medium for NK Cells: Keep away from light at 2 to 8℃, valid for one year.\u003c\/p\u003e\n\u003ch2\u003eDirections for Use\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eTake 1 L system (Cat.No.6813553) as an example:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3\u003eSample Requirements\u003c\/h3\u003e\n\u003cp\u003eFresh or cryopreserved human umbilical cord blood mononuclear cells (UBMCs) .\u003c\/p\u003e\n\u003ch3\u003ePreparation\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eFlask Coating\u003c\/p\u003e\n\u003cp\u003eOn Day -1, mix 100 μL NK Cell Coating Solution with 3 mL PBS thoroughly and add the mixture into a T25 flask. Shake the T25 flask horizontally to cover the mixture over the bottom of the flask and coat it overnight at 4℃ (coat for 2 h at 37℃ in case of an emergency condition). After the coating, remove excess coating solution and wash the bottom of the flask gently with 10 mL PBS. Be careful not to scour the bottom of the culture flask directly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare Amplification Medium for NK Cells\u003c\/p\u003e\n\u003cp\u003eOn Day 0, add 1 vial of 500 μL NK Cell Activator to 1 L of N300 Serum-Free Medium for NK Cells to prepare the Amplification Medium for NK Cells. Keep the medium away from light at 2 to 8℃, valid for 3 weeks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare Induction Medium for NK Cells\u003c\/p\u003e\n\u003cp\u003eOn Day 0, add 200 μL NK Cell Induction Solution to 25 mL of Amplification Medium for NK Cells to prepare the Induction Medium for NK Cells which is used on D0 and D3 of NK cell culture. Keep the medium away from light at 2 to 8℃.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eNK Cell Culture\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 0, inoculate UBMCs with 10 mL Induction Medium for NK Cells (containing 10% heat-inactivated cord blood plasma and 10% NK Cell Culture Supplement) at a density of 2.5–3.0×10^6 cells\/mL into the T25 coated flask. After shaking, incubate the cells in a 37℃ incubator containing 5% CO2.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 3, observe the cell status, then supplement 15 mL of fresh Induction Medium for NK Cells (containing 10% heat-inactivated cord blood plasma and 10% NK Cell Culture Supplement) to the original T25 flask gently. Do not disturb the cells at the bottom.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNote: T25 flask should be placed slightly tilted to avoid liquid overflow.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 5, observe the cell status and count the number. If the cell density is less than 1.4×10^6 cells\/mL, add 25 mL of Amplification Medium for NK Cells (containing 5% heat-inactivated cord blood plasma and 5% NK Cell Culture Supplement) to the culture. If the cell density is greater than 1.4×10^6 cells\/mL, add 55 mL of Amplification Medium for NK Cells (containing 5% heat-inactivated cord blood plasma and 5% NK Cell Culture Supplement).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 7, observe the cell status and count the number. Add Amplification Medium for NK Cells (containing 2.5% heat-inactivated cord blood plasma and 2.5% NK Cell Culture Supplement) to the culture according to medium supplementation reference procedure in Table 1.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 9, observe the cell status and count the number. Add Amplification Medium for NK Cells (containing 1% NK Cell Culture Supplement) to the culture according to medium supplementation reference procedure in Table 1.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 11, observe the cell status and count the number. Add Amplification Medium for NK Cells (containing 0.5% NK Cell Culture Supplement) to the culture according to medium supplementation reference procedure in Table 1.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 14, observe the cell status and count the number. Add the rest of Amplification Medium for NK Cells (containing 0.5% NK Cell Culture Supplement) to the culture according to medium supplementation reference procedure to achieve a final culture volume of 1000 mL.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOn Day 16–18, observe the cell status, take photos, count cell number and harvest cells for subsequent use.\u003c\/p\u003e\n\u003cp\u003eNote:\u003c\/p\u003e\n\u003cp\u003e(1) Detect NK proportion on D0, D7 and D17 by flow cytometry, and the harvest time could be advanced or delayed appropriately according to the growth status of cells.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e(2) The result of in vitro expansion of NK cells derived from umbilical cord blood varies from donor to donor, and the above culture protocol is a relatively stable culture procedure after optimization. Due to differences in NK donors, NK cells may expand faster or slower than expected. It is recommended to carefully observe cell growth during the culture cycle and flexibly adjust the medium supplementation and harvest steps to ensure that the in vitro expansion performance of the kit can be achieved.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eProtocol for the Separation of UBMCs\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePour the anticoagulant blood into 50 mL centrifuge tubes, centrifuge at 700 g for 15 min (the acceleration is set to eighth gear and the deceleration is set to fourth gear). Then transfer the \u003cspan\u003eplasma layer to a new centrifuge tube. Inactivate the plasma at 56℃ for 30 min, then centrifuge \u003c\/span\u003e\u003cspan\u003eat 900 g for 10 min. Place supernatant at -20℃ for 20 min to cool down and centrifuge again at \u003c\/span\u003e\u003cspan\u003e900 g for 10 min. Afterwards, keep supernatant (i.e., heat-inactivated cord blood plasma) at 4℃ \u003c\/span\u003e\u003cspan\u003efor future use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAdd PBS in the same volume as inactivated plasma to the previous tube after the plasma was removed. Mix the tube thoroughly and add the Erythrocyte Sedimentation Solution in the ratio of 1:3 to 1:4 according to the volume of mixture in the tube. Mix the tube thoroughly and allow the tube to stand for about 30 min, until the interface between supernatant and RBC accounted for about 50% of the total volume, and collect the sedimentation supernatant into a 50 mL centrifuge tube and mix inversely.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAdd a certain volume of the Human Lymphocyte Separation Medium (the volume ratio of the separation medium to the sedimentation supernatant is 1:1) into the centrifuge tube, carefully layer the sedimentation supernatant over the separation medium, centrifuge at 800 g for 20 minutes at 20℃ (the acceleration and the deceleration are set to third gear) to obtain a high-purity mononuclear cell layer.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eCollect the UBMCs layer, wash with RPMI 1640 twice (250 g, 10 min, 20℃), and re-suspend UBMCs with medium. Count after dilution, then obtain the UBMCs.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1: Medium Supplementation Reference Procedure\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eDays\u003cbr\u003e\/d\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCulture\u003cbr\u003eFlask or\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd colspan=\"4\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eSupplementation\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eTotal\u003cbr\u003eVolume\u003cbr\u003e\/mL\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eNotes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eMedium\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eMedium\u003cbr\u003eVolume\u003cbr\u003e\/mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003ePlasma\u003cbr\u003eVolume\u003cbr\u003e\/mL\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eNK Cell\u003cbr\u003eCulture\u003cbr\u003eSupplement\u003cbr\u003e\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e-1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eT25\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCoat the T25 flask\u003cbr\u003eovernight at 4℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eT25\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eInduction\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1\u003cbr\u003e(10%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1\u003cbr\u003e(10%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eInoculation density is\u003cbr\u003e2.5–3.0 × 10\u003csup\u003e6\u003c\/sup\u003e\u003cbr\u003ecells\/mL.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eT25\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eInduction\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.5\u003cbr\u003e(10%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.5\u003cbr\u003e(10%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e25\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eDo not pipette cells\u003cbr\u003eto avoid interfering\u003cbr\u003ewith regular cell\u003cbr\u003eactivation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eT175\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAmplification\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e22.5\/49.5\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.25\/2.75\u003cbr\u003e(5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.25\/2.75\u003cbr\u003e(5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e50\/80\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eMaintain the cell\u003cbr\u003edensity at 0.5–1.0 × 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL after\u003cbr\u003esupplementation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eT175\/Cell\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAmplification\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e95\/152\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e2.5\/4\u003cbr\u003e(2.5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e2.5\/4\u003cbr\u003e(2.5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e150\/240\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eMaintain the cell\u003cbr\u003edensity at 0.5–1.0 × 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL after\u003cbr\u003esupplementation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCell\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAmplification\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e150\/160\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.5\/1.6\u003cbr\u003e(1%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e300\/400\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eMaintain the cell\u003cbr\u003edensity at about 1.0 × 10\u003csup\u003e6\u003c\/sup\u003e cells\/mL after\u003cbr\u003esupplementation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCell\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAmplification\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e250\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1.25\u003cbr\u003e(0.5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e550\/650\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e14\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCell\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAmplification\u003cbr\u003eMedium for\u003cbr\u003eNK Cells\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e450\/350\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e2.25\/1.75\u003cbr\u003e(0.5%)\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1000\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eAdd the rest of\u003cbr\u003emedium.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e16-18\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eCell\u003cbr\u003eCulture\u003cbr\u003eBag\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e\/\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003e1000\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center; vertical-align: middle;\"\u003eHarvest cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eIn Table 1, the percentage in parentheses refers to the percentage of plasma or NK Cell Culture\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAvoid repeated freezing and thawing of this product, and pay attention to operate aseptically during use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNK Cell Culture Supplement can support the efficient expansion of NK cells by adding to the medium in a certain proportion. Defrost it at 37℃ and prepare aliquots. Do not freeze and thaw the supplement repeatedly.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe recommended initial inoculation cell density is 2.5–3.0 × 10^6 cells\/mL. But the initial inoculation cell density can be increased to 3.0–3.5 × 10^6 cells\/mL when dealing with cryopreserved UBMCs . Too high or too low inoculation density would affect the effect of NK cell expansion.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDilute the NK Cell Coating Solution with PBS and coat the T25 flask overnight at 4℃ (coat for 2 h at 37℃ in case of an emergency condition).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBefore use, the medium should be equilibrated at room temperature. Otherwise, please transfer the estimated amount for use to a separate container and prewarm to 37℃. Do not place the whole bottle of medium at 37℃ for repeated prewarming.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell passage shall be carried out gently to avoid mechanical damages to cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e3 L system (Cat.No.6813551) is applicable to expansion of 7.5–9.0 × 10^7 UBMCs; 2 L system (Cat.No.6813552) is applicable to expansion of 5.0–6.0 × 10^7 UBMCs; 1 L system (Cat.No.6813553) is applicable to expansion of 2.5–3.0 × 10^7 UBMCs.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNK cells tend to form clumps during the early stage of culture and expansion. Gently add supplemental medium as much as possible and do not shake the cells to avoid interfering with the regular activation of NK cells. At the same time, note that Induction Medium for NK Cells is used for D0 and D3, followed by Amplification Medium for NK Cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWhen NK cells are cultured in the culture bag, it is recommended to fold the bag in half according to the culture volume.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6813551_6813552_6813553-Natural-Killer-Cells-Induction-Culture-Kit-3.0.pdf?v=1754032620\" type=\"application\/pdf\" target=\"_blank\"\u003e6813551、6813552、6813553-Natural-Killer-Cells-Induction-Culture-Kit-3.0.PDF \u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/33be2d22823dd171a4044489558fb2a7.doc?v=1754032704\"\u003e\u003cstrong\u003eNatural Killer Cells Induction Culture Kit 3.0.doc\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Natural_Killer_Cells_Induction_Culture_Kit_3.0_6813551_6813552_6813553-MSDS.pdf?v=1754044529\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eNatural Killer Cells Induction Culture Kit 3.0 6813551 6813552 6813553-MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/K-C021E-C0_Natural_Killer_Cells_Induction_Culture_Kit_NK3.0_1.pdf?v=1754044623\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eNatural Killer Cells Induction Culture Kit (NK3.0)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"1 L\/kit","offer_id":51456692322485,"sku":"6813553","price":1866.0,"currency_code":"USD","in_stock":true},{"title":"2 L\/kit","offer_id":51456692289717,"sku":"6813552","price":1280.0,"currency_code":"USD","in_stock":true},{"title":"3 L\/kit","offer_id":51456692256949,"sku":"6813551","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Natural_Killer_Cells_Induction_Culture_Kit_3.0_6853551.jpg?v=1754030718"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00002_249c8c04-711d-41ca-bd5f-bf8c2ba0dfa4.jpg?v=1776237262","url":"https:\/\/biofargo.com\/collections\/innate-adaptive-immunity.oembed","provider":"Biofargo","version":"1.0","type":"link"}